pertemuan 9-biological processing...

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Biological processing Anuraga Jayanegara Department of Nutrition and Feed Technology Outline A. Silage B. Fungal treatment C. Yeast treatment D. Bacterial treatment E. Enzyme treatment F. Probiotics and prebiotics Department of Nutrition and Feed Technology A. Silage Silage is forage conserved by anaerobic fermentation What is occurring during ensiling: - Plant material is chopped and packed into a silo to exclude oxygen - The respiring plant cells use up the existing oxygen and then die - Proliferation of lactic acid-producing anaerobic bacteria - Fermentation continues until the accumulation of lactic acid depresses the pH to approximately 4 - Further bacterial growth is inhibited, the silage is stabilized Department of Nutrition and Feed Technology How to produce a good quality silage? - Sufficient population of lactic acid bacteria - Contain sufficient fermentable carbohydrate --> it might be necessary to add grain or molasses as a source of substrate for fermentation - Legumes have a higher buffering capacity than grasses --> require a higher level of soluble carbohydrate - Optimum water soluble carbohydrate (WSC) content: 3 – 5% DM - Optimum moisture content --> dry matter between 25 to 35% - Low moisture material --> exclusion of oxygen is difficult; molding and heating may occur - High moisture material --> putrefaction --> Clostridia fermentation producing large amounts of butyric acid and offensive-smelling amines such as tryptamine and histamine

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Page 1: Pertemuan 9-Biological processing silageanuragaja.staff.ipb.ac.id/files/2012/02/Pertemuan-9-Biological... · Biological processing Anuraga Jayanegara Outline A. Silage B. Fungal treatment

Bio

log

ica

l p

roce

ssin

g

An

ura

ga

Ja

ya

ne

ga

ra

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Ou

tlin

e

A.

Sil

ag

e

B.

Fu

ng

al

tre

atm

en

t

C.

Ye

ast

tre

atm

en

t

D.

Ba

cte

ria

l tr

ea

tme

nt

E.

En

zym

e t

rea

tme

nt

F.

Pro

bio

tics

an

d p

reb

ioti

cs

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

A.

Sil

ag

e

Sil

ag

e i

s fo

rag

e c

on

serv

ed

by

an

ae

rob

ic f

erm

en

tati

on

Wh

at

is o

ccu

rrin

g d

uri

ng

en

sili

ng

:

-P

lan

t m

ate

ria

l is

ch

op

pe

d a

nd

pa

cke

d i

nto

a s

ilo

to

exc

lud

e o

xyg

en

-T

he

re

spir

ing

pla

nt

cell

s u

se u

p t

he

exi

stin

g o

xyg

en

an

d t

he

n d

ie

-P

roli

fera

tio

n o

f la

ctic

aci

d-p

rod

uci

ng

an

ae

rob

ic b

act

eri

a

-F

erm

en

tati

on

co

nti

nu

es

un

til

the

acc

um

ula

tio

n o

f la

ctic

aci

d d

ep

ress

es

the

pH

to

ap

pro

xim

ate

ly 4

-F

urt

he

r b

act

eri

al

gro

wth

is

inh

ibit

ed

, th

e s

ila

ge

is

sta

bil

ize

d

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Ho

w t

o p

rod

uce

a g

oo

d q

ua

lity

sil

ag

e?

-S

uff

icie

nt

po

pu

lati

on

of

lact

ic a

cid

ba

cte

ria

-C

on

tain

su

ffic

ien

t fe

rme

nta

ble

ca

rbo

hy

dra

te -

-> i

t m

igh

t b

e n

ece

ssa

ry t

o

ad

d g

rain

or

mo

lass

es

as

a s

ou

rce

of

sub

stra

te f

or

ferm

en

tati

on

-Le

gu

me

s h

av

e a

hig

he

r b

uff

eri

ng

ca

pa

city

th

an

gra

sse

s --

> r

eq

uir

e a

hig

he

r le

ve

l o

f so

lub

le c

arb

oh

yd

rate

-O

pti

mu

m w

ate

r so

lub

le c

arb

oh

yd

rate

(W

SC

) co

nte

nt:

3 –

5%

DM

-O

pti

mu

m m

ois

ture

co

nte

nt

-->

dry

ma

tte

r b

etw

ee

n 2

5 t

o 3

5%

-Lo

w m

ois

ture

ma

teri

al

-->

exc

lusi

on

of

oxy

ge

n i

s d

iffi

cult

; m

old

ing

an

d

he

ati

ng

ma

y o

ccu

r

-H

igh

mo

istu

re m

ate

ria

l --

> p

utr

efa

ctio

n -

-> Clostridia

ferm

en

tati

on

pro

du

cin

g l

arg

e a

mo

un

ts o

f b

uty

ric

aci

d a

nd

off

en

siv

e-s

me

llin

g a

min

es

such

as

try

pta

min

e a

nd

his

tam

ine

Page 2: Pertemuan 9-Biological processing silageanuragaja.staff.ipb.ac.id/files/2012/02/Pertemuan-9-Biological... · Biological processing Anuraga Jayanegara Outline A. Silage B. Fungal treatment

