Practical outline of Medical Cell Biology for Nepalese Students

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Practical 1 Morphological Structure of Cell 3 hours

Practical 2 Micrometer of the cell 3 hours

Practical 3 Cytochemistry and Cytophysiology 3 hours

Practical 4 Cell Mitosis 3 hours

Practical outline:Practical 1 Morphological Structure of Cell

I. Objective: ⒈The student will master the following skills:

⑴ preparation of cells for examination on the microscope ⑵ staining cells with standard stains⑶ setting up the microscope for contrast enhancement

⒉ The student will be able to:⑴ acquaint with the structure of light microscope⑵ Learn proper handling and become familiar with the use of light microscopes  for biological investigation.⑶ Master the morphological structure character of cell under the light microscope.

II. Content:⒈ The usage of optical microscope⒉ Practise using general optical microscope and observe differient type of tissue cell. ⒊ The main practical slides are provided with:

⑴ human blood smear⑵ toad blood smear⑶ mouse small intestine pillar epidermal cell ⑷ skeleton muscle slide

⒋ Manipulation Make onion epidermal cell temporarily slide

III. Homework: ⒈ Please write down the procedure of the manipulation.⒉ Make drawing of the onion epidermal cell and label the structure.

Practical 2 Micrometer of the cellI. Objective

Master the principle and the usage of micrometerII. Content:

Micrometry the toad red blood cellIII. Homework:

⒈ the calibration of your ocular micrometer (10x,40x)⒉ the radius (a and b) of the toad red blood cell and the nuclear ,use your 40x objective lens.⒊ Calculate the volume of the cell and the nucleoplasmic ratio (NPR)

Practical 3 Cytochemistry and Cytophysiology

I. Objective I: observe some kinds of chemical components and their distribution in cells.II: master methyl green-pyronin staining method to show nucleic acid.II. Content:⒈ Observation of cellular chemistry composition by optical microscope

⑴ glycogen---animal liver smear, PAS reaction

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⑵ acid protein---toad blood smear, acid fixed green staining⑶ alkaline protein---toad blood smear, alkaline acid fixed green staining⑷ DNA--- animal liver smear, fulgen staining⑸ lipid---animal liver smear, Sudan Ⅲ staining⑹ Alkaline phosphoric acid kinase---mouse kidney slide

⒉ Manipulation Make toad blood smear, using methyl green-pyronin method to show DNA and RNA.III. Homework:

⒈ Procedure of the manipulation.⒉ Make drawing of the toad blood smear to show the position of the DNA and RNA.

Practical 4 Cell Mitosis

I. Objective

⒈ Be familiar with the stages of both mitosis and meiosis⒉ Be able to identify the stages of mitosis in both animal and plant cells via the microscopeII. Content:⒈ Observation by the microscope:

⑴ amitosis---paramecium enclosed slide⑵ mitosis of plant cell---the slit slice of onion root tip⑶ mitosis of animal cell---equine ascarid zygote slice

⒉ meosis---photograph of the grasshopper spermaryIII. Homework:

Please make drawing of the mitosis of the onion root tip cell to show the character of the prophase, metaphase, anaphase, telophase.

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