overview of traditional chinese medicine - kaznmu · web viewoverview of traditional chinese...
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List of Accepted Abstracts
The First PartTraditional Chinese Medicine1. Overview of Traditional Chinese Medicine-----------------------------------Dong Jingcheng
（1）2. The Basic Structure of Traditional Chinese Medicine-----------------------Dong Jingcheng
（4）3. The Future of Traditional Chinese Medicine---------------------------------- Dong
Jingcheng（5） 4. The Literature Research of Ming Dynasty Physicians Treating Chest Pain
Syndrome------------------------------------------------------------- Li Fangshuai, An Dongqing（6）
5. The Preliminary Studies on Herbal Medicine Resources of Aconitum from Yili and Easten Region in Xinjiang------------------------------------------------------Liu ShuMin, Nie Jihong （7）
6. Zhisousan treating infections cough randomized clinical trials---systematic reviews----------------------------------------------Li Fengsen , Jing Jing, Li ChunYan, et al （8）
7. 120 Cases of clinical study on Side-lying position bowed their heads and shaking method in treating Cervical Spondylotic Radiculopathy----------------------------------------Liu JunChang, Muhetaer Ayoufu, Wang Xinjun, et al （8）
8. Body Constitution Classification (BCC) of Coronary Heart Disease (CHD) in the East, South, North of Xinjiang-------------------------------------------------------------Li Hui, An Dongqing（8）
9. Case-control Study of The Association between Northwest Dryness Syndrome and Chronic Gastritis-------------------------------------------------------------Zhang Huitian, Zhou Mingxin（9）
10. Effect of dipeptidyl peptidase IV in 3 extracts from plants of Xinjiang----------------------Yang ZhenZhen, Wang XueHua, Wang Ye, et al（10）
11. Effects of TianXiangDan on blood lipid and vascular endothelial growth factor in the arteriosclerosis in ApoE (-/-) gene knockout mice----------------- Jiao Yujie, An Dongqing（11）
12. Primary study on chemical composition and biological activity of Arctium tomentosum Mill----------------------------------------------------------------------Zhang Haoke, Tian Shuge（11）
13. Quantitate Chemical Composition and Chemical Pattern Recognition in Coriopsis Ticntoria from Different Areas----------------------------- Sun Zizeng, Zhang YanLi, Aytulun Simayi（12）
14. Regulation Effect of OboBiyanChongji on Expression of IL-2, IFN-γ, IL-13 and Nasal
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Mucosa in the Experimental Allergic Rhinitis in Rats-------------Jiang Liming, An Yang （12）
15. Research on Changes of Different Contents in Fried Aconitum pendulum Busch---------------- Wu Lin, Zhang Fan（13）
16. Research on Xinjiang Region Physical Type of Patients with Hyperuricemia on Behalf of Tea and Chinese Medicine Intervention--------------------------------------- Li Li, An Dongqing（13）
17. Safety Evaluation of Awei Capsule: Assessment of Acute and Subchronic Toxicity in rodent-------------------------------------------------Han Rong, Xue Jie, Nurmuhammat Amat（14）
18. Sophora alopecuroides extract on human coloncancer cell line HCT116 strains of chick embryo chorioallantoic membrane experimental studyon tumor angiogenesis--------Li Jingjie, Lv Shuqin（15）
19. Study on the difference between Uighur ethnic group and Han people who suffered from mild cognitive impairment (MCI) with electroacupuncture therapy-------Guo Hui, Liu Zhiyan（16）
20. Study on the Pharmacognosy, Chemistry and Acute Toxicity of Aconitum soongaricum Stapf---------------------------------------------------------------------Cai DongMei, Zhang Fan（16）
21. Study The Best Extraction With A ngeLica From Knee and ArthraLgia HerbaL Paste --------------------------------------------------------------------------S.Yun, G.Liang, W.XinJun（17）
22. TCM hung drag line drainage is the treatment of high anal fistula-----------Chang Zhongsheng, Yu Dandan （17）
23. The Effect of Sophora Alopecuroides Extract to the Receptor Tyrosine Kinases in Colorectal Cancer Cell Lines-----------------------------------------------------------Jiang Qin，Lv Shuqin（18）
24. The Clinical Observation of Tianxiangdan Capsule in Treating Angina Pectoris in Coronary Heart Disease (Syndrome of Qi Deficiency and Blood Stasis)-------------Song Xiaopeng，An Dongqing（18）
25. The experience of using dihuang pills to treat the chronic cough and asthma treatment of lung-------------------------------------------------------------------------------------Shi Suofang（19）
26. The influence of p38 MAPK pathway to asthmatic guinea pigs which Qufeng Xuanbi made-------------------------------------------------------------------------------------Shi Suofang（19）
27. The Studies of Regulating Blood Lipid and Antioxidant of Awei Capsules on Hyperlipemia Rats-----------------------------------------------------------------------------Cui Liang, Xue Jie （20）
28. The study of quality standards and determination of validity period of Awei capsules----------------------------------------------------------------------Hou MingQi, Xue Jie (21)
29. The Study on Medicinal Value and Reduce the Toxicity with Processed of Aconitum soongaricum Stapf in Xinjiang--------------------------------------------- Wu Chao, Jiang Lin（21）
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30. The study on sustained-release technology of Tianxiangdan capsules----Wang Jia, Jiang
Lin（22）31. Theory of Acupuncture "reinforcing" and "reducing"----------------Cai Hua, Yin
GaiZhen（23） 32. Assessment of using the Tianxiangdan Jiaonang Helpling Rehabilitation Therapy in Coronary
Artery Bypass Grafting---------------------------------------------Li Jingru, Wang Xiaofeng （24）
33. Clinical study of Differential Treatment of Traditional Chinese Medicine in Treatment of 210 Cases of Sub-health State in Urumqi city-------------------------------Liu Tao, Hu Xiaoling（24）
34. Coronary Heart Disease merge 2-hour postprandial glucose patient of Traditional Syndrome Differentiation and ZeXieDanMingYin intervention research---------------Zeng Lin, An Dongqing, Li Kaili, et al（25）
35. Experimental Study in Effects of Xu-zao-geng-ping Powder on the Histomorphology Change of Arteries and MMP-9, TIMP-1 in Climacteric Hypertension Rats---Li Hongxia, Lin Xue（26）
36. Experimental study on inflammatory factors in serum of rats with Rheumatoid Arthritis treated by PengCao Removing Bi-syndrome Decoction------- Tian Xinwei, Liu Chunjing（27）
37. Study on Influence of Xu Zao Geng Ping granule on Blood Pressure Variability of the Rats with Menopause Hypertension-------------------------------------------Gan Qinglei, Lin Xue（27）
38. The characteristic of the syndrome TCM distribution of femoral head necrosis in Urumqi region-----------------------------------------------------------------Jin Wuyong, Meng Qingcai（28）
39. The Correlation of Uighur and Han Nationality of TCM Syndrome Type of Premature Coronary Heart Disease with Blood Lipids and Inflammatory Factor----------------Zhai Xueqin, Wang Xiaofeng（29）
40. The Correlation of Uighur and Han Nationality of TCM Syndrome Type of Premature Coronary Heart Disease with Molecular Markers of Prethrombotic State----------------Liu Hao, Wang Xiaofeng（30）
41. The experimental study of Qihong Decoction on the effects in rat model with isoproterenol induced chronic heart failure-------------------------------------Wang Min, Wang Xiaofeng （31）
42. The Influence of Oubo Rhinitis Granule to Allergic Rhinitis Rats Th2 Cells and Its Factors、 IgE and Mast Cells---------------------------------------------------- Zhang Xingri, Li Yanhua（31）
43. The pathological effect of PengCao Removing Bi-syndrome decoction on synovial inflamation and bone erosion of rats with Collagen-Induced Arthritis------------Chen Chang, Liu Chunjing（32）
44. Treatment of QHD Tongluo Experimental Study of hyperuricemia-------Wang Danhui, Yushanjiang Aikemu（33）
45. The Influence of Xihong Kang Fang Using to the diabetes nephrosis patient phase III Hcy CysC and UAER----------------------------------- Feng Chengcheng, Yushangjiang
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Aikemu（33）46. Clinical Analysis of 200 Cases of Curative Effect Factors on Treating Knee Osteoarthritis
with Small Needle Knife-------------------------------------------------------Ma Lei, Meng QingCai（34）
47. The Study ofClinical Curative Effect Research of Jing Yi An Shen Powder on 104 Cases of Insomnia Treatment by Acupoint Application-------------------------------------Wei Qing, Hu Xiaoling(35)
48. Clinical Curative Effects of Repeated Shallow Puncture with Filiform-fire Needle on Herpes-zoster-----------------------------------------------AyijiamaliTuohuti, Liu Hongxia（35）
49. Clinical Effect of DLK-A for Treatment and Prevention of Metabolic Syndrome(MS), on Blood Sugar, Blood Pressure, Blood Lipids and Weight------------------Palidan Wushuer, Nurmuhammat Amat, Duolikun, et al(36)
50. Clinical observations on chronic prostatitis of dampenss-heat down-flowing stasis with qianlieshuang decoction--------------------------------------------------------Sha Mo, Ma Chao(37)
51. Clinical Study on Traditional Chinese Medicine combined with Acupuncture of Promoting the Recovery of Gastrointestinal Function in the Treatment of Postoperative Patients with Damp-heat Type ---------------------------------------------------Guli Shaera, Liu Zhiyan(38)
52. Decoction of Bushentongluo and Decoction of its Decomposed in Repair of Articular Cartilage Defects in Rabbits-------------------------------------------------Li Leijiang, Lu Yong(38)
53. Effect of Jangzhuoshengqingtang on Pathology organization morphological、 plaques area and Inflammatory Reaction in Atherosclerotic in Aorta of Apo-E-gene knockout Mice---------------------------------------------------------------------Wang Jipeng, Xin Xiaoyi(39)
54. Experimental Study on Effect of Aplastic Anemia Model Rat’s Regulation Factors IL–2、TNF–a Treated with Tonifying Kidney and Replenishing Essence Method---------------Xie Rong, Shan Lijuan (40)
55. Literature Research on Prescription Rule of Oral Drugs for Osteoarthritis-----------------Chu LiTao, Lu Yong(41)
56. Primary Immune Thrombocytopenia Different Syndrome Types and Platelet Parameters of Characteristics Research--------------------------------------------Wang Yahong, Bai Yusheng(42)
57. Qufeng antispasmodic to asthma scattered (wind phlegm wheezing) patients with pulmonary function, FeNO influence----------------------------------------------Bai WenMei, Li Fengsen(52)
58. Research on Catharsis and Moisturizes Theoretical Literature of Chinese Medicine Skin---------------------------------------------------------------------------Chen Lu, Zhou Guang(43)
59. Spleen detoxification soup murine spleen deficiency type psoriasis ET-1, E-selection and of PCNA influence-----------------------------------------------------Guo Shanshan, Liu Hongxia(44)
60. The Comparison on the Location of Insomnia According to Different Classification Traditional Chinese Medicine of Five-god Theory and Four Examinations---------Fan Xiufang, Zhang Xingping(45)
61. The comparison research of acupuncture and acupoint application in patients with AECOPD and their influence of lung function and quality of life -----------Shao Meimei, Liu Zhiyan(45)
62. The effect of jianpijiedu decoction on the vascular active factor of psoriasis model mice------------------------------------------------------------------------ Gao Yuan, Liu Hongxia(46)
63. The Experiment of Jian Pi jiedu decoction on Guinea Pig Model of Psoriasis with Spleen
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Deficiency Syndrome-----------------------------------------------------Guo Fei, Liu Hongxia(47) 64. The Experimental Study of Huangqi Guizhi Wuwu DecoctionT herapy on Failed Back
Surgery Syndrome-----------------------------------------------------Liu Kaiying, Huang Yifei(48) 65. The Impact of Yangyin Huowei Heji on Chronic Atrophic Gastritis Patients' Gastroscope
Histopathology and Hp-------------------------------------------Ding GuoNing, Zeng Binfang(48) 66. The Research of the Inhibition of Invasion and Metastasis of Colon Cancer Cells HCT116
after Administrate YiQiFuShengFang by ERK/MAPK Signaling Pathway-------------Han Jing, Lv ShuQin(49)
67. The Study on Inflammatory Factors Therapeutic Effect of DangGuiNianTongTang Pasty with MTX on Adjuvant Arthritis in Rats-----------------------------------------Yuan Li, An Liping(50)
68. The Clinical Research of Acupuncture Combined with Auricular Acupuncture for Patients with Acute Exacerbation of COPD to Improve their Quality of Life and Pulmonary Function------------------------------------------------------------------------Cao Lin, Liu Zhiyan(50)
69. Traditional Chinese Medicine theory and gynecological dietotherapy put medicine law research-----------------------------------------------------------------------Tan Rong, Liu Ailing(51)
70. Distribution characteristics of TCM syndrome and treatment on 2026 Cases of Sub-health Population in Urumqi City-----------------------------------------------Du Shuai, Hu Xiaoling(53)
71. Yang-yin-huo-wei Formula Treatment of Chronic Atrophic Gastritis Patients Report Outcomes and Quality of Life in Research -------------------------------------------- Dai Ming, Zeng Binfang(53)
72. Anti-hypertensive effects of the aqueous extracts of Chinese yam (Dioscorea opposita Thunb.） in Two-Kidney, One-Clip Renal Hypertension Rat model-------------------------- Nurmuhammat Amat, Palidan Wushuer, Sajida Abdureyim, et al(54)
73. Effect of TongFeiJingTanFang on Immunologic Function of Phlegm Heart Syndrom in Chronic Obstructive Pulmonary Disease-------------------------Bai Zhengping, Tan Guangbo, Hu Xuejun(55)
74. Effects of “Yang-Warming and Kidney Essence-Replenishing Herbal Paste” on Cold-related Asthma Exacerbation——A Report of 74 Cases-------- Tang Binqing, Shi Kehua, Li Xiaoqi, et al(56)
75. Huang Di Nei Jing health theory and the prevention and treatment for chronic obstructive pulmonary disease-----Xu YanLing56()
76. Influence of Compound Panax notoginsenosideGranules (CPNG) on bleomycin-inducedpulmonary fibrosis in rats-------------------Ling Chunhua, Wu Juanjuan(57)
77. Qufeng Xuanfei side clinical study of treatment of allergic cough ----------------------Xu Lihua, Li Lei, Huang Ye, et al(57)
78. Randomized Control Trial of JinhuaQinggan Granule in Treating Influenza with Wind-Heat Affecting Lung Symptom-Complex------------------------Li Guoqin, Liu Zhiguo, Liu Qingquan(58)
79. Scorpion - centipede on the rat model of asthma airway inflammation and airway remodeling in experimental research ------------------------Li Haiyan, Yang Peilan, Huang HaiYin, et al(58)
80. The application of Lung and Large intestine Interacts relations in COPD------------Geng Limei(59)
81. The effect of Er Chen Jie Han Particle for the treatment of OSAHS patients with Phlegm
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Damp Syndrome-----------------------------------------------------Wang Zhen, Yang Junchao, Shen Yingying, et al(59)
82. The search of basic law of the ancient physicians using the prescription medication to treat "Pulmonary heart disease in the remission stage "----------------Hu Xuejun, Bai Zhengping, Tan Guangbo et al(60)
83. Rharmacodynamic Research on Liver Protecting, Choleretic and Immune Regulating Function of Deduhonghua-7 Powder------------------BA Tu-de-li-gen，WU En-bao-yin，NA Ri-su, et al(61)
84. The Clinical Study Between Syndrome Type in TCM and the Serum Levels of IL-25 and Total IgE in Patients with Bronchial Asthma--------------------------------Liu ZongWei, Zhou XianMei(61)
85. Clinical Observation on Chronic Obstructive Pulmonary Disease of Acute Aggravating Stage Treated with Compound Fuercao mixture-------------------Shi KeHua, Zhe Zhe, Lv MiMi, et al(62)
86. Effective cases analysis of Professor Wu Yin-gen in differentiating and treating interstitial lung disease-----------------------------------------------------------------Shi KeHua, Xiong Bidan, Yu Xiao, et al(63)
87. Experimental Study of Type 2 Diabetes Animal Models through Decoction of Baihuginseng-chickpea Mixture------------------------------------------Ji Yan, Wang Cunfen(63)
88. Preliminary study on Preparation Technology、Quality Standard and Pharmacodynamic for Nigella Glandulifera Freyn Particle------------------------Xiao Qiong, Jiang Lin, Li Xiaojin(64)
89. Preliminary Study on the Chemical Composition of Nigella Glandulifera Preyn-------- Xu Jingjing, Xue Jie, Jia Xiaoguang, et al(65)
90. Kidney’s Concept of TCM and Mechanism, Method, Prescription, Drug & Application of Kidney-invigorating------------------------Wang GenFa, Dong JingCheng, Duan XiaoHong(66)
The Second PartUygur Medicine
91. Is “abnormal black bile” merely imaginary philosophical concept that has no medical meaning?-------------------------------------Halmurat Upur, HR Tang, Batur Mamtimin, et al(66)
92. Bioactive fraction of Abnormal Savda Munziq and its antiproliferative activity on HL-60 cells----------------------------------------- Murat Kizaibek, M.Baranov, Halmurat Upur, et al(68)
93. Effect of Xiatara on ultrastructure, protein and ergosterol biosynthesis in dermatophytes----------------------------------------------------Deng ShuWen, Halmurat Upur(69)
94. Establish the Alzheimer's disease carrying abnormal savda syndrome rat model and observe the effects of formulae on it-----------------------Palidan Wushuer, Nurmuhammat Amat, M.Baranov, et al(71)
95. Establishment of hepatocarcinoma carrying abnormal savda animal model and it`s morphological----------------------------------------------------Yusup Tuerxun, Halmurat Upur(72)
96. Experimental Study on Caries Prevention by Aleppo Gall----------Z.Jin, Halmurat Upur, Baranov M, et al(73)
97. General toxicology studies of Xi pa yi Kui Jie’an enema------------------------ Kasimjan
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Aximjan, Halmurat Upur(73)98. Abnormal Savda Munziq and Munshil’s effect on the gene regulation, oxidative stress
induced cell damage and apoptosis-------------------- L.Lin, L.Gogol, Halmurat Upur, et al(74)99. Mechanism research of Gujingmaisiha treatment for premature ejaculation (PE)----Song
GuoHong, S.Nelski, Halmurat Upur(75)100. Metabonomic studies of abnormal Savda syndrome complexity diseases in Traditional
Uighur Medicine------------------------------------------- Xu WeiFang, N.Tatiana, T.Aji, et al(76)101. Studies on platelet parameters and hepatic function changes of COPD patients with syndrome
of kidney deficiency and stagnation of phlegm---------------------- You Na, Halmurat Upu(77)r
102. Studies on the Anticancer and Reversing Multidrug resistance effects of Abnormal Savda Munziq Total Flavonoids--------------------------- Maynur Eli, M.Baranov, Halmurat Upur(78)
103. Study on anti-tumor mechanism of Abnormal Savda Munziq in vivo based on transplanted S-180 and EAC tumor model----------------------------------Anwar Aikemu, Halmurat Upur(79)
104. Study on the Possible Mechanisms of Xinjiang Red Raspberry Fruit Extract in Reducing Blood Pressure and Alleviating Oxidative Stress in SHR Rats--------------------------- H.Jia(83)
105. The Abnormal Savda Syndrome CD41, CD62p and fibrinogen clinical analysis of change---------------------------------------------------------------Cao XiaoBin, Halmurat Upur(85)
106. The clinical report of Awrixim syrup to treat the Vasculitis patients, one case------ G.Abduriyim, P.Ablimit, R.Jappar, et al(85)
107. The Study of Basic Exploration and Application of Uighur Medicine Xipayi Mouth Rinse in Treatment of Gingivitis --------------------------------Habiba, S.Nelski, Halmurat Upur et al(86)
108. Study on the anti-inflammatory and anti-asthmatic mechanism of Hyssopus officinalis.L active fractions---------------------------------------------- A.Nurahmat, N.Rozi, A.Rozi, et al(87)
109. A study of the effects of the leaf extract of Cydonia Oblonga Miller on blood hemostasis, coagulation and fibrinolysis in mice.------------------------Anwar Umar, Zhou WenTing, Ibadet Tohti, et al(88)
110. About the exploration of Bloodletting therapy of Uighur Medicine------------Amier Alye, Tuersunjian Tohsun(89)
111. Analysis of intestinal bacterial communities in four group abnormal hilit hyperpietics and normal hilit of Uighur nationality---------------------------Jiang Wei, Halmurat Upur, Delixiati Yimiti, et al(89)
112. Ancient Uighur drug treatment of coronary heart disease medication compatibility law----------------------------------- Mamatyili Rumat, Aniwar Talip, Abduwar Abulak, et al(90)
113. Anti-carcinogenic effects of the polyphenol of Abnormal Savda Munziq in association with its cytotoxicity , apoptosis-inducing properties and telomerase activity in human cervical cancer cells (SiHa)------------------------------- G.Abliz, Ahmatjan.Mamat, W.XinLing, et al(90)
114. Cognitive enhancement and neuroprotective effects of Abnormal Savda Munziq, a formulation of Traditional Uighur Medicine, in a rat model of Alzheimer’s disease -----------------------------------------Palidan Wushuer, Nurmuhammat Amat, Halmurat Upur(91)
115. Composition of the essential oil and antioxidant activity of several extracts of Moluccella laevis L. (Lamiaceae) --------------------------------Güvenç, A, Özek, G, Hürkul, M.M, et al(92)
116. Composition of the essential oil and antioxidant activity of several extracts of Moluccella
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spinosa L. (Lamiaceae) -------------------------------Güvenç, A, Özek, G, Hürkul, M.M, et al(92)117. Determination peptides compositions of Chickpea Sprouts by high performances capillary
electrophoresis------------------------------------------A.·Yili , Q. L. Ma ， Zhang Junping, et al(93)
118. Effect of Cydonia Oblonga on experimental Thrombosis in rats------------Zhou WenTing, Anwar Umar, Wuliya Yimin, et al(94)
119. Effects of Ocimum Basilicum L. extracts on Lipid Metabolism and Oxidant Status in Hyperlipidemic rats---------------------------------------------Anwar Umar, Zhou WenTing, Wuliya Yimin, et al(94)
120. Effects of soybean peptide on neuroendocrine immune network, memory, hematology and plasma biochemistry in young healthy volunteers----------------Nurmuhammat Amat, Palidan Wushuer, Guzalnur Imam, et al(95)
121. Experimental study on Vasodilation of Traditional Uighur Herb for Vitro Vascular--------Lan Wei, Jing YuXia, Halmurat Upur(96)
122. Retrospective analysis of hypertension and hypertensive intracerebral hemorrhage in hospital-ized patients of Uighur and Han nationnality in Hami district of Xinjiang----------------Aniwar, Abulymit, Wang Kun, et al(96)
123. Hepatoprotective effects of Cichorium Glandulosum leaf extract against d-galactosamine-induced Liver injury in mice----------------Imam G, Yusupjan, Aynur, et a(97)l
124. Influence of the Uighur Medicine sand therapy on hemorheology of the rabbit experimental knee osteoarthritis and FEM analysis---------Mahemuti D, Halmurat Upur, Kong CY, et al(97)
125. Inhibitory effect of Pomegranate Blossom Flower (PGf) extract on Influenza virus neuraminidase activity and induced Cytotoxicity--------------------------------------Lan Wei，Lan WenJun，Halmurat Upur(98)
126. Preparation and in vitro anti-cancer activities of flavonoids from Xinjiang Glycyrrhiza Inflata ------------------------------------------------ Mourboul Ablise, Sheng Lei, Rena Kasim, et al(98)
127. Report of the eels and other drug treatment of facial paralysis embolism---------------- Mamat Hujy, Gao Jianping(99)
128. Screening of quantitative diagnosis indices of Typing Abnormal Hilit syndrome in Uighur Medicine using delphi method to survey: 180 cases of malignant tumor------------Ayinuer Mamatisidike, Halmurat Upur，Erxiding, et al(100)
129. The curative effects of 51 cases with Uighur Medicine therapy on Urticaria------- Mahmutjian Ahmat, Mamatjian Zayit(100)
130. The systematic reviews of the Uighur Medical abnormal humoral typing to the treating knee osteoarthritis--------------------------------------------- J.XiaoYue, M.kelimu, G.ShiMin, et al(101)
131. Treatment of 225 cases of hair loss according to Uighur Medical temperament theory analysis alopocia-----------------------------------------------------------Sidik Ramat, Nuermanguli Aly(101)
132. Tradition, current situation and perspective of traditional Uighur Medicine in China---------------------------------Nurmuhammat Amat, Palidan Wushuer, Aziz R A, et al(102)
133. Treating report about 190 patients with kidney stone patients treated by Uighur Medicine---------------------------------------------------------------------Abduwayit Nurahmet(102)
134. A clinical report of 31 cases using Uighur Medical treatment of herpes zoster ------------- Nuermangul Alya , Sidik Ramat, Tajigul Mama(103)t
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135. Uighur Medicine fusion of East-West cultural factors-------------------------Wang XingYi(103) 136. The effect of Abnormal Savda Munziq on Hippocampus BDNF, 5-HT1A mRNA expression
in depression animal models and Abnormal Savda Syndrome animal models-------------------Upur Hanzohra, Abduwayit Nurahmet, T.Kamil, et al(103)
137. The construction of the Uighur pharmaceutical scientific research information support platform system based on Cloud Technology------------------- ZhouYi, Dilixiati Yimiti, Luo XueQiong, et al(104)
138. Chemical Constituents in Essential Oil from Hyssopus officinalis.L by Steam Distillation and SFE-CO2 by GC-MS------------------------Mahsum Ekim，Nurjan, Abdiryim Yusup, et al(104)
139. Preliminary study on the anti-inflammatory effect of the extracts of Hyssopus officinalis.L----------------------------------Mahsum Ekim，Nurjan, Abdiryim Yusup, et al(105)
140. Abnormal Savda Munziq Improves the Biological Syndromes of CUMs induced Depression Animal Models------------------------Abdiryim Yusup, Nurahmat A, Guzalnur Imam, et al(105)
141. Abnormal Savda Munziq, an Herbal Preparation of Traditional Uighur Medicine, Regulates the Neuroendocrine Function of CUMs induced Depression animal models------------Abdiryim Yusup, Hanzohra Upur, Guzalnur Imam, et al(106)
142. Clinical Research on Treatment of Knee Osteoarthritis by the instrument of Heat Conduction Sand instrument in Uighur Medicine----------------------------He ChunHui, Halmurat Upur(106)
143. Effect of pomegranate ethyl acetate extract on the function of rat islet cells --------- Yao Hai, Yang WeiXing, Xu YongHua, et al(107)
144. Establishment of hepatocarcinoma carrying abnormal savda animal model and it`s morphological study-------------------------------------------Yusup Tuerxun, Halmurat Upur(107)
145. Metabonomic Studies on Abnormal Savda Patients with Type 2 Diabetes Mellitus Using NMR Spectroscopy------------------------------- Mao XinMin, Lang Jun, Ma XiaoLi, et al(108)
146. Studies of chemical constituents and antioxidant activity of Jujubae Fructus in Xinjiang ---------------------------------------------------------------------Wang DongDong, Tian Shuge(109)
147. Studies on Chemical Constituents and anti-tumor of essential oil from the seeds of Ferula sinkiangensis -----------------------------------------------Wang Yue1, Jiang Lin1, Li Xiaojin(109)
148. Study on Quality Standard and Fingerprint of Ziziphora clinopodioides Lam-------Yu Qian, Tian Shuge(110)
149. Study on the Uighur Medicine Compound of the Anticancer Activity Parts of Abnormal Black Munziq—Based on the “TCM Components” Mode------------Yang Juan, Zhang Fan, Halmurat Upur(111)
150. The impact of Hypoglycemic Ziyabiti Tablet (HZT) extracts on type 2 diabetes mellitus rats and its activity in cultured liver cells---------------------- Li Guirong, Fan Yong, Wang XiaoFeng, et al(112)
151. The study on the development of the Xipayisolid-gingival buccal tablets---------Ma Pingping, Nie JiHong(113)
152. Toxicological evaluation aqueous extract of the galles of Quercus infectoria olivin rodent-----------------------------------------Mubarak, Nurmuhammat Amat, Kasimjan, et al(113)
153. Dialectical Analysis of exercise-induced fatigue patients according the Uighur Medicine humoral quality ----------------------------Ailyjan Asla, Meng Qingcai, Liu Zhenfeng, et al(114)
154. Comparative study Uighur Medicine sand therapy with other regional sand therapy， sand characteristics----------------------Abudusalam Saidula, Osman Yasin, Satar Kurban, et al(115)
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155. The Relationship between toxic electron microscopy examination and alkaloid amounts in Datura stramonium L seeds of Xinjiang------------ R.slam, Y.Mamat, A.Abdugheni, et al(115)
156. Effect of psychological factors on vitiligo patients’hypothalamus-pituitary-adrenal axis and intervention study----------------------------------------------- Aikebaier Anzhaer 1, Silafu Aibai 1*, Tuerxun Wufuer, et al(115)
157. Arthroscopic Debridement Combined with Uighur Maizhuni Treatment of Knee Osteoarthritis andomized Controlled Trials--------------------------Yuan Guoxiang, Lu Yong(116)
158. Association analysis Uighur Medicine's combined modality therapy with ordinary psoriasis patien's cytokine and immunologic mechanism-------------- Riziwan Wumaier 1, Aikebaier Anzaer 1, Silafu Aibai, et al(117)
159. Study On Xanthine Oxidase Inhibitory Actvity In Capparis Spinosa.L By Spectrophotometry---------------------------------- A.Rahman,A.Tax, Nurmuhammat Amat(117)
160. Clinical effecacy observation Uighur Medicine Preparetion Denar on the patients with viral hepatitis with Cholestatic syndrome------------------------------------Dmytriy Sebrebryakov(118)
161. Clinical Observation of Wujing Ointment in Reducing Adverse Reactions attached to Asthenia of Spleen and Pathogenic damp of FOLFOX4 in Treating Cancer of Colorectum ------------------------------------------------------------------Xiao ChunXia,Zhang HongLiang(119)
162. Clinical research of auxiliary therapy effect of Turnips on tuberculosis patients------ A.Pattar, Nurmuhammat Amat，Halmurat Upur , et al(119)
163. Clinical Therapeutic Efficacy and Security Assessment of Uighur Medical treatment for Vitiligo with Uighur Medicine-------------------------------------- Tursun Wuphour, Yüsup Metenur, Silafu Aibai, et al(120)
164. Correlation Analysis of Syndrom Differentiation of Vitiligo and Its Munzich and Mushil Therapy Based on Traditional Uighur Medicine Theory------- Silafu Aibai, Tuerxun Wufuer, Aizezi Yasen, et al(121)
165. Detection of neuroendocrine and immune factors before and after traditional Uighur Medicine treatment of vitiligo Uighur and it’s significance---------- Aikebaier Anzhaer, Silafu Aibai, Tuerxun Wufuer, et al(122)
166. Discussion on the relationship between vitiligo and peripheral blood in traditional Uighur Medicine------------------------------------------------------- R.Umar, J.Abduwayet, T.Tursun(122)
167. Effect of Euphorbia humifusa active component on the cell membrane biosynthesis and gene expression of metalloprotease(MEP), subtilisin(SUB) of Trichophyton rubrum------ Li ZhiJian, Zhao MingYue, An HuiXia, et a(123)
168. Effect of Uighur Medicine Maizhuni on the Expression of MMP-1, MMP-3, MMP-13, TIMP-1, IL-1β, and Aggrecan in Rat Osteoarthritis Models-------------Zhang Yaowu, Meng QingCai(123)
169. Research of Quality Control about Abnormal Savda Munziq Granular andα-Glucosidase Inhibitors--------------------------------------- Xin LuDe, Tian ShuGe, Halmurat Upur, et al(124)
170. Retrospective Survey and Analysis on Ulcerative colitis Treated with Uighur Medicine-------------------------------------------- Turgun Amar, Jurat Tohti, Sirap Abai, et al(125)
171. Retrospective Survey, Research and Analysis on Osteoarthritis of the Knee treated with Uighur Mdicine--------------------------------------------------------- Jurat Tohti, Turgun Amar,
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Sirap Abai, et al(125)172. Studies on Anti-Aphthous Ulcer and Mechanisms of Galla Turcica Extract treating Recurrent
Aphthous Ulcer in experimental animals------------------ Fan XiaoHong, Li ZhiJian, Silafu Aibai, et al(126)
173. Study of in vivo - in vitro Anti- Influenza virus and Anti-inflammation and immune function of Artemisia rupestris extract-------------------------------- Meng FanLong, Li ZhiJian 1,2, Silafu Aibai, et al(127)
174. The Nymphaea alba with oxidative damage protection to human umbilical vein endothelial cells---------------------------------------------------------------------Lan Wei, Halmurat Upur(128)
175. Uighur Medicine Capparis spinosa treatment of 67 Cases of chronic arthritis ------------Obul Hupurjan(128)
176.Uighur Medicine Karapxa decoction as a hypouricemic agent: reduces serum uric acid level, inhibits liver xanthine oxidase in hyperuricemic mice model and, scavenge free radicals in vitro------------------------Nurmuhammat Amat, Palidan Wushuer, Halmurat Upur, et al(129)
177. Kali Zira （Vernonia anthelmintica Willd ）injection induced allergic reaction in 6 cases---- R.Umar, J.Abduwayet, Tursun Wuphour, et al(129)
178. Uighur Medicine Xipayi Kui Jie’an effective Gene Expression Profiles on Model of ulcerative colitis rat colon organization------------Yunusi Ki，Z.Jingping，Y.Mijit，et al(130)
179. Uighur Medicine Xipayi Kui Jie’effect appreciation and apoptosis on HT-29 human colon cancer cells--------------------------------------------- K.Yunusi，Y.Mijit，Z.JingPing，et al(130)
180. Concept, Classification and Analysis of Uighur Medical toxic drugs in Traditional Uighur Medicine Hospital of Xinjiang Uighur Autonomous Region -------------------------A.Turdi(131)
181. Clinical effect observation on treatment Adenomyosis with Traditional Uighur Medicine------------------------------------------------------------------------------------ M.Abliz(132)
The Third PartMongolian Medicine
182. Clinical report of the Mongolian psychosomatic medicine interaction therapy for the treatment of complex disease --------------Na Gong Bilige， A Runa， Chang Ming， et al(133)
183. On the Mongolian understanding and dialectical treatment of pigmented purpuric dermatoses--------------------------------------------------------------------------Chen ShaNa(133)
184. Persistance in the Combination of Scientific Culture and Humanities Culture in the Modernization of Mongolian Medicine--------------------------------Te Buqin, Bai Changxi(134)
185. The antibacterial and diuresis action of Mongolian medicine “yunga-4”---------------------------------------------------------------------------------------------------Guqid(134)
186. The sand treatment, Mongolian medicated bath, Mongolian medicine for oral administration: three combined treatments of rheumatoid arthritis-------------------------A la Teng Chu Lu(134)
187. The experience of cervical cancer treatment combined with Mongolian medicine European medicine-------------------------------------------------------------------------YH Bai, CM Wang(134)
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The fourth PartIntegrative Medicine
188. A Clinical Study on Changes of TCM Syndrome and Relevant Factors in Patients with Esophageal Carcinoma before and after Radiotherapy------------------Wu Langjie, Du YeQin(136)
189. Analysis of Bacteria Flora in Nasal Cavity on 102 Cases Acute Asthama-----------Ma Huan, Li FengSen(136)
190. BiPAP combined with Acupuncture in the treatment of Chronic Obstructive Pulmonary Disease complicated with Pulmonary Encephalopathy research---------- Wei Chenggong, Fu Ziyi, Liu Xiaohong, et al(137)
191. Clinical Evaluation and Lung Function of Plastering Therapy of TCM for Chronic Obstructive Pulmonary Disease in Stable Phase in China: A Systematic Review of RCTs------------------------------------------------------Li FengSen, Gao Zhen, Jing Jing, et al(138)
192. Comparison between the Mongolian and Western Medical Understandings of the Regularity of Infectious Disease Course Development ------------------Tu Men Wu Li Ji, Li Guo Hua(138)
193. Correlation between prothrombotic state markers and TCM syndrome type in Uighur and Han patients with premature coronary heart disease ---------------- L.Hao, Wang XiaoFeng, Zhai Xueqin, et al(139)
194. Different Syndromes in Patients with Asthma-related Changes in Immune Indexes--------Su Jun, Li FengSen, Ma Huan, et al(139)
195. Explore the scientific meaning of “Lung Governing Skin and Hair” from SIgA in secretions of asthma, urticaria patients-----------------------------Zhang YanLi, Li FengSen(140)
196. Intestinal Microflora structure and function analysis in Traditional Uighur Medicine syndrome of asthma------------------------------------------------Li ChunYan, Halmurat Upur(141)
197. Study on Metabonomics Common Characteristics of COPD about Traditional Chinese Medicine Kidney Depletion Phlegm and Blood Stasis Syndrome and Uighur Medicine Abnormal Savda Syndrome-----------------------Xu WeiFang, Halmurat Upur, Li FengSen(141)
198. Observing the clinical efficacy of combined treatment of Traditional Chinese Medicine and Western medicine to ventilator-associated pneumonia---------Zhang YanPeng, Lv ShuQin(143)
199. Plasma metabonomics study on the asthma diagnosed as Shenxu-tanyu syndrome in Traditional Chinese Medicine with NMR---------------------Yan XingHai, Halmurat Upur(144)
200. Serum Metabonomics Research on Shenxu-tanyu Syndrome Type 2 Diabetes Mellitus Based on NMR--------------------------------------------------------------------You Li, Halmurat Upur(145)
201. Studies on Traditional Chinese Medicines----------------------------Brantner Adelheid, Lower-Nedza Agnieszka, Shi YuJing(145)
202. Study of acupoint application therapy on rat prothrombotic state and establishment of the corresponding animal model ----------------------------------Zhang Wenhui, Song XiaoPing(146)
203. Study on Cytokine Regulation Mechanism of Downregulating Eosinophils By Tonifying Shen in Traditional Chinese Medicine to Prevent Asthma Relapse-----------------Zhang WenBin, Mao Bing(147)
204. Study on Esophageal Carcinoma Traditional Uighur Medicine Syndromes and the Change of T-lymphocyte Subsets and NK cells of Patients Before and After Radiotherapy----------Zhou
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Jie, Du YeQin(148) 205. Study on immune and endocrine disturbance of bronchial asthma by Traditional Chinese
Medicine, Uighur Medicine and Western Medicine------------Li FengSen, Halmurat Upur(149)206. Study on Neuro-Endocrine-Immune disturbance of Type-2 diabetes Millitus by Traditional
Uighur Medicine and Western Medicine--------------Mutallip Obulkasim, Halmurat Upur(151)207. Study on the Phlegm-Stasis Syndrome in Complex diseases and the Prethrombotic
State------------------------------------------------------------------ S.Abdurhim,Halmurat Upur(153)208. Study on relationship between TCM syndrome differentiation and Neuroendocrine-
Immune-Netwok in Type-2 diabetes----------------------Palidan Wushuer, Halmurat Upur(154)209. Study on the relationship between Qi asthenia phlegm-stasis syndrome of the malignant
tumor and Immunity Cell and Hormone secretion ---------------------------------Wang Qiaolin, Du Yeqin, Halmurat Upur, et al(154)
210. The clinical observation of the fixation in the treatment of acute ankle sprain in Traditional Uighur Medicine--------------------------------------------------------------Jin Hao, Lv FaMing(155)
211. The Clinical Research of Acupuncture Combined with Auricular Acupuncture for Patients with Acute Exacerbation of COPD to Improve their Quality of Life and Pulmonary Function-----------------------------------------------------------------------Cao Lin, Liu Zhiyan(156)
212. The correlation of TCM syndrome with the BODE index and quality of life for patients with COPD at stable phase-------------------------------------------------------Zhou Wei, Mao Bing(156)
213. The effect of Tanreqing injection on lipopolysaccharide-induced airway inflammation, mucus hypersecretion in rats and their own dose-response relationship--------------Zhou YunQing, Mao Bing(158)
214. The Modern and Traditional Uighur Medicine research of neuro-endocrine-immune in malignancies------------------------------------------------------------Zhang Li, Halmurat Upur(159)
215. The Observation of Curative on Extracorporeal High-Frequency Hyperthermia Combined with Yangzhengxiaoji Decoction ’s Treatment of Advanced Non-small-cell Lung Cancer----------------------------------------------------------Wu ShanShan, Zhang HongLiang(161)
216. The relationship between Bronchial asthma TCM syndrome differentiation and their NE 、 DA 、 5-HT changes--------------------------------------------------You LiNa, Halmurat Upur(161)
217. The relationship between tumor patients’ plasma monoamine neurotransmitter changes and their Traditional Uighur Medicine syndromes------------------------------------Chen Qingying, Du Yeqin, Halmurat Upur, et al(162)
218. The study between IFN-γ methylation and Chinese medicine syndrome type in subsets of allergic rhinitis patients between Uighur and Han in Ili of Xinjiang-----------------------Zhang QingQuan, Gao Li(163)
219. The study of the improvement of clinical effect about “Reinforcing Qi and Activating Blood” in Stable Chronic Obstructive Pulmonary Disease (Qi Deficiency and Blood Stasis Syndrome)-----------------------------------------------------------------------Min Jie, Mao Bing(164)
220. The Study on Levels of Immunoglobulin and Complement in Henoch-schonlein Purpura Patient with Different Syndrome----------------------------------Zhang Cheng, Bai Yusheng(165)
221. The study on the imbalance of Regulatory T cells/Th17 cells in the different Traditional Chinese Medicine syndromes of asthma-------------------------------Yan Lu, Zhou Xianmei(166)
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222. To study allergic rhinitis of TCM and IL-4 gene methylation between Uighur nationality and Han people comparative in Yili of Xinjiang---------------------------------Liu FuTai, Gao Li(167)
223. To study the relative of Kidney asthenia blood stasis type deafness and mtDNA 12s rRNA gene mutation in deafness of Uighur ethnic in Xinjiang region of China------------------------Su Xuefen, Li Yanhua(167)
224. Oncothermia combination with Traditional Chinese Medicine: network approach------------------------------------Hegyi Szasz A, G. Andocs, et al(168)
225. Airway inflammation and hypothalamic-pituitary-adrenal axis function in kidney-yang deficiency type of Chinese medicine in asthma and chronic obstructive pulmonary disease--------------------------------------------------------- Du Yijie, Li Bei, Cao Yuxue, et al(169)
226. Baicalin restores corticosteroid sensitivity in cigarette smoke induced inflammation by modulating HDAC2 in vivo and in vitro----------- Li Lulu, Wu JinFeng, Liu BaoJun, et al(170)
227. Establishment and comparison of combining disease and syndrome model of asthma with ‘kidney Yang deficiency’ or ‘abnormal Savda’---------------- Li Bei, Luo QingLi, Mammat Nurahmat, et al(170)
228. Icariin attenuates LPS-induced acute inflammatory responses: Involvement of PI3K/Akt and NF-κB signaling pathway------------------- Xu Changqing, Liu BaoJun, Wu JinFeng, et al(173)
229. Icariin inhibits corticosterone-induced apoptosis in hypothalamic neurons via the PI3-K/Akt signaling pathway--------------------------Zhang Hongying, Liu BaoJun, Wu JinFeng, et al(173)
230. Pharmacokinetics of forsythoside after intravenous administration in beagle dogs--------Shi Rong, Xuan ZhenYu, Ma YueMing, et al(174)
231. Research on the correlation between expression of foxp3 protein and airway inflammation and the effects of Icariin in a mouse model of asthma----------------- Jin Hualiang, Li Bei, Luo QingLi(174)
232. Some Chinese Medicine Components Attenuating Oxidative Stress and Airway Remodeling in COPD Rat via Keap1-Nrf2-ARE Signaling Pathway-------------------- Lv Yubao, Li Lulu, Sun Jing, et al(175)
233. The Effects of Icariin, Baicalin on Keap1-Nrf2-ARE Signaling Pathway and Beas-2B Cells Induced by Cigarette Smoke Extract (CSE)------------- Lv Yubao, Li Lulu, Sun Jing, et al(176)
234. The Effects of some Chinese Medicine Components on Oxidative Stress and Airway Remodeling of COPD Rats-------------------------------- Lv Yubao, Li Lulu, Sun Jing, et al(177)
235. The research on the relationship between compatibility of Buzhongyiqi Decoction and immune regulation------------------------------Luo Qingli, Dong JingCheng, Guo Xin, et al(180)
The Fifth Part Modern Medicine236. A comparison of the serum and sputum inflammatory mediator profiles in asthma and COPD
-------------------------------------------------Cao Yuxue, Gong Weiyi, Zhang Hongying, et al(181)237. A new type health food supplement with analgesic and Anti-inflammatory Activities, ard-
p401, but without adverse reactions ------------Michio Katagi， Akio Yasukawa， Chiaki Kudoh，et al(181)
238. A study of 406 cases with fever in the year between March 2009 and March 2010-----Li
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Zheng, Zhang Yanxin, Li FengSen(182)239. Anti-inflammatory and endocrine-regulated effects of icariin on cigarette smoking induced
chronic obstructive pulmonary disease in rats --------------Zeng LiXuan, Gong Weiyi, Wu JinFeng, et al(183)
240. Anti-inflammatory effects of icariin on cigarette smoke induced inflammatory responses in mice-----------------------------------------------------------Li Lulu, Wu JinFeng, Liu BaoJun, (183)
241. Application of the Delphi method of development of chronic obstructive pulmonary disease "fast"、"slow" criterion--------------------------------Li FengSen, Gao Zhen, Jing Jing, et al(184)
242. Baicalin is anti-inflammatory in cigarette smoke-induced inflammatory models in vivo and in vitro: A possible role for HDAC2 activity----------Li Lulu, Wu JinFeng, Liu BaoJun et al(184)
243. Calcineurin/NFAT signaling pathway mediates ET-1-induced proliferation by regulating phosphodiesterase-5 (PDE5) in pulmonary artery smooth muscle cells------------Lu Jiamei, Xie Xinming, Han Dong, et al(185)
244. Catgut implantation at acupoint on the effects of infertility of PCOS rats -------------------------------------------------------------------------------Tian Yali, Yin GaiZhen(185)
245. Clinical value of serum procalcitonin and C-reactive protein in diagnosis of bacterial infections ----------------------------------------------------------Wei Yanrong, Yang Huiqing(186)
246. Comparison of standard sample preparation methods with real time fluorescence quantitative PCR of Bacteroides thetaiotaomicron in intestinal from db / db type 2 diabetic mellitus mice-------------------------------------------------------Yi JinYang, Wang Ye, Zhu Ming, et al(187)
247. COPD xide measurement and influencing factors------------------- Wang Nana, Li FengSen, Bai WenMei, et al(187)
248. Correlation analysis on acute exacerbating COPD patients FENO and pulmonary function-----------------------------------------------Zhai Yujiang, Li FengSen, Xu Qian, et al(188)
249. CPPE (Compound of Pomegranate Peel Extraction) Reduce Adipogenesis Through Inhibit Mitotic Clonal Expansion-----------------Wang LiFeng, Tao YiCun, Zhang MinFang, et al(188)
250. Different Type Certificate in Patients with Asthma who Body Fluids, and Peripheral Blood of the Change of the Relevant Immune Index--------------------------------Su Jun, Li FengSen(189)
251. Discrimination of Type 2 diabetic patients from healthy controls by GC metabonomics method based on their serum fatty acid profiles-------Ma XiaoLi, Tian Lan, Li linlin, et al(190)
252. Distribution and Prevalence of Human Papillomavirus (HPV) in Uighur Women in China: A Population-Based Genotype Study-----------------------------------------------L.Hua, G.Abdixkur, Nurmuhammat Amat, et al(190)
253. Effects of baicalin on airway remodeling in asthmatic mice----------------Sun Jing, Li Lulu, Wu JinFeng, et al(191)
254. Expression of aquaporin 1 increases proliferation and metastasis potential of lung cancer----------------------------------------------------------Wei Xiaobai, Dong JingCheng(191)
255. Icariin attenuates airway inflammation and eosinophil infiltration by suppressing eotaxin and CCR3+ production in acute and chronic mouse models of allergic asthma --------------- Cai Cui, Du WenJing, Li Lulu, et el(192)
256. Icariin attenuates glucocorticoid insensitivity mediated by repeated psychosocial stress on an ovalbumin-induced murine model of asthma ---------------------------------------------Li Bei, Duan Xiaohong, Wu JinFeng, et al(193)
257. Icariside II Induces Apoptosis of Melanoma Cells in vivo through the Downregulation of
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Survival Pathways--------------------------Wu JinFeng, Xu JinHua, Erika A. Eksioglu, et al(193)258. Idiopathic acute eosinophilic pneumonia, one case of clinical features of analysis------Zhou
LinFu, Zong XiaoFu (194)259. Impact of psychosocial stress on airway inflammation and its mechanism in a murine model
of allergic asthma-----------------------------------Li Bei, Duan Xiaohong, Wu Jinfeng, et al(194)260. Intestinal bacteria support HIV infection？ ------------------------Shu ZhanJun, Li FengSen,
D.Tuerhong, et al(195)261. Metabonomic classification and detection of small molecule biomarkers of malignant pleural
effusions----------------------------------------------Zhou XianMei, He CuiCui, YuMei L et al(196)
262. Mitochondrial gene mutations in Uighur patients with type 2 diabetes mellitus in Xinjiang-------------------------- S.Abuduryim, Nurmuhammat Amat, Halmurat Upur, et al(196)
263. More attention should be paid to mast cells/basophils than to IgE in allergy: proposed procedure for diagnosing allergic disorders---------------------------------------He Shaoheng(197)
264. Moving and Breathing training on short-term effect of the stable COPD patients-------------Li FengSen, Li ChunYan, Du Lijuan, et al(198)
265. Neuroprotective effects of icariin on corticosterone-induced apoptosis in primary cultured rat hippocampal neurons-------------------Liu BaoJun, Zhang Hongying, Xu Changqing, et al(198)
266. Pathogenesis of idiopathic pulmonary fibrosis:from initial apoptosis of epithelial cells to lung remodeling?------------------------------------------------------Jin Hualiang, Dong JingCheng(199)
267. Preliminary Analysis of the miRNA Expression between Fresh and Old Peripheral Anticoagulant Blood-------------------------------------- Zhu ManLi, Jiao Yi, Wang Ye, et al(199)
268. .Preliminary Analysis of Transcription Level of FOXOs Genes in Patients’ Feet and Ankles Who Suffer Xinjiang Uighur Congenital Clubfoot----------------Dong HaoJie, Lv FaMing(199)
269. Prethrombotic State is a common manifestation of chronic disease: A clinical study ------------------------------------------- T.Aji ， S.Abudoureyimu, Nurmuhammat Amat, et al(200)
270. Puerarin effect and mechanism of inhibition of airway inflammation and airway remodeling in chronic asthma model-----------------------Zhou LinFu, Wang Wenlu, Li Hongyan, et al(201)
271. Regulation of STAT signaling in mouse bone marrow derived dendritic cells by respiratory syncytial virus-------------------------------Jie Zhijun, Darrell Dinwiddie, Albert Senft, et al(202)
272. Research on expression regulation of relevant cytokines of Th17 in COPD Mouse lung tissue--------------------------------------------------------Wang Jing ， Li FengSen，B.Aishan(202)
273. Seeking best menu of walking exercise for a healthy volunteers through the regulation of peripheral white blood cells in number----------------Nobuo Yamaguchi, Yimit D, Nurmuhammat Amat, et al(203)
274. Significance And Enlightment Of The Discovery On The Blood, Circulation Driven By respiration And Of The Study On The Mechanism Of The Day-Sweat And The Night-Sweat-----------------------------------------------------------------------------Li Zhigang(203)
275. The apigenin raised CD4 + CD25 + Treg cells and inhibit the study of the role of airway inflammation in acute asthma model------------------------Zhou LinFu, Li Hongyan, Wang Wenlu, et al(204)
276. The influence of different electroacupuncture strength between inflammatory cells factor and
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cerebral edema in cerebral ischemia reperfusion rats----------------Duan Lijun, Li HePing(204)277. The Research of Asthma patients with exhaled nitric oxide and blood eosinophil
levels----------------------------------------------------Yang Weijiang, Wang Nana, Li Fengsen(205)
278. The Study of IL-4 、 IFN-γ Gene Methylation of Allergic Rhinitis in Uygur, Han people-------------------------------------------------------------------Lou ZhongZhang, Gao Li(206)
279. The Study on the relationship between Cruor-fibrinolysis system and the HuiZhuoTanZu Syndrome of Coronary Heart Disease------------------------------Xie Xiaoliu, An Dongqing(206)
280. Unique performance like pleural effusion fatal huge mediastinal liposarcoma a case----------------------------------------------Zhou LinFu, Li Meimei, Zhang Shijiang, et al(207)
281. Winter Disease consumer Clinical evaluation of asthma paste the treatment of chronic obstructive pulmonary disease---------------Wu Ruohan, Bian YongJun, Li GuangXi, et al(208)
282. Xinjiang Uighur congenital clubfoot genepolymorphism Analysis---------Dong HaoJie, Lv FaMing(208)
The Sixth Part Other Medicine283. Original article Accuracy of 18F-FDG PET/CT for lymph node staging in non-small-cell lung
cancers----------------------------------- Liu BaoJun, Dong JingCheng, Xu Changqing, et al(209)284. A new trial for relief-oriented design of molecule from natural ginseng other than hot water
extraction--------------------------------------------- Nobuo YAMAGUCHI, Tsugia MURAYAMA, Masafumi NAKATO, et al(210)
285. Antibacterial Activities of Extracts and Constituents in the Rhizomes of Diploid and Tetraploid Gingers (Zingiber officinale Roscoe)--------------------------------Patar A, Tanaka N, Maeda H, et al(210)
286. Biodiversity: A link between traditional medicine and modern medicine-----------------Anake KIJJOA(211)
287. Characteristics of traditional acupuncture therapies in Japan, Korea and China---------------------------------------Kenji Kawakita, Yong-Suk Kim, XiaoPin L, et al(211)
288. Current Trends in Medical Education--------------------------------------Muhammad Aslam(212)289. Education Management in Faculty of Medicine in Kabul Medical University, Kabul,
Afghanistan----------------------------------------------------------------------Samad Omar(212)290. Effectiveness of Integrated Medical Curriculum in Pakistan: A Public Medical College
Experience-------Muhammad Saeed, Muhammad Iqbal Khan, Zeiyad Afzal Keyani, et al(213)291. Folk medicine in Turkey---------------------------K. Hüsnü Can BASER, Ekrem SEZIK(214)292. Influence of motor activity on health condition of students in Avicenna Tajik State Medical
University----------------------------------------------------------------------------Sagdieva Sh. (214)293. Innovative Strategies in Medical Education and Challenges faced in Establishing Gulf
Medical University in the Middle East- A Case Study------------------------Vinod Abraham(215)
294. Knowledge, Attitude and practice of medical ethics of faculty of a medical university, in Karachi, Pakistan----------------Zulfiqar Ali Shaikh, Masood Hameed Khan, Dur Muhammad Shaikh, et al(215)
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295. QOL assessment and proposed scale for evaluation of traditional medicines------------Nobuo Yamaguchi , Nurmuhammat Amat, Yimid Delitiat, et al(216)
296. Search for bioactive compounds to inhibit plant pathogenic organisms--------------Narong Singbuaraudom(216)
297. Secondary metabolites from a Türkish marine sponges------------------------------Konuklugil,B, Proksch,P, Gözcelioğlu,B, et al(217)
298. Study of Prevalence of Asthma in male workers of Leather Tanneries in Lahore, Pakistan ----------------------------------------------------------------------------Ali, Sajid, Shehzad, et al(217)
299. Some questions of the development of Uzbek public medicine and practical aspects of it on the public health of the population-----------------------------------------------------Khamraev(218)
300. YNSA as a new acupuncture treatment for pain killing---------------------Hegyi Gabriella(218)301. Prevalence of gout in Pakistan-------------------------------------------------------------- Sajid(219)302. Traditional Uighur Medicine theory of "KuWat" (energy) classification and its function
---------------------------------------------------------------------------------Tursun T，Kawuz R(219)303. Experience of «RAWAN» application at sick children with bronchial asthma in
Turkmenista--------------------------------------------------------------- Dmytriy Sebrebryakov(220)304. Clinical effecacy observation Uighur Medicine Preparetion Denar on the patients with viral
hepatitis with Cholestatic syndrome----------------------------------- Dmytriy Sebrebryakov(221)305. Epidemiological analysis of vitiligo according to traditional Uyghur medical theory and
western medicine----------------------------------------------------------Tuohuti K, Shawuer R(221)
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19
A-001Overview of Traditional Chinese Medicine
Dong Jingcheng 1*1Huashan Hospital, Fudan University, Institute of Traditional Chinese and modern medicine, 200040, Shanghai, China;
*Correspondence should be addressed to E-mail: [email protected]
1. Traditional Medicine: Traditional Medicine refers to a wide range of medical systems developed independently before modern medicine. It is a part different from the mainstream of modern medicine: confronted medicine. The definition issued by World Health Organization is: medicine that includes treatment, diagnosis and prevention of diseases or maintenance of body healthy, making use of drugs based on plants, animals and minerals and one or a variety of methods including psychotherapy, body therapies and other means developed in practice. Many ancient civilizations and peoples in the world have their own traditional medicines. 2. Traditional Chinese Medicine: Traditional Chinese medicine (TCM) is a medical system with unique theories that accumulated, summarized and gradually formed in the long-term medical care and life practice in China. Traditional Chinese medicine collectively refers to medicines of all nationalities in China, including national medicine such as Han medicine, Tibetan medicine, Mongolian medicine, Uygur medicine. In all traditional Chinese medicines, due to Han’s largest population, earliest text history, longer cultural record, abundant clinical practice and perfect traditional medical theory system, the influence of the Han medicine is most significant in China as well as in the world. After the 19th century, Western medicine was introduced to China and got popularity. Han medicine was called ‘Chinese medicine’, different from the ‘Western’ modern medicine. 3. General situation of various traditional medicines in China: 1) Traditional Chinese medicine collectively refers to as the traditional medicine of all nationalities in China, including the medicine of the Han medicine, Uygur medicine, Tibetan medicine, Mongolian medicine, Dai medicine, Hui medicine, Miao medicine, etc. They are often self-contained, characteristic and profound. Han Chinese medicine (TCM) is found on theoretical basis such as yin and yang theory and five elements theory and physiological and pathological basis such as organs and meridians, focusing on the overall concept and syndrome differentiation, diagnosing based on the combination of observation, listening, interrogation, and pulse-taking. TCM is also created eight methods of treatments, namely, sweating, vomiting, purgation, harmonizing, warming, clearing heat, dispersing masses, medicating according to prescription principles involving monarch, minister, assistant and guide. The theoretical basis of Uighur medicine is regarded as temperament of different body fluids. Uighur medicine has a deep understanding of human anatomy. In addition to the great attention of four diagnostic methods, the urine also set diagnostic methods for urine diagnosis, observation of stool and sputum diagnosis except for inspection. Tibetan medicine also considers yin and yang theory and five Elements theory as a theoretical basis. In addition to the four diagnostic methods, it focuses on urine diagnosis and is characteristic of syndrome differentiation. Tibetan medicine composes prescriptions according to character, taste and function of drugs and character and taste after digestion. Mongolian medicine is based on yin and yang, five elements and five yuan theories, and has a more profound understanding of human anatomy. In addition to commonly used observation, listening, interrogation, and pulse-taking, Mongolian medicine also focuses on other diagnostic methods such as press and olfaction as well as urine diagnosis and differentiation of cold and heat. Dai medicine has a thorough understanding of body structure. It pays attention to the differentiation of four elements and five skandhas and the differentiation of three pan with delicate
1
principals and fruitful methods. Dai medicine contains simple, small and generous prescriptions. Hui medicine is dominated by harmony between human being and nature and the idea of organic whole, and based on primordial qi, yin and yang and seven elements doctrines. It is predominant of differentiation of physiques, combined with differentiation of syndrome, disease and meridian. Hui medicine pays attention to syndrome differentiation, and therapeutic methods are flexible and abundant. Miao medicine classifies all diseases as two categories: cold and fever. Thermal treatment is used in cold disease and cooling treatment is used in fever disease. The methods of treatment are very rich. The characteristics of prescription are single distribution and trinity. Zhuang Medicine is oriented by yin and yang theory and regards three air synchronization view as the theoretical basis. It emphasizes on eye diagnosis, focusing on a combination of differentiation of disease and syndrome, attaching great importance to the use of animal drugs. The major traditional medicines are compared with respect to conception, formation time, philosophical foundation, representative celebrities, representative works, anatomy, physiology, disease name, etiology, pathogenesis, diagnosis, treatment principle, treatment method, prescription, medication characteristics, and it is easy to find the great similarity among them. 2) Traditional Chinese medicine is composed of the three parts: Chinese medicine, ethnic medicine and folk herbs. Before Opium War, Chinese medicine has always been the mainstream medicine in Chinese society. Both commonalities and differences exist in Chinese medicine and other national medicine. Ethnic medicines are independently creative and self-contained in their own culture, and they form their system respectively with characteristics. China has 55 minorities. From the ancient to the modern society, they have numerous creations in medication and only parts of them have been spread till now. Due to the uneven development of various ethnic medicines, there is significant difference in the ability of inheritance and propagation among them. Therefore, to the present, only Uighur, Tibetan, Mongolian, Dai, Miao medicine have established their teaching and research system. Understanding and application of traditional Chinese medicine is based on Chinese medicine theory, and has a unique theoretical system. The core of Chinese medicine theory is the nature of herbs, including four natures, five favors, channel tropism, ascending and descending, toxicity, etc. Similar to traditional Chinese medicine, Uighur, Tibetan, Mongolian, Dai, Miao and other ethnic medicines also have their own medicinal theory system. Uighur Medicine has ‘Alcorn doctrine’, ‘Mi Zha Ji Doctrine’, including their drug doctrines of herbs, animal drugs and mineral drugs. Character and taste of these herbs are divided into dry, hot, wet, cold and hot and dry, hot and humid, wet and cold, dry and cold, and 1, 2, 3, 4 classes. According to their nature, Tibetan medicine is divided into ‘hot’ and ‘cold’ categories, with six flavors, eight natures and seventeen kinds of effects. Tibetan medicine is able to treat 20 kinds of diseases. The basic nature and flavor of Mongolian medicine is sweet, sour, salty, bitter, acrid and astringent. According to ascending and descending, and formed herbal theory, eight effects of Mongolian medicine are summarized, including heavy, greasy, cold, blunt, light, blunt, hot and sharp, known as flavors of eight effects. Dai medicine contains doctrine of four elements and five skandhas . Internal treatment of Miao medicine emphasizes cold medicine for fever disease, hot medicine for cold disease, and reinforcing methods for deficiency syndrome. Flavors of fragrant, spicy, sweet, tingling are attributed to thermal drugs, and tastes of acid, pungent and astringent are ascription to cold medicine. Prescription is based on clinical experience. In different ethnic medicinal systems, there are similarities and differences for the same herb. In addition, long-term used medicinal plants in some minorities are named by their local names. 3) In summary, we find that: traditional medicines of all nationalities in China are full of intensive theory, flexibly diagnostic and therapeutic techniques, elegant prescription and medication. They have their own characteristics and systems, and make a great contribution for keeping Chinese
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prosperity. But we should also recognize that course of development of Chinese traditional medicines is quite different from modern medicine, it is always around the center of diagnosis, treatment and solution to practical problems and collects all the wisdom. By means of ancient plain materialist philosophy, operational and practical medical system are established. The core of the system is ‘organs theory’, ‘body fluid theory’, ‘temperament theory’, ‘material theory’ and etc. This system is full of technical science and empirical science, representing the highest establishment of experimental medicine in the world, and it is the result of the collective efforts made by Chinese ancestors in the field of traditional medicine. In this process, it is difficult to find out the hint of theoretical science, basic medical research and their contribution. A variety of traditional medicine build theory centered with viscera and body fluids, which reflected that people's understanding to human life are still at an early stage. In the view of theoretical value, there is little gap between viscera theory and body fluids theory. Both belong to the simple phenomenological theory, and have simple whole view, balanced view, etiological view, pathological view and preventive view. However, viscera theory is more specific and abundant than humoral theory. Just because of this, viscera theory is more practical than humoral theory. Viscera theory correlates the function of viscera and human external variables through intermediate variables such as gas, blood, sperm, body fluid, and ultimately reached by the state that inferring external manifestation by means of changing viscera function or identifying viscera function via external manifestation. Not only that, the determination of herbal efficacy are also included into unified system centered with viscera theory, making reason, method, compound and drug integrated. Since Chinese traditional medicine represented by Chinese medicine formed, experienced medicine in clinic was developed instead of experimental medicine and basic medicine; however, it has reached the highest realm of human experienced medicine. Some of these valuable experiences have been proved consistent with the knowledge of modern medicine; and some experiences are likely to contain certain future direction of the development of modern medicine; it is without doubt that valuable experiences born thousands of years ago have their temporal and cultural limitations. In this medical system, in addition to valuable experiences, there are a lot of understanding with temporal and cultural imprint. For example, in 1870, before human knew the relationship between microbes and diseases, all infection and diapyesis phenomenon will be generally described as ‘toxins’, ‘miasma’, ‘hostility’ and etc.; human’s corresponding disease resistance mechanism such as immune mechanism is generally referred to ‘righteousness’. Western historians LH·Banar said the following statement for the medical advances before 1878: ‘The medical science had not yet been prepared at that time. A large part of medical practice was medical technology and treatment of the disease was to reduce the symptoms rather than correct the pathological alternation. Laxative, bloodletting, extract, tincture and multi-drug mixture, the compositions in many parts appears to be only a little or no pharmacological effect from the current point of view, but they were general treatments at that time. For general health measures of disease prevention, they were just beginning to be noticed.’
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A-002The Basic Structure of Traditional Chinese Medicine
Dong Jingcheng 1*1Huashan Hospital, Fudan University, Institute of Traditional Chinese and modern medicine, 200040, Shanghai, China;
*Correspondence should be addressed to E-mail: [email protected]
1. Introduction: As mentioned earlier, the essence of the traditional Chinese medicine has always been most advanced experienced medicine in the world. Its vast system is full of practical medical technology and experiences that gradually recognized by modern medicine; it contains the germ that indicates the direction of future development of human medicine; still, it is widespread to find the simple understanding of temporal and special cultural imprint. Therefore, generally speaking, the basic structure of traditional Chinese medicine may be mainly composed of the following three parts: the part that is consistent with modern medicine, the part that is beyond modern medicine unconsciously and the part that needs to be re-recognized or abandoned. 2. The part that is consistent with modern medicine: In the 16th century, the so-called ‘western medicine’ was introduced to China, but had little effect. After the Opium War, ‘western medicine’ began to develop in China, and therefore in the history of Chinese civilization before the Opium War, traditional Chinese medicine had always been the leading force of medical care in the Chinese nation. The key of successfully medical are lies in its efficacy. ‘Excellence is from experience’. A lot of practice and experiences construct a solid foundation for its theory. After the foundation of new China, the development of ‘western medicine’ in China has entered into a new stage. With the increasingly deepened impact of modern medicine, especially the development of ‘integrative medicine’ from the end of 1950's, some theories, therapeutic principle, technology, prescriptions of modern life science were elaborated and the basic structure of traditional Chinese medicine also became clearly. One of the most important parts is the part that has reached a consensus with modern medicine, and that correlated with many aspects of basis and clinic in traditional Chinese medicine. 3. The part ahead of modern medicine unconsciously: Chinese traditional medicine is a practice medicine built on the basis of experiences. The theoretical system is built by means of ancient plain materialist philosophy, mostly by the method of ‘syndrome differenciation’, use of natural means to treat illness rather than emphasize on consistency with contemporary science and technology. Unlike modern medicine, the view that ‘Nature that cannot be certified cannot be conquered’, keeping abreast of requirements of contemporary science and technology, makes traditional Chinese medicine unconsciously have the super-awareness of the era in addition to the overall features of the old and simple, that is, the so-called "integrative research is able to continue to reveal the essence. 4. Some parts which need to be newly recognized or abandoned: In the system of human science, including medical science, the conception becomes from new to old while its correction becomes from absolute to relative, and the application extension becomes from large to small. It is the inevitable regulation of development. All the concepts and rules indicate the stable aspect of object. However, this kind of stableness is conditional, partial and relative while unstableness is absolute and unconditional. Any scientific systems should confess their demerits and rectify them so as to make new concepts, methods and theory. Meanwhile, modern medicine and Chinese Traditional Medicine were founded in some simple recognition and culture and specific era. So TCM theory should be divided into two parts, one of
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which need to be promoted, the other of which need to be abandoned. Integrative medicine is an important way to achieve it.
A-003The Future of Traditional Chinese Medicine
Dong Jingcheng 1*1Huashan Hospital, Fudan University, Institute of Traditional Chinese and modern medicine, 200040, Shanghai, China;
*Correspondence should be addressed to E-mail: [email protected]
We should scientificly and rationally recognize TCM, including its theory, experience, technology, method and prescription, define its merits and demerits by using modern technology and method like epidemic method and medical certificate method so as to further confirm scientificalness and efficacy. We should define the mechanism and material basis of modern science and develop the comparison of similar theory, technology, method and prescription, valuing survival of the fittest to make TCM pith outstanding. In addition, when study traditional Chinese medicine, especially drugs, we should pay attention to its basic ideas and be guided by these ideas, which will help the modernized development of traditional Chinese medicine. A large number of clinical evidence suggests that traditional Chinese medicine is of lower efficacy and potency than modern chemical drugs. One of the reasons for this phenomenon may be a departure from basic theories of traditional medicine in researching drugs, such as the whole idea; currently researches on traditional medicines are mostly carried out under the guidance of the reductionist thinking used in modern medicine. Take anti-arrhythmic drugs as an example, researches based on reductionist thinking indicate that Chinese medicine monomers such as berberine are of lower efficacy than modern drugs such as amiodarone in the treatment of arrhythmia. But under the guidance of the whole idea, the above-mentioned anti-arrhythmic medicine monomers are not only the best target of the disease, but have multi-target effects and intervene at least two ion channels. Clinically, if multiple monomers intervene with reasonably compatibility, satisfactory effects will be gotten and with no obvious side effects. However, treatment of synthetic drug with single component has an average effect but obvious side effects. For example, quinidine is a kind of arrhythmia drugs in modern medicine, but quinidine may induce Q-T interval syndrome and arrhythmia because it inhibits a certain target strongly. Thus, in order to develop safe and efficient antiarrhythmic drugs, we should study compounds, effective, distinguished and experienced prescriptions, analyze the effective components, and find out the best compatibility acted on multiple targets. In this way, we are able to develop new drugs at the component level. Essence speaking, the characteristic of traditional medicine is wholism which means the synthetic action of multi-elements, multi-targets, multi-angle and multi-layers to adjust the treatment of diseases. The basic medicine mechanism should be the basic point of synthetic action. When each herbal medicine in the prescription is clear, the effective combination to human should be studied so that the syndrome in TCM could be made clear. In other words, medicine tests syndrome. Some famous medicines of modern medicine come from ancient natural plants, whose developing experience should be a good way of many effective traditional medicines. The true value of integrative medicine and modernized traditional medicine is not the foundation of new theories, but the mechanisms of those effective traditional medicines, which enriches the culture of modern medicine. And it perhaps the historic mission of TCM and integrative medicine. ‘……There is a incline that we can forget the close combination of whole science and human being,
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forget those advanced and profound scientific findings. Leaving them alone is just meaningless.’ ——Oven Schrodinger﹒
A-004The Literature Research of Ming Dynasty Physicians on Treating the Syndrome of Chest Pain
Li Fangshuai1, An Dongqing1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: Collecting and collating the main medical writings about chest pain’s
etiology，pathogenesis and prescriptions in Ming Dynasty, discussing the understanding of the etiology
and pathogenesis when Ming Dynasty physicians treating chest pain, summarizing the characteristics and laws of using medicine of this period in order to better serve and clinical work. Methods: 1.The method of traditional philology, mathematical statistics and using the prescription to prove syndrome, to discuss the law of prescription for treating chest pain from relevant books written by 22 doctors in Ming Dynasty. And select the etiology and pathogenesis of Wang Kentang, Liu Chun, Qin Jingming works for chest pain, prescription summary, the induction of a common illness machines and the law. Results: The traditional Chinese medicine treatment of chest pain from Ming Dynasty physician: use 142 kinds of medicine totally, cumulative 954 times less frequent dosing. Most of the herbs are for tonifying deficiency, moving blood, warming organs and smoothing qi, the cumulative frequency is 64.89%; the frequency of using it is more than 1%, the 24 taste of commonly used herb is licorice, orange peel, ginseng, cinnamon, Wulingzhi etc, the cumulative frequency is 55.45%; Prescription herbs of Siqi is mainly 68% warm and calm; bitter, acrid, sweet of Wuwei mainly accounted for 95.8%; Spleen, heart and liver meridian is mainly 61%; Frequency greater than 1% of the 24 herbal medicine in the cluster analysis, automatic formation of the seven cluster combinations. 2.Three on behalf of the physicians (Wang ken tang, Liu Chun, Qin jing ming) write on Chest Pain etiology of pathogenic cold invasion and occupation (32.31%), physical weakness (20%), due to pathogenic heat (18.46%), emotional chastity and eating disorders (10.77%). Medication usage: the prescription of tonifying deficiency, moving blood, warming organs and smoothing qi, the most commonly used cumulative frequency is 55.15%. There is no significant difference in Wuwei and meridian belongs of herb using from three doctors prescription, there are some differences in the use of four character herbs. Conclusion: (1) The prescription law of Ming Dynasty physicians is: Commonly used herbs is for tonifying deficiency, moving blood, warming① organs and smoothing qi, Besides commonly used supplementary herbs such as licorice and ginger,② Wulingzhi and ginseng as main and effective herb on behalf of the physicians in the Ming Dynasty to treat chest pain, Siqi is mainly warm and calm, bitter, acrid, sweet of Wuwei accounting for the main,③ the main meridian are spleen, heart and liver meridian; (2) The method of using the prescription to prove syndrome is mainly qi and yin deficiency, blood stagnation syndrome, qi stagnation and cardiothoracic, cold stagnation in systolic, which is commonly seen. (3) the physician of the Ming Dynasty believe that the chest pain is related to cold evil invasion, physical weakness, heat evil, emotional chastity, eating disorders, exerting internal injuries and other factors, the main pathogenesis is systolic Blockage.
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A-005The Preliminary Studies on Herbal Medicine Resources of Aconitum from Ghulja and Easten Region in Xinjiang
Liu Shumin1, Nie Jihong1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: Initially to investigate the species distribution, habitat and reserve situation of Xinjiang Aconitum plant. To detect the determination of Xinjiang Aconitum Plant Alkaloids and diester alkaloid content, to certify the resources of the chemical composition. Adopting TLC fingerprinting method, preliminaryly to carry out the similarity comparison, cluster analysis and principal component analysis of Aconitum plants in Xinjiang. Initially to select the species of potential medicinal value and similarity with the existing national legal herbs standard Aconitum carmichaelii Debx. And Aconitum kusnezoffii Reichb, which is widely distributed and abundantly reserved form Xinjiang Aconitum species. To provide scientific basis for rational using and development of Aconitum species. Methods: Reviewed literature and specimens, surveyed species distribution, habitat and status of reserves of Xinjiang Aconitum plant. Taking Aconitine as reference, determined the total alkaloid content in the Xinjiang Aconitum plants by UV spectropHotometers with acid dyes. Determined aconitine hypaconitine Aconitine in the Xinjiang Aconitum plants by HPLC. Using high performance thin layer silica gel G plate, taking n-hexane - ethyl acetate-methanol(6.4:3.6:1) for dilute potassium iodide, show color with thin bismuth iodide solution of potassium, got thin layer of fingerprint with color, to generate common pattern via the chemical fingerprint analysis system software, and carryed out similarity analysis, cluster analysis and principal component analysis. Results: Initially found five kinds of Xinjiang Aconitum medicinal plants, of which Aconitum leucostomum Worosch. and Aconitum soongaricum Stapf. in Ghulja were rich, and worthy of further development and utilization. UV spectropHotometric determined the aconitine between the concentration range of 0.01176~0.04704 mg/mL had a good linear relationship, the regression equation A=16.484 C+0.0592，r =0.9992. Average recovery was 100.19%, RSD=1.33%. Sample assay results showed that total alkaloid content of the Xinjiang Aconitum species is similar with the existing legal herbs standard Aconitum carmichaelii Debx. and Aconitum kusnezoffii Reichb., it was worthy of further studying. By HPLC mesaconitine had a good linear relationship (r =0.9999) between 0.0022~0.0864 mg/mL, the average recovery was 99.69%, RSD=1.04%; aconitine had a good linear relationship (r=0.9999) between 0.0201~2.0120 mg/mL, average recovery was 100.06%, RSD=0.60%; hypaconitine had a good linear relationship (r=0.9999) between 0.0021~0.0840 mg/mL, the average recovery was 99.68%, RSD=1.08%. Sample assay results showed that Xinjiang Aconitum plants contained diester alkaloids, but the content and ingredients were different between different kinds. Screened and optimized the conditions for thin layer chromatograpHy, spots were clear and separation was good; similarity analysis showed that Xinjiang Aconitum had some differences in chemical composition between different plant species, which can be broadly divided into three categories. Among which
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aconitum leucostomum Worosch. and Aconitum apetalum(Huth) B. Fedtsch. Had high similarity with Aconitum carmichaelii Debx. And Aconitum kusnezoffii Reichb. Conclusion: Xinjiang Aconitum species had high medicinal value, and deserved for further studied. UV spectropHotometry was simple, accurate, and can be used for the determination of total alkaloids from Xinjiang Aconitum plants. HPLC method was simple, accurate, high sensitivity, can be used in determination of the diester alkaloids from Xinjiang Aconitum plants. Established thin-layer chromatograpHic fingerprints laid the methodological foundation for the study of the fingerprint of Xinjiang Aconitum plants. Initially selected the species of Aconitum leucostomum Worosch. and Aconitum soongaricum Stapf., which were widely distributed and abundantly reserved form Xinjiang Aconitum species, and had potential medicinal value and similar with the existed national legal herbs standard, were worthy of further being studied.
A-006A Systematic Review on Zhisousan Treatment of Infections
Li Fengsen 1*, Jing Jing1, Li Chunyan1, Gao Zhen 1,2
1 Affiliated Traditional Chinese Medicine Hospital，Xinjiang Medical University& National Clinical Research Base of Traditional Chinese Medicine, 830000, Urumqi, Xinjiang, China; 2Xinjiang medical university, Urumqi, 830011, Xinjiang, China;
*Correspondence should be addressed to [email protected]
Objective: A systematic review on Zhisousan treatment of infections. Methods: Meta-analysis of randomized controlled trial (RCT) cough Decoction combined with Western medicine to treat the infection, to adopt RevMan5.1 software for statistical analysis. Results: A total of 24 published RCT (n = 2 274), but the prevalence of lack of methodological; Cough simply scattered its addition and subtraction with western medicine is efficient compared to fixed effects models show that combined OR = 7.90, 95% CI [5.67,11.01], P <0.00001; Zhisousan and Decoction combined with Western medicine. western medicine is efficient compared to fixed effects models show that the merger, OR = 3.62,95% CI [2.22,5.92], P <0.00001. Conclusion: Zhisousan Treating infections cough may be effective, but because research and test methodology flawed, and there is publication bias, the findings still can not fully sure, we still need high-quality research trials further confirmed.
A-007The clinical study on Side-lying position and shaking method in treating Cervical Spondylotic Radiculopathy of 120 Cases
Liu Junchang1, Muhetaer Ayoufu1, Wang Xinjun1* 1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: To compare the curative effects of cervical spondylosis after treatment by “Side-lying position bowed their heads and shaking method”. Methods: 120 subjects were randomized into two groups: treatment group in which 60 cases were treated by Side-lying position bowed their heads and shaking method and control group in which 60 cases were treated by Seat inclined pull method, once every other day were treated 10 times. The clinical symptoms and their scores were observed. Results:
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After treatment, the clinical symptoms were all improving in both groups(P＜0.01),with better effects in treatment group than in control group in improving neck and shoulder pain and cervical movement（P＜ 0.05） . Conclusion: The “Side-lying position bowed their heads and shaking method” is quite effetive for cervical Spondylotic radiculopathy, and is worth promoting.
A-008Body Constitution Classification (BCC) of Coronary Heart Disease (CHD) in some parts of Xinjiang
Li Hui1, An Dongqing1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: to explore the east, south, north of body constitution features of TCM patients with coronary artery disease, to get coronary artery disease and providing the theoretic basis. Methods: From October 2009 to October 2010, using cross-sectional survey method, in the east, south, north the selected five municipal districts and 10 hospitals with coronary heart disease was the first collected by western medicine diagnosis and diagnosis of coronary heart disease and TCM diagnosis for chest discomfort in 660 examples, using standardized 9 kinds of TCM physique scale in patients with coronary artery disease, TCM identification from the physical implementation of gender, age, regional, national, body mass index, syndrome type, coronary lesions count explore characteristics. Results: (1) the east, south, north of TCM type of constitution in patients with coronary artery disease and clip physique, false or true inclusion type of constitution is given priority to, Xinjiang patients with coronary artery disease common TCM type of constitution for qualitative, phlegmy wet qi difficiency and blood stasis qualitative; qualitative； (2) different sexes, different age, body mass index, national physique different characteristics；(3) shouts turbidity phlegm resistance syndrome patients with and clip biased physique, false or true constitution is given priority to, with the inclusion uncleanness turbidity phlegm resistance syndrome patients constitution comparison was statistically significant (P<0.05)； (4) this survey altogether collected 660 of coronary heart disease patients, choose a case of coronary angiography results of total 249, each type of constitution in coronary lesions narrow count comparison, was not statistically significant (P>0.05), shouts turbidity phlegm resistance syndrome group with the uncleanness turbidity phlegm resistance syndrome group coronary lesions count comparison, multivessel disease comparison was statistically significant (P<0.05).Conclusion: this study found that patients with coronary artery disease TCM type of body constitution in xinjiang with qualitative, phlegmy wet qi difficiency and blood stasis qualitative qualitative, in each type of constitution proportion in patients with coronary artery disease is higher, it is main type of constitution, and concurrently clip physique, false or true mingled with physical phenomenon exists generally, Xinjiang patients with coronary artery disease syndrome differentiations with shouts turbidity phlegm resistance syndrome is given priority to, deficiency of qualitative, phlegmy wet qualitative, blood stasis quality physique patients are predisposed to coronary heart disease and easier to develop into shouts turbidity phlegm resistance syndrome, Different sexes, ages, regional, national, weight, syndrome type, degree of coronary lesions TCM body characteristics different, and the results indicated that the above three physical may in coronary heart disease occurrence, development exists some internal relevance.
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A-009Case-control Study of the Association between Northwest Dryness Syndrome and Chronic gastritis
Zhang Huitian1, Zhou Mingxin1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to zmx9998@ vip.sina. com
Objective: To investigate the Northwest Dryness Syndrome (NDS) and chronic gastritis, starting from the NDS to provide prevention and treatment of chronic gastritis according to the Xinjiang region. Methods: Epidemiological case-control study by modern methods, Questionnaires as the main form ， according to the theory of traditional Chinese medicine syndromes of disease and its comprehensive analysis of the performance. Results:(1)Compared with other parts of the country, Xinjiang chronic gastritis symptoms and syndrome type has its own characteristics;(2) Case group NDS attack rate was 37.86%, in the control group NDS attack rate was14.29%, the difference was statistically significant (P <0.01);(3) Case group and control group compare points of NDS, Lung , skin and opening of the body syndrome , deficiencies of essence and blood of the liver and t he kidney syndrome ,yin deficiencies of the heart and kidney syndrome, yin deficiencies of the lien and stomach syndrome, and endogenous dampness syndrome, the differences were statistically significant (P <0.05); (4) Dryness in the case group and non-NDS group set TCM syndromes compared, Fan Wei liver, spleen Deficiency, damp heat resistance, yin deficiency of stomach, diet Shang Wei and cold Fan Wei card, the difference was statistically significant (P <0.05);(5) The total deficiency on the incidence, the dry card cases and cases of non-dry group, dryness control group, non-dry the control group, statistically significant differences (P <0.05); Comparison of the total deficiency points, Dryness cases and cases of non-dry group, NDS of case group and control group, NDS case group and control group of non-dry, non-dry case group and control group of non-dry, dryness control group and the control group of non-dry, the difference was statistically significant (P <0.05). Conclusion: The impact of the NDS, the Xinjiang region is different from other regions of chronic gastritis, has its own characteristics; Patients with chronic gastritis and the prevalence NDS card points are higher than normal intelligence; Patients with chronic gastritis were diagnosed NDS risk of illness than those who have none. To the total deficiency, the chronic gastritis combined deficiency in patients with NDS and gas, blood, yin, yang permit incidence dryness were higher than those who suffer and have more disease who suffer from more non-NDS Serious.
A-010The Effect of dipeptidyl peptidase IV in 3 extracts of plants from Xinjiang
Yang Zhenzhen1, Wang Xuehua2, Wang Ye1, Mao Xinmin3, Han Xue1, Yi Jinyang1, Wang Lifeng4, Ma Xiaoli5, Li Linlin1*1Department of Pharmacology, Xinjiang Medical University, Urumqi 830011, China; Traditional Chinese Medicine Institute,
Urumqi 830011, China; 4 Department of Physiology, Xinjiang Medical University, Urumqi 830011, China; 5 Department of
Analysis and Testing Center Xinjiang Medical University, Urumqi 830011, China; 2Xinyuan People's Hospital of Xinjing Yili
835800, China; * Correspondence should be addressed to [email protected]
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Objective: To study the effect of dipeptidyl peptidase IV in extracts from Trigonella foenum-graecum L. Cicer arietinum L. Punica Granatum L. Methods: The inhibitory effects of these 3 extracts to dipeptidyl peptidase IV activity were investigated by in vitro enzymatic method and IIe-Pro-IIe was used as positive reference substance. Comparison of 3 kinds of water extracts of IC50. Results: IIe-pro-IIe IC50 is 0.01 mg/mL. Trigonella foenum-graecum L. Water extraction IC50 is 0.03mg/mL, Punica Granatum L. water extraction IC50 is 0.19 mg/mL, Cicer arietinum L. water extraction IC50 is 0.09 mg/mL.Conclusion: The 3 extracts of Xin Jiang antidiabetic plant showed obviously inhibitory action against dipeptidyl peptidase IV activity. Trigonella、 Pomegranate husk、 Cicer arinum L. water extraction to dipeptidyl peptidase IV have different degrees of inhibition.
A-011The Effects of TianXiangDan on Blood Lipid and Vascular Endothelial Growth Factor in the Arteriosclerosis in ApoE (-/-) gene Knockout Mice
Jiao Yujie1, An Dongqing1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: To observe the TianXiangDan on apolipoprotein E gene knockout (ApoE (-/-)) mouse in atherosclerosis (Arteriosclerosis AS) of vascular endothelial growth factor (vascular endothelial growth factor ,VEGF)and blood lipid effects. Method：Sixty Eight-week old ApoE(-/-)gene knockout mice were randomly devided into 6 groups : control group(fed with 0.9%NACL0.2ml·d-1·10g-1， n=10),model group(fed with0.9%NACL 0.2ml·d-1·10g-1， n=10),TianXiangDan (TXD) high dose group(fed with TianXiangDan 0.36g/mL,n=10), TianXiangDan (TXD) medium dose group(fed with TianXiangDan 0.18g/mL,n=10), low dose group(fed with TianXiangDan 0.09g/mL,n=10), and Atorvastatin Calcium Tablets (ATV) group (fed with ATV 0.2mg/mL,n=10). The mice were sacrificed after 12 weeks． The lesion of atherosclerosis was observed by microscope and pathological staining， respectively. The Plasma level of blood lipid was measured by test kit, the level of VEGF was measured by ELISA kit. Results：microscope and hematoxylinenosin staining showed that the area of atherosclerotic lesion was significantly less in TXD-treated groups than in model group(P<0.05),especially TXD high dose group was the lest.The result of aortic arch stained showed lipid deposition was less in TXD-treated groups mice than in the model group(P<0.05), also especially TXD high dose group was the lest. Moreover ， the level of VEGF was lower in TXD-treated groups mice than in the model group(P<0.05)．also especially TXD high dose group was lowest. Conclusion：TianXiangDan could reduce the levels of serum total cholesterol (Serum total cholesterol TC), triglyceride(Triglyceride TG) and low-density lipoprotein cholesterol(Low density lipoprotein LDL-C) on apolipoprotein E-knockout mice . TianXiangDan could reduce plaque of peripheral atherosclerosis in VEGF expression and the area of AS plaque effects.
A-012Primary Study on Chemical Composition and Biological Activity of Arctium tomentosum
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Mill
Zhang Haoke1, Tian Shuge1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: By studying on chemical composition and its antioxidant, antibacterial effect, to provide research data pharmacological effect further study and reasonable exploitation and utilization of Arctium tomentosum Mill. Methods: (1) Study the extraction process of arctiin in Arctium tomentosum Mill. through extraction method, extraction solvent and its consumption separately, then choose the best extraction process. (2) Determine arctiin and arctigenin in different extraction parts by HPLC to define enrichment parts of arctiin. (3) Separate and purify the ethanol extract to get arctiin monomer. (4) Apply two antioxidant models (remove DPPH free radicals, the vitality effect superoxide anion) to select antioxidant activity from different polar extraction parts of ethanol extractions. (5) Use 96-well plates to select antibacterial activity from different polar extraction parts of ethanol extractions. Results: (1) The best extraction process of arctiin Arctium tomentosum Mill. using 70% ethanol reflux extraction for 1h, 8 times solvent and extract 3 times. (2) The root of Arctium tomentosum Mill. contain a little arctiin and no arctigenin; compared with other parts dry and mature seeds contain more arctiin and arctigenin; there are little arctigenin but no arctiin in leaf and flower; the stem and petiole contain almost no arctiin and arctigenin. (3) Study the chemical composition of Arctium tomentosum Mill. by spectroscopy(1H-NMR、13C-NMR、IR、UV) and define it is arctiin. (4) Antioxidant activity experiment proved that ethyl acetate extraction part has good antioxidant activity. (5) Butanol extraction part has good activity of antibacterial and inhibit the growth of bacterial biofilm. Conclusions: (1) The ethanol extraction parts of Arctium tomentosum Mill mainly contains lignans and high content of arctiin. (2) Established the methods of extraction, separation, purification for Arctium tomentosum Mill. which are simple and stable. (3) Ethyl acetate extraction part has good antioxidant activity. (4) Butanol extraction part has good antibacterial activity.
A-013The Study on the Quantities Chemical Composition and Chemical Pattern Recognition in Coriopsis Ticntoria from Different Areas
Sun Zizeng1, Zhang Yanli1, Aytulun Simayil1*1Pharmacuetical Institute, Xinjiang Medical University, Urumqi 830011, China; *Correspondence should be addressed to
Simayil A
Objective: To study the character of the contents and distribution of chemical composition of Uygur Medicine Coreopsis ticntoria flowers (CTF), and to provide valuable experimental evidence for the quality evaluation and control of CTF. Method: Ten batches of samples，CTF．coming from different producing areas of Xinjiang were carried out to quantitate by UV and ICP． Principal component analysis and Hierarchical clustering analysis were applied to the study on the chemical components which were extracted from the original data. The principal component composition and chemical pattern recognition．Result: Three principal analysis results showed that Cu, Zn，Fe，Mn，Ni，Cr and Al may be the characteristic elements. The ten samples could be clustered reasonably into three groups,
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and the contents of chemical composition of CTF were related to the ecological environment, such as temperature and location．Conclusion: This paper, by measuring the number of content indicators, using principal component analysis and cluster analysis clearly reveals the regularity of the different origins and differences in CTF．
A-014Regulation Effect of OboBiyanChongji on Expression of IL-2, IFN-γ, IL-13 and Nasal Mucosa in the Experimental Allergic Rhinitis in Rats
Jiang Liming1, An Yang1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: To investigate the effect of OboBiyanChongji for allergic rhinitis (AR) rats on IL-2, IFN-γ, IL-13, IgE levels and nasal mucosa, and compare OboBiyanChongji groups with XinQin particles groups to indentify the mechanism of pharmacodynamic action and theropy best dose. Method: Egg albumin sensitz- ation made rats animal model of allergic rhinitis. The model successfully rats were treated with different doses of OboBiyanChongji, saline, Xin Qin particles, which are compared with each other. The method of ELISA determine in serum the total IL-2, IFN–γ, IL-13 and IgE level. Using Giemsa staining for detection of nasal mucosal eosinophilia. Calculate the nasal mucosa and submucosal eosinophilic number by using microscope. Results: The results of serum IL-2, IFN-γ of high dose group are significantly different with the model group, low dose group , middle dose group, nasal eosinophilic expression (P<0.05). The levels of IL-13, IgE and nasal mucosal eosinophilic expression decreased (P<0.05). The high dose group and blank group are relative to plasma in IFN-γ, IL-2, IL-13, IgE and eosinophilic expression differ significantly (P<0.05). But they has no statistical significance with medium dose group (P>0.05). Conclusion: High dose of OboBiyanChongji can effectively improve the allergic rhinitis in rat plasma IL-2, IFN-γ levels, and decrease IL-13, IgE and nasal mucosal eosinophil levels. The high dose group of OboBiyanChongji is more effective than low doses, middle group and XinQin particle group.
