pathology department - pathology services · 9. pull the specimen tube plunger , creating a vacuum...

Peterborough and Stamford Hospitals NHS Foundation Trust PATHOLOGY DEPARTMENT

SR-LF-BottleList Edition: 5 of 18

This is a controlled document, and valid only on the day of printing. For an updated copy please visit our website http://www.pch-pathlab.com/cms/

Peterborough and Stamford Hospitals NHS Foundation Trust Department of Pathology

Venepuncture and blood sample collection procedures

The following is a brief guide to the venepuncture procedures required for the collection of blood samples submitted to the Pathology Departments within the Peterborough and Stamford Hospitals NHS Foundation Trusts. All users should note, compliance with these procedures for phlebotomy/venepuncture is a minimum requirement for the utilisation of the Pathology service at PSHNFT. All users should ensure individuals who undertake venepuncture procedures are suitably trained and assessed for competency periodically. The Pathology department offers a phlebotomy training programme, for more information contact us on 01733 678430 or email [email protected] . The Pathology Department at PSHNFT utilises the Sarstedt blood collection system, below is a brief description of the procedures that should be followed when undertaking venepuncture:

1. Prior to undertaking the procedure, the phlebotomist should ensure their hands are thoroughly cleaned with an alcohol gel and/or soap and water

2. Ensure the patient is suitably prepared for the procedure (for example, where indicated check the patient has fasted, etc.).

3. Ask the patient to confirm their name and date of birth, and home address. Check these against the request form provided by the requesting clinician

4. Prepare the equipment; a needle, alcohol wipe, cotton wool and a tourniquet are required. Ensure you have all required bottles for the tests selected (see the Bottle List below)

5. Maintain sterility of the needle by keeping the cover on until required. Place the needle on the first sample bottle (ensure you have the correct draw order, see below), do not vacuum the first bottle (other bottles may be vacuumed at the discretion of the phlebotomist).

6. Inspect the patient’s arm and locate a suitable vein. Apply the tourniquet on the appropriate arm, causing the vein to swell.

7. Open the alcohol wipe and clean the area of venepuncture 8. Remove the cover of the needle (taking care to maintain sterility) and, ensuring the bevel of

the needle is uppermost, insert the needle into the vein 9. Pull the specimen tube plunger , creating a vacuum in the bottle and drawing blood into the

tube 10. Collect all tubes required in the correct order. 11. Remove pressure from the tourniquet 12. Place the cotton wool over the end of the needle and draw out of the arm. Ask the patient to

apply pressure to the cotton wool with the tourniquet. 13. Label the specimens immediately at the patient’s bedside with the required details (patient’s

full name, date of birth, patient number if available, date and time of collection and location). For specimens for transfusion requests, in the absence of a patient number the sample should be labelled with the patient address.

14. Specimens taken from ‘high risk’ patients (e.g. patients with blood borne virus infections such as HIV, Hepatitis B or C), specimens should be labelled with yellow high risk stickers.

15. Apply a plaster, or clean cotton wool and micropore tape, to the site of venepuncture 16. Ensure all waste is appropriately disposed, needles should be disposed in a biological sharps

bucket 17. Send the specimens with the corresponding request form(s) in the appropriate sample bag

compartments to the Pathology laboratory 18. The phlebotomist should clean their hands following the procedure

For Spillage advice: Contact the laboratory for advice in cases of specimen spillages and refer to Standard Operating Procedures SR-LP-006 spillage/cleaning

mailto:[email protected]




The following list details the bottle requirements for the profile of tests provided by the Pathology department at Peterborough City Hospital. Specimen bottles In most cases, a single specimen bottle is sufficient for each department, unless the bottle list indicates a separate sample is required, in which case an additional specimen must be taken. Please note, separate bottles are required for each department, i.e. Clinical Biochemistry, Immunology, Microbiology, etc. The volume of blood required varies depending upon the bottle type and tests required. For example:

Green coagulation bottles should be filled to the line indicated

Purple ESR (Stamford Hospital only) bottles should be filled to the stated line. For the majority of other tests, it is recommended that bottles are half-filled at a minimum. For further advice on individual specimen volumes, please contact the individual laboratories concerned. Specimens should be collected in the order listed below. Failure to follow this order may result in the invalidation of a number of assays and unnecessary repeat venepunture.

