New Patents ”

toxins, chemotherapeutic agents, and the like. The monoclonal antibody secreted by the hybridoma is a valuable reagent for research and diagnosis.

4529713

RADIOACTIVE STILBENE DERIVATIVES IN

RADIOIMMUNOASSAY

Alain Jouquey, Gaeta Touyer, Paris, France as- signed to Roussel Uclaf

Novel radioactive stilbene derivatives marked with iodine 125 or 13 1 of the formula See Patent for Chemical Structure I wherein each A and B are both hydrogen or together form a double bond and R is the remainder of amino acid of the formula R-NH2 or a derivative thereof poss- essing an iodine acceptor group and marked with iodine 125 or 13 1 and their preparation and anti- gens obtained therefrom and a process for pre- paring said antigens.

8502415

NON RADIOACTIVE PROBE AND PROCESS FOR THE

CHARACTERIZATION OF A DTERMINED NUCLEOTIDE

SEQUENCE AND MEANS FOR THE PRODUCTION OF SAID NON

RADIOACTIVE PROBE

Stratis AVRAMEAS, Antoine DANCHIN, Th@r@se TERNYNCK, Fran@ois TRAINCARD, 1, rue Sarasate, F-75015 Paris, France assigned to INSTITUT PASTEUR; IN- STITUT NATIONAL DE LA RECHERCHE SCIENTIFIQUE

Non radioactive probe for the detection by Meir LICHTENSTEIN, Nouria Faze1 hybridization of a determined sequence of SALEHI, Peter Michael POJER, 17 Yeneda nucleotides and comprising as an essential con- Street, North Balwyn, VIC 3104, Australia as- stituent a nucleic acid which itself contains an in- signed to THE ROYAL MELBOURNE

sertate comprising a sequence complementary of said specific sequence of nucleotides. Said probe is characterized in that at least one portion of at least one of the constituent nucleotides of its nucleic acid is replaced by modified bases recognizable by the polymerasis and comprising a group having not more than two atoms of car- bon and, preferably, at least one halogen group.

8502467

PURIFICATION OF CANCER- ASSOCIATED PROTEIN AND

PREPARATION OF ANTIBODY THERETO

Thomas E WEBB, Dorothy E SCHUMM, Mar- garet HANAUSEK-WALASZEK assigned to THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION

It has previously been shown that the serum from patients suffering from a wide range of cancers contains a cancer marker protein having the ability to release RNA from cell nuclei. This cancer marker protein is purified by taking the protein fraction precipitating between 300/, and 50% saturated aqueous ammonium sulfate solu- tion, dialyzing a solution of the protein fraction against TMK buffer, chromatographing the dia- lyzed protein on a molecular sieve and isolating the fraction having a molecular weight of about 60,000; and removing albumin. Injection of the purified protein into rabbits, preparation of serum from blood of the rabbits and absorption of the sera with normal plasma fraction produces an antibody specific to the cancer marker protein and therefore useful in a radioimmunoassay or ELISA test for a wide variety of cancers. Mono- clonal antibody preparations to the protein may be prepared by injecting the purified protein into mice, harvesting the spleen and lymph node cells, fusing these cells with mouse myeloma cells and using the resultant hybridoma cells to produce the monoclonal antibody.

8502604

PREPARATION OF RADIOPHARMACEUTICALS

BASED ON TECHNETIUM-WM/

vi New Patents

HOSPITAL; THE COMMONWEALTH OF AUSTRALIA

radioactive solution substantially free of reducing agent. Technetium ((99m)Tc) in the form of a solution of pertechnetate ions is

A method of reducing a radioactive material comprising, depositing a reducing agent onto an internal surface of a reaction vessel, and con- tatting a solution of the radioactive material with the surface, to thereby provide a reduced

reduced by stannous chloride coated on the in- side of a reaction vessel, to provide a technetium solution free of excess stannous chloride. A kit for preparing technetium-labelled ligands for use as radio-pharmaceutical is also disclosed.

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