presentations (60 minutes) john perkins (dsm ......john perkins principal scientist december 11,...
TRANSCRIPT
Schedule
Presentations (60 minutes)
John Perkins (DSM) ndash Lignocellulose biomass to ethanol
Michael Leavell (Amyris) ndash HTS and omics platform
Jean-Paul Leonetti (Deinove SA) ndash Deinococcus-based technology
Hans van der Pol (CorbionPurac) ndash Bio-based products portfolio
Panel Discussion (30 minutes)
John Perkins
Principal Scientist
December 11 2013
Bright Science at DSM Development of a Commercial Lignocellulosic Biomass-to-Ethanol Process
BIO Pacific Rim Summit
San Diego CA copy 2013 DSM All rights reserved
Safe harbor statement
This presentation may contain forward-looking statements with respect to DSMrsquos
future (financial) performance and position Such statements are based on current
expectations estimates and projections of DSM and information currently available to
the company DSM cautions readers that such statements involve certain risks and
uncertainties that are difficult to predict and therefore it should be understood that
many factors can cause actual performance and position to differ materially from
these statements DSM has no obligation to update the statements contained in this
presentation unless required by law
The English language version of this document is leading
A more comprehensive discussion of the risk factors affecting DSMrsquos business can be
found in the companyrsquos latest Annual Report which can be found on the companys
corporate website wwwdsmcom
copy 2013 DSM All rights reserved
DSM ndash the Life Sciences and Material Sciences Company
bull Global science-based company active in health nutrition and materials
bull 23500 employees deliver annual net sales of over euro9 billion
bull 10 Workforce in RampD with budget of euro490 million
bull No 1 or 2 in Dow Jones Sustainability Index from 2004 to 2013
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
bull Yeast
bull Ethanol
bull Yeast extracts
bull Vitamins
bull Penicillin
bull Enzymes
bull Citric acid
bull New feedstocks
bull Newimproved biomass
conversion technologies
bull New platform chemicals
bull New biomaterials
bull New biofuels
bull Recombinant enzymes (eg Chymosin)
bull Metabolic engineering (eg Vitamin B2Cephalexin)
bull Biocatalysis (eg Pharma intermediates)
bull Cell culture (eg PerC6TM cell line)
DSMrsquos Biotechnology Platform Centuries of continuous innovations
INDUSTRIAL BIOTECH (21st century)
CLASSICAL BIOTECH
(19th century)
MODERN BIOTECH (20th century)
copy 2013 DSM All rights reserved
Supported by the
European Seventh
Framework Programme
RampD Piloting
Thermostable cellulase mix discovered
Costs reduced by factor of 17
Efficient C5 yeast conversion enabled
Performance of yeast
and enzymes confirmed
DSM Breakthrough in Yeast amp Enzyme Technology (2008-2012)
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
copy 2013 DSM All rights reserved
DSM C5 Yeast Strain Performance Proven at Industrial Scale
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Next Breakthrough in Lowest Cost Ethanol Package Requires Integrated View
EtOH
Cost
copy 2013 DSM All rights reserved Synergy
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Pitch 1 gL NREL dilute acid amp enzymatically
hydrolyzed corn stover 17 dw 5 gL Hac pH 50
bullYield on ethanol produced from
total sugars (C5 amp C6) increased by
8 bullIncreased xylose consumption
Yield
77
Yield
85
Strain I Strain II
A B
copy 2013 DSM All rights reserved
C5 Yeast Strains With Improved Robustness Feedstock with high inhibitor concentration
copy 2013 DSM All rights reserved
Increasing yield by reducing by-product formation and conversion of inhibitors
bull no glycerol production
bull reduction of inhibitory HAc
bull increased ethanol yield (HAc to ethanol)
copy 2013 DSM All rights reserved
bull gpd1 gpd2
bull AAcDH OE
bull GLY DHAP + NADH
DSM has an exclusive license to this technology
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
John Perkins
Principal Scientist
December 11 2013
Bright Science at DSM Development of a Commercial Lignocellulosic Biomass-to-Ethanol Process
BIO Pacific Rim Summit
San Diego CA copy 2013 DSM All rights reserved
Safe harbor statement
This presentation may contain forward-looking statements with respect to DSMrsquos
future (financial) performance and position Such statements are based on current
