poodle ard) and t (s

OW

NER

’S NAM

E: Cathy Prather

DO

G’S N

AME: “Jake”

Happy Top's Jake the G

reatTEST D

ATE: March 25th, 2018

WO

LFINESS

0.0% LO

W

MATER

NAL

HAPLO

TYPEB

1b

PATERN

ALH

APLOTYPE

H1a.8

This certifies the authenticity of Jake’scanine genetic background asdeterm

ined following careful analysis

of more than 200,000 genetic m

arkers.

POO

DLE

(STANDARD

)

100

.0%

Pood

le (Stan

dard

)

Welcom

e to the Em

bark family!

Adam

Boyko, Ph.D

.C

HIEF SC

IENC

E OFFIC

ER

Ryan Boyko

CH

IEF EXEC

UTIV

E OFFIC

ER

“JAKE” HAPPY TOP'S JAKE THE GREAT

DNA Test Report Test Date: March 25th, 2018 embk.me/jake84

BREED MIX

Poodle (Standard): 100.0%

GENETIC STATSWolfiness: 0.0 % LOW Predicted adult weight: 72 lbs Genetic age: 17 human years

TEST DETAILSKit number: EM-5779870 Swab number: 31001801022087

Registration: AKC pr20339905



Fun Fact From 1989 to 1991, John Suterraced a team of Poodles in the Iditarod.Although his teams placed in the backhalf of the pack, he managed to win$2,000 in prize money before retiringhis poodle team. The Iditarod has sincechanged its rules to specify that onlynorthern dog breeds can compete.

POODLE (STANDARD)The Standard Poodle is a popular, water-loving dog used for centuries as a bird dogand popular pet. Poodles were established in Germany by the 15th century. Oddlyenough, they are the national dog breed of France, and they were the most popularbreed of dog in the United States throughout the 1960s and 70s. They're still quitepopular today, owing to their intelligence, trainability, and non-shedding coats.Although well-known for their fancy fur, they’re one of the most intelligent breeds ofdog and require a lot of exercise and stimulation.

RELATED BREEDS

Poodle (Toy) Sibling breed

Poodle(Miniature)

Sibling breed

Maltese Cousin breed

Havanese Cousin breed

Bichon Frise Cousin breed



MATERNAL LINE

Through Jake’s mitochondrial DNA we can trace his mother’s ancestry back to where dogs and people first became friends. Thismap helps you visualize the routes that his ancestors took to your home. Their story is described below the map.

HAPLOGROUP: B1

B1 is the second most common maternal lineage in breeds ofEuropean or American origin. It is the female line of themajority of Golden Retrievers, Basset Hounds, and Shih Tzus,and about half of Beagles, Pekingese and Toy Poodles. Thislineage is also somewhat common among village dogs thatcarry distinct ancestry from these breeds. We know this is aresult of B1 dogs being common amongst the European dogsthat their conquering owners brought around the world,because nowhere on earth is it a very common lineage invillage dogs. It even enables us to trace the path of (human)colonization: Because most Bichons are B1 and Bichons arepopular in Spanish culture, B1 is now fairly common amongvillage dogs in Latin America.

HAPLOTYPE: B1b

Part of the large B1 haplogroup, we see this haplotype invillage dogs across the world, including those from CentralAmerica, the Middle East, South Asia, and the FrenchPolynesian Islands. Among the 31 breed dogs we see it in, wesee it in Poodles, Otterhounds, and Labrador Retrievers. It isalso our most commonly-sampled Golden Retriever haplotype!




PATERNAL LINE

Through Jake’s Y chromosome we can trace his father’s ancestry back to where dogs and people first became friends. This maphelps you visualize the routes that his ancestors took to your home. Their story is described below the map.

