plant and animal genome conference january 11, 2009
TRANSCRIPT
Sequencing of 23 BACs completed prior to November 2008. Sequencing activities distributed amongst 3 different
companies Since that time, 24 BACs have been competed by 454
sequencing. University of Oklahoma Advanced Center for Genome
Technology 53 additional anchored BACs have been verified and are
ready to be sequenced. FISH
Chr 1 - 30 BACs localizedChr 10 - 20 BACs localizedMore details will be presented by Steve Stack this
afternoon.
Library Name /enzyme
Total Number ofclones
Approx number ofclones seqenced
Cloning Vector
HindIII 129024 76000 pBeloBAC11
MboI 50688 25344 pEC BAC I
EcoRI 75000 25344 pIndigoBAC-5
Sheared library N.A. 4800 PUC18-SW
Additional ordered libraries:
S. cheesmannii HindIII pBeloBAC11 100,000 clones >100kb avg.S. pennellii HindIII pBeloBAC11 100,000 clones >100kb avg.
S. lycopersicum Sau3A cosmid 200,000 clones 20 kb avg.S. lycopersicum Sau3A cosmid >100,000 clones > 20 kb avg.
S. lycopersicum sheared fosmid 300,000 clones 40 kb avg.
-OVERGO anchored markers - apx. 800
-BAC-FISH hybridization/localization - 164
300000 100000
5000075000
Continued support and expansion of SGN
FISH localizations End sequencing of remaining 200,000
fosmid clones Phase 3 sequencing of 600 BACs Education and outreach
Breeders ToolboxBioinformatics InternshipSolanaceae Family goes to School
Breeders Toolbox Page on SGN:
http://www.sgn.cornell.edu/breeders/ Currently have links to:
Locus and phenotype databasesMarkers databaseOntology browser
Breeder tools under constructionTutorialOnline QTL analyzerSNP discovery tool
User group for feedbackBreeders from academia and industry13 members to date
Bioinformatics Summer Internship10-week program at SGNUndergraduates (11 since 2005)High School Students (6 since
2005)
2008 Summer Interns
Solanaceae Family Goes to School After school programs (2 – 3 per year) Visits to classrooms (2 per year) Home-schooled students (2 groups per year)
On-site at BTI Bring your Child to Work Day – Cornell and BTI Expanding Your Horizons (held once a year) Develop into a resource for schools and after
schoolsCD
Background information on the Solanaceae
Images, coloring book, word-find puzzle, materials list
Adapt for middle school students in 2009
SOL NewsletterApproximately 400
people on e-mail listAlso available on SGN
Previous issues also available