Biotechnology-Practical Lab.1 3rd Student-Medical Analysis 2021

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Practical Biotechnology Concept

o Biotechnology is a field of applied biology that involves the use of living

organisms or parts of organisms for practical uses and bioprocesses in

engineering, technology, medicine and other fields requiring bioproducts.

o Modern use similar term includes genetic engineering as well as cell- and

tissue culture technologies.

o , as ‘the appliance of bioscience’, is helping to

diagnose and treat major human diseases.

provide solutions for a cleaner environment.

produce new industrial bioproducts and processes.

o is a rigorous course that

develops problem-solving skills necessary for success doing science at the

bench while exploring in depth the chemistry of biological molecules.

o Knowledge in this area is directly applicable to the biotechnology fields of

pharmaceuticals, environmental processes and remediation, as well as

bioinstrumentation.

Biotechnology-Practical Lab.1 3rd Student-Medical Analysis 2021

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LABORATORY SCHEDULE (Syllabus):

Weeks Lab. Topic

Week 1 Basic Concept and Instruction about Practical Biotechnology Lab.

Week 2 Nucleic Acid (DNA and RNA) Melting Point Determination Lab.

Week 3 Agarose Gel Electrophoresis Methods

Week 4 Polyacrylamide Gel Electrophoresis Analysis

Week 5 Polymerase Chain Reaction(PCR): Its Programs and Protocols of

Running it

Week 6 Finger Printing and Real Time PCR

Week 7 Primers Designs and Their Preparations (Finding Out What

Bioinformatics Can Do for You?)

Week 8 Restriction Enzyme Analysis

Week 9 DNA Ligase Analysis

Week 10 Bacterial Transformation (Competent Cells)

Week 11 Bacterial Conjugation

Week 12 Fluorescent in situ Hybridization Techniques and Southern

Blotting Techniques (Blotting)

Week 13 Western Blotting Technique

Week 14 Northern Blotting Technique

Week 15 Flow Cytometry (FACS) Protocols

Week 16 Gene Analyzer and DNA Sequencing.

Week 17 Chromatography (Paper Chromatography)

Week 18 Column Chromatography and Thin Layer Chromatography

Week 19 Separation of Enzymes and their Determination Using ELISA and

Cobas e400 plus Technique

Week 20 Separation of Vitamins and their Determination the Immunoassay

Analyzer Cobas e411 Technique