p159. nell-1 enhances bone formation in rat and sheep spinal fusion models
TRANSCRIPT
156S Proceedings of the NASS 22nd Annual Meeting / The Spine Journal 7 (2007) 1S–163S
CONCLUSIONS: While the rate of device-related complications for both
groups of anterior cervical patients was low, ACDF had more second
surgical procedures requiring supplemental fixation than the BRYAN Cer-
vical Disc, which by its design could not have a non-union or pending
non-union. In regard to all other complications, the two types of anterior
cervical surgery devices used in this study were found comparable and
not statistically different.
FDA DEVICE/DRUG STATUS: Bryan cervical disc: Investigational/ Not
approved; Cervical plate: Approved for this indication.
doi: 10.1016/j.spinee.2007.07.368
P159. Nell-1 Enhances Bone Formation in Rat and Sheep Spinal
Fusion Models
Steven Lu, MD1, Julie Whang, DDS1, Chia Soo, MD1, Xinli Zhang,
MD,PhD1, Tara Aghaloo1, Ahmet Alanay, MD2, Antonia Napoli2,
Benjamin Wu3, Shelby Hiles, BS1, Simon Turner, BVSC4, Kang Ting, DMD,
DMSC1, Jeffrey Wang, MD5; 1UCLA Dept of Craniofacial Research, Los
Angeles, CA, USA; 2UCLA Dept of Orthopedics, Los Angeles, CA, USA;3UCLA Dept of Bioengineering, Los Angeles, CA, USA; 4Colorado State
University - Dept of Veterinary Sciences, CO, USA; 5UCLA Dept of
Orthopedics, Santa Monica, CA, USA
BACKGROUND CONTEXT: Osteogenic growth factors such as bone
morphogenic proteins (BMPs) are increasingly being used as bone graft ex-
tenders to enhance the success rate of spinal fusion. However, many of them
have extended roles in cell line growth outside of bone formation. BMP2 and
BMP7, approved for use in humans, have been associated with adverse reac-
tions such as local inflammation, ectopic bone formation, and nerve compres-
sion. Researchers have been searching for alternative growth factors which
act specifically in the pathway of bone formation. Nell-1 [Nel-like mole-
cule-1; Nel (a protein strongly expressed in neural tissue encoding epidermal
growth factor like domain)] is a novel secretory molecule originally discov-
ered in the prematureyl fused sutures of patient with craniosynostosis. Nell-1
is directly regulated by Runx2, the master regulatory gene controlling bone
formation, and targets those cells committed to an osteochondrocytic lineage.
PURPOSE: The purpose of this experiment was to study the ability of
Nell-1 protein to successfully promote spinal fusion in a rat and sheep
model.
STUDY DESIGN/SETTING: In vivo study of Nell-1 protein in rats and
sheep.
METHODS: Two well understood spinal fusion models were used to
study the osteogenic properties of Nell-1. First, twelve athymic rats were
divided into three groups and implanted with sheep DBM alone, sheep
DBM with 5ug Nell-1 and sheep DBM with 10ug Nell-1. These rats were
sacrificed at 4 weeks. Manual palpation was used to determine spinal fu-
sion. Adjunctive radiographic and histological evidence was obtained to
study detailed bone formation. Secondly, due to the biomechanical similar-
ities of sheep and human spines, a pilot study was conducted consisting of
eight sheep. They were divided into four groups: sheep DBX alone, 0.3
mg/ml of Nell-1, 0.6 mg/ml of Nell-1, and 1.5 mg/ml of Nell-1. The ani-
mals were sacrificed at 3 months and computerized tomography in addition
to plain radiography was obtained to assess for bone formation.
RESULTS: Compared to rat spines containing sheep DBM alone which
had no fusion at 4 weeks, rats implanted with 5ug and 10ug of Nell-1 dis-
played fusion in 50% and 75% of the spines, respectively, as determined by
manual palpation. This difference was evident on radiographic studies by
microCT and Faxitron as well as histologically. MicroCT images verified
enhanced compact bone formation especially at sites adjacent to the trans-
verse processes. Stained histological sections demonstrated early integra-
tion of sheep DBM into bone remodeling and active bridging between
bone fragments. Our pilot sheep studies also confirmed accelerated bone
formation within sheep spines implanted with sheep DBM and Nell-1.
