presentation of algebakt project. antibacterial effect of arctic macroalgae species
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Presentation of ALGEBAKT project. Antibacterial effect of arctic macroalgae species. DMV, PhD, Margarita Novoa-Garrido. Alta, Norway, Sept, 6th - 8th, 2010: The 7th Circumpolar Agricultural Conference - PowerPoint PPT PresentationTRANSCRIPT
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Presentation of ALGEBAKT project.Antibacterial effect of arctic macroalgae
speciesDMV, PhD, Margarita Novoa-Garrido
Alta, Norway, Sept, 6th - 8th, 2010: The 7th Circumpolar Agricultural Conference"Circumpolar Agricultural and Land Use Resources - Prospects and Perspectives for Circumpolar Productions and Industries"
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The MABIT-programme is an industrial R&D program for Northern Norway.
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Bioforsk Nord Bodø. Bioproduction and ecosystems along the coast
Coastal ecology - possibilities for commercial exploitation of coastal and marine resources without damaging ecosystems. Our main focus is the algae.
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• Antibiotics: therapy and growth promotion
• Ab-resistance• Antibiotics banned in Europe
as antibiotic growth promoters since 2006
Background for the project
Antibiotic resistance test of P. aeruginosa strain
It was on a short-cut through the hospital kitchens that Albert was first approched by a member of the Antibiotic Resistance.
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Palmaria palmata
Pelvetia canaliculata Alaria esculenta
Foto: Unni Lorentsen
A. nodosum
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ALGINATE
SEAWEED MEAL Sheep in seaweed meal project in
Bioforsk Nord.Foto: H.J. Skulstad
L. hyperboreaA. nodosum
Vegetables in seaweed project in Bioforsk Nord.Foto: I. Øvsthus
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Algae composition
• Dietary fibre (<8% DM)• Protein (5-10 % DM)• Fat (2-4 % DM)• Carbohydrates (45-60 % DM)• Vitamines: A, C, E, B1, B2, B3, B8, B9 • Minerals• Secundary bioactive metabolites
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Macroalgae biologiske effekter
• Antibakterial• Antivirus• Antitumor• Antioxidant• Antiinflammatory• Immune stimulans• Growth stimulant
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Animal health
Diet
Environment
Bacterial population
Immune system
Jejunum and ileum104-106 CFU/mlLactobacilli, coliforms,Streptococci, fusobacteria, clostridia, bacteriodes, bifido bacteria
Stomach up to 102
Lactobacilli, streptococci, yeast
Colon 1010-1012
CFU/mlLactobacilli, bifido bacteria, eubacteria, fusobacteria, coliforms, clostridia, veillonella, yeasts
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Photo: Birger Volden
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Some research results
• Animal husbandry: nr. of E. coli intestine, shedding of E. coli O157 and Salmonella, nr. adherent Lactobacillus sp. Innate immunity and B lymhocyte activity.
• Aquaculture: Antibacterial effect on fish pathogens such as Vibrio sp. and Aeromonas sp.
• Furanones, organohalogens, floratannins, fucoidans, haloperoxidases, alginate
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Aims of the project
• Generate knowledge about bioactive components with antibacterial effect in macroalgae species adapted to the northern Norwegian climate to get a better overview of the potential for developing new products based on their antibacterial property.
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Material and methods
• Bacteria sheep: Lactobacillus sp., Enterococcus faecium, Enterococcus faecalis, different E. coli strains
• Bacteria fish:Vibrio sp., Pseudoalteromonas sp., Aeromonas sp.
• Macroalgae: Four red species and two brown species
• Minimum inhibitory concentration (MIN)• Liquid chromatography-mass spectrometry
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Synergy with other ongoing prosjects• «Natural sources of antioxidants – a necessity
for animal health and welfare and product quality in organic livestock production.»
• The research Council of Norway.• Effekt of feeding macroalgae on the milk
quality of dairy cattle.• Effekt of feeding macroalgae on intestinal
bacterial flora and immune system of sheep.
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Future visions
• Screening• Identification of biologically activ components• Animal experiments
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THANK YOU FOR YOUR ATTENTION!