7/28/2019 Phytochemical Prospection and Biological Activity Evaluation of Ethanolic and Hydroalcoholic Extracts From the Lea

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PHYTOCHEMICAL PROSPECTION AND BIOLOGICAL ACTIVITY

EVALUATION OF ETHANOLIC AND HYDROALCOHOLIC EXTRACTS

FROM THE LEAVES OF Hibiscus ros a-sinensisL.

Cayo Jorge Amaral Farias1, Ana Carolina Moraes de Santana

2, Rute Mendona

Xavier de Moura3

1Student of Pharmacy Course at Faculdades do Sul-FACSUL/UNIME-Itabuna-Bahia

2Professor of Pharmacy Course at Faculdades do Sul-FACSUL/UNIME-Itabuna-Bahia

3Coordinator of Pharmacy Course at Faculdades do Sul FACSUL/UNIME-Itabuna-Bahia

The species Hibiscus rosa sinensis L., belongs to the Malvaceae family, it hasbeen used in the ornamentation and as an alternative source in the search of

natural dyes. The hibiscus has studies that observed the hypoglycemic,hypocholesterolaemic and anticonvulsant actions. The aim of this work was tomake the phytochemical prospection and the biological activity test for thespecies H. rosa-sinensis L. The species was collected at 4CEPLAC area inIlhus, State of Bahia. The analysis was performed in the pharmacognosy andclinical research laboratories of FACSUL-UNIME Itabuna. Were prepared twoextracts: The ethanolic (A) and the hydroalcoholic (B) in concentrations of 1.25,2.5 and 5% by macerating of leaves. The phytochemical prospection wasconducted according to the Brazilian Pharmacopoeia. The antimicrobial activitytest was performed in vitro per diffusion in disks against strains of Escherichiacoli(ATCC 29522), Pseudomonas aeruginosa (ATCC 27853), Staphylococcus

aureus (ATCC 29523) and ceftriaxone as a positive control. The Bacterialcultures were grown on Mueller Hinton agar at 37 C for 24 hours. The resultsof prospection were positive for tannins, cardioactives and anthraquinones inboth extracts and positive for flavonoids just in the extract (B). The extracts Aand B of 1.25% didnt present inhibitory activity, but the extracts of 2.5% and5% presented inhibitory activity except against P. aeruginosa (ATCC 27853).The inhibition zones obtained were: Extract A and B of 1,25%: forE. coli7mm,forP. aeruginosa 7mm and forS. aureus 8mm; extract A of 2,5%: forE. coli7mm, forP. aeruginosa 6mm and forS. aureus 9mm and extract B of 2,5%: forE. coli11mm, forP. aeruginosa 6mm and forS. aureus 8mm; extract A of 5%:forE. coli10mm, forP. aeruginosa 6mm and forS. aureus 10mm and extract Bof 5% forE. coli14mm, forP. aeruginosa 6mm and forS. aureus 9mm. In thiswork was found the presence of special metabolites classes withpharmacological activity and the presence of inhibition zones in the diffusiontest with disk that represents an indicative of antimicrobial activity, other testsare needed for confirmation.

Keywords: Hibiscus, Phytochemical prospection, Biological activity

Financial Support: FACSUL UNIME/Itabuna

[4] - Executive Planning Commission of Cocoa Farming