Draw order

Tube colour Picture Contents Key Uses

1st Brown

Clotted serum, gel barrier

Clinical Biochemistry, Immunology, some Microbiology

2nd

Green

Citrate Coagulation

3rd

Orange

Lithium heparin

Clinical Biochemistry

4th Purple

Citrate ESR STAMFORD HOSPIAL ONLY

5th Red (3.4.ml,

small)

EDTA Haematology (FBC, etc), HbA1c, PTH, ammonia

6th Red (7.5ml,

large)

EDTA Cross match, group and save, PCR tests

7th Yellow

Fluoride, EDTA

Glucose, lactate

It is essential to gently and thoroughly MIX all anticoagulant samples by gently tilting the bottle three or four times. Mixing immediately after venepuncture is particularly important for the EDTA (red) bottles. When obtaining blood for Coagulation studies or ESR ensure that the bottle is filled to the line.




SPECIMEN BOTTLE LIST

(i)TEST REQUESTED (ii)BOTTLE (iii) SPECIAL REQUESTS (iv) DEPARTMENT

ACE BROWN SEND AWAY

ACETYLCHOLINE RECEPTOR AB

BROWN SEND AWAY + IMMUNOLOGY

ACTH Transfusion Bottle

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. Must receive sample on ice within 10 minutes of venepuncture.

Chemistry SEND AWAY

ACYL CARNITINE (Total Carnitine)

GUTHRIE CARD IMMUNOLOGY

AFP(Alpha Feto Protein) BROWN CHEMISTRY

ALBUMIN (ALB) BROWN CHEMISTRY

ALCOHOL (Ethanol)(C2H5OH) Blood – YELLOW Urine - Universal

CHEMISTRY

ALDOSTERONE 2 x RED 1 x BROWN

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. Must be taken in conjunction with Renin and U/E.

SEND AWAY

ALKALINE PHOSPHATASE (ALP)

BROWN CHEMISTRY

ALLERGY TEST (Rast/specific IgE)

BROWN IMMUNOLOGY

ALP BROWN CHEMISTRY

ALP ISOENZYMES BROWN CHEMISTRY

ALPHA 1 ANTITRYPSIN (AAT) BROWN CHEMISTRY

ALPHA FETA PROTEIN(AFP) BROWN CHEMISTRY

ALT (Alanine Aminotransferase)

BROWN CHEMISTRY

ALUMINIUM WHITE (Plain) BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE.

SEND AWAY

AMINO ACID (BLOOD) ORANGE

Preferred Or BROWN

SEPARATE SAMPLE SEND AWAY

AMINO ACID (URINE) UNIVERSAL SAMPLE – URINE (minimum of 10 mL)

SEND AWAY




AMIODARONE BROWN SEPARATE SAMPLE SEND AWAY

AMMONIA (NH4) EDTA-K (RED), NOT KE-EDTA

MUST BE RECEIVED ON ICE ASAP

CHEMISTRY


AMYLASE BROWN CHEMISTRY

ANA (ANF) (Antinuclear Antibodies)

BROWN IMMUNOLOGY

ANCA(Antineutrophil cytoplasmic abs)

BROWN IMMUNOLOGY

ANDROSTENEDIONE BROWN SEPARATE SAMPLE SEND AWAY

ANTE NATAL TESTING Large RED HANDWRITTEN LABEL ONLY TRANSFUSION

ANTIBODY IDENTIFICATION 2 Large RED HANDWRITTEN LABEL ONLY TRANSFUSION

ANTI-EPIDERMAL AB (Skin) BROWN IMMUNOLOGY

ANTI-NUCLEAR AB’S BROWN IMMUNOLOGY

ANTI-SPERM AB’S BROWN IMMUNOLOGY

ANTITHROMBIN 111 (AT111) 5 GREEN FULL TO LINE HAEMATOLOGY

ARSENIC Blood -Large RED Urine - Universal


ASO (Anti Streptolysin Titre) BROWN MICROBIOLOGY

ASPERGILLUS BROWN MICROBIOLOGY

AST (Aspartate Amino Transferase)

BROWN CHEMISTRY

AUSTRALIAN ANTIGEN (Hep B)

BROWN MICROBIOLOGY

AUTOANTIBODIES BROWN Please request specific antibodies

IMMUNOLOGY

AVIAN PPT ANTIBODIES BROWN MICROBIOLOGY

B12 & FOLATE BROWN CHEMISTRY

BETA(β) 2 MICROGLOBULIN BROWN CHEMISTRY

BETA(β)-

HYDROXYBUTYRATE ORANGE

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. Glucose result is required for interpretation.

