preparing and imaging spiders in etoh

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Preparing and imaging spiders in EtOH ike this one) ->

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Preparing and imaging spiders in EtOH. (like this one) ->. Empty specimen(s) into petri dish. Preparing. I prefer to pose spiders over sand. Fill the sand dish with 70% EtOH , enough to cover the specimen completely. (much unlike this photo). - PowerPoint PPT Presentation

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Page 1: Preparing and imaging  spiders in  EtOH

Preparing and imaging spiders in EtOH

(like this one) ->

Page 2: Preparing and imaging  spiders in  EtOH

Preparing

Empty specimen(s) into petri dish

Page 3: Preparing and imaging  spiders in  EtOH

I prefer to pose spiders over sand. Fill the sand dish with 70% EtOH, enough to cover the specimen completely.

Page 4: Preparing and imaging  spiders in  EtOH

Gently transfer the specimen with forceps to the sand dish. You want the specimen completely submerged as to not get air bubbles in your final image.

(much unlike this photo)

Page 5: Preparing and imaging  spiders in  EtOH

Lens & Lighting

The 5D (using 65mm lens) along with the umbrellas positioned as shown in the next slide tend to work best.

So far all of my images have been taken with the 5D/65mm combo.

This is the 5D, with the 65mm lens at a 5.6 aperture.

Page 6: Preparing and imaging  spiders in  EtOH

Lens & Lighting continued

This is my lighting setup. So far it hasn’t failed me!

The Dynalite settings vary depending on the specimen & camera zoon.

Page 7: Preparing and imaging  spiders in  EtOH

And now, the end result…. (big scary spider warning)

Page 8: Preparing and imaging  spiders in  EtOH

Castianeira c.f. variata, dorsal

Page 9: Preparing and imaging  spiders in  EtOH

Castianeira c.f. variata, ventral

Page 10: Preparing and imaging  spiders in  EtOH

Drassylus mexicanus

Page 11: Preparing and imaging  spiders in  EtOH

Habronattus icenoglei

Page 12: Preparing and imaging  spiders in  EtOH

Hololena hola, dorsal

Page 13: Preparing and imaging  spiders in  EtOH

Hololena hola, ventral

Page 14: Preparing and imaging  spiders in  EtOH

Arctosa littoralis, dorsal

Page 15: Preparing and imaging  spiders in  EtOH

Arctosa littoralis, ventral

Page 16: Preparing and imaging  spiders in  EtOH

Synema sp. dorsal

Page 17: Preparing and imaging  spiders in  EtOH

Synema sp. ventral

Page 18: Preparing and imaging  spiders in  EtOH

Xysticus apachecus

Page 19: Preparing and imaging  spiders in  EtOH

Xysticus gosiutus, dorsal

Page 20: Preparing and imaging  spiders in  EtOH

Xysticus gosiutus, ventral