preliminary phytochemical study of four plant …powerpoint presentation author: 2 cool created...

SYAIFUDDIN1

ACEP AKBAR2

BUDI SUTIYA3

SITI HAMIDAH4

PRELIMINARY PHYTOCHEMICAL STUDY OF FOUR PLANT

SPECIES FROM MANGROVE AREA AT KURAU DISTRICT OF

TANAH LAUT SOUTH BORNEO PROVINCE

1,2 FORESTRY RESEARCH INSTITUTE BANJARBARU 3 ,4 LAMBUNG MANGKURAT UNIVERSITY BANJARBARU

INTRODUCTION

1. One of the mangrove forest benefits is as source of medicinal

plants for human life

2. Species of mangrove forest in kurau as medicinal plants:

a. Api-api (Avicennia marina)

b. Bebutah (Excoecaria agallocha)

c. Jeruju (Acanthus ilicifolius)

d. Rambai (Sonneratia caseolaris)

3. Information about the active chemical compounds contained in

four mangrove species is not widely known

4. The Active Chemical Compounds: saponin, flavonoid, quinon,

steroid, triterpenoid, carotenoid, and alkaloid

THE OBJECTIVE

The Objectives of this study is phytochemical testing to

identification of active chemical compounds contained in

api-api, bebutah, jeruju, and rambai

A. Place:

1. Sample was taken in Mangrove forest Kurau

district of Tanah Laut, South Borneo

2. Phytochemical tests conducted in Laboratory of

FMIPA Lambung Mangkurat University Banjarbaru

METHODS

B. Makes of simplicia • Sample: Leaf, Wood, Bark, and Root

• Sample was dried (20°-25°C) and then mashed into powder

C. Phytochemical analysis: Make the test solution for identification saponin, flavonoid, and quinon:

1 gr simplicia included in 100 ml of boiling water for 5 minutes

• Saponin: 10 ml the test solution shaken for 10 seconds, then left for 10

minutes, foam found in the test solution

• Flavonoid: 5 ml the test solution, 5 mg Mg, 1 ml concentrated HCl, half of

the ethanol solution, red-orange

• Quinon: 3 drops of 1 N NaOH solution into 5 ml of test solution, red

Make the filtrate for identification steroid and triterpenoid:

1 gr simplicia, 2 ml methanol, shaken, filtered, the filtrate was taken

• Steroid and triterpenoid: the filtrate, 2 drops C2H4O2, 2 drops H2SO4

Steroid: the test solution to be bluish green

Triterpenoid: the test solution to be purplish red

• Karotenoid: 2 gr simplicia, 5 ml CHCl3, 5 ml C2H5OH, heated for 5 minutes, added water, shaken, red-orange

• Alkaloid: 2 gr simplicia, 5 ml CHCl3, 5 ml NH3, heated for 5 minutes, shaken, filtered, 5 ml H2SO4, shaken, taken the top layer, divided into 3 parts

mayer reagent white sediment,

wagner reagent the test solution to be brown,

dragendorf reagent the test solution to be red

(Source: FMIPA Lambung Mangkurat University Banjarbaru)

1. Api-api (Avicennia marina Forsk)

Potentially

Treating

sunburn

RESULTS

(a) (b) (c) (d)

Part of

Plant

The Active Chemical Compound

Saponin Flavonoid Quinon Steroid Triterpenoid Carotenoid Alkaloid

Leaf - - - + - + +

Wood + - - - - - -

Bark - - - + - - -

Root + - - - - - -

a. Leaf b. Wood c. Bark d. Root

2. Bebutah (Excoecaria agallocha Linn)

Part of

Plant



Leaf - - - + - + -

Wood + - - - - - -

Bark - + + - + - +

Root - - + - + - +

(a) (b) (c) (d)

Potentially

Cure the

swollen


Part of

Plant



Leaf - - - + - + +

Wood - - - + - - -

Bark - - - + - - -

Root - - - + - - -

(a) (b) (c) (d)

3. Jeruju (Acanthus ilicifolius Linn)

Potentially

Sores

recovery


Part of

Plant



Leaf - + + + - + +

Wood + - - - - - -

Bark + + + - + - -

Root - - + + - - -

(a) (b) (c) (d)

4. Rambai (Sonneratia caseolaris Linn)

Potentially

Cold powder

to make the

skin smooth,

tighten the

skin, removes

spots and

pimples on the

skin.


CONCLUSION

Four species of mangrove forest at Kurau Subdistrict

area, in Tanah Laut South Borneo Province namely api-

api, bebutah, jeruju, and rambai contain saponins,

flavonoids, quinone, steroids, triterpenoids, carotenoids,

and alkaloids

Pharmacology, toxicity, and clinical test of four

mangrove species mentioned are strongly recommended

for safely used of medicine by society

preliminary phytochemical study of four plant …powerpoint presentation author: 2 cool created...

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