PINK PEPPERSLIMSpicy Fats Burner

CCOSMOS

Pink Pepperslim is a slimming oil derived from Pink Pepper with powerfullipolytic properties.

A concentrated oil from pink berries, Pink PepperSlim sets itself apart from traditional fat burners due toits pre-lipolytic action. Its pre-lipolytic activity focuses on large lipid droplets. This slimming agent reduces their protection tomake them more available to lipases. It also stops them forming instead of small lipid droplets which areeasier to burn. Prepared in this way, its lipolytic action is multiplied and it generates significant centimetreloss in both men and women.Finally, the slimming effect of Pink PepperSlim is rounded off by stimulating micro-circulation.

Pink PepperSlim is a pure oil extracted from Schinus terebinthifolius seeds using the supercritical CO2 technique.

The false pepper plant Schinus terebinthifolius is a specie of tree whose berries provide a spice known as “pepperrosé”, “pink berries” or “Aroeira. It originated in Brazil and is nowadays cultivated in the island Réunion (next toMadagascar) for its berries known as the “Pink Gold of Réunion”.

The tree can measure up to 15 metres high. Its leaves are evergreen, aromatic, creased and can reach 15 cm inlength. They give off a peppery odour or an odour of terebinth. The off-white flowers hang in bunches. From these,a discretely perfumed honey is produced. The pink berries, aromatic fruits, coloured coral red in summer, are 8 mm in diameter and have a peppery smell. The tree secretes an aromatic resin which is also called “incense”.

Pink pepper has a fairly sweet taste, almost sugary at first, then aromatic. They are often sold preserved in brineor used as dried berries. They are eaten whole or crumbled, i.e. carefully crushed between the fingers.

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PINK PEPPERSLIMSpicy Fats Burner CCOSMOS

The different stages of fat storage

1 - Pre-adipocyte differentiation

Differentiation of pre-adipocytes, which up till that point arenot capable of storing fat, in the young adipocyte is the firststep in the fat storage process.

Fats are taken on by a protein called adipophilin and stored assmall lipid droplets.

2 - Maturing of the young adipocyte

Development of young adipocytes into mature adipocytes isthe second fat storage step.

The second step is accompanied by a fall in the level ofadipophilin and an increase in the level of Perilipin A.

Perilipin A takes on the fat molecules during the second step.Fats are stored in the form of a single large lipid droplet.

In addition to take on fats, Perilipin A protects large lipiddroplets. By distributing itself over the droplet surface, itcreates a sort of cellular shield which restricts access bylipases to the fat reserves.

Pink PepperSlim : two fold lipolyticaction

1 - Pre-lipolytic action

To burn fat, it is important that the lipases can access themeasily. Also, it is easier for them to burn up small lipid dropletsthan large ones.

Inhibiting Perilipin A synthesis acts on both these factors:- it reduces the protection on large lipid droplets making themeasier to metabolise- it reduces regulation of fat molecules to form large lipiddroplets and so promotes formation of small lipid droplets whichare much easier to metabolise.

2 - Lipolytic action

Since the fat reserves are now more accessible, lipolysis is moreeffective:

- increased amount of glycerol released- increased fatty acid quantities released

OBSERVE

MEMO OF CUTANEOUS BIOLOGY

Adipophilin Perilipin A

Process of Differentiation

Leve

l of

exp

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sio

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Adipophilin Perilipin A

Process of Differentiation

Leve

l of

exp

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sio

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1 2

Pre adipocyteFat storage: 0

Young adipocyteFat storage: +

Mature adipocyteFat storage: +++

1 2

Pre adipocyteFat storage: 0

Young adipocyteFat storage: +

Mature adipocyteFat storage: +++

PRE-LIPOLYTIC ACTION

Pink PepperSlim inhibits Perilipin Aexpression by 23%

This inhibition leads to:

• reduced protection of large lipid droplets against lipolytic agents• reduced ability to take on fats during development of small lipiddroplets into large lipid droplets

Pink PepperSlim increases adipophilin levelby 60%

This increase directly results from Perilipin A inhibition. Sincesmall lipid droplet maturation is restricted, the number of smalldrops increases compared to large drops and adipophilin levelincrease accordingly.

Pink PepperSlim stops the formation of large lipid droplets in favour of small ones

The colouring of lipid droplets by the red oil shows the inhibitoryeffect of Pink PepperSlim on large lipid droplets formation and fatstorage.

