possible mirna coregulation of target genes in brain regions by both differential mirna expression...
TRANSCRIPT
Possible miRNA coregulation of target genes in brain regions by both differential miRNA expression and miRNA-targeting-specific promoter methylation
Y-h. TaguchiDept. Phys.Chuo Univ.
Introduction
Epigenetic regulation of gene expression is known to be important nowadays.
e.g.
- Development and Cellular Differentiation- Diseases including Cancer - Aging
Example of Epigenetic regulation of genes
- Transcription Factor Bindings to promoter region- Histon modification- Promoter Methylation- miRNA regulation of target genes.
etc. etc.
InteractionRarely Discussed
Both gene suppression
miRNA
cell nucleus
Gene
Promoter3'UTR
Methylation
cytoplasm
Methylation cell nucleus
miRNA cytoplasm
Previous Research
Su et al (2011, BMC Genomics)miRNAs tended to target the geneswith a low DNA methylation level in their promoter regions Promoter methylation miRNA targeting
Purpose of my study:Promoter methylation is miRNA-targeting-specific or not?
(In other words, promoter methylation is affected by being targeted by individual miRNA or not?)
significantly up/downregulated?(t test, Wilcoxon test, KS test)
MiRaGE :MiRNA Ranking by Gene Expression
miRNAtarget gene
target gene
VS
gene
considered miRNA
MiRaGE Method
Promoter Methylation
hypo/hypermethylated?
My Previous Results
Substantial number of miRNAs have target genes with significantly hyper/hypomethylated promoters
References: - Y-h. Taguchi (2012) IPSJ SIG Tech. Rep. 2012-BIO-31(1) pp.1-6- Y-h. Taguchi, (2013) F1000Research [v1; ref status: approved 1, approved with reservations 1, http://f1000r.es/wv]
This Study
Comparison between miRNA, mRNA and promoter methylation among distinct brain regions (Frontal Cortex [FCTX], Temporal Cortex [TCTX], Cerebellum [CRBLM], Pons [PONS]) in miRNA-centric manner.
150 subject vs 4 regions = 600 samples(We exclude samples without all four regions)
Calculation
- Number of miRNAs whose target genes are significantly(*) up/downregulated among 1921 miRNAs
- Number of miRNAs with significantly (*) hypo/hypermethylated promoters among 1921 miRNAs
- Correlation coefficients of mean ranks between miRNA regulation of target genes and miRNA-targeting-specific promoter methylation
for all six pairs of FCTX, TCTX, CRBLM, and PONS
(*) BH adjusted P
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870
128
221
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