oral talk in 'paa annual meeting 2012
TRANSCRIPT
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Resistance of potato genotypes to drought stress
Pankaj Banik, M.Sc. Student
University of Saskatchewan, Canada
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Drought stress in potato plants
• Sensitive to drought
– Even a short period of time
• Tuber quality & production
• Stolon formation & tuberization
– Most sensitive stage
• Longer period in tuber formation
• Decrease in tuber number, growth & yield
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Greenhouse Low RH room
(22/20°C, 17-25% RH, 250 µmol/m2/sec)
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Applying drought stress
0
5
10
15
20
25
30
35
So
il m
c%
1st DA 2nd DA 1st DS 2nd DS 1st R 2nd R
Drought acclimated & stressed (DAS)
Non acclimated but stressed (NAS)
Non acclimated & non stressed (NA)
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Stress & Recovery
Severe stress (scale 3)
After recovery (scale 0)
Score % leaves wilted
0 None
1 25%
2 50%
3 75%
4 100%
5 Plant died
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Measurements
• Leaf WC
• Stomatal aperture
• Water Use Efficiency
• Leaf water loss over 15 minutes
• Leaf temperature
• Leaflet water content
• Flowering time
• Stem diameter and plant height
• Biomass
• Trichome Number
• Leaf cuticle layer
• Yield
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Hypotheses
• Drought stress history of genotypes affects
subsequent drought stress resistance [V1002-2]
• Drought acclimation and recovery are key
components of resistance
[Fv12246-6, V1002-2 & Russet Burbank (RB)]
• Stomatal aperture parameters are associated with
yield under drought stress conditions
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Drought stress History over 3 generations
Generation 1 NA
(Non Acclimated)
DA
(Drought Acclimated)
Generation 2 NA-NA NA-DAS NA-NAS DA-NA DA-DAS DA-NAS
Generation 3 NA-NA-NA NA-DAS-NA NA-NAS-NA DA-NA-NA DA-DAS-NA DA-NAS-NA
NA-NA-DAS NA-DAS-DAS DA-DAS-DAS
NA-NA-NAS NA-DAS-NAS DA-DAS-NAS
}
}
}
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Previous Drought Acclimation History induced
higher tuber numbers in 5-50g wgt classes
0 2 4 6 8 10 12 14 16
NA-NA
NA-DAS
NA-NAS
DA-NA
DA-DAS
DA-NAS
Tre
atm
en
ts
Tuber No.
<5g
5-20g
20-50g
50-100g
>100g
<5g 5-20g 20-50g 50-100g >100g
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Treatment with No Acclimation History had
lowest leaf water content at maximum stress
74
76
78
80
82
84
86
88
NA-NA-NA NA-NA-DAS NA-NA-NAS NA-DAS-NA NA-DAS-DAS NA-DAS-NAS DA-DAS-NA DA-DAS-DAS DA-DAS-NAS
Treatments
% le
af
wa
ter
co
nte
nt
Overall difference among treatments: ***
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Recovery Stage:
Drought Acclimation History either in Generation 3 alone
or with combined Generations 1 + 2 induced lower rate of leaf
water loss compared to NA-NA-NA and NA-DAS-NA
0
5
10
15
20
25
30
35
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Time (min)
% w
ate
r lo
ss
NA-NA-NA
NA-NA-DAS
NA-DAS-NA
NA-DAS-DAS
DA-DAS-NA
DA-DAS-DAS
A
B
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Difference in leaf cuticle layer morphology over 3 successive generations
NA-NA-NA
(no stress history)
Stomata
DA-DAS-DAS
(Stressed over 3
generations)
Φ = 0.1mm2, 600X
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Hypotheses
• Drought stress history of genotypes affects
subsequent drought stress resistance
• Drought acclimation and recovery are key
components of resistance
{Fv12246-6, V1002-2 & Russet Burbank (RB)}
• Stomatal aperture parameters are associated with
yield under drought stress conditions
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Experiment layout (no stress history)
Genotypes Treatments Codes
Fv12
NA (Non Acclimated) Fv-NA
DAS (Drought Acclimated & Stressed) Fv-DAS
NAS (Non Acclimated & Stressed) Fv-NAS
V1002
NA V-NA
DAS V-DAS
NAS V-NAS
RB
NA RB-NA
DAS RB-DAS
NAS RB-NAS
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Drought Acclimation did not increase total % leaf water
content at maximum stress.
74
76
78
80
82
84
86
88
90
Fv NA Fv DAS Fv NAS V NA V DAS V NAS RB NA RB DAS RB NAS
Treatments
% l
eaf
wate
r co
nte
nt
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HOWEVER, rate of water loss was affected.
Non-Acclimated (NA) treatments had highest rate of % leaf water loss.
Overall, V1002 lost water at a significantly higher rate than Fv12 & RB.
0
1
2
3
4
5
6
7
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15
Time (min)
% w
ate
r lo
ss
Fv-NA
Fv-DAS
Fv-NAS
V-NA
V-DAS
V-NAS
RB-NA
RB DAS
RB NAS
NA
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Drought Acclimation had the most significant
impact in RB shoot-related responses.
0
2
4
6
8
10
12
14
16
Fv NA Fv DAS Fv NAS V NA V DAS V NAS RB NA RB DAS RB NAS
Final stem diameter
0
1
2
3
4
5
6
7
Fv NA Fv DAS Fv NAS V NA V DAS V NAS RB NA RB DAS RB NAS
Final stem No.
0
20
40
60
80
100
120
140
160
180
Fv NA Fv DAS Fv NAS V NA V DAS V NAS RB NA RB DAS RB NAS
Final shoot dry wt
0
50
100
150
200
250
Fv NA Fv DAS Fv NAS V NA V DAS V NAS RB NA RB DAS RB NAS
Final plant height
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Summary
• Previous stress history • Induced more tubers in the 5 – 50 g size classes.
• Drought Acclimation
» Increased Leaf Water Content (LWC) under max. stress
» Reduced rate of % leaf water loss
» Difference in leaf cuticle layer
• No previous history • Drought Acclimation
Did not increase LWC
Did reduce rate of % leaf water loss
Had most significant effect on RB shoot responses
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Next Experiments
• Stomatal aperture evaluation on NA, DAS
and NAS in drought history and no previous
drought stress history experiments.
• Water Use Efficiency, non-destructive Water
Potential measurements
• Specific response measurement in all six
genotypes received from Dr. Bizimungu.
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Acknowledgements
Prof. Karen Tanino, University of Saskatchewan (U of S), Canada
Dr. Benoit Bizimungu, AAFC, Fredericton, NB.
Dr. Helen Tai, AAFC, Fredericton, NB.
Prasanto Mondol, PhD student, U of S
Prof. Sakti Jana, U of S
Eldon Siemens, U of S
Jackie Bantle, U of S
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Thank you