organelle structure and function · organelle structure and function! organelles! molecules!...
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Organelle Structure and function
Organelles Molecules Cellular function
Ch 5: Cells, the working units of life Ch 27: The origin and diversification of Eukaryotes
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Discussion Summary: Week 2 • Cell Biology’s Open Cases • Team Discussion: • Related topics • Questions • Take home message
• Group discussion • Type the take-home messages in D2L
or write on board • Write questions on the board
• Brief review of “relevant facts” deemed important by instructor.
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“Organelle Structure and Function”
Re-cast this idea as:
Cellular Function(s) Molecular basis
Organelles’ role(s) In terms of organismal/evolutionary study…
Structures and traits
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Prokaryotic cell features Review of form,
molecules and function h"p://www.sigmaaldrich.com/technical-‐documents/ar7cles/biology/glycobiology/pep7doglycans.html
h"p://bio1151.nicerweb.com/Locked/media/ch27/capsule.html
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Prokaryotic cell features Review of form,
molecules and function h"p://www.bmb.leeds.ac.uk/illingworth/6form/
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Eukaryotic Cells: Classic and EM views FUNCTION relates to STRUCTURE
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Studying Organelles:
Physical and
chemical properties
Isolate
associated molecules
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Highlights: Endomembrane
system • Membrane-
associated protein production
• Organelles, molecules, FUNCTIONS • Lipid
synthesis • Protein
modification
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Special Plant Organelles
• Unique chemistry
• Important functions
• Plastids • Glyoxysome • Which one
not on this slide?
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Unsung heroes! The “other” organelles and
structures • Peroxisome • Many special
reactions here • Catalase breaking
down H2O2 quite important!
h"p://flipper.diff.org/app/items/3693
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Unsung heroes! The “other”
organelles and structures
• Extracellular Structures • How do
molecule get outside the cell?
• How are they assembled into structures?
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Unsung heroes! The “other” organelles and structures • Cytoskeleton • So many types! • What does this mean about function?
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Unsung heroes! The “other”
organelles and structures
• Cytoskeleton • How can we
study function?
• Hypothesis-driven expeiment
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Protists • Insight into evolution • Organelles • O2, ATP, Membranes
• Multicellularity • Single-celled organisms
useful for investigation • Diversity sparks
question about function
• Ecological impact
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Protists • Insight into evolution • Organelles • O2, ATP, Membranes
• Multicellularity
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Protists
• Single-celled organisms useful for investigation • Use of yeast • Use of chemistry • Visualization of
molecules and chemical properties
• “elegant” experiment!
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Protists: MCB and OEB • Diversity sparks
questions about function • Classical TRAITS are
cellular • Classification • Evolution • Phylogenies
• MOLECULAR mechanisms underlie structure and function • Biochemistry • DNA
• SEXUAL REPRODUCTION leads to diversity
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Protists’ interaction with
environment • Ecological
impact • Geological • Biochemical • Symbiosis • Disease • Mutualism • Etc.
h"p://www.treadright.org/project/white-‐cliffs-‐dover h"p://earthobservatory.nasa.gov/Features/Phytoplankton/page2.php
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Protists’ interaction with
environment • Ecological
impact • Geological • Biochemical • Symbiosis • Disease • Mutualism • Etc.
h"p://www.treadright.org/project/white-‐cliffs-‐dover h"p://earthobservatory.nasa.gov/Features/Phytoplankton/page2.php
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STRETCH BREAK!
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Investigating Life : Chapter
1 • Figure 1.12 • Gather related
and necessary “facts” to set up and understand experiment (Fig 1.11)
• Discuss data prediction (deductive reasoning)
• Discuss actual data and conclusions
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Investigating Life : Chapter 1 • Figure 1.11 needed as
reference for 1.12 • Observations during first
experiment help form hypothesis about next experiments
• Come up with ideas about how things work (inductive reasoning … conclusions from observations or experiment)
• Note experiment types: Controlled or comparative
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This week’s lab: Lab 1b, 1c intro
How was pre-lab? Planning your preparations and views
Some questions Some predictions Use last week’s data!
Using the digital camera Logging your images Fluorescence and Phase-Contrast microscopy Questions??
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REVIEW of
PRE-LAB requirements
PROCEDURE: • Obtain lab(s) from course website • Complete PRE-LAB - Summary section - Detailed method, data section - Questions
• Create digital version of PRE-LAB - Upload to D2L, group area by 8am
Tuesday • Review and assess partner’s: - Tuesday afternoon meeting
recommended - Upload RUBRIC to D2L
- Edited PRE-LAB secured in lab notebook by 8am Thursday. - Incorporate coments from partner.
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Next week’s lab: Lab 2 : Protein quantitation. Introduction
• 4 major molecules in biology • Spectrophotometry, standard curve,
dilutions, use of calculations • Proteins have numerous functions in cells,
and measuring protein is part of molecular biology and/or biochemistry
• How much protein per serving in a “supplement” ?
h"p://www.di.uq.edu.au/sparqspectro
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Next week’s lab: Lab 2 intro ��� • Future labs will use these techniques
• Dilutions create varying concentrations of standard (BSA), and measurable amounts of unknown.
• Bradford assay is Coomassie Blue & Spectrophotometry
h"p://www.qcbio.com/pierce/23236.htm
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Next week’s lab: Lab 2 intro ��� • Future labs will use these techniques
• Bradford assay is Coomassie Blue & Spectrophotometry
h"p://www.di.uq.edu.au/sparqspectro