SEND AWAY

β-HCG (HCGP) BROWN ECTOPICS/URGENT CHEMISTRY




β-HCG (HCGS) BROWN TUMOUR MARKER CHEMISTRY

BENCE JONES PROTEIN URINE URINE< 8 Hrs Old CHEMISTRY


24hr BENCE JONES PROTEIN

Plain 24hr COLLECTION

MUST BE RECEIVED IN LAB ASAP.

CHEMISTRY

BICARBONATE BROWN CHEMISTRY

BILE ACID BROWN CHEMISTRY

BILIRUBIN(Split, Conjugated, Total)

BROWN CHEMISTRY

BIOTINIDASE ORANGE

SEPARATE SAMPLE MIN. SAMPLE VOLUME. 0.5 ML

SEND AWAY

BLOOD PORPHYRIN RED

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. SEPARATE SAMPLE KEEP SAMPLE IN DARK

SEND AWAY

BLOOD GROUP Large RED ADDRESS SAMPLE TRANSFUSION

BRUCELLA BROWN MICROBIOLOGY

C1 ESTERASE INHBITOR Functional

BROWN 1 EDTA + 1 BROWN

Antigenic only Please contact Immunology in advance (Send away Test)

IMMUNOLOGY

C3/C4 (Complement)

BROWN

CHEMISTRY

C3/C4 (Functional) 1BROWN 1 EDTA

Special sampling requirement Contact immunology ext 8454/8461 prior to taking blood

IMMUNOLOGY (SEND AWAY)

CA 125 BROWN CHEMISTRY

CA 15.3 BROWN SEPARATE SAMPLE SEND AWAY

CA 19.9 BROWN CHEMISTRY

CADMIUM Blood -Small RED Urine- Universal

SEPARATE SAMPLE Include an EMPTY Red or Universal of the same batch number.

SEND AWAY




CAERULOPLASMIN BROWN CHEMISTRY


CALCITONIN BROWN

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. SEPARATE SAMPLE REQUIRED. Must receive sample on ice within 30 mins of venepuncture.

SEND AWAY

CALCIUM

Blood – BROWN Urine – Universal (Spot) or plain 24hr collection.

CHEMISTRY

CALCULI (Stones) Universal SEND AWAY

CARBAMAZAPINE BROWN CHEMISTRY

CARBOHYDRATE DEFICIENT TRANSFERRIN (CDT)

BROWN SEPARATE SAMPLE SEND AWAY

CARBON MONOXIDE (Carboxyhaemoglobin)

RED CHEMISTRY

CARDIAC ENZYMES (CE / CPK)

BROWN CHEMISTRY

CARDIOLIPIN AB’S BROWN IMMUNOLOGY

CATECHOLAMINES (URINE) SPOT - Universal 24hr Collection - see Urine Metanephrines.

SEND AWAY

CD3, CD4 Small RED (ii) HAEMATOLOGY

CEA(Carcinoembryonic Antigen)

BROWN CHEMISTRY

CHLORIDE BROWN CHEMISTRY

CHOLESTEROL BROWN CHEMISTRY

CHOLINESTERASE 2 x RED SEPARATE SAMPLE SEND AWAY

CHROMOGRANIN A 1 x RED 1 x BROWN

INCLUDED IN GUT HORMONE PROFILE. BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. SEPARATE SAMPLE. Must receive samples within 15 minutes of venepuncture.

SEND AWAY

CHROMOGRANIN B 1 x RED 1 x BROWN

INCLUDED IN GUT HORMONE PROFILE. BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. SEPARATE SAMPLE. Must receive samples within 15 minutes of venepuncture.

SEND AWAY

CHROMOSOMES Small RED & ORANGE

SEPARATE SAMPLE. (iii) NOT FRIDAYS

SEND AWAY




CLEXANE GREEN FILL TO LINE HAEMATOLOGY

CLOTTING SCREEN GREEN FILL TO LINE HAEMATOLOGY


COAGULATION SCREEN GREEN FILL TO LINE HAEMATOLOGY

COLD AGGLUTININS Large RED KEEP WARM – TAKE STRAIGHT TO TRANSFUSION

TRANSFUSION

COPPER Blood – BROWN Urine - Universal


24hr URINE COPPER BLEEP 1150 or PHONE 678455.

