pharmacological evaluation of clerodendrum philippinum schauer

7/30/2019 Pharmacological Evaluation of Clerodendrum Philippinum Schauer

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(08.00-16.00 h). The institutional animal ethicalcommittee approved the protocol of the study. Theanimals were divided into five groups each contain-

ing six mice. The groups of mice were assigned toreceive one of the following, Group I (vehicle (0.5 %tween 80) 1 ml/100g of body weight, p.o); Group II

(EECP, 125 mg/kg, p.o) ; Group III (EECP, 250 mg/kg, p.o) and Group IV (Diazepam, 0.5 mg/kg, p.o).

Drug and chemicals

Diazepam (Ranbaxy Laboratories Ltd, Mumbai)was used as the standard anxiolytic agent. Petroleum

ether 60-800C and ethanol were procured from Merck

Ltd (India).

Acute oral toxicity studies

The OECD guidelines 425 were followed for theacute oral toxicity study for fixing the dose. The doselevel up to 2500mg/kg of C.philippinum were notproduced any mortality on oral administration. So the125 and 250mg/kg doses were fixed for the study [5].

Behavioral parameters used to test anti-anxiety

activity

In Elevated plus-maze test an entry was definedas having all four paws within the arm. EPM consistof two open arms (16x5 cm) crossed with two closed

arms (16x5x12cm) [6]. The arms were connected

together with a central square of 5x5 cm. The appara-

tus was elevated to the height of 25cm in a dimlyilluminated room. Mice were treated with EECP(125, 250mg/kg), diazepam (1mg/kg, p.o) and vehicle30 min before being placed individually in the centerof the EPM facing a closed arm. The time spent inboth the open and closed arms were recorded for 5min. The number of entries in to the open and closedarms was also counted during the test.

Locomotor activityThe Locomotor activity was studied using acto-

photometer [6]. The movement of the animal inter-rupts the beam of light falling on a photocell at which

the count was recorded and displayed digitally. Eachmouse was placed individually in the actophotometerfor 10 min and the basal activity was obtained. Sub-sequently the animals were divided in to 4 groupseach consists of six animals. Vehicle, EECP (125mg/kg, 250 mg/kg), (or) diazepam (1mg/kg, p.o) wasadministered and after 30 min of administration, the

mice were placed again in the actophotometer forrecording the activity score.

Statistical analysis:

Results are expressed as mean + S.E.M. The sta-

tistical analysis of data was done using the one way

analysis of variance (ANOVA) followed by Dunnetts

test .A probability level less than 0.05 was consideredstatistically significant.

RESULTSPhytochemical screening of EECP revealed the

presence of phenolic compounds, phenyl propanoid,

flavanoids and sterols. Oral administration of EECPup to 2.5 g/kg did not produce any toxic effects inmice. No mortality was observed and EECP wasfound to be safe at the given doses. Diazepam treatedrats showed a significant increase (P > 0.01) in thenumber of open arm entries, percentage ratio of open

arm to total arm entries, time spent in the open armsand number of rears in the open arms. EECP treated

rats exhibited a significant increase (P > 0.01) in thenumber of open arm entries, time spent in the open

arm, percentage ratio of open arm to total arm entriesand number of rears in the open arms at both doselevels (125 & 250mg/kg) when compared with nor-mal animals (Table 1). EECP in doses of 125 and

250mg/kg produced significant (P


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Lalitha et al / International Journal of Pharmaceutical Research 2010 2(2) 13-15

mechanism of anxiolytic action of EECP could bedue to the binding of any of these phytochemicals tothe GABA- BZD complex [9].

Table 1: Effect ofClerodendurm philippinum on behavior of rats in elevated plus maze paradigm

Values are mean SEM, n=6,a

-P

pharmacological evaluation of clerodendrum philippinum schauer

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