A-015Research on the Changes of Different Contentsin Fried Aconitum pendulum Busch
Wu Lin1, Zhang Fan1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objectives: To analyze the influences on contents of toxic and active compounds in Aconitum pendulum Busch caused by different fried methods which were used by different national medicines, and to provide scientific basis for searching out the frying mechanism and enhance the quality of medicines. Methods: Several Aconitum pendulum Busch were fried by different special methods used in Tibetan medicine, Qiang medicine and Hui medicine. The total alkaloids in all fried products were isolated by ultrasonic wave method and HPLC method and ester alkaloid in all fried sample. ICP-AES, AAS and AFS were the first methods to be used to scan and analyze macro elements, trace elements
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and heavy metals in Aconitum pendulum Busch different fried samples, which were correlated with people’s health and lives. Results: All the contents of total alkaloid and ester alkaloid in Aconitum pendulum Busch samples decreased after fried. The method of frying with water could drop the toxicity largely, and the method of frying with Qingke wine may retain the activity of the medicine. All the heating methods were the deeply influencing to aconitum alkaloid. Also, it was found to be the content of Hg rose greatly in the experiment. Conclusion: Different methods of frying would influence the contents in Aconitum pendulum Busch.
A-016Research on Xinjiang Region Physical Types of Patients with Hyperuricemia on behalf of Teaand Chinese Medicine Intervention
Li Li1, An Dongqing1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: The incidence of hyperuricemia in recent years was significantly elevated, and concomitant cardiovascular disease are obviously increased, the onset of many influence factors of hyperuricemia and cardiovascular diseases, the relationship and related mechanism is complicated, many academic point of view remains controversial, this research through Xinjiang patients with traditional Chinese medicine constitution identification, discuss its physical type distribution clinical observation of traditional Chinese medicine, and through the generation of tea on patients with hyperuricemia uric acid, lipid metabolism index, renal excretory function and tissue plasminogen inhibitor effect of Chinese medicine on behalf of the tea, mechanism of action, for the early treatment of Chinese herbal medicine of hyperuricemia and concurrent disease and provide a theoretical basis. Methods: using the standard 9 kinds of TCM constitution classification in scale to 1400 patients with hyperuricemia patients constitution identification, create database. And choose from 70 patients with blood stasis syndrome type of phlegm dampness constitution hyperuricemia patients, randomized control principle is divided into treatment group and control group, two groups were given diet and exercise intervention, treatment of traditional Chinese medicine intervention group to make tea, observation curative effects after the treatment of integral, and blood uric acid (SUA) , lipid metabolism indices, renal excretory function and tissue plasminogen inhibitor (PAI-1) was analyzed by comparative analysis. Results: (1)Patients with hyperuricemia biased constitution mainly by the phlegm wet, damp heat and blood stasis quality 3 kinds of physical endowment. In the improvement of traditional Chinese medicine, traditional Chinese medicine on behalf of tea group superior efficacy to exercise control group (P<0.05) ; (2) two groups of intervention can significantly decrease the SUA (P<0.01); (3) improvement in TC,TG, traditional Chinese medicine on behalf of tea group was significantly better than the exercise control group (P<0. 01) ; (4) Two groups of PAI-1 more before treatment significantly (P < 0.01). Conclusion: Xinjiang region high uric acid of hematic disease patient biased constitution mainly phlegmy wet quality, hot and humid quality and blood stasis quality mainly three kinds of type of constitution, at the same time, the mountain tea can greatly improve phlegmy wet clip stasis syndrome in patients with high uric acid constitution disease symptoms of traditional Chinese medicine, can promote uric acid eduction, lipid metabolism disorders of has a therapeutic effect, after treatment, the organization type fibrinolytic enzyme inhibitors of the original (PAI-1)have declined obviously, and show that the tea mountain of high
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uric acid and related disease concurrent disease have certain preventive therapy effect.
A-017Safety Evaluation of Awei Capsule: Research of Acute and Subchronic Toxicity in rodent
Han Rong1, Xue Jie2*, Nurmuhammat Amat3*1Second Affiliation Hospital of Xinjiang Medical University, Urumqi , 830011, PRC； 2Research Department, Xinjiang
Medical University, Urumqi, 830011, PRC； 3Traditional Uighur Medicine Institute, Xinjiang Medical University, 393 Medical
University Road, 830011, Urumqi , Xinjiang Uighur Autonomous Region, China；*Correspondence should be addressed to
[email protected](Xuejie), [email protected].( Nurmuhammat.Amat)
Objective：Awei Capsule (AC) which is a medicinal herb formula has been used in traditional Chinese medicine in the treratment of diseases including and diabetes mellitus. However, there is very little information regarding the toxicity of CA to give an assurance of safety for clinical treatment. To provide safety information for CA, we evaluated the acute and chronic toxicity of the AC in rodent. Methods:For the acute study, ICR mice was administered in single doses of 56.9g/kg of AC given by gavage. Also determined the maximum tolerated dose (MTD) was the AC which was administered to ICR mice at doses of 56.9g /kg twice a day. General behavior adverse effects and mortality were determined for up to 14 days. In the sub-chronic dose study, the AC was administered rectally at doses of 2.3, 15.76 and 25.6g/kg daily for 180 consecutive days and the toxic effects were assessed using biochemical and haematological data and histology of vital organs. Results: We found no mortality and no abnormalities in clinical signs, body weights, and necropsy findings for any of the animals in the acute and subchronic studies. AC may not have any single-dose toxicity;the MTD of CA was over 9.888g/kg, and it is safe for mice. Conclusion:The results indicate that AC do not posses any significant acute toxicity in mice and chronic toxicity in rats.
A-018Sophora alopecuroides extract on human coloncancer cell line HCT116 strains of chick embryo chorioallantoic membrane experimental study on tumor angiogenesis
Li JingJie1, Lv Shuqin 1, 2*1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University；2Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should be addressed to [email protected]
Objective: Through the establishment of the people HCT116 colon cancer cell lines chicken embryos allantoic membrane transplantation tumor model, to observe Xinjiang bitter beans extract huai set after transplantation of alkali role of anti-angiogenic phenomenon, and observe xinjiang bitter beans thick total alkaloid in the role of colon cancer cells HCT116 people antiproliferative and induce the apoptosis of function, this paper discusses the xinjiang bitter beans antitumor, and the mechanism of action of angiogenesis. Method: By MTT method, flow cytometry detection of sophora alopecuroides Xinjiang was rough total alkaloid against human colon cancer cell proliferation HCT116 and induce the apoptosis role, and observe chicken embryos fluffy allantoic membrane transplantation tumor angiogenesis changes, with the positive buy fluorescent microscope the ratio of blood vessels; Immunohistochemical method to determine the chicken embryos fluffy allantoic membrane transplantation tumors VEGF
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dyeing strength and the change of MVD. Result: Xinjiang bitter beans thick total alkaloid colon cancer cells to HCT116 role 24, 48 h IC50 alue concentration were 24 mg/ml, 6.82 mg/ml. Xinjiang bitter beans thick total alkaloid can reduce people HCT116 colon cancer cell cycle G1 phase percentage, heighten S phase and can induce people HCT116 colon cancer cell apoptosis. Bitter beans extract set in the role of CAM tree alkali medicine group than blood vessels after area (VA/CAM) were less than model control group (P < 0.05), and at the same time, reduce tree set of the tumor, reduce the expression of VEGF microessel-density (P < 0.05). Conclusion: Xinjiang bitter beans thick total alkaloid can restrain people HCT116 colon cancer cell proliferation, and induce the apoptosis, interfere with the cell cycle G1 and S phase, which block the tumor cell mitosis. Bitter beans extract set in the role of alkali tree chicken embryos fluffy allantoic membrane transplantation tumor has obvious antiangiogenic effect, its mechanism may reduce the expression with tumors VEGF and reduce microessel-density relevant.
A-019Study on the difference between Uygur ethnic group and Han people who suffered from mild cognitive impairment (MCI) with electroacupuncture therapy
Guo Hui1, Liu Zhiyan1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
With the help of 1H-MRS to observe the clinical effect of electroacupuncture on mild cognitive impairment of the Uygur ethnic group and Han people, we researched into the diversity of nation differences between Uygur ethnic group and Han people of MCI, meanwhile, find the effective way to treat MCI by traditional Uygur Medicine. Divided 22 patients into Han people group (13 cases) and Uygur ethnic group (9 cases) according to the nation, based on the principle“tonifying kidney、replenishing essence and benefiting marrow, strengthing brain and benefiting mind , Take Bai hui、Feng chi、Xuan zhong、Fu liu、San yin jiao、Tai xi as the main acupoints, all of them received electroacupuncture, observe the change of MMSE 、 MOCA assess chart and two groups’ NAA/Cr、Cho/Cr before and after the treatment by 1H-MRS. There is an obvious difference on left side hippocampus (NAA/Cr) between Uygur ethnic group and Han people group compared with the treatment before. And the MMSE and MOCA changed obviously after three courses treatment compared with the treatment before (P<0.01)；NAA/Cr of Uygur ethnic group and Han people group’s left side hippocampus increased compared with the treatment before (P<0.01), Cho/Cr of Han people group’s left side hippocampus decreased compared with the treatment before (P<0.01), there is no obvious change on Uygur ethnic group ’s left side hippocampus (P>0.05). Electroacupuncture can improve the general cognitive function of mild cognitive impairment (MCI) patients; there may be nation diversity of Uygur ethnic group and Han people group mild cognitive impairment patients in the partial brain metabolite. The two groups patient’s NAA/Cr increased after treatment, Electroacupuncture could improve cognitive function and defer senility through repair nerve cell. The Han groups’ Cho/Cr decreased after treatment, Electroacupuncture could improve cognitive function and defer senility through restrain the form of neurospongium.
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A-020Study on the Pharmacognosy, Chemistry and Acute Toxicity of Aconitum Soongaricum Stapf
Cai Dongmei1, Zhang Fan1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: To study the pharmacognosy of Aconitum soongaricum Stapf systematically; to confirm the components and to study the acute toxicity of Aconitum soongaricum Stapf on mice, while investigating the acute toxicity, toxic signs, organ coefficient, median lethal dose (LD50) and 95% confidence interval on mice of A. soongaricum Stapf. Methods: Macroscopic and microscopic identifications were carried out to observe the crude drug, fabric and powder, and high performance liquid chromatography (HPLC) and thin layer chromatograph (TLC) were used to analyze the alkaloids in A. soongaricum Stapf, and corresponding methods were set up to determine the contents of the alkaloids. Different concentrations of A. soongaricum Stapf original medicinal powder suspension are given to mice, for observing and recording the activity and toxicity in mice, and at the same time, for computing the value of LD50. Results: The characteristics of A. soongaricum Stapf were spindle root, 2-4 roots, black brown skin and bluish violet sepals. The components of A. soongaricum Stapf were 12-epi-napelline, songorine, neoline, songoramine and napelline, and also, songoramine and napelline were found in it for the first time. The mice showed reduced activity, sweating, tremor and mouth breathing toxic symptoms after medicine administration about thirty min, and the peak mortality rate was two hours. The median lethal dose was 155 mg/kg, and the 95% confidence interval was 137-175 mg/kg. Conclusion: The results can be taken as the reference for identifying the species, analyzing the quality and laying down quality standard of the crude drug.
A-021Study on The Best Extraction With A ngeLica From Knee and ArthraLgia HerbaL Paste
S.Yun 1, G.Liang 1, W. Xinjun 1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: Study on the best extraction with angeLica from Knee and arthra l gia herbaL paste. Methods: The extraction technique was designed by orthogonal design, the content of Ferules acid as indicators, exploring the influence of factors such as ethanol concentration, ethanol volume, extraction time and so on. Results: Extraction of ferules acid in Angelica the optimum condition was: 50% to 7 times the amount of ethanol refluxing extract three times, 2.5h each time. Conclusion:The repeated experimental results are stable, and the method is feasible.
A-022TCM Hung Drag line Drainage is the Treatment of High Anal Fistula
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Chang Zhongsheng 1, Yu Dandan1
1 Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University First, 830054, Urumqi, China
O bjective : Explore Chinese medicine dragging line method for high anal fistula. In order to explore a more ideal operation method, we use Chinese medicine dragging line method about the treatment of 40 patients with high complexity of anal. Effectively improved the curative rate, Reduce the recurrence, panful, and shorten the course of treatment. Compared for the traditional hang line method, Chinese medicine dragging line method treatments of 40 patients with high complexity of anal were all cured. They anal physiological function, Position are all normal, no significantly damaged, no mucous leakage, no anal incontinence and form anal fistula ect. Conclusion: Chinese medicine dragging line method is an effective method for high anal fistula, it’s easy to operate, more security, and effective in preventing the occurrence of anal.
A-023The Effect of Sophora Alopecuroides Extract to the Receptor Tyrosine Kinases in Colorectal Cancer Cell Lines
Jiang Qin 1, lv ShuQin 1,2*1 Affilated Traditional Chinese Medicine Institute, Xinjiang Medical University；2Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should be addressed to [email protected]
Objective: To investigate the effects of the extract from Sophora on anti-proliferative of human colon cancer cell line HCT116, to further improve the treatment of colorectal cancer in TCM provide experimental evidence for the efficacy. Methods: Using tetrazolium blue (MTT) method, add in the final concentration of 0.6 mg/mL, 0.9 mg/mL, 1.2 mg/mL, 1.5 mg/mL, 1.8 mg/mL, 2.1mg/mL the extract from Sophora and blank medium, Calculate the cell growth inhibition rate and IC50, Through Flow cytometry Sophora extract on human colon cancer HCT116 cell growth inhibition and anti-proliferative effect. Western Blot to find EphA1 protein expression detection. The treatment group were treated with the Concentration of IC50, cells were collected at 48 and 72h,respectively.After antibody incubated in the imaging system to detect protein bands,Gray-scale analysis of protein bands using the Quantity one software,And statistical analysis. Results: Experiments shows, after treatment with different concentrations, sophora extract reversed its effect on HCT116 significantly (P<0.05), the IC50 is 1.2mg/ml and have a good dose-effect relationship, FCM detection showed sophora extract could induce apoptosis of HCT116 cell. The apoptosis rate of HCT116 cell was significantly augmented while the concentration of sophora extract increased. Protein first increased with the prolongation of the extract from Sophora,then the trend of the expression reduced. There was a significant difference between them. Conclusion: Chinese medicine Sophora extract can efficiently inhibit the proliferation and induce apoptosis of HCT116 cell and have acertain extent to EphA1 protein expression.
A-024The Clinical Observation of Tianxiangdan Capsule in Treating Angina Pectoris in
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Coronary Heart Disease (Syndrome of Qi Deficiency and Blood Stasis)
Song Xiaopeng 1, An Dongqing 1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: To observe the efficacy of Tianxiangdan Capsule in treating coronary heart disease (CHD) angina and on serum lipids. Method: One hundred and six patients with stable angina pectoris type in CHD with the syndrome of Qi deficiency and blood stasis were selected and divided randomly into three groups: Treatment group (n=37) treated with Tianxiangdan Capsule;Control group I (n=36) treated with Tianxiangdan Particles;Control group II (n=33) treated with Tongxinluo Capsule;Four weeks as one course. The clinical efficacy of the three groups were observed and their effects on blood lipids. Results: (1) The significant efficiency about the improvement of angina: in treatment group was 83.79%,Control group I was 86.11%, Control group II was81.82 %. The efficacy of the three groups were no significant difference (P>0.05).(2) The significant efficiency about the improvement of Traditional Chinese Medicine Syndrome: in Treatment group was 86. 48%, Control group I was 88.89%, Control group II was84.85%. The three group efficacy had no significant difference (P>0.05).(3) The efficient about the improvement of ECG: in Treatment group was 70.27%, Control group I was 72.22%, Control group II was69. 70%. The three group efficacy had no significant difference (P> 0.05) . (4) Stopping or reducing rate of the Nitroglycerin tablets: in Treatment group was 78. 37%, Control group I was 75. 00%, Control group II was75. 76%.The difference was not statistically significant (P>0.05).(5) Lipids before and after treatment compared to the situation: Three lipid check, Before and after treatment, there was statistically significant difference (P<0.05 or P<0.01) . Treatment group of the TG improvement was better than the Control group II (p<0.05). (6) Coagulation before and after treatment compared: Three coagulation check, Before and after treatment, there was statistically significant difference (P<0.05 or P<0.01). After treatment between the three groups, The difference was not statistically significant (P>0.05). (7) Security analysis: The three groups of patients during treatment were not found adverse effects, safety index examination also found no obvious abnormalities. Conclusion: Treatment of angina pectoris with syndrome of Qi deficiency and blood stasis by Tianxiangdan capsule was safe and effective.
A-025The Experience of Using Dihuang Pills to Treat the Chronic Cough and Asthma Treatment of Lung
Shi Suofang11First Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029, China
Pulmonary chronic cough with dyspnea , often touching refractory, has a final effect on kidny；kidney deficiency can always occur ultimately, thus has more opportunities to apply modified rehmanniae decocction. Based on clinical practice， the author elaborated the application about the syndromes of pulmonary chronic cough with dyspnea with the use of modified rehmanniae decoction, and introduced modified application ways of using it from following descriptions：Qiwei Douqi Wan， which can reinforce kidney to accept Qi and astringe the lung to relieve asthma；modified Liuwei Dihuang Wan
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and Baihe Gujin Tang，which can replenish the kidney to nourish yin and moisten lung for arresting cough；modified Liuwei Dihuang Wan and Jinshui Liujun Jian，which can tonify kidney to relieve phlegm and relieve cough with dyspnea；modified Zhibo Dihuang Wan and Guizhi Jia Longgu Muli Tang，which can nourish kidney to fall fire and regulate Yin and Yang；modified Jinkui Shenqi Wan and Zhenwu Tang，which can warm Yang to transform Qi and reinforce kidney to promote diuresis。It is thought modified rehmanniae decoction can treat the pulmonary chronic intricate and refractory diseases, having higher value of enlightenment in clinic.
A-026The influence of p38 MAPK pathway to asthmatic guinea pigs which made by Qu Fengxuanbi
Shi Suofang 1
1First Affiliated Hospital of Nanjing University of Traditional Chinese Medicine, Nanjing, Jiangsu, 210029, China
Objective: To discuss the functional mechanism of Qu Fengxuanbi (remove wind and apoplexy) Formula (Pingxiao Heji) on bronchia asthma. Method: Make bronchia asthma animal model with guinea pig sensitized by egg albumen, treat it with Qufeng Xuanbi Formula, detect the phosphorylated p38 protein expressionin in guinea pig lung tissue homogenates using immunohistochemical and Western-blot methods；Results: the two methods showed p38 MAPK phosphorylation of protein levels in the model group had significantly increased compared with the levels in dexamethasone group, Chinese medicine in high-dose and low-dose group，and protein levels in high -dose group below the low-dose group (P<0.05); Conclusion: Qufeng Xuanbi Formula is effective in the treatment of asthma，which is closely related with the inhibition of p38 MAPK pathway expression , and its inhibitory effect is dose dependent.
A-027The Studies of Regulating Blood Lipid and Antioxidant of Awei Capsules on Hyperlipemia Rats
Cui Liang 1, Xue Jie 1,2*1Traditional Chinese Medicine Institute, Xinjiang Medical University, 2Research Department ，Xinjiang Medical University，Urumqi 830011, Xinjiang, China;* Correspondence should be addressed to [email protected]
Objective: To observe the effect of Awei capsules on regulating the blood lipid, prevention of fatty liver and improving the function of liver, improving the antioxidant capacity on experimental hyperlipidemia rats. Methods: Thirty SD rats were randomly divided into five groups: control group, model group and Awei capsules high-, medium- and low-dose group. Except the control group, other groups were fed with fat-rich forage. Hgih-, medium- and Low- dose groups were given aqueous solution of the sodium carboxymethyl cellulose of Awei capsules contents. It were 1.28, 0.64, 0.32g/kg respectively. The rats of control group and model group were taken aqueous solution of the sodium carboxymethyl cellulose. It were seven weeks continuously, six days a week. Record the water intake, food intake of rats daily, and weigh weight of rats weekly. Rats were not given any food, but water for 24 hours before taken the blood. Rats were injected 10% chloral hydrate to anesthesia. Taken blood from the abdominal aorta of
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rats till to euthanasia. Separate serum. Taken the part of liver tissue into 10% neutral Formalin solution for microscopic detection, and took part of liver saved into the liquid nitrogen for completing the study of HMG-CoA RNA expression levels by electrophoresis and fluorescence quantitative PCR. The indicators of detecting: water intake, food intake, rats weight, liver weight, brain weight, heart weight, organ coefficient; liver appearance and character of the pathologic morphology of liver; TC, TG, HDL-C, LDL-C, AST, ALT, GLU, NO, MDA, activity of SOD in the serum; and the expression level of HMG-CoA in rat’s liver. Results: Compared with the blank group, the model group of male and female rats liver color was yellower; it could be observed lipid droplets and inflammatory cell infiltration phenomenon on liver cells by optical microscope detection; the difference of liver coefficient, TC, LDL-C, ALT, MDA, NO, SOD activity and AST female TG was statistically significant(P<0.05,P<0.01). Liver coefficient,TC, TG, LDL-C, AST, ALT, MDA and NO of model group were higher, SOD activity was lower. Compared with the model group, the intracellular lipid droplets and inflammatory cell infiltration phenomenon of liver were lower in Awei capsules three dose groups by the optical microscopy; liver coefficient of three dose groups on female rats, TC of female rats in three dose groups, TG of male and female rats in high dose group, LDL-C of male and female rats in three dose groups, AST of female rats in three dose groups, ALT of female rats in high dose group, ALT of male rats in high dose group, MDA in three dose groups, NO in high dose group, SOD in high- and middle- dose groups, the differences of them had significant difference ( P < 0.05, P < 0.01). The differences of other index, had no statistical significance. The other indexs of Awei capsules groups have the trend to chosing to the control group. Conclusion: Blood lipids and liver morphology, microscopic structure, function in hyperlipemia rats were abnormal in model group, so the hyperlipidemia model of rats was successful. Awei capsules can regulate blood lipid, increase the antioxidant capacity, prevent fatty liver and improve liver function. But in this study, it was not showed that the intensity of effect had correlation trend with the dose of Awei capsules.
A-028The study of quality standards and determination of validity period of Awei capsules
Hou Mingqi 1, Xue Jie 1, 2*1Traditional Chinese Medicine Institute, Xinjiang Medical University, 2Research Department ，Xinjiang Medical University，Urumqi 830011, Xinjiang, China;* Correspondence should be addressed to [email protected]
Objective: In order to use the drug safely and effectively, we establish the quality standards and predict the validity period of A Wei Capsules. Methods:①Rhizoma Bistortae 、 Cortex Magnoliae Officinalis、Rhizoma Imperatae were identified by Thin Layer Chromatograph；② The contents of Gallic Acid 、Magnolol and Honokiol were determined by High Performance Liquid Chromatograph. ③Put the sample in different humidity、examine the level of absorbing water and get critical relative humidity. ④Classical constant temperature test: put capsules at different temperature of 80℃、90℃、100℃ ,examine sample at different time, the change of different indexes content at different time was determined to estimate the validity period of the capsules. ⑤According the items of China Pharmacopoeia ,we determine the content of water、 loaded quantity differences 、crumbling time limit and microbial limits. Results:①Rhizoma Bistortae、Cortex Magnoliae Officinalis、Rhizoma Imperatae have same colour spots on the same location according the reference substance and the negative samples have no interference. ②Determined by High Performance Liquid Chromatograph,the content of GallicAcid was 1.081±0.064mg/g, The contents of Magnolol and Honokiol were respectively
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14.56±0.058mg/g、6.15±0.036mg/g. ③ The critical relative humidity was 72%,It presented that we should control surroundings humidity under72% when producing and storaging drug . ④The activate energies of different indexes was calculated based on Arrhinius formula and the validity period was predicted 2.75 years for Magnolol, 1.77 years for Honokiol. ⑤ We should control the content of water was under 7%, the methods of crumbling time limit were adding 700ml water at 37℃,loaded quantity differences and microbial limits were done by the methods of China Pharmacopoeia. Conclusion: Through multicomponent andmulti-index, we establish the standard of A Wei Capsules. We can predict the validity period of capsules with the index of contents. All of these can provide the experiment evidence of evaluating drug quality and storage times.
A-029The Study on Medicinal Value and Reduce the Toxicity with Processed of Aconitum soongaricum Stapf in Xinjiang
Wu Chao1, Jiang Lin1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: To research the period of time processed in Aconitum soongaricum Stapf in Xinjiang alkaloid content and the change of the toxicity and pharmacological, determine the Aconitum soongaricum Stapf in Xinjiang processed in method. Methods: 1.Used by reveed-phase HPLC before and after processed in Aconitum soongaricum Stapf in Xinjiang alkaloid content changes. 2. By using mice following intraperitoneal injection method for determination LD50 of different processed products of Aconitum soongaricum Stapf. 3. Used radiant heat stimulation and acetic acid induced writhing test and investigated analgesic function of different processed products of Aconitum soongaricum Stapf. 4. Observed the different processed products of Aconitum soongaricum Stapf to AA anti-inflammatory effect of rats. 5. To observe the different processed products of Aconitum soongaricum Stapf to CIA anti-inflammatory effect of rats. Results: 1. Aconitum soongaricum Stapf in Xinjiang was the aconitine content was higher , the content of Mesaconitine and Hypaconitine was low, processed products of double ester type of alkaloid content was reduced, single ester type alkaloid content increased. 2. The toxicity order of different processed products of Aconitum soongaricum Stapf: Product > Literature method > Pharmacopoeia method (2010) >Kazakh method; The result showed that the LD50 were 3.079 2 g/kg, 26.219 1 g/kg, 10.587 3 g/kg and 39.870 2 g/kg respectively. 3. (1) Compared with contrast group, The administration groups are able to significantly extend mice from radiation caused by thermal stimulation of mouse tail-flick latency except the low dose from Pharmacopoeia method (2010) and Kazakh method; (2) Compared with contrast group, The administration groups could reduced rat torsion times (P<0.01or P<0.05), which had analgesic effects except the low dose from Kazakh method. 4. (1) The swelling degree and arthritis index of Aconitum soongaricum Stapf and all of its processed products groups were lower (P<0.01or P<0.05). (2)The content of Cort and IL -1β in serum of positive group，Aconitum soongaricum Stapf and all of its processed products groups were lower (P<0.01or P<0.05). 5. (1) The swelling degree of Aconitum soongaricum and all of its processed products groups were reduced. (2)By light microscopy observation, the treatment group synovial histopathological form had improved significantly. (3)Compared with model group, give medicine 6 weeks, the content of SA,IL-2 and TNF-α with The administration groups had significant differences(P<0.01or P<0.05) except
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its Kazak method processed product group. Conclusion: (1)Aconitum soongaricum Stapf processing synergia from action mechanism and the diester-type alkaloids content reduced , single ester type alkaloid content increased related.(2) Acute toxicity and pharmacodynamics experiment shows that literature method processed products and pharmacopoeia method (2010) processed products of analgesia and anti-inflammatory effects are much better than Kazakh method processed products, But pharmacopoeia method(2010) processed products of toxic smaller than literature processed products.(3)Aconitum soongaricum Stapf after processing can reduce the toxicity and increase effect, has some of the medical values, The pharmacopoeia method(2010) processed products of acute toxicity, pharmacodynamics are better than the other two kinds of processing methods, So optimization pharmacopoeia method(2010) as Aconitum soongaricum Stapf reduce the toxicity and increase effect of the processing technology.
A-030The study on sustained-release technology of Tianxiangdan capsules
Wang Jia1, Jiang Lin1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: To study the preparation technique of Tianxiangdan sustanined-release capsules, and evaluate its release characteristics in vivo and vitro. Methods: 1) On the foundation of extraction technology in Tianxiangdan capsules, instant-release granules were prepared by wet granulation. First, using disintegration time as a evaluation parameter, we inspected single accessory of the capsule, then through the method of orthogonal test design get the best mixed proportion of excipients. 2) Selected inclusion method of volatile oil to define the best inclusion method, the ratio and yield of the oil in inclusion complex was used to evaluate the technology based on the orthogonal design, in which the proportion between β-cyclodextrin and volatile oil, the proportion between β-cyclodextrin and water and rubbing time were considered to be 3 main factors. 3) The sustained release granules were prepared by solid dispersion technique, to optimized select the best mixed proportion of sustained release excipients by uniform design experiment. Investigated every released conditions and established standard of release degree in vitro, selected the best mixed proportion of instant-release granules and sustained release granules. 4) The cumulative release percentage in vitro of sustanined-release capsules was determined by rotating basket method, and discussed released mechanism of the drug. 5) According to determination of hemodynamic parameters in anesthetized rats and the experiment that is affected of rat models on myocardial ischemia by ISO, preliminary explored the pharmacokinetics of sustained-release preparation in body. Results: 1) The best mixed proportion of instant-release excipients that MCC : CMS-Na : L-HPC is 1:2:3. 2) The optimal inclusion procedure is as follows：the proportion of volatile oil and β-cyclodextrin was 1:6 , the proportion of water and β-cyclodextrin is 5:1 and rubbing for 10 min. 3) The best mixed proportion is EC: HPMC: PVP = 17:2:1 in sustained release granules and finally making 60% instant-release granules and 40% sustained release granules are mixed in capsules, the drug release pattern is conformed to the first order kinetics[r1=0.9927 、 r2=0.9511]. 4) Tianxiangdan sustained-release preparation group compared with the ordinary preparation group that LVSP and + dp / dt had statistical differences (△P <0.05), it is show that the improvement effect of hemodynamics parameters in the sustained-release preparation group is better than the ordinary ,which
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are myocardial ischemiahe rats. Conclusions: 1) The preparation method for Tianxiangdan sustanined-release capsules reported in this paper is reasonable and feasible. 2) The prepared sustained-release capsules had a better sustained-release effect,the drug was released stability, and achieved the goal of sustained-release in the drugs.
A-031Theory of Acupuncture "reinforcing" and "reducing"
Cai Hua1, Yin Gaizhen2*1Lanzhou Military Region, Urumqi General Hospital Department of Rehabilitation Medicine, Urumqi, 830000, China; 2Xinjiang
Medical University School of Medicine Department of Acupuncture basis, 830054, Urumqi, China;* Correspondence should be
addressed to [email protected]
Acupuncture reinforcing-reducing means that acupuncture treatment can directly play an important role in the traditional acupuncture attention. Acupuncture reinforcing-reducing can directly original from the theory of 《Huangdi Neijing》, long-term guiding clinically therapeutic and stimulate quantity. For the later a variety of physical stimulation therapy to lay the foundation. This article from the theory of acupuncture reinforcing-reducing can direct the origin of the concept, this paper discusses the basis of reinforcing and reducing. 1, syndrome differentiation and accurate. 2, to grasp the meridian line followed Qi and blood circulation. 3, relate about “bring about the desired sensation”. 4, illustrate relying on acupuncture point and so on. At the same time, given an example as acupuncture needle, moxibustion, cupping, scraping, etc in clinical application can direct the research.
A-032Assessment of using the Tianxiangdan Capsule to Help Rehabilitation Therapy in Coronary Artery Bypass Grafting
Li Jingru1, Wang Xiaofeng1*1 Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: To evaluate the clinical efficiency of using the Tian Xiang Dan Capsule to help rehabilitation therapy in Coronary Artery Bypass Grafting (CABG). Method: 77 Patients of CABG randomly were divided into treatment group (n=37) and control group (n=40). Control group were given regular treatment, while treatment group were added the Tian Xiang Dan Jiao Nang with regular treatment, one month was a period of treatment and three periods of treatments were observed the effect. Results: After a 3-month treatment. Total effective rate in the treatment group was 78.38%.In the control group was 55.00% (P <0.05); evidently effective rate in the control group was 2.5%, in the treatment group were 10.81%, two groups were different significantly (P <0.05). Scoring of symptoms was decreased. ( P<0.05), The scores in terms of physical functioning、 role-physical、 bodily pain、 general health，vitality、social functioning、 reported health transition of patients in the experimental group according to SF-36 scale were significantly higher than those in the control group (P<0.05 ). The scores in terms of physical limitation、 anginal stability、 anginal frequency、 treatment satisfaction and Disease perception of patients in the experimental group according to SAQ scale were significantly
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higher than those in the control group (P<0.05 ). In terms of 6 min walking test was evidently effective rate in the treatment group than that in the control group (P<0.05). The function of heart LVEF before and after the test, two groups were effective (P<0.05), but there were not statistical different between two groups (P>0.05). Conclusions: (1) the Tian Xiang Dan Jiao Nang can improve the relation symptom. (2) Tian Xiang Dan Jiao Nang can improve function of LEVF、 the quality of life and the actual living ability of CABG patients.
A-033Clinical study on Differential Treatment of Traditional Chinese Medicine in the treatment of 210 Cases of Sub-health State in Urumqi
Liu Tao1, Hu Xiaoling1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: To observe the Chinese medical type of syndrome characteristic and the clinical efficacy of differential treatment of TCM in treatment of 210 cases of sub-health state, we will get some references from the study. Methods: Using Task Force developed the questionnaire to screen the sub-health state out of people, according to "Clinical Guidelines of Chinese Medicine on sub-health" dialectic, and to identify syndromes, the study of evidence-based rules. Using different treatment of traditional Chinese Medicine, observe the people for intervention treatment. The observation and assessment of its clinical efficacy were observed by Symptom Rating Scale. Results: (1)210 Cases of Sub-health State with syndrome of stagnation of liver qi the distribution of the majority (38.57%), followed by syndrome of qi deficiency (31.43%), syndrome of yin deficiency of liver and kidney (20.48%), syndrome of phlegm-heat attacking internally(9.52%); the card-type distribution in terms of gender difference was statistically significant (P<0.05); various syndromes in all age groups the difference was statistically significant(P<0.05).(2) there were obvious statistical differences between the collected scores of the19 main body symptom evaluated before the treatment and that after treatment(P<0.05). Conclusion: (1)210 cases of sub-health state in Urumqi city, syndrome of stagnation of liver qi in order to permit the most prevalent, many syndrome of stagnation of liver qi 、syndrome of qi deficiency and syndrome of yin deficiency of liver and kidney people are female, many syndrome of phlegm-heat attacking internally people are male; Many syndrome of stagnation of liver qi people in 18～ 39 age, syndrome of qi deficiency people are older than 40 age. (2)In treatment of sub-health state differential treatment of traditional Chinese Medicine effectively improves clinical syndrome.
A-034Coronary Heart Disease merge 2-hour postprandial glucose patient of Traditional Syndrome Differentiation and ZeXieDanMingYin intervention research
Zeng Lin1, An Dongqing1*, Li Kaili11Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: Explore cardiovascular hospitalized patients with abnormal condition of sugar metabolism,
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Coronary Heart Disease merger 2-hour postprandial glucose patient of Traditional Syndrome rule, observe CEC in Coronary Heart Diseas with diabetes mellitus and coronary merger 2-hour postprandial glucose abnormalities in patients with level changes and discrepancy and oral ZeXieDanMingYin and rational lifestyle intervention six weeks after observe its blood glucose, lipid profile and circulatory endothelial cell parameters such changes.Methods:Select January 2010 to September 2010 period , in Xinjiang Medical University affiliated hospital of traditional hospitalized patients diagnosed Coronary Heart Disease、Coronary Heart Disease with diabetes and Coronary Heart Disease merge 2-hour postprandial glucose patients, observe CEC quantity change, Coronary Heart Disease merge 2-hour postprandial glucose patients, according to Traditional Syndrome 6 dialectical divided into XunXueYuZu, QiZhiXinXiong, TanZhuoBiZu, QiYinLiangXu, XinShenYinXu, XinShenYangXu, analyzing its characteristics. The control group were randomly divided into two groups, with western medicine foundation treatment, including regular enlarge crowns, lipid, step-down treatment such as, but not taking oral, The treatment group based in western medicine treatment combined with alisma orientalis Dan Ming drinks and rational lifestyle intervention six weeks observation blood sugar, blood fat and circulatory endothelial cell parameters such changes. Results：1、2-hour postprandial glucose rate in Coronary inpatients 62% 2、 Traditional syndrome differentiations with TanZhuoBiZu type at most, followed by most XinXueYuZu type, 3、When 2-hour postprandial glucose the circulatory endothelial cell significantly higher than CHD group.4、Add ZeXieDanMingYin and rational lifestyle intervention six weeks after can significantly reduce TC 、TG、LDL – C、 PBG (P < 0.05)，HDL-C、LDL-C without opparent change (P > 0.05), a clinical medicine effect is better (P < 0.05).Conclusion: China coronary inpatients in the vast majority of merger sugar metabolic abnormalities, and need through OGTT experiments accurately find these merger with abnormal glucose metabolism, always detection FPG diagnosis most sugar metabolic abnormalities. Through the Traditional Syndrome differentiation coronary heart disease merger 2-hour postprandial glucose in the process, we found that the most common TanZhuoBiZu, so we think in clinical medicine treatment on the basis of add with HuaTanTongLuo drug of traditional Chinese medicine to prevent coronary heart disease merger 2-hour postprandial glucose, reduce the morbidity and mortality in patients with, raise its quality of life to have the extremely vital significance.
A-035Experimental Study in Effects of Xu-zao-geng-ping Powder on the Histomorphology Change of Arteries and MMP-9, TIMP-1 in Climacteric Hypertension Rats
Li Hongxia1, Lin Xue1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: Through study of the effects of Xu-zao-geng-ping Powder in climacteric hypertension rats on histomorphology change of vascular and MMP-9， TIMP-1 research to explore reverses vascular remodeling and possible mechanism in Climacteric hypertension rats. Methods: Surgical remove ovaries of SHR rats to establish climacteric hypertension model and evaluate it. SHR rats were randomly divided into climacteric hypertension traditional Chinese medicine, climacteric hypertension western medicine, climacteric hypertension, fake operation and SHR groups. Set Wistar group as a normal control group. Drench Xu-zao-geng-ping powder to climacteric hypertension traditional Chinese
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medicine group and Valsartan capsule to climacteric hypertension western medicine. The other groups were drenched saline solution. Observation of morphological changes in vascular tissues of rats in each groups, measuring MT, LD, MT/LD, monitoring changes of blood pressure in groups of rats and comparing MMP-9, TIMP-1 level in serum of different groups. Results: 1. Vaginal smear of model rats showed no renew of estrous cycle change. Rat serum E2, T, P levels were significantly reduced(P<0.05); FSH and LH level were significantly increased (P<0.05). 2. Compared with the climacteric hypertension group, fake operation group and SHR group, blood pressure of climacteric hypertension traditional Chinese medicine and climacteric hypertension western medicine group in rats dropped significantly before and after drenching drug. 3. Morphological changes was significant in vascular tissues of climacteric hypertension rats. Their LD reduce than other groups, MT, MT/LD significantly increased (P<0.05). LD of fake operation, SHR group decreased than climacteric hypertension traditional Chinese medicine, climacteric hypertension western medicine and Wistar group, but MT, MT/LD of fake operation increased (P<0.05). LD of climacteric hypertension traditional Chinese medicine and climacteric hypertension western medicine groups decreased than Wistar group, but their MT, MT/LD increased (P<0.05). 4. The serum levels of MMP-9, TIMP-1 of climacteric hypertension group significantly increased than the other groups (P<0.05). Compared with the climacteric hypertension, fake operation and SHR groups, MMP-9 levels in serum of climacteric hypertension traditional Chinese medicine and climacteric hypertension western medicine groups decreased, but TIMP-1 levels increased (P<0.05). Conclusion: 1. Established climacteric hypertension rat model by removal of ovaries in SHR rats. 2. Vascular tissue pathological changes can be increased in climacteric hypertension rats. 3. MMP-9 levels of serum increased to stimulate TIMP-1 level of serum compensatory increased. Disorder of MMP-9 and TIMP-1 broke the dynamic balance result the vascular remodeling. 4. Findings of this study indicate that the Xu-zao-geng-ping Powder can reduce blood pressure in climacteric hypertension rats significantly and reduce vascular tissue damage caused by reducing MMP-9 and increasing TIMP-1 in serum to restore ECM control balance. It probably is the effective mechanism for preventing and reversing vascular remodeling in climacteric hypertension rats.
A-036Experimental study on Inflammatory factors in serum of rats with Rheumatoid Arthritis treated by PengCao Removing Bi-syndrome Decoction
Tian Xinwei1, Liu Chunjing1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China
Objective: To observe the mechanism of PengCao Removing Bi-syndrome Decoction(PCRBD) on Rheumatoid Arthritis(RA) and the level of Inflammatory factors IL-1β、TNF-α and PGE2 in serum of rats with Rheumatoid Arthritis treated by the decoction. Methods: Randomed out 10 rats as blank group， the remaining 50 rats for replication collagen inductive arthritis (CIA) model，divided them into Chinese high，medium， low group， tripterygium glycosides(TG) group and model group，keeped the TG group as the positive control group, observed 6 weeks，evaluated the inhibition of PengCao Removing Bi-syndrome Decoction on the joint swelling of the double back foot ，the joint pain index of the double back foot， the weight gaining and the IL-1β、 TNF-α and PGE2 in serum. Result: Compared with model group， the joint swelling of the double back foot(P<0.01，P<0.05)，and the joint pain index of the double back foot (P<0.01)of the rats in the treatment groups are significantly
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restrained；and the rate of the weight gaining improved significantly treated by the Chinese medium and high dose group (P<0.01，P<0.05)； the level of IL-1β、TNF-α and PGE2 in serum of rats are much better than the model group(P<0.01)；the inhibitory effect of Chinese medium dose group on the level of IL-1β、TNF-α is better than the TG group(P<0.05),the high dose group reduced the level of PGE2 better than the others. Conclusion: The usage of PCRBD could restrain the joint swelling of the right back foot and the joint pain index of the double back foot of the rats significantly. The mechanism of relieving the symptoms of rats with RA maybe related to reducing the level of proinflammatory cytokines IL-1β， TNF-α and PGE2 in serum，and the decoction is a pure Chinese herbal compound worthy of further research and development.
A-037A Study on Influence of Xu Zao Geng Ping granule on Blood Pressure Variability of the Rats with Menopause Hypertension
Gan Qinglei1, Lin Xue1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: Through study of the effects of Xu-zao-geng-ping-powder in menopause hypertension rats on histomorphology change of BP, BPV and CA, NPY, NT, E2, FSH, LH research to explore reverses reduce the BP and BPV possible mechanism in menopause hypertension rats. Methods: Established menopause hypertension rats model by operating, model evaluate by the vaginal smear and serum sex hormone levels in rats and randomly divided them into menopause hypertension TCM medicines, menopause hypertension western medicine, menopause hypertension control group, fake surgery group, SHR group and Wistaris a normal control group. Respectively to intragastric administration xu-zao-geng-ping-granule to menopause hypertension TCM medicines group, valsartan capsule to menopause hypertension western medicine group and saline solution to the others groups. Instrument for non-invasive measurement of blood pressure(BP) in animals, at weekly measurements twice all of the arterial blood pressure of rats to compare different groups in arterial BP for observing the changes in BP of rats. Comparative observation changes of BP, BPV and CA, NPY, NT, E2, FSH, LH in different groups. Results: 1.By results of the vaginal smear and serum sex hormone levels in menopause hypertension model for evaluating success: compared with the fake surgery group and SHR group, model rats after operation, menopause hypertension rats serum E2, T, P levels significantly reduced(P<0.05); menopause hypertension rats serum FSH, LH levels significantly increased(P<0.05). 2.BP: TCM medicines group and western medicine group’s BP is significantly declined(P<0.05). 3.BPV: compared with the menopause hypertension control group, TCM medicines group and western medicine group’s BPV is significantly declined(P<0.05). 4. Neurotransmitter indices: compared with the menopause hypertension control group, TCM medicines group and western medicine group’s BPV is significantly increased(P<0.05). 5. Endocrine indices: compared with the menopause hypertension control group, TCM medicines group’s E2 is declined(P<0.05); TCM medicines group’s FSH, LH is increased(P<0.05). Conclusion: 1. Through the removal of essential hypertension in rat ovaries, menopause hypertension rats model can be successfully established. 2. The xu-zao-geng-ping-granule can reduce the climacteric hypertension rats’ BP and BPV. 3. The xu-zao-geng-ping-granule can regulate the neurotransmitter and endocrine indices of the climacteric hypertension rats which improve
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the menopause hypertension rats’ internal environment.
A-038The characteristics of the syndrome TCM distribution of femoral head necrosis in Urumqi region
Jin Wuyong1, Meng Qingcai1*1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;
*Correspondence should be addressed to [email protected]
Objective: To explore the characteristics of the syndrome TCM distribution of femoral head necrosis in Urumqi region and offer a reliable basis on therapy for femoral head necrosis in principles, methods, prescriptions, drugs. Method: We collected 358 patients out of and in hospitalization who meet the diagnosis standard of femoral head necrosis from our co-operation hospitals in Urumqi city randomly and collected their information using the survey questionnaire of femoral head necrosis. The data were
analyzed by univariate frequency distribution, 2x test. Results: (1) blood stasis type, liver and kidney
deficiency type frequency in 28% to 64%, and Blockage of the meridians type and blood stasis blocking collaterals type and frequency of each of 4%, blood stasis and kidney deficiency Between the imaginary frequency of symptoms very significant difference (p<0.05); (2)Blockage of the meridians type and frequency of phlegm and blood stasis blocking collaterals did not differ between; different age and gender was significant difference between (p<0.05); (3) Different TCM syndrome distribution and gender, age comparison, there was a significant difference was significant (p<0.05); (4) Comparison of different syndromes, in addition to ethnic difference was not statistically significant, the remaining duration of disease, incentive distribution, occupation, respectively, the difference was significant (p<0.05). Conclusion: The TCM syndrome distribution of femoral head necrosis in Urumqi region is ：The general syndrome is qizhixueyu syndrome，the next is ganshenkuixu syndrome，jingluobizu and tanshizuluo syndrome are rare.
A-039The Correlation of Uygur and Han Nationality of TCM Syndrome Type of Premature Coronary Heart Disease with Blood Lipids and Inflammatory Factor
Zhai Xueqin1, Wang Xiaofeng1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: To investigate the correlation of Uygur and Han nationality of TCM syndrome type of premature coronary heart disease with blood lipids and inflam-matory factor. Methods: Recruited were 152 Uygur and 156 han patients with PC-HD first confirmed by coronary angiography at the Heart Center of First Affiliate-d Hospital of Xinjiang Medical University and Traditional Chinese Medicine of Xinjiang Medical University. Another 160 volunteers were recruited internal medicine. T-CM syndromes were typed physicians or those having higher professional titles at the cadiac internal medicine. The contents of triglyceride (TG) and total cholester-ol (TC)were detected using oxidase method, the high-
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density lipoprotein cholesterol(HDL-C) level was detected using chemical modification encymic method, the low density lipoprotein cholesterol(LDL-C)level was detected using selective dissolution. The fibrinogen (FiB) level was detected using Clauss congeal principle. The high-sensititive reactive protein (hs-CRP) level was detected using Scattering turbidity method. The interleukin-6 level was detected by ELISA. Results: (1) The distribution of TCM syndrome of Uighur PCHD as follows: Huizhuotanzu type(32.24%)> Qixux-ueyu type (31.58%) >hanningxinmai type (18.42%) >qiyinliangxu type (10.53%)>yan-gqixushuai type (7.24%); The han distribution as follows:Qixuexueyu type(35.90%)>Huizhuotanzu type(25.00%)>Han-ningxinmai type(22.44%)>Qiyinliangxu type(12.18%)>yangqixushuai type (4.49%). (2)two nationals of syndrome types exist fat metaboli-sm disorder obviously with PCHD patients, showing a significantly higher level of TG, HDL-C level of reduced significantly, and LDL-C level rises, the statistics w-as significantly difference (P<0.01, P<0.05); And han nationality with card model more, Huizhuotanzu type of TG was higher significantly, HDL-C was fall, the stati-stical was difference significantly than han (P<0.01, P<0.05), Hanningxinmai type of TG was higher significantly too, Qixuxueyu and Qiyinliangxu was higher of T-G、LDL-C significantly (P<0.01, P<0.05). (3) Two nationals of syndrome types exi-st inflammation obviously with PCHD patients, the content of Fib、 hs-CRP and IL-6 were rise. The content of FiB、hs-CRP and IL-6 were increased in Uighur of P-CHD patients (P<0.01), Hanningxinmai of IL-6 and hs-CRP were rising (P<0.01), Qixuxueyu of IL-6 was rising too; The content of hs-CRP 、 FiB 、 and IL-6 were increased in Han of PCHD patients of Huizhuotanzu and Hanningxinmai(P<0.01), Qi-xuxueyu of IL-6 and FiB was rising too(P<0.01). Two national of Hanningxinmai and Qixuxueyu were difference of FiB (P<0.05). (4)To analysis the correlation of TG、TC、HDL-C、LDL-C、FIB、hs-CRP and IL-6 of TCM syndrome type by spe-arman rank. Uygur of PCHD patients of Huizhuotanzu of TG、FiB、 IL-6 are rela-ted positively， related with HDL-C negatively, relevant coefficients: r=0.469, r=0.464, r=0.999，r=-0.382. Qixuxueyu was related positively with FiB, correlation coefficient：r = 0.720, the differences are statistically significant (P<0.01, P<0.05). Ha-n of PCHD patients of Huizhuotanzu of TG、FiB、 IL-6 are related positively two， related with HDL-C negatively，relevant coefficients: r=0.445, r=0.372, r=0.999，r=-0.370. Qixuexuyu was related with TG and FiB positively，relevant coefficients we-re r=0.471, r=0.459, the differences are statistically significant (P<0.01, P<0.05). Conclusion: The correlation of Uighur and Han nationality of TCM syndrome type of premature coronary heart disease with blood lipids and inflammatory factor. Uygur, which can provide the basis and the guide for the research for PCHD TCM syndrome type.
A-040The Correlation of Uygur and Han Nationality of TCM Syndrome Type of Premature Coronary Heart Disease with Molecular Markers of Prethrombotic State
Liu Hao1, Wang Xiaofeng1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: To investigate the correlation of Uygur and Han nationality of TCM syndrome type of premature coronary heart disease with the markers of prothrombotic state. Method: 626 eases underwent CAG in Xinjiang Medical University, and were divided into PCAD 340 patients(154 cases Han Uygur,186 cases of late onset CHD 286 patients(Uygur)in 106 cases,180 cases of Han)in two
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groups, patients after hospital admission records for patients with traditional risk factors, by hospital heart internal medicine attending physicians and above the syndrome differentiation of Traditional Chinese Medicine typing, all the patients in the second days after admission to the morning fasting venous blood 2ml mining, separation of serum. Fibrinogen(F g/FIB) using automated latex enhanced immunoturbidimetric method, tissue plasminogen activator(T-PA), plasminogen activator inhibitor(PAI-1),using ELISA method for the determination of-two; D dimer(DD), P-selectin(P-Sel)using the ELISA double antibody sandwich assay test check. Results: (1)The Uygur PCAD patients of age, positive family history and Han Nationality early onset group with a significant difference, age, smoking, drinking,overweight and Uighur late-onset group CHD had statistical significance(P<0.01), Han PCAD patients in age, smoking, drinking, dyslipidemia, hypertension, positive family history and Han late-onset group CHD had statistical significance(P< 0.05,P <0.01).(2)the Uygur PCAD Qi Deficiency and blood stasis, turbid sputum an-d other syndromes and Han PCAD were statistically significant(P<0.01),PCAD abusive cloud Uygur phlegm and other syndromes and Uygur late onset group with ca-rd type comparison there is statistically significant(P<0.01).(3)To compared Uygur And Han Nationality Of PCAD patients of PAI-1 Fg and DD with the same Nationality groups are statistically significant， the PAI-1 of turbid sputum syndrome, P-Sel, Fg, DD of Qi yin phlegm blocking with the same Nationality syndrome groups are statistically significant; Uygur patients with PCAD turbid sputum syndrome and P-Sel, T-PA,PAI-1,Fg and positively related, and DD is negative correlation, turbid sputum syndrome and P-Sel, PAI-1, was positively related to Fg, T-PA is negative correlation,Qi deficiency and blood stasis was positively correlated with DD. Conclusion: smoking, drinking, overweight, dyslipidemia, hypertension, diabetes, in the Xinjiang region of the CHD patients are accounted for a considerable proportion of the Uighur patients with PCAD, younger age, positive family history of Han nationality patients with PCAD significantly increased(44.1%); turbid sputum, Qi deficiency and blood stasis is the four group is the main type of Chinese medicine, and Uygur PCAD dirty chaotic phlegm was significantly higher than that of Han nationality early-onset group; Uygur and Han nationality patients with PCAD syndrome type of Traditional Chinese Medicine and the markers of Prethrombotic state there is correlation, can serve as a guide for PCAD syndrome type of traditional Chinese Medicine to provide basis for the study of the micro substance.
A-041The Experimental Study of Qihong Decoction on the effects in rat model with isoproterenol induced chronic heart failure
Wang Min1, Wang Xiaofeng1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: To study the treatment of Qihong Decoction in rat model with isoproterenol induced chronic heart failure and to observe the effects on brain natriuretic peptide (BNP) and matrix metalloproteinase -9 (MMP-9) and to explore the mechanism of its prevention and treatment of heart failure. Methods: The method of subcutaneous injection of isoproterenol copy cardiac failure model, determination of cardiac function after 6 weeks that model successful, will become the model rats were randomly divided into model group, low dose of Qihong decoction, Qi Hong granule middle dose group high dose of Qihong decoction and digoxin group, were administered again after 4 weeks, cardiac function
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measured using the enzyme immunoassay of serum BNP, MMP-9 levels, and take heart tissue that re-recording, calculating the heart weight index of treatment, morphological features of cardiac pathology. Results: ①compared with blank control group, the model group declined in cardiac function ,LVEDD、LVESD increased and EF、FS decreased . Every treatment groups significantly improved cardiac function after 4 weeks, LVEDD 、 LVESD decreased and FS 、 EF increased.②compared with blank control group，heart weight index was ignificantly increased among model group, Qi Hong low dose group and digoxin group, while Qi Hong granule medium- dose and high- dose were no significant difference (P> 0.05); Compared with model group, the treatment groups of heart weight and heart weight index decreased, there was significant statistical difference (P <0.01). ③ Compared with blank control group, the content of BNP, MMP-9 increased significantly in model group (P <0.01); compared with model group, the content of BNP, MMP-9 decreased in every treatment group (P <0.01), Qi Hong granule which high, medium dose group were obviously better than that of digoxin group (P <0.01), Qi Hong granule low-dose compared with digoxin group no significant difference (P> 0.05).Conclusion: Qihong decoction can significantly alleviate symptoms, improve heart function, regulating cardiac endocrine function in the heart and reverse ventricular remodeling.
A-042The Influence of Oubo Rhinitis Granule to Allergic Rhinitis Rats Th2 Cells and Its Factors、IgE and Mast Cells
Zhang Xinri1, Li Yanhua1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: The main objective of this essay is to study the impact of oubo rhinitis electuary for the Th2 serum cells of the anaphylactic rhinitis experimental rats and its other factors cells (IL-4, IL-5); to observe the change of the nasal mucosa mast cells of the anaphylactic rhinitis experimental rats; to discuss the foundation and therapeutic effect of the model of the anaphylactic rhinitis experimental rats. Methods: There are 60 healthy adult wistar rats, which can be divided into same numbers of males and females. These rats will be made for rat models with egg albumin sensitization. Then, the successfully models will be going to random allocation. Next, after feeding them medicine for 28 days, it can draw blood from carotid artery and separate their serum. Furthermore, the ELISA methods should be used to detect the amount of total IgE, IL-4 and IL-5. Also the flow cytometry (FCM) should be used to inspect Th2 cells contents of peripheral blood cells. Lastly, with 1% armor aniline blue staining watches the changes of the experiment rats’ nasal mucosa mast cells. Results: 1) Compared with the normal groups, the serum level of the model rats striking rise about IL-4, IL-5 and IgE (P<0.01), but the serum level of the therapeutic rats bring down about IL-4, IL-5 and IgE (P<0.05). Also, the difference is remarkable between therapeutic rats and the masculine rats (P<0.05). 2) Compared with the normal group, the Th2 cells of the model rats clear grow (P<0.05); compared with the model group, the Th2 cells of the oubo rhinitis electuary small dose group have no obvious difference (P>0.05); but the Th2 cells of the oubo rhinitis electuary medium and high dose group evident decrease (P<0.05); at the same time, the Th2 cells of XinQin group sharp down (P<0.05), the Th2 cells of the oubo rhinitis electuary medium and high dose group have no distinct change when we compared with the XinQin group(P>0.05). 3) The Pathological pictures show the Mast cell in nasal mucosa of model rats are
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increase, each give medicine rats are reduce, the average number of high dose group’s Mast cell in nasal mucosa is minimum. Conclusion: In order to explore the antiallergic effect and to alleviate the allergic rhinitis symptoms, the oubo rhinitis electuary may reduce the serum of the experimental rats of the amount of IL-4, IL-5, total IgE and Th2 content and the nasal mucous membrane of the number of mast cells.
A-43The pathological effect of PengCao Removing Bi-syndrome decoction on synovial inflamation and bone erosion of rats with Collagen-Induced Arthritis
Chen Chang1, Liu Chunjing1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;
To study the mechanism of PengCao Removing Bi-syndrome Decoction on Rheumatoid Arthritis(RA) from the pathological and immunohistochemical perspective by observing Histopathology affect on synovial tissue and the regulation mechanism of bone erosion of rats with collagen-induced arthritis(CIA) treated by the decoction. Clean level Wistar rats，60，male and female evenly, weigtht (110±20)g， Random out 10 rats as blank group， the remaining 50 rats for replication collagen inductive arthritis (CIA) model, 7 days after causing inflammation we began to observe and record systemic articular damage degree, every 7 days record 1 times， the rats with arthritis index (AI) > 5 points are made mould success. Then we divided them into Chinese high， medium， low group， tripterygium glycosides (TG) group and model group， observe 6 weeks， evaluate the arthritis index(AI) , the pathological changes of synovial tissue and the Osteoprotegerin (OPG)、Receptor Activator of NF-κB Ligand (RANKL) expressions in cartilage of rats. Compared with model group， the AI of Chinese group and Tripterygium group was significantly lower（P<0.05，P<0.01）,pathological changes in synovial tissue scores were significantly different, （ P<0.05 ， P<0.01 ） ,through pathological morphology observation， PengCao Removing Bi-syndrome Decoction of high， medium and dose group and TG group， synovial cell hyperplasia degree is lower than model group significantly， inflammatory cells infiltration and synovial vasculitis performance were significantly reduced. PengCao Removing Bi-syndrome Decoction could reduce the AI of rats， and inhibit synovial cell proliferation ， fibrous tissue hyperplasia， inflammatory cell infiltration and macrophage infiltration， which is a pure compound preparations worth of a further development and research. The RA regulatory mechanisms of Articular bone erosion may be related to inhibition of RANKL expression in articular cartilage of rat with CIA and of no obvious effect on the expression of OPG.
A-044Treatment of QHD Tongluo Experimental Study of hyperuricemia
Wang Danhui1, Yushanjiang Aikemu1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China
Objective: The observation dispels wet removing extravasted blood to pass winds Fang Fills the stomach to the big mouse blood uric acid, the kidney function, the empty stomach blood sugar, the empty stomach insulin influence, discusses dispels wet removing extravasted blood to pass winds the
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side to treat the hyperuricaemia the action mechanism. Method: Establishment hyperuricaemia big mouse model: The SD big mouse take the adenine, the ethylamine butylacohol as makes the mold medicinal preparation, establishes the hyperuricaemia animal model. Sets up the normal group， the model group，the western medicine group，the Chinese native medicine low monitoring team， the high monitoring team. Feeds 3 weeks to observe separately tests 7 day, tests 21 day, determines the big mouse's blood uric acid， the urea nitrogen, the myo- bitter wine， the empty stomach blood sugar， the empty stomach insulin; Takes the kidney left side in 21st days, observes the kidney pathology change. Results: Dispels wet removing extravasted blood to pass winds the side to have the function which the suppression model big mouse blood uric acid, the myo-bitter wine, the urea nitrogen, the empty stomach blood sugar, the empty stomach insulin advance. Conclusion: Dispels wet removing extravasted blood to pass winds the side to have reduces the hyperuricaemia model big mouse blood uric acid the function.
A-045The Influence of Xihong Kang Fang Using to the diabetes nephrosis patient phase III Hcy CysC and UAER
Feng Chengcheng1, Yushangjiang Aikemu1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China
Objective: To observe the clinical effect of “xihong kang prescription” used to diabetic nephropathy (phase III) patients with homocysteine (Hcy), Cystatin C (CysC), urinary albumin excretion rate (UAER). And to probe the clinical effect of xihong kang prescription clinical symptoms of the diabetic nephropathy (phase III) patients. Methods: 62 subjects with diabetic nephropathy phase III patients were randomly divided into the treatment group (32 cases) and the control group (30 cases). Patients of the treatment group took xihong kang prescription with modern medicine conventional therapy. Patients of the control group only took the modem medicine only. Two months after the treatment, compare the total effective rate of homocysteine(Hcy), CystatinC(CysC), urinary albumin excretion rate(UAER), fasting blood-glucose (FBG), Two hours after a meal blood sugar(2hPG), Glycated hemoglobin(HbA1C), urea nitrogen(BUN), Creatinine(Scr). As indexes are compared, observe the TCM syndrome change of early diabetic nephropathy before and after the treatment. Results: The total efficiency of xihong kang prescription in treatment of diabetic nephropathy(III)in the treatment group is 87.5%. After the treatment of TCM syndromes in total, there were significant differences (P<0.05) in two groups. Compare Hcy, CysC, UAER, FPG, 2hPG, HbA1c, statistically, dates of the treatment group is superior to that in the control group (P<0.05 or P<0.01). After the treatment, compare BUN, Scr, statistically,there was no significant difference in two groups (P>0.05). Conclusion: (1) Through this research we found that the xihong kang prescription is an effective prescription in the treatment of diabetic nephropathy (phase III). The effect of traditional Chinese medicine with western medicine therapy is better than pure western medicine treatmentand the clinical symptoms obviousiy improved. (2) By probing Hcy CysC UAER we found that Hcy CysC UAER is superior to Cr in evaluating the index of damaged kidney function and it can be used to evaluate early renal damage of the convenience of the diabetic nephropathy accurate and reliable index. (3) Through the observation of the treatment group and control group, compare Hcy CysC UAER before and after treatment, we found that combined Traditional Chinese and Western Medicine treatment in early diabetic nephropathy has more
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advantages than the pure western medicine therapy.
A-046Clinical Analysis of 200 Cases of Curative Effect Factors on Treating Knee Osteoarthritis with Small Needle Knife
Ma Lei1, Meng Qingcai11Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: Validation small needle knife's effect on knee osteoarthritis, for the more, to discuss the different factors that is closely related with the result. Method: Choose 200 cases of knee osteoarthritis
patients， according to the standard who are only treated with small needle knife. After treatment, the
result would be observed four and eight weeks later. The factors influenced the effect of further line Logistic regression analysis. Results: The material treated with small needle knife after four and eight weeks, the total effective rate was 95.55% and 94.0% respectively, compared with before treatment, the difference was statistically significant (P<0.05); The Logistic regression analysis: there were four factors including disease course, weight, room number and deformed joint degree, prognosis for independent risk factors, and it is the heavier weight, the longer duration, the more serious deformed joint degree, and the more number of room, the worse effect; there were two factors X-ray classification and disease point number for prognosis of the independent protection factor: the result in the short term is when the
X-ray's change more serious，treatment of patients with more disease points, the curative effect will be
better. Conclusion: Needle knife is a kind of safe and effective treatment. According to each patient's different situation, make personalized treatment case, short-term curative effect is better, but the long-term efficacy needs further clinical validation and observation.
A-047The study of Clinical Curative Effect on Jing Yi An Shen Powder on 104 Cases of Insomnia Treatment by Acupoint Application
Wei Qing1, Hu Xiaoling1
1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: To observe the clinic curative effect of Jing Yi An Shen Powder by acupoint application on insomnia. Methods: In clinic study, 104 cases of insomnia were divided into three groups at random, experimental group (n=38), diazepam group (n=34) and bran control group (n=32). The experimental group and bran control group were treated with acupoint application method, while the diazepam group using drugs.7 days for a course, two weeks later, we observed the effect on patients. Results: 1. Jing Yi An Shen Powder improve the total effective rate of insomnia is 81.6%, while the diazepam group is
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82.4% and the bran control group is 34.4%. In the statistical analysis, the experimental group and diazepam group compared with the bran control group (P< 0.01), difference was statistically significant compared; the experimental group compared with diazepam group (P<0.01). there is not statistically significant.2. After the treatment，the experimental group、diazepam group and the bran control group in Epworth sleepiness total amount control comparing have statistical significance (P<0.01). 3. Jing Yi An Shen Powder acupoint sticking apply to improve sleep quality rate of 68.4%, diazepam group to improve sleep quality rate of 67.6%, bran control group to improve sleep quality rate of 27.3%.After treatment, the comparison between groups, the experimental group、diazepam group and the bran control group in Sleep efficiency, sleep disorders, daytime dysfunction and total comparing have statistical significance (P<0.05). 4. Jing Yi An Shen Powder acupoint sticking improve TCM syndrome effective rate is 86.8%, while the diazepam group is 52.9%, and the bran control group is 37.5%. In the statistical analysis, the experimental group compared to diazepam group and bran control group have statistical significance (P<0.05). After treatment, the comparison between groups, the experimental group compared to diazepam group and the bran control group in morning sleepy, palpitations yi jing, the mouth of the bitter dry throat, TCM symptoms integral falls down have statistical significance (P<0.05). the experimental group、diazepam group compared to the bran control group in limbs were heavy, forgetfulness, sweat out and total credit of TCM symptoms integral falls down have statistical significance (P<0.05).The experimental group compare to the bran control in Improve inattention aspects TCM symptoms integral falls down have statistical significance (P<0.05). Conclusion: Jing Yi An Shen Powder can effectively extend the sleep time, improve sleep quality, and improving associated symptoms, thus improve the clinical efficacy.
A-048Clinical Curative Effects on Repeated Shallow Puncture with Filiform-fire Needle on Herpes-zoster
AyijiamaliTuohuti1, Liu Hongxia1*1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: To observe the clinical curative effect of repeated shallow puncture with Filiform-fire needle on Herpes zoster, evaluation repeated shallow puncture with Filiform-fire needle is better than ordinary acupuncture praise treatments for the superiority of herpes zoster. Methods: 70 cases of herpes zoster were selected by the patient in hospital order Numbers, by means of random Numbers table into two groups. The treatment group for ordinary acupuncture, Repeated shallow puncture with Filiform-fire needle joint foundation treatment method; The control group for the common foundation with acupuncture therapy, and basic therapy for: Chinese medicine internal and (a tonic liver tonga diarrhea reduced), Chinese medicine collapse stains therapy Cooperate with cutem therapy. Ordinary acupuncture choose acupuncture point: local wai stab, ridge with side clip acupoint and Distal match point (TaiChong,YangLingQuan, LiangQiu,HeGu, ZuSanLi, SanYinJiao), Repeated shallow puncture with Filiform-fire needle is the third day of treatment, her lesions local use 0.25 x 0.25 mm of HaoZhen fast evenly spread thorn, straight into the straight out, slightly into the skin namely stop, then use the proper size cupping adsorption on treat place, leave cans of 5-10 minutes, the tank with disinfection
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after cotton wipe local, according to the need to get 2-3 days and then a treatment. Treatment in observing herpes number, erosion, erythema, edema and change; And in the third days before treatment, treatment and after treatment for pain score, record the time, began to blister scabby time, all the scab off time, half the time required for reducing pain, pain duration. Results: Finish the 70 patients with clinical curative effect and the control group had no significant differences (P > 0.05), two groups before and after treatment symptoms and signs giving a mark was not statistically significant (P > 0.05); The third day after treatment and therapy, the treatment group was better than control pain relief degree; 2 the blister, start time of time, all the scab off scabby time, reducing pain half the time required for constant pain, all have time significance, and the treatment group than in control group. Conclusion: cooperate with Repeated shallow puncture with Filiform-fire needle for Herpes zoster has good curative effect, relative to the control group can quick pain relief, accelerate the scabby herpes, shorten the time constant pain, enhance the curative effect, short treatment course, prevent the sequela of neuralgia, reduce cost, is safe and effective.
A-049Clinical Effect on DLK- A for Treatment and Prevention of Metabolic Syndrome (MS), on Blood Sugar, Blood Pressure, Blood Lipids and Weight
Palidan Wushuer1, Nurmuhammat Amat2*, Duo Likun3
1 Neurology Department, Affiliated Traditional Chinese Medicine Hospital ， Xinjiang Medical University, Urumqi-830000, Xinjiang Uighur Autonomous Region, PR China; 2Traditional Uighur Medicine Institutet, Xinjiang Medical University, Urumqi-
830011, Xinjiang Uygur Autonomous Region, PR China ;3 Salamat Biotechnology Co., Ltd. Xinjiang 830011, China ；*Correspondence should be addressed to [email protected]
Objective: By using medical laboratory technique, to evaluates the effectiveness of DLK in treating metabolic syndrome. Figure out and verify the effectiveness of DLK's on metabolic syndrome. Methods: Using double-blind contrast clinical test, Taking weigh index greater than 25 as a standard (BMI> 25), the volunteers were divided into experimental group and control groups applying DLK and placebo randomized, The main observation indexes were weight, FBG, fasting insulin level, blood pressure, blood lipids, HBALC and FMN. Various indicators were measured before and 30 days after the experiment. Compare the improvement of DLK and placebo on MS, by using medical laboratory technique to test the weight, FBG, blood pressure, blood lipids before and after the treatment. Results: According to statistical results, experimental group had significantly lower indicators than control groups in weight, FBG, blood pressure, CHOL, triglyceride, LDL and fasting insulin level(P <0.05). HINS is a manifestation of insulin resistance and insulin resistance is the main cause for MS. According to statistical results, and experimental group had significantly lower indicator than control groups in fasting insulin level, indicating DLK significantly improving insulin resistance consequently showing potential value of DLK in prevention and treatment of MS. Conclusion: DLK had decreased blood sugar, and blood pressure, blood lipids (including triglycerides and CHOL), especially weight. DLK, not only as a natural substance, is eligible to be developed into a functional food or nutritional supplement. But also have high safety and effectiveness.
A-050
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Clinical observations on chronic prostatitis of dampenss-heat down-flowing stasis with qianlieshuang decoction
Sha Mo1, Ma Chao1
1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
Objective: This thesis mainly discussed the research of Chronic Prostatitis caused by the inflammatory of dampness-heat down-flowing through the treatment of Qianlieshuang Decoction by the way of observing the curative effect and the influence of the observation index and meanwhile made a contrast treatment with Qianlietongyu Capsule. Also with the purpose of exploring the therapeutic action of Qianlieshuang Decoction at the treatment of Chronic Prostatitis, and providing clinical evide-nce for the Chinese Medical Treatment. Method: With the randomized, controlled method, 80 Chronic Prostatitis patients that caused by the inflammatory of dampness-heat down-flowing were selected, 40 in the treatment group (Qianlieshuang Decoction) while 40 in the control group (Qianlietongyu Capsule).Observed the symptom score of treatment of Chinese Medicine, NIH-CPSI score, prostate fluid routine examination of changes in indicators before and after the treatment .Do the statistical analysis and draw the conclusion. Result: Treatment group: clinical cure: 11 cases, significantly effective: 14 cases, effective:10 cases. the clinical cure rate, the significantly effective rate, the effective rate and the total effective rates were 27.5%、35.0%、25.0%、87.5%.Control group: clinical cure:5 cases, significantly effective:11 cases,effective:8 cases. the clinical cure rate, the significantly effective rate, the effective rate and the total effective rates were12.5%、 27.5%、 20.0%、 60.0%. The treatment group is significantly better than the control group at comparison of the symptom score of treatment of Chinese Medicine, NIH-CPSI score, prostate fluid routine examination with the result of P<0.05. Conclusion: Qianlieshuang Decoction has significant function on the treatment of Chronic Prostatitis caused by the inflammatory of dampness-heat down-flowing, concentrated expressed in the improvement of symptoms and Laboratory tests index, and has not found side effects yet. This kind of treatment Is a relatively safe, economical and effective treatment methods.
A-051Clinical Study on combination of Traditional Chinese Medicine and Acupuncture of Promoting the Recovery of Gastrointestinal Function in the Treatment of Postoperative Patients with damp-heat typeGuli Shaera1, Liu Zhiyan1 1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: To observe the clinical efficacy of acupuncture combined with drug on recovery of gastrointestinal function of damp-heat in the abdomen after surgery, and to explore effective methods of acupuncture combined with drug to treat gastrointestinal disorders after damp-heat pattern abdominal
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surgery. Methods: 65 patients of damp-heat pattern after abdominal surgery in accordance with the principle of randomized controlled were divided into 34 cases of control group and 31 cases of treatment group. The control group was given oral formula, twice a day, for 7 days totally; treatment group on this basis, receive acupuncture treatment in the morning of the second day after surgery, once a day, for 7 days totally also, evaluate the efficacy of the two groups at 7 days after surgery. Compare the two groups of patients with recovery of gastrointestinal function of time after surgery, the common clinical symptom score changes, the efficacy results, the VAS pain score scale (VAS) and peripheral white blood cells before and after treatment. Result: (1) treatment group, the first bowel sound and the first exhaust defecation time than the control group was significantly shorter, the difference between the groups was statistically significant (P<0.05). (2) The analgesic effect of the treatment group was better than the control group, significant differences between the groups (P<0.05). (3) treatment group in improving anorexia, abdominal distention, abdominal pain, defecation difficulties in clinical symptoms was better than the control group, the difference between the groups was statistically significant (P <0.05). (4) Control group, the total effective rate was 66.7% total effective rate of 80% was statistically significant (P <0.05). (5) Treatment group after 5 days in peripheral blood neutrophils compared with control group decreased, the treatment group after 5 days in peripheral blood lymphocytes than in the control group increased, the difference between the groups was statistically significant (P≤0.05). Conclusion: Acupuncture treatment can shorten the patients after discharge, bowel movement time; the oral combination of traditional Chinese medicine also has analgesic and improves clinical symptoms and other effects, and postoperative peripheral white blood cell changes in bidirectional positive regulatory role. A-052Decoction of Bushentongluo and its Decomposed in Repair of Articular Cartilage Defects in Rabbits
Li Leijiang1, Lu Yong1
1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: The treatment of the role of research Bushentongluo decoction and its decomposed decoction on rabbit articular cartilage defects repair，Clear the "decoction of Bushen", "decoction of Tongluo" and "decoction of Bushentongluo” whichever is more effective in repairing articular cartilage defects treatment. Methods: Take the drilling of 60 New Zealand white rabbits with left knee medial femoral condyle center, diameter 3.5mm, depth of 3mm, up to the subchondral bone, resulting in full-thickness articular cartilage injury. After surgery will be randomly divided into five groups (n=12). Group A: Decoction of Bushentongluo to cooperate with the hyaluronic acid; group B: Decoction of Bushen to cooperate with the hyaluronic acid; group C: Decoction of Tongluo to cooperate with the hyaluronic acid; Group D: hyaluronic acid group; group E: model control group. After the modeling, the A, B, and C were given Chinese medicine decoction 3mL / kg orally; A, B, C, D, four groups a week after hyaluronic acid gel 0.5ml line puncture of the left knee joint cavity, once a week for five weeks in a row; special treatment of the E group did not. 8 and 12 weeks after surgery, respectively in each group were sacrificed 4, cut a left knee medial femoral condyle observed defect in cartilage repair, parallel HE
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staining, Safranin O staining and immunohistochemical staining, light microscopy the shape of the cartilage defect repair, the level of expression of Collagen II histomorphometry score. Take 12 weeks after cartilage specimens by transmission electron microscopy observation. Results: After four weeks in each group the cartilage defect repair tissue is not obvious morphological different. 8 weeks after surgery in each group the cartilage defect repair tissue morphology. After 12 weeks in group A cartilage defect repair tissue surface is smooth, hyaline cartilage cells, immunohistochemical staining strongly positive. Group B and Group C defects chondrocytes increase in the number of close to normal mature cells; the overall situation compared with group A poor. Group D defect repair tissue is mostly fibrous tissue, a small amount of cartilage cells. Rough group E defect repair tissue, and more fibrous tissue, A combination of two scoring after 12 weeks A, B, C, three sets of scores were significant difference with Group D score, P<0.05; B;C, the score of the two groups with Group A score significant difference, P<0.05; group B and group C scores were the number was no significant difference, P> 0.05.Conclusion: The decoction of Bushentongluo, the decoction of Bushen and the decoction of Tongluo have a significant therapeutic effect to cartilage defects, the efficacy of Bushentongluo is slightly better than the decoction of Bushen and the decoction of Tongluo, decoction of Bushen and decoction of Tongluo had no significant effective differences.
A-053Effect of Jangzhuoshengqingtang on Pathology organization morphological、plaques area and Inflammatory Reaction on Atherosclerotic in Aorta of Apo-E-gene knockout Mice
Wang Jipeng1, Xin Xiaoyi11 The First Affilated Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
Objective: To use Apo-E gene knock out mice atherosclerosis modeling jiang zhuo sheng qing tang of turbidity atherosclerosis inflammatory reaction mechanism, effect intensity evaluation. Research jiang zhuo sheng qing tang of turbidity As the main function of the inflammatory response targets, and regulation of the protection mechanism of the link. Methods: Thirty-five Apo-E knockout mice were fed a high-fatdiet for 6 weeks. Five mice were chosen and stained with HE until the atherosclerotic plaques formed. they were randomly divided into three groups:
model group(jiang zhuo sheng qing tang) ， Chinese medical control group(bie jia ruan gan
pian) ， medical control group(withsimvastain). Three groups were threaded with respective
drug in clinical recommended for another 6 weeks accompanied by feeding common diet. The
changes of IL-6、TNF-a in serum and plaques area in Atherosclerotic were detected. Results:
A11 the groups could decrease the level of serum IL-6 、 TNF-a ， slow the atherosclerosis
development degree and reduced the plaques area，and the effects in jiang zhuo sheng qing
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tang group and with simvastain group were better than that in bie jia ruan gan pian group ；And they could reduce the lipid as well as the lipid to collagen content in plaque. Conclusion: Jiang zhuo shengqing tang has the turbidity atherosclerosis function, its mechanism may cut IL-6, TNF-a expression of the relevant.
A-054Experimental Study of Effect on Aplastic Anemia Model Rat’s Regulation Factors IL–2、TNF–a Treated with Tonifying Kidney and Replenishing Essence Method
Xie Rong1, Shan Lijuan1
1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi, Xinjiang Uighur
Autonomous Region, China
Objective: To observe the effect of Aplastic Anemia the model rat’s Regulation Factors’ level treated with Tonifying Kidney and Replenishing Essence Method, investigate the mechanism of action. Methods: Clean-class Wistar rats were divided into 4 groups， namely blank group, model group, treatment group (Tonifying Kidney and Replenishing Essence basic prescription, TKRE) and control group(Aplastie Anemia Blood-engendring tablet) respectively. Rats were given the 5-FU plus Myleran to induce AA model of rats. The blank group rats were fed normal foods, after judge AA model succeed, the treatment group and control group rats were fed with TKRE prescription and Aplastie Anemia Blood-engendring tablet for 30 days, After the experiment ended, to observe each group of rat’s general condition and symptoms, test each rat’s circumference blood cytometry, the marrow pathology and the level of IL–2 and TNF–a. Results: (1)Rat’s general condition and symptoms: Compared with model group, the treatment group and control group rat’s spirit diet and motion were improved, quality of life raised; (2)Circumference blood cytometry: Compared with model group, the numbers of blood cells in treatment group were increased in varying degrees, the difference were statistically significant (P<0.05).Compared with control group, the treatment group has significantly rise in white blood cell, the blood platelet and the net weaves the red blood cell number, the difference were statistically significant(P<0.05). (3)Marrow pathology: Compared with the model group, the treatment groups' marrow proliferation restored, the three System cells proliferate, un-hematopoietic tissue and adipose Tissue reduce relatively, the hematopoietic structure restored, the treatment group bring about a striking effect than control group. (4)Cell Factors IL-2 and TNF-α: IL-2 and TNF-α levels were significantly elevated in model group and treatment groups than the blank group. Compared with model group,IL-2 and TNF-α levels were declined in treatment group, the difference was statistically significant(P<0.05).Treatment group compared with control group, the IL-2 has difference in the two groups (P<0.05), the TNF-α has no difference in the two groups (P>0.05). Conclusions: According to the experiment show that the tonifying kidney and replenishing essence method could significantly improve rat’s general condition and symptoms, promote rat’s circumference blood cells, improve bone marrow hematopoietic function of aplastic anemia model of rat, Take into account that the tonifying kidney and replenishing essence method can effectively improve abnormal secreting of regulation factors, encouraged the bone marrow hematopoietic function’s recover, through achieving the therapeutic purposes.
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A-055Literature Research on Prescription Rule of Oral Drugs for Osteoarthritis
Chu Litao 1, Lu Yong1*1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi, Xinjiang Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: To explore the application range and prescription rule of the oral drugs of GuBi in Ancient and modern literature in the different periods, And further analyze its pathogenesis and its therapeutic treatment. For future clinical and scientific research, to provide document foundation and more explicit medication guide. Methods: Investigate mainly the oral drugs of osteoarthritis literature material of Traditional Chinese Medicine from the Qin and Han Dynasty to the modern times, and identify, reorganize the original documents. There are 44 1topical prescription in this study, Each other medicine information applications frequency analysis, variance analysis, cluster analysis, mathematical statistics method and combining analysis induction, formula metrology research methods, party card corresponding methods GuBi for use in formula of law of research and analysis . Results: Cumulative frequency of Chinese herb reaches93. 69%, which are for restoratives Chinese Medicinal Herbs, Chinese Medicinal Herbs for invigorating the blood and removing blood stasis, Antirheumatic Chinese Medicinal Herbs, diaphoretics or exterior syndrome relieving Chinese Medicinal Herbs, warming the internal, Chinese Medicinal Herbs for promoting diuresis and resolving dampness, heat clearing Chinese Medicinal Herbs, Chinese Medicinal Herbs for calming the liver to stop endogenous wind, qi regulating drugs and antitussive and antiasthmatic Chinese Medicinal Herbs are the main treatment drugs for osteoarthritis. They are the main drug categories warm, neutral and cold and the most that accounted for 88. 77% in four nature. Sweet pungent and bitter are the most that accounted for 93. 40% in the five flavors. Cumulative frequency of liver, heart, kidney, spleen and lung is 91.14%, Through the system clustering formed 10 clustering prescriptions . There are different in efficacy, four climates, the five kind flavors and pertaining meridian . Conclusion: 1. The prescription rule of the oral drugs of GuBi are as follows: (1) Used in conjunction treatment of Xinkai Kujiang and Ganwen Bingshi; (2) Adjusting viscera placidly to get rid of pathogens; (3) Fill in the diarrhea, diarrhea in complement, (4) insect drugs significant effect. (5) The vines of the genus, pass through into the network. 2. General use in gansu and Michael essien, taste bitter primarily, medicinal properties to warm, neutral, cold is given priority to, be in by the liver, kidney, the heart sutra by the lung, spleen, by primarily, focuses on the liver and kidney spleen. Drug class to make up for restoratives Chinese Medicinal Herbs, Chinese Medicinal Herbs for invigorating the blood and removing blood stasis, Antirheumatic Chinese Medicinal Herbs primarily. 3. Through the prescription that the osteoarthritis of basic prescription type has five: Respectively for FengHanshiBi type, FengShiReBi type, TanShiNeiZu type, GanShenKuiXu type. 4. Virtual, blood stasis, phlegm, Pathogenic is the main factor of the disease, Total treatment on having to WenTong therapeutic mainly, and concurrently to remove stasis expectorant, dispelling rheumatic and tong collaterals. 5. Through the analysisi of the 10 cluster prescriptions, special drug on the following categories: Rectifying the drug Chenpi Banxia, Qianghuo Duhuo, Duhuo Xixin, Rectifying and Centralizer drugs:Gouji Gusuibu, Chishao Baishao, Shengdi Mudanpi, Fuzi Ganjiang. Centralizer
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drugs:Huangqi Baizhu, Tusizi Gouqizi, Fuzi Rougui, Danggui Shudi, Danggui Chuanxiong.
A-056The Research on the Primary Immune Thrombocytopenia of Different Syndrome Types and Characteristics of Platelet Parameters
Wang Yahong1, Bai Yusheng1
1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: To discuss the relation of different Syndrome type and platelet parameters in Primary immunity thrombocytopenia (ITP) and seek the reference for objective target of TCM (traditional Chinese Medicine) syndrome type and curative effect. Methods: To make better observation table of clinical cases, 126 patients with ITP were in accord with standard and the basic information, laboratory examination indexes, clinical symptoms, tongues, and pulses in these patients were collected before treatment. The tester judged syndrome type in ITP patients by unified standard，collected platelets parameters and analysed the relationship between TCM syndrome type and platelet parameters. Result: The ages in Patients among the types in blood heat, yin deficient, and blood stasis impedance type showed statistically significant differences (P<0.05); In the distribution of TCM syndrome types, the largest number of types was failure of qi to check blood and yin deficiency, but the blood heat and blood-stasis obstruction accounted for relatively small percentage of all types. The platelet count and thrombocytocrit in different types among blood-stasis obstruction, blood heat, yin deficiency and failure of qi to check blood were statistically significant differences (P<0.05);The mean platelet volumes in different types among blood-stasis obstruction, blood heat, yin deficiency and failure of qi to check blood were statistically significant differences (P<0.05);The platelet distribution widths in different types among blood-stasis obstruction, blood heat, yin deficiency and failure of qi to check blood were statistically significant differences (P<0.05);Conclusions: The syndrome type of TCM of ITP and platelet parameters have significant difference and platelet parameters provide objective reference for TCM syndromes of ITP; the correlation between TCM syndrome and platelet parameters predict different syndrome types of TCM have a certain reference value towards the prognosis of efficacy
A-057The research on Qufeng antispasmodic to asthma scattered (wind phlegm wheezing) patients with pulmonary function, FeNO influence
Bai Wenmei1, Li Fengsen1*1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China； *Correspondence should be addressed to [email protected]
Objective: To observe the antispasmodic for chronic scattered bronchial asthma duration wind phlegm wheezing patients with pulmonary function, breathe the influence of nitric oxide. Methods: 60 patients
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with chronic duration with bronchial asthma wind phlegm wheezing patients were randomly divided into the treatment group and control group of 30 cases of 30 cases, both groups to hormone inhaled treatment, the treatment group and give oral qufeng antispasmodic scattered. 8 weeks of treatment. Observe both groups of patients before and after treatment symptoms, signs, pulmonary function, breath nitric oxide changes. Results: treatment group patients before and after treatment mean the FEV1 % were 77.13%, 93.34%, and had more significant statistical difference (P<0.01), and the control group of patients before and after treatment FEV1 % were 84.56%, 73.87%, had significant both statistical differences (P < 0.05); The treatment group of patients before and after treatment mean FeNO were 63.04 ppb, 32.17 ppb, had more significant statistical difference (P<0.01), and the control group of patients before and after treatment mean FeNO were 59.04 ppb, 55.02 ppb, had no significant statistical difference (P > 0.05). Conclusion: qufeng antispasmodic scattered chronic duration can improve the bronchus asthma wind phlegm wheezing of patients with lung function, can reduce the duration asthma patients airway inflammation, bronchial hyperresponsiveness and breathe nitric oxide level.
A-058Research on Catharsis and Moisturizes Theoretical Literature of Traditional Chinese Medicine
Chen Lu1, Zhou Guang1
1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
Objective: This issue through the collation of ancient and modern skin-related literature of Traditional Chinese Medicine. Combined with the statistical analysis of the common skin disease related syndromes, pathogenesis, legislation and prescription drug laws in the medical records of dynasties masters, To comb and clarify the skin Catharsis and Moisturizes physiological basis ,functional performance and the relationship among Ying -Wei ,zang fu and choroid; and its dysfunction pathogenesis characteristics, Summed up the legislation and prescription medication law based on the theory of Catharsis and Moisturizes, Provide the basis for the establishment of Chinese medicine dermatology disciplines Catharsis and Moisturizes theoretical system. Method: Use of systematic literature research methods, to collect and record theoretical basis of skin-related information from the Qin and Han Dynasty to the modern times, and identify, reorganize the original documents. To establish the theoretical basis of Chinese medicine skin database; cluster, stratified sampling to select the various periods representative of common skin disease masters medical records,with prescription metrology and frequency analysis, analysis of variance for statistical analysis of the literature information. Results: A total collection of skin theoretical basis of literature information 1996,363cases of common skin disease medical records information.The organizational structure of the Chinese medicine skin including striae, hairs, XuanFu, fur, claws, coetaneous region, FuLuo, etc; the physiological basis and functional performance of the Chinese medicine skin including excretion of sweat, the catharsis conditioning internal and external qi, to clear the blood and body fluids, evacuation exogenous pathogens, adjust the cold and heat, stirs the body fluid to moisten the skin; in these cases, skin catharsis and moisturizes dysfunction in syndromes and pathogenesis of skin disease have universal and characteristic; Famous
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Medical Records legislation and prescription drug based on the catharsis and moisturizes there was a marked convergence; pruritus, desquamation, flush, swelling, ache, rough, hypertrophy, exudation, drying is the most common symptom of skin disease, pathogenic factor lag skin, skin dry and heating is the most important partial pathogenesis of skin disease; catharsis, heat clearing and pathogenic expelling; moisten is the common rule for treat skin disease; clearing away heat and damp, clearing camp and cooling blood, dispelling wind and heat is the main treatment methods of skin disease .Heat clearing Chinese Medicinal Herbs, restoratives, diaphoretics or exterior syndrome relieving Chinese Medicinal Herbs, Chinese Medicinal Herbs for promoting dieresis and resolving dampness，Chinese Medicinal Herbs for invigorating the blood and removing blood stasis, Chinese Medicinal Herbs for calming the liver to stop endogenous wind, qi regulating drugs and Anti-rheumatic Chinese Medicinal Herbs are play a major role in the clinical treatment. Cold､warm and neutral are the most in four nature; Bitter, sweet and pungent are the most in the five flavors; meridian mainly in the liver、 heart、 lung、 spleen and stomach. Prescriptions are different in the five kind flavors and pertaining meridian by drug administration in the ancient、 modern and periodicals. Conclusion: Catharsis including skin permeate and opening and closing, and the physiological status and functional performance of Moisturizes including skin moist and body fluid, blood compresses. Catharsis and Moisturizes is specifically reflected in the form of the wei external function of skin, is also the functional performance on the skin effect of Ying -Wei ,zang fu and choroid. Its dysfunction is not only a skin disease main pathogenesis, but also the theoretical basis of the legislation and prescription medication.
A-059The influence of Spleen detoxification soup for murine spleen deficiency type psoriasis ET-1, E-selectin and of PCNA
Guo Shanshan1, Liu Hongxia1* 1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: Observation of the spleen jiedu decoction of pixu psoriasis sample mouse models of vascular active factor and vaginal epithelial PCNA expression, this essay discusses the spleen decoction in the treatment of detoxification of pixu wet type filled with psoriasis vulgaris mechanism. Methods: The subject of three experiments, each experiment was randomly divided into control group, saline group，A capsules, spleen detoxification soup, low-dose group. Modelling for dual model, and 1-4 days for the model of mouse spleen deficiency, 5-7 days while modelling in Spleen psoriasis-made mold, 8-13 days for the drug or placebo intervention stage, 14 days sampling. To verify the Spleen model with psoriasis model is successful, required from the blank group and the saline group in each experiment, randomly selected 12 mice, the serum melatonin (MT) determination and vaginal epithelial cells cell mitosis observed. After successful modelling on Spleen psoriasis mouse model of plasma endothelin-1, soluble E-selectin were detected elements and vaginal epithelial PCNA. Result: 1、A saline serum melatonin (MT) level lower than the blank group, the two groups P<0.05， from the laboratory point of view prompted spleen deficiency model was successful. 2、vaginal epithelial cells of normal saline group of mice the number of mitotic higher than the blank group, suggesting that psoriasis successful modeling.3、Spleen detoxification soup to reduce the deficiency of the spleen psoriasis-like
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mouse model of plasma endothelin-1， soluble E-selectin in the role. Blank group and the saline group P <0.05；A capsules and spleen detoxification soup, low-dose group compared with the saline group P <0.05，A capsules and spleen detoxification soup high in the low dose group P> 0.05. Spleen detoxification soup, low-dose group P> 0.05 .4、spleen detoxification soup reduce the role of spleen deficiency type psoriasis mouse model of vaginal epithelial PCNA expression. Between the groups overall P <0.01，X 2= 43.815 by pairwise comparisons between the sample Wilcoxon rank sum test (Nemenyi), a blank group and the saline group P <0.01 with acitretin capsule group, the spleen detoxification soup in the high dose group compared with P> 0.05, saline group with acitretin capsule group, spleen detoxification soup low in the high dose group pairwise comparisons P <0.01， A capsules, and spleen detoxification soup is low, middle and high dose groups pairwise comparisons P> 0.05. Conclusion: The spleen jiedu decoction on plasma endothelin 1 and soluble E and choose PCNA expression with benign adjust action, by lowering endothelial cell activation degree, restrain psoriasis lesions formed in capillaries, reduce the cutin form abnormal cellular proliferation and differentiation and so on various reach the purpose of psoriasis treatment.
