Presentation of Expression of Certain Enzymes in a Panel of Human Cancer

Cell Lines

By Sara Soth

Background Information

P450 Enzymes Antioxidative Enzymes Drug Metabolism (Enzymes) CYP1A1 and CYP1B1 MnSOD Cancer Cell Lines

Adenocarcinoma Cancer Cell Line

Background Information Continued

P450 Enzymes Includes CYP1A1 and CYP1B1 Catalyze the oxidation of organic

substances.

Antioxidative Enzymes Lack of enzyme causes oxidative

stress, stress will then cause damage or kill cell.

Balance needed.

Background Information Continued

Drug Metabolism (Enzymes)

Equivalent of xenobiotic metabolism. Works with P450 enzymes. Affect on chemotherapy treatment

Background Information Continued

MnSOD Mitochondrial Superoxide Dismutase mRNA Protein

Background Information Continued

Cancer Cell Lines A2780 – Ovarian Cancer

• Carcinoma SKOV-3 – Ovarian Cancer

• Caucasian Adenocarcinoma LOVO and DLD-1– Colorectal Cancer

• Adenocarcinoma MCF-7 and MDA-MB-231– Breast Cancer

• Caucasian Adenocarcinoma HeLa – Cervical Cancer

• Epitheloid Carcinoma C33A – Cervical Cancer

• Carcinoma

Background Information Continued

Methodology RNA isolated by Chomczynski and Sacci

methods. Grown on cell plates Collected at 60% confluent Put in Tris-glycine gel with SDS-page. Transfer to Nitrocellulose Perform immunodetection with Rabbit

polyclonal anti-CYP1B1Ab and MnSOD Ab Densitometric quantification of band

amplification by ImageJ 1.46 software for imaging.

Resources http://www.ncbi.nlm.nih.gov/gene/1543 http://www.ncbi.nlm.nih.gov/gene/1545 http://ghr.nlm.nih.gov/gene/CYP1B1 http://

medical-dictionary.thefreedictionary.com/procarcinogen http://www.aafp.org/afp/2007/0801/p391.pdf http://www.merriam-webster.com/medical/immunostaining http://

cancerres.aacrjournals.org/content/57/14/3026.full.pdf http://

www.ncbi.nlm.nih.gov/pmc/articles/PMC2990475/pdf/nihms236168.pdf

http://www.ncbi.nlm.nih.gov/pubmed/20840865 http://www.oxidativestressresource.org/ http://www.ncbi.nlm.nih.gov/pubmed/2440339