PowerPoint Presentation

Flexible and Comprehensive Analytical Platform for PrecisionClinical Research, Pharma R&D and Education

Opportunity: Omics Data Analysis Market SegmentationSource: Bioinformatics Market by Sector Global Forecast to 20201Market

Global bioinformatics market size, $B (forecast)CAGR = 20.9%

GenomicsProteomicsTranscriptomicsMetabolomicsMicrobiomeOtherRelative shares and growth of market segments, %CAGRRank

2Challenge

3

From Big Biomedical Data to Actionable Results SolutionMachine Learning

Feature extractionIntegrationPredictive modelingData miningSimulation

Biological Meaning

Pathway AnalysisVisualizationRelation to PhenotypeHypothesis Building

Decision making

Clinical trialsTarget CandidateMolecular DiagnosticsRisk and Stratification

Basic Processing

Noise filteringArtifacts removalFlexible PipelinesModular StructureEasily Extendable

A flexible online tool that covers all -omics data types4Product

VisualizationMachine LearningAdapts to UserQSAR IntegrationHybrid Cloud On-site HPC

CirSeq: Logical Framework to Study Low Frequency Mutations

Virus-Host Circuitry

Quasi-Species

Other ApplicationsCIRSEQVirus-Host Interaction Insight

Applications5

Advanced Solution To Study Virus Evolution and Virus-Host Circuitry

Go-to-market and Growth StrategyRemote AccessCost-plus Cloud access to the platform for Individual researchers and small teams

ServiceFull service for challenging projects and ongoing analysis streamlining

ReportingExpert input with quality control, visualizations and method recommendations

EducationHybrid online and offline educational projects that can be adapted to specific use cases

Data StorageIntegration with existing repositoriesOr cloud-based database

CustomizationTargeted solutions to address customChallenges using our modular structure

6Business model

Priyal de ZoysaSolutions MarketingThermo Fisher, Genomics England, Seven Bridges, Pfizer

Dr. Claudia CopelandEducations and CommunicationsTulane University, University of Leipzig, USDA-ARS, University of California, San Diego

Shafin KhanCommercialization AdvisorNew Orleans BioInnovation Center, LSU Health Sciences Center

Empowering Researchers through Hands-On Education

Your intuitive web-based platform is perfectly suited for the educational setting and I am excited at the prospect of it being more widely available in this capacity. It has been a great pleasure to work with the Pine Biotech team thus far, and I look forward to our continued collaborations.

Pine Biotech's team has assembled an impressive array of bioinformatics tools and resources into a unified system that enables an amazing variety of end-to-end omics analyses. Their platform has helped our research by enabling us to explore our RNA-seq data in new ways.

As a laboratory scientist, I can personally attest to the dire need for effective educational tools as NGS moves from the esoteric laboratory environment and into the clinical diagnostic setting

Dr. Christian PfallerDr. Chris McGowinDr. Andrew Woodman

7Value

We believe that in the near future, personalized healthcare will become a reality. To enable this transformation, teams of researchers and clinicians need to use and interpret molecular data in a flexible, efficient way

1950 1979

DNA double-helix structure discovered

Genetic code cracked

First DNA sequencing technology developed

Vision: Enable discoveries that enhance individualized treatments

1980 2016

Polymerase chain reaction first developed

FDA approves first personalized therapeutic

Human Genome Project completed2017

First full patient omics sampling

Cost of sequencing drops to $50

Most conditions require personalized approachVision

1. Sampling2. Data Generation3. Analysis

4. Integration

5. Diagnostics and Stratification

Personalized medical treatment selection

8

Molecular Level Precision Diagnostics

Traction to date9

Transforming data analysis for diverse research teams

Multi-million dollar project involving UCSF, BU, CDC, IBM and Haifa UniversityIdentification of Metabolomics Biomarkers in Clinical Trials for Multiple SclerosisComparative Study between Canine and Human Mutations in Bladder Cancer

PHARMA COMPANY

Experienced Team with a Track Record

10Advisory Board

Prof. Raul AndinoProfessor of Immunology,UCSF, Microbiology

Shafin KhanCommercialization Advisor

Prof. Judith FrydmanStanford University, Biology

Operations

Priyal de Zoysa, Ph.D.Solutions Marketing

Dr. Claudia CopelandEducations and Communications

Elia BrodskyCEO

Board of DirectorsProf. Alfred TauberProfessor of Medicine,Boston UniversityProf. Leonid BrodskyDirector, Tauber Bioinformatics Research Center, University of Haifa

Elka NirCarmel Corporation

Ralph LeopoldInterim CFO

Thank you!

Overcoming Technical and Regulatory Challenges

The T-Bio controller assembles and encrypts the data before sending a pipeline package onto the integrated processing unit that can distribute processes between HPC and cloud for processing the data. Depending on the user, the files can either be uploaded directly onto the controller or assembled from a reference database using the client side data warehouseHIPAA Compliant

Controller

Intermediary DataData Storage

HPC Server

External Data StorageExecutable Files

Appendix

AppendixIT-team with technical experience and skillset shared with Tauber Bioinformatics Research Center at Haifa University

Andrey LopatenkoScalable Data and Security Advisor

Recruit Institute of Technology, Walmart Labs, Apple, GoogleAvi TitievskyHPC Developer

Computer Science and Bioinformatics BackgroundMikhail FarkovGP-GPU, HPC developer

Mathematical Modeling and Computer Science Background

Amitha DasariBioinformatics and Visualization

Bioinformatics Background

Dr. Liudmyla VasilencoMathematician

Mathematical Modeling and Algorithm Development

One environment for all types of biological dataAppendix

Genome read clustering Regulation networkAssociation networkeQTLTransponsRNA MappingTranscriptsProtein-coding expressionNon-coding expressionTranspon abundanceGenetic variantsExome mappingEpigenetic signalsTF bindingMS proteomicsMS metabolomicsLigand dockingDrug specificityFunctional enrichment of modulesLibrary of small moleculesScreening, ClusteringCirSeq: virus mutation variantsVirus titer

HostPathogenJunk DNARNA-seq.RNA-editingExome-seq.CHiP-seq.Bisulfite DNAmethylationVirologyStructural biology libraries of moleculesMachine learning Mass-spec

RNA editing