olav gaute hellesø university of tromsø norway¸_presentati… · waveguide propulsion of red...
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Optical trapping on waveguides
Olav Gaute Hellesø University of Tromsø
Norway
Outline
• Principles of waveguide propulsion
• Simulation of optical forces: Maxwell stress tensor vs. pressure
• Squeezing of red blood cells on a waveguide
• Trapping in a waveguide gap: Interference & levitation
• Phase-change due to trapped particle: S-parameters
• Summary
2
Waveguide propulsion
• Transparent microparticles are attracted by strong field gradient (evanescent field).
• Radiation pressure propels particles along the waveguide.
Fscat y
x
Fgra
d 200 nm
1.5 μm
Field distribution Top view of waveguide (Ta2O5)
3
Waveguide propulsion
• Transparent microparticles are attracted by strong field gradient (evanescent field).
• Radiation pressure propels particles along the waveguide.
z
x
Fgra
d
Fscat
200 nm
Propagating field Top view of waveguide (Ta2O5)
4
CCD camera
X-Y-Z Translation
Stage
Piezoelectric Controller
High N.A. 80X Objective lens
20X Long WD Objective Lens
Micro-chamber with cells
X-Y-Z Translation
Stage
40X Objective Lens Waveguide
Fiber Laser 5W@ 1070nm
Vaccum Suction
IR Filter
Waveguide propulsion– experimental setup
5
Waveguide propulsion – on straight waveguide
• Waveguides made of Ta2O5 (n = 2.1) • Red blood cells: 6 µm/s (in sucrose), Polystyrene microparticles: 50 µm/s,
Nanowires: > 500 µm/s !
Waveguide propulsion of red blood cells
Photon. Tech. Letters, 21, 1408, (2009)
Optics Express,18, 21053, (2010)
Optics Letters, 36, 3347–3349 (2011)
Waveguide propulsion of nanowires
6
y
x z
Simulation of optical forces with Comsol
• Define geometry
• Define mesh
• Set boundary conditions
• Find waveguide mode
• Propagate through geometry
• Find forces by integrating Maxwell’s Stress Tensor over the surface of the particle
• Memory-intensive, using e.g. 8x128 GB on a cluster
7
Simulation of optical forces with Comsol
• Forces by integrating Maxwell’s stress tensor (MST) over surface of particle:
– Comsol: emw.unTx, unTy & unTz
• Optical pressure given by field (ref. Brevik & Kluge, JOSA B, 1999): can also be integrated over surface to give force:
Fgrad
Fscat
s AM =1
4e0nw
2 nc
2
nw
2-1
æ
èç
ö
ø÷ Et
2 +nc
2
nw
2Er
2é
ëê
ù
ûú
a-
,
F = T ×n̂daSò , T ij =e
0er EiE j -
1
2dijE
2æ
èç
ö
ø÷+
1
m0
BiB j -1
2dijB
2æ
èç
ö
ø÷
Fx = s AMnx daS
ò
8
Simulation of optical forces with Comsol Force from MST vs. from optical pressure
n V/m
Zoom
Water
Thus:
• MST gives error for small index difference
• And for high???
Cause of error:
• Triangular mesh on spherical surface?
• MST subtracting large numbers?
• Error in Comsol for MST?
