phylogenetic reconstruction of the paper wasp genus polistes (hymenoptera: vespidae)
TRANSCRIPT
Phylogenetic Reconstruction of the Paper Wasp Genus Polistes (Hymenoptera: Vespidae)
Department of Ecology, Evolution, and Organismal Biology (EEOB)
Eusociality
Cooperative brood care Overlapping generations
Division of labor
1.Abundance and environmental range
2.Exploitation of human populated areas
3.Relatively small colonies
4.Primitively Eusocial
Cooperative breeding
Sheenans et al . 2014
Variation in number of foundresses
Evolution of Cooperative Breeding
Sheehan et al. 2015
Santos et al 2015
Maximum Parsimony 66 species: Outgroup: 8 speciesPolistes: 58 species
3 Nuclear genes: 28S rRNA, Histone 3 (H3) and Elongation factor I alpha (EFI – α)
3 mitochondrial genes: COI, 12S rRNA and 16S rRNA
Morphological data:
Current Polistes Phylogeny
Estimation of Polistes Emergence 1. Produce a more resolved Polistes phylogeny
2. Bayesian Statistical framework
3. Time-calibrated phylogeny (inclusion of fossil)
Methods and Data
Cockerell 1914
Sequence data and Fossil Data
Pickett and Wenzel 2004Santos et al 2015
Species (SF) Citation Age Location
P. industrius Theobald 1937 33.7 – 28.5 mya
Cereste France
P. kirbyanus Cockerell 1914 11.2 – 7.1 mya
Oeningen Germany
P. primitive Heer 1865 11.2 – 7.1 mya
Oeningen Germany
P. vergnei Piton 1940 65 – 54.8 mya Menat France
P. signata Statz 1936 28.5 – 23.8 mya Rott-am-SiebengebirgeGermany
Protopolistes oblitus Cockerell 1921 37 – 33.7 mya Isle of Wight, UK
Agelaia electra Carpenter & Grimaldi, 1997
20.5 – 16.4 mya Dominica, Dominican Republic
Fossil Data
Analysis• BEAST2 • Fossilized Birth-Death Process
• Allows inclusion of fossil• Uniform Partition
• Model testing• Constraint on genus Polistes
• Removal of species• Thinning of Beast2 output
Heath et al. 2014
Bayesian Statistical framework
Computational Resources4 Independent runs
MCMC runs- 1,000,000,000Burn-in: 20%
ISU High Performance Computing (HPC)Speedy : 24 core, 256 GB RAM 3.40 GHz Intel
Xeon CPU
Tree Node Support
Polistes Node
High support
Low-support
Produce a more resolved Polistes phylogeny
Time–scale (millions of years ago) PresentPast
Emergence of Polistes (64.5 mya)
Time-calibrated phylogeny (inclusion of fossil)
Conclusion1. High support nodes for Polistes (Higher resolution)
2. First Time-calibrated phylogeny
3. Better understanding of evolution of Polistes
4. New tool for biologists
Provide new understanding of evolution of eusociality
Future Directions: Biogeography
New World Polistes
Santos et al. 2015
Future Directions: Niche modeling
Natural Range Potential Range
P. dominula
Questions?
Acknowledgments FundingGMAP FellowshipNSF Grants:DEB-1556853 & DEB-1556615
Computing resources ISU High Performance Computing (HPC)
ISU Interdepartmental Genetics Program