prevalence of microplastics in the seal colony of seal rocks victoria australia

The Presence of plastic pollution in the Australian Fur Seal (Arctocephalus pusillus doriferus) colony of Seal Rocks, Victoria, Australia.

Karl S. Jaeger

AbstractThe aim of this project was to determine if there is a presence of plastic pollution in

the Australian Fur Seal (Arctocephalus pusillus doriferus) colony of Seal Rocks, Phillip Island, Victoria, Australia. This project was undertaken in attempt to increase the data available in regards to the presence of plastic pollution in larger vertebrate mammalian species such as the Australian fur seals.

92 samples were taken from the Australian fur seal colony of Seal Rocks in Victoria, Australia and analysed. This was achieved through the use of methods of environmental analysis involving the isolation of plastic from scat samples by drying, sieving, and separation by floating out plastic in a NaCl solution. FT-IR analysis was used to acquire a definitive analysis of what types of plastics were present. Seven samples were discarded due to the moisture content being too high. From 85 samples remaining 14 (16%) contained plastic fragments ranging in sizes of approximately 2-15mm in diameter. FT-IR spectroscopy was then used to determine the types of plastics present. The types plastics indicated were PE, HDPE and Nylon. These plastics all consistent with waste associated with, bottles, plastic packaging and fishing line.

In conclusion it was determined that there is a presence of plastic pollution in the Australian fur seal colony of Seal Rocks consistent with the findings of current research. I believe these results may warrant further monitoring and research of the effects of the presence plastic in the marine food web of not only the seal colonies of Victoria, but also the entire marine ecosystem.

IntroductionSince the observation of microplastic pollution (plastic pieces ≤ 5mm) in the worlds

marine environment in the early 1970s the effects of these plastics on the marine ecosystem has been of interest to scientists. (Carpenter 1972) Research into the presence of plastic pollution in the oceans of the world has revealed that 60-80% of accumulated pollution in the marine environment is plastic waste and that the presence of this plastic presents an ever growing threat to the marine environments. (Andrady 2011) (Wright 2013)

As the threat of plastic pollution in the marine environment increases, concerns have been raised as to the effects of this plastic on the plant and animal life present in the marine ecosystem once it has entered the World’s oceans. (Carson 2013) Studies into the

prevalence of plastic pollution in the marine food web have shown that there is an alarming amount of plastic being found in species such as Marine birds, seals, turtles and filter feeders such as mussels and zoo plankton species. These are just some of the marine life being affected by the presence of plastic in the marine food web. (Andrady 2011) (Carson 2013) (Eriksson. 2013) (Eriksen 2014) (Eriksson 2003)

Materials and Methods

Sample Collection Materials Latex gloves Paper bags (two per scat sample) Large plastic bags (one per paper sample bag) Plastic freezer bags PPE (Wetsuit, wetsuit booties or runners and sun protection - hat, sunscreen etc)

Separation Materials Glass beakers (250mLx5) Fallon tubes x93 Tweezers Standard sieve Stainless steel (Assorted sizes) Filter papers x1 box Aluminium foil Analytical balance Autoclave tape NaCl solution (900g/L) approx 20Ltrs Autoclave bags (140°C+) and boxes Autoclave bags (paper) Pyroneg (disinfectant) Rod stirrer Mortar and pestle PPE (Lab coat, safety glasses, Latex gloves (double gloved) and face masks)

Sample collectionSamples were taken from random areas of the colony during two trips to Seal Rocks,

the first in July (2014) and a second in February (2015). Sampling methods for the detection of plastic pollution vary due to environmental conditions. For this project, sampling of the seals faecal matter was chosen as this method allowed for ease for collection and more importantly, minimum contact with the seal population. (Eriksson 2003) Each sample was placed into a paper bag, then again into a second paper bag. Once this was completed the two bags were placed in a plastic bag and stored in a -80 freezer. Paper bags were used to eliminate the variable of the contamination of the samples by other sources of plastic.

Oven Drying.

All handling of faecal material to be conducted in a class 2 biological cabinet.

The following method was experimental determine after initial trialling of a method involving wet scat material. (Claessen 2013) (Hidalgo-Ruz 2012) (Hollman 2013) The revised drying method decreased the amount of handling time and also dramatically reduced the amount of liquid waste produced.

1. Weigh each scat separately (wet weight) and record the data. This is done to determine the moisture content of the samples. Initial weight minus dry weight.

2. The scat samples will be dried in a drying oven, 75°C for 24hrs. Dried on tray lined with tin foil and each scat to be placed on a separate square piece of tin foil (10x10cm approx) and placed on tray. Scat will be weighed once dried (dry weight).

Separation

1. Once the samples are desiccated (completely void of moisture), place scat into mortar and crush gently so as not to damage ant possible plastic fragments. When scat is completely broken up, place into sieve and gently sieve scat over biological hazard bag.

2. Once scat has been thoroughly sieved, place contents of sieve into 250ml beaker containing 150mLs of NaCl solution (900g/Litre) adjusted. (Hollman 2013) (Claessen 2013)

3. When all samples have been prepared allow to stand, this allows heavier material to settle to the bottom. (Hidalgo-Ruz 2012)

4. Check for visual signs of separation (plastics floating on surface of NaCl solution and solids at the bottom). (Hidalgo-Ruz 2012)

5. Remove any plastics present using tea strainer (small sieve) or tweezers, then place plastic samples in dish (1/sample). (Hidalgo-Ruz 2012) Plastics are then later to be stored in Eppendorf tubes filled with 70% Ethanol.

6. Liquid waste (slurry) is to be filtered first using funnel and filter papers to separate the solid waste from liquid waste. (As per instruction outlined in Lab RA)

7. Liquid waste can then be disposed of via drain after the addition of Pyroneg, solid waste is to be autoclaved and incinerated. (As per instruction outlined in Lab RA)

8. Plastic samples to be stored in Eppendorf tubes in -80 freezer.

Analysis methodsOnce the plastic pieces were isolated, Fourier Transmission Infra Red Spectroscopy

(FT-IR) was used to analyse the viable fragments to determine the type of material present. (Hidalgo-Ruz 2012). A Spectrum 100 FT-IR was used with a range of 650-4000cmˉ¹ used. With settings for Energy = 300, Scan =8 and resolution = 4 (default).

1. Total number of plastic particles found/scat.2. Total plastic size.3. Types of plastic isolated.4. Store as per methods (8)

Results93 samples were taken from the Australian fur seal colony of Seal Rocks in Victoria,

Australia and analysed for the presence of plastic pollution. (Table 2) Seven samples were discarded due to the moisture content being too high. The remaining scat samples displayed and average mass of 59.530±54.370 for wet samples and 26.710±27.070 for dried samples. (Figures 1-2)(Table 3) From the remaining 85 samples 45 plastic like fragments ranging in sizes of approximately 1-15mm in diameter were isolated. (Table 4) Only fragments ≥1mm were kept for analysis, all other material <1mm was discarded. The initial 21 samples contained 45 fragments each having an average number of 2.143±1.797 pieces found.(Table 6) The initial samples displayed assorted shapes, with forms such as filament, spherical, triangular and round pieces present. (Figure 3) On examination the plastic fragments clearly showed signs of weathering with clear indications of abrasion and tearing, with sizes in the range of 1-15mm with an average of 5.313±3.645, with 16% of the initial samples measuring 5mm and 16% measuring 6mm. (Table 7)(Figure 4)

Figure 1: Distribution of weight in wet scats.