A-060The Comparison on the Location of Insomnia According to Different Classification of Traditional Chinese Medicine of Five-god Theory and Four Examinations
Fan Xiufang1, Zhang Xingping1
1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China
Objective: Study Chinese medicine insomnia five-God type and combination of all the typing consistency in the positioning of the five internal organs, and provide clinical evidence of genotyping diagnostic method of Traditional Chinese Medicine Insomnia five God. Methods: Subsidiary of the Chinese Medicine Hospital sleep specialist expert outpatient collection of Xinjiang Medical University of insomnia in patients with 204 cases, standardized collection of clinical information collection form, were the five gods of the traditional Chinese medicine insomnia type and combination of all type, insomnia disease established by genotyping the main orientation of the five internal organs, the establishment of information database, the use of Kappa test methods to compare the type of Chinese medicine insomnia five God with four diagnostic combination of all type consistency in the positioning of the five internal organs. Results: the consistency of the five gods of the traditional Chinese medicine insomnia type and combination of all typing on the five internal organs, Kappa, conformance test, kappa = 0.837 (P <0.001). Conclusion: The type of Chinese medicine insomnia five God with four diagnostic parameter sub-type high consistency in the positioning of the five internal organs.
A-061The comparison research of acupuncture and acupoint application to patients with AECOPD as well as the influence of lung function and life quality
Shao Meimei1, Liu Zhiyan1
1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
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Objective: To observe influence of acupuncture and acupoint application therapy in lung function of patients with acute exacerbation of chronic obstructive pulmonary disease compared to the improvement of clinical symptoms, quality of life and difficulty in breathing. Methods: 60 patients who in accordance with the inclusion criteria were divided into four groups, including the control group (non-acupoint acupuncture and simulated acupoint application)、 acupoint application group (plus non-acupoint acupuncture)， acupuncture group (plus simulated acupoint application), acupuncture plus acupoint application group，each group has 15 cases. The four groups were treated with conventional Western medicine treatment, once a day, and 15 days as a course of treatment. Observed and record before and after treatment respectively, which are lung function index (FEV1/FVC, FEV1%)、TCM symptom score、 quality of life score (CAT scale)、 difficulty in breathing of grading (MRC scale). Results: the group of acupoint application in improving cough, expectoration, wheezing was statistically significant (P <0.05); acupuncture group in improving cough, expectoration, wheezing, shortness of breath aspects are statistically significant (P<0.05); and two associated with a significant effect (P <0.01);In aspect of improving lung function in AECOPD patients, the quality of life scores, difficulty in breathing scores: acupuncture group and acupoint application group after treatment, both improved (P <0.05), however, the effect of acupuncture combined with acupoint application group is the best (P<0.01), and better than the other three groups. Conclusion: The results confirmed the effectiveness of acupuncture and acupoint application in the treatment of AECOPD patients, showed that both improve pulmonary function in patients, to improve the acute symptoms、 breathing difficulties and improve the quality of life is meaningful, and combination of two is more effective than simple
acupuncture and acupoint application. Take Western medicine treatment of chronic obstructive
pulmonary disease as a base, on the use of acupuncture, acupoint application therapy can not only improve the clinical efficacy but also convenient and safe.
A-062The effect of jianpijiedu decoction on the vascular active factor of psoriasis model mice
Gao Yuan1, Liu Hongxia1*1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: To observe the plasma endothelin-1 and serum soluble E - select element level after giving jianpijiedu decoction to psoriasis model mice and to discuss the effect and mechanism of jianpijiedu decoction for curing psoriasis so as to provide experiment basis for clinical applications. Method: In experiment 1, using the radiation immune method for determining the content of plasma endothelin-1, the psoriasis model mice were divided into blank group/ model group /Chinese medicine group and control group, in addition to blank group, all the groups are injected Diethylstilbestrol Diphosphate Inj three consecutive days, then giving the Sodium Chloride /jianpijiedu decoction and Acitretin capsules to blank group, Chinese medicine group and control group respectively for 6 days , to use pick eyes method to take about 1 ML blood for determination before lethal injection of colchicin. In experiment 2, using the ELISA method for determining the content of Serum soluble E - select element, the model mice were divide into blank group/ model group /Chinese medicine group and positive control group, in
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addition to blank group, all the groups are injected Diethylstilbestrlo Diphosphate Inj three consecutive days, then giving the Sodium Chloride /jianpijiedu decoction and Acitretin capsules to blank group,/Chinese medicine group and positive control group respectively for 6 days , to use pick eyes method to take about 1 ML blood for determination before lethal injection of colchicin. Result: In two experiments, both jianpijiedu decoction and Acitretin capsules can reduce plasma endothelin-1and soluble E - select element level ,Chinese medicine group was better than model group(P<0.05), positive control group was better than model group(P<0.05), model group was better than blank group(P<0.05), There was no significant differences between the Chinese medicine group and Acitretin capsules group in reducing plasma endothelin 1 level.（P>0.05）F:\Dict\resultui\queryresult.htmlConclusion: The effect of jianpijiedu decoction in treating psoriasis might by means of blocking multiple Onset link, so the content variation of Plasma endothelin-1 and serum soluble E - select element may serve as Pharmacodynamic index to provide experiment basis for clinical applications.
A-063The Experiment of Jian Pi jiedu decoction on Guinea Pig Model of Psoriasis with Spleen Deficiency Syndrome
Guo Fei1, Liu Hongxia1*1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: To establish the Guinea Pig Model of Psoriasis with Spleen Deficiency Syndrome and study the function as well as dose-effect relationship of jianpijiedu decoction. Methods: Guinea Pig were divided into 8 groups by randomization, blank 1group and blank 2group,experimental 1group and experimental 2group with 10 Guinea Pig in each group; Chinese herbs high dose ,the medium-dosage group and low dose groups, control group, with 12 Guinea Pigin in each group. Except blank 1 group and blank 2group, the experimental 1group established the psoriasis model with 5% propranolol emulsion, other groups after successfully establishing the psoriasis model with 5% propranolol emulsion, choose to give gavage of bitter and cold purgative rhubarb twice a day for a continuous duration of 7d to injure the spleen and stomach in order to duplicate a clinical like spleen deficiency model, then with ELISA test kit for the guinea pig serum TNF alpha IL-20 content changes, conduct visual inspection and pathological section observation on ear skin lesions among guinea pigs of all groups. Result: (1) 5% propranolol emulsion brought out thepsoriasis-like lesion ,after established the model, experimental 1group and blank1group has significant difference on baker grade (p<0.01);after give gavage of rhubarb, experimental1 group and blank 1group has significant difference on spleen deficiency syndrome grade and weight（p<0.01） ;(2)jianpijiedu decoction has effect on guinea pig serum TNF alpha and IL-20 content, after give gavage of jianpijiedu decoction and Acitretin capsule respectively, the serum TNF alpha and IL-20 content of the high dose and the medium-dosage Chinese herbs groups became lower compared with experimental 2 group （ p<0.01 ） ,the varying concentrations of the herbal medicine showed the obvious dose-effect relations.(3)jianpijiedu decoction has effect on psoriasis-like histopathological and ,after give gavage of jianpijiedu decoction and Acitretin capsule respectively, the lesion histopathological were improved to some extent of the three Chinese herbs and control group and there has significant difference compared with experimental2 group （p<0.01）,the result showed the higer of the dose and the better of the effect, and the Chinese
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herbs high dose group better than control group（p<0.05）(4) jianpijiedu decoction has improvement on Spleen Deficiency Syndrome, control group and experiment 2 group has no significant difference on
weight（P>0.05） ,three Chinese herbs has significant difference(p<0.05 ） . Conclusion: (1) The
psoriasis spleen deficiency syndrome group preliminarily has the pathological features of psoriasis, which are similar to TCM spleen deficiency syndrome symptoms.(2) jianpijiedu decoction has effect on psoriasis may related control its inflammatory reaction and immune adjustment;(3)jianpijiedu decoction not only improve the psoriasis-like lesion but also improve the spleen deficiency syndrome.
A-064The Experimental Study of Huangqi Guizhi Wuwu Decoction therapy on Failed Back Surgery Syndrome
Liu Kaiying1, Huang Yifei11Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
Objective: To study the effectiveness and mechanisms of Huangqi Guizhi Wuwu Decoction on preventing post laminectomy fibrosis and adhesion and to prevent failed back surgery syndrome effect. Methods: 95 adult rats were divided randomly into five group, each 19. A group: Nacl group, B group: Huangqi Guizhi Wuwu Decoction group, C group: Huangqi Guizhi Wuwu Decoction Plus invigorating the blood and removing blood stasis Chinese Medicinal Herbs group, D group: Huangqi Guizhi Wuwu Decoction group Plus resolving dampness Chinese medicine Herbs group, E group: Huangqi Guizhi Wuwu Decoction Plus resolving dampness and activating blood circulation Chinese medicine Herbs. All the rats were established as the experimental models undergoing L1 laminectomy with 0.3cm×0.8cm dura mater exposed. All laminectomy levels were treated with relative medicine for four weeks: In the treatment of at least 4, 8, 12 weeks, each group were sacrificed 7. To take paragraph spine with Lawson standards to evaluate adhesion degree and detect the content of hydroxy proline of the animal epidural in the scar tissue, and the groups were compared. Results: Compared with A, content of general observation in group E group A degree of adhesion significantly increased in 4, 8, 12 weeks, a significant difference(P<0.05). Compared with A, content of hydroxy proline of the epidural in the scar tissue of B, C, D, E group were decreased within 8、12 weeks were significantly different(P<0.05); At the 12th week，E group of hydroxy proline content not only less than A group(P>0.05) and B,C,D groups also significant differences(P>0.05).Compared with the 4th week, the content of hydroxy proline of the epidural in the scar tissue within 8, 12 weeks were significantly different (P<0.05). Conclusion: The drugs of Huangqi Guizhi Wuwu Decoction could reduce scar formation and fibroblasts, lessen fibro scar and inhibit the formation of adhesion in rats.
A-065The Impact of Yangyin Huowei Heji on Chronic Atrophic Gastritis Patients' Gastro scopeHistopathology
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Ding Guoning1, Zeng Binfang1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region
China; *Correspondence should be addressed to [email protected]
Objective: By using scaling biopsy technique, this thesis evaluates the effectiveness of Yangyin Huowei Heji on chronic atrophic gastritis objectively. Figure out Yangyin Huowei Heji's effection on chronic atrophic gastritis treatment, and verify the effectiveness of Xinjiang traditional Chinese medicine on chronic atrophic gastritis. Methods: Using a randomized controlled trial, in the group of patients with chronic atrophic gastritis, divided them into treatment group and control group. The treatment group was given oral self Yangyin Huowei Heji, the control group was given an oral Weifuchun pills. Comparing the improvement of Yangyin Huowei Heji and Weifuchun on chronic atrophic gastritis by using endoscopy, histopathology and Hp before and after the treatment. Results:1.In gastroscopic observation, the two groups in gastroscopy stomach mucosa degree has statistics difference after treatment (P<0.05); but the treatment group and the control group comparison showed no significant difference (P >0.05);2.In histopathology, two groups of patients with gastric mucosal degree, the extent of intestinal metaplasia and dysplasia degree showed statistical difference after treatment (P<0.05), the two group in improving glandular atrophy is also had statistical difference (P<0.05); 3. The Hp of the treatment group and the control group has significant difference after treatment (P<0.05); but comparison between the two groups shows no statistical difference (P>0.05). 4.In the comprehensive curative effect, total effective rate of the treatment group is 79%, the control group is 73%, comparison between the two groups shows statistical difference (P<0.05);5.Symptoms and signs of treatment group prove efficiency with no statistical difference (P>0.05). Symptoms and signs of control group prove efficiency, there was statistically significant difference (P<0.05). Conclusion: Yangyin Huowei Heji can not only effectively treat chronic atrophic gastritis and be resistant to Hp infection, but also can repair of gastric mucosa.To a certain extent, it also can reverse glandular atrophy, intestinal metaplasia and dysplasia, which can also prevent chronic atrophic gastritis from developing to cancer. In different syndrome types, Yangyin Huowei Heji has a satisfactory therapeutic effect. The control group in the stomach yin is deficiency, and in blood stasis of stomach meridian treatment effect is poorer.
A-066The Research on the Inhibition of Invasion and Metastasis of Colon Cancer Cells HCT116 after the Administration of YiQiFuShengFang by ERK/MAPK Signaling Pathway
Han Jing1, Lv Shuqin1
1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
Objective: To research the interaction mechanisms of anti-invasion and metastasis of tumor after using YiQiFuShengFang on HCT116 though Mitogen acttiveated protein kinase /Extracellular signal-regulated kinase signaling pathway, providing some basis of scientific experiments of anti-invasion and metastasis of tumor in using Traditional Chinese Medical prescription. Methods: The proliferation of colon cancer cells HCT116 was observed by methylthiazolyl tetrazoliym (MTT) assay. Select the appropriate drug density to carry on the next step of the experiment. The effect of the migration of HCT116 cells that treated by YiQiFuShengFang was examined by Scratch test. ELISA method was adopted to test the
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express of vascular endothelial growth factor (VEGF) on HCT116 cells after using drugs. ELISA method was adopted to test the express of Matrix metalloproteinase-2(MMP-2) on HCT116 cells after using drugs. The cell cycle and apoptosis of HCT116 cells that conducted by YiQiFuShengFang were examined by Flow Cytometry(FCM). P-ERK1/2 expression was determined by Western-blot, in that was the activation of ERK1/2 which was measured by the extent of ERK phosphorylation. Results: MTT assay showed the proliferation of colon cancer cells HCT116 could be inhibited by YiQiFuShengFang. YiQiFuShengFang inhibited the migration of HCT116 cells. YiQiFuShengFang reduced the expressing of VEGF, meanwhile,it cut down the expressing of MMP-2. Through the analysis of flow cytometric, the apoptosis of tumor cells were significantly higher and cell cycle was changed. Western-blot assay showed that ERK1/2 phosphorylation was suppressed by YiQiFuShengFang, that is ERK1/2 could be inactivated by the down regulation of P-ERK1/2. Conclusion: YiQiFuShengFanginhibits the proliferation of colon cancer cells hct116，down-regulates the expression of VEGF and MMP-2， and induces the apoptosis of cancer cells which may be mediated by MAPK/ERK signal pathway.
A-067The Study on Therapeutic Effect of Inflammatory Factors on DangGuiNianTongTang Pasty with MTX on Adjuvant Arthritis in Rats
Yuan Li1, An Liping1
1 The First Affilated Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region , China
Objective: Observing Danggui Niantong Tang pasty’s affection by the adjuvant arthritis (AA), the apoptosis of synoviocytes, serum cytokines and other indicators. Which explore the treatment of rheumatoid arthritis in rats about DangGuiNianTongTang Pasty, with the oral administration of methotrexate(MTX), and compared with MTX +Diclofenac Diethyl amine Emulgel, to explore the early control of disease progression in topical ointments, and a better understanding of DangGuiNianTongTang Pasty and Diclofenac Diethylamine Emulgel’s difference on relevant indicators and symptoms of inflammation improve. Methods: (1) Selected wistar rat 48, according to the random number table they were divided into blank group, model group, treatment group (MTX + Danggui Niantong Decotion of application), and the control group(MTX+ Diclofenac Diethylamine Emulgel), n=12, and number, in addition to the blank group, each rat’s right toe skin injected Freund’s complete adjuvant 0.1ml proinflammatory. (2)Two weeks after the modeling, all rats by intragastric administration of MTX and gauze wrapped drugs and topical administration , administration of a total of 18 days, blank group not be deal with, the model group were given topical poured into distilled water and a simple gauze comfort therapy. (3)Observe the general situation of the rats before and after the modeling, measured by ELISA in IL-17, TNF- , and take the right side of the knee joint cavity within the synovivalɑ tissue, and processed into single cell suspension, add the appropriate reagents, and flow cytometry immediately. Results: (1)The treatment group’s TNF- , IL-17 levels lower than the model groupɑ (P<0.05), the control group’s TNF- , IL-17 levels lower than the model group (P<0.05), the treatmentɑ group’s TNF- , IL-17 level slightly higher than the control group (P<0.05). (2)The treatment group’sɑ synovial cell apoptosis rate was higher than the model group (P<0.05), apoptosis of synovial cells in the control group higher than the model group(P<0.05), synovial cell apoptosis rate of the treatment group
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slightly higher than the control group(P<0.05). Conclusions: (1)The DangGuiNian TongTang Pasty with MTX had good local antinflammatory and analgesic effect, inhibit autoimmune inflammation,restore the abnormal immune regulatory function. (2)The DangGuiNianTongTang Pasty with MTX had a significant effect in improving the rate of apotosis of synovial cells.
A-068Research on the combination of Acupuncture and Auricular Acupuncture treatment for patients with Acute Exacerbation of COPD
Cao Lin1, Liu Zhiyan1
1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region, China
Objective: Observe the influence of life quality and the improvement of pulmonary function with acupuncture combined with auricular acupuncture therapy on patients with acute exacerbation of COPD. Methods: 60 patients with acute exacerbation of COPD were randomly divided into four groups: acupuncture combined with auricular acupuncture group, acupuncture group (acupuncture combined with simulate auricular acupuncture), auricular acupuncture group (simulate acupuncture combined with auricular acupuncture), control group (simulate acupuncture combined simulate auricular acupuncture).Four groups received conventional treatment as basis combined with acupuncture, auricular acupuncture, simulated acupuncture, simulated auricular acupuncture. After two courses (20 days) of treatment, observe FEV1, FEV1/FVC ratio of pulmonary function and CAT, MRC, as well as traditional Chinese medicine symptom scale quality of life scores of the four groups of patients before and after treatment. Results: The lung function: There was statistically significant (P<0.05) of patients with pulmonary function in four groups before and after treatment. acupuncture combined with auricular acupuncture group’s FEV1 、 FEV1/FVC ratio improved compared with before treatment, the improvement of pulmonary function was better than the other three groups; CAT scales reflect the quality of life score decreased significantly compared with before treatment, acupuncture combined with auricular acupuncture group was better than the acupuncture group and auricular acupuncture group, and was statistically significant compared with control group (P<0.05). Conclusion: Acupuncture combined with auricular acupuncture for treating acute exacerbation of COPD patients with the pattern of phlegm obstructed in lung, which is effective in the clinical symptoms, especially the improvement of airflow limitation as well as difficulty in breathing. Four groups compared with each other, suggesting that acupuncture, auricular acupuncture can treat COPD, the combination of their two is better, and patients’ quality of life also improved.
A-069Research on Traditional Chinese Medicine theory and gynaecological diet therapy
Tan Rong1, Liu Ailing1
1 Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China
Objective: This subject is relevant to the diet of traditional Chinese medicine or card literature
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information collection and filing. TCM literature information gynecological dietotherapy comb, and discusses gynecological famous dietotherapy formulae rule, screening by common gynecologic with births suitability dietotherapy method and formulas, for Chinese medicine clinical application and gynecological dietotherapy formulas provide literature development foundation. Methods: the method of literature research system, the han dynasty to Ming and qing dynasties access each time the diet of traditional Chinese medicine (TCM) or certificate of literature material, original literature information identification, collection and management and layout, this study prescriptions altogether collected 761 songs, postpartum disease prescriptions dietotherapy 280 songs, accounting for 36.79% of the total number of prescriptions collection, pregnancy illness prescriptions dietotherapy 276 songs, accounting for 36.27% of the total number of prescriptions collection, menstrual disease diet formula 117 first, accounting for 15.37% of the total number of prescriptions collection, taken down to disease first 45, accounting for 5.91% of the total number of prescriptions collection, miscellaneous diseases and first, accounting for 6.04% of the total number of prescriptions collection. Among them the first 216 han the sui and tang dynasties, accounting for 28.38% of the total number of prescriptions collection; 227 the first jing. Collect accounts for 29.83% of the total number of prescriptions, the Ming and qing dynasties 318 first, accounting for 41.79% of the total number of collection; Postpartum disease of diet in the first 280 prescriptions, han the sui and tang dynasties first 92, accounting for 32.86% of the total number of prescriptions postpartum, 86 the first song, accounting for 30.71% of the total number of prescriptions postpartum disease, the Ming and qing dynasties 102 first, accounting for 36.43% of the total number of prescriptions postpartum disease; The first pregnancy illness 276 prescriptions diet, han the sui and tang dynasties first 87, accounting for 31.52% of the total number of prescriptions pregnancy illness, the song first 83, accounting for 30.07% of the total number of prescriptions pregnancy illness, the Ming and qing dynasties 106 first, accounting for 38.41% of the total number of prescriptions pregnancy illness. Application of frequency analysis, variance analysis methods, combined with traditional Chinese medicine logic reasoning and analogy method, formula metrology research methods, traditional Chinese medicine is the biggest of the limits using drug that diet card, etiology comb and statistical analysis. Results: the gynecology in having to prescriptions diet drug, remove blood stasis drugs, XieBiao medicine, heat the water permeability of drug, wet medicine, hemostatic medicine, the temperature of drug, astringent medicine, medicine this ten kind of drug regulating the accumulative total of frequency up to 92.34%, is the main medicine of traditional Chinese medicine of gynecological diet. Among them, having medicine, remove blood stasis drugs, XieBiao medicine, heat the water permeability of drug, wet medicine, hemostatic medicine, temperature are the most commonly used in medicine, seven of the cumulative frequency of 86.99%, in clinical therapy, plays a main role. Compensation in medicine to fill gas, blood, filling Yang drug is given priority to, accounting for 87.28% of the benefit drug use frequency, four gas temperature, and flat, cold three kinds of the cumulative frequency drugs reached 90.02%, traditional Chinese medicine is a gynecological formula of four gas main diet drugs. Among them the use frequency of chiral drugs temperature highest 43.13%, five in gansu and Michael essien, bitter three kinds of the cumulative frequency drugs reached 89.54%, traditional Chinese medicine is a gynecological formula of five main diet drugs. Among them with our drug use, the highest frequency, 49.97%; Be in the spleen, heart, lungs, liver, kidney, stomach six drugs cumulative frequency to 94.89%, traditional Chinese medicine is a gynecological diet formula of the main to drugs. Among them with the drug use to spleen highest frequency, 21.13%. In all of poisonous drug prescription diet is occupied 9.67%, the drug toxicity is 90.63%. Gynecology in common use in the form of prescription diet is: one, and the wine agent, powder, pills, food, beverage, porridge agent. Respectively 39.68%,
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14.85%, 14.06%, 8.94%, 6.18%, 5.91%, 4.47%. Conclusion: the gynecology diet drug prescriptions range, effect of drug, having to remove blood stasis drugs, XieBiao medicine, heat the water permeability of drug, wet medicine, hemostatic medicine, temperature are the most commonly used in medicine to give priority to. Commonly used drugs have wine, angelica, ginger, born tuberous root, liquorice, root of herbaceous peony, cinnamon, ginseng, astragalus membranaceus, poria cocos, ligustici chuanxiong rhizoma, white donkey-hide gelatine, lamb, honey, largehead atractylodes rhizome, worship singing, big jujube, mugwort, dwarf lilyturf, commonly. General use in gansu and Michael essien, taste bitter primarily, medicinal properties to temperature, flat, cold Lord; Return to the spleen, heart, lungs, liver, kidney, stomach primarily. In one form, and the wine agent, powder, pills, food, beverage, porridge agent mainly.
A-070Distribution characteristics of TCM syndrome and treatment on 2026 Cases of Sub-health Population of Urumqi City Du Shuai1, Hu Xiaoling1*1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China, *Correspondence should be addressed to xjhuxl @yahoo.com.cn
Objective: through the investigation of Urumqi city part of sub health and sub-health population distribution characteristics of TCM syndrome of TCM diagnosis and treatment, clinical program laid the foundation. Methods: by means of questionnaire survey Urumqi city on 2026 cases of sub-health population distribution and the characteristics of TCM syndrome analysis. Results: (1) with sub health diagnosis criteria of 2026 people, including 1052 males,51.92% females,974 people, accounting for 48.08%;1725 Han Chinese people, accounting for 85.14%,301 minority people, accounting for 14.86%; married1533, accounting for 75.66%,371 unmarried people, accounting for 18.31%, divorced/(widowed)122 people, accounting for 6.03%; occupation, teachers, administrative staff, technology and engineering researchers, medical personnel, administrative personnel accounted for 78.61%, workers and business service personnel as well as other accounted for 21.39%; cultural degree with bachelor degree or above more accounted for 57.74%, college education accounted for 42.26% of the following. (2) the distribution of TCM Syndromes of sub health state with Yin deficiency of liver and kidney syndrome of phlegm heat disturbance to maximum, minimum,8card form than the sequence is: liver-kidney Yin deficiency, spleen deficiency syndrome (21.84%) two (16.97%) > liver fire syndrome of Liver-qi Depression Syndrome (14.21%) > (13.72%) > liver stagnation and spleen deficiency card > spleen deficiency and dampness stagnation syndrome (11.50%) > lung spleen qi deficiency syndrome (5.62%) > phlegm heat disturbance syndrome (4.44%). Women with Yin deficiency of liver and kidney syndrome and syndrome of stagnation of Liver-qi see more, men with Yin deficiency of liver and kidney syndrome, heart and spleen deficiency syndrome in two, Han Yin deficiency of liver and kidney, heart lienal two empty; minority with Yin deficiency of liver and kidney, spleen deficiency and dampness, phlegm heat disturbance mainly. Yin deficiency of liver and kidney syndrome is more concentrated in the40~49 years old. Various types of age, gender, ethnic aspects of the sidents in sub-health state has its own characteristics, sex, age, nationality on differences were statistically significant
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(P<0.05). Conclusion: Urumqi city re the sub health are affected, the future of sub health status of Chinese medicine intervention to lay the foundation.
A-071The research on life quality of Yang-yin-huo-wei Formula Treatment of Chronic Atrophic Gastritis Patients
Dai Ming1, Zeng Binfang1*1 Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region ,
China; *Correspondence should be addressed to [email protected]
Objective: To observe the Yang-yin-huo-wei formula treatment of chronic atrophic gastritis on patients report outcomes and quality of life effects. Methods: Select the Xinjiang Uygur Autonomous Region Chinese Medicine Hospital Gastroenterology Clinic in August 2010 to January 2012 207 Patients who was up to diagnostic criteria were randomly divided into treatment group and control group. Were 104 cases of the treatment group and control group (n=103), the treatment group was treated with Yang-yin-huo-wei Formula (one dosage daily, 2 times, morning and evening after meal), while the control group was treated with Weifuchun (3 times daily, 4pills each time). Quantitative score and statistical analysis to compare clinical outcomes and quality of life reported by patients before and after treatment, respectively. Result: (1) 5 overall dimensions of the PRO scale comprehensive comparison of the treatment group points lower than the control group (F=12.824,P=0.000<0.05); Single factor analysis of variance for each dimension, including partial-stomach feeling, the overall body condition, and gastrointestinal symptoms after the three dimensions of the treatment group scores lower than the control group (P<0.05), Integral in two aspects of the psychological state and social health was no significant difference (P>0.05); Scale evaluation of 30 patients reported clinical outcomes after treatment of the state, the treatment group, epigastric pain, epigastric noisy, pantothenic acid, heartburn, dry mouth, 9 entries after treatment, scores were lower than control group (P<0.05); Difference in the scale of 30 patients report clinical outcomes before and after treatment, higher than in the nine entries in the treatment group difference of pantothenic acid, heartburn, dry mouth, epigastric fullness, epigastric pain, epigastric Work for noisy, the control group (P<0.05). (2) Treatment group and control group after treatment, quality of life scores were higher than before treatment (P<0.05), to improve the quality of life in patients. Two groups of patients to treatment group in addition to treatment in this dimension of the emotional functions (RE), higher than that of the control group (t=-2.33, P=0.021); in the other seven dimensions with the control group no significant difference (P>0.05). The difference between the two groups of quality of life in addition to the dimension of the mental health (MH) difference was statistically significant (t= 2.45,P= 0.015 <0.05), and the treatment group was higher, while in the other seven-dimensional degree has had no statistically significant (P<0.05). Conclusion: (1)Yang-yin-huo-wei formula a good therapeutic effect of atrophic gastritis, improve the clinical outcomes of patients reported overall better than Weifuchun, mainly reflected in the local epigastric feeling, body and the overall state of gastrointestinal symptoms in three aspects. Among them, the nine entries of epigastric pain, epigastric noisy, pantothenic acid, heartburn, dry mouth, such as therapeutic effect obvious while in epigastric fullness, epigastric pain, epigastric noisy, pantothenic acid, stomach burning sensation, dry mouth, and other nine entries in the degree of improvement is obvious. (2) Yang-yin-huo-wei formula and Weifuchun can improve the quality of life of patients, while
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no significant difference between in the two (P>0.05).
A-072Anti-hypertensive effects of the aqueous extracts of Chinese yam (Dioscorea opposita Thunb.）in Two-Kidney, One-Clip Renal Hypertension Rat model
Nurmuhammat Amat1, Palidan Wushuer2, Sajida Abdureyim3, Dang Ming4, Dolkun Mamut 5*, Anake Kijjoa 6
1Traditional Uighur Medicine Institute, Xinjiang Medical University, 830011, Urumqi, Xinjiang, PR China; 2 Psychosomatic
Department of Traditional Chinese Medicine Hospital, Xinjiang Medical University, 830011，Urumqi, Xinjiang, PR China; 3First
Affiliated Hospital, Xinjiang Medical University, 830011，Urumqi, Xinjiang, PR China; 4Postgraduate College, Xinjiang Medical
University, 830011， Urumqi, Xinjiang, PR China; 5Salamat Biotechnology Co., Ltd. Xinjiang, China; 6ICBAS - Instituto de
Ciências Biomédicas de Abel Salazar and CIMAR, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313
Porto, Portugal.*Correspondent to [email protected] or Dr Nurmuhammat Amat, [email protected].
Objective: Chinese yam (Dioscorea opposita Thunb.) is a medicinal herb used in traditional Chinese medicine to treat different system diseases including hypertension. The objective of the study was to investigate the possible antihypertensive effects of Chinese yam (CY) extract in renovascular hypertensive rats as well as the mechanism in reducing blood pressure. Methods: The two-kidney one-clip (2K1C) Goldblatt model of renovascular hypertension was used in Wistar rats. Rats were randomized into sham, untreated 2K1C, captopril-(30mgkg−1 per day orally) and CY-(210, 420mgkg−1 per day orally)，medium (M)-CY, high (H)-CY treated 2K1C groups (n=10-12 per group), followed up for 6 weeks. The Blood pressure, cardiac mass index (heart weight/body weight), plasma level of angiotensin-II (Ang-Ⅱ), endothelin-1(ET-1), superoxide dismutase (SOD) and malondialchehyche (MDA) were evaluated. Results: CY significantly reduced mean systolic and diastolic blood pressure after treatment (P<0.05). CY also significantly increased plasma SOD activity, decreased plasma MD concentration. Renal function was improved with captopril and CY. Ang-Ⅱwas reduced by CY to same extent with captopril. ET was reduced to lower concentrations (74.79 ± 17.88, 90.75 ± 39.31pgml−1 for L-, H-CY, respectively) than in sham (116.12 ± 39.34pgml−1), untreated 2K1C (170.19 ± 39.34pgml−1) or captopril (68.22 ± 22.10pgml−1) groups. They also significantly reduced the left ventricular hypertrophy and cardiac mass index. These findings are in agreement with the traditional use of CY in the treatment of arterial hypertension. Conclusion: Our results also suggest that CY may have an antihypertensive effects on hypertension by inhibit ET-converting enzyme and antioxidant activity, and warrant further exploration.
A-073Effect of TongFeiJingTanFang on Immunologic Function of Phlegm Heart Syndrom in Chronic Obstructive Pulmonary Disease
Bai Zhengping1, Tan Guangbo1, Hu Xuejun1
1Affiliated Hospital Combining Traditional Chinese & Western Medicine of Hunan University of TCM, Changsha, China
Objective: To observe the clinical effects and immunologic function of TongFeiJingTanFang in treating phlegnr heart syndrome of chronic obstructive pulmonary disease (COPD). Methods: 114 subjects
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were randomized into two groups：58 cases were treated by not only conventional therapy but also TongFeiJingTanFang (experimental group), and the remaining 56 cases were subjected to conventional therapy (control group). The changes of the respiratory symptome , the scores of patients’life quality including body mass index, St . George respiratory questionnaire, pulmonary function test, and 6-minute walking distance, and levels of plasma immunologic function were compared between two groups. The course was four weeks. Results: Total effective rate in the experimental group was better than that in the control group (P<0.05). There was significant statistical differences between the two groups, which shows that the experimental group was superior to the control group in amelioration of syndrome including cough and sputum ,and the integration of SGRQ, increasing levels of CD4+、CD4+/CD8+、 IL-2,decreasing CD8+、 IL-8. Conclusion: TongFeiJingTanFang can allevate respiratory symptom, improve quality of life and regulate immunologic function in patients who are phlegm heart syndrome of COPD.
A-074Effects of “Yang-Warming and Kidney Essence-Replenishing Herbal Paste” on Cold-related Asthma Exacerbation——A Report of 74 Cases
Tang Binqing1*, Shi Kehua1, Li Xiaoqi1, Wang Hongchang1, Fang Hong1, Xiong Bidan1, Wu Yingen1 1Shanghai University of Traditional Chinese Medicine , Longhua Hospital, No. 725 Wan Ping South Road , Shanghai, 200032,
China; *Correspondence should be addressed to [email protected]
Objective: To observe the effects of “Yang-Warming and Kidney Essence-Replenishing” herbal paste on cold-related asthma exacerbation. Methods: A total of 151 cases of moderate to severe persistent asthma (3 cases didn’t finish the expected treatment and 5 cases lost during follow-up) were randomly divided into a herbal paste treatment group (74 cases) and a control group (69 cases). Both groups were given basic treatment according to Global Initiative for Asthma (GINA) scheme and the treatment group was also treated with herbal paste in winter. The onset frequency of asthma exacerbation,cold and cold-related asthma exacerbation, ACT score and kidney-deficiency syndrome score within one year in each group were observed. Results: Compared with control group, the onset frequency of asthma exacerbation, cold and cold-related asthma exacerbation, and kidney-deficiency syndrome score were significantly reduced（P<0.01）. There was no significant difference in ACT score between the two groups. Conclusions: Receiving treatment of “Yang-Warming and Kidney Essence-Replenishing” herbal paste in winter can reduce cold-related exacerbation of asthma.
A-075Huang Di Nei Jing health theory and the prevention and treatment for chronic obstructive pulmonary disease
Xu Yanling1
1Department of Respirology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Beiling Dajie No. 33,
Huanggu District, Shenyang 110032, Liaoning Province, China
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Huang Di Nei Jing is one of the earliest books in the existing medical literature in China. It gives a comprehensive overview of the system architecture of the theoretical system of Traditional Chinese medicine (TCM), reflects the theoretical principles and academic thought of TCM, and it also laid the foundation for the development of TCM. Huang Di Nei Jing is not only a great medical masterpiece, but also a great book for health science and rehabilitation science. The “Preventive Treatment of Diseases”idea it advocated and its wealth of health theory and experience are still of great significance for the guidance of disease prevention and cure. In this study, we investigate the role of Huang Di Nei Jing health theory in guiding the prevention and treatment of chronic obstructive pulmonary disease (COPD) from harmony with nature, living a regular life with certain rules, regulating emotion, keeping a clear mind; eating and drinking abstemiously; drugs and food simultaneously; sex abstemiously, keeping yin fluid, focusing on prevention, preventive treatment of diseases. We emphasis that the daily lives of COPD patients should follow the nature of yin and yang fluctuation law, should work and rest appropriately that can not only reduce the number of acute attack, and also can alleviate the acute exacerbation. We attach importance to the human spirit emotions and activities, balancing diet and sex abstemiously in the prevention and treatment of COPD. Also, we advocate early prevention, early diagnosis and early treatment of COPD. Finally, we concluded that using the Huang Di Nei Jing health theory to guide clinical practice will bring to the overall improvement of the prevention and treatment of COPD and other chronic diseases.
A-076Influence of Compound Panax notoginsenosideGranules (CPNG) on bleomycin- inducedpulmonary fibrosis in rats
Ling Chunhua1, Wu Juanjuan2
1First Affiliated Hospital of Suzhou University, 215006, Jiangsu Suzhou, China; 2Suzhou Chinese Medicine Hospital, 215006,
Jiangsu Suzhou, China
Objective: To explore the effects of Compound Panax notoginsenoside Granules (CPNG) on bleomycin-induced pulmonary fibrosis in rats and its mechanisms. Methods: Sixty male Sprague-Dawley rats were randomly divided into 6 groups, including control normal group，model group, prednisolone group(3.33mg·kg-1·d-1) and CPNG high-dose, moderate-dose and low-dose group (100、50、25mg·kg-1·d-1), 10 Sprague-Dawley rats in each group. Except the control normal group, pulmonary fibrosis models were reproduced by intratracheal injection of bleomycin(5mg/kg). On the second day of injection of bleomycin, the rats in each treatment group were intragastric administrated prednisolone and corresponding dose of CPNG daily; control normal group and model group were intragastric administrated the equal volume distilled water daily. After 28 days, all rats were executed to be observed the pulmonary index. Hydroxyproline (HYP) in lung tissue was assessed. Fixed parts of the lung tissue was obtained for HE and Masson stain for histopathological examination. Malondialdehyde (MDA), glutathion (GSH), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in blood and lung tissue were respectively assessed by means of Thibabituric Acid(TBA), chemical colorimetry, xanthine oxidase and chemical colorimetry. The expressions of transforming growth factor β1 (TGF-β1), α-smooth muscle actin (α-SMA), Smad2, Smad3 and collagen I (Col-I) protein were analyzed by immunohistochemistry. The expressions of TGF-β1, Smad2, Smad7 protein were analyzed by western-blotting. Results: Compared with model group, the pulmonary index and lung tissue HYP were
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decreased (P<0.05, P<0.01), MDA level in blood and lung tissue was also decreased(P<0.001), but GSH, SOD, T-AOC level in blood and lung tissue were significantly increased (P<0.05, P<0.01, P<0.001) in all of CPNG groups . Histological evaluation of lung specimens demonstrated that CPNG dramatically decreased the lung inflammation and lung fibrosis in rats(P<0.01 ， P<0.01). Munohistochemistry and western-blotting demonstrated that CPNG could inhibite the expressions of TGF-β1, α-SMA, Smad2, Smad3, Col-I protein(P<0.05, P<0.01, P<0.01), and increase the expression of Smad7 protein in lung tissue of rats(P<0.01). Conclusion: CPNG has a therapeutic effect on bleomycin-induced pulmonary fibrosis in rats, which maybe relate to antioxidative damage, intervention TGF-β1/Smads signaling pathway, and restraining the formation and deposition of collagen.
A-077Qufeng Xuanfei side clinical study of treatment of allergic cough
Xu Lihua1, Li Lei1, Huang Ye1
1Jiangsu Provincial Chinese Medicine Hospital, 21002, Nanjing, China; 2Liyang City Hospital of Chinese Medicine Hospital,
213300, Liyang, China
Objective: To observe the clinical curative effect of the traditional Chinese medicine on atopic cough. Methods: 60 cases with atopic cough patients were randomly divided into two groups. 30cases of the treatment group, treated by the Chinese traditional curative methed. (medicine: sangye、qianhu、baibu、chanyi、zhijiangcan、pingdimu、zhimahuan、pipaye、gaicao. Increase or deduce the quantity of different medcine according to different causes of desease, eg: Wind and cold attacking the lung syndrome, add fangfeng、suye、guizhi、jiegeng; Wind and dryness attacking the lung syndrome add maidong、 yuzhu、 nanshasen、 zhimu; Wind and heat attacking the lung syndrome add yinhua、sangbaipi、lugen、huangqin、xingren；a decoction per day).30 cases of the treatment group with the treatment of loratadine, we need the observe the syndrome score and main symptom index of the patients. Results: The total effective rate of treatment group is 86.67%, control group in the total efficiency is 76.67% . The difference was statistically significant. The symptom scores were improved in both groups, while the result of the treatment group is more obvious than that control group, with statistical significance. Conclusion: Atopic cough syndrome belongs to Chinese traditional medicine, " feng Ke " type, self-made “Qufeng Zhike” treatment can significantly improve symptoms of those who have atopic cough and reduce the allergic level of the patients. Chinese traditional treatment has certain advantages and is worthy of further study and researed.
A-078Randomized Control Trial of JinhuaQinggan Granule in Treating Influenza with Wind-Heat Affecting Lung Symptom-Complex
Li Guoqin1, Liu Zhiguo1, Liu Qingquan1
1Guang’anmen Hospital affiliated to China Academy of Chinese Medical Sciences, 100053, Beijing, China
Objective: To observe the effect and safety of JinhuaQinggan Granule (JHG) in treating influenza with wind-heat affecting lung symptom-complex (WHALS), besides study the optimal dosage of it. Methods: Influenza patients with WHALS were enrolled from 6 clinical center of the study. They were randomized
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divided into 3 group, high dosage group, low dosage group and the placebo group, then they receive 5 days medication before valuation. Results: 136 cases confirmed with influenza were enrolled from 992 patients, statistical analysis shows low dosage group of JHG significant reduced the estimated median time to fever resolution compared with the placebo group, synthesize Chinese medical curative effect in low dosage group is better that of placebo group at the 3th and 5th day from treatment. High dosage group has a similar effect with low dosage group except inferior to partial criteria. There is no significative difference among these three groups. Conclusion: JHG is effective and safety in treating influenza with WHALS, Low dosage is the optimal dosage of JHG.
A-079Scorpion - centipede on the rat model of asthma airway inflammation and airway remodeling in experimental research
Li Haiyan, Yang Peilan, Huang Haiyin, Cui Jingang, Tang Jie, Wu Yingen
Objective: Observe the impact of “scorpion-centipede” on airway inflammation and airway remodeling of asthmatic rats, to explore the mechanism of action of “scorpion-centipede” for asthma. Methods: Thirty SD rats were randomly divided into three groups:normal control group,model control group, Chinese medicine treatment group,10 in each group.The asthmatic rat airway remodeling model was established by ovalbumin sensitized method, “scorpion-centipede” was applied to intervene. Preparation of bronchoalveolar lavage fluid(BALF) and lung tissue biopsy harvested after three weeks of treatment. The HE staining of the airway structure changes, and Masson staining of smooth muscle, collagen fibres, and determination of the extent of the airway remodeling in medical image analysis software. Results: Compared with normal group, number of total leukocytes and the percentage of eosinophil, neutrophil, lymphocyte in BALF were significantly higher in the model group(P<0.01),the thicker of Bronchial wall, smooth muscle layer and collagen fibres was significantly increased in the model group(P<0.01); Compared with model group, number of total leukocytes and the percentage of eosinophil, neutrophil, lymphocyte in BALF were significantly lower in the Chinese medicine treatment group (P<0.01),the thicker of Bronchial wall, smooth muscle layer and collagen fibres was significantly decreased in the Chinese medicine treatment group (P<0.01). Conclusions: Scorpion-centipede reduce airway inflammation of asthmatic rats, plays an inhibitory role in airway remodeling by reduce the bronchial wall and smooth muscle thickness, reduce the proliferation of collagen fibres.
A-080The application of Lung and Large intestine Interacts relations in COPD
Geng Limei1 1 Hebei Province Chinese Medicine Hospital, 050011, Shijiazhuang, China
Objective: To investigate the application and mechanism of Lung and Large intestine Interacts relations in COPD. Methods: Objective 32 AECOPD patients with retention of phlegm heat syndrome in the lung. The control group was treated with routine method. In the treatment group, we added Qingre Xiefu Chinese traditional medicine. Result: Total effective rate of the Control group was 93.75 % and the treatment group was 87.50%. There were obvious differences between the control group and the
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treatment group (P<0.05). Conclusion: Qingre Xiefu Chinese traditional medicine was helpful to the AECOPD patients with retention of phlegm heat syndrome in the lung. Lung and Large Intestine theory of clinical value in COPD was worthy of promotion.
A-081The effect of Er Chen Jie Han Particle for the treatment of OSAHS patients with Phlegm Damp Syndrome
Wang Zhen1, Yang Junchao1, Shen Yingying1, Xu Tingzhen1
Objective: To appraise the effect of Er Chen Jie Han Particle for treatment of OSAHS patients with phlegm damp syndrome. Methods: 80 patients of the outpatient and ward ， in the form of envelopes，were randomly divided into two groups．Control group，40 cases，were treated with a healthy lifestyle for 3 months．Treatment group，40 cases，were treated with Er Chen Jie Han Particle on the basis of a healthy lifestyle for 3 months ． Before and after the end of the treatment ， patients were observed blood lipids 、 body mass index （ BMI ） 、 blood viscosity、polysomnography detection（PSG）、Epworth sleepiness scale、Quality of life. Results: 1.Compared with before treatment ， BMI of control group had obvious improvement (P<0.05), TG、CHOL and BMI of TCM group had obvious improvement (P<0.05)；2.Compared with before treatment， low shear of whole blood viscosity of control group had obvious improvement (P<0.01), both low and middle shear of whole blood viscosity of TCM group had obvious improvement (P<0.01). After treatment, compared with the control group， low shear of whole blood viscosity of TCM group showed significant difference (P<0.01)；3.Compared with before treatment，both the lowest oxygen saturation and Quality of life of control group had obvious improvement (P<0.01 or P<0.05)，AHI、the lowest oxygen saturation、Epworth sleepiness scale and Quality of life of TCM group had obvious improvement(P<0.01 or P<0.05. After treatment, compared with the control group，the lowest oxygen saturation、Epworth sleepiness scale and Quality of life of TCM groups showed significant difference (P<0.01). Conclusion: On the basis of healthy living，Er Chen Jie Han Particle can lower BMI、blood lipids and blood viscosity ， significantly improve low ventilation and hypoxia ， reduce daytime sleepiness symptoms，improve quality of life of OSAHS patients with phlegm damp syndrome．
A-082The search of basic law of the ancient physicians using the prescription medication to treat "Pulmonary heart disease in the remission stage"
Hu Xuejun1, Bai Zhengping1*, Tan Guangbo1, Gan Rong 1, Liu Min 1
1Affiliated Hospital of Hunan Province Institute of Traditional Chinese Medicine, Changsha 410006, China; *Corresponding
authors: Affiliated Hospital of Hunan Province Institute of Traditional Chinese Medicine, Hunan Yuelu District, Foothills No. 58,
tel+86-13873100306，tel +86-0731-88904052, 410006, China
Objective: To understand the basic law of the ancient physicians using the prescription of medication to treat “Pulmonary heart disease in the remission stage ”. Methods: Representative from the 30 remnants of Chinese ancient books, to collect the prescriptions, Chinese material medica, the dosage and the amount of content that the ancient physicians using to treat “Pulmonary heart disease in the
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remission stage”. And the content were classificated, statisticed, analyzed and summarized. Results: Sorting out the ancient prescriptions 110, involving more than 10 types of medicines, the main treatment method of 14 kinds. Tonifying drugs accounted for 38.2% totally. Drugs of reducing phlegm, relieving cough and asthma accounted for 13.3% totally, the second is the diaphoretic, the heat-clearing drugs, damp-clearing drugs,qi-regulating drugs,astringent drugs, interior-warming drugs, blood-activating drugs, aromatic damp-resolving drugs, etc. Conclusion: when the ancients cure this disease, they often tonificate and purgate in combination, taking tonification as the main, tune up the lung, spleen and kidney together, to tonify the qi is the first, and pay more attention to reduce phlegm, to relieve cough and asthma.they took the ways of clearing the surface, clearing the heat, regulating qi, invigorating the blood, inducing astringency, warming the inner body, clearing the water and clearing dampness accordingly. At the same time, the antients took the drug compatibility , two-ways adjustment etc seriously.
A-083
Rharmacodynamic Research on Liver Protecting, Choleretic and Immune Regulating Function of Deduhonghua-7 Powder
BaTudeligen1，Wu Enbaoyin1 ，Na Risu1，Han Zhiqiang1
1Institute of Clinical Pharmacology of Traditional Mongolian Medicine, Affiliated Hospital of Inner Mongolia University for the
Nationalities, Tongliao, 028007, China
According animal experiment, observe the protecting function, choleretic and immune regulating function of Deduhonghua-7 powder on D-GaLN acute liver injury.Method: The Mongolian Medicine Deduhonghua-7 powder is experimented drug of this research, set high dose group (0.830.83 g•kg-1)and low dose group(0.5 g•kg-1). All experimented groups were given drug continues by intragastric administration for one week. Made mice acute liver cell necrosis model by intraperitoneal injection of D-GaLN to observe morphology changes of liver, to evaluate the chemical protecting function of Deduhonghua-7 powder objectively; observe the mouse peritoneal macrophages phagocytosis of chicken erythrocytes, evaluate the influences of Deduhonghua-7 powder at phagocytosis function of morphology; use the gallbladder removing and weighting method to observe the influence of Deduhonghua-7 powder at secretion of bile; use MTT method to observe the influences of Deduhonghua-7 powder at mouse spleen, lymph cell proliferation induced by concanavalin A.Result: At acute liver injury experiment, comparing with normal group, the acute liver injury group’s ALT, AST of mice serum were increased(P<0.05), comparing with modeling group, all Deduhonghua-7 powder group and Bifendate group were decreased (P<0.05), comparing with modeling group, Deduhonghua-7 powder group and Bifendate group’s tissue pathological changes obviously decreased. At macrophage phagocytosis experiment, compared with control group, all treating groups’ phagocytosis rate and phagocytosis indexes had significant differences(P<0.05). At choleretic experiment, comparing with control group, the all Dedhuhonghua-7 powder group’s secreting of bile were increased, had significant
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differences (P<0.05). At mice spleen, lymph cell proliferation experiment, comparing with control group, the OD value of Deduhonghua-7 powder group were increased, had significant differences (P<0.05). Deduhonghua-7 powder has hepatoprotective and enzyme decreasing function at mice acute liver injury induced by D-GaLN; Can increase mice bile secretion significantly; Can significantly increase the abdominal macrophage phagocytosis function; Can improve the proliferation of lymph cell induced by ConA.
A-084The Clinical Study Between Syndrome Type in TCM and the Serum Levels of IL-25 and Total IgE in Patients with Bronchial Asthma
Liu Zongwei1, Zhou Xianmei 2
1Department of Pulmonary Medicine, Lianyungang City Hospital of Traditional Chinese Medicine, Changshu 222004, Jiangsu,
China；2Department of Pulmonary Medicine, Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing 210029, Jiangsu, China
Bronchial asthma is one of the common and frequently occurring diseases in respiratory system. In traditional Chinese medicine (TCM), the types of bronchial asthma are diagnosed via four examination techniques including inspection, auscultation-olfaction, inquiry, and palpation. Therefore, the aim of the current research is to study the correlation between serum interleukin 25 (IL-25), total immunoglobulin E (IgE) and common clinical bronchial asthma with cold syndrome, bronchial asthma with heat syndrome, and deficiency syndrome of both lung and kidney, explore the material basis of TCM diagnosis of the syndromes, and provide scientific evidences for the diagnosis of the syndromes of TCM. 60 patients with bronchial asthma and 20 healthy volunteers were involved in the current study. The patients were devided into 3 groups according TCM theory, cold syndrome, heat syndrome, and deficiency of both lung and kidney groups, each group consisits of 20 subjects. Blood was collected from each subject and serum (2 ml) was separated and stored at -80 oC until IL-25 and total IgE were measured. The IL-25 level was detected by enzyme-linked immunosorbent assay (ELISA) method and the total IgE was determined by Chemiluminescence Method. The level of serum IL-25 of bronchial asthmatic groups were significantly higher than that of healthy control group (32.54 ± 9.12, P<0.01). Although the level of serum IL-25 in bronchial asthma with cold syndrome type was not statistically significant compared with the heat syndrome type (P>0.05), the IL-25 level in bronchial asthma with cold syndrome type (59.16 ± 12.94) and heat syndrome type (63.74 ± 13.74) were significantly higher than those in the deficiency syndrome type of both lung and kidney (44.09 ± 8.67, P<0.01). The level of serum total IgE of bronchial
asthmatic groups were significantly higher than that of healthy control group（38.31 ± 26.71, P<0.01).
The level of serum total IgE in bronchial asthma with heat syndrome type （439.12 ± 279.44）was
significantly higher than that in the cold syndrome type (256.34 ± 100.20, P<0.01). The total IgE level in cold syndrome type was markedly higher than that in the deficiency syndrome type (122.53 ± 44.83, P<0.01). The level of serum total IgE in bronchial asthma with heat syndrome type was significantly higher than that in the deficiency syndrome type of both lung and kidney (P<0.01). 3. In asthmatic
groups, serum level of total IgE was positively correlated with that of IL-25 （ r=0.354，P<0.006） .
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Some correlation was observed between serum IL-25 and total IgE levels and bronchial asthma with TCM diagnosis of the syndromes. Serum IL-25, total IgE levels could be used as a reference for the diagnosis of TCM types of the asthma.
A-085Clinical Observation on Chronic Obstructive Pulmonary Disease of Acute Aggravating Stage Treated with Compound Fuercao mixture
Shi Kehua1, Zhe Zhe1, Lv Mimi1, Xiong Bidan1, Zhao Zhi xiang1
1City affiliated hospital of Shang Hai Traditional Chinese Medicine University, Shanghai 200071
To investigate the clinical efficacy of Compound fuercao mixture on acute exacerbation of Chronic obstructive pulmonary disease and the impacts on Serum inflammation factors. 60 acute exacerbation of COPD patients were chosen and divided into 2 groups randomly, 29 cases in treatment group and 31 patients in control group. All patients accepted Conventional western medicine therapy; besides, the treatment group patients took Compound fuercao mixture the same time. The treatment course for both of the treatment group and the control group were 14 days. The total effectiveness of the Compound fuercao mixture group is 92.59%, while the comparison group is 78.57%, there is a statistics difference (P < 0.05)between the two group on the total effectiveness; And the treatment group ,the levels of serum IL-8 decreased obviously. Compared with the control group,also have a statistics difference (P < 0.05);and the treatment group, IL-2 levels were increased after the treatment, there is a statistics difference(P<0.05)compared with the control group; There is no differences on the decrease of the levels of serum IFN-γ (P>0.05). Exacerbation of COPD patients, using Compound fuercao mixture and cooperating with Conventional western medicine therapy acute , can better improve patients with cough, cough up phlegm, breathing symptoms, improve the clinical effect. Its mechanism may be related to reduce the levels of IL- 8, increase the content of IL-2, and may play the role by reducing the inflammatory reaction of the airway. It may also take effect through the adjustment of immune function.
A-086Effective cases analysis of Professor Wu Yin-gen in differentiating and treating interstitial lung disease
Shi Kehua, Xiong Bidan, Yu Xiao, Wu Yingen2
1Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,
200071, Shanghai, China; 2Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, 200032,
Shanghai, China
This article introduced the clinical experience of Professor Wu Yin-gen who differentiated and treated interstitial lung disease by analyzing effective cases. The important point of syndrome differentiation and treatment is that lung collaterals is the position of the disease, obstructing of lung collaterals and intermingled phlegm and blood stasis are the basis pathogenesis of interstitial lung disease. Deficiency, phlegm and blood stasis are the main pathological features. And tonifying the lung collaterals is the method of treatment. Professor Wu thinks that collaterals deficiency must be tonified, and the disease
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can be treated by using therapeutic principles and methods like reducing phlegm and vein relaxing, promoting blood circulation for removing blood stasis, warming yang and benefiting qi, replenishing vital essence to tonify the kidney,and so on. Professor Wu makes full use of drastic,toxic and insects medicine of TCM.
A-087Experimental Studying of Type 2 Diabetes Animal Models through Decoction of baihuginseng-chickpea mixture
Ji Yan1, Wang Cunfen1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: By the statistical analysis through experimental observation and laboratory testing index to evaluate the hypoglycemic effect of the baihuginseng-chickpea mixture in type 2 diabetes animal models. To indicate the hypoglycemic effect by the chickpea original potions decoction. It is used to provide theoretical basis for reasonable development. Confirming the clinical guidance function of heat treatment of Zhang zhongjing nourish "Yin" XiaoKeBing theory. Methods: Selected 90 healthy SD male rats, which were randomly divided into blank control group, model group, baihuginseng group, chickpea decoction group and baihuginseng-chickpea mixture group, using a high-fat diet breeding joint injection STZ (45mg/kg) to establish type 2 diabetes animal models. Then with baihuginseng soup, chickpea decoction and baihuginseng-chickpea mixture to intervene in three weeks to observe the changes of rats blood glucose and the pathological changes of rat pancreas and liver. Results: (1) one week after treatment, the increase of glucose level belonging to the three treatment groups was inferior to model group, but this was not statistically significant. (2) two weeks after treatment, the increase of blood glucose of baihuginseng group, chickpea decoction group was inferior to the model group, but this was not statistically significant. Compared with the model group, the blood glucose of mixture group had the value and statistical differences (P<0.05). (3) three weeks after treatment, compared with model group, the increase of the blood glucose of the medicine-consumption groups were differences (P<0.05), the blood glucose of baihuginsen group and the chickpea decoction group declined lower larger; the mixture group compared to the remaining two treatment groups declined obviously (P<0.05), but compared with normal control group there still had partial gap (P<0.05). Conclusion: (1)Baihuginseng-chickpea mixture has the effect that reduce blood glucose.(2) The chickpea with Baihuginseng soup in reducing blood glucose has synergistic effect.(3) The chickpea original potions decoction has the effect to reduce blood glucose .
A-088Preliminary study on Preparation Technology, Quality Standard and Pharmacodynamic for Nigella glandulifera Freyn Particle
Xiao Qiong1, Jiang Lin1, Li Xiaojin1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
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Objective: (1) To establish the preparation procedure of Nigella glandulifera Freyn Particle; (2) To establish the quality standard of Nigella glandulifera Freyn Particle. (3) Preliminary study on Pharmacodynamic for Nigella glandulifera Freyn. Methods: (1) Extraction procedure: Use routine and ethanol extraction with the synthetic score as index to select the optimum ethanol extraction process by single factor design and orthogonaldesign method. (2) Formation procedure: The significance of multiple index analysis in the formation process. (3) Quality standard: the quality standard of particle was identified by TLC and HPLC. (4) Pharmacodynamics: Use the animal model of Kidney YangDeficiency, An elementary research for it. Use the maximum tolerated dose shaw it can be used safely. Result: preparation procedure: add 16 times of 60% ethanol, reflux-extracting for 2 times 1 hours each time, get the solid extract by drying; adding 10% LHPC, the pellet were made by 93% alcohol， adding 0.5% of stearil acid-Mg to press particle. Angelica in particle made qualitative differentiation with TCL, the content of rutin was understanded with HPLC. Pharmacodynamic: It was assayed that growth of sex organs, weight-bearing swimming time of the experiment. the effects of Nigella glandulifera Freyn on mice with kidney-yang deficiency. Acute toxicity test in mice Nigella glandulifera Freyn are not observed in animals poisoned or mortality. Conclusions: During all the digester modeling: technique and nitial stability study，acute toxicity test is reliable， the Process is controled for quality and noxious Property that will do well data for new drug to report to a higher authority. Complex prescription decoction it can invigorate the kidney. Acute toxicity test can be judged by its toxicity and has little or no toxicity for the further development and application of a theoretical basis.
A-089Preliminary Study on the Chemical Composition of Nigella glandulifera Preyn
Xu Jingjing1, Xue Jie1, Jia Xiaoguang1
1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: Study the chemical composition of Nigella glandulifera Preyn,establish the certain scientific basis for future study. Methods: 1) This article study the N. glandulifera Preyn, extracted and separated it, Determining Structures with chemical and spectral methods. 2) Determination of rutin in Nigella glandulifera Freyn by HPLC. 3) extrated total alkaloid of this plant,Using Box-Behnke central composite design and Minitab analyse the results. Results:1) the materia medica was extracted with chloroform, It has separated 11 componds from extraction and determined 5 compound.(Ⅰ),3-O-[α-L-rhamnopyranosyl-(1-2)-α-L-arabinpyanosyl]-hederagenin(Ⅱ),3-O-[β-D-xylopyranosyl-(1-3)-α-L-rhamnopyranosyl-(1-2)-α-L-arabinpyanosyl]-hederagenin(Ⅲ),3-O-[α-L-rhamnopyranosyl-(1→2)-α-L-arabinpyranosyl-28-O-[α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl]hederagenin(Ⅳ)and3-O-[β-D-glucopyranosyl-(1→6)-α-L-rhamnopyranosyl-(1→2)-α-L-arabinpyranosyl ]-28-O-[α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranosyl-(1→6)-β-D-glucopyranosyl]hederagenin(Ⅴ). 2) rutin was linear over the range of 0.02μg -0.1μg (r=0.9997).The average recovery was 95.3％,with an RSD of 1.4％. 3) the optimun conditions were that EtOH density
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of 69%,the ratio of bittermelon to solvent of 10 mL·g-1,extracting temperature of 83℃ and extracting time of 160min. Under such these conditions the extraction rate of total alkaloid could be up to 3.89 mg·g-1 at one-time extraction. Conclusion: extraction solvent and methods is appropriate of N. glandulifera Preyn studying，it has identified 5 compounds by silica gel, glucan methods; The method of determinating rutin by HPLC and extracting total alkaloid from this plant is convenient, accurate and reproducible.
A-090Kidney’s Concept of TCM and Mechanism, Method, Prescription, Drug & Application of Kidney-invigorating
Wang Genfa1,2, Dong Jingcheng1*, Duan Xiaohong1,2
1Department of Integrated Traditional Chinese and Western Medicine of Huashan Hospital Affiliated Fudan University,
ShangHai, China; 2Institute of Integrated Traditional Chinese and Western Medicine of Fudan University, Shanghai, China;
*Corresponding author: Tel.: +86 21 52888301, Fax: +86 21 52888265, E-mail address：[email protected]
Kidney of TCM is very rich in connotation, which is thought as congenital nature and closely linked with human birth, aging, disease, death and reproduction. Kidney plays a key role throughout life activities. This paper described concept of kidney in TCM and mechanism, method, prescription, drug & application of invigorating kidney, to improve our understanding for the kidney & the thought of treating different diseases with the same method by invigorating kidney, and improve the research of kidney essence on a new level in future.
A-091Is “abnormal black bile” merely imaginary philosophical concept that has no medical meaning?
Halmurat Upur1, HR Tang2, Batur Mamtimin 1, Peierdun Mijiti 1*, Aynur Matsidik 1, Nurmuhammat Amat 1, Wang Duolao 3, Moore Nicholas4, Begaud Bernard4,Igor Gogol 5, Denis Dubrovin5
1 Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi, 830011, China; 2State Key Laboratory of Magnetic
Resonance and Molecular and Atomic Physics of Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences,
Wu Han, 430071, China; 3Department of Medical Statistics, London School of Hygiene and Tropical Medicine, London
WC1E 7HT, UK; 4Department of Pharmacology, University Victor -Segalen Bordeaux 2, 33076 Bordeaux, France. 5Department
of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia; *Correspondence to: Peierdun Mijiti
The humoral theory, as a fundamental theory of Hippocratic medicine, has explained disease, psychology, habit and personality for more than 1500 years. Essentially, this theory holds that the human body was filled with four basic substances called humors-blood, phlegm, yellow bile and black bile, and that a natural body function depends on normal coexistence of four humors and an excess or deficit of one of these four humors would cause disease and comprise what is termed “abnormal humors” (1). From Hippocrates onward, the humoral theory was adopted by Greek, Roman and Islamic
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physicians, and became the most commonly held view of the human body among European physicians until the advent of modern medical research in the nineteenth century. From humorlism’s points of view, Cancers was described by two successors of Hippocratic medicine- Galen and Avicenna as a disease caused by an excess of “black bile” (literally “savda” in Arabic) in body which would lead to “abnormal black bile ” (2,3). Avicenna did not only provide diagnostic criteria for “abnormal black bile” but also gave tips on treatment of cancers which were described in detail in his book-Cannon of Medicine (3). Although four humors theory has been discarded since 19th century, it is still practiced in some parts of the world such as Xinjiang Uygur region of China, India, and Iran etc., and some researches even indicate patients with “abnormal black bile” were significantly different from those with “non-abnormal black bile” (abnormal blood, yellow bile and phlegm) in terms of gene polymorphism, pre-thrombotic state and oxidative stress etc. (4-7). However, is “abnormal black bile” an existing phenomenon with distinct biological basis or merely imaginary philosophical concept that has no medical meaning and hindered the development of medical medicine for more than 1500 years? We undertook this investigation to answer this question. In humoral theory, health depends on balance of four humors (blood, phlegm, yellow bile and black bile) in body and an excess or deficit of any one of these humors would cause disease and comprise what is termed “abnormal humors”. Cancer was thought to be caused by an excess of “black bile” which lead to “abnormal black bile”. However, is “abnormal black bile” an existing phenomenon with distinct biological basis or merely imaginary philosophical concept that has no medical meaning? After 1H nuclear magnetic resonance spectroscopy analysis of plasma samples from cancer patients with “abnormal black bile”, non-abnormal black bile and healthy controls, here we showed cancer patients with “abnormal black bile” had similar metabolic changes and characteristics which were different from the other two groups. Thus we assumed “abnormal black bile” may have some biological basis and cancers may have some other classification and treatment methods. We selected 240 patients who had a pathologically confirmed diagnosis of 20 different cancers and staging according to AJCC system and classified them into “abnormal black bile” group and “non-abnormal black bile” group according to humor classification criteria described in Cannon of Medicine (3), while 50 healthy volunteers were used as controls. 1H nuclear magnetic resonance based metabolomics approach was used to obtain the plasma metabolic profiles of their blood samples. Fourier-transformed 1H NMR spectra of all samples were analyzed by employing partial least squares discriminant analysis (PLS-DA) by SIMCA-P 11.0 software (Umetrics, Sweden) The scores plot for healthy controls and cancer groups are completely distinct and the spatial distribution of “abnormal black bile” syndrome group differs from “non-abnormal black bile” syndrome group significantly as well. Results showed, Compared with healthy controls, “abnormal black bile” group had higher concentration levels of formic acid, unsaturated lipid, Very Low Density lipoprotein (VLDL), Low Density lipoprotein (LDL), acetone, acetate, acetoacetate, pyruvate, ß-hydrocxybutyrate, carnitine and malonic acid (P<0.001), and lower concentration levels of leucine, alanine, ß-glucose, α-glucose, inositol, creatine and methionine (P<0.05). Compared with “non-abnormal black bile” group, “abnormal black bile group” had higher concentration levels of inositol, lactic acid, choline, methionine, acetone, acetoacetate, and ß-hydrocxybutyrate (P<0.05), but lower concentration levels of alanine and α-glucose (P<0.05). These results may suggest cancer patients with abnormal black bile and with non-abnormal black bile have similar metabolic changes, but the extent of these changes is different. Concentration of acid metabolites and levels of fat mobilization and glycolysis are higher and the damage to the immune function is greater in “abnormal black bile” group, followed by “non-abnormal black bile” group and healthy controls. Therefore, based on results above, it
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may be fair to assume “abnormal black bile” may have some biological basis. Furthermore, we assumed, beside disease classification method used in modern medicine, common complex diseases such as cancers may have some other classification methods and typing possibility, and based on this classification method, there might be other treatment methods and therapeutic drugs, which need to be confirmed in further research.
A-092Bioactive fraction of Abnormal Savda Munziq and its antiproliferative activity on HL-60 cells
Murat Kizaibek1, M.Baranov 2, Halmurat Upur1*1 Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; 2Department of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia ；Correspondence should be addressed to [email protected]
Objective: 1) Study the chemical composition of ASMq and quantify its main components; 2) Find the in-vitro anticancer active components of ASMq; 3) Find preparation methods which suitable for manufacturing of different extracts from ASMq; 4) Establish a HPLC method for analyzing ASMq and its enriched active components; 5) Evaluate the in-vitro anticancer activity of single herbs of ASMq. Methods: 1) The content of total flavonoids, expressed as rutin equivalents, was measured by using the NaNO2-Al(NO3)3-NaOH colorimetric assay; The content of total phenolics, expressed as gallic acid equivalents, was measured by using the Folin-Ciocalteu assay; The content of total saccharides, expressed as glucose equivalents, was measured by the phenol-sulfuric acid method; The content of total saponins, expressed as ammonium glycyrrhizinate equivalents, was measured by the vanillin–perchloric acid method; The content of total alkaloids was measured by gravimetry. 2) Four extracts, namely Extr-1, Extr-2, Extr-3 and Extr-4, were prepared from ASMq. Among these extracts, Extr-1､Extr-2 and Extr-3 were obtained by macroporous resin chromatography as well as Extr-4 by polyamide chromatography; Thin-layer chromatography (TLC) analysis and preliminary chemical tests were performed on each extract of ASMq and the content of main components of each extract was determined; the in-vitro antiproliferative activity of these extracts on HL-60 cells were compared; the IC50 value was determined for Extr-4; Thin-layer chromatography (TLC) analyses of ASMq extracts were performed by using fast blue salt B reagent (FBS) and anisaldehyde/ sulphuric acid reagent (AS) as spray reagents, respectively. Silicagel 60 F254 plates were used as stationary phase. 3) Fractionation of Extr-4 was performed using solid phase extraction (SPE). The cell viability was determined by trypan blue exclusion method after 24h, 48h and 72h of incubation (37°C, 5% CO2). Assay was repeated in two succesive trials, each of which was performed in triplicate. 4) Accelarated solvent extraction (ASE) was applied to the extraction of single herbs in the formula of ASMq. Evaporated at 40 under reduced pressure and dried under a stream of air to obtain CH2Cl2 and℃ MeOH extract of each herb. HL-60 cells were collected during the logarithmic growth phase, plated in wells at a density of 1.0×106 cells per ml and and allowed to attach for 24 hours. The wells were divided into 19 groups. Cells in the first group were treated with blank solvent, while cells in the other 18 groups were treated with CH2Cl2 extract and MeOH extract of each herb, respectively. The final concentration of an extract of each herb in HL-60 cell medium was 100 µg/ml. The cell viability was determined by trypan blue exclusion method after 24h, 48h and 72h of incubation (37°C, 5% CO2). Assay was
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performed in triplicate. TLC analyses of herbs and ASMq extracts were performed by using fast blue salt B reagent (FBS) and natural products/ polyethylene glycol reagent (NP/PEG) as spray reagents, respectively. Silicagel 60 F254 plates were used as stationary phase. 5) The phenolic compounds exist in ASMq were analyzed by means of HPLC-DAD. A Hypersil BDS-C18 was used throughout this study. The guard column was 100 RP-18. Detection was performed at 254 nm and the absorption spectra of compounds were recorded between 190 and 450 nm. The elution solvents used were A (H2O-HAc, pH 2.8) and B (MeCN with an aliquot of HAc). Identification of compounds was achieved by comparing retention time values and UV spectra as well as spiking samples with reference compounds.Results: 1) The phytochemical screening of ASMq gave positive tests for saccharides, saponins, phenolics, flavonoids, alkaloids, coumarins and/or lactones, organic acids, amin- oacids and negative tests for tannins, anthocyanins, anthraquinones, cardiac glycosides. The content of total flavonoids, total phenolics, total saccharides and total alkaloids were measured. As a result, a good linearity between concentrations and absorbances of rutin was found in the range of 0.011～0.130 mg/ml, LOD and LOQ were estimated as 0.06 and 0.18 μg/ml respectively, recovery ranged from 95.1% to 97.5%, and precision was evaluated as 1.34%, the content of total flavonoids in the water extract and ethanol extract of ASMq were quantified as 16.2mg/g and 19.6mg/g, respectively; A good linearity between concentrations and absorbances of gallic acid was found in the range of 2.2～7.7μg /ml, LOD and LOQ were estimated as 0.01 and 0.03 μg/ml respectively, recovery was ranged from 99.5% to 104.8%, and precision was evaluated as 3.39%. The content of total phenolics in the water extract and ethanol extract of ASMq were quantified as 38.28mg/g and 21.54mg/g respectively; A good linearity between concentrations and absorbances of glucose was found in the range of 0.0023-0.0146 mg/ml, the recovery was determined to be 97.5% (n=9), and precision was evaluated as 1.34% (n=5), and the content of total saccharides in Abnormal Savda Munziq were quantified as 57.99±1.36% (n=3) ; A good linearity between concentrations and absorbances of ammonium glycyrrhizinate was found in the range of 0.0115～0.0577mg/ml, the recovery was 90.4% (n=5), and the precision was evaluated as 1.14% (n=5). The content of total saponins in Abnormal Savda Munziq were determined to be 4.69±0.08% (n=3) ; The content of total alkaloids in ASMq should be 0.44% (0.21%+0.23%) at the most. 2) The result of TLC analysis and preliminary chemical assay on ASMq extracts have indicated that Extr-1, Extr-3 and Extr-4 contain saccharides, saponins and phenolics, respectively. Extr-2 was also proved to contain phenolics and saponins, but their respective spots are not so distinct, comparing to those observed in Extr-3 and Extr-4. The quantification tests have shown that the content of flavonoids and phenolics in Extr-4 were 33.3%±0.7% and 13.9%±0.7%, respectively, whereas the content of saccharides in Extr-1 and the content of saponins in Extr-3 were 51.4%±0.9% and 60.7%±0.1%, respectively. The total amount of main components of Extr-2 has remained undetermined due to their uncertainty. Extr-4 reduced the cell viability of HL-60 cells remarkably compared with other extracts. Its IC50 value after 24 h, 48 h and 72 h cell exposure were determined to be 105.7 μg/ml, 95.1 μg/ml and 72.3 μg/ml, respectively. 3) The result of in-vitro anticancer test has revealed that all SPE fractions showed various degrees of antiproliferative activity on HL-60 cells. Among them, SPE-40 was found to exhibit the strongest inhibitory effect on the proliferation of HL-60 cells by inducing a time-dependent decline in cell viability. After spraying the TLC plates with NP/PEG reagent, all SPE fractions except SPE-80 showed yellow spots. More yellow spots were detected in SPE-40 than in other fractions. On the TLC plate of Extr-4, SPE-40 and rutin, a common spot with same color and same Rf value was detected. 4) The extraction of single herbs of ASMq was performed using ASE. Data generated from cell test clearly showed that CH2Cl2 extracts of all nine herbs and MeOH extracts of five herbs, namely
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Glycyrrhiza uralensis, Anchusa italica, Adiantum capillus-veneris, Euphorbia humifusa and Lavandula angustifolia significantly reduced the viability of HL-60 cells with a time-dependent response relationship, respectively. The antiprolifera- tive activity of CH2Cl2 extract of each herb was shown to be stronger than that of its MeOH extract. Spots belong to phenolics were detected in Anchusa italica, Adiantum capillus- veneris, Euphorbia humifusa and Glycyrrhiza uralensis. CH2Cl2 extract of Glycyrrhiza uralensis showed stronger spots compared with other extracts. Besides, Extr-4 and Glycyrrhiza uralensis have shown similar phenolic spots with same color at same position. In addition, SPE-40 and five herbs, namely Euphorbia humifusa, Adiantum capillus-veneris, Anchusa italica, Foeniculum vulgare, Melissa officinalis, have same flavonoid spots with same color (yellow) at same position. 5) Using HPLC-DAD, ①caffeic acid② rutin ③isoquercitrin ④isorhamnetin 3-O-rutinoside ⑤apigenin 7-O-glucoside ⑥rosmarinic acid⑦luteolin and ⑧formononetin were found in Extr-4. Among these phenolic compounds, 3 and 6 were identified in SPE-p; 1, 3, 5 and 6 were present both in SPE-0 and SPE-20, 2 - 6, were identified in SPE-40, whereas 4, 7 and 8, were identified only in SPE-60. Conclusion: 1) The main components of ASMq and their contents were systematically analyzed; methods of determining total phenolics, total flavonoids, total sugars and total saccharides in ASMq were established to evaluate its main composition. The result will provide a scientific basis for the fractionation and quality control of ASMq. As a result, ASMq was found to contain mainly sugars, saponins, phenolics (including flavonoids) and alkaloids. In the order of decreasing content, they can be arranged as sugars, saponins, phenolics and alkaloids. The sum of whole amounts of total saccharides, total saponins and total phenolics account for two thirds of whole weight of ASMq. 2) Four extracts, namely Extr-1, Extr-2, Extr-3 and Extr-4, were prepared from ASMq. The qualification and quantification assays have revealed that the main components of Extr-1, Extr-3 and Extr-4 were polysaccharides, saponins and phenolics, respectively. The preparation method of each extract was found to be simple and suitable for industrialization. In-vitro anticancer test and TLC analysis have implied a possible correlation between phenolic compounds and their antiproliferative activity on HL-60 cells. Among the four extracts, Extr-4 was shown to possess the strongest antiprolifertive activity on HL-60 cells. 3) Extr-4 was further fractionated into six subfractions by using solid phase extraction. Among these fractions, SPE-40 has shown the strongest inhibitory effect on HL-60 cells. The result of TLC analysis revealed the presence of more flavonoids in this fraction than in any other fractions, indicating that flavonoid could be an active component or one of the active components in ASMq. 4) HPLC-DAD analysis has revealed the presence of caffeic acid, rutin, isoquercitrin, isorhamnetin 3-O-rutinoside, apigenin 7-O-glucoside, rosmarinic acid, luteolin and formononetin in Extr-4. Among these phenolic compounds, rutin, isoquercitrin, isorhamnetin 3-O-rutinoside, apigenin 7-O-glucoside and rosmarinic acid were also exist in SPE-40 (the most active SPE fraction). In conclusion, the bioassay guided fractionation of ASMq led to the isolation of the most active fraction rich in phenolics.
A-093Effect of Xiatara on ultrastructure, protein and ergosterol biosynthesis in dermatophytes
Deng Shuwen1, Halmurat Upur2*1Second Army University; 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;
*Correspondence should be addressed to [email protected]
Objective: 1) To evaluate antifungal susceptibility of Xiatare（XTR） against dermatophytes, we
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determined antifungal susceptibility of Xiatare against dermatophytes with M38-P and yeasts with M27-P. We also first established NCCLs M27-P and M38-P method in Xinjiang Medical University. 2) To investigate the effect of Xiatare on ultrastructure of dermatophytes This paper observed ultrastructure of T.r and T.m treated by Xiatare and compare to control group. The object is to find the antifungal target of Xiatare. 3) To investigate the effect of XTR on fungal protein. To find target of Xiatare to fungus with SDS-PAGE. 4) To investigate effect of Xiatare, a new antifungal agent on ergosterol biosynthesis in fungi. We know from SEM, TEM and SDS-PAGE that Xiatare had the effect on fungal cell membrane and wall and protein. We just want to study how Xiatare inhibit ergosterol biosynthesis in fungi by the method of GC/MS. Method: 1) Fungus Strains all came from center of fungus and mycoses research in Xinjiang Medical University (XYZ). Xiatare was given by haboqikang Pharmacy Co. as testing drug, tebinafine was given by Novatis Pharmacy Co. as positive drug. We examinated antifungal susceptibility of Xiatare against dermatophytes with M38-P and to yeasts with M27-P. 2) T.r 400017 and T.m 60002 are observed, two strains come from XYZ, testing drμg is Xiatare, given by Qikang Pharmacy Co.. Positive drug is terbinafine given by Novitis Pharmacy Co.. Fungus treated according NCCLs M38-P. There are three groups. One is control(just fungus, no drug treated ), one group is fungus treated by XTR(concentration: 256 μg/ml、128 μg/ml、64 μg/ml、32 μg/ml) and one group is fungus treated by TBF(concentration: 0.01 μg/ml、0.005 μg/ml、0.0025 μg/ml、0.00125 μg/ml). 3) T.r 400017 are observed, strain come from XYZ, testing drug is Xiatare given by Qikang Pharmacy Co. There are two groups of which one is fungus with no treatment, and the other is fungus treated by Xiatare. dermatophytes strains were grown at 30°C in RPI1640 medium and grown for approximately 17-14days. Protein extracted in fungi is prepared according general method. SDS-PAGE is routine method that have been previously described. 4) T.r 400017are observed, strain come from XYZ, testing drug is Xiatare given by Qikang Phar.Saponification and extraction of the nonsaponifiable (NSLs) of fungi were carried out after 24h of incubation with 256μg/ml concentrations of Xiatare .Erogesterol in NSLs were separated and their concentration were determined by the method of GC/MS .Sterol extraction and analyses according to the method of Bligh and Dyer. Nonsaponifiable sterol analyses using GC-MS. Result: 1)① Antifungal susceptibility of Xiatare against 171 strains dermatophytes had been showed. The MIC90 of Xiatare to T.r is 1024μg/ml, Mean MIC is 907μg/ml; The MIC90 of Xiatare against T.m is 1024μg/ml, Mean MIC is 1000μg/ml; The MIC90 of Xiatare against T.vio is 1024μg/ml, Mean MIC is 1047μg/ml; The MIC90 of Xiatare against T.ton is 1024μg/ml, Mean MIC is 1023μg/ml; The MIC90 of Xiatare against M.g is 2048μg/ml, Mean MIC is 1862μg/ml; The MIC90 of Xiatare against M.canis is 2048μg/ml, Mean MIC is 2048μg/ml; The MIC90 of Xiatare against E.f is 1024μg/ml, Mean MIC is 1023μg/ml. ②Antifungal susceptibility of Terbinafine against 171 strains dermato- phytes had been showed. The MIC90 of Terbinafine against T.r is 0.01μg/ml, Mean MIC is 0.0093μg/ml; the MIC90 of Terbinafine against T.m is 0.01μg/ml, Mean MIC is 0.01μg/ml; the MIC90 of Terbinafine against T.vio is 0.01μg/ml, Mean MIC is 0.01μg/ml; the MIC90 of Terbinafine against T.ton is 0.01μg/ml, Mean MIC is 0.01μg/ml; the MIC90 of Terbinafine against M.g is 0.08μg/ml, Mean MIC is 0.08μg/ml; the MIC90 of Terbinafine against M.canis is 0.005μg/ml, Mean MIC is 0.005μg/ml; the MIC90 of Terbinafine against E.f is 0.04μg/ml, Mean MIC is 0.0398μg/ml. ③Antifungal susceptibility of Xiatare against 69 strains cadidas had been showed in which the MIC90 of Xiatare against C.a is 16384μg/ml, Mean MIC is 14588μg/ml; the MIC90 of Xiatare against C.tro is 4096μg/ml, Mean MIC is 3614μg/ml; the MIC90 of Xiatare against C.gla is 16384μg/ml, Mean MIC is 15135μg/ml; the MIC90 of Xiatare against C.kr is 16384μg/ml, Mean MIC is 13489μg/ml; the MIC90 of Xiatare against C.par is 16384μg/ml, Mean MIC is 16384μg/ml. Antifungal susceptibility of Terbinafine against 69 strains candidas had been showed. The
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MIC90 of terbinafine against all candidas is 0.5μg/ml, Mean MIC is 0.5μg/ml. ④Minimum fungicidal concentration (MFC)of Xiatare against 7 strains dermatophytes had been showed. 7 strains dermatophytes all growed. Minimum fungicidal concentration (MFC)of Terbinafine against 7 strains dermatophytes had been showed. 7 strains dermatophytes all didn’t grow. 2) ① EM result of Xiatare against T.r. Control group, SEM: hyphae and spore grow very nice. TEM: cross section of hyphae is whole, we can see cell core in cell. Treated by Xiatare, SEM: as concentration increasing, hyphae swell, rupture. TEM: as concentration increasing, cell membrane crimple, some cell wall was damaged, substance in cell go out of cell. Treated by Terbinafine, SEM: as concentration increasing, hyphae swell, dry. TEM: as concentration increasing, cell membrane crimple, shriveled. substance in cell go out of cell. ②EM result of Xiatare to T.m. Control, SEM: hyphae and spore grow very nice. TEM: cross section of hyphae is whole, we can see cell core in cell. Treated by Xiatare, SEM: as concentration increasing, hyphae swell,rupture. TEM: as concentration increasing, cell membrane crimple, some cell wall was damaged, substance in cell go out of cell. Treated by Terbinafine, SEM: as concentration increasing, hyphae swell,dry. TEM: as concentration increasing, cell membrane crimple, shriveled. substance in cell go out of cell. 3) From result of SDS-PAGE, to compare to control, protein strip is weaken in 45 kDa, 35 kDa, 25 kDa, t with a dose –dependent manner in fungi. 4) ①Qualitative analysis of erogsterol. There is peak in 40.94min in ion chart, the ion intension is 4.73e4 and also we search it in Wiey and Nist bank to find it is the ion intension. There is peak in 40.50min in ion chart, the ion intension is 1.82e3 and also we search it in Wiey and Nist bank to find it is erogsterol. ②Quantitative analysis of erogsterol. Quantity of Erogsterol of dermatophytes treated by XTR less 35.11% than control one. Conclusion: 1) XTR have strong antifungal susceptibility to dermatophytes, MIC90 is 1024μg/ml. Antifungal table is T.rubrum T.mentagrophytys. T.tonsurance. E.floccosum、T.violaceum，M.gypseum、M.canis in turn. 1024～2048μg/ml is sensitive range. XTR is a drug inhibited fungus. 2) We can know from SEM and TEM that XTR can affect on fungal cell wall and cell mambrance. 3) In the base of SDS-PAGE, XTR can inhibite protein biosynthesis of Dermatophytes, and it is more than one protein affected by XTR. 4) We can know from GC/MS results that after treated by XTR, Quantity of Erogsterol of dermatophytes treated by XTR less 35.11% than control one.
A-094Establish the Alzheimer's disease carrying abnormal savda syndrome rat model and observe the effects of formulae on it
Palidan Wushuer1, Nurmuhammat Amat2*, M.Baranov, Halmurat Upur3*1Affiliated Traditional Medicine Hospital, Xinjiang Medical University, 830000, Urumqi, Xinjiang Uighur Autonomous Region
China; 2Department of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia 3Traditional Uighur
Medicine institute, Xinjiang Medical University, Urumqi, Xinjiang Uighur Autonomous Region，China; *Correspondence to:
[email protected] or [email protected]
Objective: To Establish the Alzheimer's disease carrying abnormal savda syndrome rat model and observe the effects of abnormal savda munziq on it. Methods: Establish abnormal savda syndrome rat model using classical method, then injected β-Amyloid 1-40 on hippocampus to establish the Alzheimer's disease carrying abnormal savda syndrome rat model, and the model group was given abnormal savda munziq, after observing the biological characterization and testing the behavioral changes with Morris water maze test and Step down test, compared with the abilities of learning and
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memorizing of the different model groups. Result: Compared with the normal group, the daily food and water intake were increased in abnormal savda syndrome group and the Alzheimer's disease carrying abnormal savda syndrome group(P<0.01), the body weigh was decreased in abnormal savda syndrome group and the Alzheimer's disease carrying abnormal savda syndrome group(P<0.01); the abilities of learning and memorizing were weaken in the AD group、abnormal savda syndrome group and the Alzheimer's disease carrying abnormal savda syndrome group(P<0.05); the biological characterization, the abilities of learning and memorizing were improved in the Alzheimer's disease carrying abnormal savda syndrome group + abnormal savda munziq group (P<0.05). Conclusion: In this research the Alzheimer's disease carrying abnormal savda syndrome rat model was established, abnormal savda munziq could improve biological characterization、 the abilities of learning and memorizing of the Alzheimer's disease carrying abnormal savda syndrome rats.
A-095Establishment of hepatocarcinoma carrying abnormal savda animal model and it`s morphological
Yusup Tuerxun1, Halmurat Upur1*1Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region，China; *Correspondence should be addressed to [email protected]
Objective: To establish combining hepatocarcinoma disease and abnormal savda syndromes rats model, and investigate the effect of abnormal savda on inducing and developing process of hepatocirrhosis phase in this model. Methods: Under the direction of Traditional Uygur Medicine, multi complcated induction methods were used to establish abnormal savda syndromes rats model for three weeks, using DEN further to establish combining hepatocarcinoma and abnormal savda disease rats model till hepatocirrhosis occurs, observe dynamically the surface of the liver and it’s pathological and ultrastructural changes in 5th, 7th, 9th, 11th weeks respectively. Results: Both in the model control group and combining disease and abnormal savda syndromes model group there are the pathological changing process of hepatoxic lesion, hepatic proliferation, hepatocirrhosis, but the pathological changes of the liver surface in the combining disease and abnormal savda syndromes model group is faster than model control group. At the same time point, the dropsical level of liver cell, typeless hyperplasia, fake foliole level in liver, the ultrastructural changes of decreasing hepatin and chondriosome in liver cells in combining disease and abnormal savda syndromes model group are heavier than those in the model control group. Conclusion: These findings indicate that in the process in which establishing abnormal savda disease rat model serving as a base, using DEN further to establish combining hepatocarcinoma disease and abnormal savda syndromes rats model, the abnormal savda hilit accelerates the inducing and developing process of hepatocyte to hepatic Cellular proliferation to hepatocellular carcinoma and this process is similar to the process of humans hepatocarcinoma. It is also stable and reliable.
A-096
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Experimental Study on Caries Prevention by Aleppo Gall
Z.Jin 1, Halmurat Upur 2* , M.Baranov 3
1First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China; 2Traditional Uighur Medicine Institute, Xinjiang
Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; 3Department of Hospital Therapy, Pavlov's
Medical University, Saint-Petersburg, 197000, Russia; *Correspondence should be addressed to [email protected]
Objective: The aim of this study is to use latest method and technique of Traditional Chinese Medicine to study preventions of cariogenic bacteria and oral bacterial biofilm formation by using Aleppo Gall and active compound, at the same time, the aim of this study was to evaluate the anticaries efficacy of Aleppo Gall and active compound on dental caries formation and plaque microbiology in rats. Methods: 1) A series of in vitro anticarious experiments, inhibition of growth, acid production, glucan generation, have been carried out. 2) The visible light semiquantitative method has been used to measure biomass (OD570) values so that the effect of medicine on biomass of oral bacteria biofilm has been discovered. Idio-fluorochrome labeled mortuary bacteria and aliving bacteria; fluorescence microscope has been used to observe morphosis and activity of treated-oral bacterial biofilm and untreated-oral bacterial biofilm. 3) The caries rats models were established, so that to evaluate the anticaries efficacy of medicine on dental caries formation and plaque microbiology in rats. Results: 1) Anticarious experiments, including the inhibition effect of growth, acid production, glucan generation and initial adherence are effective. 2) It was discovered that Aleppo gall and active components of Aleppo gall were able to inhibit bacteria of dental biofilm, compared with negative control, it had significant (P<0.05). The effect of Aleppo gall and active components of Aleppo gall on oral bacterial biofilm not only inhibits bacteria biofilm growth, but also adjusts biofilm structure. 3) Streptococcus mutans level: Compared with the distilled water, and the rest of the group to different extents in both strains decreased, only NaF and CHX group compared with the distilled water there is significant difference (P <0.05); caries Keyes points: the experimental group of drugs on the formation of dental caries in rats and the severity of the suppression in varying degrees, of whichTurkish gall show on sulcal-surface and smooth-surface caries has a good effect (P <0.05), but were lower than the NaF group; Turkish Gall + NaF group had no obvious role in the synergistic inhibition of caries. Conclusions: The inhibition effect of growth, acid production, glucan generation and initial adherence are effective; The effect of Aleppo gall and active components of Aleppo gall on oral bacterial biofilm not only inhibits bacteria biofilm growth, but also adjusts biofilm structure; The natural medicine inhibits rat oral Streptococcus mutans growth and metabolism, effectively prevent the occurrence of dental caries.
A-097General toxicology studies of Xi pa yi Kui Jie’an enema
Kasimjan Aximjan 1, Halmurat Upur1*1Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region，China; *Correspondence should be addressed to [email protected]
Objective：Kui Jie’an enema (KJA), Uighur medicine has been used in the treatment of ulcerative colitis (UC). However, there is very little information regarding the toxicity of KJA to give an assurance of safety for clinical treatment. To provide safety information for KJA, we evaluated its acute toxicity in
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mice, sub-chronic toxicity in rats and rectal irritation in rabbits. Methods: The single-dose toxicity of KJA was examined using ICR mice. Mice were treated with KJA extract rectally at 0 and 10 g/kg body weight. After a single administration, clinical signs, body weights, food and water consumption were observed every day for two weeks. In the sub-chronic rectal toxicity study, KJA was administered to rats at 0, 2, 0.8, or 0.2 g/kg/day for 3 and 6 months after discontinuation lasted for a period of 4 weeks convalescence time. Mortalities, clinical signs, body weight changes, food and water consumption, ophthalmologic findings, urinalysis, hematological and biochemical parameters, gross findings, organ weights, and histological examination were monitored during the study period. In the rectal irritation test, KJA was administered to rabbits at 0.38、0.04g/kg/day for 14 days. Mortalities, body weight changes, food and water consumption, excretion were monitored during the study period. Results: We found no mortality and no abnormalities in clinical signs, body weights, and necropsy findings for any of the animals in the acute studies following rectal administration in the rat at up to 10 g/kg/day KJA. In the sub-chronic rectal toxicity study shows that, no significant changes in general behavior and hematological and biochemical parameters, except for Cl、MCV、MCHC、TG、CK、GLB、HGB、T-BI、TP、ALT and AST parameters. No significant changes were observed in organ weights, and histopathological results showed normal profile suggesting no morphological alterations. In the Rectum mucosal stimulating degree in rabbits belong to without excitant range, and not found any morphological alterations in colon and rectum. Conclusion: The present findings indicated that KJA do not pose any significant acute toxicity in mice and chronic toxicity in rats and may not rectal irritation.