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Ma

in c

rop

s u

se f

or

sila

ge

:

-G

rass

es

-Le

gu

me

s

-F

ield

co

rn

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Sil

ag

e a

dd

itiv

es:

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Lact

ic a

cid

ba

cte

ria

-A

gro

up

of

ba

cte

ria

th

at

pro

du

ce l

act

ic a

cid

-G

row

at

pH

aro

un

d 4

.0 –

6.8

-->

ce

rta

in s

pe

cie

s ca

n b

e

do

wn

to

pH

3.5

-T

em

pe

ratu

re b

etw

ee

n 5

–5

0oC

-->

op

tim

um

of

ma

ny

spe

cie

s is

aro

un

d 3

0oC

Page 3: Pertemuan 9-Biological processing silageanuragaja.staff.ipb.ac.id/files/2012/02/Pertemuan-9-Biological... · Biological processing Anuraga Jayanegara Outline A. Silage B. Fungal treatment

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Lact

ate

sil

ag

e:

-La

ctic

aci

d b

act

eri

a d

om

ina

te t

he

fe

rme

nta

tio

n p

roce

ss

-Lo

w p

H -

-> t

yp

ica

lly

be

twe

en

3.7

–4

.2

-H

igh

co

nce

ntr

ati

on

of

lact

ic a

cid

/la

cta

te (

8 –

12

%)

-Lo

w f

orm

ate

, a

ceta

te,

pro

pio

na

te a

nd

bu

tyra

te

-M

ay

co

nta

in m

an

nit

ol

an

d e

tha

no

l --

> h

ete

rofe

rme

nta

tiv

e

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Page 4: Pertemuan 9-Biological processing silageanuragaja.staff.ipb.ac.id/files/2012/02/Pertemuan-9-Biological... · Biological processing Anuraga Jayanegara Outline A. Silage B. Fungal treatment

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Ace

tate

sil

ag

e:

-U

nd

er

cert

ain

co

nd

itio

n,

ace

tate

ma

y d

om

ina

te t

he

fe

rme

nta

tio

n

-T

he

pro

ba

bil

ity

is

hig

he

r in

th

e s

ila

ge

ma

de

fro

m t

rop

ica

l g

rass

es

vs

tem

pe

rate

gra

sse

s

-Lo

w l

act

ate

-H

igh

ace

tate

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Bu

tyra

te s

ila

ge

:

-p

H i

s n

ot

low

en

ou

gh

-->

sa

cch

aro

lyti

c Clostridia

de

ve

lop

me

nt

-->

ferm

en

tin

g l

act

ate

an

d t

he

re

ma

inin

g W

SC

in

to b

uty

rate

-->

ev

en

hig

he

r

pH

-->

pro

teo

lyti

c Clostridia

de

ve

lop

me

nt

-->

pro

du

cin

g a

mm

on

ia

-Clostridia

gro

ws

at

hig

h m

ois

ture

co

nte

nt

(DM

aro

un

d 1

5%

)

-Lo

w W

SC

an

d h

igh

bu

ffe

rin

g c

ap

aci

ty s

up

po

rt t

he

gro

wth

of Clostridia

-p

H o

f b

uty

rate

sil

ag

e:

5 –

6

-Lo

w l

act

ate

-Lo

ss o

f n

utr

ien

ts d

ue

to

co

nv

ers

ion

in

to C

O2,

H2

an

d N

H3

-U

np

ala

tab

le t

o t

he

an

ima

ls -

-> n

eg

ati

ve

co

rre

lati

on

be

twe

en

DM

in

tak

e

an

d N

-am

mo

nia

pro

du

ced

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Page 5: Pertemuan 9-Biological processing silageanuragaja.staff.ipb.ac.id/files/2012/02/Pertemuan-9-Biological... · Biological processing Anuraga Jayanegara Outline A. Silage B. Fungal treatment

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy

Than

k yo

u fo

r yo

ur attention!

Than

k yo

u fo

r yo

ur attention!

“Impian kita kemarin adalah

kenyataan hari ini, dan impian kita

hari ini adalah kenyataan hari esok“

De

pa

rtm

en

t o

f N

utr

itio

n a

nd

Fe

ed

Te

chn

olo

gy