path bacteria 04

7/27/2019 Path Bacteria 04

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Bacterial DiseasesAbout 37 species pathogenic to fish,

isolated from artificial propagation, crowded & stress

transmitted by waterborne

Bacterial pathogenic of fish ; Vibrio anguillarum, ordalli,

salmonicida, alginolyticus, damsela, cholera, vulnificus,

salmonicida, Aeromonas hydrophila, Pasteurella piscicida,Providencia rettgeri, Edwardsiella tarda, E. ictaluri, Serratia

plymuthica, Yersinia ruckeri, Acinetobacter, P. anguilliseptica, P.

chlororaphis, P. fluoresecens, Flexibaacter psychrophilus, F.

columnaris, F. maritimus, Flavobacterium branchiophila,Sporocytophaga sp., Richettsiiales, Renibacterium

salmoninarum, Eubacterium tarantellus, Carnobacterium

pisciola, Vagoccocus salmoninarum


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Lactoccocus piscium, Staphylococcus sp., Streptoverticillium, C.

botulinum, M. marinum, fortuitum, chelonei, Nocardia saterioides,

seriolae.

1. Edwardsiella tarda (serotype A, B, C, D)

Motile at 37C, produce indole-tryptophan broth, methyl red

+, utilize tartrate, grows on Christensens citrate. Isolation-

lesion o internal organs on BHIA, TSA

Fish lethargic, swim erratic, small cutaneous lesion 3-5

mm d, internal light colored nodule on spleen, kidney,

liver.

On case ofE. ictaluri, (enteric septicemia of catfish/ESC),

hemorrhage mouth, lateral & ventral, fin, pale gill,

exopthalmia, open lesion on skull hole in the head dis.

Internal, petechiae internal organ, ascites, swelling kidney,

liver, spleen.


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2. Yersinia, (enteric redmouth disease)

Affect salmon, isolated kidney, TSA, BHIA 20-25C, 48 h

disease acute, peracute,chronic red of mouth + inflamation, erotion o jaw, palate

opercula, externals, dark body, hemorrhage fin, bilateral

exopthalmia, internally, petechiae hemorrhage in visceral,

musculature, control by vaccine

3. Vibrio

devastaing of marine fish, higly invasive, produce

septicemia, acute disease. External, erythema fin, mouth,

hemorrhaging vent, petechiae, necrotic lesion in

musculture. Internal, congestion in liver, spleen, swelling

kidney, internal organs necrotic, liquified


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4. Aeromonas (furunculosis)

waterborne disease,A.salmonicida survive in salt water,

growth between 18-25C, slow at 4C, absent at 37C pathogen to salmonid, - freshwater n marine fish, focal

necrosis, hemorrhaging in pelvic, fin, splenomegally,

congestion of kidney, have A layer, leucocidin, two

proteases, hemolysin, glycerophospholipid, cholesterolacyltransferase. Isolation, 24 h, 20-25C,A.salminicida +

A. hydrophilla grows on 37C.

5. Pasteurella

infect marine fish, acute, chronic, in higher water

>23C/poor water quality, dark body, hemorrhaging

opercula + fin, internal, lesion kidney, spleen, tubercle-

like composed bacteria, epithelial cell, fibroblast.


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5. Rickettsiales (chlamydiales-like)

aquatic animals mortality 90%, lowered hematocrit,

swollen kidney, enlarged spleen, mottled liver.

Undientified parasit by light microscopy + EM, not known

in freshwater

growth on cell culture, obligately intracellular parasite,

optimum 15-18C, inhibited by streptomycin, gentamycin,tertracycline, pleomorphic, coccoid / ring form, in pairs,

replicated in cytoplasmic inclusion

6. Clostridium

anaerobic, absence catalase, can isolated f intestinalcontent fish, culture at 25-30C, type B, E, F produced

toxin as low as at 3.3.C

type isolated from sediment of pond, earthen pond, anaerobic


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Toxin tested by ip injection of mice/fish with supernatant

tissue

7. Lactid Acid Bacteria Normal flora freshwater + marine, Lactobacillus piscicola-

new genus Carnobacterium piscicola, after spawning d t

stress-related. isolated on TSA, BHIA, lesion, kidney, 48

h, 25 C, other strain are Vagococcus salmoninarum, L.piscium pathogenic to fish.

8. Renibacterium salmoninarum (bacterial kidney dis)

strict requirement cysteine, proteolytic, aerobic, opt 15 C

(5-22 C), catalase + but not cytochrome oxidase, slowly

progress, chronic, exopthalmia, lesion eyes, swollen

abdomen, internal, white greys pustule in kidney.

obligate pathogen, vertical transmitted via ovum,


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9. Mycobacterium spp.

acid fast organism, slow developing chronic dis, slow

grow at 18-25 C, not grow at 37 C, lethargic, anorexia,

skin ulcer, internal, granulomatous, necrotic lesion in kidney,

liver, spleen.

10. Nocardia

N. seriolae, growth after 10 d at 25C. chronic dis highmortality, emaciation, inactivity, skin discoloration, tubercle in

body, gill-called gill tuberculsis. internal, nodule 4 mm

spleen, swimbladder, kidney, liver, pericardium.

11. Flexibacter (F. psychrophilus, formerly Cytophaga psychrophila), F.

columnaris, F. maritimus gliding bacteria oder Cytophagales.

F. psychrophilus, bacterial cold water disease,internal,


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spleen + kidney, 3-5 d at 15-18C. F. columnaris, pathogen

to warm- cold water, yellowish lesion in gill + body. Isolated

from kidney, F. maritimus, eroded mouth, frayed fin, paleyellow, KCl + NaCl required for growth, opt grow is 30C

and range between 15-34C

path bacteria 04

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