2.

Eg to CD4 inside the cell blocks CD4 expression at the cell surfacevpu aid nef slnc products uprcgulate CD4 and MHC Class I

C. Syncytia formation kills both infected and uninfected cellsD. Increase in plasma membrane permeability results in the uptake of lethal amounts

of calcium (osmotic lysis)E. vRNA and unintegrated vDNA accumulation is toxic to cells

select the INCORRECT statement concerning Type I Immediate (Anaphylactic)Hypersensitivify.

A. Antigen binds to IgE on the surface of mast cells causing the release ofinflammatory mediators.

ires the formation of IgE antibody.ipg specific IgE that is firmly artached to lrceRl on mast

or activated eosinophilsRe-exposure to the same antigen results in cross-linking of the cell-bound IgE andrelease of inflammatory mediators within minutes.Crosslinking of FceRI on the surface of mast cells by antigen and IgE causesmast-cell activation and degranulation.

3. Select the INCORRECT statement concernins Autoimmune Diseases.

MMI4804. - 2004Quiz 5

1. Select the INCORRECT statement about HIV cytopathic effects.

A.

B.C.D.

5.

D.

E.

Autoimmune diseases are chronic inflammatory responses directed againstcomponents of the body.Immunity against self antigens (called autoantigens) is called autoimmunity.They are much more prevalent in females.2-3% of the population of developed countries suffers from an autoimmune

+.

6.

cttrbIe.fypcihypcrsensit iv i tyrcact ions.

Some tumo$ secrete immunosuppressive molecules causing the immune response to be

down-regulated. TRUE

In Myasthenia Gravis, antibodies bind to the acetylcholine receptor on muscle cells

resulting in their endocytosis and degradation. TRUE

Rabies fmmune Globulin EiC) is used for prevention of rabies for those exposed to the

virus and is produced by bleeding feral rabid dogs, squirrels and horses that have bitten

humans- FALSE

7 ' The phenomenon in which further immunogrobulin rearrangement is shut down onthe other chromosomal allele is called

A. Clonal selectionB. Allelic imprinting

1e)ettetic exitusionY. Isotype switching

E. Productive rearrangement

8' The process by which neutuophils cross the vascular endothelium is called

LA-2diapedesis ," .;B. chemotaxisC. complement fixationD. opsonizationE. polymorphonuclear leukocyte response

9' which of the following is not a characteristic of macrophages?

A. secretion of soluble mediatorsI kill ing tumor cells

€) rearrangeme nt of anti gen-speci fi c receptorsD. phagocytosis of immune complexesE. presentation of antigen to T cells

I0' which combination of cytokines act in the chemotaxis of polymorphonuclearleukocytes (pMNs)?

A. IL-2 and IL-4

@ IL-$ and llrlFcrC. L-10 and IL-12D. IL-4 and IL-5E. none ofthe above

1 l. Membrane-bound IgM characterizes which of the following cell types?

A. T helper cellB. plasrna cell

(Q i^-unocompetent B cellY cvtotoxic T cellE. dendritic cell

12. Which of the following would be a CD4 positive cell?

A. plasma cellB. cvtotoxic T cell

$ thelper cetlD. langerhans cellE. macrophage

13. Which is the order of a pathogen that crosses the epithelial barrier in highconcenhation?

@ connective tissue > peripheral lymphatics > lymph node > vasculature > spleenB. connective tissue > peripheral lymphatics > spleenC. vasculature > spleen > peripheral lymphatics > lymph nodeD. connective tissue > peripheral lymphatics > lymph node > thymus > vasculature >

spleenE. vasculature > spleen > lymph node

*d 14. Which of the following best describes the differences between how T cells and Bcells recognize antigen?

A. T cells recognize self MHC molecules, while B cells recognize soluble antigen.

Q f ce[s rccognize foreign peptides in the context of self MHC molecules, while Bcells recognize native, foreign proteins.