expectations estimates and projections of DSM and information currently available to
the company DSM cautions readers that such statements involve certain risks and
uncertainties that are difficult to predict and therefore it should be understood that
many factors can cause actual performance and position to differ materially from
these statements DSM has no obligation to update the statements contained in this
presentation unless required by law
The English language version of this document is leading
A more comprehensive discussion of the risk factors affecting DSMrsquos business can be
found in the companyrsquos latest Annual Report which can be found on the companys
corporate website wwwdsmcom
copy 2013 DSM All rights reserved
DSM ndash the Life Sciences and Material Sciences Company
bull Global science-based company active in health nutrition and materials
bull 23500 employees deliver annual net sales of over euro9 billion
bull 10 Workforce in RampD with budget of euro490 million
bull No 1 or 2 in Dow Jones Sustainability Index from 2004 to 2013
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
bull Yeast
bull Ethanol
bull Yeast extracts
bull Vitamins
bull Penicillin
bull Enzymes
bull Citric acid
bull New feedstocks
bull Newimproved biomass
conversion technologies
bull New platform chemicals
bull New biomaterials
bull New biofuels
bull Recombinant enzymes (eg Chymosin)
bull Metabolic engineering (eg Vitamin B2Cephalexin)
bull Biocatalysis (eg Pharma intermediates)
bull Cell culture (eg PerC6TM cell line)
DSMrsquos Biotechnology Platform Centuries of continuous innovations
INDUSTRIAL BIOTECH (21st century)
CLASSICAL BIOTECH
(19th century)
MODERN BIOTECH (20th century)
copy 2013 DSM All rights reserved
Supported by the
European Seventh
Framework Programme
RampD Piloting
Thermostable cellulase mix discovered
Costs reduced by factor of 17
Efficient C5 yeast conversion enabled
Performance of yeast
and enzymes confirmed
DSM Breakthrough in Yeast amp Enzyme Technology (2008-2012)
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
copy 2013 DSM All rights reserved
DSM C5 Yeast Strain Performance Proven at Industrial Scale
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Next Breakthrough in Lowest Cost Ethanol Package Requires Integrated View
EtOH
Cost
copy 2013 DSM All rights reserved Synergy
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Pitch 1 gL NREL dilute acid amp enzymatically
hydrolyzed corn stover 17 dw 5 gL Hac pH 50
bullYield on ethanol produced from
total sugars (C5 amp C6) increased by
8 bullIncreased xylose consumption
Yield
77
Yield
85
Strain I Strain II
A B
copy 2013 DSM All rights reserved
C5 Yeast Strains With Improved Robustness Feedstock with high inhibitor concentration
copy 2013 DSM All rights reserved
Increasing yield by reducing by-product formation and conversion of inhibitors
bull no glycerol production
bull reduction of inhibitory HAc
bull increased ethanol yield (HAc to ethanol)
copy 2013 DSM All rights reserved
bull gpd1 gpd2
bull AAcDH OE
bull GLY DHAP + NADH
DSM has an exclusive license to this technology
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
Safe harbor statement
This presentation may contain forward-looking statements with respect to DSMrsquos
future (financial) performance and position Such statements are based on current
expectations estimates and projections of DSM and information currently available to
the company DSM cautions readers that such statements involve certain risks and
uncertainties that are difficult to predict and therefore it should be understood that
many factors can cause actual performance and position to differ materially from
these statements DSM has no obligation to update the statements contained in this
presentation unless required by law
The English language version of this document is leading
A more comprehensive discussion of the risk factors affecting DSMrsquos business can be
found in the companyrsquos latest Annual Report which can be found on the companys
corporate website wwwdsmcom
copy 2013 DSM All rights reserved
DSM ndash the Life Sciences and Material Sciences Company
bull Global science-based company active in health nutrition and materials
bull 23500 employees deliver annual net sales of over euro9 billion
bull 10 Workforce in RampD with budget of euro490 million
