oddrun anita gudbrandsen, phd, and aslaug drotningsvik, phd€¦ · • high cholesterol • fatty...
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Oddrun Anita Gudbrandsen, PhD, and Aslaug Drotningsvik, PhD
Dietary Protein Research Group, Department of Clinical Medicine, University of Bergen
Ola Flesland and Rune G. Madsen
TripleNine, Denmark
Fish proteins from blue whiting may promote health benefits in pets
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59.5% of cats and 55.8% of dogs in the US are classified as overweight or obese,
according to the Association for Pet Obesity Prevention (APOP)
That is approximately 56 million cats and 50 million dogs
Pets are getting fatter and sicker
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• Type 2 diabetes
• Reduced insulin sensitivity
• High blood pressure (hypertension)
• Coronary heart disease
• High cholesterol
• Fatty liver
• Kidney disease
Obesity increases the risk of developing
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• Type 2 diabetes
• Reduced insulin sensitivity
• High blood pressure (hypertension)
• Coronary heart disease
• High cholesterol
• Fatty liver
• Kidney disease
Obesity increases the risk of developing
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In humans, several (but not all!) studies show lower prevalence of obesity and obesity related
comorbidities in populations with high fish intake
Animal studies show beneficial effects of intake of fish (filet), fish oil and fish proteins on
diseases associated with obesity
Prevalence of obesity and comorbidities
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Develop obesity around 4 weeks of age
Develop high blood pressure, kidney damage, diabetes and high cholesterol with
increasing degree of obesity
Obese Zucker fa/fa rats
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Blood pressure development in Zucker rats
From: Luo 2015
100
110
120
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180
9 10 11 12 13 14
80
90
100
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9 10 11 12 13 14
Obese Zucker
Lean Zucker
Systolic blood pressure Diastolic blood pressure
Age, weeks Age, weeks
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No anesthesia necessary
Five measurements takes 6 min
Rats rest on a warming plate (37°C)
We measure diastolic and systolic
blood pressure and heart rate
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A small pelagic fish primarily used to produce fish meal for aquaculture feed
Its small size and discoloration limits the market for blue whiting as whole fish or fillet
for human consumption
In 2016, the world’s total catch of blue whiting amounted to 1.19 million tonnes.
Blue whiting consists 19-20% protein and has an average fat content of 5%
Blue whiting (Micromesistius poutassou)
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Fish was headed and gutted, added water and heated to 90°C
The cooked material was mechanically dewatered in a double-screw press to produce
stickwater (water-soluble fraction) and presscake (water-unsoluble fraction).
A whole meal was produced by combining the two fractions.
Hydrolysates were produced by enzymatic hydrolysis (alcalase and Protamex) of the whole meal
Blue whiting (Micromesistius poutassou)
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Blue whiting (Micromesistius poutassou)
Composition of the experimental diets 1
Contents (g/kg diet) Control diet BWW diet
Casein1 216.00 144.00
Blue Whiting Water-Soluble Fish Protein2
108.30
Cornstarch 511.67 475.50
Sucrose 90.00 90.00
Cellulose 50.00 50.00
Soybean Oil 70.00 70.00
t-Butylhydroquinone 0.015 0.015
Mineral Mix (AIN-93-MX) 35.00 35.00
Vitamin Mix (AIN-93-VX) 10.00 10.00
L-Methionine 1.60 1.60
L-Cystine 3.00 3.00
Choline Bitartrate3 2.50 2.50
Growth and maintenance supplement (#410751) 4
10.00 10.00
1 contains 92.5 % crude protein, 2 contains 61.5 % crude protein, 3 contains 41 % choline, 4 contains vitamin B12 2
(40 mg/kg) and vitamin K1 (25 mg/kg) mixed with sucrose (995 g/kg) and dextrose (5 g/kg). BWW: Blue 3
whiting water-soluble protein 4
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Blue whiting stickwater
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Blue whiting stickwater
Aim:
To investigate the effects of a diet containing water-soluble protein from blue whiting on
cholesterol metabolism in obese Zucker fa/fa rats
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Blue whiting stickwater
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Blue whiting stickwater
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Blue whiting stickwater
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• Reduce serum cholesterol concentration
• Reduce cholesterol amount in liver
• Mechanism of action: reduce the body’s own cholesterol production
Blue whiting stickwater: conclusions
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Aim:
Primary: To compare the effects of diets containing proteins from headed and gutted
blue whiting as whole meal or protein hydrolysates on the development of high blood pressure
in obese Zucker fa/fa rats.
Secondary: To investigate any changes in markers of kidney function, organ damage,
inflammation and oxidative stress, to examine the in vitro ACE and renin inhibiting properties of the blue whiting proteins.
Blue whiting presscake (not published yet)
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Background:
Fish proteins have been shown to inhibit angiotensin-converting enzyme (ACE) in vitro
Blue whiting presscake
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Prevented blood pressure increase by 20% in obese Zucker fa/fa rats
Effects on blood pressure did not correspond with in vitro ACE activity
Kidney function was not affected
No changes in inflammation and antioxidant stress
Blue whiting presscake: conclusions
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Aim:
Investigate if proteins from residual material and filet have different effects on
blood pressure development in obese Zucker fa/fa rats
Cod proteins
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The mean arterial pressure (MAP) was calculated as (diastolic blood pressure + 1/3 (systolic blood pressure - diastolic blood pressure)). SW-H; stickwater from Havstrand, PC-H; presscake meal from Havstrand, SWPC-H; stickwater + presscake meal from Havstrand, PC-G; presscake meal from Granit, FM-G; fillet meal from Granit.
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Figure 3. In vitro inhibition of activities of
angiotensin-converting enzyme (A)
and renin (B). Values are means, with
standard error of mean represented as
vertical bars. Values are shown for two
or three measurements for casein and
cod protein meals. Proteins are
compared using one-way ANOVA with
LSD post hoc test. Bars with different
letters are significantly different (p <
0.05). SW-H; stickwater from Havstrand,
PC-H; presscake meal from Havstrand,
SWPC-H; stickwater + presscake meal
from Havstrand, PC-G; presscake meal
from Granit, FM-G; fillet meal from
Granit.
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Presscake meal from cod residual materials with high gut content (PC-H) effectively
prevented the increases in MAP that are normally observed in obese Zucker fa/fa rats,
whereas the other cod meals tested did not influence the blood pressure development in
these rats.
In line with this, PC-H had the strongest effect on in vitro inhibition of ACE and renin
activities of the cod meals tested.
Cod proteins: conclusions
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Investigate effects of fish proteins on blood pressure and kidney function in dogs
Prevention vs treatment
Elucidate the mechanism(s) of action
Future perspectives
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Oddrun Anita Gudbrandsen: [email protected]
Ola Flesland: [email protected]
Rune Guldager Madsen: [email protected]
Contact