pengenalan kultur media bakteri
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PENGENALAN KULTUR MEDIA BAKTERI. DI EDIT OLEH PROF. DR.DRH .PRATIWI TS,MS. KULTUR MEDIA BACTERI UNTUK APA ?. APA YANG DIPERLUKAN?. KEBUTUHAN OKSIGEN. TUJUAN MELAKUKAN PENANAMAN. ISOLASI KULTUR MURNI. TIPE MEDIA TANAM. MEDIA CAIR. Kuliah berikut. fuchsine. Gram Staining Technique. - PowerPoint PPT PresentationTRANSCRIPT
PENGENALAN KULTUR MEDIA BAKTERI
DI EDIT OLEH PROF. DR.DRH .PRATIWI TS,MS
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KULTUR MEDIA BACTERI UNTUK APA ?
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APA YANG DIPERLUKAN?
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KEBUTUHAN OKSIGEN
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TUJUAN MELAKUKAN PENANAMAN
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ISOLASI KULTUR MURNI
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TIPE MEDIA TANAM
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MEDIA CAIR
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Kuliah berikut
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fuchsine
Gram Staining
Technique
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• Goal: To chemically (or physically) suppress unwanted microbes and encourage desired microbes.
Selective Media
Figure 6.9b, c
Mannitol salt agar : selective for halophiles with 7% salt (osmotic challenge) and differential for mannitol fermenters: good for skin bacterial cultures.
EMB Agar: kills gram positives with eosin and methylene blue, selective for gram negatives. Differential for lactose fermenters. Good for growing enterics.
McConkey Agar: supresses gram positives with crystal violet and bile salts; also differential for
MSA
EMB
MA
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• Distinguish between different species based on a metabolic ability.
Differential Media
Figure 6.9a
Blood agar(sheep’s blood) reveals if hemolytic
Mannitol salt agar contains the pH sensitive dye phenol red (yellow when acidic)
Se Sa
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Direct Measurements of Microbial Growth
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Estimating Bacterial Numbers by Indirect methods
• Direct Measures– Plate counts of viable bacterial forming colonies– Counting low viable bacterial numbers by filtration– Counting viable bacteria with Most Probable Number– Counting bacteria per ml in direct microscopy
• Indirect Measures– Turbidity/Absorbance with a spectrophotometer– Metabolic activity tracking conversion of colored
molecules/enyzme assay– Dry weight by weighing a set volume and knowing weight
of one cell
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Phases of Growth• Lag
– Adapt to nutrients• Log
– Active growth• Stationary
– Death = Growth rate• Death
– Nutrients consumed– pH too low (why?)
• Optimize curves in production
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Log Growth
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Figure 6.14
Growth Curve for Bacteria (Logarithmic Plot)
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Estimating Bacterial Numbers by Indirect methods
• Direct Measures– Plate counts of viable bacterial forming colonies– Counting low viable bacterial numbers by filtration– Counting viable bacteria with Most Probable Number– Counting bacteria per ml in direct microscopy
• Indirect Measures– Turbidity/Absorbance with a spectrophotometer– Metabolic activity tracking conversion of colored
molecules– Dry weight by weighing a set volume and knowing weight
of one cell
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