bull No 1 or 2 in Dow Jones Sustainability Index from 2004 to 2013
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
bull Yeast
bull Ethanol
bull Yeast extracts
bull Vitamins
bull Penicillin
bull Enzymes
bull Citric acid
bull New feedstocks
bull Newimproved biomass
conversion technologies
bull New platform chemicals
bull New biomaterials
bull New biofuels
bull Recombinant enzymes (eg Chymosin)
bull Metabolic engineering (eg Vitamin B2Cephalexin)
bull Biocatalysis (eg Pharma intermediates)
bull Cell culture (eg PerC6TM cell line)
DSMrsquos Biotechnology Platform Centuries of continuous innovations
INDUSTRIAL BIOTECH (21st century)
CLASSICAL BIOTECH
(19th century)
MODERN BIOTECH (20th century)
copy 2013 DSM All rights reserved
Supported by the
European Seventh
Framework Programme
RampD Piloting
Thermostable cellulase mix discovered
Costs reduced by factor of 17
Efficient C5 yeast conversion enabled
Performance of yeast
and enzymes confirmed
DSM Breakthrough in Yeast amp Enzyme Technology (2008-2012)
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
copy 2013 DSM All rights reserved
DSM C5 Yeast Strain Performance Proven at Industrial Scale
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Next Breakthrough in Lowest Cost Ethanol Package Requires Integrated View
EtOH
Cost
copy 2013 DSM All rights reserved Synergy
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Pitch 1 gL NREL dilute acid amp enzymatically
hydrolyzed corn stover 17 dw 5 gL Hac pH 50
bullYield on ethanol produced from
total sugars (C5 amp C6) increased by
8 bullIncreased xylose consumption
Yield
77
Yield
85
Strain I Strain II
A B
copy 2013 DSM All rights reserved
C5 Yeast Strains With Improved Robustness Feedstock with high inhibitor concentration
copy 2013 DSM All rights reserved
Increasing yield by reducing by-product formation and conversion of inhibitors
bull no glycerol production
bull reduction of inhibitory HAc
bull increased ethanol yield (HAc to ethanol)
copy 2013 DSM All rights reserved
bull gpd1 gpd2
bull AAcDH OE
bull GLY DHAP + NADH
DSM has an exclusive license to this technology
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
DSM ndash the Life Sciences and Material Sciences Company
bull Global science-based company active in health nutrition and materials
bull 23500 employees deliver annual net sales of over euro9 billion
bull 10 Workforce in RampD with budget of euro490 million
bull No 1 or 2 in Dow Jones Sustainability Index from 2004 to 2013
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
bull Yeast
bull Ethanol
bull Yeast extracts
bull Vitamins
bull Penicillin
bull Enzymes
bull Citric acid
bull New feedstocks
bull Newimproved biomass
conversion technologies
bull New platform chemicals
bull New biomaterials
bull New biofuels
bull Recombinant enzymes (eg Chymosin)
bull Metabolic engineering (eg Vitamin B2Cephalexin)
bull Biocatalysis (eg Pharma intermediates)
bull Cell culture (eg PerC6TM cell line)
DSMrsquos Biotechnology Platform Centuries of continuous innovations
INDUSTRIAL BIOTECH (21st century)
CLASSICAL BIOTECH
(19th century)
MODERN BIOTECH (20th century)
copy 2013 DSM All rights reserved
Supported by the
European Seventh
Framework Programme
RampD Piloting
Thermostable cellulase mix discovered
Costs reduced by factor of 17
Efficient C5 yeast conversion enabled
Performance of yeast
and enzymes confirmed
DSM Breakthrough in Yeast amp Enzyme Technology (2008-2012)
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
copy 2013 DSM All rights reserved
DSM C5 Yeast Strain Performance Proven at Industrial Scale
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Next Breakthrough in Lowest Cost Ethanol Package Requires Integrated View
EtOH
Cost
copy 2013 DSM All rights reserved Synergy
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Pitch 1 gL NREL dilute acid amp enzymatically
hydrolyzed corn stover 17 dw 5 gL Hac pH 50
bullYield on ethanol produced from
total sugars (C5 amp C6) increased by
8 bullIncreased xylose consumption
Yield
77
Yield
85
Strain I Strain II
A B
copy 2013 DSM All rights reserved
C5 Yeast Strains With Improved Robustness Feedstock with high inhibitor concentration
copy 2013 DSM All rights reserved
Increasing yield by reducing by-product formation and conversion of inhibitors
bull no glycerol production
bull reduction of inhibitory HAc
bull increased ethanol yield (HAc to ethanol)