HAPLOGROUP: A1a

Some of the wolves that became the original dogs in CentralAsia around 15,000 years ago came from this long anddistinguished line of male dogs. After domestication, theyfollowed their humans from Asia to Europe and then didn't stopthere. They took root in Europe, eventually becoming the dogsthat founded the Vizsla breed 1,000 years ago. The Vizsla is aCentral European hunting dog, and all male Vizslas descendfrom this line. During the Age of Exploration, like their owners,these pooches went by the philosophy, "Have sail, will travel!"From the windy plains of Patagonia to the snug and homeytowns of the American Midwest, the beaches of a Pacificparadise, and the broad expanse of the Australian outback,these dogs followed their masters to the outposts of empires.Whether through good fortune or superior genetics, dogs fromthe A1a lineage traveled the globe and took root across theworld. Now you find village dogs from this line frolicking onPolynesian beaches, hanging out in villages across theAmericas, and scavenging throughout Old World settlements.You can also find this "prince of patrilineages" in breeds as

HAPLOTYPE: H1a.8

Part of the large A1a haplogroup, this very common haplotypeoccurs in village dogs throughout the world (includingsoutheast Asia, which is uncommon for A1a’s). Among the 25breeds we see this haplotype in, it occurs most frequently inLabrador Retriever, Vizsla, and English Springer Spaniel.




TRAITS

E Locus (Mask, Grizzle, Recessive Red) ee

K Locus (Dominant Black) K K

A Locus (Agouti, Sable) a a

D Locus (Dilute, Blue, Fawn) DD

B Locus (Brown, Chocolate, Liver, Red) BB

Coat Color

Furnishings / Improper Coat (RSPO2) FF

Long Haircoat (FGF5) TT

Shedding (MC5R) CT

Curly Coat (KRT71) TT

Body Size - IGF1 II

Body Size - IGF1R GG

Body Size - STC2 TT

Body Size - GHR (E195K) GG

Body Size - GHR (P177L) CC

Other Coat Traits

Body Size

Brachycephaly (BMP3) CC

Natural Bobtail (T) CC

Hind Dewclaws (LMBR1) CC

Blue Eye Color N/N

Altitude Adaptation (EPAS1) GG

Other Body Features

Performance

Genetic Diversity

Inbreeding Coefficient 14% MHC Class II - DLA DRB1No Diversity

MHC Class II - DLA DQA1 and DQB1No Diversity


B B

t



CLINICAL TRAITSThese clinical genetic traits can inform clinical decisions and diagnoses. These traits do not predict a disease state or increasedrisk for disease. We currently assess one clinical trait: Alanine Aminotransferase Activity.

Alanine Aminotransferase Activity result: Low Normal

Happy Top's Jake the Great has one copy of a mutation associated with reduced ALT activity as measured on veterinary bloodchemistry panels. Please inform your veterinarian that Happy Top's Jake the Great has this genotype, as ALT is often used as anindicator of liver health and Happy Top's Jake the Great is likely to have a lower than average resting ALT activity. As such, anincrease in Happy Top's Jake the Great’s ALT activity could be evidence of liver damage, even if it is within normal limits by standardALT reference ranges.

More information on Alanine Aminotransferase Activity: Known to be highly expressed in liver cells, activity levels of alanine aminotransferase, or ALT, is a common value on most bloodchemistry panels and is known to be a sensitive measure of liver health. Dogs with two ancestral G alleles show "normal" activity.Dogs that have one or two copies of the derived A allele may have lower resting levels of ALT activity, known as "low normal". If yourdog's result is "low normal" then when a blood chemistry panel is being interpreted the values that you and your veterinarianconsider "normal" may need to be adjusted. Please note that neither a "normal" nor a "low normal" result for this predicts a diseasestate or increased risk for liver disease. Moreover, this mutation does not associate with increased levels of ALT: If your dog hashigh ALT levels, please consult your veterinarian.




HEALTHGood news! Jake did not test positive for any of the genetic diseases thatEmbark screens for. 0

AT RISK

0CARRIER




OTHER CONDITIONSGood news! Jake tested clear for 8 genetic conditions that are common in his breed.

Von Willebrand Disease Type I (VWF)

Congenital Macrothrombocytopenia (TUBB1 Exon 1, Cavalier King Charles Spaniel Variant)

Progressive Retinal Atrophy - prcd Progressive rod-cone degeneration (PRCD Exon 1)

GM2 Gangliosidosis (HEXB, Poodle Variant)

Degenerative Myelopathy (SOD1A)

Neonatal Encephalopathy with Seizures (NEWS) (ATF2)

Malignant Hyperthermia (RYR1)

Osteochondrodysplasia, Skeletal Dwarfism (SLC13A1)




FULL TEST PANELTo help ensure healthy breeds, every test includes analysis of our full panel of over 160 genetic diseases.

Jake is also clear of 157 other genetic diseases that Embark tests for.


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