+NH

O

O

OH

NH

O

OO

HN

SO

NHHN

O

PCDA-ABA

PCDA-Biotin

Directed Self-assembly/ Emissive organic nanoparticles

Hyong-Jun Kim, Jiseok Lee, Tae-Hoon Kim, Taek Seung Lee, and Jinsang Kim “Highly Emissive Self-assembled Organic Nanoparticles having Dual Color Capacity for Targeted Immunofluorescence Labeling” Adv. Mater. Accepted.

PEGfilling

Biotinspotting

Avidinbinding

Nanoparticle binding

Biotin

PEG

Avidin

DBO-PCDA

Functional emissive organic nanoparticles for immunofluorescence labeling

CAREER: Self-signaling and Signal Amplifying Conjugated Polymer Biosensors and Sensor Arrays

Jinsang Kim, University of Michigan, DMR 0644864

CAREER: Self-signaling and Signal Amplifying Conjugated Polymer Biosensors and Sensor Arrays

Jinsang Kim, University of Michigan, DMR 0644864

• Research Highlight:

• Education Highlight:

Immunofluorescence labeling is a very important analytical technique for probing the structure of living cells. Such labeling is conventionally carried out by using fluorescent organic dyes. However, some drawbacks including a limited range of light emission and particularly poor photo-stability have limited the usage of organic dyes. After the introduction of semiconducting quantum dots many research groups have investigated inorganic quantum dots  for immunofluorscence labeling due to their high quantum yield and good photo physical and chemical stability. Despite the promising properties of semiconducting quantum dots for immunofluorescence labeling, cyto-toxicity is a critical problem in any live-cell or animal experiments. We have developed well-defined synthetic liposomes having immbeded organic nanoparticles with high quantum yield of 38% through directed self-assembly with functionalized diacetylene surfactants. The surface of the liposomes can be readily functionalized with various biological molecules. Selective targeting and dual color visualization of patterned avidin arrays were achieved by using the highly emissive functionalized nanoparticle liposomes to demonstrate the promising application of the organic nanoparticles for selective immunofluorescence labeling.

Two labs, “Polymerization, shaper memory polymers, and biopolymer gels” and “Nanomaterials”, have been developed and implemented to the core undergraduate laboratory courses. A new graduate course entitled "Functional Polymers: Design Principles and Applications" is currently under development for 2008 Winter term.