A-098Abnormal Savda Munziq and Munshil’s effect on the gene regulation, oxidative stress induced cell damage and apoptosis
Li Lin 1, Lgor Gogol 2, Halmurat Upur 3*
1Biochemistryt Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region ， China; 2Department of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia 3Traditional Uighur Medicine
Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China, *Correspondence should be addressed to [email protected]
Objective: 1) To investigate the effects of Abnormal Savda Munziq and Munshil on the H2O2-induced DNA damage and p53 protein expression in lymphocyte. 2) In order to investigate the influence of Abnormal Savda Munziq and Munshil on H2O2–induced lymphocyte apoptosis and bcl-2, NF kappa B protein expression. 3) To investigate the influence of Abnormal Savda Munziq and Munshil on the transcription of p53, bcl-2 and Fas of Hela cells. Methods: 1) In this study, single cell gel electrophoresis (SCGE) technique and MTT assay were used to measure the DNA damage and cytotoxicity of lymphocyte induced by hydro-peroxide. 2) The cultivated lymphocytes were incubated with different concentrations of H2O2, the apoptosis index (AI) was detected by Hoechest 33258 fluorescence dying method and the electron microscopy to determine the influence of different does of Abnormal Savda Munziq and Munshil on oxidative stress induced apoptosis. In the same experiment, Western blot was employed to determine the expression of bcl-2 and NF-kappa B p65 proteins in different treatment lymphocyte groups. 3) The mRNA of p53, bcl-2 and Fas of the Hela cells treated with Abnormal Savda Munziq and Munshil were detected using the RT-PCR method. Results: 1) The results
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of SCGE and MTT assay showed that the mark of DNA damage and cytotoxicity increase in a concentration dependent manner as the H2O2 concentration elevated in the different groups. The Munziq of Abnormal Savda display significant protective effect against DNA damage and inhibition of cytotoxicity induced by 800μmol/L H2O2 the value of DNA damage mark decrease in does dependent manner as does of Abnormal Savda Munziq elevate. 2) The results show that lymphocyte group treated with 100μmol/L H2O2 for 6hr result in the highest apoptosis index in this study. Both of Abnormal Savda Munziq and Munshil can decrease apoptosis index induced by hydro –peroxide at high does, especially Munziq at 1000μg/ml can greatly decrease it. Abnormal Savda Munziq can inhibit. H2O2-induced lymphocyte apoptosis while high does Munshil also. Western blot the result show, H2O2 treatment (1000mol/L,6h) result in a high expression of NF-kappa B p65 protein, but this can be inhibited by both of Abnormal Savda Munziq and Munshil at dose of 1000μg/ml. 3) The RT-PCR method result revealed that there were no bcl-2 and Fas transcriptants apperence in the Munziq and Munshil treated Hela cell groups, just same as that of control group. But p53 transcriptant is significantly increased in Munziq and Munshil treated Hela cell groups comnpared with that of cortrol group, which appears no p53 transcriptant. Conclusion: 1) The low does of Abnormal Savda Munshil show weak protective effect against hydro-peroxide induced DNA damage while high does makes the damage even worse. 800μmol/L H2O2 treatment of lymphocyte makes the p53 protein greatly elevated and this effect can be slightly inhibited by the pre-incubation with both Abnormal Savda Munziq and Munshil. 2) The Abnormal Savda Munziq itself also show a obvious up-regulation upon lymphocyte bcl-2 protein expression. So it indicate the Abnormal Savda Munziq can up-regulate the lymphocyte bcl-2 protein expression. Contrast to Abnormal Savda Munziq, the Abnormal Savda Munshil shows the opposite effect that can inhibit the lymphocyte bcl-2 protein expression. It suggest that the Abnormal Savda Munziq’s up-regulation of bcl-2 protein may be associated with it’s inhibition effect of lymphocyte apoptosis induced by hydro-peroxide. 3) It indiciated that this may be related to the Munziq and Munshil’s capability to induce apoptosis of cancer cell.
A-099Mechanisms research of Gujingmaisiha treatment for premature ejaculation (PE)
Song Guohong1, S.Nelski 2, Halmurat Upur3*1Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,China; 2Heidelberger Traditional Chinese medicine Physiccal therapy Institute, Zwingenberg,Germany;3Traditional Uighur Medicine
Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should be
addressed to [email protected]
Objective: Observeing the function of the Xinjiang Uighur Medicine Gu-jing-mai-si-ha to the premature ejaculation after treating medically, studying the parts of function mechanism of the Xinjiang Uygur medicine gu-jing-mai-si-ha treatment premature ejaculation. Method: Pass the random method observation Gu-jing-mai-si-ha treatment in front and back premature ejaculation sufferer the premature ejaculation related questionnaire and intravaginal ejaculation latency time (IELT) and observe the lever of nitric oxide and the prostaglandin F2a in blood plasma, and then compare with the controls. Result: Cure 15 diva the level of nitric oxide, prostaglandin F2a in blood plasma were significantly higher in treatment group than that of the control group, P＜ 0.001; the intravaginal ejaculation latency time (IELT), Chinese index of sexual function for ejaculation (CIPE) the questionnaire grade point after
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treatment also increases than before P＜0.001. Before treatment the level of nitric oxide, prostaglandin F2a in blood plasma, intravaginal ejaculation latency time (IELT), Chinese index of sexual function for ejaculation (CIPE), have no significantly change between the two group, P＞0.05. Conclusion: The mechanism of Gu-jing-mai-si-ha curing premature ejaculation is possibly to strengthen the interaction of the relative smooth muscle homeostasis in the urethra prostate gland department by increasing the expression of the nitric oxide, prostaglandin F2a level in blood plasma, to improve a sufferer to be subjected to the prostate gland urethra pressure chamber effect, to postpone the ejaculation arrival of urgent feeling.
A-100Metabonomic studies of abnormal Savda syndrome complexity diseases in traditional Uygur Medicine
Xu Wei fang1, N.Tatiana 2, T.Aji 3,Upur Halmurat4*1 Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region, China; 2Department of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia; 3Cardiovacular Diseases Department,Bortala People Hospital ； 4Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region , China; *Correspondence should be addressed to
Objective: In this study, we investigated plasma of complexity diseases (neoplasm, diabetes and bronchial asthma) patients with abnormal Savda syndrome using nuclear magnetic resonance spectroscopy (NMR), and described the metabolic variety and characteristics of complexity diseases with abnormal Savda syndrome. Methods: 1) 50 healthy volunteers and 223 cases of abnormal Savda syndrome neoplasm patients which are confirmed clinically were enrolled in this study. 2) 50 healthy volunteers and 130 cases of Type 2 diabetes enrolled in this study. 3) 40 healthy volunteers and 39 cases of abnormal Savda syndrome patients with asthma enrolled in this study. Blood collection from each individual was carried out in the morning before breakfast. 1H NMR spectra from each samples were recorded on nuclear magnetic resonance spectroscopy. Fourier transformed 1H NMR spectra were corrected for phase and baseline distortions. The integral results of 1H NMR spectra were performed with orthogonal projection to latent structure with discriminant analysis (OPLS-DA). Results: 1) Neoplasm: In plasma of abnormal Savda syndrome neoplasm patients a wide range of amino acids including Leucine, Alanin, Citruline, Tyrosine, Histidine, Glycine, Methionine, Isoleucine, Valine, Acetylcysteine decreased significantly (p<0.05)and Glycoprotein, Glutamine, Myo-inositol, Lactic acid, Choline Creatine also decreased (p<0.05). Formic acid, Acetone, Acetic acid, Acetoacetate, Pyruvate, β-Hydrocxy butyrate, Carnitine, Malonic acid increased compared with healthy plasma metabolites(p<0.05). 2) Diabetes: The patients of Type 2 diabetes with abnormal Savda syndrome had high concentration of glucose, very low density lipoprotein, unsaturated lipid and acetone in blood compared with healthy controls (p<0.05), but low concentration of isoleucine, leucine, valine, alanine, glutamate, histidine, 1-methyl histidine, tyrosine, phenylalanine, glycine, citric acid, lactate, scyllo-inositol, creatine, creatinine and glycoprotein (p<0.05). 3) Asthma: In plasma of abnormal Savda syndrome patients with asthma the expression of isoleucine , leucine , valine , lactate , alanine , glycoprotein , citrate , creatine , creatinine , 1-methy histidine , scyllo-inositol see a significant decrease comparing with healthy group (p<0.05) , whereas , the expression of acetone , acetoacetate , carnitine
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significant elevated (p<0.05). Conclusion: The patients who had Abnormal Savda syndrome complexity diseases by the theory of Traditional Uygur Medicine like neoplasm, diabetes and bronchial asthma had same metabolic change as they are “different disease same syndrome” . In this study, abnormal Savda patients had similar metabolic change compared to healthy controls. Abnormal Savda syndrome patients with lipid metabolism-related metabolites increased as well as abnormal amino acid metabolism, cytoplasm membrane defected, oxidative damage will occurred by abnormal antioxidation ability. These findings indicated that when the body form the abnormal Savda syndrome, it is possible to accrued the energy metabolism abnormality, low immune function, which led to various diseases such as neoplasm, diabetes and bronchial asthma. These findings would facilitate further understanding of abnormal savda syndrome in Traditional Uygur Medicine is a special state of complexity diseases.
A-101Studies on platelet parameters and hepatic function changes of COPD patients with syndrome of kidney deficiency and stagnation of phlegm
You Na 1, Halmurat Upur2*1Xi’an An Traditional Chinese Medicine Hospital, 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region , China; *Correspondence should be addressed to [email protected]
Objective: To investigate the distribution of COPD with syndrome of kidney deficiency and stagnation of phlegm (KDSP) and the clinical significance of platelet parameters and hepatic function changes. Methods: 103 patients of AECOPD belonging to Severity Ⅲ～Ⅳ were divided into two groups, KDSP group and non- KDSP group, according to the differentiation of symptoms and signs for classification of syndrome. Five platelet parameters of COPD patients, including PLT, PDW, P-LCR, PCT and MPV were detected by automatic cytoanalyze; six serum biochemical indicators of COPD patients, including TBIL, GPT, ALP, TP, ALB and GLB were detected by automatic biochemistry analyzer. Results: 1. Among 103 AECOPD patients observed, there were 70 cases in KDSP group, taking a percentage of 68%; and 33 cases in non-KSPD group (including 8 cases with obstruction of the lung by phlegm, 10 cases with pulmonary retention of phlegmopyrexia, 3 cases with mental confusion caused by phlegm, 7 cases with syndrome of water over-flowing due to YANG-insufficiency, 5 cases with insufficiency of QI of the lung and kidney), taking 32%. There was no statistical significance between KDSP group and non-KDSP group about gender (P>0.05); and compared with non-KDSP group, the patients in KDSP group were elder, the difference of which had statistical significance (P<0.05). 2. Compared with the control group, the PLT levels of KDSP group and non-KDSP group both significantly decreased (P<0.01, P<0.05), and the decreased level of KDSP group was more significant. The PDW levels of KDSP group and non-KDSP group both significantly increased (P<0.01, P<0.01), and the increased level of KDSP group was more significant. The PCT levels of KDSP group and non-KDSP group both significantly decreased (P<0.01, P<0.05). The P-LCR level of KDSP group significantly increased (P<0.05), while the P-LCR level of non-KDSP group also increased, but had no statistical significance (P>0.05). There was no significant difference among the groups, the control group, KDSP group and non-KDSP group about the levels of MPV (P>0.05). 3. Compared with the control group, the TBIL level of KDSP group significantly increased (P<0.01), while the TBIL level of non-KDSP group also increased, but had no statistical significance (P>0.05); the ALT levels of KDSP group and non-KDSP group both significantly increased (P<0.01, P<0.05), while the ALT level of KDSP group increased more
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significantly; the ALB levels of KDSP group and non-KDSP group both significantly decreased (P<0.01, P<0.05), while the ALB level of KDSP group decreased more significantly. There was no statistical significance among the groups, the control group, KDSP group and non-KDSP group about the levels of ALP, TP and GLB. Conclusion: 1. KDSP is possibly one of the main patterns of syndrome of AECOPD belonging to Severity Ⅲ～Ⅳ in Xinjiang, and the elder people are susceptible to COPD with syndrome of KDSP.2. AECOPD patients belonging to Severity Ⅲ～Ⅳ appear platelet parameters abnormality, and AECOPD patients with syndrome of KDSP appear more significantly. Thus, AECOPD patients belonging to Severity Ⅲ～Ⅳ , especially AECOPD patients with syndrome of KDSP are probably susceptible to appearing platelet aggregation; and they probably have the tendency of supervening thromboembolic disease. 3. Dynamic observation of platelet parameters changes of COPD patients possibly conduces to early diagnosis of COPD with thromboembolic disease. Early intervention prevents the aggravation of COPD. 4. AECOPD patients belonging to Severity Ⅲ～Ⅳ present hepatic function abnormality, and AECOPD patients with syndrome of KDSP appear more obviously. It is suggested that active symptomatic treatment and close attention to hepatic function changes of AECOPD patients belonging to Severity Ⅲ～Ⅳ, especially AECOPD patients with syndrome of KDSP should be emphasized, in order to guiding the clinical rational administration and treatment to AECOPD. More researches are approaching.
A-102Studies on the Anticancer and Reversing Multidrug resistance effects of Abnormal Savda Munziq Total Flavonoids
Maynur Eli 1, M.Baranov 2, Halmurat Upur3*1Tumor Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region,China; 2Department of Hospital Therapy, Pavlov's Medical University, Saint-Petersburg, 197000, Russia; 3Traditional
Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,China, *Correspondence should be addressed to [email protected]
Objective: 1) To investigate the effect of Abnormal Savda Munziq Total Flavonoids on the proliferation, apop-tosis in Caco-2. 2) In order to investigate the effects of Abnormal Savda Munziq Total Flavonoids on DMH induced colon carcinogenesis. 3) To investigate the effect of Reversal mechanism of resistance to 5-Fu in human hepatocar-cinoma cell line BEL/Fu by abnormal Savda Munziq Total Flavonoids. Methods: 1) The effect of ASMq-TF, on the proliferation of Caco-2 cells after exposure to different concentration and different time was evaluated by MTT. Apoptosis rate was determined by using flow cytometry, DNA fragmentation assay. 2) Obserb number of aberrant crypt foci (ACF) and aberrant crypt (AC) and crypt multiplicity. 3) Sentivity to 5-Fu of BEL-/Fu cells was assessed by MTT assay. The reversal action of ASMq-TF, IC50 value and synergic action of vincristine and ASMq-TF were determined by MTT assay. The reversal action of ASMq-TF was also observed by clone forming text. MDR1 expression of Pgp was examined by immunochemical assay and determination of gene expression of MDR1, MRP, glutathione S-transferase(GSTpi) and topoisomerase α of Bel-7402 cellⅡ line were investigated using reverse transcription polymerase chain reaction (RT-PCR). Expression of bcl-2 protein and percentage of cell apoptosis was detected by flow cytometry. MDR1 expression of Pgp was examined by immunochemical assay. Results: 1) The results of MTT assay demonstrated that water soluble of ASMq-TF ethanol extract showed different effects on Caco-2 cell grows at 48h and72h
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incubation. The IC50, they were IC50 5.99mg/ml(48h)and 3.02mg/ml(72h). DNA fragmentation observed by gel electrophoresis showed that there were no significant DNA ladders in Caco-2 after treatment with different water soluble of ASMq-TF 0.5~5.0mg/ml , and seems to have no detectable difference between control cultures. 2) The results showed that all doses (400, 800 and 1600 mg/kg b. w.) of ASMq-TF ethanol extract in the pre-treatment group as well as in the treatment group significantly reduced the number of aberrant crypt foci (ACF), aberrant crypt (AC) and crypt multiplicity (P<0.05), the organ relative weights in pre-treatment and treatment groups were regained by ASMq-TF ethanol extract administration when compared to negative control group (P<0.05). 3) The reversal effect of ASMq-TF was evaluated using Bel/Fu cell line with innate resistance in this study. The IC50 value of ASMq-TF crude extract was 2000.68μg/ml. ASMq-TF at 390μg/ml concentration was no toxic but had a toxic effect on Bel/Fucell line with 5-FU. The cytotoxicity of 390μg/ml ASMq-TF and 5-FU to Bel/Fu cellline was more remarkable than only with 5-FU (P<0.01). The fold reversal of ASMq-TF combining with 5-FU was 7.8.While ASMq-TF concentrations lower than 390μg/ml had less toxic, it had no synergetic effect with 5-FU. ASMq-TF concentrations higher than 400μg/ml were not suitable due to cytotoxicitiy to tumor cell. Conclusion: 1) To study the acting mechanism of the fractions of ASMq-TF ethanol extract, flow cytometry was used to detect the cell cycle distribution of Caco-2 cells after 48h treatment with each fraction, respectively. The percentage of HepG2 cells in phase sub-G1 was most high concentration of 7.5mg/ml. 2) These findings suggested that ASMq-TF ethanol extract had a chemoprotective effects on DMH-induced colon carcinogenesis, by suppressing the development of preneoplastic lesions, and probably exert protection against the initiation and promotion steps of colon carcinogenesis. 3) We concluded that the anticancer properties of ASMq-TF was possibly mediated through multiple pathways, suggesting multiple ingredients, rather than single component The acting ingredients in ASMq-TF that exerted the anticancer effect may include total flavonoids which are abundant in ASMq-TF.
A-103Study on anti-tumor mechanism of Abnormal Savda Munziq in vivo based on transplanted S-180 and EAC tumor model
Anwar Aikemu1, Halmurat Upur2*1Pharmaceutical Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,China;2Traditional
Uighur Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,China;
*Correspondence should be addressed to [email protected]
Objective: This study aims to explore the inhibitive effect of abnormal savda munziq on two kinds of transplanted tumor, protective effect of abnormal savda munziq on chemotherapy by reducing toxicity, synergistic attenuation of abnormal savda munziq on fluorouracil and further identify anticancer effect, the anti-fatigue and hypoxia resistance effect of abnormal savda munziq in vivo by hypoxia and pole climbing experiment. Methods: 1) Establishment of transplanted S-180 tumor model：180 mice were randomized to 6 groups: normal control group, tumor control group, high-dose group of ASMq, middle dose group of ASMq, low dose group of ASMq, cyclophosphamide (CY) control group. Indexes are measured in batch. Mouse tumor inhibition rate, spleen index, thymus index and the index of liver are measured. Variation of mouse serum TNF-α, IL-1β, IL-2, IFN-γ, NK, LPO are observed. Pathological conditions of Tumor in mice spleen and other organs are examined by HE staining. By the MTT method,
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the proliferation of spleen lymphocytes in mice and variation of serum lymphocyte subsets such as CD3+, CD4+, CD8+, CD4+/CD8+ are identified. 2) Establishment of transplanted S-180 tumor model: 60 mice were randomly divided into 6 groups: normal control group, tumor control group, 5-fluorouracil (5-FU) group, high dose of 5-FU ASMq group, middle dose of 5-FU ASMq group, and low dose of 5-FU ASMq group. Variations of mouse tumor inhibition rate, indexes of thymus, spleen and liver, mouse serum SOD, MDA, GSH-PX are observed. 3) Establishment of transplanted EAC tumor model: 180 mice were randomly divided into six groups, normal control group, tumor control group, high-dose group of ASMq, middle dose group of ASMq, low dose group of ASMq , cyclophosphamide (CY) control group. Observation the mouse tumor inhibition rate, spleen index thymus index, and the index of liver, mouse TNF-α, IL-1β, IL-2 , IFN-γ, NK, LPO content in serum and HE staining examination of tumor in mice spleen and other organs pathological conditions. Determination of the proliferation of murine spleen lymphocytes by MTT method and mouse serum lymphocyte subsets in CD3+, CD4+, CD8+, CD4+/CD8+. 4) Establishment of the transplanted EAC tumor model: 60 mice were randomly divided into 6 groups: normal control group, tumor control group, 5-fluorouracil (5-FU) group, high dose of 5-FU ASMq group, middle dose of 5-FU ASMq group, and low dose of 5-FU ASMq group. Variations of mouse tumor inhibition rate, index of spleen, thymus, and liver, mouse serum SOD, MDA, GSH-PX are observed. 5) Establishment of chemotherapy drug toxicity model: 50 mice were randomly divided into 5 groups: normal control group, tumor control group， AF（ amycin and 5-fluorouracil (5-Fu) group, AF+ASMq high dose group, AF+ASMq middle dose group, and AF+ASMq low dose group. Spleen, liver and thymus index，mouse serum ALT, AST, TP content, SOD in mouse heart, MDA, GSH-PX content are observed and pathologic conditions of mouse heart, liver and other viscera are examined by HE staining methods. 6) Establishment of transplanted S-180 tumor model and EAC tumor model respectively: ASMq effect on mice serum SOD, MDA, GSH-PX indexes, as well as on mouse tumor inhibition rate, spleen index, thymus index and liver index are evaluated; ASMq effect on mice under normal pressure hypoxia tolerance test and polele test are evaluated. Results: 1) Comparison between each dose group of ASMq and cyclophosphamide: The spleen/weight ratio increased significantly in each dose group of ASMq (P ＜ 0.05). The inhibition rate of each dose group of ASMq and cyclophosphamide were 49.5%、64.26%、43.43% and 50.88% respectively, in which inhibitory effect was observed. But there were no significant differences between the cyclophosphamide and ASMq high does group. Compared with tumor model group, serum IL-1β level varied in ASMq every dose group significantly (P＜0.05), the content of IL-1β was the highest in ASMq middle dose group. Compared to the group of tumor model and positive control, Serum IL-2 content increased in every dose group of ASMq significantly (P＜0.05), and ASMq middle dose group was the highest. The serum TNF-α content was higher in every dose group of ASMq than in the tumor model group and in cyclophosphamide, there was significant difference (P＜0.05), which was the highest in ASMq middle dose group. Compared with tumor model group, serum IFN-γ content varied in every dose group of ASMq significantly (P＜0.05), the content of IL-1β was the highest in ASMq middle dose group. Compared to tumor model group and cyclophosphamide, Serum IFN-γ content increases in every dose group of ASMq, there was significant difference (P＜0.05), Serum IFN-γ was the highesin in ASMq middle dose group. The serum LPO content in every dose group of ASMq were all lower than tumor model group and cyclophosphamide, there was significant difference (P＜0.05), and serum LPO was the lowest in ASMq middle dose group. Compared with tumor model group, the serum NK content varied significantly in every dose group of ASMq, there was significant difference (P＜ 0.05), the content of NK was the highest in ASMq middle dose group. In ASMq high dose group, CD3+, CD8+ and CD4+ was reduced
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apparently, the CD4+/CD8+ratio increased and The CD4+/CD8+ ratio was close to the normal value. In ASMq middle dose group, CD8+ decreased, CD3+, CD4+ CD8+ and CD4+ was reduced apparently, the CD4+/CD8+ratio increased. The CD4+/CD8+ ratio was close to the normal value. There was no significant improvement in the ASMq low dose group. Comparison between normal control and tumor model group, the SOD，GSH-PX, MDA content in the liver of both ASMq and tumor groups were significantly different. The difference was significant (P＜0.05). The SOD，GSH-PX content in the liver of ASMq every dose group increased, there was significant difference (P＜0.05), ASMq middle dose group was the highest. The MDA content in mouse liver, of every dose group of ASMq were all lower than tumor model group and cyclophosphamide group, there was significant difference (P＜ 0.05). ASMq middle dose group was the lowest. After examinations of mouse liver, spleen, thymus and other organs by HE staining methods, Pathological conditions are all shown normal, necrosis and liquefaction of transplanted S-180 tumor mouse tumor was observed. 2) The inhibition rates of 5-Fu-ASMq high dose group, 5-Fu-ASMq middle dose group, low dose group of 5-Fu +ASMq And 5-fluorouracil group were 52.26%, 66.41%, 44.87% and 48.02%. There was significant difference between normal control group and 5-Fu group in SOD, GSH-PX content in serum(P＜0.05), SOD, GSH-PX content decreased significantly in every dose group of ASMq, In ASMq middle dose group, the highest content of SOD GSH-PX was close to the normal value. The MDA value of which was lowest there was significantly reduced comparing with the 5-Fu control group, the difference was significant (P＜0.05). Compared to tumor model group with, serum SOD, GSH-PX content in the model group, both ASMq high and low dose groups were significantly lower. The difference was significant (P＜ 0.05), in which the ASMq middle dose group maximum has been close to or exceed the normal values. 3) Compared with the spleen/weight ratio each group of ASMq and cyclophosphamide, ASMq every dose group significantly increased, there was significant difference (P＜0.05), in which ASMq middle dose group. The inhibition rate of each dose ASMq and cyclophosphamide were 49.5% 、 64.26%、 43.43% and 50.88% respectively, which inhibitory effect was observed, but there has no difference between the groups of cyclophosphamide and ASMq. Compared with the serum IL-1βcontent, ASMq every dose group significantly changed, there was significant difference (P＜0.05), the content of IL-1βwas the highest in which ASMq middle dose group Compared with the serum IL-1βcontent between the group of tumor model and positive control, ASMq every dose group significantly increased, there was significant difference (P＜0.05 ), in which ASMq middle dose group was the highest, The serum TNF-αcontent in ASMq every dose group were all higher than tumor model group and cyclophosphamide, there was significant difference (P＜0.05),. in which ASMq middle dose group was the highest, Compared with the serum IFN-γ content, ASMq every dose group significantly changed, there was significant difference (P＜0.05), the content of IL-1βwas the highest in which ASMq middle dose group. Compared with the serum IFN-γ content in tumor model group and cyclophosphamide, ASMq every dose group of relative to rise, there was significant difference (P＜0.05), in which ASMq middle dose group was the highest, The serum LPO content in ASMq every dose group were all lower than tumor model group and cyclophosphamide, there was significant difference (P＜0.05), in which ASMq middle dose group was the lowest, Compared with the serum NK content, ASMq every dose group significantly changed, there was significant difference (P＜0.05), the content of NK was the highest in which ASMq middle dose group ASMq high dose group, CD3+、CD8+ and CD4+ was reduced apparently, the CD4+/CD8+ratio increased, and The CD4+/CD8+ ratio was close to the normal value. ASMq middle dose group, CD8+ decreased, CD3+、CD4+ CD8+ and CD4+ was reduced apparently, the CD4+/CD8+ratio increased, elevated CD4+/CD8+ ratio increased, and this ratio was close to the normal value. There was no
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significant improvement with the ASMq low dose group. Compared with, the SOD, GSH-PX, MDA content in the liver of both ASMq and tumor groups were significantly difference. The difference was significant (P＜0.05 ).Compared with the SOD，GSH-PX content in the liver of tumor model group, ASMq every dose group of relative to rise, there was significant difference (P＜0.05), in which ASMq middle dose group was the highest, Compared with the MDA content in mouse liver, ASMq every dose group were all lower than tumor model group and cyclophosphamide, there was significant difference (P＜ 0.05), in which ASMq middle dose group was the lowest, HE staining methods of mouse liver, spleen, thymus and other organs Pathological conditions are all normally, transplanted S-180 tumor mouse tumor necrosis and liquefaction. 4) In the 5-FU high-dose ASMq group, 5-FU medium-dose ASMq group, 5-FU low-dose ASMq group and 5-FU group, the values of tumor inhibition rate were 42.41%、58.09%, 41.58% and 50.83%, respectively, showing that tumor function had been weakened. Compared with the model group, the thymus/body weight ratio was clearly reduced in the 5-FU medium-dose ASMq group and5-FU low-dose ASMq group. Compared with the 5-FU group, the spleen/body weight ratio was clearly increased in the 5-FU high-dose ASMq group, 5-FU medium-dose ASMq group, and 5-FU low-dose ASMq group, and the difference was significant (P＜0.05). The 5-FU ASMq dose groups had reduced levels of SOD and GSH-PX compared with the 5-FU control group, and the difference was significant (P＜0.05). In the 5-FU medium-dose ASMq group, SOD and GSH-PX levels were high. With respect to MDA values, the 5-FU medium-dose ASMq group had the lowest values and in comparison with the model control group, the difference was significant (P＜0.05). With respect to SOD and GSH-PX levels, compared with the model group, the 5-FU high dose ASMq group had lower levels, and the difference was significant (P＜0.05). 5) Compared with the AF model group ,serum ALT, AST content, in every dose group of ASMq significantly decreased, there was significant difference (P＜0.05), which was the lowest in ASMq middle dose group, ALT, AST content were close to the normal value. The TP value of ASMq middle dose group is the highest, compared to the AF model control group, which had significantly increased, the difference was significant (P＜0.05). Compared with the normal control group, serum ALT, AST content in abnormal savda munziq every dose group significantly decreased, there was significant difference (P＜ 0.05), TP value of which in the ASMq middle dose group was the highest. Compared with the AF model group, SOD, GSH-PX content in the heart, significantly increased in ASMq every dose group. The difference was significant (P＜0.05), in which ASMq middle dose group had the highest content of SOD, GSH-PX and which were close to the normal values. Compared with the AF model group, MDA value of ASMq with middle doses was significantly lower; the difference was significant (P＜0.05). Compared with the normal control group SOD, GSH-PX content in the heart, ASMq high and low dose groups were significantly lower. The difference was significant (P＜0.05), in which the ASMq middle dose group maximum has been close to or exceed the normal values. ASMq cannot only improve the spleen index, but also reduce the serum levels of ALT, AST content and improve the mouse heart SOD, GSH-PX content, and protect the heart and liver in preventing necrosis. 6) Experiments of transplanted tumor S-180 mice model for climbing, hypoxia tolerance: Compared with cyclophosphamide group, ASMq high dose group, medium dose group, low dose group showed significantly higher spleen/body weight ratio; the differences were significant (P＜0.05). Compared with tumor model group, the abnormal savda munziq middle dose group and low dose group have obvious liver/body weight ratio changes, the difference was significant (P＜0.05). Compared with cyclophos- phamide group, in terms of the content of SOD, GSH-PX in serum, every dose group of abnormal Savda Munziq is increased significantly, and is close to the normal level; the difference was significant (P＜0.05). The dose of MDA in abnormal savda munziq is
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the lowest, compared with tumor model group; the difference was significant (P＜ 0.05). Compared tumor model group with normal controls, the time of climbing was decreased significantly (P＜0.05). Compared with cyclophosphamide, every dose group of abnormal savda munziq can improve the time of mice climbing, particularly in abnormal savda munziq dose for which the effect is significant (P＜0.05). Compared with tumor model group, every dose group of abnormal savda munziq can enhance the time of mice hypoxia tolerance, and there is significant difference (P＜0.05). Compared with the normal control group, every dose group of abnormal savda munziq can enhance the time of hypoxia tolerance; especially for the dose of abnormal savda munziq the effect is most remarkable. 7) Experiments of transplanted EAC tumor mice model for climbing, hypoxia tolerance test: Compared with cyclophosphamide group, the abnormal savda munziq high dose group, middle dose group, and low dose group have increased spleen/body weight ratio, the difference was significant (P ＜ 0.05). Compared with tumor model group, the abnormal savda munziq middle dose group and the low dose group have obvious liver/body weight ratio changes; the difference was significant (P ＜ 0.05). Compared the model group tumor with normal control group, the climbing time was decreased significantly (P＜0.05). Compared with cyclophosphamide group, every dose group of abnormal savda munziq can improve the mice climbing time, particularly for the dose of abnormal savda munziq the effect is significant (P＜0.05). Compared the content of SOD, GSH-PX in serum of cyclophosphamide group, every dose group of abnormal savda munziq was increased, in which the content of SOD, GSH-PX of middle dose group is close to the normal value, and cyclophosphamide group was increased significantly, the difference was significant (P＜0.05). The MDA value of abnormal savda munziq is lower than tumor model group. Compared with the normal control group, every dose of abnormal savda munziq group can enhance hypoxia tolerance in mice; especially the middle dose abnormal savda munziq is the best. Compared with tumor model, every dose group of abnormal savda munziq was increased; the difference was significant (P＜ 0.05). Compared with verapamil, the abnormal savda munziq high and low dose groups were decreased, and the difference was significant (P＜ 0.05). Conclusion: 1) This study found that ASMq can improve immune system function, enhance antioxidant enzyme activity in immune organs, and regulate the lymphocyte subsets levels and cytokines to reach the purpose of tumor suppression. Performance of experiments in transplanted S-180 and EAC tumor models in mice showed decreased mass volume, decreased weight, and necrosis and liquefaction in the tumor, by thus the anti-tumor rate increased. 2) The ASMq has a good antagonism on the toxicity side effects of the chemotherapy drug doxorubicin and 5-fluorouracil (AF), it can protect the liver and heart and reduce the damage of chemotherapeutic drugs on the liver and heart. It can reduce serumin ALT, AST content and improve the level of TP. It increases the content of the mouse model of heart tissue SOD and GSH-PX, and reduces the level of MDA. Abnormal black Munziq can improve the content of serum SOD and GSH-PX in 5-fluorouracil Reducing Toxicity Model and reduce the level of MDA. 3) The ASMq has a function of anti-oxidation, hypoxia and anti-fatigue effect in vivo. Compared with the other control group, ASMq can significantly improve the general living conditions of the tumor model mice, active state and mouse fur luster, while weight is not significantly reduced. 4) It was found that compared to ASMq high dose group and low dose group, in terms of suppressing the transplanted S-180 and EAC tumor, the protective effect of toxic side effects induced by the chemotherapy drug doxorubicin and 5-fluorouracil (AF), 5-fluorouracil efficiency attenuation, anti-oxidation, hypoxia and anti-fatigue effect in climbing the pole and hypoxia experiment, ASMq middle dose group shows better effect.
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A-104Study on the Possible Mechanisms of Xinjiang Red Raspberry Fruit Extract in Reducing Blood Pressure and Alleviating Oxidative Stress in SHR Rats
H.Jia 1, Upur Halmurat2*1Public Health Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;2Traditional
Uighur Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to [email protected]
Objectives: To evaluate antihypertension effect of Xinjiang red raspberry fruit extract on spontaneously hypertensive rats (SHR) and to study the possible mechanism from antioxidant perspective. Furthermore, to provide a scientific basis for cardiovascular protective effect of red raspberry. Methods: 1) Red raspberry fruit was extracted with 95% ethanol, defatted with petroleum ether, and partitioned with ethyl acetate and n-butanol successively. Then the ethyl acetate extracts of raspberry (EER), n-butanol extracts of raspberry (BER) and water section of raspberry (WSR): ①using Folin-Ciocalteu method to determination of total phenolic content and calculated as gallic acid equivalents (GAE); ② using DPPH and hydroxyl radical scavenging experiments to determination of antioxidant activity of different parts; ③ analysing the relationship between total phenols centent and antioxidant activity. 2) Thirty two 16-week-old male SHR rats were randomly divided into control group (SHR group), EERL group (EER 100 mg/kg·d), EERH group (EER 200 mg/kg·d) and captopril group (captopril 12.5 mg/kg·d). Another 8 age-matched male Wistar-Kyoto (WKY) rats were used as normotensive group. Systolic blood pressure was measured by non-envasive tail-cuff method. After 5 weeks of treatment, blood and tissue samples were collected for determination of concentrations of serum nitricoxide (NO), superoxidedismutase (SOD), total anti-oxidation capability (T-AOC), malondialchehyche (MDA) and plasma endothelin (ET). Using light microscopy and scanning electron microscopy to observe morphological changes of tissues. The expression of eNOS mRNA and p22phox mRNA in aortas was detected by real time PCR (RT-PCR) methods; aortic eNOS, p22phox and ET-1 protein expression was detected by immunohistochemistry. Pearson analysis and multiple regression analysis were applied to analysis the relationship of indicators. Results: 1) In different extracts, EER had the highest total phenols content (96.76 mgGAE/g) and WSR had the lowest (10.56 mgGAE/g). In antioxidant activity experiment, different extracts of raspberry showed good antioxidant activity, among them EER exhibited the highest activity, especially in OH·scavenging and better than VC and gallic acid. There is a significant association between the antioxidant potency and total phenolic content. 2) Antihypertension study showed that: ① compared with SHR group, EER group and captopril group were significantly lowered SHR rats blood pressure, declined more evident in EERH group; ② intervention groups also can increase SHR rat’s serum SOD, T-AOC and NO levels, lower MDA and ET levels, compared with SHR group, SOD levels increased more obvious in EERL and EERH group (P＜0.01); T-AOC levels increase more obvious in EERH group (P＜0.01); NO level increased more obvious in EERL (P＜0.01); MDA level decreased more obvious in captopril group and EERH group (P＜0.05), while the level of plasma ET decreased more obvious in EERH group (P＜0.05); ③ EER also can affect serum lipids of SHR in a different degrees, compared with SHR group, TC level in EERLgroup (P＜0.05) and LDL-C level in EERH group (P＜ 0.05) were decreased more obvious, while HDL-C level increased more obvious in EERL group (P＜0.05) and EERH group (P＜0.01); ④ pathomorphology results showed that compared with WKY, under light microscope, SHR rat aortic endothelial cells and smooth muscle cells
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arranged less regular, endometrial thickness increased (P＜0.01); under scanning electron microscope, SHR rats aortic endothelial cells lose their normal state, arranged loose; after intervention, aortic intima surface showed more smoother than SHR group, most of the endothelial cells arranged regular and tidy and artery intima-media thickness slightly reduced, more better in EERL group (P＜0.05); SHR rats left ventricular mass index increased as compared with WKY (P＜0.05), after intervention, left ventricular mass index in EERH group and captopril group decreased more obvious (P＜0.05); ⑤pearson analysis showed that SHR rat blood pressure negatively correlated with the serum NO, SOD and T-AOC, positively correlated with plasma ET and serum MDA level. 4) In experiments aimed at describing molecular mechanisms of this effect, the expression levels of aortic eNOS mRNA and protein were lower in SHR rats than that in WKY (P＜0.05), and both mRNA and protein expression of p22phox were higher than those in WKY (P＜0.01), but ET-1 protein expression lower in SHR rats than that in WKY (P＜0.01); compared with SHR group, EER was able to significantly increase both mRNA and protein expression of the SHR rat aort (P＜ 0.01), lowered both mRNA and protein expression of p22phox (P＜0.01) and ET-1 protein expression decreased more in EERL group (P＜0.05); pearson analysis showed that SHR rat blood pressure negatively correlated with the eNOS mRNA and protein expression and positively correlated with the aortic p22phox mRNA and protein expression and ET protein expression; p22phox mRNA also negatively correlated with serum SOD activity and T-AOC; Multiple regression analysis showed that p22phox mRNA, eNOS mRNA, serum SOD activity and ET were the main factors that affect blood pressure. Conclusions: 1) The extracts of raspberry shows high scavenging effects on the DPPH and hydroxyl radical, among them EER exhibits the highest activity and shows significant correlation between the total phenolic contents and the antioxidant activity; 2) EER has the function of reducing SHR rats blood pressure, serum lipids and improve the morphology of aortic and cardiac anomalies; 3) The effect of red raspberry extract in lowering blood pressure of SHR rats may be related to its antioxidant effect through increasing serum NO, SOD activity and T-AOC levels, and decreasing plasma ET and MDA levels. This effect may be related to the activation of NO-NOS system by scavenging free radicals, so as to improve vascular endothelial dysfunction and help to lower blood pressure. The molecular mechanisms of this effect may be related the down regulating expression of p22phox and ET-1 and increasing SOD activity and T-AOC, and up-regulating eNOS expression, which in turn increases NO generation and improves endothelial dysfunction. The specific cell signaling mechanisms of this effect are still unknown, and further studies are required to address these. 4) The results of this study confirmed that polyphenols rich raspberry have potential cardiovascular protective effects and further provide more clues to the development and utilization of Xinjiang raspberry resources and research and development antihypertensive natural health products and medicines.
A-105The Abnormal Savda Syndrome CD41, CD62p and fibrinogen clinical analysis of change
Cao Xiaobin1, Halmurat Upur2*1 Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uighur Autonomous Region,
China;2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
Objective：Assessment and analysis of different age exception abnormal savda syndrome to patients
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with Platelet Activation molecular α-particles membrane protein (CD62p), Integringene familyⅡ b/Ⅲ a Integrin (CD41), Fibrinogen relationship. Methods：Use of monoclonal antibodies and flow cytometry technology, research and analysis of different age groups (elderly aged ≥60 year-old, middle age groups age 45-59 year-old age group, youth <45year-old) with the exception of abnormal savda syndrome of platelet activating molecules CD41, CD62p and fib expression and correlation. Results：Abnormal savda syndrome of elderly group patients with CD62p, CD41 and fibrinogen were significantly higher than other abnormal savda syndrome age patient groups (P<0.01); Abnormal savda syndrome in different age groups of patients CD41, CD62p and fibrinogen were higher than other age groups of abnormal savda syndrome CD41, CD62p and fibrinogen (P<0.05).The increase level of growth and age signifcantly correlated (P<0.01). Conclusion：① The old age patients with abnormal savda syndrome was in the majority. ②Abnormal savda syndrome in older age-group patients with blood CD41, CD62p and Fibrinogen more noticeable changes. ③ For the elderly age-group patients of abnormal savda syndrome，more attention should be concerned about a variety of platelet activating factor stimulation in vivo platelet activation, but also to early warning, prediction, prevention of abnormal savda syndrome to elderly patients and the incidence of thrombosis. With age in patients with Abnormal Savda Syndrome in platelet activating CD62p, CD41 and fibrinogen significantly increased platelet adhesion protein CD41, CD62p expression and release, is active. The increase in the degree of age and abnormal growth of the gall bladder syndrome were positively correlated. Abnormal savda mechanism and prognosis has an important role in clinical applications and research.
A-106The clinical report of Awrixim syrup to treat the Vasculitis patients,one case
G.Abduriyim 1, P.Ablimit 1, R.Jappar 1
1The Uighur Medical Hospital of Turpan ,838000，Turpan, Xinjiang Uighur Autonomous Region ，China
The Traditional Uygur Medicine herbal formula, Awrixim syrup is composed of seven herbal ingredients,. It has long been used as a decoction in Traditional Uygur Medicines for cardiovascular disease and neuro sysytem diseases. According to the Traditional Uygur Medicines theory Awrixim syrup was described to treat the diseases through eliminating the Abnormal Savda Hilit ( dry and cold)， improve blood circulation, benefit to brain, heart and liver. Awrixim syrup is recorded in State Pharmacopoeia of People’s Republic of China in the Uygur Medicine volume. We used the Awrixim syrup to treat vasculitis patients，each 30ml, three times a day for a total of three months. Awrixim syrup siginificantly improved the symptom of patients.
A-107The Study of Basic Exploration and Application of Uighur Medicine Xipayi Mouth Rinse in Treatment of Gingivitis
Habiba1, S.Nelski 2, Upur Halmurat3*1 First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China; 2Heidelberger Traditional Chinese medicine
Physiccal therapy Institute, Zwingenberg,Germany;3Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
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Objective: ① Studying the role of NF-κBp65 and COX-2 in the pathogenesis of gingivitis and the effect of Xipayi mouth rinse on gingivitis at the mRNA level. ②Observing the effect of Xipayi mouth rinse on different concentrations on the activities of interleukin-6 (IL-6) from human gingival fibroblast (HGF) induced by lipopolysaccharide (LPS) and the effect of Xipayi mouth rinse on the DNA synthesis and the changes of cell cycle of human gingival fibroblast (HGF) inhibited by lipopolysaccharide (LPS). ③ Investigating the effects of Xipayi mouth rinse on the control of dental plaque in cases with gingivitis. Methods: ① Wistar rats were randomly divided into 3 groups: normal group, gingivitis model group and Xipayi mouth rinse group, and experimental gingivitis model was established in the last 2 groups by means of suturing the neck of right upper second molar of rats, high –carbohydrate diet and P.gingivalis inoculation, Wistar rats in Xipayi mouth rinse group treated with Xipayi mouth rinse while gingivitis model group treated with 0.9% sodium chloride, the expression of NF-κBp65 and COX-2 mRNA in gingival tissue of 3 groups were examined by RT-PCR. ② HGF were stimulated with LPS at the concentration of 25ug/ml, radioimmunoassay (RIA) was used to examine the effect of Xipayi mouth rinse at the concentrations of 12.5-200 ug/ml on the secretion of IL-6 in the supernatant of the cell culture; Flow cytometry was used to examine the effect of Xipayi mouth rinse at the concentration of 50μg/mL on the DNA synthesis and changes of HGF cell cycle. ③Patients with gingivitis were randomly divided into therapy group and control group. The Xipayi mouth rinse in therapy group and 0.9% sodium chloride in control group were used by patients for 7days and 5 times each day, plaque index(PLI), gingival index(GI), sulcus bleeding index(SBI) and other clinical indexes were measured before and after treatment. Subgingival plaque sample were collected and stained by congo red solution. A total of 200 bacteria were counted including spirochaeta, coccus, bacillus, fusiformis and campylobacter. Compare the clinical and microorganism indexes between two groups. Results: ① Gingivitis model established successfully in gingivitis model group, compared with the normal group, the mRNA expression of NF-κBp65 and COX-2 increased (P<0.05) in gingivitis model group, while the expression of NF-κBp65 and COX-2 decreased (P<0.01) in Xipayi mouth rinse group. The expression of NF-κBp65 had a relation to COX-2 in gingivitis model group (P<0.05). ② IL-6 secreted by HGF was significantly inhibited by Xipayi mouth rinse in a dose-dependent manner, the inhibitory effect of Xipayi mouth rinse at the least concentration is approximately equal to the effect of dexamethasone; the percentage of HGF in G1 phase during exponential growth was increased by LPS, while the percentage of HGF in S phase was decreased as well as proliferation index (P<0.01). Xipayi mouth rinse ameliorated this phenomenon. ③ PLI, SBI , GI and other clinical indexes in therapy group were improved significantly (P<0.01)after seven- day washing, as well as percentage of spirochaeta, bacillus in dental plaque were decreased and of coccus was increased (P<0.01, P<0.05, P<0.01). In addition, the effectiveness of treatment in experimental group was significantly better than that in control group (P<0.01). Conclusion: ①The expression of NF-κBp65 related to COX-2 may take part in the pathogenesis of gingivitis; Xipayi mouth rinse can inhibit the secretion of pro-inflammatory cell factor IL-6 from HGF induced by LPS. Xipayi mouth rinse may take role in the treatment of gingivitis in this way. ②Xipayi mouth rinse can significantly ameliorate the inhibitory effect of LPS on the prolifiration of HGF, thus suggesting the gingival tissue regeneration effect of Xipayi mouth rinse in treatment and prevention of gingivitis. ③Xipayi mouth rinse can control dental plaque and relieve gingivitis effectively. ④This study give us its scientific bases to be used in clinic widely.
A-108Study on the anti-inflammatory and anti-asthmatic mechanism of Hyssopus officinalis.L active fractions
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A.Nurahmat 1, N.Rozi 1, A.Rozi 1, A.Yusup 1
1Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
Objective: To determine the extraction process of the active site of Uighur Medicine Hyssopus officinalis.L. To observe its anti-inflammatory and anti-asthmatic effect and its impact on asthma inflammatory cells, factor in rats, and investigate the mechanism of anti-asthma and its material basis for its clinical applications scientific basis. Methods: （ 1 ） Through mouse anti-inflammatory，cough，expectorant experiment to observe the anti-inflammatory, cough expectorant effects of Hyssopus officinalis.L. water，ethanol extract and the ethyl acetate extract parts.（2）The asthmatic rats model was preparated by OVA-sensitized and repeatedly spray； the lung tissue pathological changes were observed by HE staining； Count of each group in BALF in leukocyte classification and EOS number；the rats serum and BALF ET-1 levels were detected by enzyme-linked immunosorbent assay（ELISA）；the rat serum ET-1，IL-2，IL-6，TNF- levels were determinedɑ by radioimmunoassay.（3）Using the Rutin standards establish Hyssopus officinalis.L total flavonoids determination method ； The Hyssopus officinalis.L optimum extraction was selected with the L9（34）orthogonal design； the AB-8 resin optimum purification process for the total flavonoids of Hyssopus officinalis.L was selected with the L9（ 34） orthogonal design； through mouse anti-inflammatory，cough，expectorant experiment to observe the Hyssopus officinalis.L total flavonoids anti-inflammatory，cough expectorant effect；through the rat on the basis of the asthma model，the lung tissue pathological changes were observed by HE staining； count leukocyte classification and EOS number of rats in each group in the BALF；the TGF- β1 levels of rat lung tissue and BALF were detected by using ELISA.（4）Respectively，using steam distillation and SFE-CO2 extraction method to extract Hyssopus officinalis.L essential oil and its GC-MS analysis； the Hyssopus officinalis.L essential oil supercritical optimum extraction process was determined with the L9（34）orthogonal design； through mouse anti-inflammatory， cough， expectorant experiment to observe the anti-inflammatory， cough expectorant effects of Hyssopus officinalis.L. essential oil； the lung tissue pathological changes were observed by HE staining；count leukocyte classification and EOS number of rats in each group in the BALF；the TGF- β1 levels of rat lung tissue and BALF were detected by Using ELISA；Results:（1）Compared with model group，Hyssopus officinalis.L. water，ethanol extract and the ethyl acetate extract had anti-inflammatory ， cough ， expectorant effect （ P <0.05） and the role of the ethyl acetate extract more significant.（ 2） Compared with model group，Hyssopus officinalis.L.ethyl acetate dose group rats BALFLy、Eos、Neu number and serum ET-1， IL-2， IL-6， TNF- levels were significantly decreasedɑ ， the difference was statistically significant（P <0.05） . By comparison between each dose group， the above indicators in BALF of serum differences were statistically significant（P <0.05），and it had dose dependent trend. High and middle dose groups to improve lung tissue inflammation. （ 3） The maximum absorption wavelength was 507nm， it was using the colorimetric method. The standard curve equation for Y= 12.06x - 0.003 ， R² = 0.9999 ， had good linear relationship ； The determination method stability（ RSD = 0.71%）， a high accuracy（ RSD = 1.63%）； in accordance with the determination of methods for the determination of Hyssop The medicinal content of total flavonoid of Hyssopus officinalis.L. was 2.68%，The content of total flavonoid of ethyl acetate was 46.5%；The optimum extraction conditions were the eight volume times ethanol（70%），extract three times per hour； The AB-8 resin optimum separation and purification conditions were sample concentration 15mg/mL， 70% ethanol， flow rate 10mL/min， and the content total flavonoid was 66.7%；Compared with model group ， the total flavonoids of Hyssopus officinalis.L had anti-inflammatory，cough，expectorant effect（P <0.05）；Compared with model group，Hyssopus officinalis.L. total flavonoids dose group rats BALF Ly, Eos, Neu number and BALF, lung tissue TGF- β1 levels were significantly decreased，the difference was statistically significant（P <0.05） and it had dose dependent trend. Each dose groups were to improve lung tissue inflammation；（ 4） The Hyssopus officinalis.L essential oil was extracted by steam distillation and its content was 0.68%. There were detected 65 compounds by GC-MS ， in which higher levels of linoleic acid（13.83%），palmitic acid（13.80%）（+） - pulegone（8.31%），（+） the Spartak alcohol（ 6.27%）， 20 alkyl（ 5.34%）， lemongrass acid（ 4.07%）； The SFE optimum conditions were 20Pa pressure，35℃， flow 2L/min，extract 1.5h，75%extractioncarry（ethanol: medicinal ratio = 75ml: 100g），and its content was 5.5%. There were detected 34 compounds by
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GC-MS in which higher levels of the γ-Valley sterol（31.882%），21 alkyl（19.953%）， linolenic acid（ 16.279%）， hexadecane（ 15.939%）， palmitic acid（ 13.80 %）， higher content of linolenic acid ethyl ester（8.766%）；Compared with model group， the essential oil of Hyssopus officinalis.L had anti-inflammatory，cough，expectorant effect（P <0.05）；Compared with model group ， Hyssopus officinalis.L. essential oil dose group BALF Ly 、 Eos 、 Neu number and BALF、 lung tissue TGF- β1 levels were significantly decreased， the difference was statistically significant（P <0.05） . and it had dose dependent trend. Each dose groups were to improve lung tissue inflammation. Conclusion: The Hyssopus officinalis.L water ， alcohol ， ethyl acetate extract ， total flavonoids ， essential oil all had significant effect of anti-inflammatory，cough，phlegm，and asthma. The role of Hyssopus officinalis.L ethyl acetate extract was in relation with inhibition the level of Eos，ET-1， IL-2， IL-6，TNF- .The role of Hyssopusɑ officinalis.L total flavonoids and essential oil was in relation with inhibition the level of Eos，TFG- β1. Speculated that the total flavonoids and essential oil of Hyssopus officinalis.L were the material basis of its anti-inflammatory and anti-asthmatic effect.
A-109A study of the effects of the leaf extract of Cydonia Oblonga Miller on blood hemostasis, coagulation and fibrinolysis in mice
Anwar Umar1,2, Zhou Wenting1, Ibadet Tohti1, A.Abliz 1, Bernard Bégaud 2, Nicholas Moore 1,2*
1Department of pharmacology , Medicine, Xinjiang Medical University, Xinjiang 830011, China; 2Department of Pharmacology,
Universit´e de Bordeaux, 33076 Bordeaux, France; Correspondence should be addressed to Nicholas Moore,
Objective: To study the anti-thrombotic effect of Cydonia Oblonga Miller (COM) leaf extracts, which are used for cardiovascular prevention in Traditional Uighur Medicine. Methods: The effects of three doses of COM (20, 40, 80 mg/kg/day), aspirin (5 mg/kg/day) and gingko biloba extracts (20 mg/kg/day) in mice were compared to untreated controls. Products were given orally for 14 days before the experiments. Bleeding time and clotting time were measured in mice using the tail cutting method and glass slide method, respectively. The death rates in pulmonary thrombosis induced by collagen-epinephrine, thrombolysis in vitro and euglobulin lysis time (ELT) were also measured. Results: Low, medium and high doses of COM leaf extracts dose-dependently prolonged the bleeding time (by 2.17, 2.78 and 3.63 times, respectively, vs. 2.58 for aspirin) and the clotting time (by 1.44, 2.47 and 2.48 times compared to controls, vs. aspirin 1.91 times), and reduced the mortality rates in the pulmonary embolus model (by 27, 40 and 53%, respectively, compared to 47% for aspirin). All three doses significantly and dose-dependently increased spontaneous thrombolysis (by 45, 55 and 63%, respectively, compared to 56% for aspirin) and shortened ELT to 71, 61 and 43% of controls (p<0.01), vs. 43% for aspirin. Conclusion: the effects of COM on hemostasis, coagulation and fibrinolysis warrant further exploration.
A-110About the exploration of Bloodletting therapy of Uighur Medicine
Amier Alye1, Tuersunjian Tohsun1
1Aksu Prefecture Uighur Medicine Hospital, 843000, Xinjiang Uighur Autonomous Region, China
Bloodletting (or blood-letting) is the withdrawal of often little quantities of blood from a patient to cure or prevent illness and disease. Bloodletting was based on an ancient system of medicine in which blood and other bodily fluid were considered to be "humors" the proper balance of which maintained health. It
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was the most common medical practice performed by Uighur Medicine doctors from antiquity up to the late 19th century, a time span of almost 2,000 years. The practice has now been abandoned for all except a few specific conditions. It is conceivable that historically, in the absence of other treatments for hypertension, bloodletting could sometimes have had a beneficial effect in temporarily reducing blood pressure by a reduction in blood volume. However, since hypertension is very often asymptomatic and thus undiagnosable without modern methods, this effect was unintentional. In the overwhelming majority of cases, the historical use of bloodletting was harmful to patients. This article describes the history of bloodletting therapy, the presently application situation, clinical significance, the operation method and the matters needing attention, and shows the characteristics of the traditional treatment to improve the therapeutic significance.
A-111Analysis of intestinal bacterial communities in four group abnormal hilit hyperpietics and normal hilit of Uighur nationality
Jiang Wei1, Halmurat Upur2, Dilxati Yimit1*, Ma Xiumin 1
1Department of Pathogenic Biology, Xinjiang Medical University, Urumqi 830011, China; 2 Traditional Uighur Medicine Institute,
Xinjiang Medical University, Urumqi 830011, China; *Correspondence should be addressed to [email protected]
Objective: The microbial diversity and differences of four abnormal hilit hyperpietics and normal hilit group was investigated using nested PCR–denaturing gradient gel electrophoresis. Methods: The total DNA in the fecal speciments of abnormal Sawda hilit and normal Sawda hilit healthy people, abnormal Khan hilit and normal Khan healthy people (each of these hilit includes 20 samples), abnormal Sapra hilit and normal Sapra hilit healthy people, abnormal Balgham hilit and normal Balgham hilit healthy people (each of these hilit includes 10 samples), total 120 subjects aged 35 to 45 years old Uighur nationality were used as templates. After the 16SrDNA genes (V6-V8 region) were amplified by using the universal primers and analyzed by DGGE. Several bands in DGGE profile specifically existed in two group lanes were excised, cloned, sequenced and searched in BLAST to find the closest relatives. Each band of DGGE and the similarity of UPGMA cluster were analyzed by Quantity One4.4.0, and the diversity, richness and evenness of intestinal microflora of bacterial diversity were analyzed by Microsoft Excel and SPSS 17.0. Construct phylogenetic trees of intestinal flora by MEGA 5.05 software. Results: (1) The results from UPGAMA cluster showed that the similarity of total intestinal tract bacteria. Abnormal Sawda hilit hyperpietics(45%-87%)＞abnormal Khan hilit hyperpietics (50-85%)＞abnormal Balgham hilit hyperpietics(42%-86%)＞abnormal Sapra hilit hyperpietics (54-82%), normal Sawda hilit (40%-80%)＞ normal Khan hilit (49%-79%)＞ normal Balgham hilit (43%-72%)＞ normal Sapra hilit (52%-75%). (2) By analyzing the diversity, richness and evenness of four groups of total intestinal bacteria, abnormal Khan hilit hyperpietics and normal Khan hilit, abnormal Balgham hilit hyperpietics and normal Balgham hilit was statistically significant (P＜ 0.05). Abnormal Sawda hilit hyperpietics respectively compared with abnormal Khan hilit hyperpietics, abnormal Balgham hilit hyperpietics was statistically significant (P＜0.05). Compared with abnormal Sapra hilit, the richness and diversity of total intestinal bacteria was statistically significant (P＜0.05), but the evenness was not significant (P＞0.05). (3) The sequences that frequency can represent each sample were compared with sequences in the Genebank nucleotide sequence database by using Blast software and phylogenetic trees were constructed. Conclusions:(1)There were some differences of similarity, species and distribution
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diversity in intestinal microbial communities of four abnormal hilit hyperpietics and normal hilit group.(2)The phylogenetic trees of intestinal flora diversity of four abnormal hilit hyperpietics and normal hilit group in Uighur nationality and the phylogenetic trees show that the existing of Firmicutes, Bacteroidetes, Verrucomicrobia, Butyrate-producing bacteriumand and Uncultured bacterium were some factors that can be determinant for the form and development of abnormal hilit hypertension.
A-112Ancient Uighur drug treatment of coronary heart disease medication compatibility law
Mamatyili Rumat 1, Aniwar Talip 1, Abduwar Abulak 1, Axim Turgun 1
1Xinjiang Uighur Autonomous Region Uighur Medicine Hospital, 830049, Xinjiang, China
Objective: Analysis of the traditional medicine laws concerning the coronary heart disease to find the characteristics of its mechanism and treatment. Methods: Collecting, systemizing and summarizing the effective prescriptions which is used to treat the symptoms of palpitation, cardiodynia, chest pain and choking sensation in chest. And then we use the Microsoft Excel to build material data tables, We classified the medicine by the data of screening function and analyse our frequency. Results: According to the using frequency arrangement, the cardiotonic drug rowed in the former, next is abnormal body fluids mature medicine, the cerebral tonic pill and the tonifying kidney and fighting the bowel medicine. The medicines including bugloss, dracocephalum moldavica, sandalwood, silkworm cocoon and roseleaf have a higher frequency. Conclusion: It has Initially formed a basic law for coronary heart disease compatibility of formula which has given priority to cardiotonic drug and wrestled with the abnormal body fluids mature medicine, the cerebral tonic pill, the tonifying kidney pill, the fighting bowel medicine and the enhancing natural force medicine.
A-113Anti-carcinogenic effects of th e poly phenol of Abnormal Savda Munzi q in association with its cytotoxicity, apoptosis-inducing properties and telomerase activity in human cervical cancer cells (SiHa)
G.Abliz 1, Ahmatjan.Mamat 2, W.Xinling 1, L.Hua 1, G.Dongmei 1, N.Amat 3*, Halmurat Upur3*
1Fifth Department of Gynecology , Affiliated Tumor Hospital , Xinjiang Medical University, 830011, Urumqi , Xinjiang Uighur
Autonomous Region, China; 2Second Department of Gynecology , Affiliated Tumor Hospital , Xinjiang Medical University,
830011, Urumqi , Xinjiang Uighur Autonomous Region, China; 3Traditional Uighur Medicine Department, Xinjiang Medical
University, 393 Medical University Road, 830011, Urumqi , Xinjiang Uighur Autonomous Region, China; *Correspondence
should be addressed to [email protected] or [email protected]
Objective: Abnormal Savda Munziq (ASMq) is a herbal preparation used in Traditional Uighur Medicine for the treatment and prevention of diabetes, cardiovascular diseases, chronic asthma and cancer. The polyphenol is main compounds contains in ASMq preparation responsible for the anticancer effect of ASMq. Methods: In this study method, including Real-time quantitative Polymerase Chain Reaction (RT-PCR) assay, MTT assay and flow cytometry were used to investigate the effect of polyphenol of ASMqp on cell viability and the potential of the polyphenol of ASMq to induce apoptosis in human cervical cancer cells SiHa and its effects on telomerase activity were investigated. Cellular
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morphological change was observed by phase contrast microscopy. Results: The MTT cell viability data revealed that treatment with polyphenol at 75~175μg/ml significantly inhibited the viability and proliferation of cells, and these effects occurred in a concentration-dependent manner and time dependent manner (P<0.01). The polyphenol can induce apoptosis of the SiHa cells, can increase the apoptosis rate in a concentration-dependent manner and time dependent manner (P<0.01). The growth inhibition and apoptosis induction by polyphenol treatment in SiHa cells was associated with the down-regulation of anti-apoptotic Bcl-2 expression and telomerase (P<0.05) and Survivin expression. In addition, polyphenol exerted a dose-dependent induction of FHIT expression. Conclusion: Together, these results suggest that polyphenol may have anti-tumor effects in human cervical cancer through cytotoxicity, apoptosis-inducing properties and telomerase activity.
A-114Cognitive enhancement and neuroprotective effects of Abnormal Savda Munziq, a formulation of Traditional Uygur Medicine, in a rat model of Alzheimer’s disease
Palidan Wushuer1, Nurmuhammat Amat2*, Halmurat Upur2*1 Neurology Department of Xinjiang Medical University affiliation Traditional Chinese Medicine Hospital, 116 Huanghe Road,
830000, PR China;2Traditional Uighur Medicine Institute of Xinjiang Medical University, 393 Medical University Road,
830011,PR China;* Correspondence should be addressed to Halmurat.Upur, Nurmuhammat.Amat [email protected] or
Objective: Abnormal Savda Munziq（ASMq），a herbal preparation in Traditional Uygur Medicine as
a treatment for age relating diseases including Alzheimer’s disease. Therefore, the potential of this preparation to protect against Alzheimer’s disease has been raised but less supported document is available. Methods: To determine the effect of ASMq on cognitive function in animal model of Alzheimer’s disease induced by Aβ1-42 in rat hippocampus. Wistar rats were orally given the ASMq at doses of 2, 4 and 8g/kg BW via feeding needle for a period of 2 weeks after 1 week after the Aβ1-42 injected to rat hippocampus. Rats were tested for spatial memory using Morris water maze test and the neurons was determined using histological techniques. Results: ASMq improved the latency time (p<0.01), pass the number of effective area (p<0.01) in Morris water maze test. Moreover, the reduction of neurons densities were also mitigated. Conclusion: These findings suggest that ASMq is a potential cognitive enhancer and neuroprotectant against Alzheimer’s disease.
A-115Composition of the essential oil and antioxidant activity of several extracts of Moluccella laevis L. (Lamiaceae)
Güvenç, A1., Özek, G2., Hürkul, M.M1., Özek, T2., Başer, K.H.C3.1Ankara University, Faculty of Pharmacy, Department of Pharmaceutical Botany, 06100, Tandoğan, Ankara,
Turkey;2Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, Eskişehir, 26470, Turkey; 3 Department of
Botany and Microbiology, King Saud University, College of Science, 11451 Riyadh, Saudi Arabia
Objective: Moluccella laevis is distributed in South Anatolia and it is İr.-Tur. element (Mill, 1982). The present study was designed to define the chemical composition of the essential oil and antioxidant
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activity of the several polarity extracts of M. spinosa. Methods: The essential oil obtained from hydrodistilation of M. laevis was analyzed by gas chromatography and gas chromatography/mass spectremetry (GC/MS), simultaneously. Results: Compounds representing 90.1% of this oil were identified. The main componenet of the essential oil was found as cis-chrysanthenyl acetate (6.6 %), hexahydrofarnesyl acetone (2 %) and fatty acids as a tetradecanoic acid (4.3 %), hexadecanoic acid (45.2 %). TBA (thiobarbituric acid) test, qualitative and quantitative DPPH (2,2-diphenyl-1 picrylhydrazyl) tests were used to evaluate the antioxidant activity of the extracts and essential oil. Total phenolic contents of the BuOH extracts were found to 150 ± 4,83 mg/g in dry weight expressed as gallic acid equivalents (GAE). All extracts showed a slightly antioxidant activity with the DPPH• test. The highest antioxidant activity was observed with the water extract in the TBA test of this plant. Conclusion: This investigation is the first report on the M. laevis.
A-116Composition of the essential oil and antioxidant activity of several extracts of Moluccella spinosa L. (Lamiaceae)
Güvenç, A1., Özek, G2., Hürkul, M.M1., Özek, T2., Başer, K.H.C3.1Ankara University, Faculty of Pharmacy, 06100, Tandoğan, Ankara, Turkey; 2 Department of Pharmacognosy, Faculty of
Pharmacy, Anadolu University, Eskişehir, 26470, Turkey; 3 Department of Botany and Microbiology, King Saud University,
College of Science, 11451 Riyadh, Saudi Arabia
Moluccella L. (Lamiaceae) of annual plants. Leaves are palmately crenate or incised. Calyx is large, with obliquely campanulate tube, membranous or rigid; corolla is bilabiate, upper lip hooded, hairy, lower 3-lobed. Genus grows mainly in the Mediterranean region and two species are native to Turkey. These species M. laevis L. and M. spinosa L. Moluccella spinosa is distributed in South Anatolia and it is rare (Mill, 1982). The present study was designed to define the chemical composition of the essential oil and antioxidant activity of the several polarity extracts of M. spinosa. The essential oil obtained from hydrodistilation of M. spinosa was analyzed by gas chromatography and gas chromatography/mass spectremetry (GC/MS), simultaneously. Compounds representing 94.7% of this oil were identified. The main componenet of the essential oil was found as hexahydrofarnesyl acetone (4%) and fatty acids as a tetradecanoic acid (8%), hexadecanoic acid (41.3%). The phenolic contents of the samples were determined using Folin-Ciocalteu's phenol reagent. TBA (thiobarbituric acid) test, qualitative and quantitative DPPH (2,2-diphenyl-1 picrylhydrazyl) tests were used to evaluate the antioxidant activity of the extracts and essential oil. All extracts showed a slightly antioxidant activity with the DPPH• test. The antioxidant activity was not observed all extract and essential oil of plant. This investigation is the first report on the M. spinosa.
A-117Determination peptides compositions of Chickpea Sprouts by high performances capillary electrophoresis
A.·Yili 1,2 , Ma.Q. L. 1,2，Zhang JunPing 1,2, Gao.Y. H1, Zhao.B 1,2, D. Jun 1, H. A.·Aisa 1,2,*1 Xinjiang Technical Institute of Physics and Chemistry, Academy of Sciences of the People's Republic of China, Urumqi,
Xinjiang, PRC, 830011, Beijing South Road 40-2, fax (+86-991) 3835679,China; 2 Key Laboratory of Xinjiang Indigenous
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Medicinal Plants Resource Utilization,China
High Performances Capillary Electrophoresis (CE) is known as an efficient and rapid separation technique with high resolution, which is frequently employed in the separation of biological macro molecular such as proteins [1], peptides [2] and nucleic acids [3].In our continuation of research on peptides from chickpea sprout [4], herein we report preparation of basic peptides fraction of chickpea sprouts[5] as well as quantify the amount of peptides and determine the purity of a peptides extracts by High Performances Capillary Electrophoresis and evaluation antioxidant activity in vitro.
CE Chromatography for Standard proteins and sample was as follow:
c Minutes0 5 10 15 20 25 30
AU
-0.01
0.00
0.01
0.02
0.03
AU
-0.01
0.00
0.01
0.02
0.0310
kD
20kD
35kD
50
kD
100k
D
150k
D
(225
kD)
PDA - 220nmName
Minutes
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16
AU
0.00
0.01
0.02
0.03
0.04
0.05
AU
0.00
0.01
0.02
0.03
0.04
0.05
8020
.969
083
30.4
730
8673
.606
093
72.0
610
9803
.826
099
60.2
810
1052
5.04
4011
117.
2990
1170
7.87
4012
676.
6040
1830
2.79
80
3180
6.12
50
PDA - 220nmyzdb
Quali ty
Fig.1. CE Chromatogram was developed by PA 800 Enhanced Systems and the PA 800plus Pharmaceutical Analysis Systems. CE Chromatogram A, Protein Size Standard, Peak identities: 10, 20, 35, 50, 100, 150, and 225 kDa proteins. CE Chromatogram B. Basic peptides fraction of Chickpea Sprouts; Our results indicated that basic total peptides fraction of chickpea sprout was composed by at least 7 peptides which molecular weights range from 7KDa to 12.8KDa, among them two peptides that appeared at 9.7min and 10.25 min representative to major peptides compositions of this fraction; Although we have isolated two individual bioactive peptides from this fraction and determined it part of the primary structure in previous works, this result indicated that but there are others peptides still to be need isolated for full identification peptides material basis of traditional medicine of Chickpea.Capillary electrophoresis (CE) has become an effective replacement for manual slab gel electrophoresis processes due to its automation, quantization, fast speed, and high efficiency. Based on this, we developed a method for the analysis of the peptides compositions of plants extracts by High Performances Capillary Electrophoresis. This method has the advantage of speed, sensitivity, and reproducibility and could be applied for quality control of the medicinal plants on the basis of their peptides compositions.
A-118Effect of Cydonia Oblonga on experimental Thrombosis in rats
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A
B
Zhou Wenting1, Anwar Umar1,2*, Wuliya Yimin1, A.Abliz 1, I.Tohti 1, Bégaud Bernard2, Moore Nicholas1,2*
1Department of Parmacology, Xinjiang Medical University, Xinjiang 830011, China; 2Department of Pharmacology, Universit´e
de Bordeaux, 33076 Bordeaux, France; *Correspondence should be addressed to Nicholas Moore,
Objective: Cydonia Oblonga Miller. (COM) leaf is used in traditional Uyghur medicine for prevention of cardiovascular disease. This study is to investigate the effects of COM as an antithrombotic agent. Methods: Two models of experimental thrombosis: common carotid artery FeCl3-induced thrombus formation and inferior vena cava thrombosis occlusion time were used to evaluate the effects of COM on arterial and venous thrombosis in rats. Low 20mg/kg, medium 40mg/kg, high 80mg/kg doses of COM aqueous extracts were compared with the model group, aspirin 5mg/kg and Ginkgo 20 mg/kg. The plasma concentrations of thromboxane B2 (TXB2) and 6-keto-prostaglandine F1 alpha (6-keto-PGF1) were measured. Results: The venous occlusion time (OT) was prolonged and arterial and venous thrombus weights were dose-dependently reduced in COM-treated groups compared with the model group (p<0.01). Aspirin and gingko had effects similar to the effects of the medium dose of COM.. TXB2 concentrations were dose-dependently decreased and the concentrations of 6-keto-PGF 1 increased in all COM-treated groups and aspirin-treated group compared with the model group (p<0.05). Conclusion: There was a clear association between 6-keto-PGF 1/TXB2 concentration ratios and arterial or venous thrombus weight for all products, and for occlusion time with COM but not aspirin or gingko. COM confirmed in vivo antithrombotic effects that may be related to its traditional use in Uygur Medicine to prevent cardiovascular disease.
A-119Effects of Ocimum Basilicum L. extracts on Lipid Metabolism and Oxidant Status in Hyperlipidemic rats
Anwar Umar1,2, Zhou Wenting1, Wuliya Yimin1, Guzalnur Imam 1, A.Hasan 1, I.Tohti1*, B.Berké2, Bernard Bégaud 2, Nicholas Moore 1,2*
1Department of Pharmacology，Xinjiang Medical University, Xinjiang 830011, China; 2Department of Pharmacology, Universit´e de Bordeaux, 33076 Bordeaux, France; Correspondence should be addressed to Nicholas Moore,
Objective: Ocimum Basilicum L (OBL) has effets on blood pressure and platelet function. To test a possible effect on lipid metabolism, we investigated the effects of OBL in rats fed with a high-fat emulsion. Methods: Male Wistar rats fed high-fat emulsion by gavage were treated with aqueous (A-) and ethanol (E-) OBL extracts 100, 200 or 400 mg/kg or lovastatin 2 mg/kg, for 28 days. The animals were then tested for serum and hepatic total cholesterol (TC), triglycerides (TG), high density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL), liver malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and hepatic index (liver weight/body weight), and whole blood viscosity and hematocrit. Results: Compared to controls, A-OBL (400mg/kg) decreased TC and LDL and increased HDL (p<0.01); E-OBL (200 and 400mg/kg) lowered TC, TG and LDL levels and increased HDL (p<0.01); A-OBL (200 and 400mg/kg) decreased liver TC and TG concentrations (p <0.05). All three doses of E-OBL decreased liver TC and TG (p <0.05). The hepatic index was decreased by A-OBL 200mg/kg and by E-OBL 100mg/kg. High-fat emulsion diet increased plasma and
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liver MDA and decreased SOD and GSH-PX. OBL extracts, especially E-OBL, decreased MDA and increased SOD and GSH-Px. We also observed the improvement of morphology indexes of OBL-treated rats. Conclusion: The aqueous and ethanol extracts of OBL may improve lipid profile and antioxidant status of rats fed with high-fat emulsion. In addition to its other properties, OBL might be useful in the prevention and treatment of atherosclerosis.
A-120Effects of soybean peptide on neuroendocrine immune network, memory, hematology and plasma biochemistry in young healthy volunteers
Nurmuhammat Amat1*, Palidan Wushuer2, Guzalnur Imam1, Dilxat Yimit 1, Nobuo Yamaguqi 3
1Traditional Uighur Medicine Department of Xinjiang Medical University, 393 Medical University Road, 830011, PR China; 2Neurology Department of Xinjiang Medical University affiliation Traditional Chinese Medicine Hospital, 116 Huanghe Road,
830000, PR China; 3Ishikawa Natural Medicinal Products Research Center, Fukubatake, Kanazawa, Ishikawa, Japan,
*Correspondence should be addressed to Nurmuhamamt Amat, [email protected]
Objective: Soybean is an excellent source of dietary peptides with beneficial effects on health. We have investigated the effect of the soybean peptides on neuroendocrine-immune network function, memory, hematology and plasma biochemistry in young healthy volunteers. Methods: This was a 6-weeks pilot study. A total of 18 healthy volunteers (9 male, 9 for female) years range (20-25 y) were recruited and received soybean peptides 8 g per day for 4 weeks and neuro-endocrine immune network function, memory , hematology, plasma biochemistry parameter were measured and analyzed at baseline(first week and second week) and 6th week. Results: When compared with the baseline, soybean peptides significantly increased the WBC counts and platelet counts. They were found to increase plasma IgA, IgG, IgM concentrations but decrease IgE and TG serum concentrations as well as the ACTH, β-EP, A, NA, DA, DOPAC level in serum. However, they increased the level 5-HA, and improved the short memory of young healthy volunteers. Conclusion: These results suggest that treatment with soybean peptides may have a positive effect on neuro-endocrine immune network function, memory, hematology, plasma biochemistry. Moreover, they do not appear to have significant toxicity.
A-121Experimental study on Vasodilation of Traditional Uygur Herb for Vitro Vascular
Lan Wei1, Jing Yuxia1, Halmurat Upur2*1 Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, 830011, China; 2Department of Traditional Uighur
Medicine, Xinjiang Medical University, Xinjiang Uighur Autonomous Region，China; *Correspondence should be addressed to [email protected]
Objective: To study on Vasodilation of Traditional Uygur Herb for Vitro vascular. Methods: Potassium chloride (KCL) with 60mmol L-1 induce vascular to shrink, and evaluation of different varieties Uygur Medicine (25μg•mL-1) to blood vessels of diastolic function. Results: The ethanol extraction from Dracocephalum moldavica, Cymbopogon nardus and Cymbopogon distans (Nees) Wats have the
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vasodilator effect on the constriction of Vascular induced by the high concentration KCl, and these three extractives relaxing rate are 52.63%, 112.66% and 57.76%, then the IC50 value are 11.79μg•mL-1, 8.23μg•mL-1, 12.35μg•mL-1. The ethanol extraction from the nymphaea alba have the vasodilator rate is 71.56%, IC50 value concentration in 16.09μg•mL-1 that have effect on the constriction of vascular induced by the NE. Conclusion: The experimental results can offer the theory basis for development and application of the Traditional Uygur Herb.
A-122Retrospective analysis of hypertension and hypertensive intracerebral hemorrhage in hospitalized patients of Uighur and Han nationnality in Hami district of Xinjiang
Aniwar 1, Abulymit 1, Wang Kun 2, Tayer 1, He Ming F2
1Hami, Xinjiang Uighur medicine hospitals, 839000, Xinjiang Uighur Autonomous Region， China; 2First People's Hospital of Xinxiang City, Henan Province, 453000, China
Objective: Using hospitalization samples to analyze the prevalence rates of hypertension and hypertensive intracerebral hemorrhage between the Uighur and Han nationality hospitalized patients in Hami of Xinjiang province. Methods: To analyze the hypertension and hypertensive intracerebral hemorrhage we use retrospective analysis method to collect the first hospitalization clinical data of the Uighur and Han nationality in Xinjiang Hami Center Hospital and the Region Uighur Hospital from January 1, 2005 to December 31, 2011. Results: Comparatively the prevalence rate of hypertension in the Uighur (4.27%)and Han (2.78%)nationality showed significant difference(P=0.00， α＜ 0.05); Compared with the prevalence rate of hypertensive intracerebral hemorrhage in the Uighur(0.99%)and Han(0.61%)nationality, they showed significant difference(P=0.00 ， α ＜ 0.05); the hypertensive intracerebral hemorrhage of the Uighur and Han patients account for 23.10 and 22.04 percent of all hypertension patients, there is no significant difference between them(P=0.50，α＜0.05). Conclusion: We conclude that in Xinjiang Hami area the prevalence rates of Uighur hypertension and hypertensive intracerebral hemorrhage is higher than that of the Han nationality. Reinforcing the prevention to Uighur patients with cardiovascular and cerebrovascular diseases, especially at higher risk has an important clinical significance to reduce the prevalence rates of hypertension and hypertensive intracerebral hemorrhage in this area.
A-123Hepatoprotective effects of Cichorium Glandulosum leaf extract against d-galactosamine-induced Liver injury in mice
Guzalnur Imam 1, Yusupjan1, Aynur1, Nurmuhammat Amat1*1Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi, Xinjiang Uighur Autonomous Region, PR China;
*Correspondence should be addressed to Nurmuhamamt Amat, [email protected]
Objective: Cichorium glandulosum,a Traditional Uighur herbal medicine, has been widely used for the treatment of chronic hepatopathy of various etiologies. Methods: The hepatoprotective activity of Cichorium glandulosum leaf extrat was evaluated againstd-galactosamine (d-GalN)-inducedhepatic
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injury inmice. GLP was administered via gavage daily for 2 weeks at doses of 100, 200 and 400mg/kg, respectively. Control groups were given the Silymarin (25 mg/kg) synchronously. Then the mice from d-GalN control and GLP-treated groups were treated with d-GalN (750 mg/kg) suspended in normal saline by intraperitoneal injection. Results: d-GalN-induced hepatic damagewas manifested by a significant increase in the activities of marker enzymes (AST, ALT) in serum and MDA level in liver (P < 0.01), and by a significant decrease in activity of SOD and GSH level in liver (P < 0.01). Pretreatment of mice with GLP reversed these altered parameters to normal values. The biochemical results were supplemented by histopathological examination of liver sections. The best hepatoprotective effects of GLP were observed after treatment with the dose of 180 mg/kg as it was evidenced from biochemical parameters and liver histopathological characters which were similar to those of normal control group. Conclusion: Results of this study revealed that GLP could afford a significant protection in the alleviation of d-GalN-induced hepatocellular injury.
A-124Influence of the Uighur Medicine sand therapy in on hemorheology of the rabbit experimental knee osteoarthritis and FEM analysis
Mahemuti D1, Halmurat Upur 2*, Kong CY1，Huang W1, Liu L1, Wei R 21 Department of Mechanical Engineering, Xin Jiang University, Urumqi,830008; 2 Traditional Uighur Medicine Department, Xinjiang Medical University, 393 Medical University Road, Urumqi 830011, Xinjiang, PR China; * Correspondence should be addressed to [email protected]
Objective: The experimental and FEM analysis were used in order to test the blood flow of osteoarthritis(OA) animal mould changed by using sand therapy. Methods: 20 New Zealand rabbit were used to build OA animal mould, then changed them into two groups randomly, group A—the control group, did not give any treatment, group B—sand therapy group, gave sand therapy for 20 days, tested the parameter of the blood flow, and built the FEM analysis mould to do the transient analysis. Results: the PS and ED P<0.01) of the blood flow increased, and RI(P<0.05) of the blood flow decreased after sand therapy; to compare group A with group B, the PS, ED(P<0.01) of the right limbs increased. The blood velocity increased when the inlet velocity increased and the shape of the blood vessel was like an egg, and at the small radius of curvature the velocity of the blood was faster than the big radius of the curvature, meanwhile, the shape of the section have different angle, it may be the reason that the blood forward spirally. Conclusion: This kind of movement contributes to flushing the vascular wall and reduces the formation of atherosclerosis.
A-125Inhibitory effect of Pomegranate Blossom Flower (PGf) extract on Influenza virus neuraminidase activity and induced Cytotoxicity
Lan Wei1，Lan Wenjun2，Halmurat Upur1,3*1Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi 830011,China; 2Institute of Biomedical
Engineering, Shandong Provincial Key Laboratory of Microbial Engineering, Shandong Institute of Light Industry, Jinan
250353,China; 3 Traditional Uighur Medicine Department, Xinjiang Medical University, 393 Medical University Road, Urumqi
830011, Xinjiang, PR China；Correspondence should be addressed to [email protected]
Objective: The objective of this study was to address the inhibitory effects of PGf water and ethanol extracts on influenza A virus neuraminidase activity and induced cytotoxicity. Methods: The inhibitory
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effect of PGf extract on influenza A virus (H3N2) neuraminidase activity was analyzed by an enzyme-promoting in vitro model. The suppressing effect of PGf extract on influenza A virus-induced MDCK cytotoxicity was assessed by crystal violet uptake method. And MNCC (maximal non-cytotoxic concentration) of the extract was assayed by MTT method. Results: Stimulation with PGf water extract can significantly inhibit the neuraminidase activity in a dose-dependent manner, at IC50=28.33±2.54 μg/mL. The cytotoxic response of MDCK cells to virus was observed to be concentration-dependently suppressed by PGf water (IC50=63.04±5.26μg/mL) and ethanol extract (IC50=67.36±6.18μg/mL). Incubated with the water extract of up to 1000 μg/mL or the ethanol extract of up to 300μg/mL, MDCK cells survival appeared to be at least 95% of the control. Conclusions: This study suggests that PGf water extract can inhibit influenza A virus induced cytotocity by antagonizing neuraminidase activity, and the mechanism of PGf ethanol extract suppressing influenza A virus needs further research.
A-126Preparation and in vitro anti-cancer activities of flavonoids from Xinjiang Glycyrrhiza Inflata
Mourboul Ablise1, Sheng Lei2, Rena Kasim1*, C.Zhidong 3, G.Xia 2, G.Miaomiao 1, X.Fangye 1, A.Abulizi 2
1Department of Medicinal Chemistry, College of Pharmacy; 2Xinjiang Key Laboratory of Molecular Biology and Endemic
Diseases, Xinjiang Medical University; Urumqi 830011, PR of China; 3 ITODYS-CNRS UMR 7086, University Paris 7, 75205
Paris, France; Correspondence should be addressed to [email protected]
The root of Glycyrrhiza inflate Bat is an important medical herb and a main constituent of Abnormal Savda Munziq (ASMq), a unique preparation of Traditional Uygur Medicine for commonly used in the treatment of various cancer patients. However, the specific contribution of Glycyrrhiza inflata on anticancer activity in ASMq prescription has not been well investigated. In this study, we investigated the in vitro anti-cancer activity of general flavonoids (GFs) and main active constituent licochalcone A (LicoA) from Glycyrrhiza inflata in human cervical carcinoma cells. GFs was prepared from Xinjiang Glycyrrhiza inflata and its finger print map specter was characterized by electro-spray ionization mass spectrometry (ESI-MS); LicoA was isolated and purified from GFs; human SiHa cervical carcinoma cells were treated with GFs and LicoA respectively; the cell viability and cellular apoptosis were determined by MTT method and flow cytometry. GCs and LicoA inhibited the growth and viability of cancer cells effectively with an upper limit of 87% for GFs and 97% for LicoA within the dose range of 25-100μg /ml detected by MTT. The cancel cell apoptotic effect was 93% for GFs and 97.4% for LicoA detected by flow cytometry. The present study demonstrated that GFs and LicoA had relatively high anti-cancer activities on human cervical carcinoma cell line. This work may provide important evidence for further screening and developing new natural anti-cervical cancer agents from the licorice species Glycyrrhiza inflate Bat; and this may provide also substantial basis for explaining anticancer effect of ASMq traditional preparation.
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(Chemical structure of licochalcone A)
References:1. Upur, H. et al. (2011). Evidence-Based Complementary and Alternative Medicine eCAM 10,1093, 1–9.
2. Yusup , A . et al. (2007). Zhongguo Zhong Yao Za Zhi 32, 1068–1071.
3. Xie, J. et al. (2010). Chemistry of Natural Compounds 46, 148-151.
4. Yi, T.Y. et al. (2009). Journal of Agricultural Food Chemistry 57, 8266–8273.
5. Ma, S.Y. et al. (2008). Northwest Pharmaceutical Journal 23, 276–278.
6. Li, H.Z. et al. (2010). Natural Product Research and Development 22, 112–114.
7. Guo, X. et al. (2011). Natural Product Research and Development 23, 49–53.
8. Sabzevari , O . et al. (2004). Chemical-Biological Interactions 148, 57–67.
9. Kolbe, L. et al. (2006). Archives for dermatological research 298, 23.
A-127Report of the eels and other drug treatment of facial paralysis embolism
Mamat Hujy1, Gao Jianping1
1Hami region Uygur hospital, Hami City, Zhongshan South Road, No. 171, 839000, China
We have been using the rice field eel to treat the facial palsy, body paralysis, limb paralysis and other diseases in our hospital from 1996. These patients daub: use the rice field eel 's blood, and at the same time, use the soup by its meat to patients to heal for facial nerve paralysis. With this treatment to improve the recovery efficiency. Next we will introduce this method and therapeutic effect. Introduction: there are four normal hilit in the Uygur Medicine, Sapra hilit(SAPRA), blood hilit (KAN), phlegm hilit (BALGAM) and Sawda hilit (SAWDA). The four hilit are distributed, and constant drain in the body, and then continuously produce again, they keep a certain proportion and play an important role for healthy. Once the balance were disordered, people will get sick. So in Uygur Medicine treatment, adjust the temperament and hilit as the main treatment method.
A-128Screening of quantitative diagnosis indices of Typing Abnormal Hilit syndrome in Uygur Medicine using delphi method to survey: 180 cases of malignant tumor
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Ayinuer Mamatisidike1, Halmurat Upur1* ，Erxiding2, N.Aji 3, R.Abuwayiti 4, M.Ali 5, W.Abudulaihaiti 1,
You Lina 1, Y.Na 1
1 Traditional Uighur Medicine Department, Xinjiang Medical University, 393 Medical University Road, Urumqi 830011, Xinjiang,
PR China; 2First Affiliated Hospital of Xinjiang Medical University Oncology department Urumqi 830011, China; 3Hospital of
traditional Uighur medicine, Xinjiang Yarkan 844700; 4Hospital of traditional Uighur medicine, Xinjiang Karkax 848000; 5School
of public health, Xinjiang Medical University, Xinjiang Hutan 848000, China; Correspondence should be addressed to
To set up abnormal Hilit typing quantization diagnostic criteria of Uygur Medicine to the malignant tumor patients, accordance with free disease clinical diagnostic information, 180 malignant tumor patients were selected to the study of quantifying diagnosis specialist counseling. The selected factors were analyzed by modified multi-categorization logistic regression analysis determined symptom-complex factors dedication degree to the symptom-complex factors main and subsequent symptoms of abnormal Hilit of malignant tumor were discern by OR value. As a result, the meaningful symptoms were found in all the abnormal Hilit syndromes which is done according to the OR value. For abnormal savda type Malignant Tumor: Chief symptoms are less eyes expression, dim lips, cyanotic tongue quality, dark and gloomy fur, greenish urine. Subsequent symptoms are not peaceful in mind, cooler skin, thin tongue, dry stool, sweating at night, dreaminess, nightmare. For abnormal kan type Malignant Tumor: Chief symptoms are reddish complexion, lack of eyes expression, dark red tongue quality, hotter skin, moist and soft skin. Subsequent symptoms are thick､strong pulse, sleep less. For abnormal balgham type Malignant Tumor: Chief symptoms are low spirit and unpleasant, pale complexion, sticky mouth, cooler and moist skin quality, thick and low colored urine in large amounts, much need for sleep. Subsequent symptoms are pale and tasteless mouth, frequent and more urine .For abnormal sapra type Malignant Tumor: Chief symptoms are easy to anger, yellowish conjunctiva, pale lips, dry mouth, dry and hotter skin quality, thready short fast pulse. Subsequent symptoms are bittersweet taste, thin tongue, dry and yellowish stool. As a result the feature of excess syndromes of the four abnormal Hilit are consistent with clinical practice and the symptom quantitative diagnosis of the abnormal Hilit excess syndromes provides the objective evidence for clinical practice.
A-129The curative effects of 51 cases with Uighur Medicine therapy on Urticaria
Mahmutjian Ahmat1, Mamatjian Zayit1
1Aksu Prefecture Uighur Medicine Hospital, 843000, Xinjiang China
Urticaria commonly referred to as hives, which is a kind of skin rash notable for pale red, raised, itchy bumps. Hives are frequently caused by allergic reactions; however, there are many non-allergic causes. Most cases of hives lasting less than six weeks (acute urticaria) are the result of an allergic trigger. Chronic urticaria (hives lasting longer than six weeks) is rarely due to an allergy. The majority of patients with chronic hives have an unknown (idiopathic) cause. Perhaps as many as 30–40% of patients with chronic idiopathic urticaria will, in fact, have an autoimmune cause. Acute viral infection is an another common cause of acute urticaria (viral exanthem).We observed the effect of Uighur medical bathe on
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the urinary treatment. According to the cause, 51 cases of patients were treat with mature urticaria agent, clearing agent after the treatment with major drug merger besmear medicine, Uighur medical bathe treatment are effective to urticaria treatment, with the proportion reached 90%.
A-130The systematic reviews of the Uighur Medical abnormal humoral typing to the treating knee osteoarthritis
J.Xiaoyue 1, M.kelimu 1, G.Shimin 2, W.Ying 3 1The Sixth Affiliated Hospital of Xinjiang Medical University 830002,China; 2Yunnan Institute of Traditional Chinese Medicine
650223,China; 3College of Pharmacy in Xinjiang Medical University 830019, China
Objective: To evaluate systematically the efficacy and safety of treatment osteoarthritis knee based on abnormal humoral typing of Uighur Medicine. Methods: We searched the Chinese Biomedical Database (CBM) 、 Database(VIP) 、 Chinese HowNet database(CNKI) and Wanfang Database (WanfangData) to build a database in December 2011, which were included in the published literature on abnormal humoral theory of Uighur Medicine clinical treatment of knee osteoarthritis. Literature screening and data extraction were performed by two researchers independently, and then cross-checking. If there were differences on reaching agreement the third-party’s opinions were recommended for discussion or reference. By qualitative research methods the information and results were summarized. Results: 688 records were retrieved. The analysis results show that the Uighur medical abnormal humoral typing theory to treat knee osteoarthritis in Xinjiang has been used for a long time and the effective rate is 84%. Conclusion: The clinical treatment of Uighur medical abnormal humoral theory to knee osteoarthritis has been carried out by some researches, but its quality is not very high. The demonstrated efficiency will be improved.
A-131Treatment of 225 cases of hair loss accordong to Uighur Medical temperament theory analysis alopocia
Sidik Ramat, Nuermanguli Aly1Yecheng Uighur Medicine Hospital, 844900, Kashgar, Xinjiang, China
Objective: Observe a clinical observation to treat hair loss (alopecia areata) disease by using the Uighur Medical temperament analysis theory. Methods: Use the Uighur Medicine which has the function of increasing the hair internally and externally. Results: Among 255 cases , 136 cases were recovery(53.34%), and 80 cases were markedly effective (31.37%), 29 cases were effective (11.37%), 10 cases were invalid(3.92%), the total effective rate were 96.08%. Conclusion: To treat hair loss (alopecia areata) disease, combined analysis of the patient's abnormal temperament, innate qualities, gender, age, history and other factors is needed. Combined with adjustment of the mediation abnormal temperament, the internal and external medicine therapy may enhance the curative effect.
A-132
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Tradition, current situation and perspective of Traditional Uighur Medicine in China
Nurmuhammat Amat1, Palidan Wushuer 2, Aziz R A1, Halmurat Upur1*1Traditional Uighur Medicine Institutet of Xinjiang Medical University, 393 Medical University Road, 830011, PR China;2
Neurology Department of Xinjiang Medical University affiliation Traditional Chinese Medicine Hospital, 116 Huanghe Road,
830000, PR China; Correspondence should be addressed to [email protected]
Complementary and alternative medicine (CAM) is becoming more and more important in improving human health. CAM which includes Traditional Uighur Medicine (TUM) is very popular among the population in China and the Central Asian countries. With its history of thousands of years, TUM has contributed to the health of the people in the West of china and Central Asia. Historical and current surveys indicate that Xinjiang Uighur Autonomous Region of China has been distinguished throughout the generations with a rich inventory of natural medicinal herbs. TUM, including historical background, Current Situation and Perspective of Traditional Uighur Medicine in China, covers the medical innovations introduced by Uighur physicians in the field of theory, herbal medicine therapies and development of Traditional Uighur Herbal Medicine in Xinjiang Uighur Autonomous Region.
