peptide hormones
DESCRIPTION
Peptide Hormones. Evolving Considerations for Biotechnology and Clinical Medicine Mainstream vs. Fad Chanda Zaveri, M.S. Founder & Chairman Activor Corporation. Peptide Hormones Features & Definition. Modify protein structure and state of activity - PowerPoint PPT PresentationTRANSCRIPT
Peptide Hormones
Evolving Considerations for Biotechnology and Clinical Medicine
Mainstream vs. FadChanda Zaveri, M.S.
Founder & ChairmanActivor Corporation
Peptide HormonesFeatures & Definition
Modify protein structure and state of activity
Are not metabolized by virtue of their activity
Hormone receptors – allosteric proteins
Peptide hormones – allosteric effectors
Peptide Hormone Physiology
• Act on cell membrane• Act via secondary mediators
• cAMP• Diacylglycerol • Calicum• Tyrosine kinase
Bioengineered Peptide Hormones
• Modification of existing protein• Consider primary, secondary or tertiary
structure as targets• Change in structure – new physiologic
effect
• Creation of novel protein
Secretagogues as Biotech Targets
• Control peptide hormone synthesis and secretion
• Three classes reported classically• Releasing factor hormones – GHRF• Central effectors
• Hypoglycemia, dopamine, deep sleep, amino acids
• Other peptide and steroid hormones• Cortisol, estrogen, thyroid hormone
HTA-5: Novel Peptide Hormone
• Primary structure derived from thymic hormone, with bioengineered modifications
• Physiological effect is dose dependent and cumulative• GHRF – low dose• Immunomodulation – high dose
TF-5 Literature Review
mitogenic T-cell response (Thurman, 1975)
• Modulates incidence of GVHD (Fast, 1990)
• Enhances NK activity of normal LGL (Serrate 1987)
and tumor bearing mice (Mastino, 1992)
• Increases tumor specific immunity cytotoxic T-lymphocyte response (Zatz & Goldstein,
1983)
antigen-presenting capacity of macrophages (Tzehoval, 1989)
• Stimulates proliferation of, and IL-6 production in, rat splenocytes (Attia & Badamchian 1993)
• Looking for “anti-aging” effect• Subjective Reports
• Improved memory • Enhanced sexual performance• Mood elevation• More restful sleep• Enhanced exercise performance• Decrease in incidence of hot flashes
hGH SupplementationWhy are people using this?
Theories of Aging
• Oxidative Stress Theory• Genetic Theory of Aging• Theory of Somatopause• Hormonal Theory of Aging
• Links aging to a decline in the body’s secretion of hormones WITHOUT any loss in its ability to respond to these hormones
hGH Supplementation (OFF LABEL)
• Objective Reports bone density immune function rate of wound healing HDL, LDL in LBM, LPL blood pressure cardiac output skin thickness and hair growth• General Insulin-like effect
HTA-5 Pilot Study Profile
• 15 Subjects• 7 Male & 8 Female
• Age Range: 32 - 70 years• Test Duration: 6 weeks• Preparation: HTA-5 + Lysine + Arginine• Dosing: 1x daily
• HTA-5: 20ng; Lys: 1200mg; Arg: 1200mg• Exclusion Criteria: [IGF-1]400ng/mL
Subjective Reports (combined study)
• Improved sleep patterns• Enhanced exercise stamina• Improvement in skin texture and
thickness• Decreased rate of hair loss
IGF-1 Physiology & Endpoint Considerations
• Glucose Metabolism • Exerts insulin-like effect
• Increases glycogen storage in SKM • Inhibits basal & insulin stimulated lipogenesis via LPL
• Cholesterol Metabolism• ? Increase in hepatic cholesterol receptors• ? Suppressed synthesis
• Osteoblast Metabolism• Binds to osteoblast receptor – stimulates new bone
formation
• IGF-2 > IGF-1
Male/Female IGF-1 Response
• HTA-5 stimulates IGF-1 response• Avg IGF-1: 40.4%; > 50 years: 56.8% • Co-administration with known RFs - Synergistic
IGF-1 response • DEDUCED: HTA-5 is GHRF
• Generalizations• Female IGF-1 response is double that of males• IGF-1 response is age dependent• Endpoint data suggests a heightened female
response
Total Cholesterol (mg/dL)
-16
-14
-12
-10
-8
-6
-4
-2
0
Aver
age
% D
ecre
ase
30-45 46-59 60+
Age Group Male Female
SubjectCholesterol Differenc
eInitial Final
1 231 181 -50
2 270 189 -81
3 257 241 -16
4 240 237 -3
5 241 231 -10
6 253 223 -30
7 219 209 -10
8 259 233 -26
9 239 217 -22
10 241 225 -16
11 214 203 -11
12 237 215 -22
13 218 211 -7
14 247 222 -25
15 251 239 -12
Male/Female Cholesterol Response
• Avg Total Cholesterol: 23 mg/dL• Decrease in serum cholesterol
• No dietary modifications• No change in medical regimen, if any• No lifestyle modifications
Bone Density (g/cm2)
• Radial ultrasound
• Average Increase• 6.8% HTA-5• 12.6% HTA-5 + AA
MALE
FEMALE
Body Composition Response
• Objective changes in TBC •Avg % ATM: 14.8%•Avg % LBM: 4.1%
•Slight across age groups•Avg % body weight: 13.2%
•No dietary or lifestyle modifications