Differentiated human pre-adipocyte culturesAnalysis of Perilipin A and Adipophilin expression: RT-qPCRPerilipin A and Adipophilin visusalisation: ImmunofluorescenceFat storage visualisation: red oil

IN-VITRO TESTS

LIPOLYTIC ACTION

Pink PepperSlim displays powerful lipolyticactivity.

From 0.0025%:Release of glycerol: +24%Release of fatty acids: +1253%

At 0.005% :Release of glycerol: +91%*Release of fatty acids: +1737%*

*p<0.05 Student TestDifferentiated human pre-adipocyte culturesFatty acid and glycerol amounts released measured by optical density

Pink PepperSlim displays greater lipolytic effectiveness than reference molecules

Theophylline: 0.018%Isoproterenol: 0.00002%Caffeine: 0.019%Pink Pepperslim: 0.005%

With its pre-lipolytic action, Pink PepperSlim sets upoptimum lipolytic conditions: small lipid droplets are in themajority which are easier to metabolise than large onesand the protection on already formed large lipid droplets isweakened.Its lipolytic action is facilitated and more powerful than thatof reference lipolytic molecules.

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Pink PepperSlim0,0025%

Pink PepperSlim

0,005%

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Release of Glycerol

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+81% +158% +200%

+1737%

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PepperSlim

Control Pink PepperSlim0,005%

20 μm 20 μm

20 μm 20 μm

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Land plant origin Liposoluble

active ingredientNatural Ecocert

validation

in-vitro test in-vivo test

Due to its pre-lipolytic action, Pink PepperSlim optimises its effects andmetabolises up to 1000% more fatty acids. This powerful lipolytic activitygenerates size reductions in men and women of up to 2 cm in 28 days.

SLIMMING ACTION

Protocol:

- Application of a cream containing 0.1% Pink PepperSlim twice a day for 28 days- 18 female volunteers: reductions measured in centimetres on the waist, hips and thighs- 7 male volunteers: reductions measured in centimetres on the abdominal belt line

REVEAL

CLINICAL TEST

RESULT:

The use of Pink PepperSlim led to a loss in centimetre in men and women of up to 2 cm.

*p<0.05 Student Test

EFFECT ON MICROCIRCULATION

Protocol:

- Single application of a cream containing 0.1% Pink PepperSlim on the forearm- 10 volunteers: variation in speed of blood circulation measured by the Doppler Effect and by resistance toblood circulation

RESULT:

Pink PepperSlim activates microcirculation by 16% after 1 hour without any vasodilation

Resistance to blood flow is used to determine whetherthe product has effects on blood vessel diameter.Pink PepperSlim induces a slight increase in resistanceafter 30min, which then normalises after 60min.This weak variation demonstrates this absence of bothvasoconstrictive and vasodilation effect

Size reduction in women Size reduction in men

-2,5 -2,0 -1,5 -1,0 -0,5 0,0Centimetre reduction (in cms)

Average loss

Thighs- 0,15 cm

Hips

Waist

Max. loss- 0,7 cm

- 0,7 cm

- 2 cm

- 0,4 cm

- 2,2 cm

-0,8 -0,6 -0,4 -0,2 0,0

Centimetre reduction (in cms)

Average loss

Belt line

Max. loss

- 0,4 cm

- 0,7 cm

T60 min

+10,8%

+13,9%

+15,6%*+16,5%*

CCOSMOS COSMOS

Validation

RP

DO

C12

1GB

CODIF Recherche & Nature is committed to sustainable development.

All our wastewater is processed and purified using filtrating gardens.A succession of airtight horizontal and vertical filters is used to depollute

wastewater by the roots of plant species chosen for their draining properties.

Iris, water mint, flowering rush, willows etc… now form part of the image of the brand which is recognized for its commitment to the preservation of natural resources

PINK PEPPERSLIM: Spicy Fats Burner

Cosmetic Actions

= Inhibits Perilipin A synthesis= Stops formation of large lipid droplets= Activates release of glycerol and fatty acids= Reduces the circumference of the waist, hips and thighs in women= Reduces the abdominal belt line in men= Stimulates microcirculation

INCI Name

Schinus terebinthifolius seed extract

Recommended % of use

PINK PEPPERSLIM : 0.1%

Technical department : infotech@codif-recherche-et-nature.comCommercial department : commercial@codif-recherche-et-nature.com

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CCOSMOS