Figure 2: Distribution of weight in dry scats.

Figure 3: Examples of confirmed plastics. Samples 1b, 8a and 23a.

2%

12%

12%

12%

16%16%

2%

7%

5%5%

2%5% 2% 2%

% Distribution of size in initial samples

1mm 2mm 3mm 4mm 5mm 6mm 7mm8mm 9mm 10mm 11mm 12mm 13mm 15mm

Figure 4: Percent distribution of size in initial seal scat samples.

FT-IR spectroscopy was then used to determine the types of plastics present. (Appendix, FT-IR results) The total number of confirmed samples to containing plastic fragments was 14 (16%) with 18 pieces being isolated. (Table 8) The plastic fragments confirmed by FT-IR analysis had sizes in the range of 2-12mm in diameter with an average of 6.056±2.532 (Table 9) with 28% of the confirmed pieces measuring 6mm. (Figure 5) The isolated plastics displayed colouring of green/grey, clear and white (Figure 3) with the types plastics identified as Polyethylene (PE) 61%, High density Polyethylene (HDPE) 11% and Nylon 28%. (Table 8)(Figure 6) On examining the plastic fragments the pieces clearly showed signs of weathering with clear indications of abrasion and tearing consistent with server exposure. (Figure 3)

6% 6%

17%

17%28%

11%

6%6% 6%

% distrubition of size of in samples containing confirmed fragments

2mm 3mm 4mm 5mm 6mm8mm 9mm 10mm 12mm

Figure 5: Percent distribution of confirmed plastic fragments.

84%

16%

Percetage of samples contain-ing plastic fragments

Samples containing no plastic Samples containing plastic

Figure 6: Percentage of confirmed sample containing plastic.

DiscussionSeal Rocks is located approximately 2Km off the south-west coast of Phillip Island,

Victoria and is the home to the largest Australian fur seal colony in Australia, with a population of approximately 30,000 seals. With a feeding radius of <200Km within the Bass Straight region, (Figure 7) with some feeding trips recorded up to 1208Km away from the colony, (Kirkwood. 2011) the seals have a large area in which they can come in contact with the plastic pollution effecting the marine food web. (Carson 2013) From the 85 samples collected from the colony 16% of them contained plastic fragments. Analysis of the plastic material isolated from the scat samples revealed the fragments had an average size of 6.056±2.532, the size in this instance is a good indication that the plastics were not ingested by the seals directly as has been document in studies involving marine birds and fish. (Lavers2014) (Tanaka 2013) (Hirai 2011) (Fendall 2009)

Figure 7: Map of location and feeing area. (Kirkwood 2008)

This raises the question as to the origin of the fragments. The ingestion of plastic by marine animals is well documented, with studies on the ingestion of plastic pollution in all its forms by marine animals revealing that plastic pollution has found its way into the marine food web of all marine inhabitants. (Avio 2015) (Lavers 2014) (Eriksson 2003) The seals diet consists of a variety of fish species such as Cephalopods including Arrow Squid, Nototodarus gouldi and Calamari, Redbait (Emmelichtys nitidus), Barracouta (Thyrsites atun), Red Cod (Pseudophycis bachus), Jack Mackerel (Trachurus declivis) and Leatherjackets (Family Monocanthidae). (Kirkwood 2008) (Kirkwood 2008) (Kirkwood. 2011) Studies have shown the accumulation of plastic pollution in pelagic species not unlike those consumed by the seals of

Seal Rocks begins with the smaller organism at the lower end of the food web that these fish regularly feed on. (Bravo Rebolledo 2013) (Baulch 2014) (Boerger 2010) (Setala 2014) The plastic found in these species and the species of prey within the food web is consumed accidently, this has been well documented in studies involving fish, marine bird species and plankton. (Lavers 2014) (Hirai 2011) (Setala 2014) Plastic fragments mistaken for food then pass along the food chain, finally making their way to apex predators such as seals and marine birds. (Bravo Rebolledo 2013) (Lavers 2014)

A number of factors may influence the availability of plastic and the preferences marine animals exhibit towards them. For example, the size and type of materials the fragments are made of can influence the bioavailability of this pollution in the marine ecosystem. The relative density of the plastic can affect the buoyancy of the plastic in seawater allowing the material to be spread throughout the water column. (Table 1) (Lobelle 2011) Other factors that can affect the bioavailability of plastic in the marine environment is the absorption of hydrophobic chemicals to the surface of the plastic.

Table 1: Specific gravities of plastic materials, commonly found in marine pollution. (Eriksson 2003)

This process can change the relative density of materials in the aquatic environment by adding extra material to the particle. (Rochman. 2014) Recent studies have also shown that the accumulation of microbial colonies on the surfaces of plastic and the subsequent formation of biofilm, may contribute to the change of density of these materials and it is this process of changing density that exposes this pollution to the pelagic strata inhabitants. (Lobelle 2011)

The data collected from this project when compared with recent studies is consistent with the indications that the seals are not consuming the plastic as a part of their regular feeding habits, but are consuming the plastic that has been accumulating in their prey species as it passes along the marine food web. (Andrady 2011) (Bravo Rebolledo 2013) (Eriksson 2003) (Setala 2014)

ConclusionPlastic pollution in the marine environment is now of great concern as it presents a

threat not only to marine habitat but to the global ecosystem. (Eriksen 2014) It was the aim of this report to determine if there was a presence of plastic pollution in the Australian Fur seal colony of Seal Rocks, Victoria. The data collect through the analysis of the scat samples taken from the Seal Rocks colony when compared with current research in regards to plastic pollution in marine animals, shows significant indication that there is presence of plastic pollution with in the colony of Seal Rocks.

Current research into the effects of plastic pollution clearly show that plastic pollution in all its forms presents a possible threat to the marine environment. As detailed in this report it is clear there is a presence of plastic pollution in the food web of the Seal Rocks colony. The extent of this pollution can been seen in the data collected by current research and has been reported to be effecting the marine ecosystem in many areas, and it is only through further education of the global governments and their communities that we can begin to develop a better understanding of the increasing problems created by plastic pollution. (Kalogerakis 2015) (Eriksen 2014) Initiatives like the original MARPOL convention and recently the MSFD will make aware the increasing need to develop further research programs to be used to begin to control the increasing effects of plastic pollution in the marine ecosystem, if not in general the world’s ecosystem. (Kalogerakis 2015)

AcknowledgmentsThank you to the staff of the Phillip Island Nature Parks (PINP), with special thanks to Dr Peter Dann and Dr Rebecca Macintosh (PINP). Thank you to the staff of RMIT university, again with special thanks to Associate Professor Mark Osborn (RMIT).

