plant biocontainment
TRANSCRIPT
Agenda
1. Regulation and Oversight
2. Biosafety Levels
3. Containment Strategies
4. Management Practices
5. Plan Information
6. Checklist Review
Regulations and Guidelines
• APHIS Select Agent Regulationso7 CFR 331. 12
• Guidelines: NIH Guidelines (BMBL)oAppendix P
• A Practical Guide to Containment: Plant Biosafety in Research Greenhouses with Transgenic Plants and Microbesohttps://vtechworks.lib.vt.edu/handle/10919/78423
Biological Safety
• BSL2• Lab coat • Eye protection • Gloves
• BSL3• Gowns (solid front) • Eye protection• Gloves
Pathogen Dissemination in Containment
• Inadvertent disseminationoHitchhikersoBreaking protocolo Small intrudersoWastewateroVentilationoCross contamination
Plant Pathogen Transmission: Bacteria
• Not airborne• Moves via water• Mechanically transmitted• Infected seed or plant parts• Soil
Plant Pathogen Transmission: Fungi
• Airborne• Thick-Walled Spores• Moves via water• Mechanically transmitted• Seed Borne
Lab Biocontainment Practices
• PPE• HEPA• Pest Management • Effluent• Movement from registered space
Growth Chamber Advantages
• HEPA filtered• Reduced foot traffic• Equipment maintenance outside of containment
area• Interior is easy to clean• Moveable
Greenhouse Recommendations
Location Structure• Minimize risk to environment
o Timing
• Consider climatic areaoWind Breakso Sump Pumps
• Transport of agentoProcedures for pathogens
• Reinforced heavy frame• Double-pained glass• BSL-3
o Epoxy coatingoWastewater treatmento Security systemo Shower
Loss of Containment
• Weather events, equipment malfunction, human erroroProcedure for containment breaches
• Ways to prevent breacheso TrainingoAlarms (Glass breaks)oWeather Alerts
Shared Spaces
• Prevent contaminationoOne select agent at a timeoDecontaminate work surfaceso State of roomoChange PPE
Checklist
• Areas that have the most departuresoShared space proceduresoDrills and updating plansoPlants accidently exposed to select agentoLocation of PPEoReferring to other plans
APHIS Contact InformationDivision of Agricultural
Select Agents and [email protected]
301- 851-2070
www.selectagents.gov
CDC Contact InformationDivision of Select Agents and Toxins