organic photochemistry - college of william & mary · 2020-06-13 · organic photochemistry...
TRANSCRIPT
Organic Photochemistry
with the
Harbron Group
Photochemists use light…
• to do chemistry
• to interrogate molecules
• or to do both
AlUV B
pre-irrad.
post-UV irrad.
post-VIS irrad.
Our Focus
• Stimulus-responsive fluorescence
FL+ stimulus
- stimulus
FL
FL FL
FL FL
Fluorescence properties make it particularly
well-suited to sensing and imaging
• sensitivity
• many observables
– intensity
– wavelength (color)
– lifetime
– anisotropy
• spatiotemporal resolution
Current Project Areas
• Light
– fluorescence intensity modulation
induced by visible light
– “release and report” system for
simultaneous photocontrol of analyte
and fluorescence detection
• Mercury
– mercury ion sensing in water with doped
conjugated polymer nanoparticles
• Green Fluorescent Protein
– new GFP-dye analyte sensors
Conjugated Polymer Nanoparticles
• CPNs (Pdots) can be used as the fluorophore in
stimulus-responsive fluorophore-dye constructs
– Stably suspended in water
– Covalent or non-covalent dye incorporation
– Photophysical advantages
+stimulus
FRET
Fluorescence Photomodulation with lvis
• Goal: off-to-on photoswitching with visible light
• Application: super-resolution microscopy (2014 Nobel Prize)
Photorelease & Report
• Goal: achieve photocontrol of analyte with simultaneous
fluorescence readout
• Application: biological imaging experiments
Mercury Detection
• Goal: sensitive, selective, ratiometric detection of
mercury ions in water
• Application: environmental testing, imaging
Hg2+
FRET
- HgS
-Hg
CPNs fluoresce
RB-SL “silent”
+Hg
Hg promotes RB-Sl RB-Hg
CPNRB-Hg FRET activated
CPN and RB-Hg fluoresce
GFP-based Sensors
• Goal: develop new fluorescent sensors based on GFP-
dye conjugates
• Application: biological imaging
image credit: Zeiss Microscopy Online Campus at FSU National
High Field Magnet Lab
Harbron Research Lab
+ ?
Matt McCarron MS ‘18, Lisa Graves MS ’19, Tyler
Larson ‘18, Hank Hannon ‘19, Andrew Dahik ‘19,
Elizabeth Amato ‘18
You?
Lab Life
Measuring
Communicating
Making
Fun
Making
• Organic synthesis
• Purification (chromatography)
• Characterization (NMR)
• Nanoparticle preparation
Measuring
• Absorption and fluorescence
• Response to stimulus (we have lights! lasers!)
Communicating
• Group meeting
• Undergraduate research symposium
#labhood