References Andrady, A L. “Microplastic in the marine environment.” Marine pollution bulletin, no. 62 (2011): 1596-1605.

Carpenter, E.J., Smith, K. L. “Plastics on the Sargasso sea surface.” Science, 1972: 1240-1241.

Carson, H. S. “The incidence of plastic ingestion by fish: From the preys prspective.” Marine pollution bulletin, no. 74 (2013): 170-174.

Claessen, M., Van Cauwenberghe, L., Vandegehuchte, M.B., Janssen, C.R. “New techniques for the detection of microplastics in sediments and field collected organisms.” Marine Pollution Bulletin, no. 70 (2013): 227-233.

Eriksen, M., Lebreton L, Carson H, Thiel M, Moore C, Borerro J, Galgan F, Ryan P, Reisser J. “Plastic pollution in the world's oceans: More than 5 trillion plastic pieces weighing over 250,000 tons afloat at sea.” Plos ONE, 2014.

Eriksson, C., Burton, H. “Origins and biological accumulation of small plastic particles in fur seal from Macquarie Island.” Ambio 32, no. 6 (2003): 380-384.

Eriksson., Burton, H., Fitch, S., van den Hoff, J. “Daily accumulation of marine debris on sub Antarctic island beaches.” Marine pollution bulletin, no. 66 (2013): 199-208.

Hidalgo-Ruz, V., Gutow, L., Thompson, R. C., Thiel, M. “Microplastics in the marine environment: A review of methods used for the identification and quantification.” Environmental Science and Technology, no. 46 (2012): 3060-3075.

Hollman, P.C.H., Bouwmeester, H., Peters, R.J.B. Microplastics in the aquatic food chain: Sources, measurements, occurence and potential health risks. Wageningen: RIKILT Wageningen, 2013.

Kalogerakis, N., Arff, J., Banat, I.M., Broch, O.J., Daffonchino, L., Edvardsen, T., Eguiraun, H., Giuliano, L., Handa, A., Lopez-de-Lpina, K, Marigomez, I., Martinez, I., Oie, G., Rojo, F., Skjermo, J., Zanaroli, G., Fva, F. “The role of environmental biotechnology in exploring, exploiting, monitoring, preserving, protecting and decontaminating the marine environment.” New biotechnology 32, no. 1 (2015): 157-167.

Kirkwood R., Lynch, M., Gales, N., Dann, P., Summer, M. “At sea movements and habitat use of adult male Australian fur seals (Arctocephlus pusillus dorifers).” Can. J. Zool \ 84 (2006): 1781-1788.

Kirkwood, R., Hume, F., Hindell, M. “sea temperature variation mediate annual changes in the diet of Australian fur seals in Bass Strait.” Marine ecology progress series 369 (2008): 297-309.

Kirkwood., R., Arnould, J.P.Y. “Foraging trip strategies and habitat use during late pup rearing by lactating Australian fur seals.” Australian Journal of Zoology, no. 59 (2011): 216-226.

Wright, Stephaine L., Thompson, Richard C., Galloway, Tamara S. “The physiacl impacts of microplastic on marine organisms: A review.” Environmental pollution, 2013: 483-492.

Appendix (results)Table 1: Scat samples, initial data

Sample # Foil (g)Foil + Scat Wet

(g) Wet Weight (g)Foil + Scat Dry

(g) Dry Weight (g)