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE.

SEND AWAY

CORTISOL BROWN CHEMISTRY

24hr URINE CORTISOL Plain 24hr Collection

SEND AWAY

COELIAC SCREEN (TTG) BROWN IMMUNOLOGY

C-PEPTIDE BROWN CHEMISTRY

C-REACTIVE PROTEIN (CRP) BROWN CHEMISTRY

CREATININE BROWN CHEMISTRY

CREATININE CLEARANCE BROWN & URINE

24 HOUR URINE (Blood sample can be sent up to 48 hours before or after urine collection)

CHEMISTRY

CROSS MATCH Large RED ADDRESS SAMPLE TRANSFUSION

CRYOGLOBULIN 2 x RED 1 x WHITE

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. Pre-heated tubes MUST be used.

CHEMISTRY

CYCLOSPORIN Small RED SEPARATE SAMPLE *NOT FRIDAY*

SEND AWAY

CYSTINE (URINE) SPOT Universal or 24hr Collection - Plain

SEND AWAY

CYSTIC FORBROSIS SCREEN (∆508)

Large RED SEPARATE SAMPLE SEND AWAY

7-DEHYDROCHOLESTEROL BROWN SEPARATE SAMPLE SEND AWAY

D-DIMER GREEN FILL TO LINE HAEMATOLOGY

DHEAS BROWN SEPARATE SAMPLE SEND AWAY

DIGOXIN (6 HOURS POST DOSE)

BROWN STATE ON REQ DOSE TIME CHEMISTRY

DIRECT COOMBS TEST(DCT)

Large RED TRANSFUSION




DRUGS OF ABUSE 20ml URINE SEND AWAY

E2 (Oestradiol) BROWN CHEMISTRY


EPO (Erythropoietin) BROWN SEPARATE SAMPLE SEND AWAY

EPS (PROTEIN ELECTROPHORESIS)

BROWN CHEMISTRY

ENA (EXTRACTABLE NUCLEAR ANTIGENS)

BROWN IMMUNOLOGY

ENDOMYSAL BROWN IMMUNOLOGY

ESR

Small EDTA 3.4ml

Stamford Hospital require Purple citrate bottle filled to the line

HAEMATOLOGY

ETHOSUXIMIDE BROWN SEPARATE SAMPLE SEND AWAY

FACTOR 8, 9,11,12 4 GREEN FILL TO LINE HAEMATOLOGY

FAECAL ELASTASE (i) BLUE TOPPED FAECAL BOTTLE

SEND AWAY

FAECAL OCCULT BLOOD 3 x Hemoccult Assay Cards

ENSURE ALL CARDS ARE LABELLED WITH PATIENT ID.

CHEMISTRY

FAECAL PORPHYRIN FAECAL BOTTLE BLUE

SAMPLE MUST BE KEPT IN DARK

SEND AWAY

FERRITIN BROWN CHEMISTRY

FLECANIDE BROWN SEPARATE SAMPLE SEND AWAY

FOLATE BROWN CHEMISTRY

FOLLICLE STIM HORMONE (FSH / LF)

BROWN CHEMISTRY

FRAGILE X ORANGE & Small RED


FREE FATTY ACIDS (FFA) ORANGE

SEPARATE SAMPLE. Sample must be separated within 1 hour of venepuncture and a glucose concentration is required for result interpretation.

SEND AWAY

FREE & TOTAL PSA BROWN SEPARATE SAMPLE SEND AWAY

FREE T3 (FT3) BROWN CHEMISTRY

FREE T4 (FT4) BROWN CHEMISTRY

FRUCTOSAMINE BROWN SEPARATE SAMPLE SEND AWAY




FULL BLOOD COUNT (FBC) Small RED HAEMATOLOGY

FUNCTIONAL ANTIBODIES BROWN STATE TYPE REQUIRED IMMUNOLOGY


G6 PD SCREEN Small RED HAEMATOLOGY

GALACTOSE-1-PHOSPHATE URIDYL TRANSFERATE

GUTHRIE CARD SEND AWAY

GASTRIC PARIETAL AB’S (PA Antibodies)

BROWN IMMUNOLOGY

GASTRIN 1 x RED 1 x BROWN

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. SEPARATE SAMPLE.

