!"#$"%&'()*+*,'-+./!"/ 0123'4'56.

(!./!6 789!'!: #7%&"(0& 8;6!()<

(!./!6 =0>?! @%"()$)

*A". !"B)#:C(%D#EF(/56(G(HI.-+. !":H C!$!'G;0 /J$" E")KK!#L/0<:!/$"M30=9)$

A=4#L/0<:!/$"M 'G!6)N&!;0&'G)1;

B.:. 2548

PREPARATION OF MANGO LEAF EXTRACT NIOSOMES

MISS JUTAMAS JIARANAIKULWANITCH

MISS NUTTA TEERANITI

A SPECIAL PROJECT SUBMITTED IN PARTIAL FULFILENT

OF THE REQUIRREMENT FOR

THE BACHELOR DEGREE OF SCIENCE IN PHARMACY

FACULTY OF PHARMACY

MAHIDOL UNIVERSITY

2005

!"#$%"&'()*

("+,-# $%"(."/012' - 314-#5%"5$67891:1;<#

……...……………………………………….

( !"#!$ %&'!(!) *%+,- .,/&0$! 12 )

.................................................................

( !"#!$ %&'(! )*+ ,-, )

…………………………………………….....

( +."/!#-+!0!+12 3+.$+!4+%2 0++1!5+67#+,( )

.!0!+128*95+:;<!

..................................................................

( /!#-+!0!+12 3+. & 8$& =>%165+64&/+ ) .!0!+128*95+:;<!+?$@

…...............................................................

( 3+. .>+A! +&"#!38." ) .!0!+128*95+:;<!+?$@

!"#$%&'

()*+,*-%./01'12.3'45)*5(#$6 .7.&84

!"#$#% & '()*+(,!-.#*/0, 1+23# 4')*/5/ !"!#$%&'()#*+,! : !"#!$% &!!'"(!)*+!,-*, ./.0 /. 12$')(!)#/3!**, 42!5" !/6+"7046***

*#"8 ,5"*#+/59!!: 8$)*#+/53"+;!% :<" ,0'"=/':<,7=

**#"8 ,5"*#+/5 ,.,&>/' 8$)*#+/53"+;!% :<" ,0'"=/':<,7=

***3?.'%."@.*08@.@='AB<C65";, 420'". ,0'"3"+;!%B<C6(!)*03D0'

-.!/.!-01 : .,@4@E:, B:6&,*F4!,., ,GA9=/1 /;#"87H '9"!!)*<'B<H6, 9"!.I"+C6'"JC".J, <./6

B:6&,*F4!,.(Mangiferin) *(K.+"!+I"8/LM.+"!+9/7M1:):C 6 (Mangifera indica Linn.) 0AN:AO0G,P;H".4.2:?=4,+!) ;H".9"!4/9*+1 B=);H".*5QR4D !/+ 9"!3S9T".AR:A /;U2(!)+68%0AN&)*;!A':.,@4@E:V46+"!+9/7M1:):C 6 *WQN4*W,N:(!)+,0G,#"WM.9"!.I"+C6+"!*VH"+?CJ, <./6 @7'3S9T"(X&&/';C"6YM.9"!*;!A':.,@4@E:0AN:AJ=;C49"!9/9*9Z1 D7HB9C 9"!M5H Solulan C24 *(K.+"!*W,N:8 ":86;/ 9"!=7V."7.,@4@E:7H '9"!M5H*8!QN46@[@:&,D.*E4!%8 ":7/.+?6 9"!(!/1*(=AN'.8 ":*VH:VH.V46+"!+9/7;C4DV:/. B=)8 ":*VH:VH.0/R6<:7V46DV:/.B=)+"!+9/7M.!)11 9"!*;!A':.,@4@E:&"9+"!+9/7M1:):C 60I"@7' ,GA9=/1 /;#"87H '9"!!)*<'B<H6 (reverse phase evaporation) 0AN42$<#?:, 45 463"*E=*EA'+ *(K.* =" 1 5/N @:6 @7'M5H Span 60B=)8=4*!+*;4!4= M.4/;!"+C . 1:1 *(K.+"!+!H"6J./6B=)M5HD7*40,=4A*04!%*(K.;/ 0I"=)="' J=9"!07=465ARM<H*<Z. C"9"!M5H Solulan C24 0AN 5 @:=*(4!%*EZ.V46DV:/.0/R6<:7 0I"M<HV."7V46.,@4@E:=7=6 B=)*W,N:8 ":86;/ M<H9/1+?;!;I"!/1 *:QN4.I".,@4@E:0AND7HD(=7V."77H '*8!QN46@[@:&,D.*E4!%8 ":7/.+?6 (High pressure homogenizer) 200 1"!% 3 !41 W1 C"D7H.,@4@E:0AN:AV."74.2#"8:AV."7*=Z9=6 B=)9"!9!)&"';/ 0ANB81 4'?CM.5C 6 270-500 ."@.*:;! *:QN4.I":" ,*8!")<%(!,:"$+"!0ANU?99/9*9Z17H ' ,GA !68*=V*<= B11+:!!U.)+?6 (High performance liquid chromatography) W1 C" Solulan C24 5C '=79"!+?L*+A'9"!9/9*9Z1+"!V46.,@4@E:M.V/R.;4.9"!=7V."7 *:QN4*W,N:8 ":*VH:VH.V46+"!+9/7;C4DV:/.VSR. 0I"M<H(!,:"$+"!0AN9/9*9Z1 (drug entrapped) *W,N:VSR. @7'!H4'=)(!)+,0G,#"WM.9"!9/9*9Z1+"! (% entrapping efficiency) 4'?CM.5C 6 39.45-73.17 @7'.RI"<./9 B=)!H4'=)V469"!9/9*9Z1+"!;C4(!,:"$DV:/.0ANM5H+!H"6J./6 (% drug loading) 4'?CM.5C 6 0.32-6.63 @7'.RI"<./9 B=)*:QN47?J=9"!*W,N:(!,:"$+"!0/R6<:7V46+"!+9/7B=)DV:/.M.+?;!;I"!/10ANM5H+"!+9/7 0AN8 ":*VH:VH.!H4'=) 36.67 @7'.RI"<./9V46DV:/.

