parasitic nematode infective larva (l3) identification … poster.pdf · cooperia spp. (e.g. c....

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Head With sheath Without sheath Uneven tip Squared Chabertia ovina Oesophagostomum radiatum Bullet-shaped Haemonchus placei Rounded Bunostomum phlebotomum Nematodirus helvetianus X: ± 3.4 F: ± 20% F: 40 - 45% ± X: 5.4 ± X: 2.4 ± F: 50% ± Tail smooth F: 25% ± X: 5.1 ± 16 Intestinal cells Nematodirus battus X: 1.0 - 1.2 ± F: 0% Trichostrongylus axei X: 2.2 ± F: 10% ± Blunter tip Ostertagia ostertagi X: 2.0 ± F: 20% ± Cooperia spp. (e.g. C. pectinata) Finer tip X: 3.1 ± F: 20% ± Blunter tip Cooperia oncophora X: 8.3 ± F: 50% ± X: 5.2 ± F: 50% ± Tail notched/digitate 8 Intestinal cells Very long larva Finer tip Oesophagus rhabditiform Free-living nematode (see Fig.13) Oesophagus 40% of length Thin larva Strongyloides spp. ± 18 - 22 Intestinal cells 28 - 32 Intestinal cells UNIVERSITY OF PRETORIA UNIVERSITEIT VAN PRETORIA YUNIBESITHI YA PRETORIA Faculty of Veterinary Science Acknowledgments: The authors are grateful for the following financial support: (i) RED MEAT RESEARCH & DEVELOPMENT SA; (ii) Cape Wools SA; (iii) THRIP (National Research Foundation, South Africa), (iv) BBSRC/DFID/Scottish Government [grant number BBH00940/1] Reference: Van Wyk, J.A., Mayhew, E., 2013. Onderstepoort J. Vet. Res., 80(1), Art. #539, 14 pages http:// dx.doi.org/10.4102/ojvr. v80i1.539; Van Wyk et al., 2004. Vet. Parasitol., 119, 277-306 Parasitic Nematode Infective Larva (L3) Identification Organograms – Small Ruminants + Cattle 1 2 J.A. van Wyk and E. Mayhew 1 Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, 0110 Onderstepoort, South Africa. <[email protected]> 2 Department for Education Innovation, University of Pretoria Head With sheath Without sheath Uneven tip Squared Chabertia ovina 28-32 Intestinal cells Oesophagostomum spp. Bullet-shaped Haemonchus contortus Rounded Bunostomum/Gaigeria sp. Trichostrongylus Teladorsagia spp. axei Intestinal Trichostrongylus spp. Trichostrongylus spp. (e.g. T.rugatus,T. falculatus) Trichostrongylus spp. (e.g. T. colubriformis) Cooperia spp. e.g. C.curticei Finer tip Cooperia oncophora Blunter tip 16 Intestinal cells Tail smooth Tail notched/digitate 8 Intestinal cells Nematodirus spp. (see Fig. 7) X: 2.5 ± F: 10% ± F: 0% F ³ 20% F: 50% ± X: 5.0 - 5.5 ± X: 3.4 - 4.4 ± F: 50% ± Oesophagus+ bulb Tail smooth Tail digitate X: 1.0 ± F: 0% ± X: 1.7 ± F: 0% F: 20 - 25% ± X: 1.5 ± F: 20 - 25% ± X: 2.4 ± F: 20% ± F: 25% ± X: 4.1 ± X: 1.0 - 1.2 ± F: 0% X: 5.7 - 9.0 ± F: 50% ± Oesophagus rhabditiform Free-living nematode (see Fig. 13) Oesophagus 40% of length Thin larva Strongyloides spp. ± 18-22 Intestinal cells Organogram for identifying L3 of small ruminants to genus level (species names are listed, but L3 can seldom be identified to that level) Organogram for identifying L3 of cattle to genus level (species names are listed, but L3 can seldom be identified to that level)

