patterns of inheritance. mid-1800s austrian monk, gregor mendel used pea plants to study how traits...
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Patterns of Inheritance
Intro To Genetics
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Gregor Mendel • Mid-1800s• Austrian Monk, Gregor Mendel• Used pea plants to study how traits are
passed from one generation to the next. • “Basic principles of Heredity”
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Mendelian Genetics: • For his experiments on inherited traits,
Mendel used pea plants • Because they could produce purebred
individuals (genetically identical)• How? Self-pollination • Once he produced Pure-bred strains, he
began to interbreed them in a controlled environment.
• Mendel crossed pure-bred GREEN-POD (dominant) plants with pure-bred YELLOW-POD (recessive) plants.
• The results are shown below:
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Why did all the offspring come out green, if one of it’s parents were yellow?
• Pure-bred= Homozygous= Parent generation
Parent 1- Homozygous Green (GG)Parent 2- Homozygous Yellow (gg)
• When they were interbred, each parent contributed one allele to the offspring (First Generation)= one G and one g!
Offspring 1- Heterozygous offspring (Gg)
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SEGREGATION Mendel didn’t just stop after crossing the parent plants,
he had another question… Had the recessive alleles simply disappeared,
or were they still present in the new plants?
P- Parent Generation F1- First (filial) Generation F2- Second (filial) Generation
• The F2 cross shows the recessive alleles reappeared in the second generation. HOW?
• The Reappearance indicated that, at some point, the allele for shortness had separated or segregated from the allele for tallness. HOW?
The alleles for tallness and shortness in the F1 plants must have segregated from each other during the formation of the SEX CELLS, or GAMETES.
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FORMATION OF GAMETESP Generation= Tall plant (TT) X short plant (tt)
F1 Generation=All tall plants with genotype (Tt)
F1 (Tt) X F1 (Tt) F2 Generation= 1 Tall plant (TT), 2 Tall plants with (Tt), 1 SHORT plant with (tt)
During GAMETE FORMATION, the alleles for each gene segregate from each other, so that each gamete carries only one allele for each gene.
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SUMMARY OF MENDEL’S PRINCIPLES
Mendel’s Principles of Heredity, observed through Patterns of Inheritance, form the BASIS OF MODERN GENETICS.
1. The Inheritance of biological characteristics is determined by individual units called GENES, which are passed from parent to offspring.
2. Where two or more form (alleles) of the gene for a single trait exist, some alleles may be DOMINANT and others may be RECESSIVE.
3. In most sexually reproducing organisms, each adult has TWO COPIES of each gene—one from each parent. These genes SEGREGATE from each other when gametes are formed.
4. Alleles for different genes usually SEGREGATE INDEPENDENTLY of each other.
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Many Organisms, including humans, reproduce sexually. • They receive genes from both their parents • Both parents contribute genes for the same traits.
The Genes may be the same, or they may code for different forms of a trait.
Gene= heightTrait (allele)= short or tall
Alleles- are different forms of the gene for a specific trait.
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Genotype: • Complete set of genes carried by
an organism • Includes all the alleles that are
not expressed as well as those that are.
• Ex: Rabbit Fur genotype can include black and brown fur (Bb)
Phenotype:• Set of traits that an
organism displays• What you actually express
physically• Ex: Rabbit Fur Phenotype
for (Bb) would be black fur.
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Dominant And Recessive Traits
Dominant Traits When an organism has two different alleles for a
trait, the dominant allele is expressed (phenotype).
Uppercase letters EX: Black Fur in rabbits BB (two dominant
alleles)
Recessive Traits Are expressed only when no dominant alleles
are present. Lowercase letters
EX: Brown fur in rabbits bb (two recessive alleles)
Organism that receives two identical alleles for a characteristic shows that
characteristic (phenotype).
Trait Dominant Recessive
Freckles Present Absent
Hairline Widow’s peak
Straight
Earlobe Free Attached
Ability to taste PTC (phenylthiocarbamide)
Tasting Nontasting
Homozygous- two dominant or two recessive
(BB or bb). Heterozygous- two different alleles for a trait
(Bb).
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Phenylthiocarbamide has the unusual property that it either tastes very bitter or is virtually tasteless, depending on the genetic makeup of the taster.
