presentation - dna origami project
TRANSCRIPT
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Presentation - DNA Origami Project
Beatrix Fulop, Alice Joffard, Norbert Deak and Ioannis
Panagiotas
20/01/2017
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Introduction
I Goal : Design shapes with DNA origami.
I Tools :
I Cadnano
I Maya
I CanDo
I Outline :
I The principle
I Our design
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The principle
I Step 1 : Build the geometric model by reconstructing the
desired shape with rectangles.
I Step 2 : Add one crossover every k = 16 bases.
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The principle
I Step 3 : Design the scaffold strain and add its corresponding
crossovers.
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The principle
I Step 4 : Design the other portions of strains, the staples,
taking into account the crossovers.
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The principle
I Step 5 : Merge adjacent staples when possible.
I Step 6 : Sequence the scaffold, create the staples as its
complements.
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Our design
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Our design
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Our design
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Our design
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Our design
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Sad smiley
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Mario
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References
I Rothemund, P. W. (2006). Folding DNA to create nanoscale
shapes and patterns. Nature, 440(7082), 297-302.
I Rothemund, P. W. Folding DNA to create nanoscale shapes
and patterns Supplementary Notes 1–11.
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