1

ORIGINAL GEL IMAGES for Figure 4

Figure 4d – Actin (42 kDa)

Figure 4d – ATF4 and cleaved ATF6 (49 kDa and 55 kDa)

Figure 4d – Caspase-3 (19/17 kDa)

Figure 4d – NRF2 / TXNIP (61 kDa and 50 kDa)

Control Rnls- - - + + + - - - + + + TG

75Kd

37Kd

Scanned by C-DiGit Blot Scanner, LI-COR

Scanned by C-DiGit Blot Scanner, LI-COR

-15 Kd

-20 KdControl Rnls

- - - + + + - - - + + + TG

Anti-cleaved Caspase 3

Scanned by C-DiGit Blot Scanner, LI-COR

Control Rnls- - - + + + - - - + + + TG

Anti-NRF2 (61Kd)

Anti-Txnip (~50 Kd)

2

Figure 4d – phosphorylated eIF2alpha (38 kDa)

Figure 4d – phosphorylated IRE1alpha (110 kDa)

Figure 4d – phosphorylated PERK (170 kDa)

Figure 4d – total eIF2alpha (38 kDa)

Figure 4d – total IRE1alpha (130 kDa)

Control Rnls

- - - + + + - - - + + + TG

Scanned by C-DiGit Blot Scanner, LI-COR Anti-phospho-eIF2a

37Kd-

Scanned by C-DiGit Blot Scanner, LI-COR

Control Rnls- - - + + + - - - + + + TG

Scanned by C-DiGit Blot Scanner, LI-COR

37Kd-

50Kd- Control Rnls- - - + + + - - - + + + TG

Anti-Total eIF2a

Scanned by C-DiGit Blot Scanner, LI-COR

Control Rnls- - - + + + - - - + + + TG

Scanned by C-DiGit Blot Scanner, LI-CORAnti-Total IRE1a

3

Figure 4d – total PERK (150 kDa)

Figure 4e (Xbp1u:473bp; Xbp1s:447bp)

Control Rnls- - - + + + - - - + + + TG

150Kd-

250Kd-

Scanned by C-DiGit Blot Scanner, LI-COR

Anti-Total PERK 150 Kd

500 bp

400 bp

300 bp

200 bp

650 bp

non-speci!c Xbp1u 473 bp

Xbp1s 447 bp

Control Rnls- - - + + + - - - + + + TG

mut