Two month CT images showed callus formation and intervertebral spacer
filling over control with sheep DBM alone, particularly at the dosage of
0.6mg/ml Nell-1.
CONCLUSIONS: Our experiments in rat and sheep spinal fusion demon-
strate enhancement of bone formation when Nell-1 protein is incorporated
into sheep DBM. Most importantly, Nell-1 acted specifically at the site of
implantation and did not exhibit ectopic bone production or swelling. This
is likely due to the inherent nature of Nell-1 as a downstream growth factor
of those cells already committed toward the osteochondrocytic pathway.
Therefore, Nell-1 may serve as an effective growth factor in spinal fusion
with less potential less adverse effects.
FDA DEVICE/DRUG STATUS: This abstract does not discuss or include
any applicable devices or drugs.
doi: 10.1016/j.spinee.2007.07.369
P160. An Investigational Study of Nucleus Pulposus Replacement
Using an In-situ Curable Polyurethane: An In Vivo Non-human
Primate Model
Bryan W. Cunningham, MSc1, Nianbin Hu, MD1, Helen J. Beatson, BSc2,
Paul C. McAfee, MD3, Hansen Yuan, MD4; 1St. Joseph Medical Center,
Towson, MD, USA; 2Baltimore, MD, USA; 3St. Joseph Hospital, Towson,
MD, USA; 4SUNY - Upstate Medical University, Syracuse, NY, USA
BACKGROUND CONTEXT: N/A.
PURPOSE: The current study was designed to evaluate the biodurability
and elicited histopathological response of an in situ curable polyurethane
nucleus pulposus replacement following long-term implantation in a pri-
mate model.
STUDY DESIGN/SETTING: N/A.
PATIENT SAMPLE: N/A.
OUTCOME MEASURES: MRI, Biomechanics and Histology.
METHODS: A total of 14 mature male baboons were randomized into
a six-month (n57) and twelve-month (n57) postoperative time periods.
Each animal underwent a lateral transperitoneal surgical approach fol-
lowed by nucleotomy at the L3/4 and L5/6 levels. The L5/6 level was im-
planted with the DASCOR� device having an elastic modulus similar to
that of the natural disc. The L3/4 served as a surgical (nucleotomy) control
in each case. Post-mortem analysis included MRI assessment of implant
position and Modic Type endplate changes (0–III), multi-directional flex-
ibility testing, systemic histopathology and undecalcified histology.
RESULTS: All animals survived the procedure and postoperative interval
without significant peri-operative complication. Post-mortem MRI analysis
revealed Modic Type I changes in 5/7 nucleus replacement levels versus 0/7
in control levels, which were identified as Modic Type 0. For the twelve-
month group, Modic Type I changes were identified in 3/7 nucleus replace-
ment levels versus 0/7 in the respective control levels. Intervertebral hydra-
tion signals at the operative and control levels were markedly reduced at both
time intervals. Multidirectional flexibility testing indicated no difference in
axial rotation (pO0.05) for the six and twelve-month groups, when compar-
ing the two surgical motion segment groups and intact spine. Flexion-exten-
sion and lateral bending exhibited reduced segmental motion for the nucleus
replacement and operative control levels at six-months versus the non-oper-
ative intact spine (p!0.05). By twelve months, flexion-extension motion at
the nucleus replacement level was restored to intact condition (pO0.05).
Histopathologic analysis of the nucleus replacement treatments exhibited
increased densification of trabeculae along the endplate periphery, corrobo-
rating the Modic Type I changes observed radiographically. No change in
densification or endplate sclerosis was observed at the L3-L4 surgical control
levels. Histopathology of all local and systemic tissues demonstrated no
evidence of significant pathological changes.
CONCLUSIONS: Using a non-human primate model, the current study
investigated the in-vivo response to nucleus pulposus replacement follow-
ing nucleotomy. Modic Type I changes were observed to a greater extent at
the operative versus control levels, and these changes appeared to reduce
from the six-month to twelve-month intervals. The Modic changes
observed at the implanted sites are considered normal and have been docu-
mented in a number of in vivo non-human primate studies. Multidirec-
tional flexibility testing indicated restoration of segmental motion for the