přenosy jader j. fulka, jr. cbttn a vÚŽv praha fertilization – transformation of meiotic cells...
TRANSCRIPT
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Přenosy jader
J. Fulka, Jr.
CBTTN a VÚŽV
Praha
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Fertilization – transformation of meiotic cells into mitotic cells
Gametes – terminally differentiated and highly specialized
Genomes - (oocyte, sperm) transcriptionally inactive after fertilization
• One cell stage embryo is totipotent
• Fertilization represents RADICAL REPROGRAMMING OF BOTH GENOMES
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A) Sheep
B) Mouse
C) Human
D) Rabbit
E) Bovine
F) Pig
G) Monkey
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Mouse embryo
Human embryo
PB
MPNFPN
FPN
MPN
5-MeC
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Human embryo
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PB
PN
PN MPN
FPN
49% of human 1-cell stage embryos showed abnormal demethylation pattern
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2PB
FP
MP
MP
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Wilmut, I et al. (1997)
Viable offspring derived from fetal and adult mammalian cells.
Nature 385, 810-813
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A
B
AB
AB
AB
AB
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Enukleace
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Přenos jádra
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What do we need??
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Oct4 Cdx2
DAPI merge
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Nanog
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Oct4 DAPI
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DifferentiationβIII tubulin
TROMA 1
DAPI
MF20
+ EB formation
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Problém: 300 oocytů na jednu linii/ 20 – 30 dárkyň
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Pacient ESc linie
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Producing primate embryonic stem cells bysomatic cell nuclear transfer
Nature - doi:10.1038/nature06357
…. potřebujeme vůbec přenosy jader?
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Děkuji za pozornost