1 3.55 56.48 52.93 38.65 35.1

2 2.68 55.6 52.92 23.01 20.33

3 3.63 39.81 36.18 15.56 11.93

4 2.73 56.9 54.17 23.02 20.29

5 4.31 54.81 50.5 24.69 20.38

6 4.37 31.19 26.82 13.73 9.36

7 4.42 116.39 111.97 7.97 3.55

8 4.51 79.3 74.79 50.97 46.46

9 4.81 270 265.19 120 115.19

10 4.44 190 185.56 130 125.56

11 4.42 93.24 88.82 31.22 26.8

12 4.3 57.7 53.4 27.69 23.39

13 4.4 98.78 94.38 59.23 54.83

14 4.41 53.78 49.37 18.86 14.45

15 4.5 38.78 34.28 24.58 20.08

16 4.46 38.24 33.78 15.27 10.81

17 4.36 179.83 175.47 54.45 50.09

18 4.36 35.91 31.55 23.27 18.91

19 4.26 137.91 133.65 115.41 111.15

20 4.63 40.21 35.58 16.15 11.52

21 4.02 125 120.98 75 70.98

22 4.37 106.41 102.04 27.28 22.91

23 4.5 115.21 110.71 32.35 27.85

24 4.69 66.45 61.76 29.31 24.62

25 3.92 63.37 59.45 37.1 33.18

26 4.37 89.56 85.19 25.78 21.41

27 4.11 37.54 33.43 26.03 21.92

28 4.6 78.4 73.8 55.17 50.57

29 4.41 34.81 30.4 17.75 13.34

30 4.53 33.72 29.19 11.27 6.74

31 4.53 37.56 33.03 18.93 14.4

32 4.41 257 252.59 106.63 102.22

33 3.95 34.27 30.32 12.69 8.74

34 3.78 155 151.22 65.02 61.24

35 4.77 26.1 21.33 13.96 9.19

36 3.98 42.01 38.03 17.86 13.88

37 4.81 20.81 16 11.44 6.63

38 3.66 95.13 91.47 53.83 50.17

39 4 125 121 80.5 76.5

40 3.07 46.75 43.68 22.39 19.32

41 3.94 34.9 30.96 13.89 9.95

42 4.12 115 110.88 42.3 38.18

43 3.93 57.77 53.84 38.69 34.76

44 3.81 16.26 12.45 10.47 6.66

45 3.88 41.14 37.26 22.31 18.43

46 4.01 150 145.99 54.38 50.37

47 3.82 32.44 28.62 12.84 9.02

48 3.89 180 176.11 118.53 114.64

49 3.78 85.27 81.49 47.04 43.26

50 3.77 66.08 62.31 42.11 38.34

51 3.9 31.9 28 25.84 21.94

52 3.87 47.93 44.06 18.06 14.19

53 3.92 25.98 22.06 16.4 12.48

54 3.99 49.51 45.52 28.05 24.06

55 4.02 113.5 109.48 52.01 47.99

56 3.8 30.27 26.47 23.79 19.99

57 3.98 27.49 23.51 13.09 9.11

58 0 0 0 0 0

59 3.83 33.93 30.1 12.95 9.12

60 4.03 33.1 29.07 23.76 19.73

61 3.99 42.27 38.28 18.62 14.63

62 3.74 19.26 15.52 7.74 4

63 3.85 17.89 14.04 15.43 11.58

64 3.98 59.48 55.5 36.03 32.05

65 0 0 0 0 0

66 4.01 52.98 48.97 38.76 34.75

67 3.72 32.73 29.01 21.83 18.11

68 3.9 19.55 15.65 10.22 6.32

69 3.92 17.8 13.88 11.7 7.78

70 4.14 69.63 65.49 44.09 39.95

71 3.88 104.64 100.76 56.63 52.75

72 3.95 11.78 7.83 6.96 3.01

73 4.1 91.66 87.56 36.2 32.1

74 0 0 0 0 0

75 3.98 34.52 30.54 19.78 15.8

76 0 0 0 0 0

77 3.76 25.2 21.44 13.64 9.88

78 0 0 0 0 0

79 3.87 112.77 108.9 56.54 52.67

80 0 0 0 0 0

81 3.95 215 211.05 40.26 36.31

82 3.85 26.4 22.55 11.63 7.78

83 3.75 7.73 3.98 4.44 0.69

84 3.85 62.58 58.73 22.06 18.21

85 3.75 15.08 11.33 5.38 1.63

86 3.89 76.23 72.34 31.29 27.4

87 3.93 65.43 61.5 29.09 25.16

88 3.81 56.08 52.27 19.75 15.94

89 4.08 48.92 44.84 18.77 14.69

90 0 0 0 0 0

91 0 0 0 0 0

92 3.93 77.24 73.31 49.72 45.79

93 3.85 29.4 25.55 10.91 7.06

Table 2: Descriptive Statistics of wet and dry seal scat samples

————— 27/05/2015 10:52:56 AM ————————————————————

Welcome to Minitab, press F1 for help. Descriptive Statistics: Wet Weight (g), Dry Weight (g)

Variable N N* Mean SE Mean StDev Minimum Q1 Median Q3Wet Weight (g) 93 3 59.53 5.64 54.37 0.00 26.01 44.06 83.34Dry Weight (g) 93 0 26.71 2.81 27.07 0.00 9.11 19.32 35.70

Variable MaximumWet Weight (g) 265.19Dry Weight (g) 125.56

Table 3: Number of fragments isolated from scat samples.

Sample# # of fragments Sample#2 # of fragments21 8 25 22 2 32 13 1 33 14 1 34 05 3 36 16 1 38 08 3 40 1

11 2 47 312 0 48 018 1 52 121 2 58 1

22 2 59 023 6 78 2

Table 4: Size of plastic fragments isolated from scat samples.

Sample# Sample size (mm) Sample#2 Sample size (mm)21a 3 21a 81b 4 21b 41c 2 22a 151d 2 22b 81e 2 23a 91f 2 23b 61g 3 23c 41h 2 23d 52a 3 25a 52b 5 25b 33 1 32 44 6 33 6

5a 12 34 05b 9 36 85c 6 38 06 7 40 3

8a 5 47a 138b 10 47b 118c 10 47c 6

11a 5 48 011b 4 52 612 0 58 1218 5 59 0

78a 678b 5

Table 5: Descriptive statistics of number of fragments.

Descriptive Statistics: No. of frags, Sample size (mm), Sample size (mm)2

Variable N N* Mean SE Mean StDev Minimum Q1 Median Q3No. of frags 21 0 2.143 0.392 1.797 1.000 1.000 2.000 2.500

Variable MaximumNo. of frags 8.000

Table 6: Descriptive statistics of size of fragments isolated.

Descriptive Statistics: size mm 2

Variable N N* Mean SE Mean StDev Minimum Q1 Median Q3size mm 2 48 0 5.313 0.526 3.645 0.000 3.000 5.000 7.750

Variable Maximumsize mm 2 15.000

Table 7: FT-IR results, plastics isolated from scat samples.

Sample # Type of plastic Size (mm)1b PE 42a PE 22b PE 84 PE 6

8b PE 1018 PE 5

21a PE 823a HDPE 923b Nylon 623c Nylon 423d Nylon 525a PE 532 PE 433 PE 640 Nylon 347c Nylon 658 HDPE 12

78a PE 6

Table 8: Descriptive statistics of fragment size in confirmed samples.

Descriptive Statistics: type of plastic Size (mm)

Variable N N* Mean SE Mean StDev Minimum Q1 Mediantype of plastic Size (mm) 18 0 6.056 0.597 2.532 2.000 4.000 6.000

Variable Q3 Maximumtype of plastic Size (mm) 8.000 12.000

Results of FT-IR analysis (viable samples only)

cat1b.asc / Spectrum.lst Euclidean Search Hit List 0.729 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.682 A10000 0.674 RT037A TALC GROUND WITH KBR 0.619 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.612 RT404A M3 STARCH, KBR GRIND 0.611 F65540 METHYL ALCOHOL 0.602 RT406A M4 STARCH - KBR GRIND 0.602 RT405A M4 CAT STARCH - KBR GRIND 0.602 AP0053 POLYETHYLENE, CHLORINATED 48% CHLORINE 0.599 AP0050 POLYETHYLENE, CHLORINATED 25% CHLORINE 0.599 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.581 F13170 BENZYL ALCOHOL 0.575 RT448A ALUM ALUMINATES, KBR GRIND 0.572 F49790 L(-)-GLYCERALDEHYDE UNNATURAL FORM 0.571 RT063A POLYPROPYLENE (TALC FILLED) EX B&D FAN HO 0.571 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR

0.563 RT363A ALUMINIUM HYDROXIDE 0.563 RT178A COLLOID 15/40 0.551 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.551 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.548 F28707 B-CYCLODEXTRIN 0.542 F49135 L(-)-GLUCOSE 0.541 F49140 D(+)-GLUCOSE ANHYDROUS 0.539 F14880 BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE 0.537 RT422A STARCOTE 350 OUTTURN R/NO.729155 -MIR KRS-5 0.535 RT579A XEROX FUSER LUBRICANT DOCUTECH (POLYDIMETHYSILOXANE) 0.512 F17531 BROMOMESITYLENE 0.506 RT402A CAT STARCH EX F2 - DRIED, KBR GRIND

Scat2a.sp / Spectrum.lst Euclidean Search Hit List

0.735 RT422A STARCOTE 350 OUTTURN R/NO.729155 -MIR KRS-5 0.656 RT360A COLLIE/COOK OMEGA O/P VARNISH 053038 - M 0.641 RT361A COLLIE PROKOTE O/P VAENISH - MIR ON COATED 0.638 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.623 RM009A KAOLIN CLAY EX CRODA DIAMOND ANVIL CELL 0.616 AP0051 POLYETHYLENE, CHLORINATED 36% CHLORINE 0.601 AP0052 POLYETHYLENE, CHLORINATED 42% CHLORINE 0.601 F51980 HEPTYL-BETA-D-GLUCOPYRANOSIDE 0.599 RT276A COLOURCOAT WHITE ACF699 EX PACIFIC INKS, 0.593 RT446A TONGANAH(CLAY), KBR GRIND 0.593 RT180A PREMIER S.D. EX GEORGIA KAOCIN 0.590 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.588 RT175A ALPHAGLOSS SAMPLE 10114 0.557 RT275A COLOURCOAT WHITE EX PACIFIC INKS, DRIED 0.555 AP0021 METHYL CELLULOSE 0.554 RT278A ACF 129 STANDARD BLACK EX PACIFIC INKS, 0.540 F75083 OCTYL-BETA-D-GLUCOPYRANOSIDE 0.528 RT040A AMMONIUM HYDROGEN TETRABORATE 0.505 F65540 METHYL ALCOHOL 0.493 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR 0.485 F78554 PHENYL-BETA-D-GLUCOPYRANOSIDE 0.481 RT356A SILICONE SIZE EX F6,DRIED @ 105C,SMEARED ON 0.480 F44203 DODECYL-B-D-GLUCOPYRANOSIDE 0.476 F91440 1,1,2-TRICHLOROTRIFLUOROETHANE 0.476 RT266A SILICONE OIL EX TOSHIBA PHOTOCOPIER,SMEA 0.474 F10050 AMYGDALIN 0.467 AP0018 HYDROXYBUTYL METHYL CELLULOSE 8% HYDROXYBUTYL, 20% METHOXYL 0.466 RT219A NALCO F 3054 DEFOAMER, DRIED 105DEG + SME

