[paraj_2014] reverse micellar extraction of fungal glucoamylase produced in

7/26/2019 [Paraj_2014] Reverse Micellar Extraction of Fungal Glucoamylase Produced In

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December 2014Vol. 24No. 12

J. Microbiol. Biotechnol. (2014),24(12), 16901698http://dx.doi.org/10.4014/jmb.1407.07016 ResearchArticle jmbReview

Reverse Micellar Extraction of Fungal Glucoamylase Produced inSolid-State Fermentation Culture

, , -, , & , -,

, , - ,

Introduction

D

, 70%

35.

, ,

, ,

,

13, 19, 35.

(E)

, -

-- 12. E

, ,

-, ,

19, 23,

32. H,

, ,

, H

,

, , ,

5.

, , ,

. H,

3, ,

(GA) E.

I ,

(48112 D)

,

,

* +-

+-

- .

-, -

- , , (). ,

, /

.

(.., ,

-2--- () )

.

2.75, 100 , 200 . ,

(/)

2 3, . , 60%

, 140%

3.0-.

, - , , , ,


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1691 Paraj et al.

J. Mic robiol. Biotechn ol.

13.

, H,

23. ,

6, 8. ,

. I ,

33.

,

23. -

(., , , -

), E

. H,

24, 26, 27.

A ,

25

33% ,

25.

4, 25.

C GA

HLC 22,

HLC 29, HLC 28,

HLC 37, 10,

- 31, 34,

- 35,

36. H, ,

, ,

.

- , E. H,

,

. F ,

.

(CCD)

. F,

.

, ,

.

Materials and Methods

G

CCG 33991 (C C G,

) - -

(ICAN, I). 50%

(4 )

- ,

40 35C. A,

40C

5%, 4C.

(DFB).

A DFB

50% DFB.

100 ,

120 100 .

( , N. 2)

( 684 10 ).

.

, AO, -

( 115.9, 150, 200, 250, 284.1 M AO

). F ,

NC ( 15.9,

50, 100, 150, 184.1 M NC ) H

HC (1N) NOH (1N).

A, 4 3

20 . I

5 1,580 . ,

,

.

ME GA F. 1.

G

.

(0.1 ) (1.0 )

1.0 (1% , H 4.54.6) 60C

15 17. 15

(-E, I).

O (G)

1.0 17.

I

M 14. 40C

H 3 (0.006 /) . O


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Reverse Micellar Extraction of Fungal Glucoamylase 1692


(P) 1.0

/ .

18. A 0.015 / -

0.1 M H 5.0.

25-

. A- (1.0 )

0.1 , ,

20 40C. , 0.5

3,5-

10 . ,

1,580 10

530 . O

() 1.0 /

18.

L ,

21.

(G/) (/),

11.

CCD

, .

A (. 3, A I I., NC, A).

NC H , AO

, ME

1, 9.

1. F

, ;

, .

2. ()

. A - ,

,

() :

(1)

, 1.

B, B, B

- -

- , 7. B

, (0, 0, 0),

38.

A ,

.

A

,

(/) .

/ H,

+ + +=

Fig. 1. .

: G; : .

Table 1. ( ) ()

.

C

C () A ()

-1.682 15.9 115.9 2.16

-1.000 50 150 2.5

0.000 100 200 3.0

+1.000 150 250 3.5

1.682 184.1 284.1 3.84


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1693 Paraj et al.


AO NC . A ,

.

F ,

H, NC AO .P

. A ,

.

.

(., )

.

Results

G

- .

,

. G .

F. 2

200 A, 100 C,

H 3.0. F. 2A 2B 62%

H 2.5.

H, H 2.5 3.8

20%. F, C 80

,

60%

40% (F. 2A 2C). ,

A 120 280

, 210

(F. 2B 2C).

-

3. H

C (-

0.05) , A

( > 0.05).

A

,

.

( 1)

(% = 56.789 - 3.528+ 1.325 - 12.318 - 5.278 -

5.631 - 6.869

(2)

I - (

1)

(% = -226.018 + 0.352 + 0.927 + 140.208 - 0.002

- 0.002- 27.474

(3)

B - ,

Table 2. ()

,

,

.

