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Allergeenitestaus Maria Nyqvist 10.5.2017

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Page 1: Presentatie support documentMediqSuomiOy.pdf · Presentatie support document Author: Versie 1.0 Created Date: 5/11/2017 1:28:31 PM

Allergeenitestaus

Maria Nyqvist 10.5.2017

Page 2: Presentatie support documentMediqSuomiOy.pdf · Presentatie support document Author: Versie 1.0 Created Date: 5/11/2017 1:28:31 PM

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Page 3: Presentatie support documentMediqSuomiOy.pdf · Presentatie support document Author: Versie 1.0 Created Date: 5/11/2017 1:28:31 PM

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PCR

ELISA

Lateral Flow

LC/MS

Available technologies for food allergen testing (I)

tim

e

eq

uip

men

t

Technologies for Allergen Analysis

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PCR

ELISA

Lateral Flow

LC/MS

Available technologies for food allergen testing (II)

Industrial laboratories, food & beverage

producers, ingredient suppliers, inspection

services, canteens, convenience stores,

consultants

Commercial & official laboratories, research

units, central industrial labs

Commercial & official laboratories, research

units

On-site testing: simple – reliable – rapid

Ready-to-use kits for 14 allergens

No toxic or allergenic substances

No analytical machines required

Technologies for Allergen Analysis

Page 5: Presentatie support documentMediqSuomiOy.pdf · Presentatie support document Author: Versie 1.0 Created Date: 5/11/2017 1:28:31 PM

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RIDASCREEN® and RIDA®QUICK Gliadin products for gluten analysis:

Gluten

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AOAC-RI AOAC-OMA

RIDASCREEN® Gliadin – Official certificates

Gluten

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International Organizations recommend RIDASCREEN® R5 ELISAs (I)

AOECS

Association of

European Coeliac

Societies

recommends

R5 Sandwich ELISA

(Mendez method)

for natural and

heat-processed

foods

R5 ELISA

(competitive ELISA)

for hydrolyzed

gluten

TTB

Alcohol and Tobacco

Tax and Trade

Bureau / USA

TTB Ruling Number

2012-2:

Beer may only be

labeled as gluten-

free if the ‘R5

Mendez

competitive ELISA’

has been used

AOAC

publishes Official

Method of Analysis

Official MethodSM

number 2012.01:

RIDASCREEN®

Gliadin is the

only test kit

fulfilling the

requirements

MEBAK

Central European

Commission for

Brewing Analysis

recommends for

beer

RIDASCREEN®

Gliadin

competitive to

measure the gliadin

concentration

Gluten

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International Organizations recommend RIDASCREEN® R5 ELISAs (II)

AACCI

American Association of Cereal Chemists International

Method 38-50.01 (in process)

R5 Sandwich

RIDASCREEN® Gliadin

Method 38-55.01 (proposed)

R5 competitive

RIDASCREEN® Gliadin competitive

Gluten

Page 9: Presentatie support documentMediqSuomiOy.pdf · Presentatie support document Author: Versie 1.0 Created Date: 5/11/2017 1:28:31 PM

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RIDA®QUICK

Gliadin

R5 antibody detects prolamin of

wheat, rye, barley

No cross reactivity to soya,

buckwheat or other gluten free

cereals

No hook effect

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RIDA®QUIK Gliadin – menetelmäkuvaus

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ELISA –menetelmän hyödyt ja haitat

• Tarkka, rinnakkaismittaukset kaikista näytteistä

• Todellinen kvantitatiivinen tulos

• Monta näytettä yhdellä mittauksella 18/42

• Isoissa näytemäärissä nopeampi kuin pikatestit

• Hitaampi (inkubaatioita väh. 30 min)

• Korkeammat pystytyskustannukset

• Vaatii näyteanalysoinnin uudelleenorganisointia

• Teknisesti haastavampi

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ELISA-määritys

4. Lisää 100µl näyttettä/standardia ja

inkuboi pöydällä 10 min

5. Pese kaivot 3 kertaa 250 µl

pesupuskuria/kaivo.

Lisää 100 µl laimennettua Conjugate-

liuosta ja inkuboi pöydällä 10 min

6. Pese kaivot 3 kertaa 250 µl

pesupuskuria/kaivo.

Lisää 100 µl Chromogen/Substrate-

liuosta ja inkuboi pimeässä 10 min

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7. Lisää 100 µl stop-liuosta

8. Mittaa absorbanssi 450

nm 10 min sisään.

Määrityksen herkkyys vaihtelee eri näytemateriaalien välillä.

Myös eristystapa vaikuttaa herkkyyteen.

ELISA-määritys

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Huomioitavia vaiheita

Näytevalmistus

Näytteiden ja standardien pipetointi

Pesut

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• Hyvin spesifinen, suora määritys allergisoivasta proteiinista

• Vähäiset ristireaktiot

• Soveltuu myös paljon prosessoidun elintarvikkeen analysointiin

• Tehokas eristys erilaisille näytteille ja allergeeneille

• Useamman näytteen seulonta mahdollista

• kvantitatiivinen

• Kaikki parametrit validoitu.

ELISAn edut

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Analytical test kits to ensure gluten-free food

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Kiitos!