References:
1. Upur H, Yusup A (2003) Theory of Hilit and Mizaj on Uighur Modern Research on it. Science and Technology Press. Urumqi,
China.
2. Upur H, Denise D, Nurmuhammat A (2010) Uighur Medicine. ITA Rhythm.St. Petersburg, Russia.
A-133Treating report about 190 patients with kidney stone patients treated by Uighur Medicine
Abduwayit Nurahmet1
1 Kashgar Prefecture Yecheng Uighur Medicine Hospital, The Yecheng South Jiefang Road NO.16, 844 900, china
Objective: To observe the effect of Uighur Medicine named Supopi Mupetti Hasat at treating patients with kidney stones. Methods: From January 2005 to August 2010 we have treated 190 patients with kidney stones, during which we identified and analyzed the relationship between the cause of kidney stones, ages, gender and temperament of patients. And then treated them by Uighur medicine named Supopi Mupetti Hasat and observed the effect of removing kidney stones. Results: After using the Uighur Medicine named Supopi Mupetti Hasat on 190 patients with kidney stone we found that 117 of them (61.6%) cured, 64 of them (33.7%) improved and 9 of them had (4.7%) no effects. It means that the treating effect of the medicine reached 95.3%. Conclusion: Kidney stones are clinical common diseases, and this abnormal renal function which has close relation to obstacles of Material exchange caused by disordered Fluid balance. Kidney stone has no selection object, young and old, rich and poor, all of them are equal to get this disease. If not treated in time, it will cause obstruction of Urinary track and infection and it will lead to no urine, uremia, nephritis, hydronephrosis. At last it will get worse and worse and causes Renal exhaust. Modern medicine use surgical treatment as the main treatment. Our Uighur Medicine named Supopi Mupetti Hasat made by pure natural medicinal herbs has better effect of breaking kidney stone and remove it, besides diuresis, analgesia, diminish inflammation, regulate humor. The effect of the Uighur Medicine is better and faster than similar medicines, and has
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less adverse reactions.
A-134A clinical report of 31 cases using Uighur Medical treatment of herpes zoster
Nuermangul Alya1, Sidik Ramat1, Tajigul Mamat1
1Kashgar Yecheng Uighur Medicine Hospital Dermatology, South Jiefang Road NO.16 Yecheng , 844900,China
Objective: To observe the Uighur Medical treatment for herpes zoster clinical effect. Methods: with anti-inflammatory, analgesic, promote skin healing inside and outside in Uighur Medicine. Results: Of the 31cases, 9 cases were cured (29%),15 cases were valid (48.4%), 6 cases were effective (19.4%) ， and invalid in 1 case (3.2%). Conclusion: the therapy of herpes zoster disease, comprehensive analysis in patients with disorders of fluid balance disorders, temperament, gender, age, medical history and other factors, adjust the body fluid balance disorders and temperament disorders, with the combination of internal and external medicine therapy, may improve the curative effect.
A-135Uighur Medicine fusion of East-West cultural factors
Wang Xingyi11Shanghai University of Traditional Chinese Medicine Institute of Chinese literature, Cai Lun Lu 1200, Pudong New Area,
Shanghai, 201203, China
Through analyzing the unearthed medical literatures from Sinkiang, catalogs of historical chronicles, codex handed for generations and medical preservations from contemporary Uighur Medicine, which were discovered to contain the Oriental cultures such as cultures from Central China, India, Persia, Arab and Occidental cultures such as Hellenism, the article proved that the Traditional Uighur Medicine integrates the cultural factors of Orient and Occident.
A-136The effect of Abnormal Savda Munziq on Hippocampus BDNF, 5-HT1A mRNA expression in depression animal models and Abnormal Savda Syndrome animal models
Hanzohra Upur1, Nurmuhammat Amat 1, T.Kamil 1, A.Rozi 1, Abdiryim Yusup 1*, Halmurat Upur 1* 1Traditional Uighur Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;*Corresponding
authors: Abduryim Yusup，Halmurat Upur, Traditional Uighur Medicine Department, Xinjiang Medical University, 830011
Urumqi, E-mail: [email protected]，[email protected]
This paper studies the effect of Abnormal Savda Munziq on mRNA expression of Hippocampus BDNF, 5-HT1A in depression animal models and Abnormal Savda Syndrome animal models. The mRNA expression of Hippocampus BDNF was detected with Real-time quantitative Polymerase Chain Reaction and mRNA expression of Hippocampus 5-HT1A was detected with RT-PCR. Compared to the control group, mRNA expression of Hippocampus BDNF, 5-HT1A was down-regulated in depression animal models and Abnormal Savda Syndrome animal models. The mRNA expression of Hippocampus BDNF, 5-HT1A was up-regulated in the Abnormal Savda Munziq medium-dose, high-dose groups of
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depression animal models and Abnormal Savda Syndrome animal models. There was a significant difference on the mRNA expression of Hippocampus BDNF in depression animal models and Abnormal Savda Syndrome animal models (P<0.01). There is not a significant difference on the mRNA expression of Hippocampus 5-HT1A in depression animal models and Abnormal Savda Syndrome animal models (P>0.05). The Abnormal Savda Munziq is probably related to the up-regulation of mRNA expression of Hippocampus BDNF, 5-HT1A in depression animal models and Abnormal Savda Syndrome animal models. It was revealed that the intrinsic link of Abnormal Savda Syndrome and depression was at the molecular level.
A-137The construction of the Uighur pharmaceutical scientific research information support platform system based on Cloud Technology
Zhou Yi1,2, Dilxat Yimit1, Luo Xueqiong2, Chen Guozhong 1, Xue Jie1, Du Shouhong 1, Sen Gan 1, Nurmuhammat Amat2，Abdiryim Yusup2, Halmurat Upur 2*1 Xinjiang Medical University; Urumqi 830011, China; 2 Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou
510080, China; 2 Traditional Uighur Medicine Department, Xinjiang Medical University, 393 Medical University Road, Urumqi
830011, Xinjiang Uighur Autonomous Region，PR ChinaCorrespondence should be addressed to [email protected]
In order to promote the inheritance and research of traditional medicine in Xinjiang fastly, this project will construct the Uighur pharmaceutical scientific research information support platform system based on cloud technology for Uighur pharmaceutical scientific in Xinjiang. The system's information source is making up with the private cloud which made up with our school/our school's secondary college/hospital site and the public cloud which made up with Domestic/foreign public website/medical professional website. So it can collect, analysis, integration and storage the latest medical information automatically. According to the needing of the Uighur Medicine disciplines scientific research, the cloud platform provides the researchers with the latest information which they concerns, such as Basic Medicine, Complexity of disease pathogenesis, Standardization of prevention in Traditional Chinese Medicine/ Uighur Medicine/Western Medicine, New medical technology, Epidemiological studies, Medical Conference, Medical hotspots, Announcements of Project Application and so on. So that the Uighur Medicine disciplines scientific researchers can master the latest medical research dynamic and track the progress in their research work rapidly, comprehensively, and accurately. At the same time, the construction of the virtual community and exchange platform can further promote the advancement of the researchers' work and express the modern information technology to provide comprehensive, efficient and accurate information support role in standardization of prevention of Traditional Chinese Medicine, Uighur Medicine and Western Medicine.
A-138Chemical Constituents in Essential Oil from Hyssopus officinalis.L by Steam Distillation and SFE-CO2 by GC-MS
Mahsum Ekim1，Nurjan1, Abdiryim Yusup*1Traditional Uighur Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
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To compare chemical constituents of the essential oil of Hyssopus officinalis.L to be extracted by steam distillation (SD) and supercritical-CO2 fluid extraction (SFE-CO2). The chemical components of the essential oil were separated and identified by GC-MS, Their relative contents were determined by normalization of peak area. In essential oil extracted by SD 65 compounds were detected, with the higher levels of linoleic acid(13.83%)，palmitic acid(13.80%),(+)- pulegone(8.31%)， (+)the Spartak alcohol(6.27%)，20 alkyl(5.34%)，lemongrass acid(4.07%). In essential oil extracted by SFE-CO2 34 compounds were detected, with the higher levels of the γ-Valley sterol(31.882%) ， 21 alkyl(19.953%)， linolenic acid(16.279%),hexadecane(15.939%)， palmitic acid(13.80 %)， linolenic acid ethyl ester(8.766%). The constituents of essential oils extracted by SFE- CO2 were different from that of extraction by SD. The result provided evidence for understanding the constituents of essential oils and explitation of Hyssopus officinalis.L .
A-139Preliminary study on the anti-inflammatory effect of the extracts of Hyssopus officinalis.L
Mahsum Ekim1，Nurjan1, Abdiryim Yusup1*1Traditional Uighur Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
Objective: To study the anti-inflammatory effect of extracts from Hyssopus officinalis.L to screen the fraction anti-inflammatory activity. Methods: Through mouse anti-inflammatory, cough, expectorant experiment to observe the anti-inflammatory, cough, expectorant effects of Hyssopus officinalis.L. water, ethanol extract and the ethyl acetate extract parts. Results: Compared with model group, Hyssopus officinalis.L. water, ethanol extract and the ethyl acetate extract had anti-inflammatory, cough, expectorant effect (P <0.05) and the role of the ethyl acetate extract was more significant. Conclusion: The ethyl acetate extract from Hyssopus officinalis.L have favourable anti-inflammatory effect.
A-140Abnormal Savda Munziq Improves the Biological Syndromes of CUMs induced Depression Animal Models
Abdiryim Yusup1, Abduwayit Nurahmet1, Guzalnur Imam1, Halmurat Upur1*1Collage of Traditional Uighur Medicine, Xinjiang Medical University, 830011, Urumqi, Xinjiang Uighur Autonomous
Region，China. Correspondence should be addressed to [email protected]
Objective: To observe the effect of Abnormal Savda Munziq (ASMq) on biological syndromes of chronic unpredictable mild stress (CUMs) induced depression animal models. Methods: The 84 SD rats were randomly divided into 6 groups with 14 in each group: normal control group, depression model group, groups of Abnormal Savda Munziq granular for high, medium and low dosages (6, 3 and 1.5 g/kg.BW, respectively) and the positive control group (Fluoxetine, 3.5mg.kg-1). Except for the normal control group, the rats in all other groups were given chronic unpredictable mild stress (CUMs) to build the depression animal models. The positive control group and the Abnormal Savda Munziq groups were
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orally administrated for 24 days, once a day. To observe the biological syndromes such as general states, and to measure the body weight, food intake and water intake of animals in each group. Results: Compared with the control group, the model group animals appeared the biological syndromes such as loss of body weight, hair disorder and dull, cooler skin, purple tongue constipated, slow weight gaining, reduction of food intake and water intake. The biological syndromes such as slow weight gaining, reduction of food intake and water intake of the rats in Abnormal Savda Munziq high dosage group and positive control group were improved than that of model group. Conclusion: The biological syndromes of depression animal models were improved effectively by the administration of Abnormal Savda Munziq granular.
A-141Abnormal Savda Munziq, an Herbal Preparation of Traditional Uighur Medicine, Regulates the Neuroendocrine Function of CUMs induced Depression animal models
Abdiryim Yusup1, Hanzohra Upur1, Guzalnur Imam1, Halmurat Upur1*1Collage of Traditional Uighur Medicine, Xinjiang Medical University, 830011, Urumqi, Xinjiang Uighur Autonomous Region
China .Correspondence should be addressed to [email protected]
Objective: To observe the effect of Abnormal Savda Munziq (ASMq) on neuroendocrine function of chronic unpredictable mild stress (CUMs) induced depression animal models. Methods: The 84 SD rats were randomly divided into 6 groups: Group 1 served as normal control, administered with normal saline. Group 2 served as negative control, administered with normal saline. Group 3 served as positive control, administered with Fluoxetine at 3.5mg/kg.BW. Groups 4, 5 and 6 served as ASMq high-dose, ASMq medium-dose and ASMq low-dose group, administered with ASMq at 6, 3 and 1.5 g/kg.BW, respectively. All the groups were orally administered for 24 days, once a day. Except for the normal control group, the rats in all other groups were given CUMs to build the depression animal models. Rats were sacrificed at 24st day and the neuroendocrine function was analyzed for serum levels of CORT, ACTH, AD, ß-EP, NE, BDNF, DA and 5-HT. Results: Results showed that the serum level of AD, ß-EP, NE, BDNF, DA and 5-HT in negative control group were significantly decreased (P<0.05), CORT and ACTH were significantly increased (P<0.05). The serum level of AD, ß-EP, NE, BDNF, DA and 5-HT were increased, and the serum level of CORT and ACTH were decreased in ASMq high-dose group (P<0.05, respectively). Conclusion: It suggested that ASMq had antidepressant effect on CUMs induced depression animal model by regulating neuroendocrine function of depression animal.
A-142Clinical Research on Treatment of Knee Osteoarthritis by the instrument of Heat Conduction Sand instrument in Uighur Medicine
He Chunhui1, Halmurat Upur1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;* Correspondence should be addressed to [email protected]
Objective: To observe the curative effect and Knee function improved integral evaluation and the influence of the hemorheology by the instrument of heat conduction sand treatment in Uighur Medicine
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on knee osteoarthritis and reducing the dosage of using western medicine, for the sake of exploring its mechanism. Methods: The 120 cases of KOA patients were randomly divided into treatment group and control group, 60 cases in each group: the sand treatment group: patients were treated for 30 minutes once a day. The period of treatment was 10 days. The control group: patients received TDP radiation therapy, its treatment method and time was the same as treatment group. Both groups took oral celebrex capsule 200 mg once a day. Observe function evaluation scores, clinical efficacy and the influence of the hemorheology of two groups before and after treatment. Results: compared the total curative effect of two groups, the total effectiveness rate of treatment group is 91.9%; and the control group was 83.3%, the difference was statistically significant through two groups’ comparison (P< 0.05), which indicates that both groups can improve the knee function, but the treatment group was better than control group; Compared the improvement of two groups’ learn index after treatment, the difference was statistically significant (P < 0.05); which explained the treatment group in improving hemorrheology index situation was better than control group. Conclusion: 1. the instrument of heat conduction sand treatment in Uighur Medicine is better in stopping pain, fluctuation building ability, swelling and buckling Angle and stiffness in a degree of contracture of knee osteoarthritis treatment, its curative effect is better than the TDP radiation therapy. 2. KOA patients often associated with abnormal blood rheology, blood is at high condensation state, Uighur conduction heat effect sand therapy apparatus can improve the whole blood viscosity index of patients with osteoarthritis of the knee joint, through the sand cure heating and Traditional Uighur drug absorbed through skin, can improve local blood circulation, acetanilide detumescence.
A-143Effect of pomegranate ethyl acetate extract on the function of rat islet cells
Yao Hai1, Yang Weixing3, Xu Yonghua4, Wang Ye1, Ren Jun5, Mao Xinmin1, Yi Jingyang1, Han Xue1, Ma Xiaoli1, Wang Lifeng 1, Wang Changhui2, Li Linlin1*1Xinjiang Medical University, Shihezi Renmin Hospital, Xinjiang Uighur Autonomous Region, China; 2First Affiliated Hospital of
Xinjiang Medical University, 393 Xinyi Road, Urumqi, Xinjiang Uighur Autonomous Region, China; 3Changji Health School in
Xinjiang; 4Urumqi Military Hospital of Lanzhou District, 359 Youhao North Road, Urumqi, Xinjiang Uighur Autonomous Region,
China; 5Wyoming University, USA;* Correspondence should be addressed to [email protected]
Objective: The present study investigated the effects of pomegranate ethyl acetate extract (PEE) on the function and cell viability of primary rat islet cells. Methods: PEE was extracted using 95% ethanol and a series of organic solvents. Primary isolated rat islet cells were cultured in high glucose or high fat conditions with or without PEE. Cell viability was determined by MTT assay. Insulin secretion was assessed using radioimmunoassay. Results: Insulin levels were significantly higher in cells treated with high glucose + PEE, palmitic acid + PEE, and olein acid + PEE compared to the cells treated with high glucose, palmitic acid, or oleic acid alone, in which groups the insulin levels were markedly less than that of the normal control group (P < 0.01). Conclusions: PEE promotes islet cell growth and insulin secretion. These results suggest that PEE can protect islet cell function in both high glucose and high fatty acid environments.
A-144Establishment of hepatocarcinoma carrying abnormal savda animal model and it`s
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morphological study
Yusup Tuerxun1, Halmurat Upur1*1Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;* Correspondence should be addressed to [email protected]
Objective: To establish combining hepatocarcinoma disease and abnormal savda syndromes rats model, and investigate the effect of abnormal savda on inducing and developing process of hepatocirrhosis phase in this model. Methods: Under the direction of Traditional Uygur Medicine, multi complcated induction methods were used to establish abnormal savda syndromes rats model for three weeks, using DEN further to establish combining hepatocarcinoma and abnormal savda disease rats model till hepatocirrhosis occurs, the animals were anesthetized at 5, 7, 9, 11 weeks under the eyes of venous blood, the appearance of rat liver In HE staining under light microscope and transmission electron microscopy, liver morphology and cell ultrastructure were observed dynamically. Results: Both in the model control group and combining disease and abnormal savda syndromes model group there are the pathological changing process of hepatoxic lesion､hepatic proliferation､hepatocirrhosis, but the pathological changes of the liver surface in the combining disease and abnormal savda syndromes model group is faster than model control group. At the same time, the dropsical level of liver cell , typeless hyperplasia, fake foliole level in liver, the ultrastructural changes of decreasing hepatin and chondriosome in liver cells in combining disease and abnormal savda syndromes model group are than those in the model control group. Conclusion: These findings indicate that in the process in which establishing abnormal savda disease rat model serving as a base, using DEN furthur to establish combining hepatocarcinoma disease and abnormal savda syndromes rats model, the abnormal savda hilit accelerates the inducing and developing process of hepatocyte to hepatic Cellular proliferation to hepatocellular carcinoma and this process is similar to the process of humans hepatocarcinoma. It is also stable and reliable.
A-145Metabonomic Studies on Abnormal Savda Patients with Type 2 Diabetes Mellitus Using NMR Spectroscopy
Mao Xinmin1, Lang Jun2, Ma Xiaoli3, Li Linlin2, Han Xue2, Halmurat Upur1*, Eli Kurban4, Tursun Turgun4, Mamtimin Batur3,Yunuxi Kuresi2, Wang Lifeng2, Jiao Yi2, Wang Ye2, Yi Jinyang2
1 College of Chinese Traditional Medicine, Xinjiang Medical University, Urumqi ， Xinjiang Uighur Autonomous Region ， 830011, China; 2Basic Medical College, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
RegionChina; 3 College of Pharmacy, Xinjiang Medical University, Urumqi 830011 ， Xinjiang Uighur Autonomous Region, China; 4College of Uighur Medicine, Xinjiang Medical University, Urumqi 830011, China; *Correspondence should be
addressed to [email protected]
To investigate the metabolites changes in urine of patients carrying type 2 diabetes mellitus with abnormal savda based on the theory of Traditional Uighur Medicine. 1H Nuclear Magnetic Resonance (NMR) based on metabonomic analysis was performed on urine samples obtained from the abnormal savda patients with type 2 Diabetes Mellitus and savda volunteers with normal plasma glucose. The Orthogonal Partial Least-Squares discriminant analysis (OPLS-DA) was fulfilled to distinguish the
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metabolic phenotypes. The results show that the metabolites in urine of abnormal savda patients with type 2 diabetes mellitus are significantly changed. The concentrations of glucose, taurine, betaine are increased (P<0.05); Meanwhile, the concentrations of some amino acids, creatinine, hippurate, citric acid and dim ethylamine are decreased (P<0.05) as compared with savda volunteers with normal plasma glucose. The findings indicate that there are great differences in the urine metabolites between the abnormal savda patients type 2 diabetes mellitus and savda volunteers with normal plasma glucose by using NMR; and reveal that endogenous compounds may be the ingredients of abnormal savda patients with type 2 diabetes mellit
A-146Studies of chemical constituents and antioxidant activity of Jujubae Fructus in Xinjiang
Wang Dongdong1, Tian Shuge1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;* Correspondence should be addressed to [email protected]
Objective: To study chemical composition and antioxidant activity of six cultivars of jujubae fructus from different areas in Xinjiang Uighur Autonomous Region, provide a more stable and reliable method for quality control, and provide a reference for clinical medicine and food processing. Methods: Total flavonoids and Polyphenolic contents were measured using spectrophotometry; rutin, oleanolic acid, ursolic acid contents were measured using high performance liquid chromatography (HPLC method), cyclic adenosine monophosphate (cAMP) contents were measured using Rapid Resolution Liquid Chromatography (RRLC); to determine metal elements by Microwave Digestion-Flame Atomic Absorption Spectrometry(FAAS); to optimize the extract technology of total flavonoids from jujubae fructus; to study Separation and purification process of total flavonoids from jujubae fructus using macroporous adsorption resin; to utilise three types of antioxidant activity models (clear DPPH free radical, hydroxide radical and SOD active effect) to do antioxidant activity screening to water extracts of jujubae fructus. Results: There were different levels of total flavonoids and polyphenolic contents in six cultivars of jujubae fructus; the contents of rutin, oleanolic acid, ursolic acid and cyclic adenosine monophosphate in six cultivars of jujubae fructus in Xinjiang existed obvious difference; the metal element contents in six cultivars of jujubae fructus were determined; through 2 times of refluxing with 70% alcohol, of which the amount was 12 times of sample, and 1h for each refluxing , the best extraction efficiency was obtained. The experimental results of the static and dynamic adsorption-desorption experiments show that D101 resin possesses higher absorption and desorption capacity. The results of the present work indicate that the water extracts from six cultivars of jujubae fructus had different levels of antioxidant activity. Conclusion: The study determined contents of total polyphenolic, total flavonoids, rutin, oleanolic acid, ursolic acid, cyclic adenosine monophosphate and metal elements in six cultivars of jujubae fructus, optimized the extract technology of total flavonoids and studied separation and purification process of total flavonoids from jujubae fructus using macroporous adsorption resin, studied antioxidative actions of water extracts from six cultivars of jujubae fructus in vitro, and offered theory evidences for quality control and further study of jujubae fructus.
A-147Studies on Chemical Constituents and anti-tumor of essential oil from the seeds of Ferula
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sinkiangensis
Wang Yue1, Jiang Lin1, Li Xiaojin1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: For the further study of the seeds of F.sinkiangensis pharmacological effect and to lay the foundation for its exploitation, the chemical composition and naphtha antitumor activity of xinjiang ferulic seeds were researched in this paper. Methods:1)The extracted mixture of the seeds of F.sinkiangensis from purified water,95％ethanol and petroleum ether were studied by test tube and filter paper method. The chemical constituents of the seeds of F.sinkiangensisK is primarily demonstrated by precipitation reaction or color reaction with various indicator.2) The constituents were isolated and purified by column chromatographies and their structures were identified by physicochemical properties as well as spectral data.3); The essential oils were extracted by the method of hydrodistillation and analyzed by gas chromatography-mass spectrumetry (GC-MS). 4) The conventional MTT method was used to analyse essential oils inhibitory action face CACO2 proliferation in vitro and count IC50. Results: 1) The preliminary test indicates that the seeds of F.sinkiangensisK may contain chemical constituents such as volatile oil, triterpenes, sterides, coumarin, terpene lactone, flavonoids, anthraquinones, phenols, amino acids,sugar etc. 2) Twelve compounds were isolated from the seeds of F. sinkiangensis and their structures were elucidated as stigmasterol(Ⅰ), siknianone(Ⅱ), stigmasterol-3-O-glucoside(Ⅲ), colladonin(Ⅳ), ferulic acid(Ⅴ),7-hydroxy oumarin(Ⅵ). 3) 26 compounds were identified in F.sinkiangensis, comprised 99.001% of total essential oil; 21 compounds were identified in F.fukangensis, comprised 100% of total essential oil; 25 compounds were identified in Ferula ovina, comprised 99.459% of total essential oil. n-propyl sec-butyl disulfide is the main component in three cultivars seeds of Resina Ferulae,accounting for 55.875%、49.797%、53.781%, respectively. 4) the essential oil from the seed of three Xinjiang Ferula asafetida to the CACO2 have a dose dependent effect in vitro, the essential oil IC50 of F. snkiangensis、F. fukangensis、F. ovina were16.82±1.17 ug/ml、12.28±1.41μg/ml、10.75±1.02μg/ml, respectively. Conclusion: 1); This experiment provide foundation for further study of its bioactivities to preliminarily determine the chemical components of the seeds of F. sinkiangensis. 2) Compounds Ⅰ,Ⅲ,Ⅳ,Ⅴ,Ⅵwere isolated from the seeds of F. sinkiangensis for the first time. 3) Compared the essential oil chemical constituents with the seed Three Xinjiang Ferula asafoetida, we can see the bases of three asafetida are the same , with little diversity among three cultivars seeds of Resina Ferulae from Xinjiang. 4) The essential oil of this three kinds of Xinjiang F. asafetida seeds have strong anti-tumor activity in vitro, for the size of the tumor suppressor role in turn F. ovina> F. fukangensisK> F. snkiangensis K.
A-148Study on Quality Standard and Fingerprint of Ziziphora clinopodioides Lam
Yu Qian1, Tian Shuge1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: This study was designed to examine the chemical composition and antioxidant activity of
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Uighur Medicine Ziziphora clinopodioides Lam., to further expand the pharmacology effect of Ziziphora clinopodioides Lam. and to establish firm fundament of reasonable developing and utilizing to Ziziphora clinopodioides Lam. in Xingjiang. Methods: According to the 2010 version of Pharmacopoeia, there are identifications of the original plant of ziziphora clinopodioides lam and descriptions of their properties; It's stems, leaves and powder for microscopic identification, it's water extract and alcohol extract of the physical and chemical identification and in contrast to linarin, caffeic acid and rosmarinic acid on TLC. The 7 batches of ziziphora clinopodioides lam of moisture and ash were determined. GC / MS techniques are used to analyze four different origins Ziziphora clinopodioides Lam essential oil chemical composition, and the method of liquid chromatogram to determine contents of pulegone, caffeic acid, rosmarinic acid, diosmin, Linarin in Ziziphora clinopodioides Lam. from ten different origins of production have been applied in Xinjiang to study on fingerprint of Ziziphora clinopodioides Lam. Results: Check in formulating moisture was less than 9%. Total ashes were less than 12%. Acid-insoluble ashes were less than 4%. 9-20 compounds identified from the essential oil, the contents of pulegone, caffeic acid, rosmarinic acid, diosmin, Linarin in Ziziphora clinopodioides Lam. from ten different origins of production in Xinjiang are obviously different. The determination of the acquisition of ten different origins of Ziziphora clinopodioides Lam, calibration of the 11 common peaks, and the establishment of the Ziziphora clinopodioides Lam fingerprint chromatogram were made. Conclusion: This project conducted a preliminary study for Xinjiang origin Ziziphora clinopodioides Lam quality standards. The study determined contents of pulegone, caffeic acid, rosmarinic acid, diosmin, Linarin in Ziziphora clinopodioides Lam from ten different places of production in Xinjiang, and laid the foundation for the lip herbs to establish the standards of scientific quality. Meanwhile, the fingerprinting studies were firstly conducted to Ziziphora clinopodioides Lam. It offered scientific evidences for establishing scientific quality standard of Ziziphora clinopodioides Lam.
A-149Study on the Uighur Medicine Compound of the Anticancer Activity Parts of Abnormal Black Munziq—Based on the “TCM Components” Mode
Yang Juan1, Zhang Fan1, Halmurat Upur1* 1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;* Correspondence should be addressed to [email protected]
Objective: The purpose of this paper is to make the preparation of the anticancer activity parts of Abnormal Black Munziq study on its chemical components and determine the main components’ content to evaluate its material composition, prepare its standard components and proceed chemical characterization, analyse the quantitative and qualitative, and create Information database. Method: 1. Gradient elution on Abnormal Black Munziq to obtain its anticancer active parts using polyamide column chromatography and solid phase extraction technology. We used polyamide column chromatography to prepare the extract of Abnormal Black Munziq, used solid phase extraction, and separated the extract to prepare the anticancer activity parts. 2. We prepared the standard components of anticancer activity parts by using semi preparative high performance liquid chromatography. 3. We proceed chemical characterization with standard components and used UV and HPLC. 4. We analyzed the qualitative of anticancer activity parts of Abnormal Black Munziq and used TLC. 5. We analyzed the quantitative of the anticancer activity parts and used HPLC. 6. We drew up Prescription database software, input all
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kinds of information of Abnormal Black Munziq and established Data-base using C# database programming language. Results: 1. the anticancer activity parts of Abnormal Black Munziq were acquired. 2. 18 standard components of the anticancer activity parts of Abnormal Black Munziq were prepared. 3. The standard components to proceed chemical characterization of UV and HPLC were directed. 4. The qualitative analysis result of TLC indicated that the anticancer activity parts of Abnormal Black Munziq contained gallic acid、caffeic acid、rutin、 licorice glycoside、quercetin- 3- O- glucoside、 isoferulic acid、quercitrin、hesperidin、 rosmarinic acid、glycyrrhizic acid. 5. The result of quantitative analysis indicated that every gram of the Anticancer activity parts of Abnormal Black Munziq contained gallic acid 4.85mg、caffeic acid 6.99mg、rutin 27.23mg、 licorice glycoside 20.24mg 、 quercetin- 3- O- glucoside 12.25mg 、 isoferulic acid 16.08mg 、 quercitrin 7.75mg、 hesperidin 58.16mg 、 rosmarinic acid 57.96mg 、 glycyrrhizic acid 19.29mg. 6. The information database of Abnormal Black Munziq was established. Conclusion: 1. Abnormal Black Munziq with gradient elution using polyamide column chromatography and SPE is a simple and effective way to acquire its anticancer activity parts. 2. To complex things, the most simple and direct way to understand them is decomposition and simplification. Semi preparative high performance liquid chromatography, which has the feature of high-efficiency, speediness and automatic operation has an important position in the area of separation and purification. 3. Systematic chemical characterization of Traditional Chinese Medicine composition is a way of using the modern separation technology and method to proceed scientific and effective characterization research. UV and HPLC, which play an important role in the area of complex samples analysis are also the most powerful tools to solve the problem of the area. 4. The anticancer activity parts have high content of flavonoids substances. Qualitative analysis by TLC have advantages of shortcut, trace, high sensitivity and good separation efficiency and so on. 5. On the anticancer activity parts of Abnormal Black Munziq we undertook quantitative analysis of gallic acid、 caffeic acid、 rutin、 licorice glycoside、 quercetin- 3- O- glucoside、 isoferulic acid、 quercitrin、 hesperidin、 rosmarinic acid、 glycyrrhizic acid and used HPLC. The methods are accurate, precise and are all consistent with the requirement of content determination, it is a special and practical quantitative analysis method. 6. By using the information database to analysis and characterize the Chinese herbal medicine and compounds of traditional Chinese Medicine, we supplied evidences for quality control of medicine and standard components, provided information supporting platform for the high-efficiency and in-depth research of traditional Chinese Medicine’s pharmacodynamic material basis and the development of components of traditional Chinese Medicine.
A-150The impact of Hypoglycemic Ziyabiti Tablet (HZT) extracts on type 2 diabetes mellitus rats and its activity in cultured liver cells
Li Guirong2, Fan Yong2, Wang Xiaofeng3, Mao Xinmin1, Yi Jingyang1, Han Xue1, Ma Xiaoli1, Wang Lifeng1, Ren Jun1, Wang Ye1*, Li Linlin1* 1Department of Pharmacology, Xinjiang Medical University, Urumuqi, 830011Xinjiang Uighur Autonomous Region China; 2First
affiliated hospital of Xinjiang Medical University, Urumuqi 830011 ， Xinjiang Uighur Autonomous Region, China; 3China Pharmaceutical University, Nanjing, China;* Correspondence should be addressed to [email protected] (Ye Wang); yz-
[email protected] (Linlin Li)
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Objective: The present study aimed to investigate the impact of Hypoglycemic Ziyabiti Tablet (HZT) extracts on type 2 diabetic rats as well as its activity in cultured liver cells. Methods: Wistar rats were given a high fat emulsion for 10 days, and their insulin resistance was assessed using a blood capillary method. Alloxan (ALX) was intraperitoneally injected for two subsequent days to induce the type 2 diabetes mellitus (T2DM) in rats. The T2DM rats were divided into 5 groups: saline (20ml·kg-1·d-1), positive control (Xiaoke Pills 420mg·kg-1·d-1) and groups treated with different dosages of HZT (220,440, and 880 mg·kg-1·d-1). Fasting blood glucose (FBG), total cholesterol (TC), total triglycerides (TG), high-density lipoprotein (HDL-C), fasting insulin in serum (FINS), insulin secretion (IS) and insulin sensitivity index (ISI) were measured. Results: HZT at 220 and 440 mg·kg-1·d-1 decreased the FBG and TC levels (P﹤0.05); HTZ at 440 mg·kg-1·d-1 and XKP (Xiaoke pills) at 420 mg·kg-1·d-1 increased the FINS level (P﹤0.01), and HZT at 220 and 440 mg·kg-1·d-1 and XKP increased IS level (P﹤0.05). The vitro studies showed that HZT at 0.1, 0.2, and 0.4mg/ml decreased the LDH activity and promoted the glucose consumption in a L-02 Par model. Conclusion: The hypoglycemic mechanism involving HZT is possibly contributed to the promotion of insulin secretion in the pancreas and increased utilization of glucose in the liver.
A-151The study on the development of the Xipayisolid-gingival buccal tablets
Ma Pingping1, Nie Jihong1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China;* Correspondence should be addressed to [email protected]
Objective: To definite the technology of preparation, establish the quality standards and proceed the preliminary pharmacodynamics studies of xipayi solid- gingival buccal tablets. Methods: 1) To determine the Gallic extraction process parameters based on the composite index tannin, gallic acid and extraction yield ; 2) To determine the Gallic purification process parameters based on the composite index tannin, gallic acid and extraction yield ;3) To research the moulde technology of xipayi solid- gingival buccal tablets;4) Employ thin layer chromatography to qualitation discrimination, using the method of HPLC to determine the contents and institute the quality criteria to the produce obtained from the followed preparation technology; 5) To evaluate its effectiveness according to the preliminary pharmacodynamics studies. Result: 1) Optimizing extraction conditions by adding 8 times the amount of water ,extracting three times, each 2.5 hours; 2) Optimizing refining process by concentrating the extraction to medicine: liquid (1:5), placed in low temperature (4 ℃) frozen 24 hours, using the clear supernatant as sample; 3) The moulde technology of xipayi solid- gingival buccal tablets was used 7 times Sugar power,10% microcrystalline cellulose, 3‰ beetsugar as supplementary material and 93% ethanol as bond, adding 6% menthol of ethanol liquid and 1% magnesium stearate to press tablets; 4) Establishing the preparation of gallic by TLC method and quality standards by HPLC, the method of gallic acid with HPLC: mobile phase: methanol - tetrahydrofuran - acetic acid - water (0.08:0.64:2:97.28) in mobile phase and the detection wavelength of 271nm, column temperature:35 ℃, the flow rate:1ml/min. ; 5) Preliminary pharmacodynamic showed that, significant inhibition roles in xipayi solid- gingival buccal tablet induced ear swelling, pain caused by heat stimulation in mice, rats phenol oral ulcers. Conclusion: The preparation technology of xipayi solid- gingival buccal tablets is stable, reasonable and feasible process. The quality standard is convenient, accurate, and can be used to
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quality control. Preliminary pharmacodynamic results showed better roles in anti-inflammatory agents, analgesic and promoting healing of mouth ulcers.
A-152Toxicological evaluation aqueous extract of the galles of Quercus infectoria olivin rodent
Mubarak1, Nurmuhammat Amat2*, Kasimjan, Halmurat Upur2*1 Pharmaceutical Department, Xinjiang Medical University, 830011-Urumqi, Xinjiang Uighur Autonomous Region, People’s
Republic of China; 2Traditional Uighur Medicine Department, Xinjiang Medical University, 830011-Urumqi, Xinjiang Uighur
Autonomous Region, People’s Republic of China；*Correspondence should be addressed to Nurmuhammat.Amat；E-mail：[email protected]，[email protected]
Objective:Galls of Quercus infectoria Olivier (Fagaceae) is one of the most popular medicinal plants in Uighur Medicine. The aqueous extract preparation of Q. infectoria galls is widely used traditionally in Uighur Medicine for treatment ulcerative colitis and hemorrhoid etc but to date there is no documented evidence corroborating its safety. The present investigation was carried out to evaluate the safety of an aqueous extract of the Q. infectoria galls in rodent by determining its potential toxicity after acute and chronic administration in rodents. Methods: For the acute study, an aqueous extract of the Q. infectoria galls was administered to ICR mice in doses (5-10g/kg body weight) given by enema. General behavior adverse effects and mortality were determined for up to 14 days. In the sub-chronic dose study, the aqueous extract preparation of Q. infectoria galls was administered rectally at doses of (5-10g/kg body weight) daily for 180 days to Wistar rats. Otherwise it has one month convalescent period after the discontinuation. Selected biochemical 、 hematological and histopathological parameters were determined after180 days. Results: In the acute study in mice, acute intake of extract up to 10 mg/kg did not cause any mice was death so disable to calculate LD50, also did not produce mortality or significant changes in general behaviour. Rats given oral doses of Q. infectoria galls-extract (10、7.5 、 5.0g/kg) survived the 6 months of dosing (i.e. LD50 much higher than 1 g/kg), experienced no significant changes in general behaviour and hematological and biochemical parameters, except for transient decrease in AST activity. No significant changes were observed in organ weights, and histopathological results showed normal profile suggesting no morphological alterations. Because of the relatively high NOAEL values in the acute study in mice, and lack of mortality or clinically significant changes in the biological and hematological parameters in rats after 180 days of daily dosing, it may be concluded that the aqueous extract preparation of Q. infectoria galls does not appear to have significant toxicity. Conclusion:In view of the doses consumed empirically in traditional medicine in Morocco, there is a wide margin of safety for the therapeutic use of Q. infectoria galls.
A-153Dialectical Analysis of exercise-induced fatigue patients according the Uighur Medicine humoral quality
Ailyjan Asla, Meng Qingcai1, Liu Zhenfeng1, Fang Rui1*1 Affilated Tradional Chinese Medicine Hospital ,Xinjiang Medical University, Xinjiang Uighur Autonomous Region, 83000,
Urumqi, China; *Corresponding to [email protected]
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The exercise-induced fatigue is a phenomenon caused by the body movement itself, "the body's physiological processes can not be continued to hold its function in a particular level and / or unable to maintain a predetermined exercise intensity" of the body decreased exercise capacity. Uighur Medical body fluids qualitative theory that the body fluids qualitative abnormalities is the main reason to cause changes in human physiology, psychology, pathology, function. That exercise-induced fatigue Uighur Medical abnormal humoral quality areas. This article focuses on exercise-induced fatigue and Uighur Medicine humoral quality links discussed from the perspective of the Uighur Medical fluid transfer theory, and exercise-induced fatigue body fluid quality type for Analysis.
A-154Comparative study Uighur Medicine sand therapy with other regional sand therapy， sand characteristics
Abudusalam Saidula1, Osman Yasin1, Satar Kurban1, D.Mahemuti 2
1Uighur Medicine Hospital of the Turpan area, Turpan, 838000, Xinjiang Uighur Autonomous RegionChina; 2Department of
Mechanical Engineering, Xinjiang University, Urumqi , Xinjiang Uighur Autonomous Region，830049, China
The mechanism of the Uighur medical sand therapy and sand characteristics are inherently tied. We have done the comparative study of the characteristics of Uighur medical sand therapy with other regional sand therapy sand in this paper. The results show that the magnetic mineral content of the sand of Turpan has more than another area sand, also the specific weight is larger, the particles of sand is relatively uniform, Magnetic ore particles is larger ,and with strong magnetic, showing the gray and black. The results of this study provide a scientific basis to further explore the mechanism of the Uighur medical sand therapy.
A-155The Relationship between toxic electron microscopy examination and alkaloid amounts in Datura stramonium L seeds of Xinjiang
R.Islam 1, Y.Mamat 1, A.Abdugheni 1,Yanming1, S.Abay 1
1Inistitute of Xinjiang Traditional Uighur Medicine, 776, YaAn Road, Urumqi, 830049, China; 2Laboratory of Traditional Uighur Medicine Prescription of Xinjiang, 776 ,Yan An Road , Urumqi 830049
Objective: To investigate the relationship between toxic Electron microscopy examination and alkaloid Amounts(atropine,scopolamine) in Datura stramonium L seeds(crude and extraction drug ) of Xinjiang. Methods: SD rats were randomly divided into solvent control group, extraction of Datura stramonium L seeds group (1.55,3.10,6.20g crude drug·kg-1),Datura stramonium L seeds crude drug group(3.10 g crude drug·kg-1) ,each group orally was given 1ml·100g-1 for 90continuous days. then to observed Electron microscopy examination of liver ,kidney ,brain and also the alkaloid amounts were tested.Results: Results showed that Datura stramonium L seeds(crude drug) all animals and extraction
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of Datura stramonium L seeds(6.20g crude drug·kg-1) half animal can cause drug-induced liver injury, and the alkaloid amounts in Datura stramonium L seeds(crude and extraction drug) were 0.2859 mg/g,0.8136mg/g;2.1178 mg/g,4.1700 mg/g. Conclusion: Datura stramonium L seeds(crude and extraction drug) can cause liver damage, but the damage degree does not direct ratio with the alkaloid amounts.
A-156Effect of psychological factors on vitiligo patients’ hypothalamus-pituitary-adrenal axis and intervention study
Aikebaier Anzhaer 1, Silafu Aibai 1*, Tuerxun Wufuer 1, Ayinuer Aimaiti 1, Aizezi Yasen 1, Guzainuer Kuerban 2, Julaiti Abuduwayiti 2, Awaguli Haibibula 3
1 Uighur Medicine Hospital of Xinjiang Uighur Autonomous Region, 830049, Urumqi, Xinjiang Uighur Autonomous
Region，China; 2Uighur medicine skin disease key subjects of State Administration of Traditional Chinese Medicine, 830049,
Urumqi, Xinjiang Uighur Autonomous Region ， China; 3Uighur medicine vitiligo diagnosis and treatment key unit of State
Administration of Traditional Chinese Medicine, 830049, Urumqi, Xinjiang Uighur Autonomous Region ， China; *Corresponding author: Sirap.Abai, Email:[email protected]
Objective: Associate and analyize anxiety and depression psychological factors involved in the occurrence and development of vitiligo, and to explore its affect on patients’ hypothalamus-pituitary-adrenal axis (HPAA), and the role of psychological interventions to reduce symptoms and improve efficacy. Methods: Different stages of vitiligo patients using the Hamilton Anxiety Scale (HAMA) and Depression Scale (HRDS) score, and using radioimmunoassay for the determination of serum CRH, ACTH, Cortisol level were used to compare with the normal control group and contrast patients who had psychological intervention to those who did not had. Results: (1) Anxiety and depression of vitiligo patients who are in advanceing and stable phase are significantly severe than normal contrast, stable phase are less severe than advanceing phase, but have no significance. (2) In the assay for the HPA axis hormone levels, stable phase patients’ cortisol levels are significantly lower than advancing phase patients’ and normal person’s, the differences are statistically significant. (3) After psychological intervention, the intervention group patients’ efficacy and HAMA, HRSD score was significantly lower than non-intervention group. Conclusion: Incidence of vitiligo is closely related to changes in patients’ Neuroendocrine-Immune network, HPA axis changes in hormone levels play an important role on the pathogenesis, including stress factors like anxiety and depression which make a variety of hormones body changes in the body, The mechanism may be as follows: Cortisol levels of vitiligo patients with high expression can induce the activity of liver tryptophan pyrrole enzymes, the latter degradation of tryptophan in the blood, resulting in the lack of central tryptophan and 5-HT synthesis decreased. Trigger depression, obsession, anxiety and other symptoms in turn to affect the tyrosine metabolism, cause or aggravate vitiligo. But that is before vitiligo or follow-up reaction of vitiligo, which remains to be studied further. Meanwhile, patients who had psychological intervention on the basis of Traditional Uygur Medicine comprehensive treatment reduced the pressure on the patients, so that the patient's quality of life improved significantly and also improved efficacy of Uygur Medicine’s comprehensive treatment.
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Arthroscopic Debridement Combined with Uygur Maizhuni Treatment of Knee Osteoarthritis andomized Controlled Trials
Yuan Guoxiang1, Lu Yong1*1 Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region，China; *Correspondence should be addressed to [email protected]
Objective: To observe the clinical efficacy of Uygur maizhuni combined with arthroscopic debridement treatment as early as the mid knee osteoarthritis and surgery before and after the medication in patients with synovial fluid and serum expression of MMP-1, 3,13 and TIMP-1 and IL-1the level of determination of Uygur maizhuni treatment as early as in advanced knee osteoarthritis may be the mechanism. Methods: 90 cases of patients with knee osteoarthritis were randomly divided into experimental group and control group, 45 cases in each group .The experimental group in arthroscopic knee clean up with 3 days after Uygur maizhuni treatment control group to be after three days of placebo, 4, 8 and 12 weeks after surgery, symptoms, signs integral, the VAS, as the value of law and security indicators to assess and detected before surgery and 12 weeks after surgery in patients with synovial fluid and serum of MMP-1, 3,13 and TIMP-1 and IL-1 level. Results: Postoperative efficacy of 12 weeks and found that the experimental group total effective rate was 97.8%, the total effective rate was 80.0%, 93.3% of the total effective rate of the control group, the total effective rate was 57.8%, statistically significant differences between the two groups(P <0.05) after the end of the treatment, the serum MMP-3, TIMP-1 in serum and synovial fluid with the control group after the treatment group compared to the difference was statistically significant (P <0.05).Conclusion: Compared with simple arthroscopic debridement, the arthroscope with the dimension drugs buy Uygur maizhuni treatment of early osteoarthritis of the knee of the medium-term efficacy is more significant, especially for patients with swollen joints, pain, joint activity, playing soft legs and other symptoms and signs improved significantly, but its mechanism of action and long-term efficacy remains to be further experimental studies and clinical observations to verify and prove.
A-158Association analysis Uygur Medicine's combined modality therapy with ordinary psoriasis patien's cytokine and immunologic mechanism
Riziwan Wumaier 1, Aikebaier Anzaer 1, Silafu Aibai 1*, Tuerxun Wufuer 1, Ainuer.Aimaiti 2, Aizezi Yasen 2
Uygur Medicine hostipal of Xinjiang Uygur Autonomous Region, 830049, Urumqi ， Xinjiang Uighur Autonomous
Region，China; The Key Subject of Uighur medicine’s Dermatology of State Administration of Traditional Chinese Medicine,
830049, Urumqi Xinjiang Uighur Autonomous Region，China; *Corresponding author: Sirap.Abai, Email:[email protected]
Objective: On the basis of previous study, unite Uygur-medicine's theory with latest disease pathologic mechanism study，observe representative cytokine of Th1 and Th2 cell in ordinary psoriasis patient’s serum and possible mechanism. Methods: Five cytokine including IFN-γ、IL-2、TNF-α及 IL-10、IL-4 of 80 ordinary psoriasis patient’s(before and after combined modality therapy of Uygur-medicine ) and 60 healthy volunteer’s peripheral blood serum are assayed by enzyme linked immunosorbent assay(ELISA) for Association analysis. Results: (1) before treatment, contrasted to the healthy
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volunteer’s, the IFN-γ、 IL-2、 and TNF-α concentration levels of ordinary psoriasis patient are higher, in which, IFN-γand IL-2 have extreme significance(P<0.001). TNF-α、 IL-8、 IL-2、 IL-6 levels are lower, which have extreme significance（P IFN-γ=0.043，PIL-2=0.008） . IL-10、 IL-4 significantly lower than the healthy contrast's; (2)after a course of combined modality therapy,IFN-γ、IL-2、TNF-α and IL-10、 IL-4 level of ordinary psoriasis patient have no significance contrast to the healthy volunteer’s, but IL-2 level still has. Conclusion: Vitiligo patients have Th1 type cytokines advantaged, CD4+T cell subgrup(Th1/Th2)unbalanced differentiation and their secreted cytokine are correlation factor of ordinary psoriasis's initiation and development, Uygur-medicine's combined modality therapy can significantly improve the unbalance status mentioned above via multiple targets.
A-159Study On Xanthine Oxidase Inhibitory Actvity In Capparis Spinosa.L By Spectrophotometry
A.Rahman 1, A.Tax 2, N.Amat 1*1Traditional Uighur Medicine Department, Xinjiang Medical University, 830011-Urumqi, Xinjiang Uighur Autonomous Region， People’s Republic of China；*Correspondence should be addressed to Nurmuhammat.Amat，[email protected]
Objective：Xanthine oxidase (XOD) inhibitor, is an important class of anti-gout drug, seek strong XOD
inhibitory activity of natural drugs and their active ingredients have great significance. In this study, a comparative study of Capparis spinosa .Ldifferent parts of different solvent extracts in vitro xanthine oxidase inhibitory activity of the active site was done to look for Capparis spinosa. Lrole of inhibition of xanthine oxidase (XOD) and to provide the further development dimension of the treatment of gout drug new drugs basis. Methods: UV spectrophotometric determination of Capparis spinosa .L different parts of different solvent extracts (cyclohexane extract, dichloromethane extract, methanol extract) inhibition of xanthine oxidase activity. Results: The Capparis spinosa L different solvent extracts of fruit ,leave and flower extracts displayed xanthine oxidase inhibitory activity. In 100mg/mL concentration, the medicine in three parts (leaves, fruits, flowers) in three different solvent extracts (cyclohexane extract, dichloromethane extract, methanol extract) to varying degrees, for XOD inhibition effect, and leaves cyclohexane extract, fruit cyclohexane extract, leaf dichloromethane extract, fruit methanol extract, flower methanol extract inhibits the rate of more than 50%; concentration of 50 mg/mL, six different solvent extracts showed inhibition and which three extracts exhibited higher than 50% inhibition rate; while three extracts presented activity at 25mg/mL,with none having greater than 50%inhibition. The most two active extracts were the fruit cyclohexane extract ,leaves cyclohexane extract,with IC50 value of122.50mg/mL,127.66 mg/mL. Conclusion: The fruits of cyclohexane extract, leaves cyclohexane extract is activity part of Capparis spinosa L medicinal plants inhibition of xanthine oxidase, can be further purified the active compound.
A-160Clinical effecacy observation Uighur Medicine Preparetion Denar on the patients with viral hepatitis with Cholestatic syndrome
Dmytriy SebrebryakovTurkministan State Medical University, Ashkabad, Turkministan
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The treatment of cholestatic forms of viral hepatitis is the one of the complicated question in hepatology, the purpose of this reasearch is application of the Uighur Medicine Preparetion Denar decoction treatment the hepatitis with Cholestatic syndrome, Denar decoction can reliefe the fever of the liver, remove toxin, remove sputum, functionging as a choleretic , laxative and uriginative agent. There was 39 patients with chronic viral hapatitis with cholestatic sysndrome was under observation. The 15 of them were with chronic viral hapatitis HBV+HDV, 18 with the chronic viral hapatitis HBV+HCV, and 6 with the chronic viral hapatitis HBV.The diagnosis was verified by determination of infection marker of viral hepatitis.Along with the basis treatment the “Denar ” was prescribed three times a day by 3-5g deluated with warm water.The course of therapy consisted of 21 days.The control group conisited of 20 patients with the cholestatic form of chronic viral hepatitis during the basic therapy.The effeciency of therary was evaluated by laboratory data ,there was noted amelioration during the usage of “Denar” .Many of the patients faster then in control. Lowering of the skin itch intensity, normalize sleeping. During the treatment , Ictertitiousness of the coverlet sypntom of 30 of patients had decreased or then disappeared ,and fast reverse dynamics of biochemical tests, for the 28 patients the biochemical markers was improved ,also correlated with immune parameter was normalized. 9 patients at the end of treatment had the marker of the viral hepatitis decreased or show negative (superficial antigin of viral hepaptitis HBV, antibodies IgM to the heart shaped antigen of viral hepaptitis HBV in the immune-enzyme analysis) and there was no datected nucleic acids of viruses in polymerase chain reaction. We also found that can not find any side effection and allergic affection. Thus, the inclusion to the complex therapy preparation “Denar” allowed to get convincing clinical effect. The obtained results in application of this medicine in treatment of viral hepaptitis with cholestatic syndrome syndrome present itself very prespective.
A-161Clinical Observation of Wujing Ointment in Reducing Adverse Reactions attached to Asthenia of Spleen and Pathogenic damp of FOLFOX4 in Treating Cancer of Colorectum
Xiao Chunxia 1, Zhang Hongliang 1,2*1Affilated Traditional Chinese Medicine Hospital,2Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region ， China; *Correspondence should be addressed to [email protected]
Objective：To observe the clinical effects of Wujing Ointment in educing adverse reactions and initially explore its mechanism of action by treating the patients of Cancer of Colorectum attached to Asthenia of Spleen and Pathogenic damp with FOLFOX4. Methods：76 cases who meet the diagnosis standard of Cancer of Colorectum from our co-operation hospitals were domized into two groups (38 cases respectively)：the control group which FOLFOX4 only was given and the treatment group used Wujing Ointment at the same time.，60g/d，three times a day. After one cycle, we surveyed the quantity and the descenting rate of WBC、the quantity and the descenting rate of Hg、 incidence rate of nausea and vomit、the changes of syndromes of TCM、Karnofsky scores、immunity and completed rate of chemotherapy.Results： the WBC quantity of two group decreased after chemotherapy ， the descenting rate of WBC of the treatment group is lower than the control group，and the difference was significant statistically（P＜0.05）；The descenting rate of Hg of two groups and the degree has no statistical difference (P＞0.05). The syndromes of TCM of two groups all increased after chemotherapy
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(P＜0.05).and there was different statistically (P＜0.05)；The Karnofsky scores of two groups all increased after chemotherapy (P＜ 0.05).and there was a statistical difference (P＜ 0.05)； The immunity function after chemotherapy were higher than the before,and the differences were significant statistically (P<0.05). The completed rate of chemotherapy of two groups was higher than 85%，and there was no different(P＞0.05).Conclusion：1.Wujing Ointment can boost patients′ immunity after FOLFOX4 and can elevate CD4、CD4/CD8、NK，and reduce CD8；Wujing Ointment can lower the descenting rate of WBC；Wujing Ointment can improve the patients′ KPS.2. Wujing Ointment can improve the TCM clinical symptoms of asthenia of spleen and pathogenic damp due to chemotherapy and improve the patienrts′ quality of life.
A-162Clinical research of auxiliary therapy effect of Turnips on tuberculosis patients
A.Pattar 1, Nurmuhammat Amat1，Halmurat Upur1*1Public Health Institute, Nutrition Department, Xinjiang Medical University, Urumqi, 830011 ， Xinjiang Uighur Autonomous Region; 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region, China; *Correspondence should be addressed to [email protected]
Objective: Brassica campestris L. is cruciferous brassica roots of plants. One of traditional Uighur Medicine for lung disease drug used along with one of the plant resources of food, widely planted in the northwest of the Tarim Basin, Xinjiang, The Qmagur (Referred to as QMG) is Uighur Medicine name of Brassica campestris L. The QMG both into the dishes, they can do the diet, people in the past two thousand years accumulated a wealth of treatment, health care knowledge and methods. A Tuberculosis (TB) is caused by Mycobacterium tuberculosis and malnutrition-related infectious diseases, tuberculosis, once widespread in the world, especially the economically backward, living in poor areas. Among TB patients and 88.6% with malnutrition, malnutrition is the lack of calories and protein caused by a nutritional deficiency. The number of TB patients in China ranks second in the world, which is one of the world's 22 TB high-burden countries. Smear-positive prevalence and mortality in patients with active pulmonary tuberculosis morbidity in Xinjiang are 1.3 times the national average, 1.5 times and 3.9 times, especially the southern border region is more prominent. In short, the TB epidemic in Xinjiang is still very severe, an increasingly large number of patients, the huge cost of treatment and prevalence of low quality of life has become a social burden, therefore tuberculosis has become the most important public health problem in Xinjiang. In order to clarify the QMG on the role of adjuvant therapy for patients with pulmonary tuberculosis, we screened for tuberculosis patients for nutritional intervention studies. Methods: Pulmonary TB nutrition intervention: nutrition intervention study screening patients 137 patients were divided into control group and intervention group. The intervention group was given the QMG honey cream 3, observed changes in the indicators between the two groups before and after the intervention. QMG honey paste on immune function: CP induced immunocompromised animal models of nutritional intervention. Results: The results showed that pulmonary TB patients after the intervention of weight and BMI were significantly higher (P<0.01), cough, sputum, fever, night sweats, loss of appetite and weight loss and other symptoms improve (P<0.01 and P <0.05), X-line and sputum smears, etc. have disappeared. Blood biochemical indices, HGB, of ESR, ALB, GLO, ALT, and AST indicators into a normal number of significant increase (P<0.01 and P<0.05). Results showed that QMG can significantly improve the CP-induced mouse thymic and
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splenic atrophy, plus ConA stimulated mouse spleen lymphocyte proliferation rate increased significantly (P<0.01); increase in thymus, spleen suspension and serum white interleukin 2 - (of IL-2), of γ-interferon (of IFN-of γ) the secretion (P<0.01); can improve of CD4 +, of CD8 + cells of the The number and CD4/CD8 ratio (P<0.01 and P<0.05), is capable of regulating the dynamic equilibrium of the T lymphocyte subsets. Conclusion: QMG honey cream rich in protein, vitamins and other nutrients, to meet patients' physical needs and disease consumption, to improve the nutritional status of pulmonary TB patients, reducing the side effects of chemotherapy drugs, improve immunity and protect the liver and kidney function, so improve the efficacy of adjuvant therapy. The rapid development of great significance and broad application prospects for the future development of other natural resources, nutrition and treatment, non-toxic side effects of special nutritional food, to carry out effective prevention and control of tuberculosis and to speed up the economy of the west.
A-163Clinical Therapeutic Efficacy and Security Assessment of Uighur medical treatment for Vitiligo with Uighur Medicine
Tursun Wuphour *1, Yüsup Metenur 1, Silafu Aibai 1*, Ëziz Yasen 1, Shewket abdula 2, Roshen gül Shawuer 3, Aixirejiang Sidike 3, Erkin Turdi3 ,Qurban Eli 4, Yitaipaer mulate 1, Mubareck Paizula 1, Huzanur Qurban 1, Yilikaer Baiketiya 1, Julaiti Abduwayiti 1, Zaitungül Wuphour 1, Ekber Anzhaer 1, Refuhati Saimaiti 1
1 Xinjiang Uighur Autonomous Region Hospital of Uighur Medicine; 2Kashi Hospital of Uighur Medicine; 3Hotan Hospital of
Uighur Medicine; 4Korla Hospital of Uighur Medicine, China; *Corresponding author: Silafu · AIbai Email: [email protected] ;
Tursun·Wuphour Email: [email protected]
Objective: Evaluate the clinical therapeutic efficacy and safety of treating vitiligo with Uighur Medicine. Method: treat leukoderma patients with the Uighur medical treatment, as well as abnormal mucilaginous maturing agent and scavenger, Zibifu Sugar Liquor, Vitiligo Ointment, Kali Injection, Compound Kali Tablet, Yi Ti Re Fei Li Ai Ke Mu Ai Li, Vitiligo Liniment, insolation and ultraviolet therapy, and conduct therapeutic efficacy and safety evaluation before, after and during the treatment. Result: No significant difference (before the treatment) of white spot total area to the one during the treatment: Z=0.42, P=0.68; significant difference (before the treatment) of white spot total area to the one after the treatment: Z=3.91**, P<0.01; significant difference (before the treatment) of pigment regeneration total area to the one during the treatment: Z=10.2**1, P<0.01; significant difference (before the treatment) of pigment regeneration total area to the one after the treatment: Z=17.63**, P<0.01. The patients suffer from no severe safety problem or adverse reaction. Conclusion: Uighur medical treatment with Uighur Medicine has actual therapeutic efficacy and perfect safety.
A-164Correlation Analysis of Syndrom Differentiation of Vitiligo and Its Munzich and Mushil Therapy Based on Traditional Uighur Medicine Theory
Silafu Aibai 1, Tuerxun Wufuer 1*, Aizezi Yasen 1, Abudoujili Abudouaini 1, Zaituguli Wufuer 1, Guzainuer Kuerban 1, Julaiti Abuduwayiti 1, Aikebaier Anzaer 1, Refuhati Saimaiti 11Xinjiang Uygur Traditional Medicine Hospital, 830049, Urumqi China; The Key Disciplines of Uygur Medical Dermatology of
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State Administration of Traditional Chinese Medicine, 830049, Urumqi, China,* Corresponding author: Tuerxun Wufuer
Objective: In this study, 341 cases of vitiligo patients in line with the inclusion creteria were conducted syndrome differentiation typing analysis on admission. Risk factors, the original Mizaj (temperament) and abnormal Hilit distribution, factors affecting the efficacy, the top ten symptoms that most intensively associated with the syndrom differentiation type were analysed. On this basis, quantitative assessment were conducted on white spots and pigment regeneration area and the symptoms before and after the use of Munzich and Mushil. And with multivariate statistical analysis, were analysed the factors of morbidity and induced vitiligo and affecting the efficacy in order to lay the foundation for the standardization of disease diagnosis of abnormal Hilit while correlation analysis were conducted between correlation of Munzich and Mushil therapy to restore the vitiligo systemic pathological changes. Results: Patients with Hol Sohuq Mizaj (wet cold temperament) accounted for 32.8%, with Kuruk Issik Mizaj (dry and hot temperament) accounted for 29.9% of the, with Hol Issik Mizaj (hot and wet temperament) accounted for 7.3%, with Kuruk Sohuq Mizaj (dry and cold temperament) accounted for 29.9%. Eating habits in the risk factors accounted for 95.9%, followed by mental factors as 9.4%, and Moza Temlik Bilghem(astringency phlegmatic) accounted for 34.6% in healing Hilits. These are consistent with the premise research project Retrospective Analysis of Uighur Medical Diagnosis of Vitiligo by this research team. Compared with the time of admission, the white spots mean total area of patients has significantly narrowed (P <0.01) after the use Munzich and Mushil, leukoplakia district obvious pigment regeneration (P <0.01), symptoms improved significantly ((P <0.01). Conclusion: Due to the incentives such as eating habits and mental factors,patients with Kuruk Sohuq Mizaj (dry and cold temperament) and Hol Sohuq Mizaj (wet cold temperament) phlegm in the body gradually increases and goes beyond the body's self-regulating capacity led to vitiligo. The Moza Temlik Bilghem (astringency phlegmatic) vitiligo is relatively common according to the original temperament reasons.This matches one of the typical Uygur Medicine classic "Mehzanul Edwiye" records: Moza Temlik Bilghem (the astringency phlegm) at the same time has the characteristics of excessive humidity and excessive cold, one of typical abnormal Hilit, so that vitiligo is most common.Munzich and Mushil Therapy mainly through mature and remove abnormal Belghem Hilit (phlegm) and regulate the body environment, strongthen natural forces to control the disease progresses. Avicenna, a famous ancient doctor in the 11th century, in his book Medical Code, and Muhammad Akbarali in 1750 AD in his book Tibbi Akbar recorded: Vitiligo is caused by strong concentrated phlegm,by which the transformation force and the exclusion force of the body weakened,so that the metabolic productions of the body cannot be excreted properly, and cause the vitiligo lesion to occur. Munzich and Mushil therapy is to correct abnormal temperament, to promote the generation of normal Hilits（body fluids） and restore the body's natural forces to control the proliferation of leukoplakia. The result of this research is consistant with the records. The results of this research proved the above points of ancient scholars from the point of view of modern medicine combined with Traditional Uighur Medicine theory.
A-165Detection of neuroendocrine and immune factors before and after traditional Uighur medicine treatment of vitiligo and it’s significance
Aikebaier Anzhaer 1, Silafu Aibai 1*, Tuerxun Wufuer 1, A.Aimaiti 1, Aizezi Yasen 1, G.Kuerban 2, J.Abuduwayiti 2, A.Haibibula 2
1 Uyghur Medicine Hospital of Xinjiang Uighur Autonomous Region , 830049, Urumqi, China; 2Uighur medicine skin disease
key subjects of State Administration of Traditional Chinese Medicine, 8300493, Urumqi, China; Uighur medicine vitiligo
diagnosis and treatment key unit of State Administration of Traditional Chinese Medicine, 830049, Urumqi , China,
*Corresponding author: Sirap.Abai, Email:[email protected]
Objective: Explore the relationship between vitiligo and the development of neuroendocrine-immune network‘s environmental change of patients, combined with the Uighur medical theory, explore its relationship with vitiligo. Methods: Before and after receiving Uighur medicine’s treatment, 200 Vitiligo
patient’s blood serum are assayed for IFN-γ，sICAM-1，5-HT，IgG、Cortisol， NE，ACTH，IL-
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10 ， MT ， EP-β ， CRH and E level by enzyme linked immunosorbent assay (ELISA). Results:
（1）Following index :IFN-γ、NE、ACTH、IgG、5-HT、IL-10、sICAM-1、EP-β、E, have significant
difference compared with those before treatment. IFN-γ、NE、ACTH、5-HT、IL-10、EP-β、E are tends to
normal control level. Conclusion: Those results indicates that the invasion of Vitiligo is a comprehensive course of neuroendocrine-immune network, which corresponds with the Uighur medicine’s theory that imbalanced “kuwiti tabiiy” [“Nature power”(of human body)] induces Vitiligo. Traditional Uighur medical treatments can improve the environment within the neuroendocrine-immune network of patients with vitiligo.
A-166Discussion on the relationship between vitiligo and peripheral blood in traditional Uighur Medicine
R.Umar 1, J.Abduwayet 2, T.Tursun 3
1Xinjiang Uighur autonum region Uighur medicine hospital, 830049 ,Urumqi, Xinjiang Uighur Autonomous Region ， China; 2Xinjiang Uighur autonum region Uighur medicine hospital, 830049 ,Urumqi, Xinjiang Uighur Autonomous Region ， China; 3Xinjiang medical university traditional Uighur medicine institute, 830054, Xinjiang Uighur Autonomous Region，Urumqi, China
Objective: Discussion on the relationship between vitiligo and peripheral blood. Methods: 100 cases of skin clinic, hospital vitiligo patients and In 100 patients the hospital health care branch to attend the examination of normal people were peripheral blood detected (Red blood cells and its classification、 white blood cell, and its classification). Result: And compared with the normal group and patients group with vitiligo RBC、HGB、HCT、MCV、MCH、MCHC reduce (P<0.05), RDW-SD rise (P<0.05). Conclusion: Compared with normal subjects, patients with vitiligo have differences in peripheral blood, the red cells and its classification is obviously different.
A-167Effect of Euphorbia humifusa active component on the cell membrane biosynthesis and gene expression of metalloprotease(MEP), subtilisin(SUB) of Trichophyton rubrum
Li Zhijian 1,2, Zhao Ming yue 1, An Huixia 1, Gulnar Dawuti 3, Silafu Aibai 1,2,3*1Xinjiang Institute of Traditional Uighur Medicine, Urumqi 830049, Xinjiang Uighur Autonomous Region ， China； 2Key
Laboratory of Traditional Uighur Medicine Prescription, Urumqi 830049, Xinjiang Uighur Autonomous Region ， China；3Uighur Medicine Hospital of Xinjiang Autonomous Region, Urumqi 830049, China, *Corresponding author: Silafu
Aibai, ,Professor, Doctoral supervisor, Email:[email protected]. Author:Lizhijian, Research Assistant,
Email:[email protected].
Objective: To study the effect of Euphorbia humifusa active component(EHAC) on CYP51,lanosterol biosynthesis, ergosterol biosynthesis and the expression of gene metalloproteinases(MEP) and subtilisins(SUB) in Trichophyton rubrum, and explore the potential mechanism of their antifungal activity. Methods: Treated T.rubrum with EHAC, the effects of EHAC on CYP51 studied by ELISA test,
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the content of lanosterol and ergosterol in mycelium of Trichophyton rubrum detected by reversed phase high performance liquid chromatography, and total RNA of Trichophyton rubrum was extracted for semi-quantitative RT-PCR to detect the expression of MEP and SUB．Results: EHAC with 32 μg/ml could inhibit CYP51 activity and the expression of gene MEP and SUB in Trichophyton rubrum (p<0.01),and accumulate lanosterol biosynthesis and decrease the content of ergosterol (p<0.01) with a dose-dependent manner during 32～128 μg/ml in dermatophyte. Conclusion: EHAC can inhibit CYP51 enzyme activity in Trichophyton rubrum, leading to accumulation of lanosterol and inhibit on ergosterol biosynthesis, and inhibit dermatophyte cell membrane biosynthesis thereby. Also, EHAC can inhibit the expression of MEP and SUB in dermatophyte, influent getting nutrition during infection process and narrowing invasion.
A-168Effect of Uighur Medicine Maizhuni on the Expression of MMP-1, MMP-3, MMP-13, TIMP-1, IL-1β, and Aggrecan in Rat Osteoarthritis Models
Zhang Yaowu1, Meng Qingcai1,2*1Affilated Traditional Chinese Medicine Hospital, 2Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region，China; *Correspondence should be addressed to [email protected]
Objective: To establishe the OA model, To investigate the expression of matrix metalloproteinase1, 3, 13, Interleukin-1βand tissue inhibitor of matrix metalloproteinase 1 in articular cartilage and the intervention effects of Uighur medicine Maizhuni. Methods: A total of 40 healthy male SD rats were randomly divided into Maizhuni group and model group，shamed operation group and normal control group. Rats were treated with Uighur medicine Maizhuni or saline by gavage in the 2nd week after model construction for 4 weeks. Results: The model of OA animal model was successfully established. Compared with model group, the degree of rat knee joint cartilage degeneration in Maizhuni group was alleviated, The cartilage general score and Mankin score in Maizhuni group was lower than that in the model group (P < 0.05), The score of Maizhuni group was higher than in the shamed operation group and normal control group(P < 0.05)，there are no differece beteeen normal control group and shamed operation group(P>0.05) ,The expression of matrix metalloproteinase1,3,13 in knee joint chondrocytes of Maizhuni group decreased than that of the control group(P < 0.05) ； The expression of interleukin1βin knee joint chondrocytes of Maizhuni group also decreased than that of the control group(P < 0.05);The expression of Aggrecan in knee joint chondrocytes of Maizhuni group increased than that of the control group;The expression of tissue inhibitor of matrix metalloproteinase 1 in Maizhuni group increased than that in the model group (P < 0.05). Conclusion: These findings indicate that Uighur medicine Maizhuni may protect articular cartilage by down-regulating the expression of matrix metalloProteinase 1, up-regulating the expression of tissue inhibitor of matrix metalloproteinase 1, reduce the expression of interleukin-1β.
A-169Research of Quality Control about Abnormal Savda Munziq Granular andα-Glucosidase Inhibitors
Xin Lude 1, Tian Shuge1, Halmurat Upur2*
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1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region，, China; 2Traditional Uygur Medicine Institute, Xinjiang Medical University, 393 Medical University Road, 830011, Urumqi ,
Xinjiang Uighur Autonomous Region，China；* Correspondence should be addressed to [email protected]
Objective: The qualitative and quantitative study of main active ingredient in Abnormal Savda Munziq Granular and the research of α-glucosidase inhibition, It Provides a stable and reliable method for the quality control of Abnormal Savda Munziq Granular, providing corresponding research data for Abnormal Savda Munziq Granular’s clinical application and development. Method: Using thin-layer chromatography (TLC) screening and qualitativethe main active ingredient of the Abnormal Savda Munziq Granular; Using the high performance thin layer chromatography (HPTLC) method for the determination of the content of rutin, rosemary acid, glycyrrhizic acid, Liquiritin; Using high performance liquid chromatography method (HPLC)for the determination of rutin, using High Resolution Rapid Resolution Liquid chromatography method RRLC determines the content of rosmarinic acid, glycyrrhizic acid, liquiritin; using the part of the extract of the four Abnormal Savda Munziq Granular, which is Petroleum ether, ethyl acetate, ethanol, water has a test on α-glucosidase inhibitory activity and examined by Bio-luminescence photometer. Results: Using ultrasonic extraction, determines Rosmarinic acid and rutin in Abnormal Savda Munziq Granular qualitatively and quantitatively with ethyl acetate - formic acid - acetic acid-water (15:1:1:1.5) as developing solvent; ethyl acetateacetate - formic acid-acetic acid-water(15:1:1:1.7) as the developing solvent determines glycyrrhizic acid and liquiritin in Abnormal Savda Munziq Granular qualitatively and quantitatively , The test sample with the standard, or control medicine’s chromatography spots have the same color and Rf values, Negative control has no significant interference,there are no large differences about rosmarinic acid, rutin, licorice glycoside, glycyrrhizic acid’squantitative results with Liquid chromatography; water part of the extract of Abnormal black Munziq has a good α-glucosidase inhibitory activity, Petroleum ether extract part has no inhibitory activity. Conclusion: The topic using TLC, HPTLC, HPLC, RRLC determines Rosmarinic acid, rutin, licorice glycoside, glycyrrhizic acid in Abnormal black Munziq qualitatively and quantitatively, using bio-luminescence photometer determines four extraction sites of Abnormal black Munziq ‘s inhibition for Α-glucosidase enzyme activity, The method specificity, simple, accurate, can be used for the Abnormal black Munziq’s qualitative and quantitative identification , also provides a reliable method for quality control of the abnormal black Munziq.
A-170Retrospective Survey and Analysis on Ulcerative colitis Treated with Uighur Medicine
Turgun Amar 1, Jurat Tohti 2, Sirap Abai 3*, Ahmatjan Mamat 4, Yusup Matnur 5, Ruxangul Sawur 6, Xawkat Abdulla 7, Ubul Asan 8, Kurban Ali 9
1,2,8Xinjiang Uighur medical school and it’s affiliated hospital, 848000, Hetian, Xinjiang Uighur Autonomous Region，China; 3,4,5
Uighur medical hospital of Xinjiang Autonomous region, 830049, Urumqi, Xinjiang Uighur Autonomous Region，China; 6Hotan
Uighur medical hospital , 848000, Hetian, Xinjiang Uighur Autonomous Region， China ;7Kashghar Uighur medical hospital,
844000, Kashi, Xinjiang Uighur Autonomous Region，China,9Kurle Uighur medical hospital, 841000, Kuerle, Xinjiang Uighur
Autonomous Region，China, *Corresponding author: Sirap.Abai, Email:[email protected]
Objective：Review the 10 years of Ulcerative colitis clinical materials of five Uighur medical hospitals
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in Xinjiang, summarized and analyzed it’s distribution in curative methods and principles, drug type,
drug use principles, non-drug treatment, Efficacy judgment and nursing measures etc. Methods：Retrieve the 180 Ulcerative colitis cases which hospitalized by the Uighur medical hospital of Xinjiang Autonomous region, a total of 5 Uighur Medicine Hospital since 1998 to 2008, conduct a retrospective study and analysis on the therapeutic, nursing materials and conclude the clinical data of these cases .
Results：Among all, 180 cases reach into the standard, so conduct a classified statistics on their
treatment process. Among all, 40 cases recovered (22.22%), 38 cases effectual(21.11%),67 cases
vali(37.22%), 29 cases invalid (16.11%), 6 cases in stable condition (3.34%). Conclusion：Simply
occur in patients with ulcerative colitis, as have different curing, nursing measures and food
taboos，which expresses traditional factors of individualized therapies. But the hospital examination
conditions, treatment of blind and without Uighur medicine for the treatment of serious phenomenon. According to patients with a history of length, they appear different clinical curative effect.
A-171Retrospective Survey, Research and Analysis on Osteoarthritis of the Knee treated with Uighur medicine
Jurat Tohti 1, Turgun Amar 2, Sirap Abai 3*, Ahmatjan Mamat 4, Yusup Matnur 5, Ruxangul Sawur 6, Xawkat Abdulla 7, Ubul Asan 8, Kurban Ali 9
1,2,8Xinjiang Uighur medical school and it’s affiliated hospital, 848000, Hetian, Xinjiang Uighur Autonomous Region，China; 3,4,5
Uighur medical hospital of Xinjiang Autonomous region, 830049, Urumqi, Xinjiang Uighur Autonomous Region，China; 6Hotan
Uighur medical hospital , 848000, Hetian, Xinjiang Uighur Autonomous Region，China ;7Kashghar Uighur medical hospital,
844000, Kashi, Xinjiang Uighur Autonomous Region，China,9Kurle Uighur medical hospital, 841000, Kuerle, Xinjiang Uighur
Autonomous Region，China, *Corresponding author: Sirap.Abai, Email:[email protected]
Objective: Review the 10 years of Osteoarthritis of the knee diagnosed materials of five Uighur medical hospitals in Xinjiang, summarize and analyze the treatment methods and principles of Osteoarthritis of the Knee, and the methods and principles of Traditional Uighur Medicine. Methods: Retrieve the 500 Osteoarthritis of the Knee cases which diagnosed by the Uighur medical hospital of Xinjiang Autonomous Region, the affiliated hospital of Xinjiang Uighur Medical School, the Uighur medical hospitals in Hotan, Kashghar and kurle provinces since 1998 to 2007, conduct a retrospective study and analysis of the 500 cases on the therapeutic, nursing and treatment evaluating materials and conclude the clinical data of these cases. Results: Among all 500 cases 410 cases reached into the standard upon conducting a classified statistics on their diagnosed process with Traditional Uighur Medicine, nursing and treatment evaluating methods. By screening the 410 standard cases, 269 cases (65.61%) have discharge diagnosis, and there is no discharge diagnosis in the medical records of the other 141 cases (34.39%). Among those 269 cases with therapeutic evaluation 4 cases (0.98%) were cured, 136 cases (33.17%), were significantly effective, 121 cases (29.51%) were effective, 6 cases (1.46%) were not effective, 2 cases (0.49%) were in stable condition. Conclusion: As the causes of Osteoarthritis of
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the Knee the Hilit Ghayri Tabiyi(Abnormal body fluids) and the mizaj(temperament) of the patients can differ. Thus different curing, nursing measures and diet prohibits measures are required, which expresses traditional factors of individualized therapies. The effectiveness of the treatment differs according to the length of the disease, the differences of the Hilit type as causing factor, and differences in principles and methods while treating. The proportion of significantly effective and the effects are higher in this research.
A-172Studies on Anti-Aphthous Ulcer and Mechanisms of Galla Turcica Extract treating Recurrent Aphthous Ulcer in experimental animals
Fan XiaoHong 1, Li Zhijian 1,2, Silafu Aibai 1,2,3*1Xinjiang Institute of Traditional Uighur Medicine, Urumqi 830049, Xinjiang Uighur Autonomous Region ， China； 2Key
Laboratory of Traditional Uighur Medicine Prescription, Urumqi 830049, Xinjiang Uighur Autonomous Region ， China；3Uighur Medicine Hospital of Xinjiang Autonomous Region, Urumqi 830049, Xinjiang Uighur Autonomous Region ， China; *Corresponding author: Silafu Aibai, Professor, Email:[email protected].
Objective: Observe the effect of Galla Turcica extract(GTE) on oral ulcer and initially investigate its possible mechanism. Methods: Models were duplicated through phenol cauterization in oral cavity. Oral mucosa protein and other matter extracted from experimental rabbits were applied to immue rats as antigen，Antigen above added adjuvant was injected to intraderm adjacent the rats spine once per two weeks to observe the occurrence and cicatrization of oral ulcer in the experimental rats, Anti oral mucosa antibody in rats bled serum was detected, and histopathology examination was performed with oral ulcer mucosa. To study the anti-inflammatory mechanism of GTE, ulcer area, congestion and recovering time, Activity SOD、MDA in rats bled serum was detected. The effect of the concentration of GTE on cell viability was evaluated using MTT and Trypan blue. Investigated on macrophage cells (RAW264.7) were determined by using neutral red assay. The supernatant was transferred into 96-well ELISA plate and then TNF-α concentrations were determined using commercial ELISA kits according to the manufacturer’s instruction. RT-PCR analysis for COX-2、 TNF-α and iNOS mRNA expression. Results: GTE relieved mice body writhing response induce by ethanoicacid, Compared with the normal control group (P<0.01). GTE decreased ulcer area(p<0.05), relieved congestion (p<0.05)and in rat model induced with phenol.All of the experimental rats developed oral ulcer with oral mucosa protein as antigen, which were anti oral mucosa antibody positive in rats blood serums. There was the significantly difference between oral mucosa protein group and deposit group (p<0.01),it also decreased recovering time (p<0.05). GTE could inhibit the mouse ear edema induced by xylene, inhibit permeability of capillaries in abdomen of mouse due to acetic acid, inhibit the rat swelling induced by carrageenin, inhibit cotton ball granuloma of rat. GTE markedly inhibited TNF-α release. It also suppressed mRNA expression of iNOS、COX-2 and TNF-α in dose-dependent manners. Conclusion: GTE can promote the healing of oral ulcers in rats, reduce the incidence of oral ulcers immunity, reduce the local inflammatory response, enhance immune function and play a role of analgesia in mouth. So we speculate that the GTE may treat the oral ulcers by the combined effects of all of the above channels. Besides, the GTE can inhibit LPS stimulating the expression of the RAW264.7 cells’ pro-inflammatory cytokines, which may be one of anti-inflammatory mechanisms of action of GTE.
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A-173Study of in vivo - in vitro Anti- Influenza virus and Anti-inflammation and immune function of Artemisia rupestris extract
Meng Fanlong 1, Li Zhijian 1,2, Silafu Aibai 1,2,3*1Xinjiang Institute of Traditional Uighur Medicine, Urumqi 830049, China; 2Key Laboratory of Traditional Uighur Medicine
Prescription, Urumqi 830049, China; 3Uighur Medicine Hospital of Xinjiang Autonomous Region, Urumqi 830049, China; *
Corresponding author: Silafu Aibai, Uighur, Professor, Doctoral supervisor, Email:[email protected].
Objective: Study of in vivo - in vitro Anti- Influenza virus and Anti-inflammation and immune function of Artemisia rupestris extract(AR). Methods: The experiment use a method that AR at the different dose add into the cells be infected by influenza virus, to observe and note the cytopathic effect (CPE) by inverted microscope and calculate the EC50,to evaluate the effect of AR against kinds of virus. We used the model that mouse be infected by influenza virus type A(FM1 strain) to observe anti-influenza virus effect of AR in vivo, used inflammatory models with xylene-induced mouse ear swelling test, increased of permeability of capillaries in abdomen of mouse due to acetic acid, injected carrageenin into the toe of rats, cotton ball granuloma of rats to study the effect of AR on aute and chronic inflammation, and used immune models with delayed hypersensitivity test, phagocyte swallow neutral red test, T,B lymphocytes increasing test, the production of serum hemolysin of mouse to study the effect of AR on immune reaction. Results: The EC50 of AR on H3N2, H1N1, BV39 were 143.14 μg/mL,333.33 μg/mL,111.11 μg/mL. AR under the 300 mg kg﹒ -1,the lung index and lung index inhibition ratio of AR on FM1 strain infect mouse respectively are 1.20±0.35、 48.72%,compared with model control group,AR have the significant difference. AR could inhibit the mouse ear edema induced by xylene, inhibit permeability of capillaries in abdomen of mouse due to acetic acid, inhibit the rat swelling induced by carrageenin, inhibit cotton ball granuloma of rats,decrease inflammatory exudate PGE2. AR could not inhibit the delayed hypersensitivity induced by DNCB,but could increase the index of thymus and spleen.AR could increase the number of T,B lymphopiesis,increase the production of serum hemolysin of mouse,activate macrophages’phagocytosis. Conclusions: AR have obvious effects on influenza virus in vitro and in vivo. It could increase the number of T,B lymphocyte,increase the production of serum hemolysin of mouse, activate phagocytosis of macrophage, inhibit acute inflammation, subacute inflammation,chronic inflammation.A-174The Nymphaea alba with oxidative damage protection to human umbilical vein endothelial cells
Lan Wei1, Halmurat Upur1*1Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, Urumqi, 830011, China;1*Corresponding authors: Halmurat Upur, Traditional Uighur Medicine Department, Xinjiang Medical University, 830054 Urumqi, Xinjiang Uighur
Autonomous Region，China, [email protected]; tel +86-991-4362206 Fax: +86-991-4361554
Objective: Screening Nymphaea alba with oxidative damage protection to human umbilical vein endothelial cells. Methods: With the concentration of 50 μg • mL-1 sample for 4 h to cells, then using H2O2 treated it for 20 h to cause the cells to oxidative damage model, using MTT method to determine the activity of the mitochondria vascular endothelial cells in order to observe samples of H2O2 endothelial cells of oxidative damage caused by the protection. Results: The Nymphaea alba
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extractings protection rate is 58.58%, and the EC50 is 19.22μg • mL-1, indicating that the Nymphaea alba extractings have protection to human umbilical vein endothelial cells oxidative damage. Conclusion: This study show the Nymphaea alba can be used for future cardiovascular new drug development to provide the evidence.
A-175Uighur Medicine Capparis spinosa treatment of 67 Cases of chronic arthritis
Hupurjan Obul11The second poor hospital of Xinjiang Uighur Autonomous Region, 830011,Urumqi，Xinjiang, China
Objective: To observe the efficacy of the treatment of chronic arthritis Uighur Medicine Capparis spinosa. Methods: 92 cases of chronic arthritis patients were divided into two groups, namely, treatment and control groups. For the treatment group, there were 67 cases of chronic arthritis patients, according to the cause of abnormal Hilit Munziq agents, using painkillers, anti-inflammatory drugs, swelling of the drugs and drugs to improve joint function, and using the Capparis spinosa medicinal bath therapy to achieve better effect. For the control group, there were 25 cases of chronic arthritis patients, according to the cause of abnormal Hilit, using Munziq agents, mature agent, analgesics, anti-inflammatory drugs, swelling of the drugs and drugs to improve joint function. Results: 67 cases of patients with chronic arthritis patients cured, 34 cases, accounting for 51%, a significant effect of 22 cases, accounting for 33%, effective 8 cases, accounting for 12%, 3 cases (4%), the efficacy of 96%. For the control group, 25 cases of chronic arthritis patients, 10 cases recovered, accounting for 40%, a significant effect of 7 cases accounted for 28%, effective four cases, accounting for 16%, 4 cases, accounting for 16%, 84% efficacy. The difference between the two groups was statistically significant (p <0.01).Conclusion: The treatment of chronic arthritis, according to the cause of the abnormal Hilit Munziq agent and use of painkillers, anti-inflammatory drugs, swelling of the drugs and drugs to improve joint function, after using the Capparis spinosa medicinal bath therapy has better effect than in the control treatment group, and the treatment time is shorter with lower costs.
A-176Uighur Medicine Karapxa decoction as a hypouricemic agent: reduces serum uric acid level, inhibits liver xanthine oxidase in hyperuricemic mice model and, scavenge free radicals in vitro
Nurmuhammat Amat1, Palidan Wushuer2, Halmurat Upur1*1Traditional Uighur Medicine Department, Xinjiang Medical University, Urumqi-830011, Xinjiang Uighur Autonomous Region，, PR China ;2Neurology Department, Xinjiang Medical University Affiliated Traditional Chinese Medicine Hospital,, Urumqi-
830000, Xinjiang Uighur Autonomous Region, PR China; *Corresponding author: Traditional Uighur Medicine Department,
Xinjiang Medical University, 393 Medical University Road, Urumqi, 830011 , Xinjiang Uighur Autonomous Region，PR China; Tel.: +86 991 4362206 fax: +86 991 436 ，E-mail: [email protected]
Karapxa decoction, is a traditional Uighur medicines formula for hepatitis, cholecystitis， gastralgia, gout, and arthralgia, anti-edema prescription in Traditional Uighur Medical literatures and State Uighur
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Medicine Pharmacopoeia of People’s Republic of China. Karapxa decoction is one of the most frequently prescriptions in Traditional Chinese Medicine to treat gout and hyperuricemia. In the present study, we investigated the water extracts of Karapxa decoction (KD) at 200，400 and 800mg/kg for 14 days were demonstrated to possess in vivo potent hypouricemic effects in both hyperuricemic mice induced by yeast extract paste and potassium oxonate respectively, and standard drug allopurinol (10mg/kg) was given as a positive control. Scavenging activity of KD on 1, 1-diphenyl-2-picrylhydrazyl radicals (DPPH), nitric oxide (NO), superoxide (O2.), efficiency against lipid peroxidation, and xanthine oxidase (XO) inhibitory effects were determined in vitro. Serum levels of uric acid, mouse liver XO activities were measured in hyperuricemic mice treated with KD and allopurinol. The results demonstrated potent hypouricemic effects both in hyperuricemic mice, KD can reduce UA and inhibit the liver XO activity. In addition, The KD extract showed noticeable antioxidant activity, through the ability to scavenge free radicals (DPPH, NO and O2.) and efficiency against lipid peroxidation, as well as has inhibitory effects on XO activities in vitro. This function could make Karapxa decoction a potentially valuable drug for the treatment of hyperuricemia and gout, and effects may be achieved by other mechanisms in part because of XO inhibition and antioxidant function.
A-177Kali Zira （Vernonia anthelmintica Willd ）injection induced allergic reaction in 6 cases R.Umar 1, J.Abduwayet 2, Tursun Wuphour 3
1 Uighur Medicine Hospital of Xinjiang Uighur Autonomous Region, 830049, Urumqi, China; 2 Uighur Medicine Hospital of
Xinjiang Uighur Autonomous Region, 830049 ,Urumqi, China; 3 Traditional Uighur Medicine Institute of Xinjiang Medical
University, 830054, Urumqi, China
The Traditional Uighur Medicine injection Kali Zira injection is composed of Vernonia anthelmintica Willd extraction. It has long been used in Traditional Uighur Medicine for treat vitiligo. According to the traditional Uighur Medicine theory KD was described to treat the diseases through balancing the Mizaj in the liver, eliminating the Abnormal Balgham Hilit (damp and cold)，excreting dampness. Kali Zira injection is recorded in State Pharmacopoeia of People’s Republic of China in the Uighur Medicine volume. Kali Zira injection injection for the treatment of vitiligo with classic prescription of Uighur Medicine. It is capable of regulating abnormal balgham, promoting blood circulation and coloring, increasing melanin cell function. This paper were reviewed the drug adverse reactions in recent years. We found 6 patients has side effect or allergic reaction.
A-178Uighur Medicine Xipayi Kui Jie’an effects Gene Expression Profiles on Model of ulcerative colitis rat colon organization
Yunusi Ki1，Z.Jingping 1，Y.Mijit 1，M.Hongchao 1，Y.Mei 1，Xu Chenbo 1, Halmurat Upur 2*1Basic Medical Institute of Xinjiang Medical University, Xinyi Road 393, Xinjiang Uighur Autonomous Region ， 830011,PR China;2Traditional Uighur Medicine Institute of Xinjiang Medical University, Xinyi Road 393, 830011, Xinjiang Uighur
Autonomous Region，PR China;*Corresponding author. Corresponding author. Halmurat.Upur email:[email protected]
Objective: Apply the of transcriptomics technologies for the detection of ulcerative colitis ( UC ) in the
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rat colon tissue of the level of structure gene expression, screening of differentially expressed genes, and their correlation analysis, to explore the pathogenesis of UC and Uighur Medicine Xipayi kuijiean (KJA) mechanisms of action.Methods: Wistar rats were randomly divided into normal group, model group, using 2,4-dinitrochlorobenzene( DNCB ) and acetic acid compound induced rat model of UC. UC rats were randomly divided into model group, normal saline (NS) treated group as negative control, KJA interfered group (large-dosage group, middle-dosage group and small-dosage group), and drug intervention for 20 days. Application of chip27K Rat GenomeArray chip detection of the level of rats colonic tissue structure gene expression, screening of differential expression gene and bioinformatics analysis. Using real-time quantitative fluorescence PCR technique on the part of differentially expressed genes were further verified. Results: The rats in the model group of symptoms, signs, colonic tissue specimens, pathological findings were consistent with UC model standard; compared with the normal group, model group have 1054 differentially expressed genes, in which the expression of 667 genes were up-regulated, 387 genes were down-regulated. Compared with normal saline negative control group, different doses of the drug intervention group in the middle of 667 up-regulated genes have 414 gene expression were down-regulated, in the expression of 387 genes down-regulated have 168 up-regulation of gene expression. Differential gene expression related with intercellular signaling, metabolism, immunity, inflammation and other life activities, including TLR2, IL-1 β, NF-κB and TGF-β1 genes using real-time quantitative fluorescence PCR technique of mRNA level of verification, the results show real time fluorescent quantitative PCR findings with microarray results have a good consistency. Conclusion: We concluded that the pathogenesis and KJA therapeutic effect of UC rats were associated with changes of gene expression profile. On differential gene expression analysis will contribute to further elucidate the mechanism of UC and KJA mechanism of action, and provide novel therapeutic targets of UC.
