pdms chip interface product datasheet - · pdf filefabrication can be carried out using...

PDMS Chip Interface

product datasheet page

Description 2 Main Benefits 3 Specification 4 Cross-Sectional View 5 Geometry 6 Design Guidelines 8 Fabrication Guidelines 10 PDMS Chip Interface Starter Kit 11 Custom Options 11

The Dolomite Centre Ltd

PDMS_chip_interface_product_datasheet of 12

Part name Part number

PDMS Chip Interface 3200232

PDMS Chip Slide 50 x 25 mm (pack of 10) 3200264

PDMS Chip Slide 75 x 25 mm (pack of 10) 3200275

PDMS Chip Interface Starter Kit 3200273

Description

The PDMS Chip Interface (Part No. 3200232) allows users to create fast and reliable fluidic connections to PDMS-on-glass devices. The interface is designed to be used with a PDMS device (fabricated by the user) mounted to a PDMS Chip Slide with pre-drilled holes for fluidic connection (2 sizes available). Designing a PDMS device to fit the Dolomite format is quick and easy when following the design guidelines. Fabrication can be carried out using existing methods and equipment. The PDMS Chip Interface is compatible with the Multiflux® Linear Connector 4-way (Part No. 3000024) to provide 4 or 8 fluidic connections from 1/16” OD tubing to the PDMS chip.

PDMS Chip Interface (Part No. 3200232)

Full assembly of the PDMS Chip with two PDMS Chip Interfaces and two Linear Connectors 4-way (Part No. 3000024)

PDMS Chip Slide (Part No. 3200275)

Full assembly of the PDMS Chip with one PDMS Chip Interface and one Linear Connector 4-way (Part No. 3000024)



Main Benefits

• Robust and reliable connections • Provides a standard format for PDMS chip connections • Ideal for research use • Time savings in fabrication: no requirement to punch holes in PDMS • Time savings in use: no downtime due to leaks • Extends chip lifetimes • Widens applications for PDMS chips outside of research labs • Quick connect/disconnect • Low dead volume • Good microscope access • Accommodates wide range of PDMS device heights



Specification

Specification PDMS Chip Interface (Part No. 3200232)

Number of fluidic connections 4 or 8*

Compatible glass slide dimensions 50 x 25 x 1.3mm or 75 x 25 x 1.3mm (L x W x H)

Glass slide hole size Ø0.5mm

PDMS chip thickness options Up to 6mm

PDMS chip width options Up to 15mm

PDMS chip length options 10 – 44mm (50 x 25mm slide) 10 – 69mm (75 x 25mm slide)

Outside diameter of connection tubing 1.6mm (1/16inch)

Inside diameter of connection tubing 0.25, 0.5 or 0.8mm

Connection tubing material PTFE, FEP

Operating pressure 5bar**

Operating temperature range of seal -15 to 150°C***

Wetted materials Glass, PDMS, PTFE (or FEP), and perfluoroelastomer

Connector materials PEEK, Stainless Steel 316

*8 fluidic connections requires 2 x PDMS Chip Interface (Part No. 3200232) and 2 x Multiflux® Linear Connector 4-way (Part No. 3000024)

**Based on tests at 21C, PDMS chip may delaminate at lower pressure

***Assumes external surfaces of connector are at room temperature and tube I.D. <0.5mm.



Cross-sectional view

PDMS microfluidic device

Glass slide 75 x 25 mm (Part No. 3200275)

Linear Connector 4-way (Part No. 3000024)

PDMS Chip Interface (Part No. 3200232)

Detailed View of Sealing Interface

Microchannel in PDMS

Hole through glass slide

Perfluoroelastomer seal

PTFE or FEP tubing

Linear Connector 4-way (Part No. 3000024)

Glass slide 50 x 25 mm (Part No. 3200264)

PDMS microfluidic device PDMS Chip Interface (Part No. 3200232)

