Presentation by:Ansuman sahooLab presentation28.03.15

Figure 1. Attenuation mechanism for the regulation of the histidine biosynthetic operon of Salmonella enterica.

Figure 2. Effect of codon substitutions in the his leader region on derepression of his operon transcription.

Figure 3. Histidine operon expression with His5 of the leader substituted by all 64 codons.

Chevance FFV, Le Guyon S, Hughes KT (2014) The Effects of Codon Context on In Vivo Translation Speed. PLoS Genet 10(6): e1004392. doi:10.1371/journal.pgen.1004392http://127.0.0.1:8081/plosgenetics/article?id=info:doi/10.1371/journal.pgen.1004392

Figure 4. Histidine operon expression phenotypes of UCA-NNN and NNN-UCA substitutions at His4-His5 of the His leader peptide.

Chevance FFV, Le Guyon S, Hughes KT (2014) The Effects of Codon Context on In Vivo Translation Speed. PLoS Genet 10(6): e1004392. doi:10.1371/journal.pgen.1004392http://127.0.0.1:8081/plosgenetics/article?id=info:doi/10.1371/journal.pgen.1004392

Figure 5. The His leader with UCA(Ser)-UCA(Ser) at His4-His5 responds to either histidine or serine starvation for loss of attenuation.

Figure 6. Expression of the his operon for specific proline codons at His4-His5 of the His leader on ribosome stalling.

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