Scat2b.asc / Spectrum.lst Euclidean Search Hit List 0.666 A10000 0.638 RT448A ALUM ALUMINATES, KBR GRIND 0.619 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.611 AP0053 POLYETHYLENE, CHLORINATED 48% CHLORINE 0.609 RT404A M3 STARCH, KBR GRIND 0.609 RT406A M4 STARCH - KBR GRIND 0.607 RT405A M4 CAT STARCH - KBR GRIND 0.598 RT037A TALC GROUND WITH KBR 0.577 F49790 L(-)-GLYCERALDEHYDE UNNATURAL FORM 0.564 RT178A COLLOID 15/40 0.563 F13170 BENZYL ALCOHOL 0.555 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.544 RT579A XEROX FUSER LUBRICANT DOCUTECH (POLYDIMETHYSILOXANE) 0.538 RT063A POLYPROPYLENE (TALC FILLED) EX B&D FAN HO 0.535 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.535 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.532 RT438A SILICA AR GRADE SIO2.XH2O KBR GRIND

0.530 RT043A ALUM EX F3 : DRIED ON GLASS & GROUND WITH 0.527 RT179A MANILDRA SIZE PRESS STARCH AHF 180 -KBR 0.526 F14880 BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE 0.513 AP0050 POLYETHYLENE, CHLORINATED 25% CHLORINE 0.511 F65540 METHYL ALCOHOL 0.508 RT274A STARCH EX M/V #3 22/4/88 POWDER KBR GRIND 0.508 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.503 RT359A COATES GVM6180 - GRAVURE APPLIED NITROCE 0.501 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR 0.498 RT363A ALUMINIUM HYDROXIDE 0.493 F28707 B-CYCLODEXTRIN

Scat4.asc / Spectrum.lst Euclidean Search Hit List

0.789 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.759 RT037A TALC GROUND WITH KBR 0.652 F65540 METHYL ALCOHOL 0.650 A10000 0.649 RT404A M3 STARCH, KBR GRIND 0.644 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.636 RT405A M4 CAT STARCH - KBR GRIND 0.628 RT406A M4 STARCH - KBR GRIND 0.618 AP0053 POLYETHYLENE, CHLORINATED 48% CHLORINE 0.614 RT422A STARCOTE 350 OUTTURN R/NO.729155 -MIR KRS-5 0.610 F49135 L(-)-GLUCOSE 0.608 RT363A ALUMINIUM HYDROXIDE 0.596 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR 0.596 F49140 D(+)-GLUCOSE ANHYDROUS 0.591 RT402A CAT STARCH EX F2 - DRIED, KBR GRIND 0.589 RT178A COLLOID 15/40 0.588 AP0050 POLYETHYLENE, CHLORINATED 25% CHLORINE 0.587 F28707 B-CYCLODEXTRIN 0.587 F13170 BENZYL ALCOHOL 0.563 F49790 L(-)-GLYCERALDEHYDE UNNATURAL FORM 0.561 RM009A KAOLIN CLAY EX CRODA DIAMOND ANVIL CELL 0.560 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.551 RT063A POLYPROPYLENE (TALC FILLED) EX B&D FAN HO 0.549 F51980 HEPTYL-BETA-D-GLUCOPYRANOSIDE 0.549 RT579A XEROX FUSER LUBRICANT DOCUTECH (POLYDIMETHYSILOXANE) 0.548 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.548 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.547 F14880 BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE

Scat8b.asc / Spectrum.lst Euclidean Search Hit List

0.649 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.643 RT037A TALC GROUND WITH KBR 0.625 A10000 0.618 RT404A M3 STARCH, KBR GRIND 0.613 RT405A M4 CAT STARCH - KBR GRIND 0.609 RT406A M4 STARCH - KBR GRIND 0.600 RT178A COLLOID 15/40 0.591 RT448A ALUM ALUMINATES, KBR GRIND 0.577 AP0053 POLYETHYLENE, CHLORINATED 48% CHLORINE 0.564 F13170 BENZYL ALCOHOL 0.547 F28707 B-CYCLODEXTRIN 0.545 F65540 METHYL ALCOHOL 0.542 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.542 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.538 RT363A ALUMINIUM HYDROXIDE 0.537 F14880 BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE

0.530 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.527 RT359A COATES GVM6180 - GRAVURE APPLIED NITROCE 0.522 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.515 F49790 L(-)-GLYCERALDEHYDE UNNATURAL FORM 0.506 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR 0.498 RT063A POLYPROPYLENE (TALC FILLED) EX B&D FAN HO 0.496 RT579A XEROX FUSER LUBRICANT DOCUTECH (POLYDIMETHYSILOXANE) 0.493 RT179A MANILDRA SIZE PRESS STARCH AHF 180 -KBR 0.490 RT043A ALUM EX F3 : DRIED ON GLASS & GROUND WITH 0.485 RT422A STARCOTE 350 OUTTURN R/NO.729155 -MIR KRS-5 0.485 RT274A STARCH EX M/V #3 22/4/88 POWDER KBR GRIND 0.478 AP0050 POLYETHYLENE, CHLORINATED 25% CHLORINE


0.680 RT037A TALC GROUND WITH KBR 0.660 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.639 A10000 0.619 RT404A M3 STARCH, KBR GRIND 0.609 RT405A M4 CAT STARCH - KBR GRIND 0.601 AP0053 POLYETHYLENE, CHLORINATED 48% CHLORINE 0.595 RT406A M4 STARCH - KBR GRIND 0.578 F13170 BENZYL ALCOHOL 0.568 F28707 B-CYCLODEXTRIN 0.568 F14880 BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE 0.567 RT178A COLLOID 15/40 0.559 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.559 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.551 F65540 METHYL ALCOHOL 0.548 F49135 L(-)-GLUCOSE 0.543 RT363A ALUMINIUM HYDROXIDE 0.527 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR 0.514 RT448A ALUM ALUMINATES, KBR GRIND 0.511 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.510 RT063A POLYPROPYLENE (TALC FILLED) EX B&D FAN HO 0.510 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.510 RT278A ACF 129 STANDARD BLACK EX PACIFIC INKS, 0.507 RT179A MANILDRA SIZE PRESS STARCH AHF 180 -KBR 0.506 F51980 HEPTYL-BETA-D-GLUCOPYRANOSIDE 0.498 AP0050 POLYETHYLENE, CHLORINATED 25% CHLORINE 0.498 RT422A STARCOTE 350 OUTTURN R/NO.729155 -MIR KRS-5 0.495 F49140 D(+)-GLUCOSE ANHYDROUS 0.495 RT359A COATES GVM6180 - GRAVURE APPLIED NITROCE