copy 2013 DSM All rights reserved
bull gpd1 gpd2
bull AAcDH OE
bull GLY DHAP + NADH
DSM has an exclusive license to this technology
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
bull Yeast
bull Ethanol
bull Yeast extracts
bull Vitamins
bull Penicillin
bull Enzymes
bull Citric acid
bull New feedstocks
bull Newimproved biomass
conversion technologies
bull New platform chemicals
bull New biomaterials
bull New biofuels
bull Recombinant enzymes (eg Chymosin)
bull Metabolic engineering (eg Vitamin B2Cephalexin)
bull Biocatalysis (eg Pharma intermediates)
bull Cell culture (eg PerC6TM cell line)
DSMrsquos Biotechnology Platform Centuries of continuous innovations
INDUSTRIAL BIOTECH (21st century)
CLASSICAL BIOTECH
(19th century)
MODERN BIOTECH (20th century)
copy 2013 DSM All rights reserved
Supported by the
European Seventh
Framework Programme
RampD Piloting
Thermostable cellulase mix discovered
Costs reduced by factor of 17
Efficient C5 yeast conversion enabled
Performance of yeast
and enzymes confirmed
DSM Breakthrough in Yeast amp Enzyme Technology (2008-2012)
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
copy 2013 DSM All rights reserved
DSM C5 Yeast Strain Performance Proven at Industrial Scale
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Next Breakthrough in Lowest Cost Ethanol Package Requires Integrated View
EtOH
Cost
copy 2013 DSM All rights reserved Synergy
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Pitch 1 gL NREL dilute acid amp enzymatically
hydrolyzed corn stover 17 dw 5 gL Hac pH 50
bullYield on ethanol produced from
total sugars (C5 amp C6) increased by
8 bullIncreased xylose consumption
Yield
77
Yield
85
Strain I Strain II
A B
copy 2013 DSM All rights reserved
C5 Yeast Strains With Improved Robustness Feedstock with high inhibitor concentration
copy 2013 DSM All rights reserved
Increasing yield by reducing by-product formation and conversion of inhibitors
bull no glycerol production
bull reduction of inhibitory HAc
bull increased ethanol yield (HAc to ethanol)
copy 2013 DSM All rights reserved
bull gpd1 gpd2
bull AAcDH OE
bull GLY DHAP + NADH
DSM has an exclusive license to this technology
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
Supported by the
European Seventh
Framework Programme
RampD Piloting
Thermostable cellulase mix discovered
Costs reduced by factor of 17
Efficient C5 yeast conversion enabled
Performance of yeast
and enzymes confirmed
DSM Breakthrough in Yeast amp Enzyme Technology (2008-2012)
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
copy 2013 DSM All rights reserved
DSM C5 Yeast Strain Performance Proven at Industrial Scale
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Next Breakthrough in Lowest Cost Ethanol Package Requires Integrated View
EtOH
Cost
copy 2013 DSM All rights reserved Synergy
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Pitch 1 gL NREL dilute acid amp enzymatically
hydrolyzed corn stover 17 dw 5 gL Hac pH 50
bullYield on ethanol produced from
total sugars (C5 amp C6) increased by
8 bullIncreased xylose consumption
Yield
77
Yield
85
Strain I Strain II
A B
copy 2013 DSM All rights reserved
C5 Yeast Strains With Improved Robustness Feedstock with high inhibitor concentration
copy 2013 DSM All rights reserved
Increasing yield by reducing by-product formation and conversion of inhibitors
bull no glycerol production
bull reduction of inhibitory HAc
bull increased ethanol yield (HAc to ethanol)
copy 2013 DSM All rights reserved
bull gpd1 gpd2
bull AAcDH OE
bull GLY DHAP + NADH
DSM has an exclusive license to this technology
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
copy 2013 DSM All rights reserved
DSM C5 Yeast Strain Performance Proven at Industrial Scale
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Next Breakthrough in Lowest Cost Ethanol Package Requires Integrated View
EtOH
Cost
copy 2013 DSM All rights reserved Synergy
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Pitch 1 gL NREL dilute acid amp enzymatically
hydrolyzed corn stover 17 dw 5 gL Hac pH 50
bullYield on ethanol produced from
total sugars (C5 amp C6) increased by
8 bullIncreased xylose consumption
Yield
77
Yield
85
Strain I Strain II
A B
copy 2013 DSM All rights reserved
C5 Yeast Strains With Improved Robustness Feedstock with high inhibitor concentration
copy 2013 DSM All rights reserved