W1 C"(!,:"$+"!9/9*9Z1 (!)+,0G,#"WM.9"!9/9*9Z1+"!V46.,@4@E: B=) !H4'=)V469"!9/9*9Z1+"!;C4(!,:"$DV:/.0ANM5H+!H"6J./6 :A8C"*W,N::"90AN+27 *:QN4M5H8 ":*VH:VH.0/R6<:7V46+"!+9/7B=)DV:/.M.!)11

*0C"9/1!H4'=) 5.03 @7'.RI"<./9;C4(!,:";!

!

Abstract

Preparation of Mango Leaf Extract Niosomes

Jutamas Jiaranaikulwanitch, Nutta Teeraniti

Project advisor : Varaporn Junyaprasert*, Nuntavan Bunyapraphatsara**, Uracha Rungsardthong***

*Department of Pharmacy, Faculty of Pharmacy, Mahidol University

** Department of Pharmacognosy, Faculty of Pharmacy, Mahidol University

***National Nanotechnology Center, National Science and Technology Development Agency

Keyword : Niosome, Mangiferin, Reverse phase evaporation, Transdermal delivery

Mangiferin is an active substance in mango leaf (Mangifera indica Linn.) extract having

anti-oxidant, anti-inflammatory and anti-viral activities. The purpose of this study was to develop

niosomes of mango leaf extract to enhance transdermal delivery. Various factors affecting the

drug entrapment of niosomes were investigated. These factors included the use of Solulan C24

as a stabilizer, size reduction by high pressure homogenizer, concentration of extract in lipid and

total concentration of extract and lipid in formulation. Mango leaf extract niosomes were

prepared by a reverse phase evaporation method (45 ºC, 1 h), using 1:1 Span 60 and

cholesterol as wall forming agents and diethyl ether as a solvent. The results indicate that adding

Solulan C24 at 5 mole% of total lipid resulted in the smaller size niosomes with better stability.

Using high pressure homogenizer (200 bars, 3 cycle), the size of the obtained niosomes was

reduced to 270-500 nm with narrow size distribution. When analyzing the entrapped mangiferin

in nisomes by high performance liquid chromatography (HPLC), it was found that niosomes of

Solulan C24 had lower drug lose during size reduction. When the concentration of extract in lipid

increased, the drug entrapped increased. The entrapping efficiency was 39.45-73.17 % by

weight and % drug loading was 0.32-6.63 % weight by weight of total lipid in formulation. In

formulation of 36.67 % weight by weight of lipid of crude extract, the highest drug entrapped,

entrapping efficiency and % drug loading were obtained when using the total concentration of

extract and lipids at 5.03 % weight by volume.