SEND AWAY

GAULT COCKCROFT (GFRM)(GFRW)

BROWN HEIGHT & WEIGHT ON FORM CHEMISTRY

GENETIC SCREENING RED SEPARATE SAMPLE SEND AWAY

GENTAMICIN BROWN CHEMISTRY

GGT (Gamma GT) BROWN CHEMISTRY

GLUCOSE YELLOW ASK IF FASTING CHEMISTRY

GLYCINE CSF – Universal Blood - ORANGE

SEPARATE SAMPLE MIN. VOLUME 0.2 ML. Must receive a blood and CSF sample.

SEND AWAY

GLYCOSYLATED HAEMOGLOBIN (HbA1c)

Small RED SEPARATE SAMPLE CHEMISTRY

GLYCOSAMINOGLYCANS / MUCOPOLYSACCHARIDES (URINE)

URINE 20 mls MIN VOLUME – 10 MLS SEND AWAY

GROUP & SAVE Large RED (handwritten)

ADDRESS SAMPLE TRANSFUSION

GROWTH HORMONE (GH) BROWN CHEMISTRY

URINE GROWTH HOROMONE

Plain 24hr Collection

SEND AWAY

GTT (Glucose tolerance test) PHONE 678468 FOR INFO TO MAKE APPOINTMENT

CHEMISTRY

GUT HORMONE 1 x RED

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. SEPARATE SAMPLE. Must receive samples within 15 minutes of venepuncture.

SEND AWAY

HAPTOGLOBINS BROWN HAEMATOLOGY

HAEMACHROMATOSIS LARGE RED SEPARATE SAMPLE SEND AWAY

HAEMOGLOBIN ELECTROPHORESIS

Small RED HAEMATOLOGY




HBA2 Small RED HAEMATOLOGY

HDL/LDL (Lipid profile) BROWN ASK IF FASTING CHEMISTRY


HEPARIN RATIO GREEN FILL TO LINE HAEMATOLOGY

HEPATITIS SEROLOGY (A,B,C&E)

BROWN

SEPARATE SAMPLE 2 Bottles for Full Hepatitis Screen

MICROBIOLOGY

HIV BROWN MICROBIOLOGY

HLA B27 Small RED NOT FRIDAY HAEMATOLOGY

HOMOCYSTEINE ORANGE SEPARATE SAMPLE SAMPLE TO CHEM LAB

SEND AWAY

HOMOCYSTINE ORANGE SEPARATE SAMPLE SEND AWAY

URINE 5-HYDROXYINDOLACETIC ACID (5-HIAA)

Acidified 24hr Collection

Contact 678468 for bottles CHEMISTRY

17-HYDROXYPROGESTERONE (17 OHP)


SALIVARY 17-HYDROXYPROGESTERONE (17-OHP)

4 x UNIVERSAL

SEPARATE SALIVA COLLECTION IN EACH UNIVERSAL

SEND AWAY

IGE (TOTAL) BROWN IMMUNOLOGY

IMMUNOGLOBULIN (EPS) BROWN CHEMISTRY

IMMUNOGLOBULINS A/G/M (igA/G/M)

BROWN CHEMISTRY

IMMUNOGLOBULIN D (IgD) BROWN SEPARATE SAMPLE SEND AWAY

IMMUNOGLOBULIN E (IgE) BROWN IMMUNOLOGY

INR GREEN FILL TO LINE HAEMATOLOGY

INSULIN ORANGE or BROWN

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. SEPARATE SAMPLE – Must be received on ice ASAP.

CHEMISTRY SEND AWAY

INSULIN LIKE GROWTH FACTOR 1 (IGF-1)


INTRINSIC FACTOR (PA ASSOCIATE ANTIBODIES)

BROWN IMMUNOLOGY

IRON (OVERDOSE) BROWN BLEEP 1150 or PHONE 678455 for info.

CHEMISTRY




KLEIHAUER ADHOC Large RED & Small RED

ADDRESS ON SAMPLE TRANSFUSION

KLEIHAUER MOTHER Large RED & Small RED

ADDRESS ON SAMPLE TRANSFUSION


BABY Large RED ADDRESS ON SAMPLE TRANSFUSION

LAC SCREEN 5 GREEN FILL TO LINE HAEMATOLOGY

LACTATE YELLOW BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE.