Scat21a.asc / Spectrum.lst Euclidean Search Hit List

0.778 RT037A TALC GROUND WITH KBR 0.753 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.672 RT404A M3 STARCH, KBR GRIND 0.662 AP0053 POLYETHYLENE, CHLORINATED 48% CHLORINE 0.657 RT405A M4 CAT STARCH - KBR GRIND 0.644 A10000 0.643 RT406A M4 STARCH - KBR GRIND 0.625 F65540 METHYL ALCOHOL 0.603 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.601 RT363A ALUMINIUM HYDROXIDE 0.601 F13170 BENZYL ALCOHOL 0.599 RT178A COLLOID 15/40 0.599 F28707 B-CYCLODEXTRIN 0.585 RT422A STARCOTE 350 OUTTURN R/NO.729155 -MIR KRS-5 0.584 F14880 BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE

0.573 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR 0.571 F49140 D(+)-GLUCOSE ANHYDROUS 0.566 F49135 L(-)-GLUCOSE 0.563 AP0050 POLYETHYLENE, CHLORINATED 25% CHLORINE 0.562 RT402A CAT STARCH EX F2 - DRIED, KBR GRIND 0.557 RT448A ALUM ALUMINATES, KBR GRIND 0.555 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.555 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.553 RT579A XEROX FUSER LUBRICANT DOCUTECH (POLYDIMETHYSILOXANE) 0.552 F49790 L(-)-GLYCERALDEHYDE UNNATURAL FORM 0.539 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.535 RT063A POLYPROPYLENE (TALC FILLED) EX B&D FAN HO 0.515 RM009A KAOLIN CLAY EX CRODA DIAMOND ANVIL CELL

Scat23a.asc / Spectrum.lst Euclidean Search Hit List

0.899 RT366A HDPE-MULTI REFLECTION SURFACE TRANSMISS 0.864 RT118A 2.5% E.V.A MULTI REFLECTION SURFACE TRANS 0.860 RT369A X03(4202) EX EXTHENE, MIR 0.839 RT368A E220 FILM, EX EXTHENE MIR 0.833 RT338A DEFOAMER, DEAIREX 8042 SOLID, SMEARED ON 0.811 RT337A DEFOAMER FOAMASTER SOLID, SMEARED ON DISC 0.810 AP0049 POLYETHYLENE HIGH DENSITY 0.807 RT367A SURLYN MULTI REFLECTION SURFACE TRANSMI 0.806 RT261A SYNTHETIC WAX EX CRODA, CHLOROFORM EXTRA 0.802 AP0038 POLYAMIDE RESIN MELTING PT 95DEG C 0.758 RT362A FOAMASTER DF215 ANTIFOAM EX PETRIE 0.749 RT082A HC WAX 181-185 - HOT MELT ADHESIVE COMPON 0.732 RT227A HDPE SCLAIR 68A EX DUPONT -THIN FILM 0.730 RT270A F2 WET END DEFOAMER, CHLOROFORM EXTRACT 0.725 AP0056 POLYETHYLENE, OXIDIZED ACID NUMBER 15 MG KOH/G 0.724 RT296A HDPE 7541 NCPE 7541 EX NESTE , T-610626, T 0.721 RT297A LDPE NCPE 7530 EX NESTE (TALC FILLED), THIN 0.719 RT419A WAX EX GULLOTINE M3, CHLOROFORM EXTRACT 0.703 RT061A RIEGEL SUPER BARRIER LINER 0.699 RT190A ALKATHENE -SPECACARD A RING -2 FOILS 0.694 RT257A PARAFFIN WAX EX CRODA, CHLOROFORM EXTRACT 0.688 RT036A POLYETHYLENE-TRANSMISSION FILM 0.687 F74752 OCTADECYLAMINE 0.687 RT186A PARACOL WAX, THIN FILM 0.683 RT250A LLDPE DOWLEX 3010 EX DOW CHEM. -THIN FILM 0.669 RT119A E220 FILM EX EXTHENE, TRANSMISSION 0.661 RT451A DEFOAMER LIQUID, KBR GRIND 0.660 RT191A NEWS 2617T -SPECACARD -A RING,2 FOILS

Scat23b.asc / Spectrum.lst Euclidean Search Hit List

0.715 RT428A NOPCOBOND WET STRENGTH RESIN,DRIED 105C,SME 0.658 F22720 CHITIN 0.593 AP0024 NYLON 6 (POLYCARPROLACTAM) 0.584 RT126A NOPCOBOND SWS 25 THIN FILM 0.582 RT557A OVEN DRIED (105C) MILK, MIC. 0.580 RT026A HYDROCHLORIC CASEIN EX PETRIE 0.552 RT382A LATEX BASED SBR - DRIED, KBR GRIND 0.548 AP0025 NYLON 6/6 (POLYHEXAMETHYLENE ADIPAMIDE) 0.540 AP0026 NYLON 6/9 (POLYHEXAMETHYLENE NONANEDIAMIDE) 0.533 RT539A BASE COAT REZOSOL 8233 EX KCA - SMEARED 0.519 F76200 D-PANTHENOL 0.515 F09650 5-AMINO-N-VALERIC ACID 0.512 F73350 NITROGUANIDINE MOISTENED WITH 20%H20 0.511 AP0029 NYLON 6/T (POLYHEXAMETHYLENE TEREPHTHALAMIDE)

0.494 AP0028 NYLON 6/12 (POLYHEXAMETHYLENE DODECANEDIAMIDE) 0.494 RT462A PERCOL 292 1.8.94 CHLOROFORM EXTRACT 0.487 RT540A BASE COAT REZOSOL 8233 EX KCA - CHLOROFORM EXTRACT 0.486 AP0030 NYLON 11 (POLYUNDECANOAMIDE) 0.486 AP0027 NYLON 6/10 (POLYHEXAMETHYLENE SEBACAMIDE) 0.485 F67787 N-METHYLMERCAPTOACETAMIDE 0.484 RT290A NB LOVE CATIONIC STARCH EX F2, DRIED 105C 0.481 F07985 5-AMINOFLUORESCEIN 0.479 RT549A DETAC 3131 EX SHOALHAVEN - SMEAR ON DISK 0.478 RT423A REDOX DYE POWDER -USED ON SCAN RATE PAPER E 0.468 RT447A CAT STARCH - MANILDRA, KBR GRIND 0.461 RT395A THERMALITE GLUE - CHCL3 EXTRACT 0.457 AP0031 NYLON 12 (POLYLAURYLLACTAM) 0.457 F21877 4(5)-CARBOXYFLUORESCEIN