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PUNNETT SQUAREUseful for finding the PROBABILITY of a simple genetic cross. • Parent’s alleles are written across the top and side of the
square. • Combining these alleles give the POSSIBLE genotypes of the
offspring
PRACTICE: Create a Punnett Square in your notes:
1. Mom has Blue eyes (bb)2. Dad has dark brown eyes (Bb)
What is the possible genotypes and phenotypes of their unborn baby?
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MOM DAD
Monohybrid Cross(One-Factor Cross)
Dihybrid Cross (Two-Factor Cross)
Step 1: Write the Genotypes for the parents Ex: Pea Plant Height (Tt) and/or Color (Gg)
Tt and Tt TtGg and TtGg
Step 2: What alleles would be found in all possible gametes of the parents
Tt—T and tTt—T and t
TtGg—TG, Tg, tG, and tgTtGg—TG, Tg, tG, and tg
Step 3: Draw a table with enough squares for each pair of gametes from each parent
Step 4: Fill in the table by combining the gametes’ genotypes.
Step 5: Determine the genotype and phenotype of each offspring. Calculate the percentage or ratio of each.
T tTt
TG tG Tg tg
tG
TG
tg
Tg
TtGgTT
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Intermediate Traits
Incomplete Dominance- the result is a BLEND of the two forms of the trait
Ex: Flower color
Codominance- Condition in which both alleles are expressed in the same organism.
Ex: Chicken Feathers & ABO Blood group
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Multiple Alleles- although each organism has only two alleles for the trait, more than two possible alleles may exist in the population. Ex: Human Blood Types (A, B, AB, or O)
Polygenic Traits- are controlled by two or more genesEx: Height in humans
Blood Transfusions
Blood Type of Donor
Blood Type of Recipient
A B AB O
A YES X YES X
B X YES YES X
AB X X YES X
O YES YES YES YES
Intermediate Traits
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Sex-Linked Inheritance- Because X and Y chromosome determines sex, the genes located on them show a pattern of inheritance.
Sex-linked gene- gene located on a sex chromosome
• Genes on the Y chromosome are found ONLY in MALES and are passed directly from father to son.
• Genes located on the X chromosome are found in both sexes, but the fact that men have just one X chromosome leads to some interesting consequences.• Ex: three genes responsible for color vision, all
located on X chromosome • Males- defective allele for any of these genes results
in colorblindness • In order for a RECESSIVE allele to be EXPRESSED in
FEMALES, it must be present in TWO copies—one on each of the X chromosomes.
RECESSICE PHENOTYPES of a Sex-Linked genetic disorders tends to be much more COMMON among MALES than among females.
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PedigreesShows the presence or absence of a trait according to the relationships between parents, siblings, and
offspring.
• By analyzing a pedigree, we can often GUESS the genotypes of family members.• Based on a pedigree, you can often determine if an allele for a trait is dominant or
recessive, autosomal or sex-linked.
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Human Genetic Disorders“It runs in the family”
What does that really mean?
• Changes in a gene’s DNA sequence can change proteins by altering their amino acid sequences, which may directly affect one’s phenotype.
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Disorders Caused by individual Genes:
Sickle Cell Disease: o Disorder caused by a defective allele for beta-
globin, one of two polypeptides in hemoglobin. o The defective polypeptide makes HEMOGLOBIN less
soluble, causing them to STICK TOGETHER. o This causes the cell to get a SICKLE-SHAPE. o Because of its shape it gets stuck in the capillaries
Huntington’s Disease:o Caused by a dominant allele for a protein found in
brain cells. o The allele for this disease contains a long string of
bases in which the codon CAG (which codes for Glutamine) repeats over and over again.