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Head

With sheath Without sheath

Uneven tipSquared

Chabertia ovina

Oesophagostomum radiatum

Bullet-shaped

Haemonchus placei

Rounded

Bunostomum phlebotomum

Nematodirus helvetianus

X: ± 3.4

F: ± 20%

F: 40 - 45%±

X: 5.4±

X: 2.4±

F: 50%±

Tail smooth

F: 25%±

X: 5.1±

16 Intestinal cells

Nematodirus battus

X: 1.0 - 1.2±

F: 0%

Trichostrongylus axei

X: 2.2±

F: 10%±

Blunter tip

Ostertagia ostertagi

X: 2.0±

F: 20%±

Cooperia spp. (e.g. C. pectinata)

Finer tip

X: 3.1±

F: 20%±

Blunter tip

Cooperia oncophora

X: 8.3±

F: 50%±

X: 5.2±

F: 50%±

Tail notched/digitate8 Intestinal cells

Very long larvaFiner tip

Oesophagus rhabditiform

Free-living nematode(see Fig.13)

Oesophagus 40% of lengthThin larva

Strongyloides spp.

±

18 - 22 Intestinal cells

28 - 32 Intestinal cells

U N I V E R S I T Y O F P R E T O R I AU N I V E R S I T E I T V A N P R E T O R I A

Y U N I B E S I T H I Y A P R E T O R I A

Faculty of Veterinary Science

Acknowledgments: The authors are grateful for the following financial support:

(i) RED MEAT RESEARCH & DEVELOPMENT SA; (ii) Cape Wools SA; (iii) THRIP (National Research Foundation, South Africa), (iv) BBSRC/DFID/Scottish Government [grant number BBH00940/1]

Reference: Van Wyk, J.A., Mayhew, E., 2013. Onderstepoort J. Vet. Res., 80(1), Art. #539, 14 pages http:// dx.doi.org/10.4102/ojvr. v80i1.539; Van Wyk et al., 2004. Vet. Parasitol., 119, 277-306

Parasitic Nematode Infective Larva (L3) Identification Organograms – Small Ruminants + Cattle1 2J.A. van Wyk and E. Mayhew

1Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, 0110 Onderstepoort, South Africa. <[email protected]>2 Department for Education Innovation, University of Pretoria

Head

With sheath Without sheath

Uneven tipSquared

Chabertia ovina

28-32 Intestinal cells

Oesophagostomum spp.

Bullet-shaped

Haemonchus contortus

Rounded

Bunostomum/Gaigeria sp.

Trichostrongylus Teladorsagia spp.

axei

Intestinal Trichostrongylus spp.

Trichostrongylus spp.(e.g. T.rugatus,T. falculatus)

Trichostrongylus spp. (e.g. T. colubriformis) Cooperia spp. e.g. C.curticei

Finer tip

Cooperia oncophora

Blunter tip

16 Intestinal cells

Tail smooth

Tail notched/digitate8 Intestinal cells

Nematodirus spp.(see Fig. 7)

X: 2.5±

F: 10%± F: 0% F ³ 20%

F: 50%±

X: 5.0 - 5.5±

X: 3.4 - 4.4±

F: 50%±

Oesophagus+ bulb

Tail smooth

Tail digitate

X: 1.0±

F: 0%±

X: 1.7±

F: 0%

F: 20 - 25%±

X: 1.5±

F: 20 - 25%±

X: 2.4±

F: 20%±

F: 25%±

X: 4.1±

X: 1.0 - 1.2±

F: 0%

X: 5.7 - 9.0±

F: 50%±

Oesophagus rhabditiform

Free-living nematode(see Fig. 13)

Oesophagus 40% of lengthThin larva

Strongyloides spp.

±

18-22 Intestinal cells

Organogram for identifying L3 of small ruminants to genus level (species names are listed, but L3 can seldom be identified to that level)

Organogram for identifying L3 of cattle to genus level (species names are listed, but L3 can seldom be identified to that level)