Left: Cross-sectional view with PDMS Chip Slide 50 x 25 mm (Part No. 3200264) Below: Cross-sectional view with PDMS Chip Slide 75 x 25 mm (Part No. 3200275)



Geometry

Geometry of PDMS Chip with one PDMS Chip Interface and one Linear Connector 4-way (Part No. 3000024)



Geometry of PDMS Chip with two PDMS Chip Interfaces and two Linear Connectors 4-way (Part No. 3000024)



Design Guidelines

In order to design a compatible PDMS device for use with the PDMS Chip Interface, it is necessary to follow 5 simple steps:

1. Decide if you require 4 fluidic connections or 8 fluidic connections.

a. 4 connections can be made with one PDMS Chip Interface and one Linear Connector 4-way. Two chips can be mounted to each slide.

b. 8 connections can be made with two PDMS Chip Interfaces and two Linear Connectors 4-way. One chip can be mounted to each slide.

2. Decide if your device length fits onto the standard 50 x 25 mm slide or if you require the longer 75 x 25 mm slide.

3. Design pad features in PDMS (recommended Ø1 mm) at a pitch of 3 mm to align with Ø0.5 holes in glass slide. These are the inlet and outlet ports.

4. Design outer dimensions of PDMS device ensuring that the boundaries are within the maximum width and length specified.

a. Maximum width is 15 mm. The maximum distance from outside port to PDMS side edges is 3 mm.

b. Maximum length depends on the number of fluidic connections (step 1) and the slide size selected (step 2). The maximum distance from inlet and outlet ports to PDMS back edge is 3 mm.

5. Design channel features in PDMS ensuring that all features to be viewed are positioned at least 3 mm from the inlet and outlet ports. Visible features should be outside of the red box in the diagram below.



Key design dimensions of PDMS replica if 4 fluidic connections are required and 2 chips of equal length are mounted onto the PDMS Chip Slide

Key design dimensions of PDMS replica if 8 fluidic connections are required (1 chip mounted onto PDMS Chip Slide)



Fabrication Guidelines

Fabrication of the PDMS device can be carried out using existing soft lithographic methods and equipment:

1. Fabricate a photoresist master (spin coat photoresist onto a silicon wafer, expose and develop).

2. Degas the PDMS mixture. Then cast the PDMS onto the master, cure and peel the wafer of PDMS replicas from the photoresist master. The PDMS height should be no greater than 6 mm.

3. Dice the wafer of PDMS replicas into individual chips ensuring that outer dimensions are within the allowed size.

4. Ensure that bonding surfaces of the glass and PDMS are clean then expose both surfaces to O2 plasma.

5. Align PDMS pad features to holes in the glass slide by eye or using low magnification eyepiece or viewer. Apply light pressure to create bond.

6. The completed PDMS chip can then be used with the PDMS Chip Interface.

Described above is a typical soft lithography process, but alternative methods may be used for the creation of channel features in the PDMS.

Example of PDMS replica mounted onto PDMS Chip Slide



PDMS Chip Interface Starter Kit

The PDMS Chip Interface Starter Kit (Part No. 3200273) contains the required components to start using the Multiflux® PDMS chip connection system.

The starter kit includes:

• 1 x PDMS Chip Interface – Part No. 3200232

• 1 x PDMS Chip Slide 50 x 25mm (pack of 10) – Part No. 3200264

• 1 x Multiflux® Linear Connector 4-way – Part No. 3000024

• 1 x Fluid Tube PTFE 1/16” OD, 0.5mm I.D, 10m Length – Part No. 3200067

Custom Options

Dolomite can offer custom connection systems for individual applications including thin layer glass slides for high magnification microscopy. Microscope holders are also available on request. The range of Dolomite services available covers all aspects of the development process from concept studies and feasibility testing to the design of commercial instruments. Please contact Dolomite to discuss your application.

PDMS Chip Interface Starter Kit (Part No. 3200273)

pdms chip interface product datasheet - · pdf filefabrication can be carried out using...

Documents