Scat23c.asc / Spectrum.lst Euclidean Search Hit List

0.681 F22720 CHITIN 0.584 RT557A OVEN DRIED (105C) MILK, MIC. 0.575 RT428A NOPCOBOND WET STRENGTH RESIN,DRIED 105C,SME 0.507 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.492 A10000 0.492 RT126A NOPCOBOND SWS 25 THIN FILM 0.481 AP0026 NYLON 6/9 (POLYHEXAMETHYLENE NONANEDIAMIDE) 0.477 RT594A CHEESE SPREAD SINGLES ON KBR DISC 0.476 F28707 B-CYCLODEXTRIN 0.467 AP0027 NYLON 6/10 (POLYHEXAMETHYLENE SEBACAMIDE) 0.462 AP0024 NYLON 6 (POLYCARPROLACTAM) 0.460 RT462A PERCOL 292 1.8.94 CHLOROFORM EXTRACT 0.459 AP0031 NYLON 12 (POLYLAURYLLACTAM) 0.451 AP0030 NYLON 11 (POLYUNDECANOAMIDE) 0.447 F51980 HEPTYL-BETA-D-GLUCOPYRANOSIDE 0.446 RT359A COATES GVM6180 - GRAVURE APPLIED NITROCE 0.440 RT178A COLLOID 15/40 0.439 RT282A LDPE B4524-90 EX NESTE -KBR GRIND ON TGA 0.439 F28705 A-CYCLODEXTRIN 0.437 AP0028 NYLON 6/12 (POLYHEXAMETHYLENE DODECANEDIAMIDE) 0.436 RT395A THERMALITE GLUE - CHCL3 EXTRACT 0.421 RT404A M3 STARCH, KBR GRIND 0.419 F90160 TOMATINE 0.419 F75083 OCTYL-BETA-D-GLUCOPYRANOSIDE 0.416 F76200 D-PANTHENOL 0.414 AP0025 NYLON 6/6 (POLYHEXAMETHYLENE ADIPAMIDE) 0.412 RT406A M4 STARCH - KBR GRIND 0.409 RT405A M4 CAT STARCH - KBR GRIND

Scat23d.dx / Spectrum.lst Euclidean Search Hit List

0.721 RT428A NOPCOBOND WET STRENGTH RESIN,DRIED 105C,SME 0.716 RT026A HYDROCHLORIC CASEIN EX PETRIE 0.695 RT382A LATEX BASED SBR - DRIED, KBR GRIND 0.643 AP0024 NYLON 6 (POLYCARPROLACTAM) 0.621 AP0025 NYLON 6/6 (POLYHEXAMETHYLENE ADIPAMIDE) 0.617 RT557A OVEN DRIED (105C) MILK, MIC. 0.611 RT126A NOPCOBOND SWS 25 THIN FILM 0.600 AP0026 NYLON 6/9 (POLYHEXAMETHYLENE NONANEDIAMIDE) 0.589 F73350 NITROGUANIDINE MOISTENED WITH 20%H20 0.584 AP0028 NYLON 6/12 (POLYHEXAMETHYLENE DODECANEDIAMIDE) 0.575 RT102A CASEIN ,KBR GRIND 0.568 RT462A PERCOL 292 1.8.94 CHLOROFORM EXTRACT 0.558 RT549A DETAC 3131 EX SHOALHAVEN - SMEAR ON DISK

0.551 F76200 D-PANTHENOL 0.546 RT539A BASE COAT REZOSOL 8233 EX KCA - SMEARED 0.541 AP0030 NYLON 11 (POLYUNDECANOAMIDE) 0.540 RT423A REDOX DYE POWDER -USED ON SCAN RATE PAPER E 0.539 AP0027 NYLON 6/10 (POLYHEXAMETHYLENE SEBACAMIDE) 0.535 F22720 CHITIN 0.529 RT540A BASE COAT REZOSOL 8233 EX KCA - CHLOROFORM EXTRACT 0.529 AP0029 NYLON 6/T (POLYHEXAMETHYLENE TEREPHTHALAMIDE) 0.514 F09650 5-AMINO-N-VALERIC ACID 0.512 F07280 8-AMINOCAPRYLIC ACID 0.506 RT447A CAT STARCH - MANILDRA, KBR GRIND 0.505 F21877 4(5)-CARBOXYFLUORESCEIN 0.495 RT395A THERMALITE GLUE - CHCL3 EXTRACT 0.487 RT290A NB LOVE CATIONIC STARCH EX F2, DRIED 105C 0.483 F07985 5-AMINOFLUORESCEIN


0.686 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.665 RT037A TALC GROUND WITH KBR 0.625 RT404A M3 STARCH, KBR GRIND 0.619 RT405A M4 CAT STARCH - KBR GRIND 0.614 A10000 0.612 RT406A M4 STARCH - KBR GRIND 0.582 RT448A ALUM ALUMINATES, KBR GRIND 0.580 RT178A COLLOID 15/40 0.576 F13170 BENZYL ALCOHOL 0.570 F65540 METHYL ALCOHOL 0.567 F28707 B-CYCLODEXTRIN 0.567 AP0053 POLYETHYLENE, CHLORINATED 48% CHLORINE 0.562 RT363A ALUMINIUM HYDROXIDE 0.558 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.558 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.547 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.545 F49790 L(-)-GLYCERALDEHYDE UNNATURAL FORM 0.543 F14880 BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE 0.528 RT579A XEROX FUSER LUBRICANT DOCUTECH (POLYDIMETHYSILOXANE) 0.527 RT359A COATES GVM6180 - GRAVURE APPLIED NITROCE 0.522 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR 0.513 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.513 RT422A STARCOTE 350 OUTTURN R/NO.729155 -MIR KRS-5 0.495 RT063A POLYPROPYLENE (TALC FILLED) EX B&D FAN HO 0.486 RT043A ALUM EX F3 : DRIED ON GLASS & GROUND WITH 0.482 RT402A CAT STARCH EX F2 - DRIED, KBR GRIND 0.480 F72030 SODIUM TETRATHIONATE DIHYDRATE 0.477 F51980 HEPTYL-BETA-D-GLUCOPYRANOSIDE


0.769 RT037A TALC GROUND WITH KBR 0.736 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.694 AP0053 POLYETHYLENE, CHLORINATED 48% CHLORINE 0.667 RT404A M3 STARCH, KBR GRIND 0.659 A10000 0.650 RT405A M4 CAT STARCH - KBR GRIND 0.635 RT406A M4 STARCH - KBR GRIND 0.607 F13170 BENZYL ALCOHOL 0.604 F65540 METHYL ALCOHOL 0.591 RT448A ALUM ALUMINATES, KBR GRIND 0.590 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.588 RT363A ALUMINIUM HYDROXIDE