A-179Uighur Medicine Xipayi Kui Jie’an effects appreciation and apoptosis on HT-29 human colon cancer cells
K.Yunusi 1，Y.Mijit 1，Z.JingPing 1，L.JingJing 1, Halmurat Upur 2*1 Basic Medical Institute of Xinjiang Medical University, Xinyi Road 393, 830011, Xinjiang Uighur Autonomous Region，PR China;2Traditional Uighur Medicine Department of Xinjiang Medical University, Xinyi Road 393, 830011,PR Xinjiang Uighur
Autonomous Region，China;*Corresponding author. Corresponding author. Halmurat.Upur email:[email protected]
Objective: To investigate the Uighur Medicine Xipayi kuijiean (KJA) effects appreciation and apoptosis on HT-29 human colon cancer cells, in order to prevent and cure ulcerative colitis tissue cell carcinogenesis to provide experimental basis. Methods:Using four methyl thiazolyl tetrazolium (MTT) method to determine the proliferation inhibition rate of various concentrations KJA in human colon cancer HT-29 cell, flow cytometry technique was used for cell apoptosis. The application of different concentrations of KJA intervention in human colon cancer HT-29 cells at the time of 3h,6h,12h,24h,48h,72h, observed influence of the cell proliferation and apoptosis that KJA on HT-29 human colon cancer cell. Results: KJA intervention in human colon cancer HT-29 cells, the decrease in the number of live cells, cell proliferation was inhibited. Along with the extension of time, the same concentration of KJA in human colon carcinoma HT-29 cell inhibition rate increased, the difference was statistically significant (P<0.05); the same time, with the increase of concentration, KJA raise the cell
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proliferation inhibition function, the difference was statistically significant (P<0.05), different concentrations of group differences were statistically significant ( P< 0.05). KJA induced apoptosis in human colon cancer HT-29 cells effect was concentration, time dependent. Conclusion: KJA in human colon carcinoma HT-29 cell has certain inhibition of proliferation, apoptosis effect, so the KJA may be used as candidates drug to prevent ulcerative colitis tissue cancerous.
A-180Concept, classification and Analysis of Uighur medical toxic drugs in Traditional Uighur Medicine Hospital of Xinjiang Uighur Autonomous Region
A.Turdi1*1Traditional Uighur Medicine Hospital of Xinjiang Uighur Autonomous Region, Urumqi, Xinjiang 830001; *Corresponding
authors: Alip Turdi, E-mail: Alip [email protected]
Uighur Medicine is scientific summary and wisdom of hardworking Uighur people’s activity about fighting against with diseases over the long-term production practice. It has 2,500 years of history, a complete theoretical system, abundant practical experience and unique methods of diagnosis and treatment. So, it is a gem in the treasure-house of Chinese traditional medicine. It is mainly based on Uighur Medicine theory system to study the basic theory of Uighur Medicine, and is a series of relevant knowledge about practical experience, drug potency, Drug properties, function, main treatment, usage, dosage, note, Corrective medicine, Alternative medicine and origin, harvest, as well as the processing. It takes the most significant part of the Uighur Medicine. Xinjiang Uighur Autonomous Region covers about one- sixth of China's total territory, it is our largest and resource-rich province. Resources of Uighur Medicine are also extremely rich. Since the founding of the People's Republic of China, there have been not only more systematic understanding about the types of resources of Uighur Medicine, distribution, conservation, and ecological environment , but there have Also been discovered a new drug source by making more and more investigation and study on Uighur Medicine resources. According to preliminary statistics on Varieties of Uighur Medicine, now there is 1100 kinds’ variety: More than 1000 kinds of Botanicals, 80 kinds of Mineral drugs and 50kinds of Animal Drugs. They are mainly distributed in the north and south of the Tianshan: Oasis, grasslands, deserts, rivers and lakes of the Tarim and Junggar Basins. Tianshan Mountains and the hills and forest of the Pamir Also are all the resources of Uighur Medicine distribution. The source of Uighur Medicine was divided into three categories: Plants, animals and minerals. Botanicals can be divided into flowers, leaves, bark, roots, juice, fruit, oil, etc. Animal Drugs can be divided into hair, horn, milk, oil, dung, hoof, blood, eggs, skin, bone marrow, etc. Mineral drugs can be divided into salts, stone, metals, precious stones, oil, soil, etc. With the development of medicine, the way for classifying Uighur Medicine is improving continuously. Initially, medicine was classified to with poison and no poison; there was also a way that classifies medicine according to treatment. Deferent way of classification has its different characteristics. The concept of toxic medicine in Uighur Medicine is the medicine that has direct harm to Kuwat (three power :spirit, vitality, Natural force) or can damage as the starting point for three power of Control organ’s: (brain, heart, liver) function, and can directly bring great threat to vitality and can make body sick. The medicine which has beyond level three or four of Medicinal properties can be regarded as toxic drugs.
A-181
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Clinical effect observation on treatment Adenomyosis with Traditional Uighur Medicine
M.Abliz 1 1Traditional Uighur Medicine Hospital of Xinjiang Uighur Autonomous Region, Urumqi, Xinjiang 830049, China
Objective: To explore the effect of Traditional Uighur Medicine for treatment adenomyosis. Methods: 48 cases of adenomyosis were treated with Traditional Uighur Medicine for three months, the symptom and signs, the follow-up, B-Ultra sonic were reexamined. Results: After for three month treatment total effective of 40 cases was 87.5%, cure rate was 29.2%, significant efficiency 62.5%, After the treatment, the symptoms improved obviously and that of the treatment group was far more obvious, significantly relief, dysmenorrhea significantly decrease menstrual quantity, Endometrial thickness obvious become thin, in the cured patients uterine, volume and placed former comparison, significant changes. Conclusion: Traditional Uighur Medicine therapy for treatment adenomyos is can control condition development improve clinical symptom.
A-182Clinical report of the Mongolian psychosomatic medicine interaction therapy for the treatment of complex disease
Na Gong Bilige1，A Runa1，Chang Ming1，Bao Yin De Li Ge Er1，Chen Fu Yu1, Cao Yao Le Tu1 1Inner Mongolia International Mongolian hospital, Hohhot University of East Road No. 83, 010016, Hohhot, China
Mongolian medicine thinks that man is composed of body and spirit, and the life activities of an whole organic system runs both at its independent and unified mechanisms, in which the body is the material basis of mental activities, and the spirit is the life activities power of the body. The body is composed of three element energies and seven substances, in which the seven substances are the basic material of human body and the three element energies are the body's life-power. If the body is healthy, the three element energies maintain a balance, and imbalance will cause diseases.
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A-183On the Mongolian understanding and dialectical treatment of pigmented purpuric dermatoses
Chen Shana1
1Inner Mongolia International Mongolian Hospital Hematology, the Hohhot Saihan University of East Road No. 83, 010065,
Hohhot, China
Pigmented purpuric dermatoses is a common group disease clinically. The main characteristic of it is capillary inflammation which is caused by red cells that are exudates from leather to the outer of blood vessels and appear purpura and significant hemosiderosis. At present, there are no effects of treatment. The Mongolian medicine theory is that Pigmentad purpuric dermatoses belongs to "Xuezheng" category, mainly because of the disorder between the Stagnant blood and Huangshui function. The Mongolian medicine divides Xuezheng into two types in clinic: blood heat and blood stasis. Given pure Mongolian treatment, the total effective rate can amount to 88.5%.
A-184Persist in the Combination of Scientific Culture and Humanities Culture in the Modernization of Mongolian Medicine
Te Buqin1,2, Bai Changxi1*1Pharmacy Institute of Mongolia Medical, Inner Mongolia Medical University, 010110, Hohhot , China; 2S cientific and technical
department, Inner Mongolia Medical University, 010110, Hohhot , China ; * Correspondence to: [email protected]
The scientific culture puts emphasis on seeking the truth and forms the cultural system based on the scientific knowledge, methods and thoughts. Humanities culture mainly focuses on will, emotion, consciousness and belief and forms the cultural system based on humanity knowledge, thoughts and spirit. Traditional Mongolian medicine belongs to the traditional medicine of China, and is one of the important components of the traditional culture of China. It is built and developed in the soil of the traditional culture, and has a lot of humanistic and cultural factors. Therefore, under the new historical conditions, we should insist on the combination of the scientific culture and humanities culture to achieve modernization of Mongolian medicine.
A-185The antibacterial and diuresis action of Mongolian medicine “yunga-4”
Guqid1
1Mongolia health science and technology university doctor graduate student, International Mongolian medical hospital of Inner
Mongolia
Objective: To study the yunga-4 "Mongolian antibacterial function and diuresis action. Methods: In vitro antimicrobial experiment using AGAR dilution Mongolian medicine "yunga-4" minimum bacteriostasis of concentration (MIC) and the broth dilution minimum bactericidal concentration (MBC) were used. In
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body, antibacterial experiment used bacterial systemic infections of animal models was used to observe the yunga-4 "Mongolian medicine’s effect in mice and the metabolism of Mongolian cage observation of the yunga-4 "diuretic effect. Results: The yunga-4 "Mongolian medicine of e. coli, fu's dysentery bacillus, the common proteus, staphylococcus aureus, urinary tract infection clinical common bacteria have in vitro antimicrobial effect, Mongolian" yunga-4 "could decrease the e. coli, staphylococcus aureus infection mice mortality. Mongolian medicine "yunga-4, can increase the normal rats 3 h, 6 h, 9 h urine volume. Conclusion: The yunga-4 Mongolian medicine has antibacterial and diuresis action.
A-186The sand treatment, Mongolian medicated bath, Mongolian medicine for oral administration: three combined treatments of rheumatoid arthritis
A la Teng Chu Lu1
1Chinese-Mongolian hospital, Alashan Zuoqi, Alashan League, Inner Mongolia (750306)
Rheumatoid arthritis is a common clinical disease, especially in the countryside and pastoral areas, it has high incidence of disease. In recent years, in different reasons, rheumatoid arthritis and skin disease are increasing. It seriously affects the farmers’ and herdsmen’s health. I have been a doctor in countryside and pastoral area for many years. During this time, I have explored and studied on how to use the sand treatment and Mongolian medicated bath to treat different kinds of rheumatoid arthritis and skin disease. From this experience, I have accumulated a number of clinical experiences and had some certain achievements.
A-187The experience of cervical cancer treatment combined with Mongolian medicine European medicine
Bai. YH 1, Wang. CM 2 1 Mongolian health sciences university ,Uulanbaatar, 210644, 2 Inner Mongolia zharuud, 029100
Objective: To the observation of the impact on neutrophil and cd4/cd8 which were influenced by the cervical cancer treatment combined with Mongolian medicine and European medicine. Methods: 60 cases of cervical cancer patients were grouped presently into treatment group and compared group(n=30cases) randomly, with each group treated 3 weeks of chemotherapy containing oxaliplatin. The treatment group was treated by Mongolian medicine New ulijureel + oxaliplatin each time during chemotherapy in conjunction for a total of 21d, compared to peripheral blood neutrophils in patients with six cycles of chemotherapy and two groups of patients’ cd4/cd8 absolute value and proportion. The compared group was treated with chemotherapy drug treatment alone. We observed and compared the end of the treatments and the clinical efficacy of the two groups. Result: the neutrophils and cd4/cd8 proportions after the treatment group of using Mongolian medicine new ulijiureel+chemotherapy are
higher than chemotherapy alone group significantly (p<0.05). Conclusion： the treatment combined
with Mongolian medicine and European medicine can improve chemotherapy_related
symptoms ， reduce the chemotherapy, bone marrow suppression, regulate the immune status of
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patients treated with chemotherapy. Chemotherapy is carried out smoothly. It is worthy of further study.
A-188A Clinical Study on Changes of TCM Syndrome and Relevant Factors in Patients with Esophageal Carcinoma before and after Radiotherapy
Wu Langjie1, Du Yeqin1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China
Objective: Before and after radiotherapy, to investigate the relation of TCM syndrome differentiation, cytokines and clinical features in patients with esophageal carcinoma. Methods: The classification of TCM syndromes was carried out in 80 esophageal carcinoma patients before and after radiotherapy.
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The 80 patients' serum levels of TNF-α, IL-6 and IL-1 were measured by method in ELISA Test and Electrochemiluminescence immunoassay. Results: (1)Before radiotherapy the syndromes of qi and phlegm stagnation were 27 cases, 33.75%; phlegm and blood stasis were 34 cases, 42.5%; Yin deficiency with noxious heat were 15 cases, 18.75%; qi and yang deficiency were 4cases, 5.00%. How-ever, after radiotherapy the syndromes of qi and phlegm stagnation decreased to 5 cases, 6.25%; phlegm and blood stasis decreased to 16 cases, 20.0%; the syndromes of Yin deficiency with noxious heat increased to 45 cases 56.25%, qi and yang deficiency increased to 14 cases 17.5%. Before and after radiotherapy TCM syndrome constitutes a significant difference compared (P<0.05). (2)The levels of TNF-α were 34.34±25.28pg/ml, which were remarkably higher than the levels of TNF-α after radiotherapy 26.75±21.37pg/ml (P=0.012). The levels of IL-6 were 21.11±13.03 pg/ml, which were also remarkably higher than the levels of IL-6 after radiotherapy 17.34±13.02pg/ml (P=0.031). After radiotherapy the 2 kinds of levels in the Yin deficiency with noxious heat patients were statistically different, but the others were not. (3)The syndromes of TCM after radiotherapy were significant correlated with the levels of TNF-α. Meanwhile, the syndromes of TCM after radiotherapy were also significant correlated with Clinical features, such as esophageal cancer differentiation, KPS scores, bone marrow suppression indexing, short term effect. Conclusion: Radiotherapy makes patients with esophageal carcinoma become Yin deficiency with noxious heat. Yin deficiency with noxious heat type after radiotherapy in patients with esophageal cancer from the excessive secretion of TNF-α, IL-6 significantly curbed. After radiotherapy, changes in TCM syndromes were related with cytokines TNF-α and clinical features of esophageal cancer and degree of differentiation, KPS scores, bone marrow suppression as well as short-term effect on indexing.
A-189Analysis of Bacteria Flora in Nasal Cavity on 102 Cases Acute Asthma
Ma Huan1, Li Fengsen1*1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous
Region, China; *Correspondence should be addressed to [email protected]
Objective: This paper is to summarize the difference between acute asthma patients and average person of bacteria flora in nasal cavityon acute stage so as to find the correlation between distribution of nasal cavity bacteria flora and morbdity of asthma ,and to summarize the characteristic of nasal cavity bacteria flora on patients of asthma with different TCM syndrome type to supply the experiment basis. Methods: To separate and cultivate nasal secretions from 102 cases acute asthma patients and 42 cases average person and then to test pure colonies with bacilli biochemical assessor. Results: 1.In both of the asthma group and control group ,the detectable rate for nasal cavity bacteria flora was the highest of staphylococcus, the main bacteria flora in asthma group was single bacteria flora while the multiple bacteria flora in control group ,there were significant difference on single ,two and multiple bacteria flora in both groups(P<0.01);2.The main bacteria flora in asthma patients with different TCM syndrome type was staphylococcus , the detectable rate had no significant difference on asthma patients with different TCM syndrome type(P>0.05)， the detectable rate had no significant difference on nasal cavity bacteria flora of asthma patients with different TCM syndrome type(P>0.05)；3.The detectable rate of various bacteria flora had no significant difference on age, disease course, sex and incidence of asthma patients(P>0.05). Conclusion: 1.The patients of asthma manifested by imbalance
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of bacteria flora in nasal cavity ,and the main bacteria flora is staphylococcus which may result in reducing barrier function and infection of respiratory tract, consequently inducing asthma; or the recrudescence of asthma may destroy the bacteria flora diversity in nasal cavity thereby cause vicious circle, while the reason still require further research.2.the bacteria has no difference in patients of asthma with different TCM syndrome type because of oneness in nasal cavity.
A-190BiPAP combined with Acupuncture in the treatment of Chronic Obstructive Pulmonary Disease complicated with Pulmonary Encephalopathy research
Wei Chenggong1, Fu Ziyi1, Liu Xiaohong1, Sun Zhijia1, Dai Yong1, Liu Jianbo1
Objective: To compare Noninvasive Bi Level Positive Airway Pressure Ventilation combined with Acupuncture on Chronic Obstructive Pulmonary Disease complicated with Pulmonary Encephalopathy patients blood gas analysis, computer synchronization rate, intubation rate and other indexes, the evaluation of the treatment. Methods: 60 patients were randomly divided into treatment group and control group in respiratory department of internal medicine hospital COPD in pulmonary encephalopathy from 2010.6 -- 2012.07. The control group received routine measures combined with BiPAP treatment, the treatment group treated with Xingnao Kaiqiao acupuncture method. To observe the two cases in the ventilation before the 1h,4h and 24h, ventilation and discharged in stable condition when the results of arterial blood gas analysis and treatment course of man-machine synchronization rate, intubation rate index. Results: Of the 26 cases in the treatment group, 25 cases were successful with 1 patient required intubation and mechanical ventilation; in the control group of 27 cases 20 cases were successfully treated with 7 patients required intubation and mechanical ventilation. The rate of the treatment group was significantly higher than that of control group with machine synchronization, and the tracheal intubation rate was lower than that of the control group, which was statistically different. The successful treatment of treatment group and control group patients with blood gas analysis results show that compared with the control group the treatment of initial 4h, pH, PaCO2 in treatment group was improved significantly (P<0.05), PaO2 had no obvious difference; in the treatment of 24h and after the end of the treatment group treated with pH, PaCO2, PaO2 were obviously improved (P<0.05) than those in the control group, which was statistically different. Conclusion: BiPAP combined with Xingnao Kaiqiao acupuncture can improve the COPD with pulmonary encephalopathy with man-machine synchronization rate, reduce the intubation rate, carbon dioxide retention of patients was improved, and there were no observed adverse reactions. The study is worthy of clinical application.A-191Clinical Evaluation and Lung Function of Plastering Therapy of TCM for Chronic Obstructive Pulmonary Disease in Stable Phase in China: A Systematic Review of RCTs
Li Fengsen1*, Gao Zhen1,2, Jing Jing1, Xu Dan1
1 Affiliated Traditional Chinese Medicine Hospital，Xinjiang Medical University& National Clinical Research Base of Traditional Chinese Medicine, Urumqi, 830000, Xinjiang Uighur Autonomous Region,China; 2Xinjiang medical university, Urumqi, 830011,
Xinjiang Uighur Autonomous Region, China；*Correspondence should be addressed to [email protected]
Objective: To assess the clinical evaluation and lung function of plastering therapy of TCM for chronic
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obstructive pulmonary disease in stable phase. Methods: The Literature published before October of 2011 was searched in CNKI, VIP, and CBM to identify the randomized controlled trials (RCTs) about plastering therapy of TCM for chronic obstructive pulmonary disease in stable phase in China. The literature was screened according to the inclusive and exclusive criteria by two reviewers independently, and the methodology quality was evaluated after abstracting the data, then the RevMan 5.0 software was used for Meta-analyses. Results: Thirty RCTs involving 496 patients were included, the clinical trial groups were 1690, and the control groups were 1611. The results of Meta-analyses showed that, for clinical evaluation, compared with western medicine(WM), the plastering therapy(AT) of traditional Chinese Medicine(TCM) plus WM was significantly better［MD=4.00，95%CI（2.93，5.46），P＜0.00001 ］ . AT plus TCM were better than TCM ［MD=2.05，95%CI（0.92，4.99），P=0.089］ ,and AT plus WM plus TCM were better than WM［MD=3.19，95%CI（1.49，6.82），P=0.003］ , all the groups included in meta-analysis containing AT were better than not［ MD=3.63， 95%CI（ 2.84， 4.65）， P＜ 0.00001］ . Conclusions: the existing evidences show that plastering therapy of TCM can improve clinical efficacy of chronic obstructive pulmonary disease. However, for the poor quality of included studies, the higher quality of RCTs should be performed for better results.
A-192Comparison between the Mongolian and Modern Medical Understandings of the Regularity of Infectious Disease Course Development
Tu Men Wu Li Ji1, L. Guogua 2
1Mongolian Medicine Department, the First Affiliated Hospital of Inner Mongolia Medical University, 010050, China; 2Pneumology Department, the First Affiliated Hospital of Inner Mongolia Medical University, 010050, China
As two different medical systems, Mongolian medicine and modern medicine have their own understandings and treatments of infectious diseases. Meanwhile, because they are treating the same type of diseases, there are many similarities between them. For instance, Mongolian medicine and modern medicine almost agree on their understandings of the regularity of infectious disease course development. However, Mongolian medicine persists in dialectical treatment in curing infectious diseases, which obviously differs from modern medical emphasis on anti-pathogenic micro-organism treatment.
A-193Correlation between prothrombotic state markers and TCM syndrome type in Uygur and Han patients with premature coronary heart disease
L.Hao 1, Wang Xiaofeng1, Zhai Xueqin1, C.Jihong 1, Z.Pengcheng 1, H.Yi 1, A.Naerhan 1
1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;* Correspondence should be addressed to [email protected]
Objective: To investigate the correlation between prothrombotic state markers and TCM syndrome type
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in Uygur and Han patients with premature coronary heart disease (PCAD). Methods: 626 patients were diagnosed as CHD in Xinjiang Medical University, including PCAD 340 cases (Uygur 154 cases, Han 186 cases) and late-onset CHD 286 cases (Uygur 106 cases, Han 180 cases). Patients with traditional risk factors were recorded after hospital admission, and were conducted syndrome type differentiation of traditional Chinese medicine by attending physicians or physicians in Cardiovascular Department. The morning fasting venous blood 2ml with separation of serum were collected from patients enrolled in the second day after admission. The methods used were as follows: Fibrinogen (Fg/FIB) using automated latex enhanced turbidimetric immunoassay method, tissue plasminogen activator(t-PA), plasminogen activator inhibitor(PAI-1) using ELISA method for the determination of-two, D dimer(DD), P-selection(P-Sel) using the ELISA double antibody sandwich assay test to check. Results: For PCAD patients of Uygur and Han Nationality, the levels of PAI-1, Fg and DD had statistically significant difference compared with those in the late onset group of the same nationality. The PAI-1 level of turbid sputum group, and the levels of PAI-1, P-Sel and F g, of Qi and yin deficiency group had significant difference compared with the late onset groups of the same nationality; turbid sputum syndrome of Uygur patients with PCAD is positively related with P-Sel, t-PA, PAI-1, F g and negatively correlated with DD; turbid sputum syndrome of Han patients with PCAD is positively related to P-Sel, PAI-1and F g, and negatively correlated with t-PA; Qi deficiency and blood stasis was positively correlated with DD. Conclusion: For Uygur and Han nationality patients with PCAD, there is correlation between syndrome type of Traditional Chinese Medicine and the markers of prethrombotic state, which can serve as a guide in determining the Traditional Chinese Medicine syndrome type of PCAD.
A-194Different Syndromes in Patients with Asthma-related Changes in Immune Indexes
Su Jun1, Li Fengsen1*, Ma Huan1
1Affiliated TCM Hospital ，Xinjiang Medical University, 830000, Urumqi, Xinjiang Uighur Autonomous Region ， China；*Correspondence should be addressed to [email protected]
Objective: Through the blood of patients with different syndromes of acute asthma attack of CD4+、CD8+ T-lymphocytes, eosinophil cationic protein (ECP), total serum immunoglobulin E, (T-IgE) and its saliva, sputum, Determination of secretory immunoglobulin A (SIgA) in nasal secretions to understand and summarize the different syndromes in patients with asthma-related changes in immune parameters characteristics for Traditional Chinese Medicine for asthma in acute exacerbation of the syndrome differentiation to provide an objective basis. Methods: The collection of different syndromes in patients with asthma, 200 cases were detected by ELISA in the ECP in peripheral blood of patients with asthma, T-IgE as well as sputum, saliva, nasal secretions, SIgA; the use of flow cytometry to its blood of CD4+ and CD8+ T-lymphocytes detected; Results: (1) asthma patients in the blood of CD4+ and CD8+ T-lymphocytes, CD4+/ CD8+ expression with the normal group were significantly increased comparatively, and saliva, sputum, nasal secretions, SIgA content compared with the normal group was significantly lower, the difference was statistically significant (P <0.05); (2) different syndrome types in patients with asthma peripheral blood of CD4+, of CD8+ T-lymphocytes of CD4+/ CD8+, T-IgE, the ECP and saliva SIgA content relative to comparison no obvious difference (P0.05). (3) sputum, nasal secretions of SIgA levels compared between the different syndromes in patients with asthma, the difference was statistically significant (P <0.05), cold whooping sputum of patients with SIgA content
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higher than the wind sputum roaring, virtual heavy breathing, cold pack, hot heavy breathing patients (P <0.05), while the SIgA content in nasal secretions were lower than the wind sputum roar, cold pack hot Asthma patients (P<0.05); compared to the other groups there are no significant difference (P>0.05). Conclusions: (1) Different syndromes in patients with asthma in acute exacerbation of immune dysfunction exists a difference (2) Changes in different syndromes in patients with asthma in acute exacerbation of immune parameters, SIgA changes in respiratory secretions on which the difference is particularly evident. Therefore, the detection of patients with asthma-related immune parameters helps to provide objective and theoretical basis for the syndrome differentiation of Traditional Chinese Medicine for asthma.
A-195Explore the scientific meaning of “Lung Governing Skin and Hair” from SIgA in secretions of asthma, urticaria patients
Zhang Yanli1, Li Fengsen1*1 Affiliated Traditional Chinese Medicine Hospital，Xinjiang Medical University& National Clinical Research Base of Traditional Chinese Medicine, Ulumuqi, 830000, Xinjiang Uighur Autonomous Region, China *Correspondence should be addressed to
Objective: The common pathological embodiment and rules of mucosal immunity changes in the disease process of bronchial asthma and chronic urticaria were sought, and the relevancy between pulmonary diseases and dermatosis was discussed through determination of secretory immunoglobulin-A (SIgA) in saliva, sputum and nasal secretions, thereby further discussing the scientific connotation of ‘Lung Governing Skin and Hair ’Theory. Methods: 32 patients with pure bronchial asthma (asthma group), 33 patients with bronchial asthma combined with chronic urticaria (combined group), 31 patients with chronic urticaria (urticaria group) and 30 normal people (normal group) were collected, ELISA method was adopted to test SIgA in their sputum, saliva and nasal secretions. Results: 1）The SIgA contents in saliva and nasal secretions were higher in Asthma, Urticaria, and Combined groups, compared to Control group（P<0.01） . SIgA in nasal secretion had no significant difference among the three disease groups（P>0.05） . SIgA in saliva secretion was higher in Uriticaria, compared to Combined Group（P<0.01）2) SIgA in sputum secretion was lower in Asthma and Combined groups, compared to control and Urticaria groups（P<0.01）3)SIgA concentration in the saliva and sputum of subjects in the Control Group had a negative correlation（ r=-0.369, P =0.045）Conclusion: SIgA have common embodiment in patients with bronchial asthma and chronic urticaria, namely common expression in pulmonary diseases and dermatosis, which further embodies the ‘lung governing skin and hair’ theory, and mainly reflects in pathology， and the mucosa immune-related media may be the material basis of contact between the two.
A-196Intestinal Microflora structure and function analysis in Uighur Traditional Medicine syndrome of asthma
Li Chunyan1, Halmurat Upur2*1Basic Medical Institute，Immunology Department of Xinjiang Medical University, Urumqi 830011, China; 2Traditional Uighur
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Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; *Correspondence
should be addressed to [email protected]
Objective: Syndrome differentiation is the most important foundation of Traditional Uighur Medicine. Within the abnormal Hilit formation, the abnormal Savda is the common pathological basis and clinical syndromes in the most complex diseases. It is profound to further make clear and comprehend the biologic basis for abnormal Savda syndrome of Traditional Uighur Medicine theory. Methods: In this study, fecal bacterial diversity in abnormal Savda asthma patients (n=64) and non-abnormal Savda asthma patients (n=80) were profiled by 454 pyrosequencing of the V3 region of the 16S ribosomal RNA gene. Results: Principal component analysis showed structural segregation between the two groups. Shannon diversity analysis showed both the microbial groups and abundance were decreased in the gut microbiota of abnormal Savda asthma patients (One-way ANOVA test, P＜0.05). Twenty-three operational taxonomic units (OTUs) were indentified by redundancy analysis as key variables significantly associated with the structural differences. 5 OTUs belonging to the genera Bacteroides, two OTUs belonging to the genera Parabacteroides of the phylum Bacteroidetes, and one OTU belonging to the genera Veillonella of the phylum Firmicutes were enriched in the gut microbiota of abnormal Savda asthma patients, whereas 4 OTUs belonging to the genera Subdoligranulum, 4 OTUs belonging to the genera Faecalibacterium, and one OTUs belonging to the genera Roseburia of the phylum Firmicutes were enriched in that of non-abnormal Savda asthma patients. At the family level, one OTU belonging to the family Lachnospiraceae were more abundant in the gut microbiota of abnormal Savda asthma patients, and 4 OTUs belonging to the family Ruminococcaceae and Prevotellaceae were less abundant. Conclusion: Asthma patients with abnormal Savda syndrome had similar changes and characteristic in gut microbiota structure, which showed obvious significance differences between two groups. The results indicated the changes of key bacterial groups maybe the important factors associated with the pathological process of abnormal Savda syndrome, and provided some biologic basis for the abnormal change of Hilit of Traditional Uighur Medicine theory.
A-197Study on Metabonomics Common Characteristics of COPD about Traditional Chinese Medicine Kidney Depletion Phlegm and Blood Stasis Syndrome and Uighur Medicine Abnormal Savda Syndrome
Xu Weifang1, Halmurat Upur2*, Li Fengsen1
1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China ；2 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China, *Correspondence should be addressed to [email protected]
Objectives: This study from the metabonomics Angle, we regarded COPD, and explored COPD of metabonomics change by nuclear magnetic resonance (NMR)technology, and utillizing TCM and Uighur theory as the guide, analysed COPD in different points among the TCM types and Uighur types and the general population and the structure of the differences between the phenotype metabolism, through looking for and identifying the metabolic biological markers, we would elaborate the metabonomics aspects of the common characteristic changes and material foundation of TCM kidney empty phlegm and blood stasis syndrome and Uighur Medical Abnormal Savda syndrome, make COPD of medical
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syndrome differentiation and disease more objective and standard so as to guide clinical work for clinical diagnosis of COPD, and provide the new ideas and methods. Methods: 1) There were total of 152 cases of patients with COPD, cases source from October 2008 to March 2010 in affiliated hospital of Xinjiang Medical University, then those had been diagnosed according to western medicine diagnosis classification, and had been divided into four groups according to TCM and Uighur Medicine on syndrome differentiation and classification. Meanwhile, a total of 52 healthy volunteers composed the Normal control group, and they were from the medical center of the Affiliated Hospital of Chinese Traditional Medicine in Xinjiang Medical University. 2) All patients were classified into 4 groups according to the TCM and Uighur Medicine on syndrome differentiation and classification; 3) All patients and normal control group subjects, blood samples were tested through utiulizing the NMR metabonomics; Participants by phosphate buffer plasma treatment for 1 h NMR testing, the acquisition spectra data pretreatment, using principal component analysis (PCA), orthogonal partial least squares discriminant analysis (OPLS-DA) processing map data, data interpretation; then by Pearson correlation coefficient significant difference and two dimentions technique, metabolism composition difference were detected to the healthy people and patients of COPD with the kidney empty phlegm and blood stasis syndrome and Abnormal Savda syndrome, than the 5 groups plasma metabolites map of the differences had been researched. 4) Data processing were used mainly with Topspin 2.0 software, SIMCA-P+11 software, SPSS 13. 0 for Window. Results: 1) 1 H NMR spectrum and 2D pattern recognition in analysis showed that COPD patients with kidney empty phlegm and blood stasis syndrome group, the non-kidney empty phlegm and blood stasis syndrome group, Abnormal Savda syndrome and the non-Abnormal Savda group in comparison with the normal control group respectively, their distribution area separated completely, and COPD kidney empty phlegm and blood stasis syndrome group compared with the non-kidney phlegm and blood stasis syndrome group, and COPD with Abnormal Savda syndrome compared with the non-Abnormal Savda group，their distribution area separated also completely, many kinds of amino acids in plasma decreased significantly, including leucine, alanine, melons, acid tyrosine, l-histidine, glycine, methionine, isoleucine valine, etc, and healthy people comparing statistical significance (P＜ 0.05). In addition, glycoprotein, glutamine, inositol, lactic acid, choline, creatine also decreased significantly, the healthy and comparing statistical significance (P＜0.05). At the same time, compared with the normal control group, 1 H NMR spectrum and 2 D pattern recognition in analysis shows that, COPD patients, COPD kidney empty phlegm and blood stasis syndrome group and the non- kidney phlegm and blood stasis syndrome group, COPD with abnormal black Savda and the group with non-abnormal black Savda group completely separated in different area, metabolic component of plasma of two group have obvious difference; Many kinds of amino acids in plasma of Kidney empty phlegm and blood stasis syndrome and non-kidney empty phlegm and blood stasis syndrome group and the anomalous black Savda and the nonabnormal black Savda patients with COPD significantly reduced, metabolic components however, were basic same, many kinds of amino acids significantly reduced, the difference was statistically significant (P＜0.05); Including isoleucine, leucine, valine, alanine, glutamine, tyrosine, phen- ylalanine, the difference was statistically significant (P＜0.05). In addition, lactic acid, glycoprotein, unsaturated fat, carnitine and extremely low density lipoprotein also significantly lower than healthy people, the difference was statistically significant (P＜0.05). Meanwhile the distribution of COPD kidney empty phlegm and blood stasis syndrome group and Abnormal Savda area not separates completely, the metabolic component mentioned above of the kidney empty phlegm and blood stasis syndrome group and abnormal black Savda patients were not statistically different (P>0.05) Metabolism including isoleucine, leucine, valine,
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alanine, tyrosine, glutamine, pheny- lalanine. and lactic acid, glycoprotein VLDL and unsaturated fat also significantly reduced, carnitine was relatively higher, the difference was not statistically significant (P>0.05). extremely low density lipoprotein plasma VLDL, unsaturated lipid and acetone of COPD kidney empty phlegm and blood stasis syndrome patients and abnormal black Savda patients have shown lower, but the difference was not statistically significant (P>0.05). table 4, 12 of the difference of metabolites are expected to be serum specific biomarker of COPD kidney empty phlegm and blood stasis syndrome patients and Abnormal Savda patients. Conclusion: 1) Characteristics of COPD were close to kidney deficiency with phlegm and blood stasis syndrome of Chinese traditional medicine and Abnormal Savda syndrome of Uighur medicine, both of them were the common and typical type within the process of the development of the complexity disease with similar clinical biological characterization of serious long illness and older patients, interaction as well. 2) Metabonomics change and metabolic phenotype of COPD with kidney empty phlegm and blood stasis syndrome and Abnormal Savda are more outstanding but similar, and also with common biomarkers, mainly are threonine, leucine, valine, alanine, tyrosine, lactic acid, glycoprotein; VLDL, unsaturated lipid metabolites, etc; The result of the amino acids, fat and sugar metabolic abnormalities demostrated material metabolic disorder exist in the patients with complex diseases kidney empty phlegm and blood stasis syndrome and Abnormal Savda, and also demostrated low body's immune function, endocrine disorders and energy metabolism disorder; these methods are expected to provide new ideas and methods for early warning and forecast and clinical diagnosis d finally for big medical system complexity of the disease early warning, forecasting, treatments with new ideas and methods. 3) Metabonomics common features of Complex diseases with TCM and Uighur syndrome further reflected the interoperability three kinds of medical model of Chinese Medicine, Uighur Medicine and Modern Medicine about COPD; Make COPD of syndrome differentiation would be more objective, standard, and would be able to guide clinical diagnosis and treatment, the complexity of the syndrome differentiation and disease in guiding and outcome objective aspects to have the important meaning.
A-198Observing the clinical efficacy of combined treatment of Traditional Chinese Medicine and Western Medicine to ventilator-associated pneumonia
Zhang Yanpeng1, Lv Shuqin1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
Objective: To observe the clinical efficacy of Erchen Tang (Decoction of Pinellia and Perocarpirum) combined Maxingshigan Decoction to ventilator-associated pneumonia (VAP). Methods: 64 patients who accord with the diagnosis of VAP were randomly divided into two groups (treatment group involving 33 patient & control group involving 31 patients) .The treatment group were given conventional therapy of Western Medicine and Erchen Tang (Decoction of Pinellia and Perocarpirum) combined Maxingshigan Decoction which were taken orally 30 minutes after meals two times a day; the control group were given conventional therapy of Western Medicine. The body temperature, phlegm, bellows sign, X-ray display and white blood cell count were accumulated before the experiment and 2nd, 4th and 6th after the experiment. Result: The response rate of treatment group was 78.80%, and the rate of control group was 54.8%., which did make a statistical significance (P≤0.05). The treatment group
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made a statistical significance on improving body temperature, phlegm and white blood cell count compared with control group (P≤0.05); while it did not have the statistical significance on bellows sign and X-ray display compared with control group (P>0.05). Conclusions： There is a good clinical treatment effect of Erchen Tang (Decoction of Pinellia and Perocarpirum) combined Maxingshigan Decoction to ventilator-associated pneumonia (VAP), which can improve clinical symptoms, control infection effectively, and make patient's condition stable. In a word, it makes a positive effect to VAP.
A-199Plasma metabonomics study on the asthma diagnosed as Shenxu-tanyu syndrome in Traditional Chinese Medicine with NMR
Yan Xinghai1, Halmurat Upur2*1 Gan Su Wu Yi Central Hospital; 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: To apply the metabonomics technology about nuclear magnetic resonance (NMR), Retrieval substance metabolism way, metabolism state, discover biological marker in asthma diagnosed as Shenxu-tanyu syndrome patients by analysing and judging the interrelated specially component about plasma with asthma diagnosed as Shenxu-tanyu syndrome patients. Methods: Sodium phosphate buffer was employed to prepare plasma samples which obtained from 39 cases of Shenxu-tanyu syndrome patients with asthma and 40 healthy volunteers. A 1H NMR based metabonomic analysis was performed on these plasma samples. A multi-steps date preprocessing procedure was completed and then Orthogonal Partial Least-Squares Discriminant Analysis (OPLS-DA) was fulfilled to distinguish the metabolic phenotypes, the influential biomarkers accounting for the metabolic signature perturbation were discovered by Pearson’s product moment correlation coefficient and 2D NMR. Results: It was found that the metabolic phenotypes in Shenxu-tanyu syndrome with asthma group underwent significant variation , which resulted in the obvious classification between the health and patients groups in the score scattering plot of OPLS-DA model; the results show that in plasma of Shenxu-tanyu syndrome patients with asthma, the expression of isoleucine, leucine, valine, lactate, alanine, glycoprotein, citrate, creatine , creatinine, 1-methy histidine, scyllo-inositol see a significant decrease compared with healthy group (P<0.05), whereas , the expression of acetone, acetoacetate, carnitine significant elevated (P<0.05). Conclusion: Compared with asthma diagnosed as Shenxu-tanyu syndrome patients, there were significant differences in metablolite and metabolism net in healthy, possible by interaction between geneticfacter and environmental facter. The marker metablolite of asthma diagnosed as Shenxu-tanyu syndrome have osculate correlation with pathologic physiology change, such as airway inflammation ,airway hypersensitivity, airway remodeling. Meanwhile, during asthmatic attack period, the process of energy metabolism have significant changed in the asthma diagnosed as Shenxu-tanyu syndrome patients, particular expression in increasing metabolism fatty acid and branched-chain amino acid, restraining tricarboxylic acid cycle, strengthening glyconeogenesis, reducing compound and metabolism creatine, which predicted that asthma diagnosed as Shenxu-tanyu syndrome patient have continued analosis and immunologic function disorder in long time. The continuance and protraction stimulation in airway inflammation are the main reasons to lead to this condition. The study provided the preliminary foundation in recognizing further microcosmic physicala foundation of asthma diagnosed as Shenxu-tanyu syndrome and evaluating the intervene
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effect of Traditional Chinese Medicine to asthma diagnosed as Shenxu-tanyu syndrome.
A-200Serum Metabonomics Research on Shenxu-tanyu Syndrome Type 2 Diabetes Mellitus Based on NMR
You Li1, Halmurat Upur2*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Xinyi Road393, 830011, Urumqi , Xinjiang Uighur
Autonomous Region, China；* Correspondence should be addressed to [email protected]
Objective: To explore the metabolic variation of type 2 diabetes mellitus of shenxu-tanyu syndrome in Traditional Chinese Medicine by using nuclear magnetic resonance(NMR), analysing the pathogenesis of discrepant metabolites, finding characteristic biomarkers. Methods: Serum samples from 33 cases of shenxu-tanyu syndrome type 2 diabetes mellitus patients and 50 cases of healthy persons were detected with 1H-NMR and 2D H-NMR. The data were compiled, integrated and normalized, then identify the discrepant metabolites by Orthogonal Partial Least-Squares Discriminant Analysis (OPLS-DA). Results: It shows that it is separated obviously between the healthy people and patients groups in the score scattering plot of OPLS-DA model, compared to healthy people, there are various kinds of amino acids that are lower markedly, include isoleucine, leucine, valine, alanine, glutamate, histidine, tyrosine, phenylalanine, 1-methylhistidine, difference was statistically significant(P<0.05). In addition, lactate, citrate, creatine and creatinine are also lower markedly(P<0.05). But the α-glucose, β-glucose and very low density lipoprotein are increased compared to healthy people(P<0.05). Conclusion: (1) There are 16 kinds of discrepant metabolites which may be the potential biomarkers of type 2 diabetics of shenxu-tanyu syndrome. (2) The serum metabonomics change mechanisms of type 2 diabetics of shenxu-tanyu syndrome are closely related with glucose, lipid and amino acid metabolism disorder. (3) Type 2 diabetics of shenxu-tanyu syndrome’s tricarboxylic acid cycle and glycolysis is inhibited, protein is metabolic disorder, branched chain amino acid is metabolic boost. (4) The study on the same disease with different syndrome of type 2 diabetics is in progress.
A-201Studies on Traditional Chinese Medicines
Brantner Adelheid 1*, Lower-Nedza Agnieszka 1, Shi Yujing 2
1Institute of Pharmaceutical Sciences, Department of Pharmacognosy, University of Graz, Universitaetsplatz 4/I, A-8010 Graz,
Austria; 2China Academy of Chinese Medical Sciences, Institute of Chinese Materia Medica, No. 16 Nanxiaojie, Dongzhimen
Nei Ave, Beijing 100700, China; *Corresponding author: Prof Dr Adelheid Brantner Institute of Pharmaceutical Sciences,
Department of Pharmacognosy University of Graz Universitaetsplatz 4/I A-8010 Graz, Austria [email protected];
Phone: 0043 316 380 5528; Fax: 0043 316 380 9860
Traditional Chinese medicines (TCMs) are complex mixtures containing up to hundreds different constituents but only a few compounds are responsible for their pharmacological activities. These large numbers of constituents in the TCMs make the screening and analysis of the bioactive components extremely difficult. Chromatography is one of the main techniques applied because of its powerful
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separation and sensitive detection. WHO introduced the use of chromatographic methods for the standardization of medicinal plants and herbal medicinal products. Nowadays the high performance liquid chromatography (HPLC) together with thin layer chromatography (TLC) are the most common analytical methods to determine either the quality or the quantity of compounds in the phytochemical evaluation. The aim of this study was to optimize HPLC and TLC methods for the simultaneous quantification of the main compounds present in the Traditional Chinese formulas Huanglianjiedutang (HLJDT) and Si Miao with two modifications. Representative chromatographic fingerprinting of batches of the herbal components of the formulations originated from different Chinese provinces were applied to offer integral characterization of the single herbs. Validation of the HPLC methods was performed to demonstrate its reproducibility, precision, stability, repeatability, and recovery rate. Permeability is a major factor for orally applied drugs. Therefore permeability assay and parallel artificial membrane permeability analysis (PAMPA) were used to determine passive, transcellular drug permeability. The permeability of well known TCM formulations (decoctions of Sì Miào S n and its modifications, Yin Qiaoǎ San/decoction, Yin Qiao San/ethanol extract) was evaluated in the experiment. Hexadecane/hexane and lecithin/dodecane were used to create artificial membranes. Different parameters of the methods were evaluated.
A-202Study of acupoint application therapy on rat prothrombotic state and establishment of the corresponding animal model
Zhang Wenhui1, Song Xiaoping1
1Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
Objective: To study the influence of acupoint application therapy on rat prothrombotic state. Methods: Forty-eight SD rats were randomly divided into a model group, an acupoint application group I, group II, and an acupoint application group III. Continuous intervention of two weeks for three groups except the model one, the pre-thrombosis model was induced by Adrenalin Hydrochloride and ice water method for four groups separately. Molecular markers in arterial blood were determined 18 hours later. Results: Fibrinogen (Fbg), Von Willebrand factor (vWF), and type-1 plasminogen activator inhibitor (PAI-1) decreased after point application. The levels of Fbg, vWF, and PAI-1 in the acupoint application group I and group II were lower than those in the model group (P<0.01). The levels in the acupoint application group III was lower than those in the model group (P<0.05). And there was no significant difference in the acupoint application group I, group II, and III (P>0.05). Conclusion: The acupoint application can significantly affect contents of Fbg, vWF, and PAI-1.
A-203Study on Cytokine Regulation Mechanism of Downregulating Eosinophils By Tonifying Shen in Traditional Chinese Medicine to Prevent Asthma Relapse
Zhang Wenbin 1, Mao Bing 1*1Huaxi hospital of Sichuan university of combination of TCM and western medicine department, 610041, Chengdu, China
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Objective: In the way of methods of treatment replaced by prescription, using YGW, a typical Tonigying kidney prescription, as intervention, to observe its effects on levels of interleukin-5 (IL-5), eotaxin in peripheral blood (PB) of bronchial asthma mouse model, bronchoalveolar lavage fluid (BALF) and bone marrow (BM), as well as counts of white blood cell in PB, eosinophil (EOS), nucleated cells and EOS in BM; investigate the impact of kidney-tonifying therapy on EOS, revealing its mechanism of preventing asthma recurrence, in order to provide a theory evidence for the implication of asthma prevention by Tonifying Shen in Traditional Chinese Medicine. Method: Twenty-four SPF BALB/c mice were divided into four groups (6 in each group), which were physiological saline group (as control, A), asthma model group (B), prednisone treated group (C) and YGW treated group (D), based on the table of random number. Mice in group A underwent intraperitoneal injection of physiological saline and nebulization. For the other three groups, the mice received intraperitoneal injection of Ovalbumin (OVA) 20ug mixed with abuminum hydroxide 50ug twice to sensitize and 1%OVA by nebulised medication to stimulate. All the mice got stimulation for 1 week. After secondary sensitization, drugs (YGW capsule/prednisone) or placebo (physiological saline) were administrated for 2 weeks. After the last time stimulation, all the mice were killed within 24h. Using ELISA, the levels of interleukin-5 (IL-5), eotaxin in peripheral blood (PB) of bronchial asthma mouse model, bronchoalveolar lavage fluid (BALF) and bone marrow (BM), were measured, respectively. Meanwhile, white blood cell in PB, nucleated cells and EOS in BM were counted. Lung tissues of mice were evaluated pathologically by HE staining. Result: 1. EOS count: The count in PB was significantly increased (p<0.05) in asthma group and YGW group compared to control; asthma group got significantly increased EOS count than prednisone and YGW group did (p<0.05); however there was no significant difference between prednisone group and YGW group (p>0.05). For the EOS count in BM, it was the same as that in PB.2. White blood cell count and nucleated cell count: For the white blood cell count in PB, there was no significant influence among each group (p>0.05), the same for nucleated cell count in BM (p>0.05).3. IL-5 level: The level in BALF was significantly increased in each experimental group compared to control (p<0.05); asthma group got significantly increased IL-5 level than prednisone and YGW group did (p<0.05); however there was no significant difference between prednisone group and YGW group (p>0.05). In PB, the IL-5 level got varying increase in prednisone group and YGW group compared to control (p<0.05); but varying decrease when compared to asthma group (p<0.05); there was no significant difference between prednisone group and YGW group. The level in BM was no significant difference among control, prednisone group and YGW group (P>0.05); however, the asthma group got highest IL-5 level and it was significantly different when compared to control and prednisone group (p<0.05). 4. Eotaxin level: In BALF, there was no significant difference among control, prednisone group and YGW group (p>0.05); Eotaxin level was highest in asthma group and it was significantly different compared to control (p<0.05); compared to asthma group, prednisone group and YGW group got varying decrease (p<0.05). In PB, there was significantly increased in asthma group and YGW group compared to control (p<0.05); asthma group got significant increase than prednisone (p<0.05); there was no significant difference prednisone group compared to YGW group and control (p>0.05). In BM, there was no significant difference among control, prednisone group and YGW group (p>0.05); asthma group got highest level and it was significant different compared to control and prednisone group (p<0.05).5. Pathological section: For the mice in asthma group, they got hyperemia and edema in alveolar septum, widening septum, vessel hyeremia in pulmonary interstitium, infiltration of EOS, monocytes and lymphocytes; however, the mice in YGW group and prednisone group got narrowing alveolar septum, less vessel hyeremia in interstitium, and less infiltration of EOS, compared to asthma group. Conclusion: 1. YGW could down-regulate the level
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of IL-5 and Eotaxin in BALF, PB and BM of mice in asthma model.2. YGW reduced EOS level in PB and BM of mice in asthma model.3. YGW had alleviation on the pathological changes of lung tissues of mice in asthma mode.4. The mechanism of asthma prevention by Tonifying Shen in Traditional Chinese Medicine could be through regulation of IL-5 and Eotaxin and down-regulation of EOS level in PB and BM.
A-204Study on Esophageal Carcinoma Uygur Traditional Medicine Syndromes and the Change of T-lymphocyte Subsets and NK cells of Patients Before and After Radiotherapy
Zhou Jie 1, Du Yeqin 1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China
Objective: To investigate the esophageal carcinoma Uygur Traditional Medicine syndromes and the change of T cell subsets and NK cell level before and after radiation therapy, and the observation of radiotherapy after those medicine typing in patients with esophageal cancer with radioactive esophagitis, bone marrow suppression and near future curative effect. Method: To apply Uygur Medicine abnormal humoral classification standard before and after radiation treatment in 120 cases of esophageal carcinoma patients with Uygur Medicine typing, and before and after radiation therapy using flow cytometry in patients with peripheral blood T cell subsets and NK cell level, simultaneously recorded during radiotherapy related clinical features of toxic reactions and curative effect in the near future. Results: (1) before and after radiotherapy, the distribution of esophageal cancer patients can be drawn: abnormal savda(50%)>abnormal belghem(27.5%)>abnormal kan1(15%)>abnormal sapra(7.5%);after radiotherapy: abnormal savda(76.67%)>abnormal sapra(12.5%)>abnormal kan(7.5%)>abnormal belghem(3.33%)；The proportion of Uygur Traditional Medicine Syndromes has significant difference(P<0.01); (2) before and after radiotherapy in 120 cases of patients with esophageal carcinoma T cell subsets and NK cell level changes : CD3+、CD4+、CD4+\CD8+and NK cell levels were decreased, CD8+ before radiotherapy levels are elevated before radiotherapy, and radiation changes before and after treatment was statistically significant(P<0.05); (3) after radiotherapy, different Uygur Traditional Medicine syndromes, abnormal savda and abnormal Savda before and after radiotherapy of T cell subsets and NK cell level changes: abnormal savda patients after radiotherapy CD3+、CD4+、CD4+\CD8+and NK cell levels and before radiotherapy ratio decreased, while CD8+ level than before radiotherapy L High, and before and after radiotherapy changes were statistically significant(P<0.05); instead of abnormal Savda in patients before radiotherapy compared with CD3+、CD4+、CD4+\CD8+ 、CD8+ and the ratio of NK cell levels and before radiotherapy changes little, and the changes were not statistically significant, (P<0.05); (4) after radiation for abnormal savda of abnormal Savda in patients with esophageal cancer radiotherapy in toxicity and efficacy were compared, found between the two differences, and the differences were statistically significant (P<0.05). Conclusion: Abnormal Savda is the major Uygur Traditional Medicine Syndromes before and after undergoing radiotherapy; radiotherapy makes abnormal savda humoral form; after radiotherapy abnormal Savda patients with impaired immune function is more and more serious, and the radioactive esophagitis, bone marrow suppression and the recent effect correlation.
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A-205Study on immune and endocrine disturbance of bronchial asthma by Traditional Chinese Medicine, Uygur Medicine and Western Medicine
Li Fengsen 1, Halmurat Upur2*1Department of Respiratory Department ， Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University；2Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
Objective: In this study, we studied the relationship between asthma and immunity, endocrinium, and changing regulation in traditional Chinese medicine, Uygur Medicine and Western medicine. Discovered the rule of immune and endocrine system disturbance in patients with asthma (especially the severe asthma), explained pathogenesis of asthma, summarized the regulation between the changes of immune and endocrine systems in deficiency asthma, abnormal sewda asthma; searched the biological expression in the classification by Traditional Chinese Medicine and Uygur Medicine and relative indices of microcosmic syndrome differentiation, discriminate the concordance and reciprocation relationship among severe asthma, deficiency asthma and abnormal sewda asthma, and provide rationale basis for the diagnosis, prediction and treatment of asthma. Method: During the period of January 2006 to January 2007, 156 cases of bronchial asthma patients were collected. All cases were analyzed by severity classification, differentiation of symptoms and signs for classification of syndrome by traditional Chinese medicine and Uighur medicine. PEF% Pred, FEV1% Pred and FEV1% were detected in asthma patients by Pulmonary function testing machine (JAEGER PET) made in Germany. IL-1, IL-6, TNF-α, ACTH, CS and GH were detected with radio immunoassay while CRH with ELISA. Lymphocyte apoptosis, CD3CD4CD8, CD11b, CD18 and CD62P were determined with flow cytometry. Measurement data were described with mean and standard deviation, and multiple mean comparisons were dealt with single factor analysis of variance or group t test. Discriminant function was calculated with Fisher method. The size of test was α=0.05. Results: The course was long in patients with severe asthma, and compared with the patients with asthma of other types, the mean age of severe asthma was older. Compared with other types of asthma classified by Traditional Chinese Medicine classification, the mean age of patients with deficiency asthma was older. Compared with the other types of classified by Uygur Medicine classification, the mean age of patients with abnormal sewda asthma was obviously older. There was significant statistical difference（P＜0.05） . Lymphocyte subgroup and its mediatior of CD4, CD8, CD4/CD8, CD11b, CD11b /CD18, CD62P and TNF-αheightened gradually in accordance with the exacerbation of the disease. Those were the highest in severe asthma, while lymphocyte apoptosis decreased along with the aggravation of the disease. The apoptosis was not obvious in the severe asthma. CD4/CD8, CD11b, CD11b/CD18 and CD62P were the highest and lymphocyte apoptosis the slowest in the group of deficiency asthma. CD11b increased and lymphocyte apoptosis decreased obviously in the abnormal sewda asthma group. CS, ACTH and CRH decreased gradually in accordance with the exacerbation of the disease, and were the lowest in the severe asthma. CS, ACTH and CRH were the lowest in the deficiency asthma with differentiation of symptoms and signs for classification of syndrome by Traditional Chinese Medicine and in the abnormal sewda asthma with differentiation of symptoms and signs for classification of syndrome by Uygur Medicine. The lymphocyte subgroup and endogenous cortisol were usually affected by medicine.
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Among them, CD4/CD8, CD11b, CD11b /CD18 and CD62P heightened gradually and lymphocyte apoptosis slowed down along with the application of hormone. CS, ACTH and CRH decreased gradually according to the application of hormone. The research result that, all types had the insidous phlegm syndromes are as follows: stethocatharsis, sticky and greasy tongue coating, such as white and greasy fur, thick fur, yellowish and greasy tongue fur, smooth pulse, wiry pulse, deep pulse and so on. Besides the general symptoms of asthma, patients with deficiency asthma followed with renal deficiency syndrome such as mental exhaustion, cold body and limbs or sultriness, lassitude in loin and legs, tinnitus and loss of memory, clear abundant urine, increased nycturia and so on, stagnation of phlegm syndrome such as feeling of stuffiness in the chest, heaviness in head and body, numbness of the limbs, hydroadipsia, abundant expectoration and difficult to hawk and so on, stasis syndrome such as chest gas pains or twinge, scaly skin, pale purple tongue or dark purple tongue with ecchymosis and so on. There was reciprocity of the three types in the severity. It was shown in the severity of asthma, mean age, symptoms and syndromes, immune and endocrine system disturbance, and so on. Discrimination of patients with severe asthma, deficiency asthma and abnormal sewda asthma was carried out with Fisher method. The veracity rate was 84.1% of severe asthma, 74.1% of deficiency asthma and 66.7% of abnormal sewda asthma. Conclusion: 1) It was found that the immunity and endocrine of patients with asthma are indiscriminate. In severe asthma, lymphocyte subgroup and the value of its mediators such as CD4, CD8, CD4/CD8, CD62P,CD11b, CD11b/CD18 and TNF increases obviously. That shows negative correlation with pulmonary function. CS, ACTH and CRH decrease obviously, but the lymphocyte apoptosis is not evidently. Therefore, in combination with the previous study, besides clinical manifestation and pulmonary function, lymphocyte subgroup and its mediators, lymphocyte apoptosis, endogenous cortisol and change of IgE and ECP should be monitored dynamically when severe asthma is judged. 2) It was found that the effect of hormone therapy on immunity and endocrine is obvious. Long use of hormone will result in side-effect that it is disadvantage for the treatment on asthma. The material basis might be the gradually increase of CD4/CD8, CD11b, CD11b /CD18 and CD62P, slow lymphocyte apoptosis, and gradual decrease of CS, ACTH and CRH as well. 3) The change of CD11b and CD11b/CD18 as the relative index of infection was obvious in the onset of asthma (especially the severe asthma). It indicates that infection closely relate to the onset of asthma. 4) There is syndrome of “insidous phlegm” in all differentiations of symptoms and signs for classification of syndrome of asthma. No matter asthma belongs to sthenia syndrome, deficiency syndrome or combination of sthenia syndrome and deficiency syndrome, “phlegm” exists from the beginning to the end of asthma. “Phlegm” plays important role in the persistence and aggravation of asthma. “No phlegm could induce asthma”. “Phlegm is the key to asthma”. Therefore, we suggest that the classification of onset period of asthma, such as cold asthma, heat asthma, frigiopyretic asthma and anemophlegmatic asthma, changes to be the classification of cold phlegm, heat phlegm, frigiopyretic phlegm and anemophlegmatic phlegm. 5) In patients with deficiency asthma, there is syndrome of kidney deficiency and stagnation of phlegm. So we propose that deficiency asthma is really asthma of phlegm stagnancy because of deficiency of kidney. It needs further research. 6) We discovered that there is certain reciprocity among severe asthma, deficiency asthma and abnormal sewda asthma, which is shown by the severe condition, old mean age, overlapping of syndrome and symptom, indiscriminate immunity and endocrine, and so on. The study explained the relationship among immune and endocrine system disturbance, severe asthma classified by western medicine, deficiency asthma classified by traditional Chinese medicine, abnormal sewda asthma classified by Uighur medicine. The research is undertaking. On basis of that, we suggest that, in the treatment on asthma, we should not
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only think highly of the diagnosis of asthma by western medicine and the evaluation on patients’ condition, but also combine with differentiation of symptoms and signs and differential diagnosis of disease, and put the entire conception of traditional medicine, the thought of “man, as a microcosm, is connected with the macrocosm” into the whole process of diagnosis, therapy, prognosis judgment of asthma to improve the diagnosis and treatment on asthma.
A-206Study on Neuro-Endocrine-Immune disturbance of Type-2 diabetes Millitus by traditional Uygur Medicine and Western Medicine
Mutallip Obulkasim1, Halmurat Upur 2*1 First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region ， China; 2Traditional Uygur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to [email protected]
Objective: To investigate the relationship between neuro-endocrine-immune (NEI) systems and type-2 diabetes millites (T2DM). To explore the changing mechanisms of NEI system in different fundus changing stages in type-2 diabetes millitesy. To further explore the changing mechanisms of NEI system in capillary complication in T2DM. To explore the changing mechanisms of NEI system of T2DM in different abnormal body fluid syndromes, from the point of traditional Uygur Medicine (TUM). At the end, integrate modern Western Medicine and TUM on the T-2DM, with their constituent ratio, ages, courses and NEI systems changing characters to find out the communication of the pathogenesis, prevention and treatment of T-2DM existed in the NEI network between the two different styled medicines. Methods: Certainly clinical diagnosed 174 patients with T-2DM, were enrolled into the study from September, 2007 to February 2008. Strict and impartial screened all above the T-2DM patients by diagnostic standard and exclude standard of diabetes with ADA criteria-2005 for the diagnosis of diabetes mellitus. And 33 normal volunteer people were served as control group after physical exam proved medical fitness. Flow cytometer technology was utilized to detect the serum level count of CD3+, CD4+and CD8+, and NK cell; Radio-immune technology was utilized to detect the serum level of cytokines including IL-1β (Interleukin-1β), IL-6 ､ TNF-α (Tumor necrosis factor-α), and hormones including ACTH (adrenocorticotrophic hormone) and CORT (cortisol) ;Liquid Chromatography was utilized to detect the plazma level of DA (dopamine) and NE (norepinephrine), on every patient group and control group. TOPCON IMAGEnet 2000TM system was utilized to detect the flouressain fundus angiography of patients with T2DM. “Diagnostic method of traditional Uygur Medicine” was utilized to identify different abnormal hilit types of patients with T2DM. Results: NEI network related indexes shows that, a significant decrease of serum CD4+, CD8+ lymphocytes and NK cells count and CD4+/CD8+ ratio increased was found in T-2DM groups compared to the control group (P＜0.01); the serum level of IL-1β､IL-6 and TNF-α increased in T-2DM compared to the control group (P＜0.01); It was discovered that the serum level of ACTH and CORT decreased significantly in T-2DM compared to the control group (P＜0.01); it was discovered that the plasma level of DA and NE increased in T-2DM group compared to the control group (P＜ 0.01). By diagnostic standard and exclude standard of diabetes with ADA criteria-2005, and detecting by flourescein fundus angiography, 174 T2DM patients divided into three groups: non-diabetic retinopathy group (contains 98 patients), non-proliferating retinopathy group (56 patients), and proliferating retinopathy group (20 patients). Mean age of non-
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diabetic retinopathy group significantly smaller than non-proliferating retinopathy group and proliferating retinopathy group (P＜0.05), mean course of non-diabetic retinopathy group significantly shorter than non-proliferating retinopathy group and proliferating retinopathy group (P＜0.01), but both of the above index have no difference between non-proliferating retinopathy group and proliferating retinopathy group (P＞0.05). Comparing between different fundus groups, serum CD4+lymphocyte count in proliferating retinopathy group smaller than non-diabetic retinopathy group (P＜0.05); the serum level of ACTH and CORT decreased significantly in proliferating retinopathy group smaller than non-diabetic retinopathy group (P＜ 0.05). By diagnostic standard of “Diagnostic method of traditional Uygur Medicine”, 174 T2DM patients divided into four groups: abnormal savda group 79 patients (45.40%), abnormal khan group 39pateints (22.41%), abnormal belghem group 35 patients (20.12%), abnormal sepra group 21 patients (12.07%). Comparing between Uygur Medicine different abnormal hilit groups, a significant decrease of serum CD4+cells count was found in abnormal khan group than abnormal savda group (P＜0.01), a significant decrease of serum NK cells count was found in abnormal savda than abnormal khan and abnormal belghem group (P＜0.01); the serum level of IL-1β increased in abnormal savda group than other three groups (P＜ 0.05). Comparing between abnormal savda and non-abnormal savda groups, a significant decrease of serum CD4+and NK cells count was found in abnormal savda group than non-abnormal savda group (P＜ 0.01), the serum level of IL-1β and IL-6 increased in abnormal savda group than non-abnormal savda group (P＜0.01), the plasma level of NE increased in abnormal savda group than non-abnormal savda group (P＜0.05). The mean age is high, and mean course is long in all of the proliferating retinopathy group and abnormal savda group type-2 diabetes millets. On diagnosing, 40% of proliferating retinopathy group belongs to abnormal savda diabetes. Between the proliferating retinopathy group and abnormal savda group type-2 diabetes millets, lymphocytes, cytokines, hormones and mono-peptides have the same changing outputs. Conclusion: The nerval-endocrino-immuno network is imbalanced in the patients with type-2 diabetes milletus. The cellular immunity be weakened, humoral immunity strengthent;endocrine function weakened and the sympathic nerve system activated in type-2 diabetes milletus NEI nerwork. Among the different fundus group of T2DM, the proliferating retinopathy group has the highest mean age and longest course, and has the heaviest retinal capillary complication. So, the age and course may be the risk factor for T2DM in retinal capillary complication. It is may be the characteristic signal of type-2 diabetes milletus, that cellular immunity weakened and endocrine secretion decreasing, would lead to capillary endothelium proliferating and accelerate new vasculirezation on retina. Abnormal savda syndrome is the mean syndrome in type-2 diabetes milletes, according to the constituent ratio between the different abnormal hilit group in Uighur medicine. Comparing with other Uygur abnormal hilit group type-2 diabetes, abnormal savda group has the most imbalanced immune system (cellular immune depressed, and cytokines activated), and its sympathetic nerve activated mostly. Both of proliferating retinopathy T2DM and abnormal savda T2DM, may be the same heavier stage of T2DM, so mean age high, mean course long and pathogenetic condition severe almost in the same degree; and their immune system ambalanced, endocrine function depressed and sympathetic nerve activated in same condition. We conclude that, when there appears abnormal savda syndrome in type-2 diabetes milletus, and accompany with depressed cellular immune function and decreasing endocrine secretion, we could decide that there would be appear diabetic microvascular complication, and decide to “treat before disease”.
A-207
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Study on the Phlegm-Stasis Syndrome in Complex Diseases and the Prethrombotic State
S.Abdurhim 1, Halmurat Upur2* 1 First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region ， China; 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
Objective: To study the proportion of phlegm-stasis syndrome in complex diseases and to explore its relations to Prethrombotic State. Methods: The expression of CD41, CD62P on platelets, the level of plasma tissue plasminogen activator (t-PA), Plasminogen Activator Inhibitor-1（ PAI-1） ,Activated partial thromboplastin time (APTT), Fibrinogen ( Fib), Prothrombin time ( PT) and Thrombin time (TT) were tested by using Flow Cytometer, ELASA method and auto coagulometer. Results: 1) The proportion of phlegm-stasis syndrome in complex disease was 76.84%, non-phlegm-stasis syndrome was 23.16%; the proportion of phlegm syndrome in phlegm-stasis syndrome was 24.66%, stasis syndrome was 19.18%, stagnation of phlegm and stasis syndrome was 56.16%. 2) ① Compared to control group, there was no significant difference on the expression of platelet CD41 and TT in phlegm-stasis syndrome group(phlegm syndrome, stasis syndrome, stagnation of phlegm and stasis syndrome groups )(P>0.05); however the expression of platelet CD62P was higher (P<0.01) in phlegm-stasis syndrome group(phlegm syndrome, stasis syndrome, stagnation of phlegm and stasis syndrome groups); the level of plasma t-PA was lower(P<0.05) in phlegm-stasis syndrome group (phlegm syndrome group P>0.05, stasis syndrome, stagnation of phlegm and stasis syndrome groups P<0.05); the level of plasma PAI-1 was higher(P<0.05) in phlegm-stasis syndrome group (phlegm syndrome, stasis syndrome groups P<0.05, stagnation of phlegm and stasis syndrome group P<0.01); the level of plasma Fib was higher(P<0.01) in phlegm-stasis syndrome group (phlegm syndrome, stasis syndrome, stagnation of phlegm and stasis syndrome groups P<0.01); PT, APTT was shortened(P<0.01) in phlegm-stasis syndrome group (phlegm syndrome, stasis syndrome, stagnation of phlegm and stasis syndrome groups P<0.01). ② Compared to non-phlegm-stasis syndrome group, the expression of platelet CD62P was higher(P<0.01)( phlegm syndrome group P>0.05, stasis syndrome group P<0.05, stagnation of phlegm and stasis syndrome group P<0.01), and the level of plasma Fib of phlegm-stasis syndrome group were higher(P<0.01)( phlegm syndrome group P>0.05, stasis syndrome group P<0.05, stagnation of phlegm and stasis syndrome group P<0.01), although the expression of platelet CD41, the level of plasma t-PA, PAI-1, PT, TT and APTT were insignificantly different between two syndrome(P>0.05), each the items above had a certain gradient on phlegm syndrome, stasis syndrome, stagnation of phlegm and stasis syndrome and non-phlegm-stasis syndrome groups, and the changes were the most obvious in stagnation of phlegm and stasis syndrome groups. Conclusion: 1) phlegm-stasis syndrome is more common and is one of the important syndrome types of complex disease; stagnation of phlegm and stasis syndrome are more prevalent than phlegm and stasis, stagnation of phlegm and stasis are the common result of phlegm and stasis. 2) There is a different degree of prothrombotic state in phlegm-stasis syndrome of complex disease; the changes of prethrombotic state including the expression of platelet CD62P, the level of plasma t-PA, PAI-1, Fib, PT and APTT are more obvious in phlegm-stasis syndrome than that of the non-phlegm-stasis syndrome.
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A-208Study on relationship between TCM syndrome differentiation and Neuroendocrine-Immune-Netwok in Type-2 diabetes
Palidan Wushuer, Halmurat Upur 2*1 Urumqi City Traditional Chinese Medicine Hospital, 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi
830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: To explore the relationship between TCM syndrome differentiation and neuroendocrine-immune-netwok in Type 2 Diabetes. Methods: Collect the Uighur and Han patients of type 2 diabetes from Traditional Chinese Medicine Hospital in Urumqi Xinjiang from November 2007 to January 2008 of 105 cases, divided them into different groups according to TCM syndrome differentiation and analysis the relevant indicators. In the normal control group there are 30 cases without lung, liver, kidney and blood diseases, without autoimmune diseases and thyroid dysfunction. The Serum auxiliary/suppressor T cell ratio (CD3+ CD4+/CD3+ CD8+) and natural killer (NK) cells, Serum interleukin-1β (IL-1β), IL-6, tumor necrosis factor(TNF-α), and cortisol (CORT), adrenocorticotropic hormone (ACTH), plasma dopamine (DA) and Norepinephrine (NE) were tested by using Flow Cytometer, radioimmunoassay method and liquid chromatography. Results: Among the total clinical cases, the ratio of tan zhuo zhong zu patients was 43.7%， the ratio of yin xu re sheng patients was 20.0%，the ratio of qi yin liang xu patients was 19.3%, the ratio of xu yu mai luo and yin yang liang xu xue yu shui ting patients was 17.0%. Compared to control group，the level of CD4、CD8、NK、 ACTH、CORT of type 2 diabetes were decreased (P<0.01); The level of IL-1β、 IL-6 and TNF-αwere increased (P<0.01); the level of NE、DA were increased (P<0.05); there were no significant changes in CD4/CD8 between two groups (P>0.05). The level of DA、NE were increased (P<0.01) in TCM syndrome differentiation, the gradient changes of DA、NE was xu yu mai luo and yin yang liang xu xue yu shui ting＞tan zhuo zhong zu＞qi yin liang xu＞yin xu re sheng；the level of CORT was decreased (P＜0.05), the gradient change of CORT was: qi yin liang xu＞tan zhuo zhong zu＞xu yu mai luo and yin yang liang xu xue yu shui ting＞ yin xu re sheng. Conclusion: 1) There was a disordered status on Nerve - endocrine - immune network in type 2 diabetes. 2) The level of CORT decreased might be one of the material basis of xu zheng and zheng qi bu zu, The level of DA、NE increased might be related to shi zheng and tan yu jiao zu. 3) We propose to take CORT、 DA、 NE as part of the micro-indicators on TCM syndrome differentiation.
A-209Study on the relationship between Qi asthenia phlegm-stasis syndrome of the malignant tumor and Immunity Cell and Hormone secretion
Wang Qiaolin1, Du Yeqin 1, Halmurat Upur 2*1Tumor Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China;2Traditional Uighur Medicine
Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; *Correspondence should be
addressed to [email protected]
Objective: To analyse the relationship between Qi asthenia phlegm-stasis syndrome of the malignant tumor patients and Immunity Cell and Hormone secretion. Method: The flow cytometry technology and
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radio-immune technology were utilized to detect patients' serum level of CD3+、CD4+、CD8+、NK cell、 IL-1β、 IL-6、ACTH、CORT、TNF-α of 41 cases of malignant tumour with Qi asthenia and phlegm-stasis syndrome and 42 cases of malignant tumour with non-Qi asthenia and phlegm-stasis syndrome. Result:1) according to Traditional Chinese Medicine dialectics type, the Qi asthenia and phlegm-stasis syndrome was 49.39% ,the non-Qi asthenia and phlegm-stasis syndrome group was 50.61%. 2) ① In Qi asthenia and phlegm-stasis syndrome group and all the non-Qi asthenia and phlegm-stasis syndrome group, CD3+、 CD4+、 CD4+/CD8+、 NK went down, and CD8+ went up .There is statistical difference compared with normal group(P＜0.01 or P＜0.05), while compared with the Qi asthenia and phlegm-stasis syndrome group with the non-Qi asthenia and phlegm-stasis syndrome group in CD3+、CD4+, there is no statistical significance (P＜0.05). IL-1β and IL-6 ② in Qi asthenia and phlegm-stasis syndrome group and the non-Qi asthenia and phlegm-stasis syndrome group decreased significantly compared with normal group (P＜ 0.01 or P＜ 0.05), but there is no obvious difference in both groups(P＞ 0.05). TNF-α decreased ③ in Qi asthenia and phlegm-stasis syndrome group and the non-Qi asthenia and phlegm-stasis syndrome group. Compared with normal group, there is no statistical significance (P>0.05). ACTH and CORT went up ④ in Qi asthenia and phlegm-stasis syndrome group and the non-Qi asthenia and phlegm-stasis syndrome group compared with normal group (P<0.05) (P<0.01), the two groups had no obvious difference (P ＞ 0.05). Conclusion: 1) Qi asthenia and phlegm-stasis syndrome occupied greater proportion in malignant tumour of TCM dialectical type, so Qi asthenia and phlegm-stasis syndrome was one of the important syndrome types of malignant tumour. 2) The change of Immunity Cell and Hormone secretion exist in Qi asthenia and phlegm-stasis syndrome group and the non-Qi asthenia and phlegm-stasis syndrome group, especially in Qi asthenia and phlegm-stasis syndrome group was more outstanding, in which the change was primarily in CD3+、CD4+、CD8+、NK、 IL-1β、ACTH.
A-210The clinical observation of the fixation in the treatment of acute ankle sprain in Traditional Uygur Medicine
Jin Hao1, Lv Faming1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
Objective: To explore the traditional Uygur Medicine Efficacy of the treatment of acute ankle sprains, and evaluate of its clinical advantages and characteristics. Method: According to the Western and TCM Designate diagnostic criteria, by collection, 94 cases of acute ankle sprain were all from orthopedic clinic and orthopedic emergency were randomized, in which 37 cases called observation group using Traditional Uygur external fixation and 57 cases with cast immobilization were called the control group. Two sets of initial therapy with conventional "RICE" method and oral blood circulation of the san qi pill were used. External fixation of the observation group and control group changed once a week, one week course of treatment and continuous observation of the two courses, TCM syndrome score and the AOFAS score, VAS score, and ankle swelling as the efficacy evaluation. Results: there were no significant Difference between the two groups in gender, age, age distribution, the tribes, (P>0.05), the AOFAS score of the two groups were statistically significant compared with the before treatment (P<0.05), there were no significant difference one week and two weeks after the treatment for the point
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in time (P>0.05). TCM syndrome score were compared statistically different (P<0.05). The difference were statistically significant in the VAS pain product and swollen joints before and after treatment (P<0.05).Conclusion: By early using the traditional Uygur Fixation for the treatment of acute ankle sprain, we can clearly achieve the anti-inflammatory swelling, relieve pain in certain geographical areas and can effectively replace the cast immobilization.
A-211The Clinical Research of Acupuncture Combined with Auricular Acupuncture for Patients with Acute Exacerbation of COPD to Improve their Quality of Life and Pulmonary Function
Cao Lin1, Liu Zhiyan1
1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uighur Autonomous Region, China
Objective: To observe the influence of life quality and the improvement of pulmonary function with acupuncture combined with auricular acupuncture therapy on patients with acute exacerbation of COPD. Methods: 60 patients with acute exacerbation of COPD were randomly divided into four groups: acupuncture combined with auricular acupuncture group, acupuncture group (acupuncture combined with simulate auricular acupuncture), auricular acupuncture group (simulate acupuncture combined with auricular acupuncture), control group (simulate acupuncture combined simulate auricular acupuncture).Four groups received conventional treatment as basis combined with acupuncture, auricular acupuncture, simulated acupuncture, simulated auricular acupuncture. After two courses (20 days) of treatment, observe FEV1, FEV1/FVC ratio of pulmonary function and CAT, MRC, as well as traditional Chinese medicine symptom scale quality of life scores of the four groups of patients before and after treatment. Results: The lung function: There was statistically significant (P<0.05) of patients with pulmonary function in four groups before and after treatment. acupuncture combined with auricular acupuncture group’s FEV1 、 FEV1/FVC ratio improved compared with before treatment, the improvement of pulmonary function was better than the other three groups; CAT scales reflect the quality of life score decreased significantly compared with before treatment, acupuncture combined with auricular acupuncture group was better than the acupuncture group and auricular acupuncture group, and was statistically significant compared with control group (P<0.05). Conclusion: Acupuncture combined with auricular acupuncture for treating acute exacerbation of COPD patients with the pattern of phlegm obstructed in lung, which is effective in the clinical symptoms, especially the improvement of airflow limitation as well as difficulty in breathing. To compare the four groups with each other, we suggest that acupuncture, auricular acupuncture can treat COPD, the combination of their two is better, and patients’ quality of life are also improved.
A-212The correlation of TCM syndrome with the BODE index and quality of life for patients with COPD at stable phase
Zhou Wei 1, Mao Bing 1*1Huaxi hospital of Sichuan university of combination of TCM and western medicine department, 610041, Chengdu, China
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Objective: To study the syndrome of TCM (Traditional Chinese Medicine) and relevant indexes of conventional Western Medicine for patients with COPD (chronic obstructive pulmonary disease) at stable phase, and further explore the trend of change of the deficiency syndrome of TCM, and the correlation of each syndrome with the BODE index (body mass index, airflow obstruction, dyspnea, and exercise capacity index) and quality of life. It was anticipated that this study could provide evidence-based information for pattern differentiation, and also promote the combination of TCM and conventional Western medicine in the treatment of COPD at the stable phase. Methods: Based on relevant literature and diagnostic criterion of TCM, five groups of symptoms of TCM about stable COPD were established, which are Lung Qi deficiency , Lung and Spleen Qi deficiency , Lung and Kidney Qi deficiency, Lung, Spleen and Kidney Qi deficiency, and deficiency of both Qi and Yin. 300 cases which are up to the standard were differentiated to 5 groups by the symptoms. Some basic details about the patients were recorded, such as course of disease, time of acute exacerbation every year, weight and height. Also BODE index and COPD assessment test were detected. And then statistic and analyses the differences of the indexes among groups, and the relationship between the BODE index and the CAT (COPD assessment test) score. Results: ①The course of disease in Lung Qi deficiency group was shorter than those in Lung and Kidney Qi deficiency group, Lung, Spleen and Kidney Qi deficiency group, deficiency of both Qi and Yin group (P<0.05); the times of acute exacerbation every year in Lung Qi deficiency group were less than those of the rest four groups (P<0.05).②The BMI of the patients in Lung Qi deficiency group was higher than that in Lung and Spleen Qi deficiency group, Lung, Spleen and Kidney Qi deficiency group, deficiency of both Qi and Yin (P<0.05); the BMI of the patients in Lung and Kidney Qi deficiency group was higher than that in Lung, Spleen and Kidney Qi deficiency group, deficiency of both Qi and Yin (P<0.05). ③The degree of airflow obstruction of patients in Lung Qi deficiency group was mainly concentrated in Ⅱstage, the degree of airflow obstruction of patients in Lung and Spleen Qi deficiency group was mainly concentrated in Ⅲ stage, the degree of airflow obstruction of patients in Lung, Spleen and Kidney Qi deficiency group, deficiency of both Qi and Yin group were mainly concentrated in Ⅲ、Ⅳ stage. The order of FEV1% from high to low was Lung Qi deficiency/Lung and Spleen Qi deficiency/Lung and Kidney Qi deficiency/Lung, Spleen and Kidney Qi deficiency(P<0.05). ④the patients' degree of dyspnea in Lung Qi deficiency group was lighter than that in the rest four groups(P<0.05); the patients' degree of dyspnea in Lung and Spleen Qi deficiency group was lighter than which in Lung, Spleen and Kidney Qi deficiency group and the deficiency of both Qi and Yin group (P<0.05); the patients' degree of dyspnea in Lung and Kidney Qi deficiency group was lighter than that in Lung, Spleen and Kidney Qi deficiency group(P<0.05). ⑤The 6MWD of patients in Lung Qi deficiency group was longer than that in the other four groups(P<0.05); the 6MWD of patients in Lung and Spleen Qi deficiency group was longer than which in Lung, Spleen and Kidney Qi deficiency group and deficiency of both Qi and Yin group(P<0.05); the 6MWD of patients in Lung and Kidney Qi deficiency group was longer than that in Lung, Spleen and Kidney Qi deficiency group(P<0.05); ⑥The patients' BODE index and CAT score in Lung Qi deficiency group is lower than that in the other four groups(P<0.05); the patients' BODE index and CAT score in the Lung and Spleen Qi deficiency group is lower than that in Lung, Spleen and Kidney Qi deficiency and deficiency of both Qi and Yin group(P<0.05); the patients' BODE index and CAT score in Lung and Kidney Qi deficiency group is lower than that in the lung-qi, spleen-qi and kidney deficiency group (P<0.05).⑦The patients' CAT score in Lung and Kidney Qi deficiency group is lower than that in deficiency of both Qi and Yin group; The CAT score of patients in Lung, Spleen and Kidney Qi deficiency group is lower than that in
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deficiency of both Qi and Yin group(P<0.05) ⑧The BODE index were dramatically relative with CAT score. Conclusions: These findings suggest that with the progress of COPD, the functions of multiple Zang-Fu organs could ultimately be affected. The symptom type of TCM for the COPD patients at stable phase may vary from Lung Qi deficiency to Lung and Spleen Qi deficiency, or to Lung and Kidney Qi deficiency, and even lung-qi, Lung, Spleen and Kidney Qi deficiency. Additionally, the BODE index and CAT score become increasingly high. The BMI of patients with Spleen-Qi deficiency is lower than that without Spleen-Qi deficiency. The lung function, the degree of dyspnea and the 6MWD of patients with Kidney-Qi deficiency are lower than that without Kidney-Qi deficiency. As for the BODE index and CAT score, patients with Yin deficiency are superior to that without Yin deficiency. The BODE index score were positively correlated with CAT score.
A-213The effect of Tanreqing injection on lipopolysaccharide-induced airway inflammation, mucus hypersecretion in rats and their own dose-response relationship
Zhou Yunqing 1, Mao Bing 1*1Huaxi hospital of Sichuan university of combination of TCM and western medicine department, 610041, Chengdu, China
Objective: The aim of this study was to observe the role of lipopolysaccharide (LPS)-induced airway inflammation and mucus hypersecretion in rats, and to investigate the effect of Tanreqing injection on airway inflammation, mucus hypersecretion and their own dose-response relationship. Methods: Forty-eight male specific-pathogen-free Sprague-Dawley rats were randomly divided into four groups, with 12 rats in each group: control group, LPS group, and Tanreqing groups (at doses of 2.8 and 5.6 ml/kg/24h, respectively). Control rats were given a 120μl saline through intratracheal instillation. An equal volume of LPS was instilled intratracheally to induce airway inflammation and mucus hypersecretion. Tanreqing injection at 2.8 and 5.6ml/kg/24h was given with an intraperitoneal injection at 1h prior to LPS administration and then once every 24h. Rats from the control and LPS groups received 5.6ml/kg/24h saline instead of Tanreqing injection. Rats were randomly sacrificed by exsanguination at 24h, 48h, and 96h after LPS challenge. The bronchoalveolar lavage fluid (BALF) and the right lung were collected. The number of total cells and neutrophils in BALF were determined. The levels of inflammatory cytokines interleukin-1β (IL-1β), CINC-1, and tumor necrosis factor-α (TNF-α) in BALF were measured using ELISA. The middle lobe of the right lung was stained with hematoxylin-eosin (H&E), and the histological change was examined. BALF was analyzed for total protein using the BCA method and Muc5ac quantification using ELISA. Additionally, airway mucus hypersecretion was also evaluated with Alcian blue/Periodic acid-schiff (AB/PAS) staining, immunohistochemistry, and real time-PCR. Results: In the control group, there were no significant changes in terms of the number of inflammatory cells and the levels of inflammatory cytokines in BALF, the histological change, total protein and Muc5ac quantification in BALF, AB/PAS staining area, and Muc5ac expression. Compared with the control group, LPS induced significant increase in above markers. The inflammatory markers induced by LPS were peaked on 24h, decreased on 48h, and further decreased on 96h. The airway mucus hypersecretion induced by LPS were increased on 24h, further enhanced on 48h, and peaked on 96h. Treatment with Tanreqing injection significantly reduced the number of the LPS-induced inflammatory cells, the levels of inflammatory cytokines in BALF, histopathological change, total protein in BALF, AB/PAS staining area, and Muc5ac expression. Furthermore, high-dose of Tanreqing injection shows
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increasing benefits over low dose, but the difference was not statistically significant. Conclusions: The LPS-induced airway inflammation were peaked on 24h, decreased on 48h, and further markedly decreased on 96h. The LPS-induced airway mucus hypersecretion were increased on 24h, further enhanced on 48h, and peaked on 96h. The present results suggest that Tanreqing injection is endowed with anti-inflammatory properties and can effectively inhibit the airway mucus hypersecretion. In addition, Tanreqing injection inhibits the airway inflammation and mucus hypersecretion in a dose-dependent trend. However, their own dose-response relationship and involved molecular mechanism of Tanreqing injection have not been elucidated.
A-214The Modern Medicine and Traditional Uighur Medicine research of neuro-endocrine-immune in malignancies
Zhang Li 1, Halmurat Upur 2*1Tumor Center of the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China; 2Traditional Uighur
Medicine Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to [email protected]
Objective: 1) To investigate the disorder of neuro-endocrine-immune network in patients with cancer and its clinical significance. 2) To investigate clinical types of malignancy in Traditional Uighur Medicine and the disorder of neuro-endocrine-immune network. 3) In order to investigate the alteration of some indexes about neuro-endocrine-immune in different model types, the abnormal savda syndrome transplanted tumor model was established on the basis of the abnormal savda syndrome model. In order to research further the relevance between the abnormal savda syndrome tumour and the disorder of neuro-endocrine-immune, the morphological and ultrastructural alteration of hypothalamus -pituitary-adrenal axis was observed. Methods: 1)102 patients with histological proven cancer were enrolled in the study. Radio-immune technology and flow cytometer technology were utilized to detect blood levelofCD3+,CD4+and CD8+, and cytokines including IL-1β, IL-6,TNF-α,ACTH, CORT and DA as well as NE; And meanwhile analyses were performed on the basis gender, pathology, metastasis status, and performance status and treatment pattern. 30 normal people were served as control group. 2) Radio-immune technology and flow cytometer technology were utilized to detect the serum level of CD3+、CD4+ and CD8+, and cytokines including IL-1β、 IL-6、TNF-α、ACTH、CORT、DA and NE. 3) 120 ICR mouse were divided randomly into four model groups namely group control, group tumour, group abnormal savda syndrome model and abnormal savda syndrome tumour model. After the abnormal savda syndrome model was established, S180 ascites tumour cells were transplanted subcutaneously into the mouse thigh, then macroscopic tumour mass were formed. The serum level of IL-1β, IL-6, TNF-α, ACTH and CORT were examined by ELISA. The structural change of hypothalamus, pituitary and adrenal were observed via using microscope and electronic microscope to calculate the rate of transplantation and to weigh the tumour mass. Results: 1) A significant decrease of CD3+, CD4+ lymphocytes count, CD4+/CD8+ and a significant increase of CD8+lymphocyteswere found in almost all the cancer patients compared with the control group(P<0.05 or P<0.01). Cancer groups had lower IL-1βand TNF-α level, l but falling in lung cancer group and head and neck group were significant (P<0·05). Various cancer types had lower NK level than in normal group (P<0·05). The serum CORT level increased only in gastrointestinal cancer patients (P<0.05); The ACTH level had no difference
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among all groups. Both serum DA and NE level were increased in almost all cancer groups compared with the normal group (P<0·05 or P<0·01). 2) Decrease ofCD3+lymphocytes in patients treated by chemotherapy and irradiation therapy vs non-CT/RT patients were significant (P<0·05). In RT group the higher level ofCD8+and lowerCD4+/CD8+was significant than non-CT/RT (P<0·05). The level of IL-1β and Il-6 in CT group was higher than RT group (P<0·05); but the latter had higher TNF-α level (P<0·05). (3)In female patients, CD3+and CD8+lymphocytes, IL-1βand TNF-α were increased significantly than in male patients (P<0·05). (4)Our data presented that IL-6 was significantly increased in patients with cancer and mestastic ones (P<0·05). (5)The poor performance status patients had higher CORT and TNF-α (P<0·05); The level ofCD4+/CD8+in patients in the late stage was significantly lower than in the early stage patients (P<0.05). 2)①Patients with Abnormal Savda Syndrome cancer occupied 72% in all patients; non-abnormal Savda Syndrome cancer was 28%. ②A significant decrease of total and CD3+ and CD4+ lymphocytes count was found in abnormal savda syndrome cancer patients compared to both the control group(P＜0.01)and non-Abnormal Savda Syndrome cancer(P＜0.05). A significant increase of, the abnormal savda syndrome and non-abnormal savda syndrome had higher CD8+ compared with the control group(P＜0.05), the former was more obvious. A significant decrease of CD4+/ CD8+ was found out in both two tumor groups(P＜0.01),but the abnormal savda syndrome was little obvious. The two tumor groups had lower level NK (P＜0.05), but no significant difference between them (P＞ 0.05). ③Two cancer groups had lower IL-1β level, but falling in non-abnormal Savda Syndrome cancer group was significant (P＜0.05). Compared to the control group, IL-6 and ACTH was significantly increased in patients with in abnormal savda syndrome cancer and non-abnormal savda syndrome cancer, but no difference between them. TNF-α was increased significantly only in Abnormal Savda Syndrome cancer patients (P＜ 0.05). The serum level of CORT in the abnormal savda syndrome cancer present significant increasing than non-Abnormal Savda syndrome cancer (P＜0.05). ④A significant increasing of DA in serum in both abnormal savda syndrome cancer and non-abnormal savda syndrome cancer(P＜0.01). The serum level of NE was increased significantly in non-abnormal savda syndrome cancer compared with abnormal savda syndrome cancer (P＜0.05). 3)①In the state of abnormal savda syndrome, the rate of tumor transplantation was increased significantly than the normal state, respectively 100% and 63.3% (P＜0.01); and the weight of tumor mass was higher in former than in later (P＜ 0.01). ②Under microscope, compared with the normal group, cells of the hypothalamus were characterized by the glial cells hyperplasia, neurocells edema, vacuolus degeneration in all three model groups, but the group abnormal savda syndrome tumor model presented more sever than other two groups. Pituitary cells hyperplasia and vascular dilation were similar in three model groups. ③All the three model groups had HPAA ultrastructural abnormality including cell nucleus malformation, perinuclear cisterna enlarged, heterochromatin aggravation, cell organelle falling and so on, especially the abnormal savda syndrome tumor model was the most serious, and the abnormal savda syndrome group was second one, the tumor model was relatively mold. ④Composed with normal group, both abnormal savda syndrome tumor model and abnormal savda syndrome group the serum level of ACTH were increased and CORT deceased, IL-6 also increased (P＜0.05), but no difference between them(P＞0.05). TNF-α was raised in abnormal savda syndrome group than in tumor group (P＜0.05). There was no different between four groups to IL-1β. Conclusion: 1) The disturbance of neuro-endocrine-immune network in patients with cancer is characterized by increase of the serum level of TNF-α, IL-6, CORT, DA and NE and decrease of total CD3+, CD4+ and CD4+/CD8+ lymphocytes; Moreover it is related to the gender, pathology, mestastic status and treatment type; not related to the PS. 2) Abnormal Savda syndrome body fluid facilitated
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tumour development, a phenomenon that might be related to the neuroendocrine-immune disorder. HPAA damages in abnormal Savda syndrome tumour model were more severe than the other experimental groups, suggesting that abnormal Savda syndrome body fluid and the tumour might have a synergistic effect on HPAA damages. The disturbance of the neuroendocrine-immune axis in abnormal Savda syndrome tumours was more severe as shown by the morphological data.
A-215The Observation of Curative on Extracorporeal High-Frequency Hyperthermia Combined with Yangzhengxiaoji Decoction ’s Treatment of Advanced Non-small-cell Lung Cancer
Wu Shanshan 1, Zhang Hongliang 1
1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang Uighur Autonomoes Region, China; *Correspondence should be addressed to [email protected]
Objective: Using pilot study method of the clinical non randomized controlled, patients with advanced non-small cell lung cancer(NSCLC) were treated with prospective study of Integrative Chinese and Western Medicine, whose ages were between 30-85 years old. To observe the combined treatment efficacy and advantages of quality of life. Methods: 92 advanced NSCLC patients who visited the TCM hospital of Xinjiang Autonomous Region and the TCM hospital of Urumqi city were divided into two groups by a randomized controlled pilot study medthod. The test group: extracorporeal high-frequency hyperthermia combined with Yangzhengxiaoji decoction; the control group: simple Yangzhengxiaoji decoction, 46 cases in each of group. In the four weeks of treatment, we recorded every patients’ baseline indexes, tumor lesions change, KPS score, symptom score and quality of life score. Results: Of the 92 patients, 87 patients completed test, 5 patients were out of the groups. The two groups had same levels in tumour body stable rate and reduced TCM hospital syndrome evaluation(P>0.05). The treatmeat group was better than the control group on improving patients with KPS score. Analysing the quality of life, functional area: two groups of patients have same improved degree of role function, emotional function, social function, general health status of the functions(P>0.05); The treatment group patients were improved with the physical function, cognitive function, and the improved physical function is better than that of control group (P<0.05); improvement of the cognitive function compared with the control group had no significant.(P>0.05); Symptoms: the two groups had the improved patient’s symptoms in the side of pain, dyspnea and lung specific symptoms ( including shortness of breath, cough, hemoptysis, pain ). The curantive effects in treatment group was better than that in control group(P<0.05); the two groups had improved the patients’ fatigue symptom, but none of obvious difference(P>0.05). The two groups increased the cost of patients, and the treatment group was significant, but none of obvious differences(P>0.05). Conclusion: (1) External high frequency thermotherapy combined with Yangzhengxiaoji Decoction method could improved patients’ physical condition, peoples’ quality of life by the improvement of pain, dyspnea symptoms; (2)This program could be one of the treatment scheme selections on non-small-cell lung cancer.