CHEMISTRY

LACTATE DEHYDROGENASE (LDH)

BROWN (Fluid LDH not available) CHEMISTRY

LACTATE PYRUVATE CONTACT CHEMISTRY

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE.

SEND AWAY

LAMOTRIGENE BROWN SEPARATE SAMPLE SEND AWAY

LEAD Small RED

SEPARATE SAMPLE & Small EMPTY RED of same batch

SEND AWAY

LEPTOSPRIOSIS BROWN MICROBIOLOGY

LEUTINISING HORMONE (LH / LF)

BROWN CHEMISTRY

LIPID PROFILE BROWN ASK IF FASTING CHEMISTRY

LIPOPROTEIN PROFILE + STRIP

BROWN SEPARATE SAMPLE CHEMISTRY

LITHIUM BROWN SEPARATE SAMPLE CHEMISTRY

LIVER FUNCTION TESTS (LFT)

BROWN CHEMISTRY

LUPUS ANTICOAGULANT 5 GREEN FILL TO LINE HAEMATOLOGY

LYSIS TEST Small RED HAEMATOLOGY

LYMES DISEASE BROWN MICROBIOLOGY

M2 (Mitochondrial antibodies) BROWN IMMUNOLOGY

MAGNESIUM

Blood – BROWN Urine – Universal (Spot) or Plain 24hr Collection.

CHEMISTRY

MALARIA Small RED LABEL URGENT HAEMATOLOGY

MERCURY (URNIE) Spot (Universal) or 24hr Collection

CONTACT LAB SEND AWAY




METFORMIN (GLUCOPHAGE)


MERCURY (blood) ORANGE SEPARATE SAMPLE SEND AWAY


METABOLIC SCREEN URINE (20 ml) SEND AWAY

METANEPHRINES (URINE) Acidified 24hr Collection

CONTACT 678468 for bottles. CHEMISTRY

METHOTREXATE ORANGE, BROWN or RED


MICROALBUMIN (MALB/ACR) URINE- Universal CHEMISTRY

MITOCHONDRIAL AB’S BROWN SEPARATE SAMPLE IMMUNOLOGY

MORPHINE ORANGE SEPARATE SAMPLE SEND AWAY

MYCOPHENOLATE RED

SEPARATE SAMPLE Sample should be PRE-dose. Last dose and time required for interpretation.

SEND AWAY

NUCLEAR AB’S BROWN IMMUNOLOGY

N-terminal Pro-BNP (NT-ProBNP)

BROWN CHEMISTRY

OLIGOCLONAL BANDS BROWN & CSF SAMPLE

SEND AWAY

ORGANIC ACIDS URINE 10 mL minimum volume SEND AWAY

OROTIC ACID URINE 5 mL minimum volume SEND AWAY

OSMOLALITY SERUM BROWN CHEMISTRY

OSMOLALITY URINE URINE Random Sample CHEMISTRY

OXALATE (URINE) Plain 24hr Collection

SEND AWAY

PARACETAMOL BROWN CHEMISTRY

PARAQUAT SCREEN (Urine of Gastric Aspirate)

UNIVERSAL CHEMISTRY

PARAQUAT QUANTIFICATION

BROWN Only performed if Paraquat Screen is positive.

SEND AWAY

PARATHORMONE (PTH) YELLOW CHEMISTRY

PARIETAL CELL AB’S BROWN IMMUNOLOGY

PAUL BUNNELL Small RED HAEMATOLOGY




PHENOBARBITONE BROWN CHEMISTRY

PHENYTOIN (EPANUTIN) BROWN CHEMISTRY


PHOSPHATE (Inorganic Phosphate / IPH)

Blood - BROWN Urine – Universal (Spot) or Plain 24hr Collection

CHEMISTRY

PHYTANIC ACID RED SEPARATE SAMPLE SEND AWAY

PLASMA VISCOSITY Small RED HAEMATOLOGY SEND AWAY

PLATELET IMMUNOLOGY CONTACT LAB 8451 TRANSFUSION

PORPHYRIN SCREEN URINE – SEND ASAP

KEEP SAMPLE IN DARK CHEMISTRY

POTASSIUM

Blood – BROWN Urine – Universal (Spot) or Plain 24hr Collection

Do not refridgerate blood samples BEFORE centrifugation.

