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mage Analysis Algorithms

Brightfeldositive Pixelount

Calculates the contribution of each stain atevery pixel location in the image.

olorDeconvolution

Separates the image into di erent layers,corresponding to your stains.

ytoplasmictaining

Measures intensity and provides the percentageof cells containing stain.

MembraneAnalysis

Detects membrane staining for individual cellsand identi es the intensity and completeness of

the staining.

uclearAnalysis Detects nuclear staining, and intensity.

MicrovesselAnalysis

Detects and "uanti es microvessels stained#ith endothelial mar$ers.

%are &ventDetection

'he algorithm uses color segmentation andmorphological image processing methods to

detect and count ob(ects of interest.

luorescetyto)!uclear+

Designed to measure both cytoplasmic andnuclear intensity on a cell)by)cell basis.

b(ectolocali-ation+

Capable of measuring ob(ect properties determining colocali-ed ob(ects.

Microvessel *+ Designed to count vessels measure vesselproperties.

Areauanti cation

/uanti es multiple 0orescent dyes on an areabasis.

Contact In ormation

The Director o the Stem Cell Pathology Unit: Alexander !i$itin, MD, PhD

rofessor of Pathology

athology: Andre# Miller, D1M, Dipl. AC1PAssistant Professordm234cornell.edu

resa Southard, D1M, Dipl. AC1P, PhDAssistant Clinical Professorls564cornell.edu

lide Digitization:Chris Pelletier, %esearch Support Specialistlp764cornell.edu

or 8eneral /uestions and Additional 9nformation:ebsite: stemcell.cornell.eduor$ State Stem Cell Science?!>S'&M@*or more information visit: Stemcell.ny.gov

The Stem Cell Pathology Unit

cm tall

23. cm #ide

Animal Phenoty ing

;e o er comprehensive animal phenotyping #itha focus on the assessment of stem cell niches. StemCell Pathology is a rapidly emerging discipline thatprovides critical lin$s bet#een animal modeling,intravital, and histopathological analysis. ;einvestigate the conse"uences of geneticmanipulation in stem cells and determine the roles of stem cells in regenerative medicine and in thepathogenesis of various diseases, such as cancer.

ur goal is to foster interdisciplinary collaborationto facilitate the advancement of stem cell research. 'he Cornell =niversity Stem Cell Pathology =nitprovides comprehensive pathology services andplanning advice for all pro(ects, #ith an expert focuson stem cell related pro(ects.

Pro!ect Planningur pathologists o er advice on the selection of the

most appropriate animal strains and tissue xativessuitable for your particular studies. ;hen you are in theprocess of experimental design, #e can help you selector design the most appropriate genetically modi edanimals for your needs and o er our advice on the mostbene cial necropsy for comparative analysis.

Pathological Assessment 'he assessment of disease pathogenesis by trained

pathologists is critical for stem cell research. ;e o erthe expertise to help you identify anatomically locali-edstem cell niches during disease initiation andprogression. ur Stem Cell Pathology =nit combinescomprehensive animal phenotyping services #ith thespeciali-ed assessment of stem cell niches.

Interdisci linary Colla"oration*or more information, please visit our #ebsite at: *or more information, please visit our

#ebsite at:stemcell.cornell.edu

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1arious topics in regenerative medicine, such as then situ contributions of stem cells to normal andathologic regeneration, or organismal reactions to theransplantation of various bioengineered constructs,e"uire the support of dedicated pathologists. urathology Services can give you accuratenterpretations of novel ndings and help #ith thealidation of model systems.

Pathology Ser#icesCornell*aculty

Murine necropsy tissueollection ?no histopath@

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Gright) eldScanScope

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