A-216The relationship between Bronchial asthma TCM syndrome differentiation and their NE、DA、5-HT changes
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You Lina 1, Halmurat Upur 2*1 Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous RegionChina; 2Traditional
Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;
*Correspondence should be addressed to [email protected]
Objective: To investigate the change between bronchial asthma patients' plasma monoamine neurotransmitter and their TCM syndrome differentiation. Methods: Randomly collect 84 cases of bronchial asthma patients from the respiration department in the Affiliated Traditional Chinese Medicine hospital of Xinjiang Medical University. Divided them into different groups according to TCM syndrome differentiation. The contents of plasma monoamine neurotransmitter were measured by high perfonmence liquid chromatography (HPLC), such as NE、DA and 5-HT.46 normal people were served as control group. Results: In total clinical cases, the ratio of kidney deficiency and stagnation of phlegm was 46%, the non- kidney deficiency and stagnation of phlegm was 54%(contain the ratio of cold phlegm was 21%, the ratio of heat phlegm was 15%, the ratio of frigiopyretic phlegm was 7%, the ratio of anemophlegmatic phlegm was 11%).The kidney deficiency and stagnation of phlegm mean age is greater than the non- kidney deficiency and stagnation of phlegm. And the two of group didn't present statistical significance in gender (p>0.05).The content of NE and DA in bronchial asthma patients was higher than the normal control group (p<0.05). Inter-compared with kidney deficiency and stagnation of phlegm and non- kidney deficiency and stagnation of phlegm and control group, the content of NE and DA in three groups present statistical significance. compared to the normal control group, NE and DA was significantly decreased in kidney deficiency and stagnation of phlegm(p<0.05),but in non-kidney deficiency and stagnation of phlegm, the content of NE and DA didn't present statistical significance(p>0.05).A significant increase of NE and DA was found in non-kidney deficiency and stagnation compared to kidney deficiency and stagnation(p<0.05),two groups had no statistical significance in 5-HT(p>0.05). Conclusions: 1) Bronchial asthma patients' plasma monoamine neurotransmitter are disordered, asthma can cause the increase of neurotransmitter, or nervous reflex could aggravate symptoms of asthma. 2) “The same disease different syndrome, the different syndrome different principle”. Kidney deficiency and stagnation of phlegm was the most common type of bronchial asthma. There is variability in the release of neurotransmitter because of the difference of TCM syndrome differentiation. The lower content of NE and DA in bronchial asthma patients of kidney deficiency and stagnation of phlegm, it is maybe excitability of central nervous system to lower, so the release of monoamine neurotransmitter to be depression. This is maybe a part of biology foundation of “deficiency in origin” in asthma of kidney deficiency and stagnation of phlegm.
A-217The relationship between tumor patients’ plasma monoamine neurotransmitter changes and their Traditional Uighur Medicine syndromes
Chen Qingying1, Du Yeqin 1, Halmurat Upur 2*1 Affiliated Cancer Hospital of Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region ， China; 2Traditional Uighur Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
Objective: To determine the relation between tumor patients’ plasma monoamine neurotransmitter and
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their Traditional Uighur Medicine types. Methods: Grouped the patients according to their Traditional Uighur Medicine syndromes. The contents of plasma monoamine neurotransmitter were measured by high perfonmence liquid chromatography (HPLC), such as NE, DA, 5-HT and DOPAC et al. Results: Compared with venign tumour group, the percentage of Malignancy abnormal savda syndrome was markedly highly than the other three abnormal Traditional Uighur Medicine syndrome. The contents of plasma 5-HT have significantly difference between malignancy and venign tumour patients. While the contents of plasma NE and the levels of NE/DA are significantly different between abnormal savda syndrome patients and the other Traditional Uighur Medicine syndromes. Conclusion: The above results suggest that the content change of NE and the level of NE/DA may be indexes to diagnose the malignancy tumour belonging to abnormal savda syndrome. In a word, the plasma monoamine neurotransmitters changes and the imbalance among them are related to the Traditional Uighur Medicine syndromes.
A-218The study between IFN-γ methylation and Chinese medicine syndrome type in subsets of allergic rhinitis patients between Uighur and Han in Ili of Xinjiang
Zhang Qingquan 1, Gao Li 1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
Objective: To explore the expressions of IFN-γ gene methylation and Chinese medicine syndrome type between Uygur and Han population in patients with allergic rhinitis in Ili of Xinjiang. Through epidemiology investigation of allergic rhinitis get distribution rule of Chinese medicine syndrome type. To analysis the correlation between serum IL-4 , IFN-γ and IgE level and nasal mucosa eosinophilic. Methods: AR patients and normal group were randomly surveyed from the crowds through allergic rhinitis epidemiological survey in Ili of Xinjiang. To extract genomic DNA from peripheral venous blood and modified DNA by bisulfit emodification and adopt methylation specificity polymerase chain reaction(MSP) for the analysis of methylation state.Through double-antibody Enzyme-linked Immunosorbent Assay (ELISA), 59 cases Uygur AR patients, 50 patients Han’s AR serum IL-4 and IFN-γ levels were analyzed, pathology smear was observed and nasal smear mucosa eosinophil was counted at high-power microscope. Results: The positive rates of IFN-γ gene methylation of Han healthy control group(16.9%)were lower than in Uygur healthy control group(1.7%)(P<0.05). IFN-γ hemimethylation rate of Han healthy control group(96.6%) was higher than in Uygur healthy control group(79.7%)(P<0.01). It not only had no significant differences between Han AR patients(8.5%) and control group(1.7%) but also between Uygur AR patients(18.6%) and control group (16.9%) in positive rate of IFN-γ gene methylation (P>0.05). There were no significant differences between Uygur AR patients (18.6%) and Han (8.5%) of IFN-γ gene methylation (P>0.05). The highest Chinese Medicine syndrome type of deficiency of lung qi(69.01%),followed by deficiency of spleen qi type(13.22%)，syndrome of lung heat(9.51%),syndrome of deficiency of kidney(8.26%). IL-4 and IgE levels and nasal eosinophil counts were positively correlated in Xinjiang Uygur and Han population in patients with AR (P<0.01), IFN-γ levels and nasal eosinophil count was negatively correlated (P<0.01); Conclusion: The positive rates of IFN-γ gene methylation of Han healthy control group were lower than in Uygur healthy control group, but it had no significant differences between AR patients and control
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group, it will provide us with many benefits to our future research. In Chinese medicine syndrome type, deficiency syndromes in lung type higher than the other three types, Climate and environment have a certain influence on AR. For Uygur and Han people, excess secretion of serum IL-4, IgE and inhibition secretion of IFN-γ were important factors in allergic rhinitis nasal eosinophil counts production.
A-219The study of the improvement of clinical effect about “Reinforcing Qi and Activating Blood” in Stable Chronic Obstructive Pulmonary Disease (Qi Deficiency and Blood Stasis Syndrome)
Min Jie 1, Mao Bing 1* 1Huaxi hospital of Sichuan university of combination of TCM and western medicine department, 610041, Chengdu, China
Objective: Under the direction of “Reinforcing Qi and Activating Blood”, we studied the TCM clinical effect on patients with stable chronic obstructive pulmonary disease (Qi Deficiency and Blood Stasis Syndrome), who were administered with Yu-Ping-Feng capsules and Fu-Fang-Dan-Shen tablets. We finally assessed the improvement of TCM clinical symptoms, evaluated the effect of TCM syndrome, and determined the TCM clinical curative effect through comparing with experimental datas and the end-points. Method: A randomized controlled trial (RCT) was designed. Fifty outpatients with stable COPD of Qi Deficiency and Blood Stasis Syndrome were randomly and averagely allocated to control group and treatment group. Patients in the study group were given both health education and the treatment of Yu-Ping-Feng capsules and Fu-Fang-Dan-Shen tablets for 3 months, while patients in the control group were only given health education for 3 months, then all patients were followed-up for 12 months. We calculated the score of TCM symptom before the treatment and at several time points after the treatment (1.5 months after , 3 months after, 6 months after , 9 months after and 12 months after), we evaluated the curative effect of TCM syndrome at the five time points and observed the level of the cellular immunity criterias, including CD4 、 CD8、 CD4/CD8 and the haemodynamics criterias, including Nitrogen Monoxidum(NO) and Endothelin(ET). We also assessed the frequency of the cold, the number of times admission due to acute exacerbation and the annual days of hospital stay. Results: (1)Baseline levels: There were no statistical significance in gender, smoking, age, course of disease, symptom scores of TCM, between the two groups at baseline (all P>0.05).（ 2） TCM symptoms: The improvement value in the treatment group —the total symptom scores of TCM at 1.5 months and 3 months after the treatment minus the total symptom scores of TCM symptom before the treatment—was higher than that in the control group (P<0.05). However the improvement value between two groups —the score of TCM symptom at 6 months，9 months and 12 months after the treatment minus the score of TCM symptom before the treatment—was not significantly different（P>0.05）,but the level of the reducing scores in the study group was higher than that in the control group. The symptom of shortness of breath was improved obviously at 3 months after the treatment in the treatment group (P<0.05), and the symptom of spontaneous sweating was improved obviously at 1.5 months, 3 months and 6 months in the treatment group (P<0.05). The symptoms of lack of strength, fatigued spirit, thoracic oppression and shortage of Qi, were improved at most time points in the treatment group（P<0.05）.（3） the Curative Effect of TCM Syndrome: The total effective rates in the treatment group at the five time points were 23.81%、57.14%、38.10%、28.57%、19.05% respectively, and the total effective rates in the control group at 5 different time points were
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5.00%、10.00%、10.00%、15.00%、10.00% respectively. The total effective rate in the treatment group at 3 months and 6 months after the treatment was higher than that in the control group（P<0.05）.(4) Experimental Data: The levels of CD4、CD8 and the ratio of CD4/CD8 were not significantly different（P>0.05） ; In the treatment group, the level of CD4 and the ratio of CD4/CD8 after the treatment was higher than that before the treatment（P<0.05） , and the level of CD8 was lower than that before the treatment（P<0.05）. In the control group the levels of CD4、CD8 and the ratio of CD4/CD8 were not significantly different（P>0.05） . The level of NO between the treatment group and the control group had no significant difference（P>0.05） , while the level of ET in two groups was significantly different (P<0.05). Specifically, in the treatment group the level of NO after the treatment was higher than that before the treatment（ P<0.05） , while the level of ET after the treatment was lower than that before the treatment（P<0.05）（5）the End-points: The frequency of the cold, the number of times admission due to acute exacerbations and the annual days of hospital stay in the study group were all lower than those in the control group（P<0.05） .Conclusion: The method of “Reinforcing Qi and Activating Blood” can improve symptoms and clinical curative effect of TCM in the treatment of stable COPD (Qi Deficiency and Blood Stasis Syndrome). It also can improve their cellular immunological function and vasomotor function. And it can shorten the frequency of the cold, the number of times admission due to acute exacerbation and the annual days of hospital stay as well.
A-220The Study on Levels of Immunoglobulin and Complement in Henoch-schonlein Purpura Patient with Different Syndrome
Zhang Cheng1, Bai Yusheng 1
1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang Uighur Autonomous Region, China
Objective: This paper is to study the correlation between HSP patients with different TCM syndrome type and Immunoglobulin and Complement 3,4 so as to supply the theory basis for objectify of TCM syndrome type in HSP patients. Methods: established the perfect symptoms observation charts, and selected the patients in accord with diagnostic criteria and standards criteria, then collected the TCM manifestation of patients without treatment whom in acute stage. Divided the selected HSP patients into 4 basic TCM syndrome type; At the same time, test the serum immunoglobulin andcomplement testing. Results:(1) 112 cases of patients with selected HSP, men 53 cases (47.32%), women 59 patients (57.68%), male: female = 1:1, maximum age 71 years, minimum for 4 years, mean age 23.71 ±15.80 years old, men and women the disparity between the sexes was not statistically significant.(2). By comparing HSP type certificate of the patients with average age, the comparison between syndrome types, wind hot injury type winding minimum average age, and the average age was taken blood type relative to other group is the largest, each card type average age difference was statistically significant (P>0.05); 3.The group of 112 cases of patients with HSP before treatment, wind hot injury type winding 32 cases (28.57%); Blood hot injury of 38 cases of type winding (33.93%); Yin deficiency type fire 24 cases (21.43%); Qi not type taken 18 cases (16.07%). 4.Immune globulin IgA level, blood hot injury type winding group higher than other groups, and the rest of the three groups compared respectively, P<0.05; IgG test results spirit not taken blood type higher than other three groups, Yin deficiency group
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compared with fire, P<0.05; IgM test results, wind hot injury type winding level higher than the other group, with the rest 3 groups were compared, P<0.05; C3, C4 results Yin deficiency fire type are all lower than those of other groups, including the C3 level detection, Yin deficiency fire type group was lower than other groups, the blood hot injury type winding group level is the highest, Yin deficiency fire with blood type heat injury type winding, wind hot injury type winding comparison group, P<0.05; C4 level detection, Yin deficiency fire type group level lower than other group, wind hot injury type winding highest group, Yin fire with the other three groups of comparison, P<0.05. Conclusion:1.the rise of Immunoglobulin IgA has reference value to judge HSP patients with blood heat syndrome type; while the same on IgM to wind heat syndrome type and IgG to yin deficiency fire excess syndrome type; 2.The reduce of Serum C3, C4 level tend to yin deficiency fire excess syndrome type in chronic stage; 3.There were differences on Immunoglobulin and complement in HSP patients with various types which provides theory basis and reference.
A-221The study on the imbalance of Regulatory T cells/Th17 cells in the different Traditional Chinese Medicine syndromes of asthma
Yan Lu 1, Zhou Xianmei 1
1Nanjing University of Traditional Chinese Medicine is affiliated with the Chinese Medicine Hospital of Jiangsu Province,
210029, Nanjing, China
Objective: Bronchial asthma is divided into different syndromes in Traditional Chinese Medicine (TCM). The imbalance of Regulatory T (Treg) cells/Th17 cells differentiation plays an important role in asthma. Treg cells and Th17 cells express interleukin (IL-10) and IL-17A, respectively. The current study is to evaluate Treg cells/Th17 cells differentiation via detecting the IL-10/IL-17A levels in serum, explore immunifaction of different syndromes in TCM, provide objective proof for TCM syndromes, and further benefit the clinical therapy. Methods: 15 patients of hot type asthma, 15 patients of deficiency syndrome of kidney yang, and 15 healthy volunteers were involved. Blood samples were collected from the subjects, serum was separated and stored at -80 oC. IL-10 and IL-17A levels in the samples were detected with enzyme-linked immunosorbent assay (ELISA) method. Results: 1. The levels of IL-10 of both hot type asthma (64.76±8.18) and kidney yang-deficiency (57.62±12.94) groups were decreased compared with that of volunteers group (104.22±42.07) (P<0.01). 2. Compared with that of volunteers group (50.26±9.84), the IL-17A level of hot type asthma group (62.05±13.25) was significantly increased (P<0.01), while, there was no statistical difference between the hot and kidney yang-deficiency types. 3. The ratios of IL-10 and IL-17A in hot type asthma (1.08±0.20) and kidney yang-deficiency (1.11±0.37) groups were decreased compared with that of volunteers group (2.10±0.90) (P<0.01). There was no statistical difference between the hot type asthma (1.08±0.20) and the kidney yang-deficiency asthma. Conclusion: 1. The hot type asthmas showed a decrease of IL-10, overexpression of IL-17A, and a reduced ratio of IL-10 and IL-17A, suggesting an imbalance of Treg cells/Th17 cells sourced from a simultaneous IL-10 decrease and IL-17A increase. 2. The asthmas with kidney yang-deficiency types displayed a lower level of IL-10, normal level of IL-17A, and a decreased ratio of IL-10 and IL-17A, also suggesting an imbalance of Treg cells/Th17 cells, but originated from an IL-10 decrease. 3. The results revealed the asthmas with hot type and kidney yang-deficiency type have some abnormal immunifaction, but the IL-10/IL-17A or Treg cells/Th17 cells cannot be an objective
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proof for TCM syndromes.
A-222To study allergic rhinitis of TCM and IL-4 gene methylation between Uighur nationality and Han people comparative in Ghulja of Xinjiang
Liu Futai 1, Gao Li 1*1Traditional Chinese Medicine Institute, 1Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China;*Correspondence should be addressed to [email protected]
Objective: Allergic rhinitis syndromes in Ghulja area distribution and allergic rhinitis IL-4 comparison of gene methylation between Uighur nationality and Han people. Allergic rhinitis in the region get TCM distribution, whether IL-4 gene methylation have difference between Uighur nationality and Han people. Methods: Using random cluster sampling method and survey of AR patients with TCM syndrome differentiation to obtain the prevalence of AR and the constituent ratio of TCM. To sure Uighur nationality and Han people, which have healthy control and patients, extracted from peripheral blood, extracted DNA in the blood and DNA were modified sulfite for IL-4 MSP amplification, and analyse of the results. Results: Investigated 2413 people, including 421 patients AR, the prevalence was 17.45%. Uighur nationality prevalence was 13.76%, which was less than that in Han people (27.43%), P<0.01.It was AR in 446 patients that in line with the number of TCM 242.The highest TCM of syndrome of deficiency of lung qi (69.01%), followed by deficiency of spleen qi type(13.22%), syndrome of lung heat(9.51%), syndrome of deficiency of kidney(8.26%). The IL-4 promoter methylation was 51.61% (16/31) in Han AR, unmethylated was 16.13%(5/31), methylation and unmethy lated was 32.26% (10/31). The complete methylation was 25.81%(8/31) of healthy Han, unmethylation was 6.45%(2/31), methylation and unmethylated was 67.74% (21/31). The IL-4 promoter methylation was 48.38% (15/31) in Uygu AR, unmethylated was 25.81%(8/31), methylation and unmethylation was25.81% (8/31).The complete methylation was 19.35%(6/31) of healthy Uygu ,unmethylation was 38.71% (12/31), methylation and unmethylated was 41.94% (13/31).The AR methylation compared with the control groups of Han people (P<0.05), The AR methylation compared with the control groups of Uighur nationality (P>0.05).It was no difference of AR between Han and Uighur nationality (P>0.05),There have significant differences with healthy control of methylation complete between Han people and Uighur nationality(P<0.01). Conclusion: The prevalence of AR in Uygu nationality, which was less than that in Han people, deficiency syndromes in lung type than the other three types. Climate and environment that have a certain influence on the AR. There have some influence on the incidence between Uighur nationality and Han people living habits and environment. It is no difference between the two nations AR positive, but in Han people have a difference in IL-4 gene methylation. It was a feasible approaches as the study of allergic rhinitis that IL-4 gene methylation. The methulated and unmethylated of Uighur healthy controls which was less than Han. The IL-4 methylation of Han people might tend to expression which susceptible to allergic rhinitis.
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A-223To study the relative of Kidney asthenia blood stasis type deafness and mtDNA 12s rRNA gene mutation in deafness of Uighur ethnic in Xinjiang region of China
Sun Xuefen1, Li Yanhua1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region,
China; *Correspondence should be addressed to [email protected]
Objective: To study the relation of Kidney asthenia blood stasis type deafness and mtDNA 12srRNA gene mutation, the normal people and sensorineural hearing loss in patients in the Uighur nationality, and compare the differences between the two groups; To study the relevance between mutation of deafness with kidney deficiency and blood stasis with mtDNA12S rRNA. Methods: 137 deaf patients and other 93 normal person had been gathered, 137 cases which tinnitus and deafness with differentiation of symptoms and signs for classification of syndrome were divided into kidney asthenia blood stasis (105 cases) and non-blood stasis Kidney and Blood(32 cases) to obtain the blood from the 230 samples people, the mtDNA12srRNA were examined by polymerase chain reaction(PCR), and statistics analysis the relation between Kidney asthenia blood stasis type deafness and mtDNA12srRNA. Result: Many types the mutation had been detected of mtDNA12srRNA and the mutation ratio of A1555G was 4/105(3.8%) higher in patients with hearing loss than the average. The
disease-causing mutations T961cn 、 A827G、nt955ccccnt960 were detected. All of them were not
detected in the normal person. Many types polymorphism the mutation had been detected of
mtDNA12srRNA: G709A、A750G、A1438G and they bear out the mtDNA mutation polymorphic. We
also found a new case of mutant forms: nt950cccccnt956, the pot nt950cccccnt956 may be the new disease-causing mutations type. The differences between Hearing loss group and by mutations in the control group were statistically analyzed, The results showed that the difference was statistically significant,(P<0.05). There were statistically significant differences between Kidney asthenia blood stasis type and mtDNA12S rRNA gene mutation , There was significant difference (P<0.05), that is Kidney asthenia blood stasis type in Blood Uighur has the relationship with mtDNA12S rRNA gene mutations. Conclusion: There were mutually relation between the type of kidney asthenia blood stasis and mtDNA 12srRNA gene mutation in Uighur patients with hearing impairment in Xinjiang. Kidney asthenia blood stasis type might be the reason of mtDNA 12srRNA gene mutation in the Xinjiang region, and there were certain ethnic and geographical characteristics. That carry mutations in the gene for deaf individuals with disease susceptibility, so scientific intervention will help reduce or delay the hearing loss diseases.
A-224Oncothermia combination with Traditional Chinese Medicine: network approach
Hegyi1 Szasz2 A, G. Andocs3 G. Szasz1,4,5 1Department of Complementary Medicine, Medical School, University of Pecs, Pecs, Hungary; 2Department of Biotechnics,
Faculty of Engineering, St. Istvan University, Budapest, Hungary, 3Department of Pharmacology and Toxicology, Faculty of
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Veterinary Science, St Istvan University, Budapest, Hungary; 4OncoTherm Kft. Paty, Hungary; 5OncoTherm GmbH, Troisdorf,
Germany;
Background: Acupuncture and their connective pathways the meridians are ancient Chinese knowledge but it is not understood yet in details. Request of the stable homeostasis of the complex organisms is demanding interdisciplinary approach and new paradigm for the topic. The detecting and reconstructing the deviation from the normal balance of the homeostasis is the basic principle of TCM. The Chinese herbs, the physical (mechanical or electromagnetic acupuncture, acupressure) and mixed forms of heating and diffusion-therapies (moxa therapies) approaches are pointing these problems, and solving it with ancient methods. Oncothermia method (OTM) uses also the deviations from the normal homeostasis for selecting the tumor cells and on this basis ignite natural processes to eliminate them from the system, reestablishing the communication harmony between the cells. This technique is well proven from the laboratory level to the clinical applications. Our aim is to synergize the TCM with OTM on the common basis of equilibrium demand; and use the recognition of the deviations from the complex harmony of the organism or its part for selection to act properly. Method: An extended network approach was presented in the topic, and we would like to continue our research on this basis. The in silico studies will have their roots from the network analysis together with the modern fluctuation theory for complex living organisms (fractal-physiology) was developed in the last decades to study this complexity: like self-organization , fractal physiology, and the bioscaling. Oncothermia widely using these new scientific results; as well as the resonance phenomenon is studied and used in the light of a new theory, and special vector-potential theory helps to complete the method. The problems of the thermal limit in the deep-seated tissues is theoretically and experimentally solved, so it has no any barrier for the wide investigations in synergy experiments. TCM involves electro-acupuncture and laser acupuncture, which are similar in their electromagnetic (conductive) approach to oncothermia effects. The in vivo studies will be performed using a BalbC/nu/nu mice xenografts; using specialized experimental setup (EHY110, Oncotherm, Germany). The effects will be studied histomorphologically (HM) and immunhistochemically (IHCH) by various antibodies with state-of-art high tech digital microscopy system (MiraxWiev, 3D Histech). Conclusion: Recognition of the distortions in the healthy tissue have some common principles and possibilities in TCM and OTM. The synergy of the ancient knowledge and the high-tech state-of-art of the medical knowledge could be established with this research.
A-225Airway inflammation and hypothalamic-pituitary-adrenal axis function in kidney-yang deficiency type of Chinese medicine in asthma and chronic obstructive pulmonary disease
Du Yijie1, Li Bei 1, Cao Yuxue 1, Liu Baojun 1, Wu Jinfeng1, Zhang Hongying 1, Jin Hualiang 1, Dong Jingcheng 1*1Lab of Integrative Medicine for Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, Shanghai 200040,
China;
Correspondence to: Jing-cheng Dong, M.D., Ph.D, Professor of Lab of Integrative Medicine for Lung, Inflammation and
Cancers, Huashan Hospital, Fudan University, Shanghai 200040, China (Tel: 86-21-52888301. Fax: 86-21-52888265. Email:
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Background: Bronchial asthma (BA) and chronic obstructive pulmonary disease (COPD) are both inflammatory airway diseases with different characteristics, but they are often involved with kidney-yang deficiency type of Chinese medicine(KYD). Our study aimed to evaluate changes of inflammatory airway features and the hypothalamic-pituitary-adrenal (HPA) axis function in KYD-BA compared with KYD-COPD. Methods: Thirty-six adults with KYD-BA, 37 with KYD-COPD, 30 with non-KYD-BA and 26 with non-KYD-COPD were recruited in our study. Forty-one healthy individuals were enrolled as controls. Sputum was induced and blood samples were collected for measurement of cytokines and other inflammatory factors. The 24-hour collection of urine was performed and salivary samples for the diurnal rhythm profiles were obtained for assessment of the HPA axis activity. Results: Serum and sputum IgE, and sputum IL-2 in the KYD-BA group were higher than in the healthy control group (P<0.05). Serum IL-2 and IL-6, and sputum IL-6 and TNF-α in the KYD-COPD group were higher than in the healthy control group (P<0.05). Sputum eosinophil cationic protein(ECP) concentrations were significantly higher in KYD-BA group compared to other groups (P<0.001). Serum IL-6, serum TNF-α, and sputum TNF-α in KYD-COPD group were higher than in KYD-BA group (P<0.05). The 24-hour urinary 17-hydroxycorticosteroids/17-ketosteroids concentrations were lower in KYD-BA and KYD-COPD groups than in healthy control group (P<0.05). The peak level and baseline level of the diurnal rhythm were also significantly lower in KYD-BA and KYD-COPD groups compared to other groups (P<0.001). Conclusions: In this study of KYD-BA and KYD-COPD patients, we found that the features of inflammation in KYD-BA and KYD-COPD groups were enhanced. The HPA axis functions in these groups are disturbed or impaired more severely, which may be involved in the pathogenesis of KYD.
A-226Baicalin restores corticosteroid sensitivity in cigarette smoke induced inflammation by modulating HDAC2 in vivo and in vitro
Li Lulu 1, Wu Jinfeng 1, Liu Baojun 1, Sun Jing 1, Gong Weiyi 1, Lv Yubao1, Zhang Hongying 1, Luo Qingli 1, Wu Xiao 1, Dong Jingcheng 1*1Department of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China;
*Corresponding author: Tel.: +86 21 52888301, Fax: +86 21 52888265, E-mail address：[email protected]
Chronic obstructive pulmonary disease (COPD) is a common chronic inflammatory disease of the lungs with little or no response to corticosteroids and a high level of oxidative stress induced by cigarette smoke. In this study, we investigated the mechanism underlying the restoration of corticosteroid sensitivity in cigarette smoke induced inflammation by baicalin, a flavonoid compound isolated from the root of Scutellaria baicalensis Georgi, in vivo and in vitro. In vivo, mice were exposed to smoke of 15 cigarettes for 1 h/day, 6 days/week for 3 months and dosed with baicalin (25, 50 and 100 mg/kg) or dexamethasone (1 mg/kg). In vitro, A549 cells were incubated with baicalin (10, 50 and 100 μM) or dexamethasone (10-12, 10-10, 10-8 and 10-6 M) followed by treatments with CSE (2.5, 5%), or TNF-α (10 ng/ml), or trichostatin A (100 ng/ml). Here we reported the discovery that baicalin significantly protected pulmonary function and attenuated CS-induced inflammatory response by decreasing inflammatory cells and production of TNF-α, IL-8 and MMP-9, which was poorly achieved by dexamethasone. Baicalin also showed efficacy on enhancing HDAC2 activity and protein expression, however, it did not affect HDAC2 mRNA. Further studies revealed that baicalin inhibited HDAC2 phosphorylation, suggesting it may directly affect the protein structure and effect by
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modification at post-translational level. Together these results suggest that baicalin plays a critical role in modulating HDAC2, leading to restoration of corticosteroid sensitivity, finally attenuating CS-induced respiratory inflammation.
A-227Establishment and comparison of combining disease and syndrome model of asthma with ‘kidney Yang deficiency’ or ‘abnormal Savda’
Li Bei 1, Luo Qingli 1, Mammat Nurahmat 2, Du Yijie 1, Jin Hualiang 1, Wu Xiao 1, Lv Yubao 1, Sun Jing 1, Dong Jingcheng 1*1 Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, 200040 China; 2 Xinjiang Uighur Medical
Training College, Wada, Xinjiang, 848000 China; *Corresponding author: Tel/fax: +86 21 52888301. E-mail address:
[email protected] (J. Dong).
Objective: Firstly, our study aims to establish and evaluate rat models of kidney Yang deficiency syndrome (KYDS) and abnormal Savda syndrome (ASS). General state, body weight, food and water intake, behaviors, function of hypothalamic-pituitary-target organ axes (HPTOA) and sympathetic/parasympathetic (S/P) nerve system were observed and compared between the two rat models. Secondly, the study aims to establish and evaluate rat models of combining disease and syndrome model of asthma with KYDS or ASS. General state, body weight, food and water intake, behaviors, function of HPTOA and S/P nerve system, airway hyperresponsiveness (AHR), airway inflammation, and airway remodeling were recorded and compared between the two rat models. Methods: The‘KYDS’model was induced by high dose of exogenous corticostrone by multi-point subcutaneous injection; the‘ASS’model was induced by multiple factors including reared in a dry and cold environment, given dry and cold food, experienced chronic intermittent plantar electric shock, forced swimming and fixed brake; Then, the above mentioned rat models were respectively sensitized and challenged by ovalbumin (OVA) and models combining disease and syndrome of asthma with‘KYDS’or‘ASS’were established. Sixty male SD rats were randomly divided into six groups: normal control group (NC), asthma control group (AC), kidney Yang deficiency syndrome group (KYDS), abnormal Savda syndrome group (ASS), kidney Yang deficiency syndrome –asthma group (KYDSA), and abnormal Savda syndrome-asthma group (ASSA). General state, body weight, food and water intake were recorded everyday; the open field test (OFT) and sucrose preference test (SPT) were used to assess changes of behavioral indicators; the weight of thymus, spleen, adrenal, thyroid and testicular were measured and organ index of those glands were calculated; Enzyme-linked immunosorbent assay (ELISA) method was used to observe the level of corticostrone (CORT), adrenocorticotropic hormone (ACTH), 3,5, 3′-triiodothyronine (T3), thyroxine (T4), thyroid-stimulating hormone (TSH), estradiol (E2), testosterone (T), luteinizing hormone (LH), follicle stimulating hormone (FSH), cyclic adenosine monophosphate (c AMP) and cyclic guanosine monophosphate (c GMP) in plasma in order to assess the function of HPTOA and S/P nerve system; Buxco's new FinePointeTM RC system was used to detect airway resistance (RI) and AHR in response to methacholine (Mch); Bio-Plex suspension array technology was applied to detect the level of cytokines such like interleukin (IL)-2, IL-5, IL-6, IL-10, IL-13, granulocyte-macrophage colony stimulating factor (GM-CSF) and interferon-γ (IFN-γ) in bronchoalveolar lavage fluid (BALF) and serum; Hematoxylin-eosin (H&E) staining was used to evaluate perivascular eosinophilia, peribronchiolar eosinophilia, epithelial damage and oedema in the
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lungs of rats; Periodic Acid-Schiff (PAS) staining was utilized to assess mucus secretion; Masson's staining was applied to assay the amount of collagen deposition. Results: Compared to the NC group, body weight of the other five groups were significantly reduced after modeling (P<0.01), and the ASS group was the most obvious group in body weight loss. Moreover, body weight was slightly restored in the groups of combining disease and syndrome during the process of allergy exposure (P<0.05), but there were still significant differences with the NC group (P<0.05). The weight loss of ASSA group was more serious than that of KYDSA group (P<0.05); Food and water intake were markedly increased in the ASS with or without allergy exposure groups, but decreased in the KYDS with or without allergy exposure groups compared to the NC group (P<0.01). Moreover, food and water intake in the AC group were less than the NC group (P<0.01); The results of OFT demonstrated that the counts of total cross, horizontal activity and vertical activity were markedly diminished in the KYDS, KYDSA, ASS and ASSA groups in contrast to the NC group (P<0.01). However, the activities included fecal boli in OFT were significantly increased in the AC group (P<0.01); The results from SPT showed that preference for sucrose was sharply declined in the groups of KYDS with or without allergy exposure and ASS with or without allergy exposure (P<0.01), in addition, the ASS group was the most obvious group; The organ index showed that OVA-exposed induced thymus and spleen hyperplasia implied immune-activated (P<0.01), and thymus in the rats of KYDS and ASS groups were atrophy indicated immune-suppressed (P<0.05, P<0.01); the KYDS with or without allergy exposure group manifested as adrenal and thyroid atrophy (P<0.05), but testicular hyperplasia (P<0.05). Above three glands were hyperplasia in the ASS with or without allergy exposure group (P<0.05). The adrenal of AC group was atrophy (P<0.05), but there was no significant difference in thyroid and testicular; ELISA results indicated that the levels of CORT, T4, TSH, LH, FSH notably increased in plasma in the groups of KYDS with or without allergy exposure group (P<0.01), but the level of ACTH significantly decreased (P<0.05) compared to the NC group, which demonstrated those groups had the dysfunction of HPTOA. In contrast to the NC group, the levels of hormones such as CORT, ACTH, T4, TSH, LH, FSH were sharply increased in the ASS with or without allergy exposure (P<0.01), which indicated activating HPTOA. Although the level of CORT dropped down and ACTH dropped up in the AC group, there was no significant difference with the NC group. The level of c AMP and the ratio between c AMP and c GMP remarkably reduced in the other five groups compared with the NC group (P<0.01), which indicated that there was the dysfunction of S/P nerve system in those groups; The results of invasive pulmonary function test illustrated that OVA-challenged rats developed AHR compared to those without allergy exposure, typically reflected by high RI in response to increasing concentrations of Mch (P<0.05， P<0.01). RI was higher in the KYDSA group than that in the AC group (P<0.05), but was lower than that in the ASSA group at the Mch concentrations of 12.5mg/ml and 25mg/ml. Moreover, the change of RI from baseline was significantly increased in the ASSA group compared to the AC group at every concentrations of Mch (P<0.05); Bio-Plex method showed that OVA-exposed sharply increased the levels of Th2 inflammatory cytokines like IL-6, IL-13 in BALF and serum (P<0.01), but Th1 cytokines like IL-2, IL-10 was markedly decreased (P<0.01) compared to those without allergy exposure, which illustrated that OVA-exposed induced the imbalance of helper T cells 1(Th1)/Th2. Though linear correlation analysis, changes of some cytokines in serum were consistent with those in BALF; Lung histopathological studies showed that inflammatory cells especially eosinophils and lymphocytes infiltration in the peribronchial and perivascular areas (H&E staining), mucus overproduction and goblet cell hyperplasia (PAS staining) and emerge of collagen deposition (Masson staining) were observed in the OVA-exposed groups (P<0.01). Moreover, the degree of inflammatory cells infiltration in the peribronchial and perivascular areas
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(P<0.05) and emerge of collagen deposition (P<0.05) were more serious in the KYDSA group compared with the AC group. The scores of inflammatory cells infiltration (P<0.05) and collagen deposition were higher in the ASSA group compared to the AC group. Conclusion: On the one hand, usage of the high dose of corticosterone or multiple factors could successfully establish the KYDS or ASS rat model, and the two models had similar changes in biological characterization, abnormal behaviors, dysfunction of HPTOA and S/P nerve system, but varied in different degree. On the other hand, based on above rat models and then combined with ovalbumin exposure could successfully set up models of combining disease and syndrome of asthma with KYDS or ASS, moreover, those models had either the characteristic of pathology of asthma such as AHR, airway inflammation, airway remodeling, which were more serious than allergy exposure alone, or the syndrome performance of Kidney-Yang deficiency in traditional Chinese medicine (TCM) and Abnormal Savda in traditional Uighur medicine (TUM), which indicated the feasibility and reliability of those models.
A-228Icariin attenuates LPS-induced acute inflammatory responses: Involvement of PI3K/Akt and NF-κB signaling pathway
Xu Changqing 1, Liu Baojun 1, Wu Jinfeng 1, Xu Yanchun 2, Duan Xiaohong1, Cao Yuxue 1, Dong Jingcheng 1*1 Lab of Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, Shanghai, 200040, China; 2 Department of
Physiology and Pharmacology, West Virginia University, WV, 26506, USA; *Corresponding author: Tel.: +86 21 52888301,
Fax: +86 21 52888265, E-mail address：[email protected]
This study aimed to investigate the mechanism underlying the attenuation of LPS-induced lung inflammation by icariin in vivo and in vitro. The anti-inflammatory effects of icariin on LPS-induced acute inflammatory and the molecular mechanism were investigated. Pretreatment with icarrin (20 mg/kg) could attenuate acute lung inflammation by inhibiting mRNA expressions of tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), metalloproteinase cycloxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS) in the lung of LPS-treated mice. In addition, icariin suppressed the secretion of TNF-α, prostaglandin E2(PGE2) and nitric oxide (NO) as well as NF-κB p65 activation. Furthermore, decreased myeloperoxidase(MPO) activity was observed in the lung tissue and LPS-induced cytotoxicity in the RAW 264.7 macrophages cells was also markedly attenuated by icariin. Western blotting analysis and confocal microscopy showed that icariin pretreatment reduced the nucleus transportation and constant level of NF-κB p65 in the RAW 264.7 macrophage cells. However, the protective effects of icariin were reversed by a PI3K/Akt inhibitor(wortmannin). Our in vitro and in vivo results suggested that activation of the PI3K/Akt pathway and the inhibition of NF-κB were involved in the protective effects of icariin on LPS-induced acute inflammatory responses.
A-229Icariin inhibits corticosterone-induced apoptosis in hypothalamic neurons via the PI3-K/Akt signaling pathway
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Zhang Hongying 1, Liu Baojun 1, Wu Jinfeng 1, Xu Changqing 1, Tao Jiang 2, Duan Xiaohong 1, Cao Yuxue 1, Dong Jingcheng 1*1Laboratory of Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, 200040, Shanghai, China; 2Department
of General Dentistry, Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of
Stomatology, 200011, Shanghai, China; *To whom correspondence should be addressed (Full postal address :Laboratory of
Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, Shanghai, China 200040.Telephone +86-21-52888301;
Fax +86-21-52888265; E-mail: [email protected])
Excessive corticosterone is known to induce neuronal damage in many regions of the brain，particularly the hippocampus, which is the mainly area implicated in depression. However, little research has been done on alterations to hypothalamic neurons in depression and the cellular and molecular basis for the changes. In the present study, we aim to determine whether corticosterone causes apoptosis in primary cultured hypothalamic neurons and to investigate the protective effects of icariin, an active natural ingredient from the Chinese plant, Epimedium sagittatum maxim. Our study shows that exposure of hypothalamic neurons to corticosterone causes a significant loss in viability, a significant decrease in mitochondrial membrane potential (MMP), an increase in caspase-3 activity ,an elevation in intracellular reactive oxygen species (ROS) elevation and decreased superoxide dismutase (SOD) activity . However, pretreatment of the cells with icariin prior to corticosterone exposure was founded noticeably suppress these corticosterone -induced events. noticeably. Furthermore, icariin can prevent corticosterone-induced cell death via activation of PI3/Akt pathway. In conclusion, icariin can prevent corticosterone-induced hypothalamic cell apoptosis via activation of the PI3/Akt pathway.
A-230Pharmacokinetics of forsythoside after intravenous administration in beagle dogs
Shi Rong 1, Xuan Zhenyu 2, Ma Yueming 1, L.Yingying 1, L.Hongguo 3, S.Tianjiang 3
1Laboratory of Pharmacokinetics, Shanghai University of Traditional Chinese Medicine, Shanghai, China; 2Shanghai Youseen
New Medicine Exploitation Co, Ltd, China Shanghai, China; 3Yangtze River Pharmacy Group, Taizhou, China
A high-performance liquid chromatography with ultraviolet detection method was established and validated for quantification of forsythoside concentrations in dog plasma. Following a single-step protein precipitation with perchloric acid, the forsythoside and internal standard were separated on a reversed-phase C18 column with water-glacial acetic acid-methanol as mobile phase at a flow rate of 1 mL/min with ultraviolet detection at 326 and 278 nm for forsythoside and IS, respectively. The calibration curve for forsythoside was linear over a range of 0.052-13.33 ug/ml, with correlation coefficient of 0.999. The within- and between-batch precisions of analysis were <8 % and accuracy was 95-107%. After intravenous administration of forsythoside at the doses of 5, 10, and 20 mg/kg, the Cmax values for forsythoside were estimated to be of 12.33, 22.90 and 54.45 ug/mL, respectively. The AUC increased with the increasing of doses, and the mean AUC0-t values were 5.69, 11.80, and 18.66 mg·h/L, respectively. Forsythoside was eliminated quickly and the mean T1/2 values at doses of 5, 10, and 20 mg/kg were 1.36, 1.49, and 0.71h, respectively. The pharmacokinetics of forsythoside in beagle dogs complied with linear kinetic course in the dose range.
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Research on the correlation between expression of foxp3 protein and airway inflammation and the effects of Icariin in a mouse model of asthma
Jin Hualiang 1, Li Bei1, Luo Qingli 1
1Lab of Integrative Medicine for Lung，Inflammation and Cancer，Huashan Hospital，Fudan University，Shanghai 200040,
China
Objective: To investigate the role of FOXP3 protein expression within the CD4+CD25+Foxp3+Treg cells in the pathogenesis of asthm in a mouse model, and explore the effects of icarrin on the airway inflammation and expression of FOXP3. Methods: 32 Balb/c mice were randomly divided into 4 groups, i.e., the normal control group, the model group, the dexamethasone group, the icariin group, with 8 mice in each group. Asthma model was established by ovalbumin sensitization and aerosol challenge in all groups except the control group. The mice of each treatment group were administrated with dexamethasone and icariin respectively by gavage, while mice in the normal control group were administrated with physiological saline. Within 24 houres after the last ovalbumin challenge, airway reactivity was measured by the invasive measurement system. Changs in airway histopathology were observed. Polychromatic flow cytometry was applied to delineate the CD4+CD25+Foxp3+Treg cells in spleen. The level of FOXP3 protein expression in lung tissue was assessed by Westem blot. IL-10 in BALF and serum were measured by ELISA. Results: Compared with the control group, the airway hyperresponsiveness and airway inflammation was significantly aggravated in model group (P < 0.05). The percentage of Treg cells was similar between the control and model groups (P < 0.05). The average expression of FOXP3 protein within Treg cells in spleen and lung tissue was significantly decreased (P < 0.05). In BALF and serum of the asthma group, the level of IL-10 was signifivantly lower (P < 0.05). Dexamethasone treatment significantly attenuated airway hyperresponsiveness and inflammation, and increased the average expression of FOXP3 protein within Treg cells in spleen and total FOXP3 protein in lung tissues, and the IL-10 levels in BALF and serum were increased ; Icariin also significantly attenuated airway hyperresponsiveness and inflammation, and increased the expression of total FOXP3 protein in lung tissues and IL-10 levels in serum, and had a tendency to increase the average FOXP3 expression within Treg cells in spleen and IL-10 level in BALF. Conclusion: In a mouse model of asthma, the expression of FOXP3 protein is decreased within their CD4+CD25+Foxp3+Treg. Our findings also suggests that icariin may increase the FOXP3 expression in lung tissue and the level of IL-10, which may contribute to its anti-inflammatory effects.
A-232Some Chinese Medicine Components Attenuating Oxidative Stress and Airway Remodeling in COPD Rat via Keap1-Nrf2-ARE Signaling Pathway
Lv Yubao1, Li Lulu 1, Sun Jing 1, Gong Weiyi 1, Jin Hualiang 1, Luo Qingli 1, Du Yijie 1, Li Bei 1, Wang Genfa 1, Dong Jingcheng 1*1Department of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China
*Corresponding author: Tel.: +86 21 52888301, Fax: +86 21 52888265, E-mail address：[email protected]
Objective: Investigating how Icariin, Astragaloside ,Catalpol, Baicalin and their compounds to reduce oxidative stress and airway remodeling in COPD rat. Methods: (1) SD rats were grouped and modeled
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as same as part 1. (2).To observe the level of Nrf2 expression in the lung with immunohistoc-hemistry. (3) Taking the right lung tissue to extract mRNA and observing the expression of Keap1, Nrf2, CAT, GCLC, HO-1, SOD, NQO1 mRNA with Real-time-PCR. (4) Evaluation of Nrf2, GCLC, CAT, HO-1, NQO1 and Keap1 protein.exp-ression in lung tissue by Western blotting. Results: (1) Compared with the control group, the immunohistochemical positive expression of Nrf2 in model group was significantly higher (P <0.05), while Nrf2 expression in the Dexamethasone group was not statistically significant compared with the model group (P> 0.05). Compared with the model group, the levels of Nrf2 expresssion in the NAC group, the Icariin group, the Astragaloside group, the Catalpol group, the Baicalin group and the compound groups rised with the doses increased. the difference was significant (P<0.05). (2) Real-Time-PCR showed that the expression of Nrf2 mRNA in the model group was significantly higher than that of the normal control group (P<0.05); the expression levels of CAT, GCLC, HO-1, SOD, and NQO1 mRNA tended to increase, but there is no significant difference (P>0.05); Keap1 mRNA expression tended to decrease, but it was not statistically significant (P>0.05). Compared with the model group, the expression of Keap1, Nrf2, CAT, GCLC, HO-1, SOD and NQO1 mRNA in the Dexamethasone group was not statistically significant (P>0.05). The expression levels of Nrf2,CAT,GCLC, HO-1,SOD, NQO1 mRNA in the NAC group , the Icariin group, the Astragaloside group, the Catalpol group, the Baicalin group and the compound groups were enhanced with the dose of drugs increased, and at the highest dose, the differences were significant (P<0.05). However, the keap1 mRNA expression went down with the increasing of the dose of drugs, at the highest dose, the expression level was significantly lower than that of the model group, the difference was significant (P<0.05). (3) Nrf2 protein expression was significantly higher in the model group than the normal control group(P<0.5), the expression levels of CAT, GCLC, HO-1 and NQO1 protein tended to increase, but there was no significant difference (P>0.05), Keap1 protein expression decreased slighly, but it was not statistically significant (P>0.05). The tendence of Keap1, Nrf2, CAT, GCLC, HO-1 and NQO1 protein expression in the Dexamethasone group was consistent with the model group, it was not statistically significant (P>0.05). Compared with the model group, the expression levels of Nrf2, CAT, GCLC, HO-1, NQO1 protein in the NAC group, the Icariin group, the Astragaloside group, the Catalpol group, the Baicalin group and the compound groups were increased with the dose of drug raised, the difference was statistically significant (P<0.05); Keap1 protein expression decreased with the dose of drugs increased, at the highest dose, the difference was statistically significant (P<0.05). Conclusion: 1) NAC, Icariin, Astragaloside, Catalpol, Baicalin and their compound formulas could promote Nrf2 protein expression and nuclear translocation in the lung of COPD rat induced by cigarette smoke; 2) NAC, Icariin, Astragaloside, Catalpol, Baicalin and their compound formulas increased Nrf2 protein and Nrf2 mRNA expression, strengthening with the increasing of the dose; 3) NAC, Icariin, Astragaloside, Catalpol, Baicalin and their compound formulas could down-regulate Keap1 protein and mRNA expression in the lung of COPD rat induced by cigarette smoke. 4) NAC, Icariin, Astragaloside, Catalpol, Baicalin and their compound formulas could activate Nrf2 downstream pathway, and increase antioxidant enzymes and detoxifying enzymes in‖phase expression in protein and mRNA level, strengthening with the dose rised; 5) Icariin, Astragaloside, catalpol, baicalin and their compound formulas could alleviate oxidative stress damage and airway remodeling in COPD rats induced by cigarette smoke, which might be associated with the activation of Keap1- Nrf2- ARE signaling pathway.
A-233The Effects of Icariin, Baicalin on Keap1-Nrf2-ARE Signaling Pathway and Beas-2B Cells
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Induced by Cigarette Smoke Extract (CSE)
Lv Yubao 1, Li Lulu 1, Sun Jing 1, Gong Weiyi 1, Jin Hualiang 1, Luo Qingli 1, Du Yijie 1, Li Bei 1, Wang Genfa 1, Dong Jingcheng 1*1Department of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China
*Corresponding author: Tel.: +86 21 52888301, Fax: +86 21 52888265, E-mail address：[email protected]
Objective: This study was to investigate the effect of Icariin & Baicalin on oxidative stress and Keap1-Nrf2-ARE signaling pathway in human bronchial epithelial cells (Beas-2B) induced by CSE, and explore their active mechanism. Methods: (1) Cultured bronchial epithelial cells were divided into eight groups: Group A: normal control group; Group B: model group stimulated by tobacco extract (CSE); Group C: pretreating low-dose (20mmol/L) Icariin for 24h then stimulating CSE for 4h; Group D: pretreating middle-dose (40mmol/L) Icariin for 24h then stimulating CSE for 4h; Group E: pretreating high-dose (80mmol/L) Icariin for 24h then stimulating CSE for 4h; Group F: pretreating low-dose (20mol/L) Baicalin for 24h then stimulating CSE for 4h; Group G: pretreating middle-dose (40mol/L) Baicalin for 24h then stimulating CSE for 4h; Group H: pretreating high-dose (80mol/L) Baicalin for 24h then stimulating CSE for 4h. (2) Cell morphology was observed under an inverted microscope. (3) Cell viability was assayed by CCK-8. (4) The Concentrations of ROS, IL-8, IL-10, GSH, CAT, SOD and HO-1 in the supernatant of Beas-2B cell were measured by biochemical methods. (5) The expression levels of Nrf2、Keap1 mRNA in Beas-2B were detected by Real -time PCR. (6) The expression levels of Nrf2, Keap1, NQO1, GCS, HO-1 protein were detected by Western blotting. Results: (1) 10% of CSE induced oxidative stress injury and death of Beas-2B cell,cell viability considerably decreased compared with the normal group, the concentrations of ROS, IL-8 in the supernatant were considerably increased (P<0.05), the concentrations of IL-10, GSH, CAT, SOD, HO-1 levels went down significantly (P<0.05). (2) Compared with the model group, Icariin & Baicalin significantly reduced CSE-induced Beas-2b cell death and inhibited the reduction of cell viability. However, after pretreating Icariin & Baicalin, the concentrations of ROS & IL-8 in the supernatant were on the decrease with the dose increased, while the concentrations of IL-10, GSH, CAT, SOD, and HO-1 were elevated with the dose rising, when the concentrations of Icariin & Baicalin were greater than 40mol/L. they all showed a significant statistical difference at medium or high dose(P<0.05). (3) Compared with the normal group, the expression of Nrf2 mRNA increased in the Beas-2B cell induced by 10% CSE, while Keap1 mRNA expression decreased, that is a statistical significance (P<0.05). Compared with the model group, Icariin & Baicalin significantly increased the expression of Nrf2 mRNA in the Beas-2B cell induced by CSE and reduced Keap1 mRNA expression in a concentration-dependent manner. (4) Compared with normal group, the expression of Nrf2 mRNA increased in the Beas-2B cell induced by 10% CSE, while Keap1 mRNA expression decreased, that is a statistical significance (P<0.05).Compared with the model group, Icariin & Baicalin obviously increased the expression of Nrf2 mRNA in the Beas-2B cell induced by CSE and reduced Keap1 mRNA expression in a concentration-dependent manner. (5) Compared with the normal group, the expression of Nrf2, GCS, NQO1, HO-1 proteins increased in the Beas-2B cell induced by 10% CSE, while Keap1 protein expression substantially decreased (P<0.05). Compared with the model group, Icariin & Baicalin considerably increased the expression of Nrf2, GCS, NQO1, HO-1 protein in the Beas-2B cell induced by CSE and reduced Keap1 protein expression in a concentration-dependent manner. Conclusion: (1) 10% CSE could induceBeas-2B cells oxidative stress damage and death. (2) Icariin & Baicalin can clearly inhibit CSE-induced Beas-2B cell death and improve the cell viability. (3) Icariin &
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Baicalin inhibited the generation of endogenous oxide, with increasing of GSH, HO-1, SOD and IL-10. (4) Icariin & Baicalin activated Nrf2 downstream pathway and increased the antioxidant enzymes and‖phase detoxifying enzymes in protein and mRNA level in a concentration-dependent manner. (5) Icariin & Baicalin reduced oxidative stress injury in the CSE induced Beas-2B cell, which might associate with activation of the Keap1-Nrf2-ARE signaling pathway.
A-234The Effects of some Chinese Medicine Components on Oxidative Stress and Airway Remodeling of COPD Rats
Lv Yubao 1, Li Lulu 1, Sun Jing 1, Gong Weiyi 1, Jin Hualiang 1, Luo Qingli 1, Du Yijie 1, Li Bei 1, Wang Genfa 1, Dong Jingcheng 1*1Department of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China
*Corresponding author: Tel.: +86 21 52888301, Fax: +86 21 52888265, E-mail address：[email protected]
Objective: By studying the effects of Icariin, Astragaloside, Catalpol, Baicalin and their compounds for SD rats with chronic obstructive pulmonary disease, and the changes of lung histopathology, pulmonary function and cytokines, we will evaluate the action of these Chinese medicine components & their compounds for COPD rats’ oxidative stress & airway remodeling, explore the efficacy mechanism, and expect to provide a theoretical basis of the clinical application about Chinese medicines for COPD. Methods: (1) 220 male SD rats were randomly divided into 22 groups (n = 10). Group A: normal control group; Group B: COPD model group; Group C: COPD model + NAC group (800mg/(kg)); Group D: COPD model + Dexamethasone group(0.1mg/(kg.d)); Group E: COPD model + low-dosage-Icariin group(10mg/(kg.d)); Group F: COPD model+ middle-doseage-Icariin group(20mg/(kg.d)); Group G: COPD model +high- doseage- Icariin group(40mg/(kg.d)); Group H: COPD model + low-dosage -Astragaloside group(4mg/(kg.d)); Group I: COPD model+middle-doseage -Astragaloside group (8mg/(kg.d)); Group J: COPD model +high-doseage -Astragaloside group(16mg/(kg.d)); Group K: COPD model + low-dosage -Catalpol group(10mg/(kg.d)); Group L: COPD model+middle-dosage-Catalpol group(20mg/(kg.d)); Group M: COPD model+high-dosage-Catalpol group(40mg/(kg.d)); Group N: COPD model+low-doseage-Baicalin group(20mg/the (kg.d)); Group O: COPD model+ middle-dosage-Baicalin group(40mg/(kg.d)); Group P: COPD model+high-doseage-Baicalin group(80mg/(kg.d)); Group Q: COPDmodel+low-dosage-Astragaloside+low-dosage-Icariin+low- dosage-Catalpol; Group R: COPD model+middle-dosage-Astragaloside+middle-dosage-Icariin +middle-dosage-Catalpol; Group S: COPD models+high-dosage-Astragaloside+high-dosage-Icariin+ high-dosage-Catalpol; Group T: COPD model+low-dosage-Astragaloside+low-dosage-Icariin+low-dosage-Catalpol+low-dosage-Baicalin; Group U: COPD model+middle-dosage-Astragaloside+middle-dosage-Icariin+middle-dosage- Catalpol+middle-dosage-B aicalin; Group V: COPD models+high-dosage-Astragaloside+high-dosage- Icariin+high-dosage-Catalpol+high-dosage-Baicalin. (2) COPD animal model was established by cigarette smoke exposure. (3) The rat lung function was checked by the Buxco spirometer. (4) The concentrations of IL-8, IL-10, TNF-α, TGF-β1 and ET-1 in BALF were measured by enzyme-linked immunosorbent assay. (5) The concentrations of IL-8, IL-10, TNF-α, TGF-β1, ET-1, MMP-1, MMP-9 and TIMP-1 in serum were measured by enzyme-linked immunosorbent assay. (6) Using biochemical methods to detect the concentrations of GSH, SOD, CAT, HO-1, and MDA in the lung tissue homogenates; (7) Tissue was fixed by formalin from right lung. Sagittal sections were
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stained with hematoxylin-eosin, and observed for the bronchus and lung histopathological changes. (8) The altering of inflammatory cell infiltration under bronchial mucosa, the number of fibroblasts, the thickness of bronchial smooth muscle in 200m, the thickness of smooth muscle in small pulmonary arteries in 200m, the mean pulmonary artery wall area, and the average alveolar area were observed with pathological image analysis instrument. Results: (1) COPD model was made successfully in SD rat. Lung function test showed that FEV0.1/FVC (%), PEF, and MMEF were significantly lower in the group with COPD than the normal control group (P <0.05); FRC of the COPD group was significantly higher than that of the normal control group (P <0.05). In the Dexamethasone group, there was no significant improvement in lung function and no statistical significance (P>0.05), compared with the model group.Lung function in the NAC group, the Icariin group, the Astragaloside group, the Catalpol group, the Baicalin group and the compounds , was significantly improved compared with the model group, and it enhanced with the increasing of the dose(P <0.05). (2) HE staining pathological section of lung tissue in model group showed alveolar septa destruction, enlargement of alveolai, infiltration of alveolar macrophages and neutrophils, and increasing of goblet cell. Lung tissue inflammation grade, the number of fibroblasts, thickness of bronchial smooth muscle, thickness of pulmonary artery smooth muscle, mean pulmonary artery wall area, and the average alveolar area in model group were significantly higher than the normal group (P<0.01). Compared with the model group, the pathological ex-tent of the damage in the Dexamethasone group was not improved markedly (P>0.05). However, the pathological changes lessened gradually after interventio-n of NAC, Icariin, Astragaloside, Catalpol, Baicalin and compounds, as well as the proliferation & the degree of inflammation, and they became near to nor-mal while the dose rised gradually. (3) Compared with the normal group, the levels of serum IL-8, TNF-α, TGF-β1, ET-1, MMP-1, MMP-9, TIMP-1 in the COPD model group were significantly increased (P<0.05 or P<0.01), but suppression inflammatory cytokine IL-10 reduced obviously (P <0.05). Compared with the model group, the levels of serum IL-8, TNF-α, MMP-9, TGF-β1 in Dexamethasone group were not significantly different (P>0.05), but the levels of serum MMP-1, TIMP-1, and ET-1 were clearly lower than that of the model group (P <0.05), and IL-10 rised significantly(P <0.05). Compared with the model group, the serum levels of IL-8, TNF-α, TGF-β1, ET-1, MMP-1, MMP-9, TIMP-1 in the NAC group, the Icariin group, the Astragaloside group, the Catalpol group, the Baicalin group and the compound groups gradually reduced with the dose incresed, serum IL-10 rised with the dose increased. At the highest doses, they all were statistically significant(P<0.05 or P<0.01). (4) Compared with the normal group, the levels of IL-8, TNF-α, TGF-β1 and ET-1 in BALF in the model group were significantly increased, while the IL-10 decreased obviously(P <0.05 or P <0.01). Compared with the model group, the levels of IL-8 and ET-1 in BALF significantly decreased in the Dexamethasone group (P <0.05), but TNF-α and TGF-β1 did not go down significantly (P> 0.05), and IL-10 rised significantly(P <0.05). Compared with the model group, the levels of IL-8, TNF-α, TGF-β1 and ET-1 of BALF in the NACgroup, the Icariin group, the Astragaloside group, the Catalpol group, the Baicalin group and the compound groups gradually reduced with the dose incresed, but IL-10 went up with the dose added. At the highest doses, they all were statistically significant (P<0.05 or P<0.01). (5) Compared with the normal group, the MDA level of lung homogenate in the model group was significantly increased (P <0.05), and the level of SOD, CAT, HO-1 significantly reduced (P <0.05), while the level of GSH decreased slightly (P> 0.05). Compared with the model group, the MDA level of lung homogenate in the dexamethasone group significantly decreased (P<0.05), however, the levels of CAT, GSH, SOD and HO-1 in lung homogenate were not significantly improved (P> 0.05). Compared with the model group, the MDA level of lung homogenates in the NAC group, the Icariin group, the Astragaloside
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group, the Catalpol group, the Baicalin group and compound groups were significantly decreased, while the levels of SOD, CAT and GSH, HO-1 rised significantly. At the highest dose, they were statistically significant (P<0.05). Conclusion: 1) After six months of passive smoking, we successfully copied the rat COPD airway remodeling model. 2) COPD model group rats manifested severe oxidative/antioxidant imbalance. 3) Icariin, Astragaloside, Catalpol, Baicalin and their compounds characterized with anti-inflammatory and antioxidant capacity, and could improve lung function and airway remodeling in COPD rat. When given medium or high dose of Icariin, Astragaloside, Catalpol, Baicalin for COPD rat, their efficacys were equal to or better than the NAC. 4) NAC had a strong anti-inflammatory and antioxidant role, and could effectively improve the airway remodeling. Dexamethasone’ efficacy in COPD was not obvious, and long-term use of hormones would accompany with a lot of side effects.
A-235The research on the relationship between compatibility of Buzhongyiqi Decoction and immune regulation
Luo Qingli 1, Dong Jingcheng 1, Guo Xin 2*, Yang Yang2
1Affiliated Huashan Hospital, Fudan University, 200040, Shanghai, China;2 Shanghai University of Traditional Chinese
Medicine , 201203, Shanghai, China: *Correspondence should be addressed to E-mail: [email protected]
Objective: To analyze the different compatibility value and the regulation of immune effect of Buzhongyiqi Decoction. Method: CTX was injected into rats’ abdomen to make rat model of poor immunity. We chose spleen index, thymus index, splenic lymphocyte proliferation test and Quantitative Hemolysis Spectrophotometry to detect the medical effects among Buzhongyiqi Decoction, each herbal medicine in this decoction and immune regulation. Result: Both Decoction and each herbal medicine in the decoction have the functions of strengthening the non-specificity, cell and humor immunity of poorly immuned rats in different levels（P<0.05，P<0.01）. Conclusion: Different compatibility value and doses could have different effects on immune pesticide effect.
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A-236A comparison of the serum and sputum inflammatory mediator profiles in asthma and COPD
Cao Yuxue #, Gong Weiyi #, Zhang Hongying, Liu Baojun, Li Bei, Wu Xiao, Duan Xiaohong *, Dong Jingcheng *Institute of Integrated Traditional Chinese and Western Medicine, Department of Integrated Traditional Chinese and Western
Medicine, Huashan Hospital, Fudan University. 12 Middle Wulumuqi Road, Shanghai, P. R. China. #Contributed equally,
*Correspondence: Professor Xiaohong Duan (E-mail: [email protected], Fax: 86-21-52888265) and Professor Jingcheng Dong
(E-mail: [email protected], Fax: 86-21-52888265).
Objective: Cytokines play a vital role in orchestrating the inflammation in asthma and COPD. This study aimed to investigate the serum and sputum cytokine pattern in asthma and COPD patients. Methods: Clinical characteristics, blood and sputum in asthma, COPD subjects and healthy controls were collected. Blood and sputum processing was performed for serum and sputum supernatant respectively. Cytokines were measured by enzyme-linked immunosorbent assay (ELISA) and inflammatory antibody array. Results: Assess by ELISA, only a few cytokines expression increased in COPD group compared to healthy subjects, while measured by antibody array more mediators showed differentiation. In the analysis of sub-phenotypes, cytokines differential expression were more significant between eosinophilic and non-eosinophilic airway inflammation than between asthma and COPD, showed both in the results measured by ELISA and antibody array. However, little difference was found in subgroups between smoking and non-smoking subjects. Conclusions: Both conventional differentiation, asthma and COPD, and the novel taxonomy, eosinophilic and non-essinophilic airway inflammation may reveal the differential expression of inflammatory mediators, making clinicians understand the cytokines network of airway inflammation better.
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A-237A new type health food supplement with analgesic and Anti-inflammatory Activities, ard-p401, but without adverse reactions
Michio Katagi1，Akio Yasukawa2，Chiaki Kudoh3，Yasuhiro Komatsu4，Eiichi Hujihira5
1Kawagoe Animal Medical Center, Saitama, Japan; 2Nishiogi Animal Hospital, Tokyo, Japan; 3Kudo Chiaki Hospital, Japan; 4Sun R and D Institute for Natural Medicines Co.Inc.Ginza, Japan; 5College of Hokkaido Pharmaceutical Science, Hokkaido,
Japan
In Japan, around 25% of the population is over 60 year old people. In aged people, osteoarthritis and dislocation of the backbone cartilage induce defect of the locomotive activity and reduction in Quality of Life (QOL). The non steroidal anti inflammatory drugs (NSAIDs) has been taking for a therapeutic treatment of the diseases. NSAIDs, however, induce the well known adverse reactions such as peptic gastric ulcer. Therefore, we need safer medicines that are allowed to use for long-term therapy without any adverse reactions in digestive organs. is uncertain even their approved safety. Then, development of a new type of a health food supplement that consists of foods and produce anti-inflammatory and analgesic activities would be requested. The authors have already developed a new health food supplement, SRD- P001 (Sun R&D Institute for Natural Medicines Inc. Ltd. in Tokyo) which contains ginger, western white willow, cinnamon, bitter orange, glucosamine and so on. These plants (crude drugs) show anti inflammatory, analgesic effects, and anti oxidative activity. In this time, we would like to present the efficacy of SRD-P401 (a modified SRD-P001) that is a newly developed health food supplement with anti CoxⅠ and CoxⅡ, sedative and analgesic, anti-inflammatory and, anti-oxidant action, on dogs and/or cats with joint diseases. In order to know the clinical efficacy (pain control, anti inflammatory activity) of SRD-P401, it was assessed by giving to patient dogs and cats with intervertebral spondylopathy, osteoarthritis, postoperative pain control. SRD-401 exhibited anti pain and anti inflammatory activities with no side effects. These results indicated that it could be a useful health food supplement in the veterinary medicine.
A-238A study of 406 cases with fever in the year between March 2009 and March 2010
Li Zheng 1, Zhang Yanxin 1, Li Fengsen1*1Affiliated Traditional Chinese Medicine Hospital Xinjiang Medical University, 830000, Urumqi, Xinjiang Uighur Autonomous
Region China; *Correspondence should be addressed to [email protected]
Objective: To investigate the etiology, diagnostic methods and procedure for management with fever diseases of the respiratory system. Methods: All 406 cases of respiratory disease with fever admitted to the respiratory department of Chinese Traditional Medical Hospital of Xinjiang between March 2009 and March 2010.Medical records were reviewed retrospectively. Results: There were 225 male patients and 181 female patients with an average age of 45. 34 years old. 385 cases had infectious disease with 183 cases of pneumonia; 139 cases of acute upper respiratory tract infection; 27 cases of tuberculous pleuritis; 28 cases of pulmonary tuberculosis; 21 cases of acute treacheobronchitis; 5 cases of lung abscess; 31 cases of acute exacerbations of chronic obstructive pulmonary disease; 21cases of bronchiectasis; 21 of the 406 cases had non-infectious disease with 9 case of lung cancer; 11 cases of
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interstitial pneumonia; 1 cases of extrinsic allergic alveolitis. The fever pathogenesis of tuberculosis is longer then other infectious diseases. the level of fever of tuberculosis is lower then other infectious diseases. there are no difference between infectious disease and non-infectious disease. In these 406 cases, 128 cases (31. 52%) can give final diagnosis by normal clinical data, 221 cases (54. 31%) need CT examination to concluded; there are 9 1cases need pathology examination to identify; other 48 cases (11. 82%) have to diagnose by the result of test cure. Conclusions: Infectious diseases were the major causes of respiratory disease with fever. The common reasons are pneumonia; acute upper respiratory tract infection; tuberculosis; acute exacerbations of chronic obstructive pulmonary disease. Xinjiang has a high incidence rate of Tuberculosis, it should be reconstructed in diagnosis. Common diseases can be diagnosed by the clinical data, CT examination is very important in the diagnosis of the respiratory disease with fever, pathology examination is accurate, and the test of cure is an obbligato method for the diagnosis of Tuberculosis.
A-239Anti-inflammatory and endocrine-regulated effects of icariin on cigarette smoking induced chronic obstructive pulmonary disease in rats
Zeng Lixuan #, Gong Weiyi #, Wu Jinfeng, Zhang Hongying, Liu Baojun, Cao Yuxue, Du Yijie, Duan Xiaohong *, Dong Jingcheng * Institute of Integrated Traditional Chinese and Western Medicine, Department of Integrated Traditional Chinese and Western
Medicine, Huashan Hospital, Fudan University. 12 Middle Wulumuqi Road, Shanghai, P. R. China. #Contributed equally, *Co-
correspondence: Professor Xiaohong Duan (E-mail: [email protected]; Fax: 86-21-52888265), Professor Jingcheng
Dong (E-mail: [email protected]; Fax: 86-21-52888265).
Background: Chronic obstructive pulmonary disease (COPD) is a major health problem worldwide, and herbal medicines have a long history of experience and proven successful treatment for COPD. However, little information is available on the effect of icariin on cigarette smoke (CS) induced COPD. Objectives: To investigate the effects of icariin administration on CS-exposed COPD rats. Methods: COPD rat model was established by CS exposure. Thirty-six rats were randomly assigned to six groups: control, CS, dexamethasone (DXM), and icariin (20 mg/kg, 40 mg/kg, 80 mg/kg). The lung pathology was observed and leukocytes in bronchoalveolar lavage fluid (BALF) were counted by optical microscope. Pulmonary function was measured by using an animal plethysmograph. The levels of cytokines and hormornes were measured both by enzyme linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). The expression of NF-κB p65 protein were detected by immunohistochemistry. Results: Abnormalities of pulmonary function and pathology were observed in CS-induced COPD rats, and CS exposure significantly increased expression of pro-inflammatory mediators in serum and BALF, down-regulated levels of corticotropin releasing hormone (CRH) and corticosterone (CORT) in tissue
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and enhanced NF-κB p65 expression in the lungs. Icariin administration significantly protected pulmonary function and attenuated the CS-induced inflammatory response by reducing inflammatory cells and production of IL-8, IL-6 and TNF-α. In addition, icariin showed endocrine-regulated effect by increasing CRH and CORT expression. These results were not found in rats treated with DEX. Conclusions: Icariin has anti-inflammatory and endocrine-regulated effects in CS-induced COPD rat model and its effectiveness enhances with the increase of dosage.
A-240Anti-inflammatory effects of icariin on cigarette smoke induced inflammatory responses in mice
Li Lulu1, Wu Jinfeng 1, Liu Baojun 1, Sun Jing 1, Gong Weiyi 1, Lv Yubao1, Zhang Hongying 1, Luo Qingli 1, Du Wenjing 1, Xu Hailin 1, Dong Jingcheng *1Department of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China;
*Corresponding author: Tel.: +86 21 52888301; Fax: +86 21 52888265; E-mail address：[email protected]
The present study was conducted to investigate the effects of icariin, a major constituent of flavonoids isolated from the herb Epimedium, on cigarette smoke (CS) induced inflammatory responses in mice. BALB/c mice were exposed to smoke of 15 cigarettes for 1 h/day, 6 days/week for 3 months and dosed with icariin (25, 50 and 100 mg/kg) or dexamethasone (1 mg/kg). We found that icariin significantly protected pulmonary function and attenuated CS-induced inflammatory response by decreasing inflammatory cells and production of TNF-α, IL-8 and MMP-9. Further studies revealed that icariin administration markedly restore CS-reduced GR protein expression, which might subsequently contribute to the attenuation of CS-induced respiratory inflammatory response. Together these results suggest that icariin has anti-inflammatory effects in cigarette smoke induced inflammatory models in mice, possibly achieved by modulating GR protein expression.
A-241Application of the Delphi method of development of chronic obstructive pulmonary disease "fast"、"slow" criterion
Li Fengsen 1,2*, Gao Zhen 2, Jing Jing2, Xu Dan2
1Department of Respiratory ，Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University；2Xinjiang State
Research Center of Traditional Chinese Medicine, Urumqi, 830000, Xinjiang Uighur Autonomous Region ， China ；*Correspondence should be addressed to [email protected]
Objective: Initial application of the Delphi method in patients with chronic obstructive pulmonary disease progression fast, slow to judge basis. Method: Determine the development of chronic obstructive pulmonary disease to determine the speed of evaluation, the main indicators and secondary sub-indicators, the development of expert advice table, using the Delphi method, 50 experts carried out a questionnaire consultation, mainly related to clinical respiratory specialists and related medical professionals, from 12 states Xinjiang, urban areas, with good representation of the subject. Results: Develop a course and grade as the main indicators of lung function, a positive coefficient of 90% of the experts, the development of rapid indicators to determine dyspnea index score ≥3 than the maximum
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76%, slow development of indicators to determine the level of lung function ≤Ⅱ score than the maximum 69 %, concentration of expertise, coordination is good, desirable prediction, two questionnaire survey 24 indicators selected 11 indicators. Conclusion: Delphi method to develop COPD in the initial development of "fast" "slow" to judge based on a certain role, duration, lung function levels, the annual number of hospitalizations, CAT score could be used as disease progression in patients with COPD, "fast"、"slow" basis.
A-242Baicalin is anti-inflammatory in cigarette smoke-induced inflammatory models in vivo and in vitro: A possible role for HDAC2 activity
Li Lulu1, Wu Jinfeng 1, Liu Baojun1, Sun Jing 1, Gong Weiyi 1, Lv Yubao 1, Zhang Hongying 1, Luo Qingli 1, Wu Xiao 1, Dong Jingcheng 1*Department of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, Shanghai 200040, China
*Corresponding author: Fax: +86 21 52888265. E-mail address: [email protected] (J. Dong).
Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory disease characterized by airway obstruction and progressive lung inflammation, which is insensitive to corticosteroids therapies. In this study, we investigated the mechanism underlying the attenuation of cigarette smoke (CS)-induced respiratory inflammation by baicalin, a flavonoid compound isolated from the root of Scutellaria baicalensis Georgi, in vivo and in vitro. In vivo, mice were exposed to smoke of 15 cigarettes for 1 h/day, 6 days/week for 3 months and dosed with baicalin (25, 50 and 100 mg/kg) or dexamethasone (1 mg/kg). In vitro, A549 cells were incubated with baicalin (10, 50 and 100 μM) or dexamethasone (10−12, 10−10, 10−8 and 10−6 M) followed by treatments with cigarette smoke extract (CSE, 2.5 and 5%), or TNF-α (10 ng/ml), or trichostatin A (TSA, 100 ng/ml). We found that baicalin significantly protected pulmonary function and attenuated CS-induced inflammatory response by decreasing inflammatory cells and production of TNF-α, IL-8 and MMP-9. This result was not found in the group treated with dexamethasone. Baicalin also showed efficacy in enhancing histone deacetylase (HDAC)2 activity and protein expression, however, it did not affect HDAC2 mRNA. Further studies revealed that baicalin inhibited HDAC2 phosphorylation, suggesting that it may directly affect the protein structure and effect by modification at post-translational level. Together these results suggest that baicalin has anti-inflammatory effects in cigarette smoke induced inflammatory models in mice and A549 cells, possibly achieved by modulating HDAC2.
A-243Calcineurin/NFAT signaling pathway mediates ET-1-induced proliferation by regulating phosphodiesterase-5 (PDE5) in pulmonary artery smooth muscle cells
Lu Jiamei1, Xie Xinming1, Han Dong1, Li Shaojun1, Li Manxiang1*1Respiratory Diseases Research Center, The Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an
710004, China; Corresponding author: LI Man-xiang, E-mail: [email protected]
Objective: To examine whether Calcineurin/NFAT signaling pathway leads to ET-1-induced proliferation of pulmonary artery smooth muscle cells (PASMCs) by regulating phosphodiesterase-5
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(PDE5) and whether the selective calcineurin inhibitor Cyclosporine A(CsA) or PDE5 inhibitor Sildenafil suppresses ET-1-induced PASMCs proliferation. Methods: Endothelin-1(ET-1) was applied to stimulate PASMCs proliferation with or without prior treatment of cells with the selective calcineurin inhibitor Cyclosporine A(CsA) or PDE5 inhibitor Sildenafil. Calcineurin activity was measured using a calcineurin activity assay kit. PDE5 expression was examined using immunoblotting. cGMP level was detected using a cGMP direct immunoassay Kit. PASMCs proliferation was determined using a [3H]thymidine incorporation assay. Results: ET-1 caused a 2.05-fold increase in the calcineurin activity, a 1.80-fold increase in PDE5 expression, a 3.20-fold increase in the DNA synthesis rate，and reduced the cGMP level by 67%. While CsA reversed ET-1-induced above effects. Inhibition of PDE5 by Sildenafil abolished ET-1-induced reduction of cGMP level. Either CsA or sildenafil suppressed ET-1 stimulated PASMCs proliferation. Conclusion: Activation of calcineurin/NFAT signaling pathway mediates ET-1-induced PASMCs proliferation by stimulating PDE5 expression which further degrades cGMP. The selective calcineurin inhibitor CsA or PDE5 inhibitor sildenafil suppresses ET-1-stimulated PASMCs proliferation.
A-244Catgut implantation at acupoint on the effects of infertility of PCOS rats
Tian Yali 1, Yin Gaizhen 1
1 Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University ， Urumqi, 830000, Xinjiang Uighur
Autonomous Region，China
Objective: observation points to thread burying polycystic ovary syndrome (PCOS) rats reproductive endocrine hormone and ovarian the morphology of the influence, to investigate the PCOS model of the possible role of rats mechanism, to provide the data for clinical research. Methods: 24 days of age female SD rats subcutaneous injection androgen joint human gonadotropins (HCG) made mode for the legal system in the rat model PCOS, the same period control injection saline. PCOS rats randomly divided into model group, ClomiPhene group, embedding + Clomiphene group, embedding samples, among them, the control group and the model group do not make any processing, other each byte is in 50 days of age therapy since 20 days. After treatment, blood collection, using the united method for determination of serum serum level of each index. Experimental animals in the afternoon with the male rat 6 according to 2:1 ratio and cage, in day 18 of death, observing the blastocyst implantation conditions, and gather the left side ovarian, fixed in more than 4% of formaldehyde together, used as HE dyeing under light microscopy tothe ovary influence. Results: The build, build module had significantly T higher than the control group(P<0.05), with a statistical significance. After treatment, the model group, compared to the control group, buried, buried quad line + CC group, CC group weight, serum T, LH, Leptin were significantly reduced(P<0.05), all have significant difference; Each group FSH level difference was not significant (P>0.05), no statistical significance. Conclusion: Meridians buried by adjusting the gonads axis line may, in ovarian local coordination function, reduce the exceptional increasing LH and T level, at the same time, reduce leptin levels, and promote the development and normal follicle ovulation.
A-245Clinical value of serum procalcitonin and C-reactive protein in diagnosis of bacterial infections
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Wei Yanrong1, Yang Huiqing1*
1 Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University, Urumqi, 830000, Xinjiang Uighur Autonomous
Region，China
Objective: To evaluate clinical value of serum procalcitonin and C-reactive protein in diagnosis of bacterial infections. Methods: 48 cases of patients with bacterial infections as the infection group, and 45 cases of non-patients bacterial infection patients as control group, the two groups of serum PCT and CRP results were determined and had a comparative analysis. Results: 1. The infection group serum PCT level was significantly higher than that of the control group (P<0.05): the positive rate of PCT was 93.8% in the infection group, which was higher than that of the control group(8.9%)(P<0.05);2. Serum CRP level in the infection group was significantly than that of the control group(P<0.05).The positive rate of CRP was 35.4% in the infection group, control group(20.0%),but no significant difference between the two groups(P>0.05);3 The sensitivity, specificity, positive predictive value, negative predictive value and diagnostic rate in the PCT diagnostic for serious bacterial infections sensitive diagnostic is higher than that of the CRP, monitoring serum PCT levels can be used as a specific indicator of serious bacterial infection, which has guiding significance for clinical diagnosis and treatment.
A-246Comparison of standard sample preparation methods with real time fluorescence quantitative PCR of Bacteroides thetaiotaomicron in intestinal from db / db type 2 diabetic mellitus mice
Yi Jinyang 1, Wang Ye 1, Zhu Ming 2, Han Xue 1, Yang Zhenzhen 1, Ji Fengcai 3, Zhen Shutao 4, Mao Xinmin 5, Wang Lifeng 6, Ma Xiaoli 7, Li Linlin 1*1Department of Pharmacology, Xinjiang Medical University, Urumqi 830011, China; 2Department of Immunology, Xinjiang
Medical University, Urumqi 830011, China; 3Animal experiment centor, Xinjiang Medical University, Urumqi 830011, China; 4First Affiliated Hospital, Xinjiang Medical University, Urumqi 830011, China; 5College of Traditional Chinese Medical, Xinjiang
Medical University, Urumqi 830011, China; 6Department of Physiology, Xinjiang Medical University, Urumqi 830011, China; 7Department of Analysis and Testing Center ,Xinjiang Medical University, Urumqi 830011, China; *Correspondence should be
addressed to [email protected]
Objective: This study is to compare the enteric bacteroid content by using real-time fluorescent quantitative PCR with two method of standard preparation in the db/db mice. Methods: To collect the intestinal fecal and extract the microbial DNA, and use the V6 District of 16SrRNA Bacteroides thetaiotaomicron primers to do the RT-qPCR and assay the content of intestinal Bacteroides thetaiotaomicron. We selected the ATCC29148 Bacteroides thetaiotaomicron as a standard strain. Results: The number of Bacteroides thetaiotaomicron in type 2 diabetic mellitus mice db / db group and control db/m group by RT-qPCR, respectively, were 5.23×106±2.30×106, 1.52×107±3.23×106;
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5.23×106±2.25×106, 1.45×107±3.39×106. Compared with the control group of db/m, the number of Bacteroides thetaiotaomicron of db /db mice were increased（P<0.05） . The same results were got from the standard bacteria method and normal group high positive method. Conclusion: Standard bacteria method and normal group high positive method for quantitative detection of Bacteroides thetaiotaomicron have no significant difference in mice , so the two kinds of detection methods can be used to detect the level of intestinal flora.
A-247COPD xide measurement and influencing factors
Wang Nana 1, Li Fengsen1*, Bai Wenmei 1, Zhai Yujiang 1
1 Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University Urumqi, 830000, Xinjiang Uighur Autonomous
Region，China；*Correspondence should be addressed to [email protected]
Objective: Study of patients with COPD, asthma, normal human exhaled nitric oxide ( fractional exhaled nitric oxide, FeNO ) determination of mean and its influencing factors. Methods: Selection of patients with COPD, asthma patients, 30 normal subjects were used in Sweden, Niels ( NIOX ) " exhaled nitric oxide measurement system for determination of the FeNO value ". Results: In patients with chronic obstructive pulmonary disease FeNO mean was 24.7 ± 14.5ppb, FeNO of asthmatic patients mean was 63 ± 50.7ppb, FeNO of normal mean was 18.7 ± 6.2ppb; three groups of subjects in weight was not significantly different ( P > 0.05), age, height, body mass index, FEV1, FEV1/FVC were significant difference ( P <0.05 ). Conclusion: Asthma, COPD patients and FeNO values were higher than those of normal subjects, and no significant difference in body weight, three groups of subjects in the age, height, body mass index, FEV1, FEV1/FVC all have differences, but with the FeNO value determination of no linear correlation between the consideration for sample volume due to less, further study.
A-248Correlation analysis on acute exacerbating COPD patients FENO and pulmonary function
Zhai Yujiang 1, Li Fengsen 2*, Xu Qian 1, M.Huan 2, Su Jun 2
1Xinjiang Medical University Post-graduate College; 2Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical
University Urumqi, 830000, Xinjiang Uighur Autonomous Region ， China ； *Correspondence should be addressed to [email protected]
Objective: To make analysis on the acute exacerbating COPD patients FENO and the related factors of pulmonary function. Method: Select the hospitalized 70 AECOPD patients between 48 and 89 years old as the research object to collect the general information (name、gender、age、nationality、height、weight and body mass index), complete the questionnaire of medical history 、 allergic history、smoking history and asthma history, make tests with Sweden NIOX nitric oxide tester, then record the detected pulmonary fractions including FEV1% and FEV1/FVC%. Results: ⑴The average
FENO fraction of 70 AECOPD patients is 26.4 16.64ppb, the minimum fraction is 7.1ppb, and the maximum fraction is 99.6ppb；⑵ The FENO fraction in different age groups has the statistical
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difference(P＜0.05). As people grow older, the FENO fraction is gradually reduced；⑶ The FENO fraction has no statistical difference among different weight index groups(P＞0.05). As the weight index becomes bigger, the FENO fraction is gradually reduced；⑷ The FENO fraction has the statistical difference among the pulmonary patients in different degree(P＜ 0.05). As the patient's pulmonary function becomes declined, the FENO fraction is gradually reduced; ⑸The FENO fraction is negatively related with age(r=-0.111,P=0.047), BMI(r=-0.098,P=0.042) and FEV1/FVC%(r=-0.002,P=0.044). Conclusions: FENO detection plays an important role for the judgment of airway inflammation for COPD patients.
A-249CPPE (Compound of Pomegranate Peel Extraction) Reduce Adipogenesis Through Inhibit Mitotic Clonal Expansion
Wang Lifeng 1, Tao Yicun 1, Zhang Minfang 1, Jiao Yi 1, Luo Xin 1, Li Linlin 2, Mao Xinmin1*, Ren Jun 1
1Pharmacology Department, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; 2Traditional Chinese Medicine
Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should be addressed to
Obesity closely related to diabetes, cardiac diseases, inflammation and their associated disorders. Current treatment of obesity includes various drugs which cause untoward side effects and inflict economic burden. Medicinal plant products have been highlighted as an alternative management of obesity to some extent. Xinjiang is colonized by different ethnic groups, and plant herb, such as: pomegranate. Pomegranate extract bear a lot function: anti-inflammation, anti-proliferation,etc. In this study, data show CPPE had no cyto-toxicity and reduced adipogenesis in 3T3-L1 cell line. CPPE is required at the onset of differentiation for maximal inhibition. It reduced the cell number, especially within 2 days after classic adipogenic induction .Cell cycle analysis shows that cells differentiated with CPPE are severely restrained from entering the S phase of the cell cycle. And also CPPE attenuate C/EBPαDNA binding activity then inhibit PPAR-γand aP2 expression to influence fat accumulation in final stage. In one word, the adipogenic reduction of CPPE might be through inhibition of early Mitotic clonal expansion(MCE) and fat accumulation in final stage .
A-250Different Type Certificate in Patients with Asthma who Body Fluids, and Peripheral Blood of the Change of the Relevant Immune Index
Su Jun 1, Li FengSen 1,2,3*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;2Affilated Traditional
Chinese Medicine Hospital of Xinjiang Medical University；3Xinjiang State Research Center of Traditional Chinese Medicine,
Urumqi, 830000, Xinjiang Uighur Autonomous Region ， China ； *Correspondence should be addressed to [email protected]
Objective : According to the different type certificate in patients with asthma who saliva, sputum, nasal secretions secretory in immunoglobulin A (SIgA) and blood CD4+, CD8+ T lymphocytes, eosinophilic acidic cationic protein (ECP), serum total IgE (T-IgE) discussed were the attack of asthma mechanism,
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In order to further understand the development of childhood asthma mechanism, and also summarizes different card type asthma the changing characteristics of the relevant immune index, for Chinese medicine to asthma on syndrome differentiation and classification provides an objective basis for prevention and cure of bronchial asthma and provide theoretical basis. Methods: Collect different card type 200 patients with asthma (asthma group) and normal 30 cases (normal group), using the method of ELISA sputum, saliva and nasal secretions SIgA of the week outside the venous and ECP, T-IgE testing; The flow cytometric the blood of CD4+, CD8+ T lymphocytes were detected. Results: (1) asthma in the blood of CD4+, CD8+T lymphocytes, CD4+/CD8+ expression compared with normal group are increased, a statistically significant (P<0.05); Different type certificate in patients with asthma who in peripheral blood CD4+, CD8+, CD4+/CD8+ compared to no difference obvious (P>0.05). (2) asthma group of saliva, sputum, nasal secretion of SIgA content are the normal group reduced significantly, and was statistically significant (P<0.05); Different type certificate in patients with asthma who SIgA content found in saliva compared to no significant difference was statistically significant (P>0.05); Different card type between patients with asthma sputum SIgA content in comparison was statistically significant (P<0.05), cold wheezing patients in the sputum SIgA content is higher than the wind wheezing, virtual wheezing sputum, cold bag hot wheezing patients (P<0.05), and the rest between each group there were no significant difference (P>0.05);Every syndrome type between patients with asthma in rhinal secretion SIgA content is statistically significant compared (P< 0.05), cold wheezing patients in rhinal secretion SIgA content are lower than the wind, cold hot sputum wheezing bag wheezing patients (P<0.05); The rest between each group compared to no difference obvious (P>0.05). (3) every syndrome patients with type serum T-IgE, ECP content is not statistically significant (P>0.05). Conclusion: (1)The bronchus asthma patients with acute episode exist in immune function disorder; (2)Asthma patients in acute episode in the peripheral blood immune index CD4+, CD8+T lymphocytes, T-IgE and phlegm, saliva, nasal secretion of SIgA happens disorder, including the upper respiratory tract secretion of immune index SIgA change particularly obvious;(3) According to Chinese medicine diagnosis and classification, cold wheezing type of immune index in patients with asthma who most obvious change. So in asthma in the prevention and control of the immune function of patients with asthma correct balance disorders especially important, to the upper respiratory tract secretion of immune index test related will help understand the serious attack of asthma in traditional Chinese medicine and in traditional Chinese medicine for asthma syndrome type provide objective theoretical basis points.
A-251Discrimination of Type 2 diabetic patients from healthy controls by GC metabonomics method based on their serum fatty acid profiles
Ma Xiaoli1, Tian Lan 1, Li Linlin 2, Meng Lei 1, Wang Ye1, Mao Xinmin3*1Analytical& testing center, college of Pharmacy, Xinjiang Medical University, Urumqi, 830000, Xinjiang Uighur Autonomous
Region ， China;2Pharmacology Department, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region, China; 3Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur
Autonomous Region , China;* Correspondence should be addressed to [email protected]
Objective: To investigate the serum free fatty acid variation in diabetic patients and healthy volunteers in order to explore the application of metabolic profiling analysis in research of diabetes. Methods: By
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adding n-heptadecanoic acid as internal standard, the human serum were methylesterifiled by H2SO4/CH3OH, extracted by n-hexane, FID detector, programmed. Results: 7 kinds of fatty acids can be separated completely, with a good linear from 2~500ug/ml(r>0.999),the average recovery rate of three level was between 76.6%~121.9%.the method can be used to discriminated 31 diabetes patients and 30 healthy volunteer. Conlusions: The results suggested that serum free fatty acid profiles determined by capillary gas chromatography combined with pattern recognition analysis of the data might provide an effective approach to the discrimination of Type 2 diabetic patients from healthy controls. And the applications of pattern recognition methods have improved the sensitivity and specificity greatly. In this study, metabonomics method was employed to the diagnosis of Type 2 diabetes mellitus (DM2) based on serum lipid metabolites.
A-252Distribution and Prevalence of Human Papillomavirus (HPV) in Uygur Women in China: A Population-Based Genotype Study
L.Hua 1, G.Abdixkur, N.Amat 2, H.Yanchun, Z.Suqin, Z.Mutallip, Z.Feng, G.Kurban, T.Ablimit, F.Mijit, G.Nayim, G.Abliz *1The fifth Department of gynecology of the affiliated tumor hospital of Xinjiang Medical University, Xinjiang Urumqi
830011,China; 2Department of Traditional Uygur Institute, Xinjiang Medical University, Xinjiang 830011, China;
*Correspondence should be addressed to [email protected]
Objective: Cervical cancer represents a major health problem in China as well as in other developing countries. Infection with human papillomavirus (HPV) is the main cause of cervical cancer and its precursor lesion, cervical intraepithelial neoplasia (CIN). HLAII DQB1 polymorphisms have been shown to associate with cervical cancer risk, but results varied among different populations. We aimed to investigate the relationships between the HLA-DQB1 allele polymorphisms and the Uygur women’s susceptibility to cervical carcinomas. Methods: Cervical swabs were obtained from 190 cervical cancer patients and 190 healthy controls; HLA-DQB1 genotyping was performed using a PCR-SSP protocol. Results: The frequency of DQB1*03 was significantly lower among cervical cancer patients overall as
compared to controlsOR<1 (2 ＝6.079 P=0.014 OR=0.683), and the frequency of other HLA-DQB1
alleles have no statistical differences between the cervical cancer group and the control group. Conclusion: This is the first report of HLA-DQB1 alleles associated with cervical carcinoma in Uygur women. HLA-DQB1*03 allele may be the protective gene of the Uygur women with cervical cancer in Kashgar and Hotan Region. The role of HLA alleles in the carcinogenesis of cervical carcinomas may play a important role in the gene interference research of cervical cancer.