C. T cells rccognize protein antigens, while B cells recognize non-protein antigens.D. T cells recognize foreign peptides, while B cells recognize whole proteins.E. T cells recognize self MHC molecules, while B cells recognize peptide antigens.

15. Peptide loading of MHC class II molecules with foreign antigen occurs

- {\r.- @)in endosomal/lysosomal compartmentB. in cytosolC. on cell surfaceD. in endoplasmic reticulumE. in golgi

16. T cell development in the thymus includes the following processes:

A. T cell receptor rearrangement :

B. negative selectionq positive selection

*@ all of the aboveE. only B and C

' . - ' 17. After degradation in the cytoplasm, fragments of foreign proteins (ie virus-derived)are pumped into the endoplasmic reticulum for MHC class I peptide loading withhelp of..-.

A. invariant chainQ proteasome complex

I €)Transporter Associated with Antigen Processing (TAp)D. MHC class IIE. None of the above

l8.Susceptibi l i tytoautoimmunediseaseisinfluencedby-.

A. genetic factorsB. environmental factors

(!) botU A and BD. none of the above

' '"' 19' Which of the following autoimmune diseases is characterize<l by the presence ofimmune complexes formed befween antibodies and DNA, histone, and ribosomalantigens?

;\ rheumatoid arthritis

@ systemic lupus erythematosus (SLE)C. Hashimoto's diseaseD. multiple sclerosisE. none ofthe above

20' T cell autoimmunity against myelin antigens can lead to which autoimmune disease?

multiple sclerosismyasthenia gravisinsulin-dependent diabetes mellitus (IDDM)

D. rheumatoid arthritisE. none ofthe above

2l ' Microbial infections can precipitate autoimmune diseases in what way(s)?

\!J molecular mimicryB. pathogen-induced tissue darhage .

^A release of autoantigens

<--€lall of the aboveE. none ofthe above

t' 22' Mechanisms of peripheral tolerance include all of the following EXCEpT 2

A. Activation induced cell deathB. CTIA-4 signaling

L. Production of suppressive cytokines by regulatory cells\D) Destruction of autoreactive cells bv CTLs

E. Clonal anergy

23. Which of the following is NOT a function of T helper I (Thl) cells?

A. Production of IFNyB. Activation of natural killer (NK) cells vialL-2 production"C. Promotion of cytotoxic T cell differentiation

@ pro-otion of IgE production by B cells , r..:E. Activation of macrophages and dendritic cells.

24. Which of the following costimulatory molecules bind to 87 on antigen presentingcells?

A. CD28B. CTLA4 ,;I ".

( 'r

c. cD40D. all of the above

@>both A and B

,,. 25. Which of the following statements is TRUE regarding narve T cells?

A. Naive T cells circulate throughout the entire body, including the spleen, andreadily leave the intravascular space.

B. NarVe T cells produce large amounts of effector cytokines such as IFNy and IL- t ,

t2.Naive T cells express T cell receptor but have not been exposed to antigen.

D. Naive T cells are extremely short lived, with an average survival time of 8-12hours.

E. Naive T cells require little costimulation for activation..

26. Which of the following statements are TRUE regarding memory T cells?

A. Memory T cells have an extremely long life span.B. Memory T cells are derived from the population of effector T cells'C. Ivtemory T cells require little costimulation for proliferation and cytokine

production.D. Memory T cells circulate through the entire body and readily leave the

- intravascular space.

@eu of the above

27. Which of the following describe the role of CD28 in T cell activarion?

A. CD28 on the T cell binds to CD4OI, on the antigen presenting cell.B. Activation of CD28 promotes cell death.C. Preferential engagement of CTLA-4 versus CD28 may lead to cell proliferation.D. Only A and B are correctp. A, B, and C are correct

fF\, None of the above '.u , jL-,/

. 28. The bone marrow is an imoortant site for

' - ' ' . .^\r -j-.!&) hematopoiesis1 B. B cell maturation

C. T cell maturationD. immune surveillanceE. central tolerance

- ,, 29. Which of the following does not protect the external body surfaces?

