plant cell biology wouter kohlen 0427705 auxin and fruit set the role solanum lycopersicum auxin...

Plant Cell Biology Wouter Kohlen 0427705

Auxin and Fruit SetThe role Solanum lycopersicum Auxin Response Factor 1

in the initiation of fruit development

http://www.ru.nl/aspx/get.aspx?xdl=/views/kun/xdl/page&ItmIdt=526&SitIdt=3&VarIdt=1


Content

• Introduction

• Gibberellins

• Pollen tube growth

• cDNA-AFLP



Auxin

• Cell elongation• Embryo patterning• Apical dominance• Vascular differentiation• Abscission• Tropisms• Root morphology• Fruit set

Introduction



Auxin

Introduction

Gillapsy;1993



Introduction

Woodward; 2005



Wt 79.8

Introduction



Wt 79

Introduction



Wt

79

Introduction



Gibberellins

Samples

Wt 3 days poll

Wt 3 days unpoll

pathway



Gibberellins



Gibberellins

Samples

Wt day -2

Wt day 5 poll

Wt day 5 unpoll

79.8 day -2

79.8 day 5

To do…

Welke genen?



Pollen tube growth

40x

♀Wt x ♂Wt



Pollen tube growth

100x

♀Wt x ♂Wt



Pollen tube growth

250x

♀Wt x ♂Wt



Pollen tube growth

100x

♀79.8 x ♂79.8



Pollen tube growth

40x

♀79.8 x ♂79.8



Pollen tube growth

100x

♀79.8 x ♂79.8



Pollen tube growth

250x

♀79.8 x ♂79.8



Pollen tube growth

40x

♀79.8 x ♂wt

40x

♀wt x ♂79.8



Pollen tube growth

100x

40x

♀79.8 x ♂wt

40x

♀wt x ♂79.8



Pollen tube growth

250x

40x

♀79.8 x ♂wt

40x

♀wt x ♂79.8



Pollen tube growth

100x

40x

♀79.8 x ♂wt

40x

♀wt x ♂79.8



Pollen tube growth

250x

40x

♀79.8 x ♂wt

40x

♀wt x ♂79.8



Pollen tube growth

100x

40x

♀79.8 x ♂wt

40x

♀wt x ♂79.8



Pollen tube growth

250x

40x

♀79.8 x ♂wt

40x

♀wt x ♂79.8



Pollen tube growth

40x

79.8



Pollen tube growth

40x 100x

250x 400x

79.8



Pollen tube growth

40x 100x

250x 400x

79.8

To be continued



cDNA-AFLP

Samples79.8 D0 emasc. Ovaries79.8 D0 emasc. Walls

79.8 D5 emasc. ovaries79.8 D5 emasc. walls

79.8 D0 not emasc. ovaries79.8 D0 not emasc. walls

79.8 D5 not emasc. ovaries79.8 D5 not emasc. Walls

wt D0 unpoll ovarieswt D0 unpoll walls


wt D5 poll ovarieswt D5 poll walls

166.17 D1 unpoll ovaries166.17 D1 unpoll walls

166.17 D3 unpoll ovaries166.17 D3 unpoll walls

166.17 D1 poll ovaries166.17 D1 poll walls

166.17 D3 poll ovaries166.17 D3 poll walls


wt D3 unpoll ovarieswt D3 poll walls



159 ovaries159 walls



cDNA-AFLP

Order on gelwt D0 unpoll ovarieswt D1 unpoll ovarieswt D3 unpoll ovarieswt D5 unpoll ovarieswt D1 poll ovarieswt D3 poll ovarieswt D5 poll ovarieswt D0 unpoll wallswt D1 unpoll wallswt D3 unpoll wallswt D5 unpoll wallswt D1 poll wallswt D3 Poll wallswt D5 poll walls

166.17 D1 unpoll ovaries166.17 D3 unpoll ovaries166.17 D1 poll ovaries166.17 D3 poll ovaries166.17 D1 unpoll walls166.17 D3 unpoll walls166.17 D1 poll walls166.17 D3 poll walls

79.8 D0 emasc. ovaries79.8 D5 emasc. ovaries79.8 D0 not emasc. ovaries79.8 D5 not emasc. ovaries79.8 D0 emasc. walls79.8 D5 emasc. walls79.8 D0 not emasc. walls79.8 D5 not emasc. Walls

159 ovaries159 walls



cDNA-AFLP

Primer combination 69



cDNA-AFLP

Wt 166.17 79.8 159

Ovules OvulesOvulesWalls

Unpoll

PollPoll Poll

WallsWalls

Unpoll

Poll

E

NE

O W

E

NE


0 1 3 5 0 1 3 5 1 3 1 3 1 3 1 3 0 5 0 5 0 5 0 5 1 3 5 1 3 5

Unpoll Unpoll



Ovules OvulesOvules

cDNA-AFLP

Walls

Unpoll Unpoll Unpoll

PollPoll Poll

WallsWalls

Unpoll

Poll

E

NE

O W

E

NE


Wt 166.17 79.8 159

0 1 3 5 0 1 3 5 1 3 1 3 1 3 1 3 0 5 0 5 0 5 0 5 1 3 5 1 3 5



cDNA-AFLP

Ovaries OvariesOvariesWalls


PollPoll Poll

WallsWalls

Unpoll

Poll

E

NE

O W

E

NE


Wt 166.17 79.8 159

0 1 3 5 0 1 3 5 1 3 1 3 1 3 1 3 0 5 0 5 0 5 0 5 1 3 5 1 3 5



Ovules OvulesOvules

cDNA-AFLP

Walls


PollPoll Poll

WallsWalls

Unpoll

Poll

E

NE

O W

E

NE


Wt 166.17 79.8 159

0 1 3 5 0 1 3 5 1 3 1 3 1 3 1 3 0 5 0 5 0 5 0 5 1 3 5 1 3 5

ARF9


plant cell biology wouter kohlen 0427705 auxin and fruit set the role solanum lycopersicum auxin...

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