power point presentation for science research
DESCRIPTION
TRANSCRIPT
![Page 1: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/1.jpg)
Using Polycaprolactone
for Tissue Engineering
A Research Study by: Satish Bhat
UCONN - STORRS
![Page 2: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/2.jpg)
Biomedical engineering and tissue regeneration are novel fields of research. Lives are lost everyday across the world because of the lack of donor organs. By producing these organs artificially, those people would be able to continue their lives. It was hypothesized that polycaprolactone would be a viable material to use in tissue engineering. This hypothesis was based on previous research that had been done. In this study, many specific analytical tests had to be done to find valid results. For example, a NMR (Nuclear Magnetic Resonance) test was done as well as thermal analysis. During the course of the study, several important results were gained. Thus far, polycaprolactone has been shown as a valid material to be used in tissue engineering. The results of the NMR test showed the presence of stable ions in the polymer as polylactic acid. Polylactic acid is a polymer that is currently being successfully used in tissue engineering. Also, thermal analysis tests showed that the polymer is a thermoplastic and has very similar characteristics to polylactic acid. Results obtained suggest that polycaprolactone is a strong contender for tissue scaffolding. NMR results show the magnetic pulse for polycaprolactone was radiated back out at the same frequency as polylactic acid. Polylactic acid is successfully being used, and because polycaprolactone has similar characteristics; it can possibly be used in scaffolding. Lives can be saved with this research, and polycaprolactone also is better for the environment because of its biodegradability characteristics.
Abstract
![Page 3: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/3.jpg)
Motivation
• Polycaprolactone is a seven-membered polymer with the formula (CH2)5CO2
• Lives are lost because organs die due to old age and disease – donor organs save those people
• Donors are not always available, synthetic organs come into play
Example of Tissue Scaffold
![Page 4: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/4.jpg)
Hypothesis
• It has been hypothesized that polycaprolactone will have similar characteristics to polylactic acid, and will be a viable material for scaffolding
• Prior research has shown that PCL scaffolds “possess mechanical properties within the lower range of…bone, suggesting that they may have the ability to withstand early functional loading.”
How to create grafts for human use
![Page 5: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/5.jpg)
Research Question
• Can polycaprolactone be used in tissue engineering?
• Being investigated by comparing to already used polymer, polylactic acid
• IF similar characteristics, then strong contender for use in scaffolding.
How to create polycaprolactone
![Page 6: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/6.jpg)
Materials
• 0.0620g of Schwartz catalyst (Cp2ZrClH) at room temperature
• 0.0161g of initiator (terepthaldehyde) at room temperature
• 3mL of solvent (toluene) at room temperature
• Argon gas to replace atmosphere• Access to a clock• Liquid nitrogen• 1.0300g of caprolactone at room temperature• NMR test • GPC test• DSC test• Thermal analysis • Mechanical analysis • Stress test• Lab notebook• Ring• Test tube• Hot plate that can spin test tubes• Thermometer• Two 5mL syringes• Analytical balance• DSC pans
Chemical Structure of Caprolactone
![Page 7: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/7.jpg)
Procedure
Add catalyst and initiator Add solvent Replace atmosphere
with argon gas
Heat, spin and freeze test tubeInject caprolactone
Run NMR, GPC, DSC, thermal, and
mechanical analysis
Record Results and analyze
![Page 8: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/8.jpg)
Calculations
Monomer Initiator Catalyst Solvent Temperature
Description Caprolactone terephthaldehyde Cp2ZrClH toluene RT
Ratio 75 1 2 - -
Molecular Weight 114.14 134.13 257.8 - -
Density(g/ml) 1.030 - - - -
Moles 9.024E-03 1.203E-04 2.406E-04 - -
Mass(g) 1.030 0.016 0.060 - -
Volume(ml) 1.000 - - 3.000ml -
This figure shows the calculations used to obtain the amount of each substance.