o Symptoms include mental deterioration and uncontrollable movements
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Central Dogma of Biology
DNAmRNAprotein DNA TRANSCRIBES to mRNA
Process is called transcription
mRNA TRANSLATES to proteins Process is called translation mRNA actually makes amino acids, which come together
to make proteins
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Nucleic Acids
DNA Double strand Deoxyribose sugar A=T G=C
RNA Single Strand Ribose sugar A=U G=C Uracil is the nitrogenous base
used instead of THYMINE
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DNA Replication Simplified
Unzip parent DNAAdd nucleotides to the 2 template strands of DNA
DNA parent strand makes 2 daughter strands…one fast, smart daughter strand (leading) and one, slower, no-fast daughter strand (lagging)
Leading strand (runs 3’ to 5’) Lagging strand (runs 5’ to 3’)
Attach fragments on lagging strand
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DNA Polymerases Enzymes Make covalent bonds between nucleotides of the new strands Fast, accurate process
Error only one in a billion nucleotides
Brings over nucleotides to unzipped DNA strand and drops them off
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More Players in DNA Replication
DNA polymerase can only read a strand that is running 3-prime to 5-prime… DNA polymerase works non-stop
adding nucleotides onto the strand that runs in the 3’ to 5’ direction
Therefore, Only one strand is made by a smooth, and continuous process…
The other strand is put together in bits and pieces… Each little section of nucleotides is
called an “Okazaki Fragment” These are then “glued” together to
make one, continuous strand in the
end by another enzyme… DNA Ligase
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OKAZAKI FRAGMETS!!!are short, newly synthesized DNA fragments that are formed on the lagging template strand during DNA replication.
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Important Enzymes
DNA Helicaseunzips
DNA PolymeraseAdds nucleotides
DNA LigaseAttaches/glues okazaki
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DNAmRNAamino acids/polypeptide chain (Proteins)
DNA codes for an RNA strand The every 3 bases on the RNA strand
code for a specific amino acid CODON: three sequential bases that
code for a specific a.a. (20 a.a. total) Amino acid are strung together to
make a protein (primary structure) Change DNA will change RNA which
will change amino acids, which change protein
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Ala: Alanine Cys: Cysteine Asp: Aspartic acid Glu: Glutamic acidPhe: Phenylalanine Gly: Glycine His: Histidine Ile: Isoleucine Lys: Lysine Leu: Leucine Met: Methionine Asn: AsparaginePro: Proline Gln: Glutamine Arg: Arginine Ser: SerineThr: Threonine Val: Valine Trp: Tryptophane Tyr: Tyrosisne
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DNAmRNAProtein
Transcription Different form of the same message DNA makes single stranded RNA (U replaces T) RNA leaves nucleus
Translation Translate from nucleic acid language to amino acid language Uses codons, 3-base “word” that codes for specific a.a.
“code” for an amino acid Several codons make a “sentence” that translates to a
polypeptide (protein)
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The Genetic Code
Am. Biochemist Marshall Nirenberg began to crack the genetic code in the 1960s Built RNA model with Uracil, called poly U, conducted
experiments with it and figured out UUU coded for amino acid phenylalanine
Scientists used his procedures to figure out the other amino acids represented by codons
Stop codons: UAA, UGA, UAG SIGNAL END OF GENETIC MESSAGE
Start codon: AUG SIGNAL TO START TRANSLATING an RNA transcript
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Start Codons
UAAUGAUAG
Stop Codons
AUG
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Three Types of RNA
mRNAtRNArRNA
Three Types of RNA…
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Three Types of RNA… #1
mRNA (messanger RNA) RNA transcribed from DNA template RNA polymerase (enzyme) links RNA nucleotides together Modified in nucleus before if exits
RNA splicing: process in which Introns are removed and exons re joined together to make a continuous coding mRNA molecule
Introns Internal non-coding regions of DNA and mRNA Space fillers They are cut out of mRNA before it is allowed to leave the nucleus Process is called RNA splicing (processing)
Exons Coding region of DNA and mRNA that will be translated (Expressed) VERY important part of mRNA…it is carrying the message from DNA (def can’t cut this out)
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Three Types of RNA…#2
tRNA (transfer RNA) The interpreter Translate 3-letter base codes into
amino acids Carries anti-codon on one end
(three letters opposite of what is on mRNA)
Carries amino acid on other end Anti-codon recognizes codon and
attaches
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Three Types of RNA…#3
rRNA (ribosomal RNA) Found in ribosome Ribosome composed of 2 subunits:
Small subunit for mRNA to attach Large Subunit for two tRNAs to
attach “P” site: holds the tRNA carrying
the growing polypeptide chain “A” site: holds the tRNA that is
carrying the next a.a. to be added to the chain
When stop codon (UAA, UAG, UGA) is reached, translation ends and polypeptide is released from tRNA by hydrolysis
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