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Oka et al. supplementary Figure 1 Top DAPI Gr-1 MERGE ary Fig. 1. Identification of neutrophils by immunostaining with a Representative specimens of the UVB-irradiated skin of PLC +/+ mice Nuclei were visualized by DAPI staining. White bars, 200 m. Bars ins of the pictures denote the epidermis (blue) and dermis (red).

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Oka et al. supplementary Figure 1 Top. DAPI. Gr-1. MERGE. Supplementary Fig. 1. Identification of neutrophils by immunostaining with anti-Gr-1 antibody. Representative specimens of the UVB-irradiated skin of PLC  +/+ mice at 24 h - PowerPoint PPT Presentation

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Page 1: Oka et al. supplementary Figure 1     Top

Oka et al. supplementary Figure 1 Top

DAPI Gr-1 MERGE

Supplementary Fig. 1. Identification of neutrophils by immunostaining with anti-Gr-1 antibody. Representative specimens of the UVB-irradiated skin of PLC+/+ mice at 24 h are shown. Nuclei were visualized by DAPI staining. White bars, 200 m. Bars on the right margins of the pictures denote the epidermis (blue) and dermis (red).

Page 2: Oka et al. supplementary Figure 1     Top

Supplementary Fig. 2. Effect of UVB irradiation on the SA--gal activity in the skin. PLC+/+ and PLC-/- mice (n = 4 for each genotype) were irradiated with a single dose of 1 kJ/m2 UVB. Frozen skin specimens were prepared 24 h after the irradiation, and SA--gal activity was determined by staining SA--gal using a senescence detection kit (BioVision Research Products, Mountain View, CA) accordingto the manufacturer’s instructions. Representative photographs of the skin from PLC+/+ (+/+) and PLC-/- (-/-) mice are shown. Bar, 100 mm. Dotted lines denote epidermis.

Oka et al. supplementary Figure 2 Top

+/+ -/-