Polystyrene
9
Optical pressure
• Can be found from:
– The field using the expression of Brevik & Kluge, sAM
– Difference in diagonal radial components of MST on the two sides of a surface
kPa/W
Laser
Laser
10
Pressure (Pa) and direction a) From bottom, through w.g. b) Cross-section
Forces & pressure on red blood cells (RBC)
11 Analyst, 2015,140, 223-229
Force & torque on RBC
12 Analyst, 2015,140, 223-229
Squeezing of red blood cells on tapered waveguide
13 Analyst, 2015,140, 223-229
Loop with a gap: Transport & stable trapping
• On a waveguide, particles are continuously propelled forward
• Alternative: loop with intentional gap on the far side
• Particle is stably trapped in the gap by the counter-propagating fields
Laser
(a) (b)
Lab Chip, 12(18), 3436-40, 2012
Waveguide loop with a 2μm gap
14
Trapping in waveguide gap: Simulation of 2mm gap
• Symmetric about centre of
waveguide
Simulate half the problem
• Interference fringes caused by the
two counter-propagating beams
a) 3D-model
b) Field, side-view
c) Field, top-view
15 Lab Chip, 12(18), 3436-40, 2012
Trapping in waveguide gap: Simulation of 2mm gap
Horizontal and vertical
forces as sphere is
moved across gap:
Transversal and vertical
forces as sphere is
moved sideways out of
the gap:
16 Lab Chip, 12(18), 3436-40, 2012
Trapping in waveguide gap: Optical levitation?
Gap (10 μm)
Waveguide ends
Polystyrene sphere,
2 mm dia., fluorescent Water
Silica
Simulation frame
-1400
-1200
-1000
-800
-600
-400
-200
0
200
0,000 2,000 4,000 6,000 8,000 10,000
Vert
icalµf
orceµ(
fN)
Distanceµfromµwaveguideµendµ(µm)
-50
0
50
3,000 4,000 5,000 6,000 7,000
(a)
-20
-10
0
10
20
30
40
50
60
0,000 0,500 1,000 1,500 2,000 2,500 3,000 3,500 4,000Vert
ical
forc
e (fN
)Vertical position (µm)
(c)
• 2mm gap = tight trapping
• 10mm gap = levitation?
Positive values
= pushing up!
17 Optics Express, 6601-6612 (2015)
Trapping in waveguide gap: Optical levitation!
0 5 10 15 20 25−10
0
10
0 5 10 15 20 25−5
0
5
Rel
ativ
eve
rtica
ldis
plac
men
t(µm
)
Time(s)
2.17μm
Laserµoff
zµ=µ5.98μm
Rel
ativ
eµz-
posi
tionµ
(μm
)
Rel
ativ
eµve
rtica
lµpos
itionµ(μ
m)
z
In gap On waveguide Laser off, leaves gap
x
18 Optics Express, 6601-6612 (2015)
On-chip phase measurement
19
Measurement set-up:
Waveguide Young interferometer
Simulation using PMLs, input and
output ports.
Phase and transmission found from
S-parameters. Lab Chip, 2015,15, 3918-3924
On-chip phase measurement
20
Measured Simulated
Resonances!
(not measured)
Lab Chip, 2015,15, 3918-3924
Summary
• Comsol works fine to find the field around nano- and microparticles on waveguides – But memory-hungry: Up to 1TB RAM
• Optical forces: Problem with Maxwell’s stress tensor • Optical pressure: Can be combined with mechanical model? • Interference and resonances require tight sampling • Critical to avoid reflection from PML at end of waveguide • Use PML with slit port excitation for counter-propagating
beams
21
Thanks to:
• Balpreet Singh Ahluwalia, Per Jakobsen, Pål Løvhaugen, Firehun Tsige Dullo and Øystein Helle
• Waveguide fabrication at the Optoelectronics Research Centre, Southampton, UK: James Wilkinson and Ananth Subramanian (now Ghent)
• Cell work: Thomas Huser (Bielefeld), Ana Oteiza and Peter McCourt (UiT)
• Funding: Research Council of Norway
• Computer resources: Notur - The Norwegian metacenter for computational science
22
Squeezing of red blood cells on narrow waveguide
Squeezed
Cell
Laser on Laser off
Waveguide
(a) (b)
• RBCs are squeezing on waveguides < 6µm wide
• RBC regains shape when laser is switched-off
• No permanent loss of RBC elasticity was observed
3 µm ~3 µm
~6 µm
Laser On
Laser Off
(a)
(b)
Waveguide
23