A. mucous.'B. skin

:-l-9. gutttic acid-Xal salivary.amylase

E. gut microflora

30. The process by rvhich complement enhances phagocytosis of bacteria is called

,^\' 0.) opsoruzatron.K-chemotaxisC. exocytosisD. proteolysisE. bacteriolysis

3l ' which one of these conditions is not directry associated with HIV?

Profound immunosuppressionOpportunistic infections and CNS degenerationMultiple sclerosisMalignancies and wasting

32. Which statement is incorrect about CCR5?

A Is expressed on dendritic cells, macrophages, CD4+ T cells 1 ,@ Appears late in disease and is the onlt'coreceptor for HIV

r' r* r

9. ls the receptor used by the most frequently transmitted virus (mucosal route)D. Serves as coreceptor for macrophage-tropic HIV strains

33. Which statement is incorrect about HIV pathogenesis?

vpu and nef gene products upregulate CD4Syncytia formation kills both infected and uninfected cellsHIV specific crl- attack cause an increase in plasma membrane permeabilityresults in the uptake of lethal amounts of calcium (osmotic lysis of infected cell)vRNA and unintegrated vDNA accumulation is toxic to cells

34. Which of the following is not an advantage of killed vaccines?

A. Cannot revert to virulenceB. More heat stable

. c. can be safely given to immunodeficient patients and pregnant women'B L induces a weaker mucosal IgA antibody response

35. Which statement is not true about Subunit Viral Vaccines?

A. Contain primarily the immunity-inducing component of the virionB. Consist of protein or portions of proteins from virus particles

AContain active nucleic acid consiiting of the entire genomeH. Can be natural or recombinant proteins

A.B.

I

qc.

D.

36. Which statement is not accurate about Bacille Calmette-Guerin (BCG) Vaccine?

.A It is a live attenuated strainof Mycobacterium &ovls ftovine strain)(U)Is routinely given in Europe, US and other parts of the world rn.' ' r" " rd' ,.r . 'rT. It invalidates the TB skin test used to verify exposure to the tubercle bacillus

D. Is effective against human tuberculosis (TB).,

37. which statement is not true concerning Type I Immediate (Anaphylactic)Hypersensitivity?

A. First exposure to antigen stimulates production of IgE that bind to the surface of

- mast cells

(8lB'l Results in formation of IgE antibody that is not antigen specificC. Antigen binds to antigen specific IgE that is firmly attached to FceRI on mast

cells, basophils and activated eosinophilsD. Re*exposure to the same antigen cross-links the cell-bound IgE causing release of

inflammatory mediators within minutes (immediate reaction)

38. Which statement is not accurate conceming atopy or allergy?_,. - , . " i

r , . '

Less than l0% of people in the US have experienced an atopic disorder

D. Genetic predisposition includes mutations in the genes encoding the alpha chainof IL-4 that enhances the effectiveness of IL-4, resulting in increased amount ofIgE synthesis by B cells

39. Which statement is incorrect conceming Type IV Delayed-Type Hypersensitivity(DrH)?

A. It is a function of T lymphocytes and can be transferred by sensitized Thl cells(CDt T ceils)

B.' Response is delayed and begins hours or days (peaks at24-48 hours) afterexposure to antigen..

Q There is a slow inflammation at the site of antigen exposue .'fD) fne tuberculin test is always positive in people with suppressed cell-mediated

rmmunity (AIDS, measles, lymphoma) .f,

40. which statement is not accurate concerning tumor suppressor genes?

A. They encode proteins that prevent the unwanted proliferation of mutant cellsB. p53 is a fypical tumor suppressor gene and is muiated in 50% of human cancer

casesC, They are expressed in response to DNA damage

pnsl gene encodes a protein that causes the damaged cell to proliferate

41. Which one of these viruses is not associated with induction of human cancer?

A. Human Hepatitis B Virus and Hepatitis C VirusB. Human Papillomavirus VirusC. Epstein-Barr Virus

@lnnu"nza Virus

42. which statement is not true of the immune response to T and B lymphomas?

