Sensors and Biosensors group

A.P.F. Turner

Aristotele University of Thessaloniki (Greece)

Bucharest University (Romania)

Cranfield University (UK)

Brno University (Cezch Republic)

Cluj-Napoca University (Romania)

•Post-docs

Francesca Bettazzi Simona Scarano

•PhD students

Andrea Ravalli Diego Voccia Stefano Mariani

•Visiting professor

•Erasmus link

Giovanna Marrazza Ilaria PalchettiMaria Minunni

Department of Analytical Chemistry, Faculty of Phar macy, University of Medicine and Pharmacy, IuliuHatieganu”, Cluj-Napoca, Romania Cecilia Cristea, Robert Săndulescu

Nanobioelectronics & Biosensors Group, Catalan Inst itute of Nanotechnology, Campus, Bellaterra, Barcelona, SpainAlfredo de la Escosura-Muñiz, Arben Merkoçi

Department of Chemistry, Faculty of Science, Yazd U niversity, Yazd, IranMohammad Mazloum-Ardakania

Department of Analytical Chemistry, São Paulo State University (UNESP), Araraquara/SP, BrazilHideko Yamanaka

Cranfield Health, Università di Cranfield, Regno UnitoSergey A. Piletsky

Dipartimento di Chimica e Fisica, Università di Bres ciaGuido Faglia

Dipartimento di Chimica e Fisica, Università dell'Aq uilaLuca Lozzi

Department of Physical and Analytical Chemistry, Un iversity of Oviedo, SpainMaria Jesus Lobo Castañón

Department of Physical and Environmental Science, U niversity of Toronto at Scarborough, Toronto, CanadaKagan Kerman

Università “La Sapienza” and Scientific Director, Labo ratorio Antidoping, FMSI, RomaFrancesco Botrè

Istituto di Biochimica delle Proteine, CNRGiuseppe Manco

Collaborations

1. Development and evaluation of sensors and biosensors based on the

coupling of electrochemical, optical and piezoelectric sensors with

enzymes, antibodies, bacteria, whole tissues and nucleic acids

2. Solving analytical problems in clinical chemistry, experimental medicine,

food chemistry and environmental analysis

3. Development of new immobilization chemistries of biomolecules

(enzymes, proteins, nucleic acids, etc.) and analytical procedures suitable

for use with biosensor devices, i.e. flow injection analysis, flow systems,

and microdialysis

Group’s expertise

Autolab

Potentiostat/Galvanostat

(Metrohm, NL)

Palm Sens electrochemical

sensor interfaces (NL)

Home made screen-printed electrodes

Electrochemical instrumentations

Other instrumentations

• Nanodispensing systems

• Spin coater

• Optical microscope

• Themocyclers Biorad for PCR

• Electrophoresis apparatus

• Spectrofluorimeter

• Spectrophotometer

• Fiber optic spectrophotometer-Ocean optic

• ELISA plates reader

• Cary 100 Bio – UV/Vis spectrometer

1. Diaconu, I., Cristea, Hârceagǎ, V., Marrazza, G., Berindan-Neagoe, I., Sǎndulescu, R. Electrochemical immunosensors in breast and ovarian cancer. Clinica Chimica Acta 425, 2013, 128-138.

2. Taleat, Z.; Ravalli, A.; Mazloum-Ardakani, M.; Marrazza, G., CA125 Immunosensor Based on Poly-

Anthranilic Acid Modified Screen-Printed Electrodes. Electroanalysis 2013, 25 (1), 269-277.

3. Ilkhani, H.; Arvand, M.; Ganjali, M. R.; Marrazza, G.; Mascini, M., Nanostructured Screen Printed

Graphite Electrode for the Development of a Novel Electrochemical Genosensor. Electroanalysis

2013, 25 (2), 507-514.

4. Bettazzi, F., Enayati, L., Sánchez, I.C., Motaghed, R., Mascini, M., Palchetti, I., Electrochemical

bioassay for the detection of TNF-α using magnetic beads and disposable screen-printed array of

electrodes. Bioanalysis 2013, 5 (1), 11-19.

5. Bettazzi, F., Hamid-Asl, E., Esposito, C.L., Quintavalle, C.,Formisano N.,Laschi, S.,Catuogno, S.,Iaboni,

M., Marrazza, G., Mascini, M.,Cerchia, L.,De Franciscis, V.,Condorelli, G.,Palchetti, I., Electrochemical

detection of miRNA-222 by use of a magnetic bead-based bioassay. Analytical and Bioanalytical

Chemistry 2013, 405, 1025-34.

6. Scarano, S.; Vestri, A.; Ermini, M. L.; Minunni, M., SPR detection of human hepcidin-25: A critical

approach by immuno- and biomimetic-based biosensing. Biosensors and Bioelectronics 2013, 40

(1), 135-140.

7. Ermini, M. L.; Mariani, S.; Scarano, S.; Minunni, M., Direct detection of genomic DNA by surface

plasmon resonance imaging: An optimized approach. Biosensors and Bioelectronics 2013, 40 (1),

193-199.

8. Ravalli, A.; dos Santos, G. P.; Ferroni, M.; Faglia, G.; Yamanaka, H.; Marrazza, G., New label free

CA125 detection based on gold nanostructured screen-printed electrode. Sensors and Actuators, B:

Chemical 2012.

9. Ilkhani, H.; Mascini, M.; Marrazza, G., The potential affibodies in new cancer marker

immunosensors. 2012; Vol. 109 LNEE, 15-18.

10. Hamidi-Asl, E.; Palchetti, I.; Mascini, M. Introduction of an electrochemical genosensor for

detection of p53 gene via sandwich hybridization method , 2012, Vol. 109 LNEE , pp. 37-41

11. Al-Khafaji, Q. A. M.; Harris, M.; Tombelli, S.; Laschi, S.; Turner, A. P. F.; Mascini, M.; Marrazza, G., An

Electrochemical Immunoassay for HER2 Detection. Electroanalysis 2012, 24 (4), 735-742.

12. Zani, A.; Laschi, S.; Mascini, M.; Marrazza, G., A new electrochemical multiplexed assay for PSA

cancer marker detection. Electroanalysis 2011, 23 (1), 91-99.

13. Scarano, S.,Ermini, M. L., Spiriti, M. M., Mascini, M., Bogani, P., Minunni, M., Simultaneous

detection of transgenic DNA by Surface Plasmon Resonance imaging with potential application to

gene doping detection. Analytical Chemistry 2011, 83 (16), 6245-6253.

14. Ermini, M.L., Scarano, S., Bini, R., Banchelli, M., Berti, D., Mascini, M., Minunni, M., A rational

approach in probe design for nucleic acid-based biosensing. Biosensors and Biolectronics, 2011, 26

(11), 4785-4790.

15. Scarano, S.; Scuffi, C.; Mascini, M.; Minunni, M., Surface Plasmon Resonance imaging-based sensing

for anti-bovine immunoglobulins detection in human milk and serum. Anal Chim Acta 2011, 707 (1–

2), 178-183.

Selected pubblications on clinical diagnostic

(2011 – 2013)Other applications and main research topics

Optical instrumentations

Surface Plasmon Resonance SPR-based instrumentation:

- Fully automated Biacore X™ (GE Healthcare)

- SPR-imaging (Horiba Scientific- France)

Piezoelectric instrumentations (QCM)

• 9.5 MHz AT-Cut quartz crystal (14mm);

Gold evaporated (42.6mm2 area) on

both sides

- Seiko (Japan)

http://www.bioinspired-nano.eu/en/

•COST ACTION

COST TD 1003