Increasing yield by reducing by-product formation and conversion of inhibitors
bull no glycerol production
bull reduction of inhibitory HAc
bull increased ethanol yield (HAc to ethanol)
copy 2013 DSM All rights reserved
bull gpd1 gpd2
bull AAcDH OE
bull GLY DHAP + NADH
DSM has an exclusive license to this technology
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Next Breakthrough in Lowest Cost Ethanol Package Requires Integrated View
EtOH
Cost
copy 2013 DSM All rights reserved Synergy
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Pitch 1 gL NREL dilute acid amp enzymatically
hydrolyzed corn stover 17 dw 5 gL Hac pH 50
bullYield on ethanol produced from
total sugars (C5 amp C6) increased by
8 bullIncreased xylose consumption
Yield
77
Yield
85
Strain I Strain II
A B
copy 2013 DSM All rights reserved
C5 Yeast Strains With Improved Robustness Feedstock with high inhibitor concentration
copy 2013 DSM All rights reserved
Increasing yield by reducing by-product formation and conversion of inhibitors
bull no glycerol production
bull reduction of inhibitory HAc
bull increased ethanol yield (HAc to ethanol)
copy 2013 DSM All rights reserved
bull gpd1 gpd2
bull AAcDH OE
bull GLY DHAP + NADH
DSM has an exclusive license to this technology
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Pitch 1 gL NREL dilute acid amp enzymatically
hydrolyzed corn stover 17 dw 5 gL Hac pH 50
bullYield on ethanol produced from
total sugars (C5 amp C6) increased by
8 bullIncreased xylose consumption
Yield
77
Yield
85
Strain I Strain II
A B
copy 2013 DSM All rights reserved
C5 Yeast Strains With Improved Robustness Feedstock with high inhibitor concentration
copy 2013 DSM All rights reserved
Increasing yield by reducing by-product formation and conversion of inhibitors
bull no glycerol production
bull reduction of inhibitory HAc
bull increased ethanol yield (HAc to ethanol)
copy 2013 DSM All rights reserved
bull gpd1 gpd2
bull AAcDH OE
bull GLY DHAP + NADH
DSM has an exclusive license to this technology
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
Increasing yield by reducing by-product formation and conversion of inhibitors
bull no glycerol production
bull reduction of inhibitory HAc
bull increased ethanol yield (HAc to ethanol)
copy 2013 DSM All rights reserved
bull gpd1 gpd2
bull AAcDH OE
bull GLY DHAP + NADH
DSM has an exclusive license to this technology
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
Pathway Optimization
All 1280 pathways were assembled from the separate cassettes
10
28 separate
cassettes
Completed
pathways in
MTP format
bull Transformation host strain
bull Colony picking
bull Screening in liquid media
bull NMR analysis
bull Statistics
copy 2013 DSM All rights reserved
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
2nd International Polysaccharide Conference Aug 29 -Sep 2 2011
Glycerolacetic acid technology
11
Benefits in relation to enzyme cost
Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol
RN1016
Next
strain
Glycerol KO+
acetic acid
External
Glycerol
addition
RN1016
Next
strain
Glycerol KO+
acetic acid
External glycerol
addition
Up to 28 cost
reduction
copy 2013 DSM All rights reserved
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
Optimized Thermostable Enzyme Cocktail
Enzymes
Yeast
CBH1
CBH2
BG
EG-1
EG-IV
copy 2013 DSM All rights reserved
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
Enzyme Cost Reduction
Cost of Enzyme $gallon Ethanol
4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
copy 2013 DSM All rights reserved
int int
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
Biomass
Pretreatment Enzymes
Yeast
Interdependent CBH1
CBH2
BG
EG-1
EG-IV
Summary
EtOH
Cost
2012 2008 2012 Future
$413
lt $ 3
POET Pilot Plant
POET Pilot Plant
Cellulosic bio-ethanol Corn bio-ethanol
We continue to innovate
together with our partners
copy 2013 DSM All rights reserved
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
copy 2013 DSM All rights reserved
Thank you
copy 2013 DSM All rights reserved
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
Back-up Slides
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history
Our vision From land to fossils and ldquobackrdquo to land
9 billion
people
1 billion
people
copy 2013 DSM All rights reserved
1000 3000 2000
Oil c
onsu
mpti
on
Living off the land Living off the land
A brief
moment
in history