.

C

(%)

GA (%)C

()

A

()

()

C

1 -1 -1 -1 52 71

2 -1 -1 1 28 80

3 -1 1 -1 56 76

4 -1 1 1 28 90

5 1 -1 -1 46 81

6 1 -1 1 20 84

7 1 1 -1 49 81

8 1 1 1 21 90

-

9 -1.682 0 0 50 24

10 1.682 0 0 38 91

11 0 -1.682 0 40 101

12 0 1.682 0 46 101

13 0 0 -1.682 58 102

14 0 0 1.682 21 105

C

15 0 0 0 53 108

16 0 0 0 55 110

17 0 0 0 58 114

18 0 0 0 58 113

19 0 0 0 57 116

20 0 0 0 55 104


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A . H 2.5,

H

2.5. ,

: 100 C,

200 A, H 2.75. A

51% .

I

(

/

)

E,

(., 100 C, 200 A, H 2.75).

F. 3. E

,

. /

0.5 3,

86% 130%

.

/ 0.5 3 (F. 3). I

0.5, 0.75, 1, 2, 3, 1.6, 1.8,

2.0, 2.3, 2.6, .

2.0

/ 2.0,

. F ,

/ 2.

Table 3.

.

D

F- -

C 1 170.0 170.0 19.7 0.001

A 1 24.0 24.0 2.8 0.126

1 2,072.2 2072.2 240.6 0.000

CC 1 367.6 367.6 42.7 0.000

CA 1 0 0 0 1.000

C 1 0.5 0.5 0.06 0.815

AA 1 420.8 420.8 48.9 0.000

A 1 4.5 4.5 0.5 0.486

1 635.6 635.6 73.8 0.000

9 3,464.8 384.9 44.7 0.000

() 3 2,266.2 755.4 87.7 0.000

() 3 1,193.7 397.9 46.2 0.000

(C ) 3 5.0 1.7 0.2 0.898

E 10 86.1 8.6

( ) 5 66.1 13.2 3.3 0.108

( ) 5 20 4

19 3,550.9

97.6%

A- 95.4%

Fig. 2.

. , , .

() . C A = 200 . ()

. A C = 100 . ()

. A C = 3. GA

10.9 G/ 2.72 /, .

A: A B (2-E) , GA:

G.


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1695 Paraj et al.


F. 4. I ,

.

100 C,

200 A, H 2.75.

.

107% 138% ( 30% )

1 4. H,

. C

, 3-

, 3-

.

I ,

. , (.,

)

. (100 C,

200 A, H 2.75, / 2), ,

F. 5.

-

130%. F,

60%

E. ,

,

(F. 5).

Fig. 3.

.

(GA) ( ) () 100 C, 200 A, 2.75. GA

11.6 G/ 2.97 /,

.

Fig. 4.

.

(GA) ( )

() 100 C, 200 A, 2.75, /=

2. GA 11.3 G/

2.70 /, .

Fig. 5.

.

(GA) ( ),

(), (), ( )

100 C, 200 A, 2.75, /= 2. GA

, , ,

12.9 G/, 120.88 /, 42.87 /, 2.21 /,

.


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Discussion

G,

, -

. C A H

.

I , H C

, ,

A .

H

1, 19. F,

, ,

5,

11.

. H,

, ,

H

A.

H 3.6, (48

112 D), H

A- . H,

.

19, 20.

I /

. ,

,

.

/

.

,

.

. ,

16,

9 13.514 D,

2. , (H 2.75),

. F,

50 D 4.0

15. , 60%

.

19, 30. A

, H

,

/

A.

I , -

E

. H, C A

2.75, 100, 200 , .

2

50% ,

140%

3.0-.

,

E.

Acknowledgments

I C

A &

I B G D

C E, I .

Equation Variables

B C

B L

B


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1697 Paraj et al.


References

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[paraj_2014] reverse micellar extraction of fungal glucoamylase produced in

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