A. The T cell receptor expressed by a T cell lymphoma can be a tumor specificantigen.

B. The membrane immunoglobulin expressed by a B cell lymphoma can be a tumor

^specific antigen.

(p|ryan papillomavirus virus (F{PV) can be a rumor specific antigen-D. Both cytotoxic T lymphocytes (CTLs) and antibodies can r"cog.rize tumorspecific antigens

43. which of the following.tests can not be used to measure an antigen level?

A. immunodif fusion . . - . i ̂. i : . . , , , . ! '4

(E complement fxationE Ertsa I t : : r ' ' - r " : -"^4{4

D. Western blot "

, , ;$. i , ;g ; , . r : ( l ' i

44. A 3 year old presents with oral ulcers, poor dentition, and a gluteal abscess whichcultures S. aureus. You suspect he has a primary neutrophil defect. Considerationsfor diagnosis include all of the following except .----.-.

A. Job's syndromeB. leukocyte immunodeficiency syndrome (LAD-1)

tC)iGeorse svndrome .\ - - l^ 'D. Chronic granulomatous disease (CGD)

45. Which of the following patients with recurrent infections is most likely to survive toadulthood?

A. severe combined immunodeficiency syndrome (SCID)9.. Wiscott-AldrichsyndromerR\tC.) common variable immunodeficiency syndrome (CVID)D. ataxia telansiectasia

46. T cell number olr.,n"tion can be measured by all of the following except

A. flow c5rtometryB. delayed type hypersensitivity (DTH) responseC. lymphoproliferationD. cytokine nroduction

@ ainyarorhodamine oxidation

**** ' t : lc***+******x*{<**+*******,F**************,k**{<***t<***:r** i<*****t **{<trx

For each of the following questions, please answer A if the statement is true and B if thestatement is hlse.

In the acquired immune response, B and r lymphocytes only generate receptorspecificitiesJhat bind to previously encountered foreign antigens.

Each imrrunoglobulin molecule has 2 antigen binding sites that are each made from4 polypeptide chains.

Mature T lymphocytes oeanng a particular T cell receptor will undergo somatichypermutation and affinity maturation upon encountering foreign antigen (presentedon self-MHC on antigen oresenting cells) in the periphery.

Naturat kilter (NK) celts uan kill turqor cells in the absence of MHC class I.

IL-2 can induce both apoptosis and prourbration of T cells.

47.

48.

49.

50.

51.

52. Transplant rejection occurs when a transplanted donor organ is recognized as foreignand is attacked by the recipjqnt's immune system.

53. Immunoglobulin V heavy chain gene recombination is similar to T cell receptor Bchain recombination becausp they both use diversity (D) gene segments to make VDJgene segments.

54. The gene segment Ce encodes thg constant region of the IgD isotype.

55. The strongest genetic factor inl^*--cing susceptibility to autoimmune disease isinheritance of particular HLA allqles.

56. Inappropriate regulation of T helper cell responses likely plays a role in a majority ofautoimmune diseases.

57. Selective IgA deficiency is the most common inherited immunodeficiency in the USpopulation.

58. Detection of antigen specific antibodies of isofype IgM is routinely performed inclinical laboratories.

59. Cells destined to become B cells arise trom hematopoietic stem cells in the bonemarrow, enter the circulation, and are directed to the thymus by chemotactic factors.

60. Recurrent bacterial infections are alwayqpredictive of inherited or acquiredimmunodefi ciencv svndromes.

I

I

MMI480A 2002 Final Exam

l.A2.B3.D4.A5.C6.87.C8.A9.C10. Bl l .c12.c13. A14. B15. A16. D17. C18. c19. B20. A2t.D22-D23. D24-E2s.c26.E27.F28. A29.D30. A

31. C32.833. A34.D35. C36. B37.838. A39. D40. D41. D42.C43.844. C4s. c46.847.848. B49.850. A51. A52. A53. A54. B55. A56. A51. A58. B59. B60. B

ffir\

MMI4SOASpring 2002

Final Examination

For each of the following question, select the single best answer to fili in on your answer

sheet.

l. Which of the following are true statements conceming the differences between

antigens and immunogens?

