pacbio - galaxy · unmethylated (bottom) adenosine. (b) typical smrt sequencing fluorescence traces...
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PacBio
2003
Levene et al. 2003
Korlach et al. 2008
Eid et al. 2009
Flushberg et al. 2010
Stats
‣ Mean RL ~ 3,000 bp ‣ 95th percentile ~ 8,000 bp ‣ Max RL ~15,000 bp ‣ 30,000 - 50,000 reads ‣ 100 - 150 Mb/ SMRT cell ‣ Runtime 90 min
Types of runs
‣ CLR: Continuous Long Reads ‣ CCS: Circular Consensus Sequencing
Error profile and GC bias
Carneiro et al. 2012
Carneiro et al. 2012
Loading regimes
‣ Diffusion loading ‣ Magnetic bead loading
Modification detection
Flusberg et al. 2010
Error correction
Koren et al. 2012
Error correction
Koren et al. 2012
Closing gaps
Chaisson et al. 2015
Closing gaps
Chaisson et al. 2015
Transcript sequencing
Tilgner et al. 2014