obj. 4 marker-assisted breeding pipeline implementation year 2 deliverables, challenges, year 3...
TRANSCRIPT
![Page 1: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/1.jpg)
OBJ. 4MARKER-ASSISTED BREEDING
PIPELINE IMPLEMENTATION
Year 2 Deliverables, Challenges, Year 3 goals
![Page 2: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/2.jpg)
GoalWhat
Impact
• Integrate modern genomics tools with traditional breeding approaches, systematically (8-stage MAB Pipeline)
• Enrich breeding material for favorable geneticsto efficiently increase likelihood and accuracyof identifying top performers
RosBREED
![Page 3: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/3.jpg)
What
To whom
Year 2 Deliverables
Impact
• Two Jewels (fast-tracked genetic tests) in routine use
• WSU apple and sweet cherry breeding programs
RosBREED• est. $80K saved from 2011 cherry seedling culling alone!
![Page 4: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/4.jpg)
What
Year 2 Deliverables
• Community Breeders Page - regular article in Newsletter
RosBREED
To whom
• Community Breeders
Impact
• Not evaluated – but represents early start on Yr 4 tech transfer
MAB Pipeline Nov‘10
apple Jewel use Feb‘11
Pipeline in action May‘11
peach Jewel use Aug‘11
cherry Jewel use Nov’11
![Page 5: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/5.jpg)
• Moving breeding programs forward together, keeping all on same page
- Requires success stories but also consistent& constant advice
Challenges and Plan for tackling
RosBREED
• Some enacted solutions:
- Community Breeders Page
- coordinated master datafiles
- much involved in other Team activities
...need more people on Team
![Page 6: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/6.jpg)
RosBREED
What
To whom
Planned Year 3 Deliverables
Intended Impact
• Routine “MAB Pipelining” by Demonstration Breeders
- First: fast-tracked markers into routine use- Soon after: New QTLs from PBA with genome scans
• Demonstration Breeders (Yr 3) other breeders (Yr 4)
• Success stories & experiences useful for Yr 4 “tech transfer” to all U.S. Rosaceae breeders.
![Page 7: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/7.jpg)
OBJ. 5EXTENSION
Year 2 Deliverables, Challenges, Year 3 goals
![Page 8: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/8.jpg)
RosBREED
Goals
• Breeders – adoption of Marker-Assisted Breeding
• Allied Scientists - utilization of newly generated science based knowledge, tools and databases
• Industry – better understanding of the power of DNA-informed breeding leads to new ways to jointly accelerate commercialization and impact of new cultivars
![Page 9: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/9.jpg)
What
To whom
Year 2 Deliverables
Impact
• Project-wide content – developed, disseminated and undertaken
• Project participants & stakeholders
• Stakeholders gain knowledge of project activities and deliverables.
78/130 recipients opened the Nov
2011 newsletter and Community
breeders said:
Stats: in 2011 the RosBREED website
was visited by 2,242 unique visitors,
from 79 countries. Since creation,
5,541 visits from US - ALL 50 states
“circulate to a wider
audience”
“it builds a sense of
community”
“RosBREED newsletter stands
out among other similar project
newsletters”
![Page 10: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/10.jpg)
What
To whom
Year 2 Deliverables
Impact
• Expansion of RosBREED network through presentations at meetings and informal gatherings – conducted
• Breeders, allied scientists, industry stakeholders
• Awareness and interest created at national and international meetings including “unveiling” of Jewels
ASHS 2011 poster and oral presentations
![Page 11: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/11.jpg)
What
To whom
Obj 5: Year 2 Deliverables
Impact
• Assist in surveys - Socio‐Economics Team grower mail‐in surveys and clicker surveys at grower meetings
• Market intermediaries and growers
• Growers help identify valuable Jewels and are more knowledgeable of their local breeding programs
![Page 12: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/12.jpg)
• Video content obtained, free computer software programs for editing existing videos is limited Experiment with softwares that have been used
successfully in other extension venues
• Obtain participation of a high percentage of Rosaceae
Community Breeders at the “MAB in Action” Workshop Hold Workshop the day prior to the ASHS meeting in
Miami; generate interest in the Workshop through publicizing success stories
Challenges and Plan for Tackling
Computer Demo:RosBREED Videos
![Page 13: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/13.jpg)
What
To whom
Planned Year 3 Deliverables
Intended Impact
• RosBREED Participatory Workshop: “Marker-Assisted Breeding in Action”, 30 July 2012 – promote, prepare & conduct
• Demonstration and Community breeders
• Comprehension by breeders of available knowledge, technologies and software for conducting routine MAB; application of MAB in their breeding programs
• Visit breeding programs on request to share RosBREED’s deliverables
![Page 14: OBJ. 4 MARKER-ASSISTED BREEDING PIPELINE IMPLEMENTATION Year 2 Deliverables, Challenges, Year 3 goals](https://reader038.vdocument.in/reader038/viewer/2022102906/56649ce35503460f949af248/html5/thumbnails/14.jpg)
What
To whom
Planned Year 3 Deliverables
Intended Impact
• Disseminate RosBREED deliverables and communicate with stakeholders through the use of published materials and participation in meetings
• Rosaceae breeders, allied scientists, industry
• Breeders: breed as efficiently as possible• Allied scientists: use newly generated science-based
knowledge, tools, databases• Industry:– interact effectively with local breeding
programs, understand power of DNA-informed breeding