![Page 9: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/9.jpg)
This figure is the results of the NMR (Nuclear Magnetic Resonance) Test
NMR Results
![Page 10: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/10.jpg)
Structures of Materials
CH2 O C
O
CH2 CH2 CH2 CH2 CH2 OH
a3 b3 c3 d3 e3fh
n
HClCp2ZrO
Og
a1
e1
b1c1
d1
O
O
a
b
c d
e
Polycaprolactone
Caprolactone/Zr = 1/2
Caprolactone
Cp2ZrHCl (Schwartz Reagent)
![Page 11: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/11.jpg)
0 5 10 15 20
Elution Time (Min)
GPC Results
![Page 12: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/12.jpg)
Mechanical Testing Results
0
2,000
4,000
6,000
8,000
10,000
0 5 10 15 20 25Strain
Str
ess,
MP
a
0
1,000
2,000
3,000
0.15 0.16 0.17 0.18 0.19 0.2 0.21 0.22Strain
Stre
ss, M
Pa
modulus
PCL Mn = 218,000
0
1,000
2,000
3,000
4,000
5,000
6,000
7,000
8,000
0 100,000 200,000 300,000 400,000
0
2
4
6
8
50
75
100
125
150
175
200
Mo
du
lus
, MP
a
Mn
Modulus Elongation
Elo
ng
ati
on
, %
![Page 13: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/13.jpg)
DSC ResultsH
ea
t F
low
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
Hea
t Flo
w (
W/g
)
-80 -60 -40 -20 0 20 40 60 80
Temperature (°C)Exo Down Universal V4.2E TA Instruments
-2.0
-1.5
-1.0
-0.5
0.0
0.5
1.0
Hea
t Flo
w (
W/g
)
-80 -60 -40 -20 0 20 40 60 80
Temperature (°C)Exo Down Universal V4.2E TA Instruments
PCL
P(DL)LA
PCL-b-P(DL)LA
PCL-co-P(DL)LA
PCL
P(DL)LA
PCL-b-P(DL)LA
PCL-co-P(DL)LA
Heating Scan Cooling Scan
Temperature (C) Temperature (C)
![Page 14: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/14.jpg)
Conclusions
• NMR Results show that peaks (hydrogen bonds) are in the same location as polylactic acid
• Peaks at 6.8, 4.0, 1.8, 0.5, 0.6, and 0.0 (same locations as polylactic acid)
• Possibly strong contender for tissue scaffolding
Polycaprolactone
![Page 15: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/15.jpg)
Error Analysis
• Further research includes finding more materials that can be used in tissue engineering
• Argon gas was not injected perfectly due to the ring on the test tube not being tight enough
• All measurements are never perfect
Front light mount from PCL
![Page 16: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/16.jpg)
Acknowledgements
Research was assisted by Assistant Professor at UCONN –
Storrs Dr. Alexandru Asandei and Graduate Student Christopher
Simpson.
Research was conducted at the Institute of Material Sciences –
Polymer Building, 97 North Eagleville Road, Storrs, CT.
![Page 17: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/17.jpg)
Citations[1] = "Tissue Engineering | Science/AAAS." Science. Web. 13 Jan. 2011. <http://www.sciencemag.org/content/260/5110/920>.[2] = "Access : Bridging the Gap : Nature." Nature Publishing Group : Science Journals, Jobs, and Information. Web. 13 Jan. 2011. <http://www.nature.com/nature/journal/v433/n7021/full/433019a.html>.[3] = "Book Article." Wiley Online Library. Web. 16 Jan. 2011. <http://onlinelibrary.wiley.com/doi/10.1002/14356007.a05_031/full>.[4] = (177), In Scopus. "ScienceDirect - International Journal of Pharmaceutics : Poly-ε-caprolactone Microspheres and Nanospheres: an Overview." ScienceDirect - Home. Web. 16 Jan. 2011. <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T7W-4C82NG4-1&_user=10&_coverDate=06/18/2004&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=9ebb0ef4eebbbf9125e4194ad60da65d&searchtype=a>.[5] = (5), In Scopus. "ScienceDirect - Process Biochemistry : Biodegradability and Mechanical Properties of Polycaprolactone Composites Encapsulating Phosphate-solubilizing Bacterium Bacillus Sp. PG01." ScienceDirect - Home. Web. 16 Jan. 2011. <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6THB-4MVF4YJ-1&_user=10&_coverDate=04/30/2007&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_searchStrId=1608852147&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=73559a6c1ffbf79377bbad975fa8cba9&searchtype=a>.[6] = (157), In Scopus. "ScienceDirect - Biomaterials : A Novel Degradable Polycaprolactone Networks for Tissue Engineering." ScienceDirect - Home. Web. 16 Jan. 2011. <http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TWB-47DH8T7-C&_user=10&_coverDate=02/28/2003&_rdoc=1&_fmt=high&_orig=search&_origin=search&_sort=d&_docanchor=&view=c&_searchStrId=1608854140&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=7e54ef58465c59b352217964b017717c&searchtype=a>.[7] = William, Jessica M., Adebisi Adewunmi, Rachel M. Schek, Colleen L. Flanagan, and Stephen E. Feinberg. "Bone Tissue Engineering Using Polycaprolactone Scaffolds Fabricated via Selective Laser Sintering." ScienceDirect - Home. MIT - Webs, 8 Nov. 2004. Web. 16 Jan. 2011. <http://web.mit.edu/course/3/3.042/team1_06/reference/modulus/Bone%20tissue%20engineering%20using%20polycaprolactone%20scaffolds%20fabricated%20via%20selective%20laser%20sintering.pdf>.[8] = Kweon H. "Http://www.ncbi.nlm.nih.gov/pubmed/12485798." Pub Med. 24 Feb. 2003. Web. 16 Jan. 2011. http://www.ncbi.nlm.nih.gov/pubmed/12485798[9] = Domingos, Marco. "Polycaprolactone Scaffolds Fabricated via Bioextrusion for Tissue Engineering Applications." Hindawi Publishing Corporation. 11 Oct. 2009. Web. 16 Jan. 2011. <http://www.hindawi.com/journals/ijbm/2009/239643.html>.[10] = "Fabrication of Modified and Functionalized Polycaprolactone Nanofibre Scaffolds for Vascular Tissue Engineering." IOPscience::.. Welcome! Web. 16 Jan. 2011. <http://iopscience.iop.org/0957-4484/16/10/028>.
![Page 18: Power point presentation for science research](https://reader035.vdocument.in/reader035/viewer/2022070302/5482b295b07959290c8b48b0/html5/thumbnails/18.jpg)