CHEMISTRY

PURINE SCREEN (Urine) Universal SEND AWAY

PREGNANCY TEST (URINE βhCG)

Universal CHEMISTRY

PROGESTERONE BROWN STATE DAY OF CYCLE CHEMISTRY

PRO-INSULIN ORANGE or BROWN


PROLACTIN BROWN CHEMISTRY

PROSTATE SPECIFIC ANTIGEN (PSA)

BROWN CHEMISTRY

PROTEIN S & C 5 GREEN FILL TO LINE HAEMATOLOGY

PROTEIN (TOTAL)


CHEMISTRY

PROTHROMBIN GREEN FILL TO LINE HAEMATOLOGY

PSEUDOCHOLINESTERASE BROWN SEPARATE SAMPLE SEND AWAY

PT (Liver Screen) GREEN FILL TO LINE HAEMATOLOGY

PYRIMIDINE SCREEN (Urine) UNIVERSAL SEND AWAY

PYRUVATE KINASE Small RED HAEMATOLOGY

QUANTIFERON GOLD

DISCUSS WITH IMMUNOLOGY EXT 8454 / 8461 NOT ON FRIDAYS

IMMUNOLOGY




RA LATEX/FACTOR (Rheumatoid Factor)

BROWN CHEMISTRY


RAST BROWN SPECIFY ALLERGY IMMUNOLOGY

RED CELL TPMT RED LARGE SEPARATE SAMPLE SEND AWAY

RED CELL TRANSKETOLASE PHONE 678468 FOR INFO SEND AWAY

RENAL PROFILE LITHIUM Gel Bottle

CHEMISTRY

RENIN 2 x RED 1 x BROWN

BLEEP 1150 or PHONE 678455 BEFORE TAKING SAMPLE. Must be taken in conjunction with Aldosterone and U/E.

SEND AWAY

RESPIRATORY SCREEN BROWN MICROBIOLOGY

RICOFF 4 GREEN FILL TO LINE HAEMATOLOGY

RUBELLA BROWN SEPARATE SAMPLE MICROBIOLOGY

SALICYLATE BROWN CHEMISTRY

SELENIUM BROWN or ORANGE


SERUM FREE LIGHT CHAINS BROWN CHEMISTRY

SEX HORMONE BINDING GLOBULIN (SHBG)


SICKLE CELL TEST Small RED HAEMATOLOGY

SIROLIMUS/SEROLIMUS LARGE RED NOT FRIDAY. Sample should be taken at dosage trough.

SEND AWAY

SKIN ANTIBODIES BROWN IMMUNOLOGY

SMOOTH MUSCLE AB’S BROWN IMMUNOLOGY

SODIUM


CHEMISTRY

SPECIFIC ANTIBODIES BROWN STATE TYPE IMMUNOLOGY

SPERM AB’S BROWN IMMUNOLOGY

STEROID PROFILE (URINE) RANDOM URINE 20 ml SEND AWAY




STS(Serology for Syphilis) (iii) BROWN

(iv) MICROBIOLOGY

SYNACTHEN TEST BROWN SEE CORTISOL CHEMISTRY


TACROLIMUS (FK508) Small RED SEPARATE SAMPLE SEND AWAY

TAU PROTEIN (b TRANSFERRIN)

CSF - Universal PHONE 8468 FOR INFO SEND AWAY

TB SUB SETS Small RED NOT FRIDAY HAEMATOLOGY

TESTOSTERONE BROWN CHEMISTRY

THALASSAEMIA Small RED HAEMATOLOGY

THEOPHYLLINE (STATE DOSE)

BROWN CHEMISTRY

THROMBOTIC SCREEN 5 GREEN (v) HAEMATOLOGY

THYROGLOBULIN BROWN SEPARATE SAMPLE SEND AWAY

THYROXINE BINDING GLOBULIN


THYROID FUCTION TEST BROWN CHEMISTRY

THYROID PEROXIDASE (TPO)

BROWN CHEMISTRY

THYROID STIMULATING HORMONE (TSH)