A-253Effects of baicalin on airway remodeling in asthmatic mice
Sun Jing 1,2, Li Lulu 1,2, Wu Jinfeng 1,2, Liu Baojun 1,2, Gong Weiyi 1,2, Lv Yubao 1,2, Luo Qingli 1,2, Duan Xiaohong 1,2, Dong Jingcheng 1,2*1 Department of Integrative Medicine, Huashan Hospital, Fudan University, Shanghai, China; 2 Institute of Integrated Traditional
Chinese and Western Medicine, Huashan Hospital, Fudan University, Shanghai, China; *Correspondence: Prof. Dr. Jingcheng
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Dong, Department of Integrative Medicine, Huashan Hospital, Fudan University, Middle Urumqi Road 12#, Shanghai, China.
Email: [email protected] Phone: 52888301 Fax: 52888301
Airway remodelling is an important characteristic of asthma, linking inflammation with airway hyperresponsiveness (AHR). Baicalin, a major active compoment, is isolated from Radix Scutellariae. Many studies show that baicalin has anti-inflammatory, anti-bacterial and anti-allergic effects. Here we investigate the influence of baicalin on asthmatic airway remodeling and the mechanism underlining the anti-remodeling effect in vivo. Asthmatic airway remodeling mice model was established by ovalbumin(OVA) exposure. Seventy female BALB/c mice were randomly assigned to seven experimental groups: blank, OVA, hexadecadrol, control and baicalin (25 mg/kg, 50 mg/kg, 100mg/kg) group. Pulmonary function was measured using an whole body plethysmography in conscious and unrestrained mice. The lung pathology was observed and measured. The production of cytokines in bronchoalveolar lavage fluid (BALF) and serum was measured using enzyme-labeled immunosorbent assay(ELISA) kits and the expression levels of transforming growth factor-β1(TGF-β1) and vascular endothelial growth factor(VEGF) were detected by immunohistochemistry. The protein expression levels of TGF-β1、VEGF、 extracellular signal–regulated kinase(ERK) and p21ras were measured using western bloting. The results show OVA exposure significantly increased the expression of interleukin-13 (IL-13) in BALF and serum, and TGF-β1、VEGF、ERK and p21ras expression in the lungs. Baicalin attenuated the effects of OVA significantly. It can be concluded that baicalin has significant anti-remodeling effect on OVA induced asthmatic airway remodeling mice model by decreasing expression of TGF-β1、IL-13、VEGF and inhibiting the activation of the ERK pathway.
A-254Expression of aquaporin 1 increases proliferation and metastasis potential of lung cancer
Wei Xiaobai 1, Dong Jingcheng 1,2*1Department of chinese integrative medicine, Huashan Hospital, Fudan University, Shanghai, China, 2 Shanghai Medical
College of Fudan University, Shanghai 200040, China; 3 Department of Integrated TCM and Western Medicine, Huashan
Hospital Affiliated Fudan University, Shanghai, China *Correspondence: Prof. Jingcheng Dong, Lab of Integrative Medicine for
Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, Shanghai 200040, China. Tel: 86-21-52888301; fax:
86-21-52888265. E-mail address: jcdong2004@126. com
Objective: To explore roles of AQP1 in lung cancer oncogenesis and development, the effects of AQP1 on lung cancer cell proliferation and migration and metastasis were investigated on the LLC and LTEP-A2 cell lines in vitro. Methods: Both LLC and LTEP-A2 Lung cancer cells with different AQP1 expression were used to study cell proliferation and migration potential. cell proliferation and migration potential. Immunofluorescent assay was used to detect AQP1 expression levels in Both LLC and LTEP-A2 cells. Low level expression of AQP1 by different dose AQP1-siRNA, AQP1 levels were quantified by real-time quantitative PCR and western blot analysis. Immunofluorescence, MMP-2、MMP-9、TGF-β、EGFR and further in vitro study including tumor cell migration and invasion, wound healing assays etc were performed. Results: Tumor cell migration and invasion test, wound healing assay showed significantly decreased migration, invasion and wound healing in AQP1 lower-expression cells vs AQP1-neg-transfected group (control tumor cells). Moreover, our results showed that MMPs decresed
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after AQP1-siRNA in two both cell lines. the higher doses of AQP1-siRNA,the lower level of MMP-2/MMP-9 expression.However,AQP1 knockdown did not have a significant effect on the TGF-β and EGFR. Conclusions: AQP1-facilitated lung cancer cell proliferation and migration, possibly through decresing the expression of MMP-2、MMP-9 in part.
A-255Icariin attenuates airway inflammation and eosinophil infiltration by suppressing eotaxin and CCR3+ production in acute and chronic mouse models of allergic asthma
Cai Cui 1, Du Wenjing 2,3, Li Lulu 2,3, Sun Jing 2,3, Lv Yubao 2,3, Du Yijie 2,3, Dong Jingcheng 2,3*1 Hangzhou Red Cross Hospital, Hangzhou 310003, China; 2 Shanghai Medical College of Fudan University, Shanghai
200040, China; 3 Department of Integrated TCM and Western Medicine, Huashan Hospital Affiliated Fudan University,
Shanghai, China
*Correspondence: Prof. Jingcheng Dong, Lab of Integrative Medicine for Lung, Inflammation and Cancers, Huashan Hospital,
Fudan University, Shanghai 200040, China. Tel: 86-21-52888301; fax: 86-21-52888265. E-mail address: jcdong2004@126.
com
Bronchial asthma is characterized by airway inflammation, airway hyperresponsiveness (AHR), and leukocyte infiltration. Icariin is a major flavonoid isolated from the Epimedium koreanum Nakai. In the present study, we investigated whether icariin attenuates airway inflammation and eosinophil infiltration by suppressing eosinophil chemokine and chemokine receptor production in acute and chronic mouse models of allergic asthma. Intraperitoneal treatment with icariin (50mg/kg) could decrease the serum OVA-IgE level, lung inflammatory scores and the expression of inflammatory cytokines (GM-CSF, IFN-γ, IL-5, IL-6 and TNF-α) in asthmatic mice. Icariin also reduced OVA-induced airway hyperresponsiveness and eosinophil infiltration. The expressions of chemokines and chemokine receptors, especially of eotaxin and CCR3, was also down-regulated in asthmatic mice after icariin treatment. These data suggested that icariin could attenuate airway inflammation and eosinophil infiltration through the down-regulation of eosinophilic chemokines and chemokine receptor expression, in particular eotaxin and CCR3 axis were likely to be involved.
A-256Icariin attenuates glucocorticoid insensitivity mediated by repeated psychosocial stress on an ovalbumin-induced murine model of asthma
Li Bei 1, Duan Xiaohong 1, Wu Jinfeng, Liu Baojun, Du Yijie, Luo Qingli, Jin Hualiang, Gong Weiyi, Dong Jingcheng *1Dr. Bei Li and Dr. Xiaohong Duan contributed equally to the present study. * Corresponding author. Tel/fax: +86 21 52888301.
E-mail address: [email protected] (J. Dong). Department of Integrative Medicine, Huashan Hospital, Fudan University,
Middle, Urumqi Road 12#, Shanghai, 200040, China
Evidence shows that psychosocial stress exacerbates asthma, but there is little intervention to alleviate negative effects of psychosocial stress on asthma. Using a murine model combined psychosocial stress with allergic exposure, we investigated the role of icariin in attenuating the negative effects of stress on
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ovalbumin-induced airway inflammation. The results indicated that icariin administered remarkable increased activity in the center of the open field, reversed airway hyperresponsivenesss, reduced inflammatory cytokines infiltration to lung and whole body and also in part recovered glucocorticoid responsiveness. Furthermore, our data also showed that icariin significantly inhibited psychosocial stress-induced increases of corticosterone and markedly increased GR mRNA and protein expression in the lungs of mice exposed to both stress and allergen. Collectively, we speculate that inducing GR modulation might be the potential mechanisms of icariin to facilitate corticosteroid responsiveness of cytokine production and reduce the negative effects of psychosocial stress on ovalbumin-induced airway inflammation.
A-257Icariside II Induces Apoptosis of Melanoma Cells in vivo through the Downregulation of Survival Pathways
Wu Jinfeng 1,2△, Xu Jinhua 2△, E.Eksioglu 3, C.Xianghong 3, Z.Junmin 3, N.Fortenbery 3, W.Sheng 3*, Jing Chengdong1*1 Department of Integrative Medicine, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, 200040, Shanghai, China; 2 Department of Dermatology, Huashan Hospital, Fudan University, 12 Middle Urumqi Road, 200040, Shanghai, China; 3
Department of Immunology, H.Lee.Moffitt Cancer Center & Research Institute, MRC4-east Room 3072C, 12902 Magnolia Dr ,
Tampa, FL, USA, 33612; △ These authors contributed equally to this work, should be considered as co-first author.
* Corresponding authors e-mail addresses: [email protected], [email protected]
Objective: To evaluate the antitumor effects of icariside II (IS), isolated from Herba Epimedii, on in vitro and in vivo models of melanoma and determine its mechanism of apoptosis. Methods: In vitro: Mouse (B16) and human (A375, SK-MEL-5) melanoma cell lines were treated with IS at different concentrations (0-100μM). Cell viability and proliferation was detected by WST-1 assay and with the xCELLigence system, respectively. Apoptosis was measured by the annexin-V/PI flow cytometric assay. Western blot was used to measure cleavaged caspase 3, survivin, P-STAT3, P-ERK and P-AKT. In vivo: B16 and A375 cells were injected subcutaneously into C57BL/6J and BALB/c-nu mice, respectively. After one week, IS solution at (50mg/kg, 100mg/kg) was administered by intraperitoneal injection (i.p) three times for a week. Tumor size was measured with an electronic digital caliper. Results: IS inhibited the proliferation of melanoma cells in a dose- and time-dependent manner. Treatment of A375 cells with IS resulted in an increased number of apoptotic cells mirrored by increases in cleavaged caspase-3 and a decrease in survivin expression. IS significantly inhibited the activation of the JAK-STAT3 and MAPK pathways but promoted an unsustained activation peak of the PI3K-AKT pathway. IS showed potent antitumor activity against melanoma in vivo. Conclusion: IS dramatically inhibited the proliferation of melanoma cells in vivo and in vitro through the regulation of apoptosis. These effects demonstrate the ability of IS to effectively overcome the survival signals of tumor cells which support further preclinical evaluation of IS in cancer as a new potential chemotherapeutic agent.
A-258Idiopathic acute eosinophilic pneumonia, one case of clinical features of analysis
Zhou Linfu 1*, Zong Xiaofu 2
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1First Affiliated Hospital of Nanjing Medical University, Department of Respiratory Medicine, Nanjing, 210029, China; 2People's
Liberation Army 81 Hospital Department of Respiratory Medicine, Nanjing, 210029, China; *Corresponding authors: Zhou
LinFu, Email: [email protected]
Objective: To investigate the clinical characteristics of patients with idiopathic acute eosinophilic pneumonia (IAEP). Methods: We analyzed one case who were treated IAEP patients with clinical manifestations, imaging and pathological features, and review reports about the IAEP the literature. Results: The patient was a 70-year-old female, cough, chest tightness, and intermittent fever, acute course of the disease, severe hypoxemia. The chest CT showed migratory pulmonary infiltrates with a small amount of pleural effusion. Diagnostic thoracentesis showed pleural fluid eosinophils accounted for 28%. Bronchoalveolar lavage fluid eosinophils accounted for 59%. CT-guided percutaneous needle biopsy shows eosinophil lung infiltration. Conclusion: IAEP is a rare disease, misdiagnosed as severe pneumonia, acute lung injury or acute respiratory distress syndrome, glucocorticoid sensitive cured without recurrence, the prognosis is good.
A-259Impact of psychosocial stress on airway inflammation and its mechanism in a murine model of allergic asthma
Li Bei 1, Duan Xiaohong 1, Wu Jinfeng 2, Liu Baojun 2, Luo Qingli 2, Jin Hualiang 3, Du Yijie 3, Zhang Hongying 3, Cao Yuxue 3, Dong Jingcheng *Department of Integrative Medicine, Huashan Hospital, Fudan University, Middle, Urumqi Road 12#, Shanghai, 200040, China
(Bei Li, Xiaohong Duan, Jinfeng Wu, Baojun Liu, Qingli Luo, Hualiang Jin, Yijie Du, Hongying Zhang, Yuxue Cao, Jingcheng
Dong) * Corresponding author. Tel./fax: +86 21 52888301; E-mail address: [email protected] (J. Dong).
Objective: Physicians and scientists have already recognized the fact that psychosocial stress evokes asthma exacerbation, however, the mechanism of how stress gets inside the body is little known. This study was to observe the impact of psychosocial stress on airway inflammation and its mechanism in the ovalbumin-induced asthmatic mice combined with social disruption stress. Methods: Thirty-six male BALB/c mice were randomly divided into control group (no manipulation), asthma group (ovalbumin (OVA)-induced), asthma plus social disruption stress group, and social disruption stress group. The open field video tracking system from Med Associates was used to assess animal behaviors like the time mice spent in the center of the open field test, ambulatory and vertical activity, and fecal boli counts. The invasive pulmonary resistance (RL) and cdyn test system from Buxco was used to detect the pulmonary function. Enzyme-linked immunosorbent assay (ELISA) method was used to observe OVA-Ig E, T-helper type 2 cytokines (IL-4, IL-5, IL-13) and corticostrone in mice serum; T-helper type 2 cytokines (IL-4, IL-5, IL-13, IL-6, TNF-α)in BAL fluid; IL-6 and TNF-αlevels in supernatant of splenocyte cultured in vitro. Hematoxylin-eosin (H&E) staining was used to perivascular eosinophilia, peribronchiolar eosinophilia, epithelial damage and oedema in lung histology. Cell count kit-8 assay (CCK-8) was used to detect cell viability and evaluate the inhibitory effect of corticosterone to splenocyte proliferation induced by LPS. Real time-PCR was used to observe GR mRNA expression in mice pulmonary tissue. Western blotting was used to observe GR protein expression in mice pulmonary tissue. Results: The open field test showed that combined exposure to allergen and repeated stress significantly shortened the time the mice spent in the center of the open field (P<0.01), increased
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ambulatory activity (P<0.01) and the count of fecal boli (P<0.01), but deceased vertical activity (P<0.01). Results from invasive pulmonary function demonstrated that airway hyperresponsiveness was enhanced by psychosocial stress compared with allergy exposure alone. The ELISA results showed that cytokines from serum and BAL fluid was significantly increased (P<0.05). Moreover, the lung histology showed that infiltrated inflammatory cells were significantly increased in both allergy exposure and stress experience mice compared with allergy exposure alone (P<0.05). Interestingly, serum corticosterone was remarkably raised by psychosocial stress (P<0.05). In addition, the inhibitory effect of corticosterone on TNF-γand IL-6 in LPS-stimulated splenocyte cultures in vitro was diminished in the allergy exposure plus stress mice compared to the allergy exposure mice. The CCK-8 test revealed that the inhibition effect of corticostrone on splenocyte proliferation induced by LPS was significantly impaired in the stress mice with or without allergy exposure, while no significant effect was observed in the control and allergy exposure groups. Furthermore, expressions of GR mRNA and GR protein were significantly reduced in the lung tissues of allergic exposure mice combined with social disruption stress (P<0.05). Conclusion: Social disruption stress could promote anxiety behaviors; it could activate the hypothalamic–pituitary–adrenal (HPA), but meanwhile enhance airway hyperresponsiveness and inflammation; and it could also impair glucocorticoid sensitivity and its function in a murine model of asthma. The down-regulation of glucocorticoid receptor induced by social disruption stress was in part associated with glucocorticoid insensitivity, which leaded to the asthma exacerbation or the prolonged inflammation respons
A-260Intestinal bacteria support HIV infection？
Shu Zhanjun 1, Li Fengsen 1, Dilinuer Tuerhong 1, Yang Chengxin 2, Wang Xiuling 1, Halmurat Upur 3*1National Chinese medicine clinical research base in Xinjiang National Chinese medicine clinical research base in Xinjiang,
Urumqi, Xinjiang Autonomous Region 830000, China; 2Xinjiang Uighur Autonomous Region Infectious Disease Hospital,
Urumqi, Xinjiang Autonomous Region 830000, China; 3Xinjiang Medical University, Urumqi, Xinjiang Autonomous Region
830054, China; * Correspondence to: [email protected]
Since the 1950s, researches have gradually realized that the body's bacteria help fight infection by crowding out potential pathogens. In the past decades, scientists have even begun to see our microbiota as thick-and-thin allies. However, the influence of gut bacteria on human immunodeficiency virus (HIV) is largely unraveled. Our review likely sheds light on the previously indistinct role of commensal microbiota in retroviral pathogenesis. The delicate yet critical balance between this enormous bacterial population and GIT is gradually destroyed alone with HIV incursion. The leakage into the systemic circulation of bacterial and by-products, such as lipopolysaccharide (LPS) directly stimulate the innate immune system through Toll-like receptors. As a result, TLR4 activation provokes production of IL-10, which mediates immunological tolerance. So a tangible deduction is that intestinal microbes may have involved in triggerring for replication and transmission of HIV, just like other retroviruses.
A-261Metabonomic classification and detection of small molecule biomarkers of malignant pleural effusions
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Zhou Xianmei1, He Cuicui2, Liu Yumei2, Zhao Yang 1, Zhao Dan 2, Du Yun 2, Zheng Weiyi 3, Li Jianxin 2
1Department of Respiratory Medicine, Affiliated Jiangsu Province Hospital of Traditional Chinese Medicine, Nanjing University
of Traditional Chinese Medicine, Nanjing 210029, China; e-mail: [email protected]; Tel & fax: +86 25-86617141; 2State Key Lab of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University,
Nanjing 210093, China; E-mail address: [email protected]; Tel & fax: +86 25-83686419; 3Innov Pharma, Suite 1004, 10
Xinghou Road, Nanjing High-Tech Zone, Nanjing, 210061, China
Up to date, the most of research has been focused on the big molecules in pleural effusions and diagnosis of malignant ones still remains challenging. In the present study, targeting at the small molecules as potential biomarkers to predict the malignancy of the effusions, the metabolic profiles of 81 clinical pleural effusions (41 malignant effusions with lung cancer and 40 benign ones) were investigated through a NMR-based metabonomic approach. In 1H NMR analysis, total 10 small molecules in the effusions were simultaneously determined. Significantly higher mean values of valine, lactate and alanine and markedly lower levels of acetoacetate, trimethylamine-N-oxide (TMAO), α- and β-glucose were observed in malignant pleural effusions compared with those in benign ones. DFA modeling of NMR spectra subjected to a validation allowed the malignant effusions to be discriminated from benign ones with high accuracy of 95.83% and 96.97% in the training and validation groups, respectively. Currently, the conventionally clinical analyses on chemical constituents in effusions could not provide a reliable prediction of malignancy of the effusions, the present results revealed that the small molecules might serve as useful biomarkers for diagnosis of the effusions and the present NMR based metabonomic approach provided a highly valuable potential to rapidly and sensitively predict the malignancy of the pleural effusions.
A-262Mitochondrial gene mutations in Uygur patients with type 2 diabetes mellitus in Xinjiang
S.Abuduryim 1, Nurmuhammat Amat 2*, Halmurat Upur 2*1 First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China; 2Traditional Uygur Medicine Institute, Xinjiang
Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should be addressed to [email protected] or
Objective: To study the correlation between the 7 SNPs (single nucleotide polymorphisms) mitochondrial DNA tRNALeu (UUR) gene and ND1 gene in Uygur patients with type 2 diabetes in Xinjiang. Method: A case-control study composed of 223 type 2 diabetes patients and 292 sex-matched and ethnically matched control participants were enrolled in the study. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for analyzing the distribution of polymorphism at the -597C/G and -572G/A promoter of the IL-6 gene. Result: The frequency of the 3316 (g→a) mutation and 3394 (t→a) mutations in the diabetes group is 3.13% (7/223) and 0.9% (2/223), respectively, and the frequency the of both the 3316 (g→a) mutation and 3394 (t→a) mutations is 0.48% (1/223) in the healthy controls and there were no significant difference between two groups. The other SNPs were not in both groups. Conclusions: There were no relationship between Mitochondrial DNA 3243 (t→c), 3593 (t→c), 325l (a→g), 3264 (a→g), 3290 (t→c), 3316 (g→a), 3394 (t→c) locus mutation with Uygur patients with type 2 diabetes mellitus in Xinjiang.
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A-263More attention should be paid to mast cells/basophils than to IgE in allergy: proposed procedure for diagnosing allergic disorders
He Shaoheng 1
1Clinical Research Centre, the First Affiliated Hospital of Nanjing Medical University, 300 Guang-zhou Road, Nanjing, 210029,
People’s Republic of China, Tel: 0086 25 83672085; Fax: 0086 25 83672085, Email: [email protected]
For the last several decades, the routine procedure for diagnosis of immediate-type hypersensitivity (allergy) is initially based on clinical history and physical examination; and then confirmed by skin prick test with allergen extracts and allergen-specific IgE (sIgE ) measurement (Hamilton, 2010). This complies with the current definition of allergic diseases：a group of diseases largely driven via IgE-dependent mechanisms (Barnes, 2009). However, substantial disagreement between clinical manifestation (including skin prick tests) and sIgE measurement has been noted. For example, the predictive value of sIgE for food allergy, drug allergy and allergic asthma is relatively poor (Hamilton, 2010), which demonstrates that we are overvalued the importance of sIgE in diagnosing allergic diseases. According to current theory of allergy, allergens, IgE and mast cells/basophils represent the causative factors, the transmitter (or messenger) and the effector cells of allergy, respectively. The main problem here is that we use the transmitter IgE to define a group of diseases, which is certainly not sufficient enough to reflect the nature of the diseases. The most important event in allergic diseases is activation of mast cells/basophils rather than production of IgE, as they are the ones initiating the pathological damage and causing clinical problems of allergic patients. Thus, theoretically anything which can activate substantial number of mast cells/basophils in the body can induce allergic rections. Therefore, allergic diseases should be defined as a group of mast cell or basophil activation provoked non-infection diseases, including IgE mediated, IgG mediated and non-IgE or non-IgG mediated (non-immunological) subgroups. Based upon the proposed definition above, the ideal laboratory examination procedure for allergy should be as follows: Skin prick and skin patch tests (highly suspicious of allergy)allergen-induced basophil activation test (confirmation of allergy) sIgE and sIgG measurement (confirmation of subtype of allergy)final diagnosis: IgE, IgG or non-immunological allergy.
A-264Moving and Breathing training on short-term effect of the stable COPD patients
Li Fengsen 1*, Liao Chunyan 1, Du Lijuan 1
1Affiliated TCM Hospital Xinjiang Medical University, 830000, Urumqi, China & National Clinical Research Base of Traditional
Chinese Medicine, Urumqi, 830000, Xinjiang, China; *Correspondence should be addressed to [email protected]
Objective: To observe the movement and breathing training on short-term effect of the out-patient with stable chronic obstructive pulmonary disease (COPD) patients. Methods: 57 cases out-patient in stable COPD patients got conventional treatment at the same time, given the movement and breathing
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training, and observed parameters (FVC%, FEV1%, mMRC, the CAT, the BODE, symptom score) to evaluate the efficacy of treatment. Results: after 6 weeks of treatment, lung function in patients with FVC% unchanged, after 12 weeks, FVC% improved; the treatment of 6 weeks, 12 weeks in patients with symptom score, FEV 1%, the quality of life scores (CAT) compared with the significant improvement in the rehabilitation movement. Conclusion: Exercise and respiratory training after six weeks out-patient in stable COPD patients with symptoms, lung function, quality of life, BODE began to improve, improved significantly after 12 weeks of treatment.
A-265Neuroprotective effects of icariin on corticosterone-induced apoptosis in primary cultured rat hippocampal neurons
Liu Baojun 1, Zhang Hongying 1, Xu Changqing 1, Yang Guang 2, Tao Jiang 3, Huang Jianhua 1, Wu Jinfeng 1, Duan Xiaohong 1, Cao Yucue 1, Dong Jingcheng 1*1Laboratory of Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, 200040, Shanghai, China; 2Brain
Research Centre, University of British Columbia, Vancouver, V6T 2B5, British Columbia, Canada; 3Department of General
Dentistry, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of
Stomatology, 200011, Shanghai, China. *Corresponding author. Fax: +86 21 52888265. E-mail address:
[email protected] (J. Dong).
Neurons are damaged following prolonged exposure to high concentrations of corticosterone, particularly during chronic inflammatory and immune diseases. One of the main mechanisms underlying neuronal injury is apoptosis. In the present study the neuroprotective effects of icariin, an active natural ingredient from the Chinese plant Epimedium sagittatum maxim against corticosterone-induced apoptosis were examined in primary cultured rat hippocampal neuronal cells. Pre-treatment of neuronal cells with icariin suppressed corticosterone-induced cytotoxicity in a dose-dependent manner. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) nickendlabeling assay (TUNEL) labeling demonstrated that icariin significantly reduced TUNELpositive cell numbers induced by exposure of cultured neurons to corticosterone. Moreover, icariin markedly inhibited corticosterone-induced mitochondrial dysfunction, including improved mitochondrial membrane potential and inhibition of caspase-3 activation. Using western blot analysis, corticosterone activated p38MAPK, extracellular regulated kinase 1/2 (ERK1/2) ,and c-jun N-terminal protein kinase 1(JNK1) ,while icariin blocked p38 MAPK, but not JNK1 or ERK1/2. Pharmacological approaches showed that the activation of p38MAPK plays a critical role in corticosterone-induced mitochondrial dysfunction and apoptosis. Taken together, the present results suggest that the protective effects of icariin on apoptosis in hippocampal neuronal cells are potentially mediated through blockade of p38 MAPK phosphorylation.
A-266Pathogenesis of idiopathic pulmonary fibrosis:from initial apoptosis of epithelial cells to lung remodeling?
Jin Hualiang 1, Dong Jingcheng *1Lab of Integrative Medicine for Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, Shanghai 200040,
China (Jin HL and Dong JC); *Correspondence to: Prof. DONG Jing-cheng, Lab of Integrative Medicine for Lung, Inflammation
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and Cancers, Huashan Hospital, Fudan University, Shanghai 200040, China (Tel: 86-21-52888301. Fax: 86-21-52888265.
Email: [email protected])
Idiopathic pulmonary fibrosis (IPF) is a progressive and often fatal form of interstitial lung disease. Despite extensive efforts in research during recent years, the mechanisms of the disease remain poorly understood. Evidence of an inflammatory mechanism, both supportive and contrary, is briefly reviewed in this paper. However, growing evidence has indicated that the apoptosis of alveolar epithelial cells (AECs) may be the early driving force of progression, with subsequent disrupted integrity of the alveolar-capillary basement membrane leading to an abnormal wound healing pathway. Thus, this paper will focus on outlining a process of pathogenesis of IPF from initial apoptosis of AECs to end lung remodeling.
A-267Preliminary Analysis of the miRNA Expression between Fresh and Old Peripheral Anticoagulant Blood
Zhu Manli 1, Jiao Yi 2, Wang Ye 1, Mao Xinmin 3, Li Linlin 1*1Department of Pharmacology, Xinjiang Medical University, Urumqi, 830011, China; 2Department of Biochemistry and
Molecular Biology, Basic Medical Institute； 3College of Traditional Chinese Medicinal, Xinjiang Medical University, Urumqi,
830011, China; *Correspondence should be addressed to [email protected]
To compare the expression of miRNA（microRNA）between fresh and old peripheral anticoagulant blood. To choose 5 pieces of fresh blood samples and 6 pieces of old blood samples respectively，extract total RNA from the whole blood and measure its purity and concentration，and analyze the microRNA expression using real-time quantitative reverse transcription polymerase chain reaction(real-time RT-PCR). Micro-spectrophotometer test，A260 /280 of total RNA is 1.8~2.0. The comparative number of copies of miRNA RNAU6-2 has no statistical differences between fresh and old peripheral anticoagulant blood. miRNA RNAU6-expressed in fresh and old peripheral anticoagulant blood samples have no difference.
A-268Preliminary Analysis of Transcription Level of FOXOs Genes in Patients’ Feet and Ankles Who Suffer Xinjiang Uygur Congenital Clubfoot
Dong Haojie 1, Lv Faming1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: This thesis discusses Xinjiang Uygur congenital clubfoot FOXO1, FOXO3, FOXO4 genes in ankle muscle tissue of foot and ankle conditions in order to understand their roles in development of malformations of vessels thus provide a theoretical basis for the cause of Xinjiang Uighur congenital clubfoot. Methods: To Collect 30 patients with Uygur congenital clubfoot and 30 cases healthy children. And to collect foot and ankle muscle tissue 200mg by surgery or puncture method and venous blood 5ml. Then to extract tissue RNA and reverse transcription kit for DNA inversion are used. By means Of
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RT-PRC amplification, to research on FOXO1, FOXO3, FOXO4 changes in the level of gene transcription. Results: Compared with the normal group, in patients’ foot and ankle muscle tissue who suffer with congenital clubfoot, FOXO1 gene transcription levels were significantly increased (P<0.05). It has statistical significance. FOXO3 gene transcription levels were significantly increased (P<0.05) .It has statistical significance. FOXO4 has no significant difference in the level of gene transcription (P>0.05).There is no statistical significance. Conclusion: The expression of transcription level of FOXO1, FOXO3 gene may be associated with the Xinjiang Uygur incidence of congenital clubfoot and FOXO1, FOXO3 gene may play an important role in the development of malformations of vessels, resulting in occurrence of congenital clubfoot.
A-269Prethrombotic State is a common manifestation of chronic diseases: A clinical study
T.Aji 1, Shajidan Abudoureyimu2*, Nurmuhammat Amat3, Halmurat Upur3*, R.Aizezi 3, J.Wei 3, A.Umar 4,5, Nicholas Moore3,5
1Cardiovacular Diseases Department,Bortala People Hospital,2First Affilated Hospital of Xinjiang Medical University, Urumqi
830054, Xinjiang Uighur Autonomous Region, PR China;3Traditional Uighur Medicine Department, Xinjiang Medical University,
Urumqi 830054, Xinjiang Uighur Autonomous Region, PR China; 4Department of Pharmacology - Xinjiang Medical University,
830054-Urumqi, Xinjiang, People’s Republic of China; 5Department of Pharmacology - Universite de Bordeaux, F-33076
Bordeaux Cedex, France; *Correspondence should be addressed to [email protected] or [email protected]
Objective: Chronic diseases including cancer, diabetes and cardiovascular diseases are thought to be caused by the complex interplay between multiple genes and environmental factors. Studies have indicated that a prethrombotic state, defined by the imbalance between coagulation and fibrinolysis mechanisms, is associated with an increased tendency for individuals to develop chronic diseases. This study aims to investigate whether a prethrombotic state is a common characteristic of chronic diseases in humans. Methods: This study included 285 adult patients with confirmed cancer, diabetes, hypertension and coronary heart disease. We analyzed CD62p expression in platelets, as well as plasma concentrations of tissue plasminogen activator (t-PA), plasminogen activator inhibitor 1(PAI-1), endothelin (ET-1), and fibrinogen (FIB). We also determined the activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT). Results: The results of the aforementioned indexes were compared with those obtained from a group of 30 healthy subjects who served as controls. The results indicated that the expression of CD62P, plasma PAI-1 and FIB levels of patients were significantly higher as compared with those obtained from healthy subjects. In cancer patients, plasma levels of FIB and ET-1 were markedly higher compared with healthy subjects, while plasma t-PA and APTT were markedly decreased. There were no significant differences in serum ET-1 levels and expression of CD41 between the healthy subjects and the patient with chronic diseases. In addition, PT was significantly shortened in patients with cancer, diabetes and hypertension compared to the healthy subjects. There were no significant changes in APTT, PT and TT among the various disease groups. These data indicate that there is increased platelet activation and thrombin activity but lower fibrinolytic function in patients with chronic diseases, suggesting an involvement of a prethrombotic state in chronic diseases. Conclusion: We conclude that a prethrombotic state may be an important component in the pathogenesis of the chronic diseases.
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A-270Puerarin effect and mechanism of inhibition of airway inflammation and airway remodeling in chronic asthma model
Zhou Linfu 1*, Wang Wenlu 1, Li Hongyan 1, Miao Weiwei 1, Wang Fengfeng 1, He Shaoheng 1, Yin Kaisheng 1
1First Affiliated Hospital of Nanjing Medical University, Department of Respiratory Medicine, Nanjing, 210029, China;
*Corresponding authors: Zhou LinFu, Email: [email protected]
Objective: To observe the puerarin on mice with chronic asthma airway inflammation and airway remodeling, and to explore its possible mechanism. Methods: BALB/c mice 24 were randomly divided into normal control group, asthma group, puerarin group (50 mg/kg) in the budesonide group. Ovalbumin (OVA) sensitized and challenged 12 weeks of modeling. The last challenge, after 24 h, the determination of airway responsiveness, HE staining and PAS staining of airway inflammation, Masson staining and hydroxyproline determination and immunohistochemical observation of lung tissue, ELISA detection of serum IgE and BALF cytokines level flow cytometry of spleen dendritic cells (DC) and CD4+ T cell proliferation level, Western blot of lung tissue, thymic stromal lymphopoietin hormone (TSLP) protein expression. Results: the asthma group airway resistance (RL) than in normal group, puerarin group and budesonide budesonide group than asthma group significantly with lower (all P<0.05), puerarin group and budesonide budesonide group intervention effect similar (P>0.05); asthma of BALF inflammatory cells in the total number and eosinophilic cell ratio was significantly higher than the normal group and puerarin group and the budesonide group than in the asthma group was significantly reduced by both (P< 0.05); asthma group BALF cytokines (IL-4 IL -13 of TGF- β1 and VEGF) and serum total IgE, OVA- sIgE in a more normal group was significantly higher puerarin group and the budesonide group compared with asthmatic mice significantly reduced; Airways of the asthmatic group PAS-positive epithelial cells / bronchus , bronchial Masson staining area / basement membrane perimeter , lung tissue hydroxyproline content , bronchial α-SMA - positive staining area / bronchial basement membrane perimeter, lung tissue PCNA levels than normal significantly increase the puerarin group and the budesonide group than in the asthma group significantly reduced; asthmatic mice is higher than the normal group (P<0.05), the treatment group compared with the asthmatic group was lower (P<0.05); asthma group splenic DC and CD4+ T cell proliferation level than the normal group was significantly higher puerarin group and the budesonide group than in the asthma group were significantly decreased (P<0.05); TSLP expression of asthmatic lung tissue was significantly higher than the normal group and puerarin group and the budesonide group than in the asthma group were significantly (P<0.05). Conclusion: Puerarin can inhibit the chronic asthma model, airway inflammation, airway hyper responsiveness and airway remodeling; this inhibition may be related to inhibition of TSLP- DC -mediated Th2 immune.A-271Regulation of STAT signaling in mouse bone marrow derived dendritic cells by respiratory syncytial virus
Jie Zhijun 1,2, Darrell Dinwiddie 2, Albert Senft 2, S.H kevin 2*1 Department of Respiratory Medicine, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, 200240, China;
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2 Infectious Disease Program, Lovelace Respiratory Research Institute, Albuquerque, NM 87108, USA.; * Corresponding
author at: Infectious Disease Program, Lovelace Respiratory Research Institute, 2425 Ridgecrest Drive, SE, Albuquerque, NM
87108, Tel: 1-505-348-9488; Fax: 1-505-348-8567. Email: [email protected]; Email addresses: [email protected] (Z. Jie),
[email protected](D. Dinwiddie), [email protected] (A. Senft), [email protected] (K. Harrod)
Objective: Dendritic cells (DCs) act as a portal for virus invasion as well as potent antigen-presenting cells (APCs) involved in the antiviral host response. Interferons (IFNs) are produced in response to bacterial and viral infection and activate innate immune to efficiently counteract and remove pathogenic invaders. Respiratory syncytial virus (RSV) could inhibit IFN-mediated signaling pathway in epithelial cells; however, the effects of RSV on IFN signaling in the dendritic cells (DCs) are still unknown. Methods: Mouse bone marrow derived DCs (BMDCs) were mock or infected with RSV at different multiplicity of infection (MOI) for 24h, and then treatment with different cytokines such as interferon-β (IFN-β), IFN-γ or interleukin-10 (IL-10). The RNA expressions of RSV nonstructural protein-1 (NS-1) and NS-2 were detected by RT-PCR. The expressions of Janus family kinase-signal transducer and activator of transcription (JAK/STAT) signaling proteins were assessed by immunoblotting assays. The nuclear localization of specific signaling proteins was determined by immunoflourescence assay. Results: Increasing amounts of NS-1 or NS-2 mRNA expression in BMDCs were observed with infected RSV at increasing MOI, suggesting BMDCs were permissive for viral gene expression. Further examination of the IFN-β signaling cascade showed RSV infection increased the total cellular levels of STAT1 and STAT2 in BMDCs, but impaired the IFN-β-dependent phosphorylation and nuclear localization of STAT1 and STAT2. The inhibitory effects of RSV on STAT1 and STAT2 phosphorylation and translocation were abolished by ultraviolet inactivation. In contrast, RSV did not inhibit the IFN-γ-stimulated STAT1 phosphorylation and nuclear localization. IL-10-stimulated STAT3 phosphorylation was also unaffected by RSV. Conclusions: Despite RSV inhibit STAT protein levels through degradation mechanisms in epithelial cells, these findings demonstrate that RSV also can specifically inhibit the type I interferon response in BMDCs through regulation of STAT1 and STAT2 phosphorylation and nuclear translocation.
A-272Research on expression regulation of relevant cytokines of Th17 in COPD Mouse lung tissue
Wang Jing 1，Li Fengsen1，2*，B.Aishan 1
1 Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University；2Xinjiang State Research Center of Traditional
Chinese Medicine, Urumqi, 830000, Xinjiang Uighur Autonomous Region，China
Objective: Research changes of the chronic obstructive pulmonary disease (COPD) mouse Th17 cell. And preliminary study the relationship between Th17 cells with COPD pathogenesis. Methods: The mouse model of COPD was established through the application of smoke continued for 6 months, to collect lung tissue and peripheral blood, measured by ELISA in serum IL-6, IL-17 and IL-10 changes in the level, using Real-Time PCR to detect lung tissue STAT-3 of IL-17 and RORγt mRNA changes in levels. Results: The COPD model group serum IL-17 and IL-6 content was significantly higher than the normal control group (P <0.05), while the expression of IL-10 model group for the control group was
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significantly higher (P <0.05) ; with COPD mouse lung tissue STAT-3, IL-17 and RORγt mRNA level was obviously higher than the control group(P <0.05). Conclusion: Th17 cell may play an important role in the pathogenesis of COPD. And it may induce the development of pulmonary inflammation.
A-273Seeking best menu of walking exercise for a healthy volunteers through the regulation of peripheral white blood cells in number
Nobuo Yamaguchi1, Delixat Yimit 2, Nurmuhammat Amat 2, Parida Wuxur 2, Xue Jie2
1Kanazawa Medical University, Kanazawa, Japan; 2 Traditional Uighur Medicine Department of Xinjiang Medical University, 393
Medical University Road, 830011, PR China
Efficacy and impact of walking exercise has been widely recognized. However, the majority of walkers did not have a scientific background to know the best exercise menu for the one’ QOL; quality of life. The purpose of this study was to demonstrate the best menu of walking that regulates the peripheral white blood cells in number and function as a marker of QOL expression. Healthy male and female subjects who gave informed consent (mean: 41, n=10) were used. Each subject received two types of exercise treatments at intervals of one month or more. The volunteer direct to walk within one hour for 4 km, indicating 4 mets of exercise. On the other hand, the same volunteer asked to clear within the 30 min for the same course; 8km/he, 8mets. The blood samples were collected before and 24 hrs after the exercise, analyzing by conventional procedures and measured the total leukocytes number, ratio of granulocytes and lymphocytes. The adrenalin contents were also measured. Relative changes in total cell counts, ratios of lymphocytes and granulocytes different in the two groups. The regression lines also represented different tendencies. These results showed that 4 mets walking showed the strong regulative effect -0.6893 but 8met walking -0.3618 of lymphocyte, far behind the 4met therapy in CAM as an individualized therapy.
A-274Significance and Enlightment of the Discovery on the Blood, Circulation Driven by Respiration and of the Study on the Mechanism of the Day-Sweat and the Night-Sweat
Li Zhigang1
1 Yu Lin Middile Agricultural School, 71900, Yulin Shaanxi, China
The significance of the discovery on the blood’s circulation driven by respiration is that by the discovery people can know how the respiration effects on the function of the heart and the blood vessel, furthermore can guide their clinical practices better. The significance of the study is that the cause of the mechanism of the day-sweat and the night-sweat is still unknown, people can explore the mechanism of the day-sweat and the night-sweat, thus to know how the day-sweat and the night-sweat effect on human body and what remedy is the most effective for them. The enlightment of the discovery and the study is by them people can know that the scientifical experiment is not the only method for finding out something.
A-275
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The apigenin raised CD4 + CD25 + Treg cells and inhibit the study of the role of airway inflammation in acute asthma model
Zhou Linfu 1*, Li Hongyan 1, Wang Wenlu 1, Wang Fengfeng1, Miao Weiwei 1, He Shaoheng1, Yin kaisheng 1 1First Affiliated Hospital of Nanjing Medical University, Department of Respiratory Medicine, Nanjing, 210029, China;
*Corresponding authors: Zhou LinFu, Email: [email protected]
Objective: To observe the celery factors for acute asthma mouse model of airway inflammation, airway hyper responsiveness and CD4+CD25+ regulatory T cells (Treg) affect. Methods: 30 SPF grade female BALB/c mice were randomly divided into control group, asthma group, removal of CD25 apigenin group, apigenin group (20mg/kg) and the budesonide group. Ovalbumin (OVA) sensitization and excitation modeling. The last challenge after 24 h, the determination of airway responsiveness , HE staining and PAS staining of airway inflammation and mucus secretion , ELISA assay of serum IgE and BALF cytokines by flow cytometry spleen Treg level. Results: the asthma group airway resistance (RL) than in normal group , celery hormone group and the budesonide budesonide group RL than the asthma group significantly with lower , removal of CD25 celery hormone group than the celery prime group significantly with the increase (both P<0.05) ; asthma of BALF inflammatory cells in the total number and eosinophilic cell ratio was significantly higher than the normal group , apigenin group and the budesonide group than asthma group was significantly lower , remove a significant increase in CD25 of apigenin group compared with the apigenin group (all P <0.05). asthma group in BALF of Th2 (IL-4 , IL-13), serum total IgE and OVA-sIgE were significantly higher than the normal group , the apigenin group , the budesonide group than in the asthma group were significantly lower , removal of CD25 apigenin group compared with the celery factors were significantly higher (P<0.05); Asthma group in BALF of Th1 (IFN-γ) cytokines than the normal group was significantly lower , celery hormone group , budesonide budesonide group compared with asthma group increased significantly , removal of CD25 celery prime group celery prime group significantly with lower (all P<0.05) ; asthma group spleen Treg levels than in normal group was significantly reduced apigenin group, spleen Treg levels in the budesonide group compared with the asthma group were significantly increased, removal of CD25 apigenin group than in the apigenin group decreased significantly (P<0.05). Conclusion: Apigenin can inhibit acute experimental asthma, airway inflammation and airway hyperresponsiveness, this inhibition may increase Treg levels and Th2 immune response into immunosuppression.
A-276The influence of different electroacupuncture strength between inflammatory cells factor and cerebral edema in cerebral ischemia reperfusion rats
Duan Lijun 1, Li Heping 1
1 The First Affilated Hospital, Xinjiang Medical University, Urumqi 830011, Xinjiang Uighur Autonomous Region，China Objective: To study the effects of inflammatory cytokines and brain edema in rats of acute cerebral ischemia and reperfusion with different electroacupuncture stimulation intensity. Neurological deficit score (NSS) and the infarct area were observed in each group. The levels of inflammatory cytokines, TNF-α, IL-1β, IL-10 and the content of tissue water in brain in the peripheral blood serum were
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analysed. Explore the therapeutic effect of acupuncture strength of the best EA on rats of cerebral ischemia and reperfusion. Methods: 8 week-old SD rats were randomly divided into 3 groups: the normal group, 10; the sham operation group, 10; model group, 30. Established the middle cerebral artery occlusion (MCAO) and reperfusion model in the model group, then divided its into three groups: the model control group, the weak EA group, the strong electro-acupuncture group. Did the acupuncture 6 hours after the ischemia-reperfusion (I/R). Did the NSS, collected blood of abdominal aortic and the brains after 24 hours. The brain of 2 rats in every intervention group were frozened section, and the infarct area was observed with TTC staining. Meanwhile, the brain water content were detect with wet and dry method. detected the levels of TNF-alpha, IL-1β, IL-10 in the Serum, and the statistical analysis was done for every index. Results: 1. varying degrees nerve function deficit and the right eye Horner's certificate were dicovered in the model group, and the normal group and sham group does not appear above signs, and varying degrees of cerebral infarction were discovered after the TTC staining; 2. Compared with the normal group, the levels of inflammatory cytokines, TNF-α, IL-1β, IL-10 and the content of tissue water in brain were no statistically significant differences (P＞ 0.05) in the sham operation group, and its increase significantly (P＜ 0.01, P＜ 0.05) in the model control group; 3. Compared with the model group, the levels of TNF-alpha, IL-1β decreased significantly (P＜0.05, P＜0.01), the levels of IL-10 and the content of tissue water in brain were no statistically significant differences (P＞0.05) in the weak EA group. But, in the strong EA group, the levels of TNF-alpha, IL-1β and the content of tissue water in brain decreased significantly (P＜0.01, P＜0.05), while, the levels of IL-10 increased significantly (P＜0.01); 4. Compared with the weak EA group, the levels of TNF-alpha, IL-1β decreased significantly (P＜0.05), the levels of IL-10 and the content of tissue water in brain were no statistically significant differences (P＞ 0.05). Conclusion: EA can protect the rats in the acute cerebral ischemia reperfusion by reducing TNF-alpha, IL-1β and brain tissue water content and increasing IL-10 levels of the serum. The efficacy are difference among the different intensity EA, strong stimulation is superior to the weak stimulation.
A-277The Research of Asthma patients with exhaled nitric oxide and blood eosinophil levels
Yang Weijiang 1, Wang Nana 1, Li Fengsen 1，2*
1 Affilated Traditional Chinese Medicine Hospital of Xinjiang Medical University；2Xinjiang State Research Center of Traditional
Chinese Medicine, Urumqi, 830000, Xinjiang Uighur Autonomous Region，China
Objective: to investigate patients with asthma between the exhaled gas of nitric oxide and blood eosinophil percentage level. Methods: 160 cases of patients with acute asthma detect exhaled gas nitric oxide and eosinophil percentage of cell content were collected to study the correlation. Results: patients with acute asthma between the exhaled gas of nitric oxide and blood eosinophil percentage were positively correlated. Conclusion: patients with asthma detection of exhaled nitric oxide and blood eosinophil percentage can improve the diagnostic accuracy.
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A-278The Study of IL-4、IFN-γ Gene Methylation of Allergic Rhinitis in Uygur, Han people
Lou Zhongzhang 1, Gao Li 1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China; *Correspondence should
be addressed to [email protected]
Objective: To investigate the difference of IL-4, IFN-γ gene promoter methylation of allergic rhinitis patients and normal control in Uygur, Han people. Methods: Methylation-specific PCR (MSP) detected IL-4, IFN-γ gene methylation of 50 Uygur cases and 50 Han cases with allergic rhinitis, and 50 normal Uighurs and 50 normal Han cases. Results: The comparison of IL-4 gene methylation rate between the Han nationality AR patients with normal control group is statistically significant (P<0.05). The comparison of IL-4 gene methylation rate between the Han and Uygur in normal control group is statistically significant (P<0.05). The comparison of IL-4 gene methylation rate between the Han and Uygur AR patients is not statistically significant (P>0.05). The comparison of IL-4 gene methylation rate between Uygur AR patients and normal control group is not statistically significant (P>0.05). The comparison of IFN-γ gene methylation rate between the Han and Uygur AR patients, between patients with AR and normal controls have no statistical significance (P>0.05). But the comparison of IFN-γ gene methylation rate between the Han nationality AR patients with normal control group is statistically significant (P<0.05). Conclusion: Artemisia and pigweed are two important aeroallergens of Uygur and Han patients with allergic rhinitis. There is no significant difference in expression of IL-4 gene methylation between the Han and Uygur AR patients. The IL-4 gene methylation rate of the Han patient with AR is higher than the normal Han person, which indicates that IL-4 gene methylation may play an important role in the pathogenesis of allergic rhinitis. The IL-4 gene hemimethylated of the Han nationality group is higher than that of the Uygur nationality group. The IL-4 gene methylation in the Han nationality group may tend to be the positive expression, the Han population susceptible to allergic rhinitis. There is no significant difference in expression of IFN-γ gene methylation between the Han and Uygur AR patients. The role of IFN-γ gene low-methylation in allergic rhinitis needs further research.
A-279The Study on the relationship between Cruor-fibrinolysis system and the HuiZhuoTanZu Syndrome of Coronary Heart Disease
Xie Xiaoliu1, An Dongqing1*1Traditional Chinese Medicine Institute, Xinjiang Medical University, Urumqi 830011, Xinjiang, China;* Correspondence should
be addressed to [email protected]
Objective: The aim of this study is to investigate the plasma level of Fibrinopeptide A (FPA) and Thrombin Activatable Fibrinolysis Inhibitor (TAFI) so as to explore the relationship between CHD Hui ZhuoTanzhu Syndrome and endothelium dysfunction, provide the microcosmic objective basis for working out the diagnose standard for the coronary heart disease Hui ZhuoTanZu Syndrome, in order to make its diagnose tend being externalization. Methods: A total of 170 samples were obtained from the Medical University, including 88 cases of CHD huizhuotanzu syndrome and 82 cases of CHD non-huizhuotanzu syndrome, 80 cases of normal controls, then observe and analyses the general data and the plasma level of FPA､TAFI among three groups. Results:1) Comparison of plasma FPA:Compared
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with normal controls, the lever of FPA of CHD HuiZhuoTanzhu Syndrome group and non- HuiZhuoTanzhu Syndrome group are significantly higher, the difference is statistically significant (P<0.01) ;between CHD HuiZhuoTanzhu Syndrome group and the non- HuiZhuoTanzhu Syndrome group, there is a significant difference in the lever of FPA (P<0.05). 2) Plasma TAFI level in comparison:the lever of TAFI in CHD HuiZhuoTanzhu Syndrome group and non-HuiZhuoTanzhu Syndrome group are higher than healthy control group, the difference is significant (P<0.01), and the lever of TAFI in CHD HuiZhuoTanzhu Syndrome group is higher than non-HuiZhuoTanzhu Syndrome group (P<0. 05). Conclusion: The Pathological basis of CHD HuiZhuoTanZu syndrome is related with the blood high pour-point state, there are more anomalies in Blood coagulation-fibrinolysis system function between the CHD HuiZhuoTanZu syndrome and non-HuiZhuoTanZu syndrome. The increasing contents of FPA ､ TAFI can help us to differentiate the CHD HuiZhuoTanZu syndrome and non-HuiZhuoTanZu syndrome.
A-280Unique performance like pleural effusion fatal huge mediastinal liposarcoma a case
Zhou Linfu 1*, Li Meimei1, Zhang Shijiang2, Shao Yongfeng 2, Huang Mao 1, He Shaoheng 1, Yin Kaisheng 1
1First Affiliated Hospital of Nanjing Medical University, Department of Respiratory Medicine, Nanjing, 210029, China; 2First
Affiliated Hospital of Nanjing Medical Universit, Cardiothoracic Surgery, Nanjing, 210029, China; *Corresponding authors: Zhou
LinFu, Email: [email protected]
A female patient, aged 38, farmer, was admitted to hospital because of progressive dyspnea February. Occasional dry cough, fever, night sweats, chest pain, haemoptysis, paroxysmal nocturnal dyspnea. Chest radiography and chest CT were outside the hospital reports right pleural effusion (CT value of Hu). The thoracentesis anchor outside the hospital is very low, only see 110 mL reddish pleural effusion, pleural effusion biochemicals show exudate. A history of schizophrenia, a history of ectopic pregnancy surgery. Physical examination: T: 36.5 ℃, P: 86 times / min, and R: 18 times / min, and BP: 110 /70 mmHg in Clear consciousness, spirit wilt, orthopnea, and lips cyanosis, tracheal left superficial lymph nodes not palpable swelling. Full right side of the thorax, fremitus enhanced percussion real tone, the right lung breath sounds disappear. The left lung breath sounds were significantly reduced no smell and wet and dry rales. Heart, abdomen, limbs, and nervous system examination was normal. Supplementary examination: admission check ESR 34 mm / h; C -reactive protein 14.1 mg / L ; blood gas analysis (FiO2 33%): at rest , of pH 7.43 , PCO2 of 41 mmHg , PO2 70 mmHg in the BE 2.6 mmol / L, SaO2, 94 %; blood, brain natriuretic peptide , liver and kidney function , tumor markers , tuberculosis , rheumatism , autoimmune indicators are normal. Abdominal B ultrasound was normal. Enhanced chest CT scan showed a right pleural liquid density seen a homogeneous mass (CT value of about 25 to 67 Hu), were seen in lipid density (CT value -94 Hu), the right lung is not, mediastinum to the left. Chest MRI showed right pleural space-occupying completely, clear boundary, signals uneven strips separated the right side of a small amount of pleural effusion. Urgent consultation of the hospital, and immediately transferred to the Department of Cardiothoracic Surgery undergoing open resection of the tumor. Surgery Chengshun Li, take out a whole gigantic mass, brittle, size 35 cm x 26 cm in x 12 cm, weighing 4.5 kg. After HE staining and immunohistochemistry show well-differentiated and mucinous mediastinal liposarcoma. After two weeks in patients discharged, follow-up so far no
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recurrence was found. Phosphatidylinositol kinase catalytic subunit (PIK3CA) gene mutations and survival in patients with a decline. Surgical specimens of gene sequencing and TA cloning. No. 10 of the PIK3CA gene in patients with exon 4 mutations, including the 1615, 1627 and 1634 base point mutation, and 1658 bp frameshift mutation. Kinase spectroscopy confirmed that the NF - κB and Src mucinous liposarcoma is the more active of the two signaling pathways. Combined with gene sequencing and the kinase spectral data, and will undoubtedly promote effective clinical conversion. Clinical intrathoracic liposarcoma is rare, but we have to think about the possibility of a mediastinal liposarcoma in the differential diagnosis of pleural effusion.
A-281Winter Disease consumer Clinical evaluation of asthma paste the treatment of chronic obstructive pulmonary disease
Wu Ruohan1, Bian Yongjun1, Li Guangxi1, Li Hui1, Wang Lei1, Liu Zhiguo1, Liu Shigang1, Li Junling1, Liu Li1, li Guoqin1
1Guang’anmen Hospital affiliated to China Academy of Chinese Medical Sciences, 100053, Beijing, China
Objective: To verify the clinical efficacy of Xiaochuangao Acupoint Paste (XAP) to treat chronic obstructive pulmonary disease (COPD) in the stable phase. Method: A multi center, randomized, double-blind, placebo control study was adopted.142 patients were randomly divided into two groups. Each year the treatment group were treated with XAP while the other group were treated with placebo three times. The the lung function，SGRQ life quality score，BODE index，yearly average times of common cold and acute attack， times of emergency clinics and hospitalization, and so on were observed. Result: Except the total part of SGRQ score, other three parts are lower than the same time in control parts (p<0.05). The same outcome happened in BODE index, the yearly average times of common cold and acute attack (p<0.05). Conclusion: The XAP can significant alleviate the clinic symptoms, improve the quality of life and improve the prognosis of COPD.
A-282Xinjiang Uygur congenital clubfoot genepolymorphism analysis
Dong Haojie 1, Lv Faming 1*1 Affilated Traditional Chinese Medicine Hospital, Xinjiang Medical University, 116，Huanghe Road, Urumqi 830011, Xinjiang
Uygur Autonomous Region, China; *Correspondence should be addressed to [email protected]
Objective: Of Xinjiang Uygur congenital clubfoot FoxO1, FoxO3, FoxO4 genes in ankle muscle tissue of foot and ankle conditions， to understand its role in the development of vascular malformations for Xinjiang Uygur congenital clubfoot and the cause of the disease and provide a theoretical basis. Methods: We collected Uygur congenital clubfoot in 30 patients, healthy children (including Uighur and Han)30 cases, and collected muscle tissue 200mg and blood 5mlby the foot and ankle surgery or puncture method, extraction of tissue RNA, reverse transcription kit for DNA inversion, amplified by RT-PCR of FoxO1, FoxO3, FoxO4 changes in the level of gene transcription study. Results: Compared with the normal group，patients with congenital clubfoot foot and ankle muscle tissue FoxO1 gene transcription levels were significantly increased (p<0.05), there existed significant difference，FoxO3
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gene transcription levels were significantly increased (p<0.05), there existed significant difference，FoxO4 had no significant difference in the level of gene transcription (p>0.05) and was no statistically significant. Conclusion: FoxO1, FoxO3 gene upregulation may be related to the Xinjiang Uygur incidence of congenital clubfoot associated， FoxO1, FoxO3 gene in the foot and ankle vascular malformations may play an important role in the development, resulting in a congenital clubfoot occurrence.
A-283Original article accuracy of 18F-FDG PET/CT for lymph node staging in non-small-cell lung cancers
Liu Baojun1, Dong Jingcheng1*, Xu Changqing1, Z.Chuantao 1, L.JingJing 1, G.Yihui 1, Z.Jun 1, Wu Jinfeng1, Duan Xiaohong1, Cao Yuxue1
1Laboratory of Lung, Inflammation and Cancers, Huashan Hospital, Fudan University, 200040, Shanghai, China; *To whom
correspondence should be addressed (Full postal address :Laboratory of Lung, Inflammation and Cancers, Huashan Hospital,
Fudan University, Shanghai, China 200040.Telephone +86-21-52888301; Fax +86-21-52888265; E-mail:
Background: This retrospective study evaluated the diagnostic accuracy of 2-(F18)-fluoro-2-deoxy-D-glucose-positron emission tomography (18F-FDG-PET)/computed tomography (PET/CT) in the preoperative diagnosis of metastatic mediastinal and hilar lymph node in patients with non-small-cell lung cancer (NSCLC). Methods: A total of 39 patients received preoperative 18F-FDG PET/CT and the postoperative biopsy. We compared preoperative PET/CT scan results with corresponding intraoperative histopathalogic findings in 39 NSCLC patients. The sensitivity, specificity, accuracy, positive and negative predictive value of 18F-FDG PET/CT were assessed. Results: Histopathologic examination confirmed metastasis in 57 out of the 208 excised lymph nodes; 23 of the 57 nodes were mediastinal and hilar lymph nodes. The sensitivity, specificity, accuracy, positive predictive value and negative predictive value of PET/CT in the preoperative diagnosis of mediastinal lymph node metastasis in NSCLC patients were 65%, 96.8%, 92%, 78.5% and 90%, respectively. Conclusions: PET/CT scan showed good accuracy in the preoperative diagnosis of mediastinal and hilar lymph node metastasis in the patients with NSCLC. We recommend that PET/CT scanning be used as a first-line evaluation tool for tumor diagnosis, therapy evaluation and follow-up.
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A-284A new trial for relief-oriented design of molecule from natural ginseng other than hot water extraction
Nobuo YAMAGUCHI1, Tsugia MURAYAMA2, Masafumi NAKATO3, Yasuhiro KOMATSU4
1Kanazawa Medical University, Kanazawa, Japan; 2Hokuriku University, Kanazawa, Japan; 3INMPRC, Kanazawa, Japan; 4INMPRC, Tokyo, Japan
Almost all the Traditional Chinese Medicines (TCM) from plant origin are prepared by hot-water extraction. This type of extraction system had been succeeded more than twenty centennials. However hot water extraction remained almost all the material left as remnant. So it is necessary to recruit a whole materials as effective compound at this modern century． In such a circumstances, we tried to depredate the plant material in order to prepare more neat element form ginseng. We tried to degradate the natural material into piece by mechanically, enzyme mediated and microbial degradation. In order to screening a effective molecule, we set up fundamental and clinical screening system. The first toxic test was nominated by Helsinki declaration for mammals, indicating genetic toxicity for microorganism and mice. The new effects from the materials coming out the enhanced Immmunomodulatory effect, Decrease blood pressure, Anti-diabetic effect, Enhancing sexual potency, Relieve fatigue, Reduce cholesterol levels, formation of the transformed ginsenosides, (Rh2, Rh3 etc.) , easy absorption from the gastro-intestinal tract, production of new metabolites of ginsenosides.
A-285Antibacterial Activities of Extracts and Constituents in the Rhizomes of Diploid and Tetraploid Gingers (Zingiber officinale Roscoe)
A.Patar 1, N.Tanaka 2, H.Maeda 1, K.Ishimaru 2, K.Nishikawa 1, 3, N.Amat *1Faculty of Health and Living Sciences Education, Naruto University of Education,Tokushima, Japan；2 Faculty of Agriculture,
Saga University, Saga, Japan；3 Traditional Uighur Medicine Department, Xinjiang Medical University, 393 Medical University
Road, Urumqi 830011, Xinjiang, PR China；*Corresponding author. Prof Nurmuhammat Amat. Traditional Uighur Medicine
Department, Xinjiang Medical University, 393 Medical University Road, Urumqi 830011, Xinjiang, PR China Tel.: +86 991
4366167; fax: +86 991 4366014 E-mail address: [email protected]
The pungent phenolics were also isolated from the rhizome of tetraploid ginger (Zingiber officinale Roscoe), and their structures were established as [6]-gingerol and [6]-dehydroparadol by chemical and spectroscopic evidence. And extracts and constituents in the rhizomes of diploid and tetraploid gingers were screened for their antibacterial activity against 6 Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Salmonella typhimurium, Salmonella enteritidis, Vibrio parahaemolyticus, Yersinia enterocolitica) and 3 Gram-positive bacteria (Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus). The minimum inhibitory concentrations (MICs) of extracts and constituents in the rhizomes were determined by the microdilution method ranged from 0.49 to 16,000g/ml, and 0.03 to 1,000g/ml, respectively. Extracts and constituents in the rhizomes exhibited a fairly high antibacterial
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activity against the spectrum of all Gram-positive bacteria tested.
A-286Biodiversity: A link between traditional medicine and modern medicine
Anake KIJJOA1
1Instituto de Ciências Biomédicas de Abel Salazar and Centro Interdisciplinar de Investigação Marinha e Ambiental
(CIIMAR),Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
Thai traditional medicines have always been popular as traditional household medications and a long tradition of preparing and using herbs both as food and as medicine is still being practiced in the Thai society. Recently, Thailand has adopted a policy to support Thai Traditional Medicine as an effective and cheaper way to promote good health of the population. Even though a small number of plant species are used as medicinal plants in herbal preparations, many have been popularly claimed to have properties in curing variable diseases. With a vast area of tropical forest and coast lines, Thailand has inexhaustible biodiversity waiting to be exploited. For these reasons, our group has been investigating chemical constituents and their biological activities of the species popularly claimed as having medicinal properties and the related species. Bearing in mind that the tropical soils are also rich of microbial species, we have also investigated the bioactive compounds produced by soil fungi which exhibited synergistic or antagonistic action with plant pathogens. Although there is no record of medicinal uses of marine organisms, the seas of Thailand are considered potential reservoirs of biodiversity and genetic resources. Consequently, we have also investigated the compounds produced by the marine sponges and the sponge-associated fungi which led to isolation and identification of several new compounds with interesting skeletons and biological activities. The bioactive compounds isolated from either plant with traditional use or unclaimed species are potential for further developments of new therapeutic arsenal to combat rare and newly emerging diseases.
A-287Characteristics of traditional acupuncture therapies in Japan, Korea and China
Kenji Kawakita1, Yong-Suk Kim2, L.XiaoPin 3, Matsuo Arai4, Naomi Takazawa5, Nobuo YAMAGUCHI6
1Meiji University of Integrative Medicine, Kyoto Japan; 2Kyung-Hee University, Seoul, Korea; 3Liaoning University of Traditional
Chinese Medicine, Liaoning, China; 4INMPRC, Kanazawa, Japan; 5Takazawa Acupuncture Clinic, Tokyo, Japan; 6Kanazawa
Medical University, Kanazawa, Japan
Efficacy and safety of acupuncture therapies has been widely recognized. The majority of acupuncture practitioners have learned acupuncture in China (C). On the other hand, different acupuncture procedures have also developed in Korea (K) and Japan (J). The purpose of this study was to demonstrate the characteristics of acupuncture procedures performed in three countries. Healthy male subjects who gave informed consent (mean: 21, n=10) were used. Each subject received three treatments at intervals of 2 months or more. The practitioners were asked to make diagnosis and selected best points as individualized therapy for improving health conditions. The blood samples were collected and analyzed by conventional procedures and measured the total leukocytes number, ratio of
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granulocytes and lymphocytes. The adrenalin contents were also measured. Total number of points used in C and K were similar but they were obviously different. In J, shallow acupuncture needling along the channels was done and no name of points was recorded. Relative changes in total cell counts, ratios of lymphocytes and granulocytes differed among the J, C and K. The regression lines also represented different tendencies. These results were not conclusive but we found variation of acupuncture treatments in J, K and C. They have potentials to modulate the immune systems and stress levels in different way. This JCK projects was the first attempt and it offered important information to establish the role of acupuncture therapy in CAM as an individualized therapy.
A-288Current Trends in Medical Education
Muhammad Aslam1
1Shifa College of Medicine, Shifa Tameer-e-Millat University, Sector H – 8/4, Islamabad
A future medical expert [doctor] needs to acquire appropriate scientific foundation [knowledge] for which one has to develop critical thinking and communication; needs to learn clinical skills in order to be professionally competent; needs to develop professional attitude and values for which one has to improve behaviour and collaborations and, needs to be a custodian of population health as a social responsibility for which one has to learn managerial skills and health advocacy. In order to achieve, it is need of the hour to transform teaching to learning, memorizing to understanding and critical reasoning, and examinations to evaluations. We need not to follow criteria but to set standards. The curriculum is required to be student‐centered, patientoriented, integrated, community based, elective driven and systemic. The tomorrow’s curriculum is required to take the pillars of Bloom’s taxonomy into an account [knowledge, comprehension, application, analysis, synthesis and evaluative judgment]. We need to hybridize the component of research in all facets. The content overload and redundancy is required to be shredded and concepts and context [relevance] needs to be merged. Multimodal learning strategies like large group interactive sessions, small group discussion, self‐directed learning, computer‐assisted learning, problem‐based learning, casebased learning, patient‐based learning, performance‐based learning and projects/ assignments are to be introduced and encouraged. The evaluation methodology must be specific, measurable, achievable, realistic and timebound. Efforts be made to transform summative assessment to formative assessment. The formative assessment reforms the process of learning, whereas, summative assessment reframes the outcome. In conclusion, we need to keep abreast with the current trends in curriculum development, learning strategies and accreditation system keeping an account of our regional and cultural delicacies.
A-289Education Management in Faculty of Medicine in Kabul Medical University, Kabul, Afghanistan
Samad Omar1
1 Dean, Faculty of Medicine Kabul Medical University
Faculty of Medicine founded in 1932 and is the first higher education institute in the country, throughout
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years of progress it is promoted to Kabul Medical University which includes 5faculties. The education management is according to law and regulation of Ministry of Higher Education. Selection of students is through an entrance exam. Study in Faculty of Medicine takes 7 years; MD degree is given to the graduates. Each university year is 2 semester and each semester is 16 weeks. Lectures, practical work in laboratory, bedside teaching, PBL, CBL are methods which are used for teaching. Evaluation of students is carried out during the semester and at the end of each semester.
A-290Effectiveness of Integrated Medical Curriculum in Pakistan: A Public Medical College Experience
Muhammad Saeed1*, Muhammad Iqbal Khan2, Zeiyad Afzal Keyani3, Sarmad Lateef Awan4, Syed Sajid Hussain Shah5, Mohsin Shakeel6 1Prof. of Anatomy & Associate Dean; AJK Medical College, Muzaffarabad, Pakistan; 2Prof. of surgery & Dean; AJK Medical
College, Muzaffarabad, Pakistan; 3Assistant Dean Medical Education；AJKMC, Muzaffarabad, Pakistan; 4Assistant Prof. of Anatomy, AJKMC, Muzaffarabad, Pakistan; 5Prof. of Pathology, AJKMC, Muzaffarabad, Pakistan; 6Assistant Prof. of Urology,
AJKMC, Muzaffarabad, Pakistan. *Correspondence: Dr. Muhammad Saeed Shafi, Prof. of Anatomy & Associate Dean AJK
Medical College, Muzaffarabad, Pakistan; E-mail: [email protected]; Fax # +92-5822-920523; Ph. +92-5822-920448;
Cell: +92-333-4603013.
Background: Integration of medical curricula is still a growing concept in Pakistan. Rapidly changing healthcare system (Healthcare teams) and patient centered care has compelled Medical Regulatory Bodies for a paradigm shift in medical curricula1-2. Keeping abreast with the global trends and watching changed local needs, Azad Jammu & Kashmir Medical College (AJKMC) was the 1st public sector medical college in Pakistan to adopt an outcome based integrated undergraduate curriculum since its inception in 20113. To contextualize the training of future physicians and make Basic Health Sciences (BHS) instruction more relevant to the clinical practice; we introduced integrated, system based Modular spiral curriculum at trans-disciplinary level. Study Design: An action research involving mix-method approach with a dominant qualitative focus. Action research is a participatory research which involves the researchers and participants in an iterative loop of analysis and planning an intervention; evaluation of its impact; and implementation of the solution found in next cycle of research4. Summary of work: Five modules of pre-clinical phase (Spiral-1) of AJKMC Curriculum have been delivered to the class of 2016 till Aug. 2012. The first spiral of undergraduate medical curriculum deals with foundation of medicine (normal structure & function) along with introduction to professionalism, contemporary biomedical ethics, history of medicine, culture, diversity, sanctity of human body/tissue, information & communication technology, learning styles & approaches, small group dynamics, theories of learning, assessments, reflective writing, and legacy of Muslim physicians. The learning outcomes, curriculum map, teaching and learning strategies and blue print for assessment was provided to faculty and students in Study Guide of each module. All the modules delivered so far were evaluated for their effectiveness and impact on students learning at the end of each module by a semi-structured questionnaire on a 5-point likert scale, having open ended comments column also5. Summary of results: Triangulation of quantitative & qualitative data revealed positive perceptions of students about integrated curriculum. The response rate of voluntary participants was 92%. Ninety six % participants graded all sessions as good/excellent/outstanding; whereas 1% poor and 3% fair. Content analysis of qualitative data revealed following recurrent themes: Need for integration highly valued; Delivery of curriculum can be
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further improved; Faculty is supportive; Small group sessions encourage individual participation and active learning; Self directed learning prepares for life-long learning; Logistics should be improved. Conclusions: Integrated curriculum provided holistic approach to human problems and enhanced students’ motivation for patient centered and self-directed life-long learning6. Take-home messages: Integrated curriculum can be implemented in a resource constrained environment; however, it demands a strong institutional leadership role.
A-291Folk medicine in Turkey
K. Hüsnü Can BASER1, Ekrem SEZIK2
1 Formerly Anadolu University, Faculty of Pharmacy, Department of Pharmacognosy, 26470 Eskisehir, Turkey; 2 Gazi
University, Faculty of Pharmacy, Department of Pharmacognosy, Ankara, Turkey
Turkey is a country having land in two continents: Asia and Europe, rich in botanical and cultural diversity. Flora of Turkey is rich and diverse with over 12.000 flowering plant taxa recorded enjoying an endemism record of over 30 per cent. It is a land which hosted numerous civilizations and has witnessed various successes in medicine, agriculture, history, etc. Cuneiform tablets dating back to Assyrians, Sumerians and Hittites are witness to the use of many medicinal plants which are still used in the same way. Hippocrates, Galenus, Theophrastus and Dioscorides contributed to the creation of a traditional form of medicine in Anatolia 2000 years ago. Their followers like Avicenna, Rhazes, Biruni, Ibn Baitar, etc. perfected medical and medicinal practices before the rise of the Renaissance. Their teachings influenced medical practices in Turkey through the Seljukian and Ottoman era. Apart from the discovery of medicinal plants of Anatolia by the natives, many nations settling in Anatolia brought along their culture and practices as well as the knowledge of medicinal plants to enrich the already wealthy culture of the land. The paper intends to to shed light to folk medicinal practices at work in today’s Turkey with examples such as the Ocak (read Odjäck) system which is thought to be evolved as a result of islamic influences on Central Asian shamanic rituals.
A-292Influence of motor activity on health condition of students in Avicenna Tajik State Medical University
Sh.Sagdieva 1
1Avicenna Tajik State Medical University, Rudakiave, 139, Dushanbe, Tajikistan
Objective: To study the influence of impellent sport activities on students’ health condition. Methods: The health level estimation of students has been studied. The health level estimation was studied using Rufye index in 2 groups of students: 1) students visiting sport sections and living active physical life. 2) students with no active physical activity. Results: The comparative analysis of the results has shown that none of the groups had achieved excellent results. The good result in group of the boy students visiting sports sections was 9.1% and in control group 0%. The satisfactory result in investigated group was 63.6% against 72.2% in control. The unsatisfactory result in group of sportsmen was 27.3% comparing to 72.7% in group of the students ignoring physical activities. Girls groups couldn’t achieve
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perfect and good results at all. Satisfactory results had been registered in 36.4%, and the unsatisfactory result were 63.6% in group of girls without regular physical activities. The group of the city girls who have regularly physical activities, included only 4 persons aged 18–20 years, and among them there were no unsatisfactory results. Conclusion: The preliminary results of our research confirm scientists opinion on a tendency to deterioration of students’ health condition. The majority of students, who ignore physical activities, have got proved unsatisfactory health condition.
A-293Innovative Strategies in Medical Education and Challenges faced in Establishing Gulf Medical University in the Middle East – A Case Study
Vinod Abraham1 1Director - External and International Affairs, Gulf Medical University, Ajman, U.A.E.
The Gulf Medical University (Formerly Gulf Medical College) and G.M.C. Hospital & Research Centre, Ajman, established in 1998 has been involved in undergraduate education in Medicine, Physiotherapy, Dentistry, Pharmacy and Premium Healthcare delivery since the last decade. The Gulf Medical University is in its 14th year of providing quality Medical Education and is recognized as an institution of higher education by the Ministry of Higher Education & Scientific Research, U.A.E. The University receives students from 50 countries around the world. The GMU also is proud of its Innovative Strategies in Medical Education; Organ System Based Curriculum developed and implemented by GMU in U.A.E.. Curriculum to feature simulation based clinical training. Unique Summer Training Programs and the worldwide network of institutions to which the students can do their Summer Training and Internship. The University is also a trendsetter in hosting the Regions first Dedicated Health Communications Divisions publishing a Health Magazine in English & Arabic and the Regions First Conference Secretariat which has done over 400 Major Conferences, Seminars, Workshops and other CME events. The Gulf Medical University has innovated an ambitious plan of introducing Short Term Courses and Certification programs targeted at the UAE’s working medical and allied health professionals. This presentation aims to highlight a case study on the Innovative Strategies in Medical Education and Challenges faced in Establishing Gulf Medical University in the Middle East.
A-294Knowledge, Attitude and practice of medical ethics of faculty of a medical university, in Karachi, Pakistan
Zulfiqar Ali Shaikh1, Masood Hameed Khan2, Dur Muhammad Shaikh3, Fahmeeda Khatoon4, Baqar Ali5, Umer Bashir6, Hassan Shabir7 1MBBS, MSPH, MBE; Associate Professor, Community Medicine, 2FCPS, FRCP, PhD; Professor, Medicine, 3MBBS, MPhil;
Assistant Professor, Pathology, 4MBBS, MPhil; Lecturer, Bio-chemistry, 5-7Medical Students, Final Year, Institute: Dow
University of Health Sciences, Karachi, Pakistan. Correspondence: Dr Zulfiqar Ali Shaikh, Community Medicine Department,
Dow International Medical College / Dow University of Health Sciences, At Ojha Campus, SUPARCO Road, Karachi, Pakistan.
Email: [email protected], Contact # +92-333-2356234
Background: The ethics in medical practice is as old as the profession itself. In many countries
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bioethics is part of medical curriculum. Due to increased public health awareness, complaints against unethical behavior of medical students and professionals are proliferating. Though bioethics has gained priority in formal medical education, but is not much recognized everywhere. To design an ethical curriculum, the first step is to assess the basic knowledge and attitude of medical teachers regarding bioethics, and second to well equip them to impart same skills to the students to workout ethical dilemmas. This study attempted to explore the knowledge about medical ethics in a Medical University of Karachi, Pakistan. Objectives: To assess knowledge, attitude and practice of medical teachers regarding medical ethics, and to sensitize the academicians for training of the teachers and making bioethics an integral part of the curricula. Results: All of the study participants conducted research in their field of specialty; to get promotion (71%), get funding (9%), discover new ideas/theories (4%), for personal interest (5%), and multiple reasons (11%). Among them, 16% had received training of research / bioethics, and only 2% got refresher training. Eighty two percent knew the existence of ethical review committee in their institution; 55% got prior approval from ERC, 32% declared conflict of interest, 17% taught/discussed medical ethics with students; 21% took informed consent, 35% had idea of vulnerable population, 43% did not want to give full information to the study participants, and 70% recognized difference between medical research and care. According to 77%, the authors’ list included only those who had contributed, but 17% also included supervisors/higher seniors and 6% colleagues. Conclusion: Health care providers frequently encounter ethical issues. The medical teachers were not fully equipped with appropriate knowledge of bioethics. It should be included in curriculum and teachers be trained for the same.
A-295QOL assessment and proposed scale for evaluation of traditional medicines
Nobuo Yamaguchi1,3 , N.Amat 2, Y.Delitiat 2 1Medican Research Institute of Xinjiang Medical University (830011); 2Uighur Medicine Department of Medical University Road
No. 393, Urumqi City , Xinjiang Uighur Autonomous Region , China. Visiting Professor, Xinjiang Medical University, Urumqi,
China; 3Ishikawa Natural Medicinal Products Research Center, Kanazawa, Ishikawa, 920-0167, Japan
According to the reports from Ministry of Health in each country, the average life spans were expanded over the past century. This trend is expected to be extended further in the future and at the same time continuous efforts should be made. That indicates that the quantitative objective, as one of the objectives people have aimed at since ancient times to attain the perpetual youth & longevity, are almost achieved in this way. However, as the next objective, it is necessary for us to be concerned about improving the quality of life.
A-296Search for bioactive compounds to inhibit plant pathogenic organisms
Narong Singbuaraudom1
1Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
Objective: One hundred Thai medicinal plants were evaluated for their in vitro anti-plant pathogenic activity. Methods: Ethanol crude extracts were obtained and tested for anti mycelia growth by dilution
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methods. The crude extracts that exhibited effective concentration lower than 10,000 ppm were selected for isolation of the active compounds by column chromatography. Active fractions were screened by diffusion method. The structures of the isolated compounds were characterized by NMR techniques. Results: The results of this investigation are as follows; (1) the crude extract from Alium sativum was highly effective in inhibiting plant pathogens, presumably due to allicin. Since allicin is unstable it represents a low potential for development as commercialized product (2) berberine, the alkaloid isolated from Coscinium fenestratum (Gaertre) Coleb exhibited specific ability to inhibit the grape scab fungus, Sphaceloma ampelinum, and banana wilt, Fusarium oxysporum ssp. cubense. This extract is now in the process of developed for commercialization in the name Natural-A .(3) chavicol, isolated from Piper betle L., was found to exhibit a broad spectrum to inhibit plant pathogenic organisms. It is one of the commercialized products which was name Natural-B. The other members of Piperaceae, namely Piper niqrum and P.chaba, also contained bioactive compounds. Conclusion: The result of fractionation revealed that these active compounds are different from those found in other members of this family
A-297Secondary metabolites from a Türkish marine sponges
B.Konuklugil, 1 P.Proksch, 2 B.Gözcelioğlu 3
1University of Ankara, Faculty of Pharmacy,06100- Tandoğan, Ankara, Turkey; 2Heinrich-Heine-Universität, Institute of
Pharmaceutical Biology, Universitätsstr. 1, 40225 Düsseldorf, Germany; 3Tübitak, Ankara, Turkey
The sponges represent rich source of secondary metabolites having a great diversity of structures and bioactivities. They are even considered to be more prolific producers of new marine natural products. Until now, more than 5000 different compounds have been isolated from about 500 species of sponges and every year hundreds of new compounds are being discovered. Most bioactive compounds from sponges can be classified as anti-inflammatory,anti-tumour, immuno- or neuro-suppressive, antiviral, antimalarial, antibiotic and antifouling (1-4). Turkish coatline totals almost 8400km. The sponges found in Türkish water, have not been intensively studied yet. Chemical investigation of methanolic extract of the sponges led to isolation of furanosesterterpene, named fasciculatin, heptaprenylhyroguinone, avarol, avarone, oroidin, 4,5-dibromopyrrol-2-carboxylic acid, and 25-hydroxy-24-methylcholesterol, agelasine C, agelasine D and cylindradines A. The compounds were purified by “coloumn “ chromatograpy and their structures was characterized using NMR spectroscopy. Some of these compounds were tested for antioxidant, antimicrobial; anticholinesterase; and anti-trypanosomal activities. Futher studies are in progress.
A-298Study of Prevalence of Asthma in male workers of Leather Tanneries in Lahore, Pakistan
Ali, Sajid, Shehzad
Objective: This study aimed to estimate the prevalence and to identify some risk factors of adult asthma in male workers in Lahore, Pakistan. Methods: A cross sectional study was conducted from August 2010 to March 2011 on leather tannery workers of Lahore, Pakistan. Data were collected from
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641 workers engaged in 95 different tanneries in industrial area selected as sample of convenience. Face to face interviews were performed using a structured pre-tested questionnaire by trained data collectors. Results: Prevalence of adult asthma was 10.8% (69/641) in this study population. The prevalence of perceived work-related asthma was 5.3% (34/641). Multivariable logistic regression model showed that after taking into account the age effect, the leather tannery worker were more likely to be asthmatic, if they were illiterate (adjusted OR = 2.13, 95% CI: 1.17–3.88), of Pathan ethnicity (adjusted OR = 2.69; 95% CI: 1.35–5.36), ever-smoked (adjusted OR = 2.22, 95% CI: 1.16–4.26), reportedly never used gloves during different tanning tasks (OR = 3.28; 95% CI : 1.72–6.26). Also, the final model showed a significant interaction between perceived allergy and duration of work. Those who perceived to have allergy were more likely to have asthma if their duration of work was 8 years (adjusted OR = 2.26; 95% CI: 1.19 – 4.29) and this relationship was even stronger if duration was 13 years (adjusted OR = 3.67; 95% CI: 1.98–6.79). Conclusion: Prevalence of asthma in leather tannery workers appears to be high and is associated with educational status, ethnicity, smoking, glove use, perceived to have allergy and duration of work.
A-299Some questions of the development of Uzbek public medicine and practical aspects of it on the public health of the population
M.Khamraev The academy of national medicine of Uzbekistan
The modern medicine has huge luggage of worked out procedures, doctrines, technologies. But the advertised medicines can not cure us from all deceases at once. In this connection necessity for formation the concept of maintenance of health has matured, in which both reforms of measures on prophylaxis and avoidance of diseases, and management on incorporation in life of experience of the Uzbek people would be reflected. The important moment in work should be a complex of improving measures, which also includes daily work on informing the population about the able-bodied mode of life, about methods of right self treatment, about the economic significance of health.We will discuss the the purposes, problems and aspects of the National Medicine Program of Uzbikistan.
A-300YNSA as a new acupuncture treatment for pain killing
Hegyi Gabriella1
1 Head of CAM Department of PECS Medical University, Hungary, Director of Yamamoto Rehabilitation Institute, Budapest, HU
(affiliated to PECS Med. Uni.)
Introduction: Patients treated with YNSA often experience long-term relief from symptoms. Objective: To evaluate the effectiveness of YNSA for the relief of pain of the locomotor system. Design, Setting, and Patients: Prospective series of 2100 patients with severe movement-associated pain who were treated with a single application of YNSA via basic points and Y points selected according to the palpation results of a neck diagnosis. Depending on the duration of topometry, the needles were left in position for 3-9 minutes, or was used an unique permanent technique ( absorbeant monofilament with a
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special designed needle to inset it) An objective measurement was used to record patients' movements by an external computer-controlled ultrasonic emitter with an accuracy in the millimeter range. The participants described their subjective sensation of pain before and after treatment via a visual analog scale (VAS) for pain. All patients were interviewed a few weeks later to document the success of the treatment by independent MDs.Main Outcome Measures: The speed of YNSA efficacy and its enduring effects, the complaints for which it is particularly effective, and whether YNSA is suitable for emergency application. Results: All together, 91,2% of the patients regarded a single, brief application of YNSA as effective. The average VAS score before YNSA was 63/100; afterward the average was 19/100. A total of 51% of the patients stated that they experienced complete relief of symptoms subjectively and by using the VAS. A total of 43.3% of the patients (n=45) experienced some relief after YNSA. Seven patients did not subjectively experience any effect. A total of 52.5% of the patients experienced both objectively measurable (topometry) and subjectively stated relief or complete elimination of symptoms (on VAS), lasting from 1 hour to 382 days. Overall movement after YNSA remained constant in topometric measurements for 35.1%. Overall movement for 6,6% measured topometrically was worse after YNSA than before. Conclusions: YNSA can be an effective treatment especially for pain of the locomotor system originated from different reasons and can provide subjective improvement for most patients. Due to its ease of application and its reliability, YNSA is particularly well suited for use in emergency treatment since a good long-term effect can be achieved by leaving the needles in position for just 3-9 minutes or more time in form of a permanent application for patients.
A-301Prevalence of gout in Pakistan
Sajid
This study was conducted to assess the prevalence of gouty arthritis in 100 patients (both male and female) between the ages of 35 and 70 years. The patients that were randomly enrolled in this study The mean age of the 66 male patients was 53.59 with a standard deviation of 11.34 and the mean age of the 34 female patients was 58.59 with a standard deviation of 8.19. Consequently, this indicated high prevalence of gout in male than in female. It can be seen that between 35 and 40 years of age, the total number of patients was 11; between 40 and 45, it was 12; between 45 and 50, it was 13; between 50 and 55, it was 13; between 55 and 60, it was 15; between 60 and 65, it was 19; and between 65 and 70 years of age, it was 17. However, this clearly indicated that prevalence of gout was high in patients that are above 50 years of age.
A-302Traditional Uighur Medicine theory of "KuWat" (energy) classification and its function
T.Tursun 1，R.Kawuz 1
1Department of Uyghur Medicine, Xinjiang Medical University, Urumqi, 830011, China; *Correspondence should be addressed
Theory of "Kuwat",is one of the basic theory of Uighur Medicine, Is a description of human power
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definition, types and functions. Dimensional medicine in force, refers to maintain and advance the human mental and physical Major Factor. According to the " base " of the emergence and existence of position and action,its divided into two energetically，The former is ( Kuwat ) and the latter ( Kuwat ). KuWait ( former energy )，The former is ( Kuwait ) the creation and existence of the position and role, divided into vitality, spiritual force and natural force three categories. First, Kuwait is energy of life, vitality, refers to promote heart beating force. It is produced in the heart, derived from the heart, for the normal function of the heart service, and through the heart of the activities and complete, small blood circulation, for the maintenance of normal human survival play an important role in. Although people's joy, anger, grief, joy and emotion control ability is the effort to complete, but these mental phenomena in actual performance in the heart. Second，Kuwait is energy of spirit，mental stress, refers to the generating and directing all intelligence and force. It is produced in the brain, is impels all spiritual center.
A-303Experience of «RAWAN» application at sick children with bronchial asthma in Turkmenistan
Dmytriy Sebrebryakov1
1Turkministan State Medical University, Ashkabad, Turkministan
Bronchial asthma (BA) belongs to number of widespread allergic diseases at child age. During the last years all over the word. including Turkmenistan, there is marked a tendency to increase in prevalence of bronchial asthma at children and to its more serious course. Bronchial asthma is a disease which having begun at children, often proceeds at mature age, becoming a cause of physical inability. In this connection, the problem of prevention, diagnostics and treatment of this disease acquires a great value. The purpose of this research was application of «RAWAN», made in Peoples Republic of China by pharmaceutical firm "Ha-Bo", Urumchi SUAR, possessing property to strengthen «Wei-ci», inmmune system, to eliminate weakness, constant hyperhidrosis, propensity to catarrhal diseases ,chill, tickling and discomfort in a throat. There were surveyed 45 children at the age from 5 to 14 years. At 40％of patients there diagnosed BA of moderate severity, at 56％-light course. At the majoriy of children with BA it was combined with food medicinal allergy, pollinosis, atopic dermatitis, allergic rhintis. Alongside with a basic treatment, there was appointed «RAWAN», three times a day of 3-5gr.dissolved in warm boiled water. Course consisted of 25 days of treatment. In the research there were incluled sickly and long ailing children with BA at which it was marked more than 4-6 episodes of ARVI in a year, accompanied by BA aggravation. There was carried out monitoring of following indicators: serum interferon and α-interferon, and also clinical dispalys of the disease. At the picture there is presented a level of interferon at children receiving «RAWAN» there is noted decrease of serum interferon, initially raised, upon termination of therapy(p<0.01) and increase of production of α-interferon(p<0.001) to the termination of a complete of therapy. Clinical signs and sysptoms at children with bronchial asthma, receving«RAWAN». After the termination of therapy with inclusion of «RAWAN». frequency of revealing of the specified symptoms authentically decreased(p<0.01-0.001) in comparison with the revealed symptoms of initiation of treatment, It is necessary to notice that at application of «RAWAN». At one of children receiving a medicine, it is noted negative collateral and allergic displays. Thus, inclusion in complex therapy of patients with BA of medicine«RAWAN»,has allowed to gain convincing clinical
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effect, The received results on medicine application in treatment of patients with BA, are represented rather perspective. Medicine dose not cause collateral and allergic reactions, changes in gastroenteric tract of observable children at medicine application. Against medicine application production increase of production of α-interferon initially lowered (p<0.001),and decreased in the raised level of serum interferon.
A-304Clinical effecacy observation Uighur Medicine Preparetion Denar on the patients with viral hepatitis with Cholestatic syndrome
Dmytriy Sebrebryakov1
1Turkministan State Medical University, Ashkabad, Turkministan
The treatment of cholestatic forms of viral hepatitis is the one of the complicated question in hepatology, the purpose of this reasearch is application of the Uighur Medicine Preparetion Denar decoction treatment the hepatitis with Cholestatic syndrome, Denar decoction can reliefe the fever of the liver, remove toxin, remove sputum, functionging as a choleretic , laxative and uriginative agent. There was 39 patients with chronic viral hapatitis with cholestatic sysndrome was under observation. The 15 of them were with chronic viral hapatitis HBV+HDV, 18 with the chronic viral hapatitis HBV+HCV, and 6 with the chronic viral hapatitis HBV.The diagnosis was verified by determination of infection marker of viral hepatitis.Along with the basis treatment the “Denar ” was prescribed three times a day by 3-5g deluated with warm water. The course of therapy consisted of 21 days. The control group conisited of 20 patients with the cholestatic form of chronic viral hepatitis during the basic therapy. The effeciency of therary was evaluated by laboratory data ,there was noted amelioration during the usage of “Denar”. Many of the patients faster then in control. Lowering of the skin itch intensity, normalize sleeping. During the treatment, Ictertitiousness of the coverlet sypntom of 30 of patients had decreased or then disappeared, and fast reverse dynamics of biochemical tests, for the 28 patients the biochemical markers was improved, also correlated with immune parameter wa normalized. 9 patients at the end of treatment had the marker of the viral hepatitis decreased or show negative (superficial antigin of viral hepaptitis HBV, antibodies IgM to the heart shaped antigen of viral hepaptitis HBV in the immune-enzyme analysis) and there was no datected nucleic acids of viruses in polymerase chain reaction. We also found that can not find any side effection and allergic affection. Thus, the inclusion to the complex therapy preparation “Denar” allowed to get convincing clinical effect. The obtained results in application of this medicine in treatment of viral hepaptitis with cholestatic syndrome syndrome present itself very prespective.
A305Epidemiological analysis of vitiligo according to traditional Uyghur medical theory and western medicine
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K.Tuohuti 1, R.Shawuer 1*1Hotan prefecture traditional Uyghur medical hospital, Xinjiang autonomous region PRC，hetian City，848000, correspondent to [email protected]
Objective：To analyze Epidemiology of vitiligo according to traditional Uighur medical theory (one of the four big traditional medical systems next to TCM, Tibet and Mongol in china. an organic combination of traditional medical system in Islamic east ,china with Far East) and western medicine. Method: with 80 clinical cases we used Uyghur medical theory about albino pathology and epidemiological parameter (age, sex, family history, work place- Job and regional difference ) of western medicine. Result: 1. according to pathology analysis albino with help of humour theory of traditional Uighur medical system it seems people with phlegmatic structure especially with tasteless phlegm (temsiz belgam) and salty phlegm (tuzluq belgam). 2. People with eat habit cold nature foods example (yoghurt) has more clinical cases 3. During the school age girls have high risk. Vitiligo cases seem more frequent in girls than boys. 4. Relationship with job. Intellectuals have more cases than works and farmers. Teachers and pupils are high risk groups.4: regional difference: following is regional frequency comparison among four places. Hotan < Aksu < kashgar < ili. Conclusion: systematic epidemiological finding is important to prevent and cure vitiligo. Our research would be some contribution to cure vitiligo in parts of undeveloped regions in china and other world with herbal medicine.
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