,r'i:*-\

( 1,. )ntigens are targets of an immune lesponse, while immunogens induce an

une response'-8. Immunogens induce cellular immunity, while antigens induce humoral immunity.

C. In order to induce an immune response, immunogens need to be coupled to a

foreign protein "carrier", while antigens can be injected alone'

D. All of the above are true statcmentsE. Only A and B are correct

2. The membrane protein on an antigen presenting cell which is used to bind and present

antigen to helper T cells is --.

T cell receptorClass II

MHCIgACD4

Class I

3. Which of the following are true statements concerning clonal selection?

A. Each lymphocyte generates one unique antigen-binding specificity

B. Self-reactive lymphocytes are deleted

, C* Foreign-reactive lymphocytes proliferate and differentiate

(y) All of dre above are true statementsE. Only A and B are correct

4. Which of the following are true statements concerning immunoglobulin isotypes?

( '1' \A--The isotype of an immunoglobulin is determined by the type of heavy chain that it

contains.B. Isotype switching refers to a change in the antigen specificity of each isotype.C. The isotype of an immunoglobulin is determined by the light chain gene locus

(either r or L).D. AU of the above are true statementsE. Only A and B are correct

5. The effector molecule of the humoral immune system which is transported acrossepithelia and into secretory products such as saliva is

A. IgM_--8. CD8

f,e) lgeY invariant chain

E. IgE

6. Which of the foliowins best describes the differences between recombination of"' immunoglobulin heavy u"'a figfrt chain genes?

A. Heavy chain genes rearrange DNA sequences, while light chain rearrangement is-*--: _ performed by RNA processing.

€) Heavy chain genes encode V, D, and J segments that rearrange, while light chainv rearrangement uses only V and J gene segments.

C. Heavy chain genes undergo rearrangement after light chain genes.D. Heavy chain, but not light chain gene rearrangement uses mechanisms of

junctional diversity.E. The heavy chain genes are encoded on two different chomosomes, while light

chain genes are encoded on one.

i ' immuno quiz 1+ answers,2002

\

Strona I z

1. True/False The acquired immune response is more essential than the innate immune response in thedefense against microbes.

2.Which of the following best describes the differences between how T cells and B cells recognizeantigen?

A. T cells recognize self MHC molecules, while B cells recognize soluble antigen.B. T cells recognize foreign peptides in the context of self MHC molecules, while B cells

recognize soluble antigen.C. T cells recognize protein antigens, while B cells recognize non-protein antigens.D. T cells recognize foreign peptides, while B cells recognize whole proteins.E. T cells recognize self MHC molecules, while B cells recognize peptide antigens.

3. Which of the following are true statements concerning the generation of diversity at theimmunoglobulin

l:uuY chain locus

A. Somatic hypermutation occurs before a B cell is exposed to antigenB. lsotype switching occurs before a B cell is exposed to antigenC. P- and N-nucleotides are added to D-J gene junctions before a B cell is exposed to antigenD. All of the above are true statementsE. Only A and B are correct

4. True/False Each antigen binding site of an immunoglobulin molecule is made up of 6 hypervariableloops.

Quiz l with answers

S.The process by which

A. phagocytosisB. chemotaxis

neutrophils cross the vascular endothelium is called

C. complement fixationD. diapedesisE. acute phase response

6. Interferons are induced in a viral infection by the presence of

A. single stranded RNAB. single stranded DNAC. double stranded RNAD. All of the aboveE. None of the above

http ://medocs.ucdavis. edu/muril.t8 0a/exams I A2-quizIYo2B ansrvers.htm 2005-05-30