peripheral blood smear examinationfamilymed.sbmu.ac.ir/uploads/hematology dr momtazmanesh.pdf ·...
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Peripheral Blood Smear
Examination
Momtazmanesh MD.
Ped. Hematologist & Oncologist
Loghman General Hospital
1395
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Peripheral
Blood Smear
• A peripheral blood smear is a snapshot of the cells that
are present in the blood at the time that the sample is
obtained.
Momtazmanesh M.D.
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When to Get Tested?
• When CBC results are abnormal. (flags)
• When a deficiency, disease, or disorder that can affect
blood cell production is suspected.
• As a quality control tool for the results of CBCs.
Momtazmanesh M.D.
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Blood Smear prepration and staining:
1-Within 3 hours
2-Tradition wedge smear
3-Romanowsky stain (usually
Wright's, Giemsa)
Momtazmanesh M.D.
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Momtazm
anesh
M.D.
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Making and Staining a Blood Smear
• Place a drop of blood approximately 4 mm in diameter on
the slide (near the end)
• Spread the drop by using another slide (called here the
“spreader”)
• placing the spreader at a 45° angle and BACKING into
the drop of blood.
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PBS
• To make a short smear, hold the spreader at a steeper
angle, and to make a longer smear, hold it closer to the
drop.
• Prevent blood from reaching the extreme edges of the
slide.
• A smear with wavy lines or blanks spots should be
discarded, and a new smear made.
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Evaluation of Blood Cells
•Selection of a suitable area
•Traditional sequence:
White cells
Red cells
Platelets
Parasites
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Momtazmanesh M.D.
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Evaluation of White Blood Cells
Total counts
Diff. counts
I:T ratio
Abnormal WBCs
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Pelger Huet
Momtazmanesh M.D.
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Momtazmanesh M.D.
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Red Blood Cells
• Size
• Color
• Shape
• Arrangement
• Inclusions
• Young RBCs
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Hypochromia & microcytosis
• Iron deficiency anemia
• Thalassemia, α or β
• Sideroblastic anemia
• Chronic disease
• Infection
• Inflammation
• Cancer
• Lead toxicity
• Hemoglobin E trait
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Macrocytosis
• Normal newborn
• Megaloblastic anemias
• Post-splenectomy
• Liver disease
• Hereditary aplastic anemia
• Hypothyroidism
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Momtazmanesh M.D.
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Target cells
• Thalassemic syndromes
• Hb C, SC, S-Thal, E, D
• Obstructive liver disease
• Postsplenectomy or hyposplenic states
• Severe iron deficiency
• LCAT (lecithin/cholesterol acyltransferase)
deficiency: (corneal opacifications, proteinuria,
target cells, moderately severe anemia)
• Abetalipoproteinemia
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Momtazmanesh M.D.
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Spherocytes
• Hereditary spherocytosis
• ABO incompatibility
• Autoimmune hemolytic anemia
• Burns
• Posttransfusion
• G-6-PD Deficiency
• Microangiopathic hemolytic anemia (MAHA)
• SS disease
• Hypersplenism
• Pyruvate kinase deficiency
• Water-dilution hemolysis
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Acanthocytes (spur cells)
• Liver disease
• DIC and other MAHA
• Postsplenectomy or hyposplenic state
• Vitamin E deficiency
• Hypothyroidism
• Abetalipoproteinemia: (fat malabsorption, retinitis
pigmentosa, neurologic abnormalities)
• Malabsorptive states
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Momtazmanesh M.D.
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Echinocytes (burr cells)
• Artifact
• Uremia
• Dehydration
• Liver disease
• Pyruvate kinase deficiency
• Peptic ulcer disease or gastric carcinoma
• Immediately after red cell transfusion
• Rare congenital anemias due to decreased
intracellular potassium
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Momtazmanesh M.D.
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Schistocytes • Helmet, triangular shapes, or small fragments
• DIC & MAHA (HUS, TTP)
• Severe hemolytic anemia (e.g., G6PD deficiency)
• Prosthetic cardiac valve, abnormal cardiac valve, cardiac
patch, coarctation of the aorta
• Connective tissue disorder (e.g., SLE)
• Kasabach–Merritt syndrome
• Purpura fulminans
• Renal vein thrombosis
• Homograft rejection
• Uremia, acute tubular necrosis, glomerulonephritis
• Malignant hypertension
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Momtazmanesh M.D.
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Elliptocytes
• Hereditary elliptocytosis
• Iron deficiency (increased with severity, hypochromic)
• SS disease & SA trait
• Thalassemia major
• Severe bacterial infection
• Leukoerythroblastic reaction
• Megaloblastic anemias
• Any anemia may occasionally present with up to 10%
elliptocytes
• Malaria
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Momtazmanesh M.D.
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Teardrop cells
• Newborn
• Thalassemia major
• Leukoerythroblastic reaction
• Myeloproliferative syndromes
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Momtazmanesh M.D.
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Stomatocytes
• Normal (in small numbers)
• Hereditary stomatocytosis
• Artifact
• Thalassemia
• Acute alcoholism
• Rh null disease (absence of Rh complex)
• Liver disease
• Malignancies
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Blister cells
• G6PD deficiency (during hemolytic episode)
• SS disease
• Pulmonary emboli
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Basophilic stippling
• Hemolytic anemias (e.g., thalassemia trait)
• Iron-deficiency anemia
• Lead poisoning
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Cabot’s Ring bodies
• Pernicious anemia
• Lead toxicity
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Momtazmanesh M.D.
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Howell–Jolly bodies
• Postsplenectomy or hyposplenia
• Newborn
• Megaloblastic anemias
• Dyserythropoietic anemias
• A variety of types of anemias (rarely iron-deficiency
anemia, hereditary spherocytosis)
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Heinz bodies
• G6PD Deficiency
• Normal in newborn
• Thalassemia (alpha)
• Asplenia
• Chronic liver disease
• Heinz body hemolytic anemia
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Nucleated red blood cells
• Not normal in the peripheral blood beyond the first week
of life
• Newborn (first 3–4 days)
• Intense bone marrow stimulation
• Hypoxia (especially postcardiac arrest)
• Acute bleeding
• Severe hemolytic anemia (e.g., thalassemia, SS
hemoglobinopathy)
• Congenital infections (e.g., sepsis, congenital syphilis,
CMV, rubella)
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Leukoerythroblastic reaction
• extramedullary hematopoiesis and bone marrow
replacement; most commonly:
• Leukemia
• Solid tumor
• Fungal and mycobacterial infection
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Momtazmanesh M.D.
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Platelet
Normal & Abnormal
Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Bernard-Soulier Syn.
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Grey Platelet Syndrome
Momtazmanesh M.D.
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Momtazmanesh M.D.
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Bone Marrow Aspiration
Momtazmanesh M.D.
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Normal Bone Marrow
Momtazmanesh M.D.
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BMA in ITP
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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Momtazmanesh M.D.
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