BROWN CHEMISTRY

TORCH BROWN MICROBIOLOGY

TOXOCARA BROWN MICROBIOLOGY

TOXOPLASMOSIS BROWN MICROBIOLOGY

TPHA-see STS BROWN MICROBIOLOGY

TRANSFERRIN ISOFORMS BROWN SEPARATE SAMPLE SEND AWAY

TRANSFUSION REACTION

PHONE LAB 8451

TRANSFUSION

TREPONEMAL- see STS BROWN

MICROBIOLOGY

TRIGLYCERIDES BROWN CHEMISTRY

TROPONIN T BROWN CHEMISTRY




TRYPTOPHAN ORANGE SEND AWAY

TSH RECEPTOR ANTIBODIES

BROWN IMMUNOLOGY SEND AWAY


TTG (Anti Tissue Transglutaminase Antibodies)

BROWN IMMUNOLOGY

U+E (Creatinine + Electrolytes) BROWN Do not refridgerate sample BEFORE centrifugation.

CHEMISTRY

UREA


CHEMISTRY

URIC ACID


CHEMISTRY

URINE 24 HOURS(All Types) 24 HR CONTAINER

PHONE 678468 FOR INFO CHEMISTRY

URINE REDUCING SUBSTANCES

UNIVERSAL Must be received in lab within 2 hours of collection.

CHEMISTRY

VALPROATE (Epilim) BROWN SEPARATE SAMPLE SEND AWAY

VANCOMYCIN BROWN CHEMISTRY

VDRL- see STS BROWN MICROBIOLOGY

VERY LONG CHAIN FATTY ACIDS

Small RED or ORANGE


VIRAL LOADS EDTA MICROBIOLOGY

VIRAL SEROLOGY BROWN MICROBIOLOGY

VISCOSITY Small RED HAEMATOLOGY SEND AWAY

VITAMIN A & E BROWN SEPARATE SAMPLE SEND AWAY

KEEP IN THE DARK

VITAMIN D BROWN CHEMISTRY

VON WILLI BRANDS 4 GREEN FILL TO LINE HAEMATOLOGY

WEIL TEST -FELIX TEST (Typhus)

BROWN MICROBIOLOGY

WHITE CELL ENZYMES RED PHONE 678468 **NOT ON FRIDAY**

SEND AWAY

WR- see STS BROWN MICROBIOLOGY




YERSINIA SEROLOGY BROWN MICROBIOLOGY

ZINC


SEPARATE SAMPLE (BLOOD) SEND AWAY

SPECIAL AND NEW ADDED TESTS

A29 - 4 GREEN - IMMUNOLOGY

HLAB5 – 4 GREEN - IMMUNOLOGY

PCR tests – DNA, RNA, Genotyping or Viral Loads use a RED EDTA bottle as well as a BROWN bottle

ALL TRANSFUSION SPECIMENS MUST BE HANDWRITTEN ONLY – SAMPLES WITH ADDRESSOGRAPH LABELS WILL BE REJECTED Confusing requests

Request Test required Bottle

SERUM PROTEIN TPR BROWN

SERUM ELECTROPHORESIS EPS BROWN

HB ELECTROPHORESIS HBELEC (HAEM) RED

GLYC HB HBA1C RED

THALASSEMIA TRAIT HBA2 (HAEM) RED

PROTEIN AND STRIP EPS BROWN

IMMUNOGLOBULINS IGs BROWN

IMMUNOELECTROPHORESIS EPS BROWN

MYELOMA SCREEN EPS BROWN

MYELOMA URINE SCREEN BJP URINE – FRESH TO LAB

PARAPROTEINS EPS BROWN

IGM, IGA, IGG IGs BROWN

IGE IMMUNOLOGY (Total IGE Discuss with IMMUNOLOGY EXT 8454 / 8461)

BROWN

IGG SUBCLASSES SEND AWAY BROWN




MISCELANEOUS TESTS BOTTLE DEPARMENT

BONE PROFILE BROWN CHEMISTRY

T SPOT Superseded by Quantiferon Discuss with Immunology ext 8454/8461)

IMMUNOLOGY

FOQ SMALL RED HAEMATOLOGY

GENTAMYCIN BROWN / SEP SAMPLE CHEMISTRY

VANCAMYCIN BROWN / SEP SAMPLE CHEMISTRY

pathology department - pathology services · 9. pull the specimen tube plunger , creating a vacuum...

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