0.586 RT178A COLLOID 15/40 0.583 F14880 BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE 0.582 F28707 B-CYCLODEXTRIN 0.574 AP0050 POLYETHYLENE, CHLORINATED 25% CHLORINE 0.567 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR 0.564 F49140 D(+)-GLUCOSE ANHYDROUS 0.563 RT422A STARCOTE 350 OUTTURN R/NO.729155 -MIR KRS-5 0.562 F49135 L(-)-GLUCOSE 0.559 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.559 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.556 RT579A XEROX FUSER LUBRICANT DOCUTECH (POLYDIMETHYSILOXANE) 0.546 RT063A POLYPROPYLENE (TALC FILLED) EX B&D FAN HO 0.545 F49790 L(-)-GLYCERALDEHYDE UNNATURAL FORM 0.543 RT402A CAT STARCH EX F2 - DRIED, KBR GRIND 0.541 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.518 RT278A ACF 129 STANDARD BLACK EX PACIFIC INKS,


0.675 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.662 RT037A TALC GROUND WITH KBR 0.654 RT404A M3 STARCH, KBR GRIND 0.645 RT405A M4 CAT STARCH - KBR GRIND 0.639 RT406A M4 STARCH - KBR GRIND 0.632 A10000 0.625 RT178A COLLOID 15/40 0.596 F28707 B-CYCLODEXTRIN 0.584 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.578 F65540 METHYL ALCOHOL 0.577 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.567 F13170 BENZYL ALCOHOL 0.564 RT363A ALUMINIUM HYDROXIDE 0.557 F49790 L(-)-GLYCERALDEHYDE UNNATURAL FORM 0.555 F14880 BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE 0.549 AP0053 POLYETHYLENE, CHLORINATED 48% CHLORINE 0.541 RT448A ALUM ALUMINATES, KBR GRIND 0.537 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.537 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.536 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR 0.532 RT359A COATES GVM6180 - GRAVURE APPLIED NITROCE 0.528 RT402A CAT STARCH EX F2 - DRIED, KBR GRIND 0.522 F51980 HEPTYL-BETA-D-GLUCOPYRANOSIDE 0.518 RT422A STARCOTE 350 OUTTURN R/NO.729155 -MIR KRS-5 0.515 RT063A POLYPROPYLENE (TALC FILLED) EX B&D FAN HO 0.511 AP0050 POLYETHYLENE, CHLORINATED 25% CHLORINE 0.507 RT579A XEROX FUSER LUBRICANT DOCUTECH (POLYDIMETHYSILOXANE) 0.494 F49140 D(+)-GLUCOSE ANHYDROUS


0.572 F22720 CHITIN 0.565 RT428A NOPCOBOND WET STRENGTH RESIN,DRIED 105C,SME 0.558 RT557A OVEN DRIED (105C) MILK, MIC. 0.460 RT359A COATES GVM6180 - GRAVURE APPLIED NITROCE 0.455 RT026A HYDROCHLORIC CASEIN EX PETRIE 0.451 RT126A NOPCOBOND SWS 25 THIN FILM 0.448 F28707 B-CYCLODEXTRIN 0.445 RT524A OPTIC AL BRIGHTENING AGENT (OBA) BLANKOPHOR - DRIED, KBR GRIND 0.445 RT523A BERSET 86325B MELAMINE RESIN

0.438 RT290A NB LOVE CATIONIC STARCH EX F2, DRIED 105C 0.436 F76200 D-PANTHENOL 0.426 RT447A CAT STARCH - MANILDRA, KBR GRIND 0.419 F73350 NITROGUANIDINE MOISTENED WITH 20%H20 0.419 AP0024 NYLON 6 (POLYCARPROLACTAM) 0.417 F70482 NAPHTHOL AS-BI PHOSPHATE 0.416 F07985 5-AMINOFLUORESCEIN 0.409 RT302A ADDITION SPECTRUM BMA COLL SILICA +NB LOVE 0.407 RT423A REDOX DYE POWDER -USED ON SCAN RATE PAPER E 0.407 F28705 A-CYCLODEXTRIN 0.405 RT178A COLLOID 15/40 0.403 RT539A BASE COAT REZOSOL 8233 EX KCA - SMEARED 0.402 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.402 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.402 F21877 4(5)-CARBOXYFLUORESCEIN 0.400 F72030 SODIUM TETRATHIONATE DIHYDRATE 0.399 AP0026 NYLON 6/9 (POLYHEXAMETHYLENE NONANEDIAMIDE) 0.398 AP0025 NYLON 6/6 (POLYHEXAMETHYLENE ADIPAMIDE) 0.398 RT462A PERCOL 292 1.8.94 CHLOROFORM EXTRACT

Scat47c.asc / Spectrum.lst Euclidean Search Hit List

0.705 RT557A OVEN DRIED (105C) MILK, MIC. 0.676 F22720 CHITIN 0.601 RT428A NOPCOBOND WET STRENGTH RESIN,DRIED 105C,SME 0.525 RT126A NOPCOBOND SWS 25 THIN FILM 0.520 F51980 HEPTYL-BETA-D-GLUCOPYRANOSIDE 0.519 F76200 D-PANTHENOL 0.514 F28707 B-CYCLODEXTRIN 0.481 F75083 OCTYL-BETA-D-GLUCOPYRANOSIDE 0.478 F28705 A-CYCLODEXTRIN 0.461 RT524A OPTIC AL BRIGHTENING AGENT (OBA) BLANKOPHOR - DRIED, KBR GRIND 0.461 RT523A BERSET 86325B MELAMINE RESIN 0.459 RM009A KAOLIN CLAY EX CRODA DIAMOND ANVIL CELL 0.459 RT359A COATES GVM6180 - GRAVURE APPLIED NITROCE 0.456 F88561 THIODIETHYLENE GLYCOL 0.445 RT178A COLLOID 15/40 0.444 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.437 F90160 TOMATINE 0.435 RT440A FOUNTAIN SOLUTION,DRIED 105C,SMEARED ON DIS 0.434 RT456A FOUNTAIN SOLUTION,DRIED 105C,SMEARED ON D 0.428 AP0021 METHYL CELLULOSE 0.423 RT404A M3 STARCH, KBR GRIND 0.421 AP0024 NYLON 6 (POLYCARPROLACTAM) 0.421 AP0026 NYLON 6/9 (POLYHEXAMETHYLENE NONANEDIAMIDE) 0.415 RT594A CHEESE SPREAD SINGLES ON KBR DISC 0.413 F44203 DODECYL-B-D-GLUCOPYRANOSIDE 0.411 RT026A HYDROCHLORIC CASEIN EX PETRIE 0.409 RT405A M4 CAT STARCH - KBR GRIND 0.409 F41650 METHYL SULFOXIDE


0.916 RT366A HDPE-MULTI REFLECTION SURFACE TRANSMISS 0.880 RT118A 2.5% E.V.A MULTI REFLECTION SURFACE TRANS 0.875 RT369A X03(4202) EX EXTHENE, MIR 0.862 RT368A E220 FILM, EX EXTHENE MIR 0.834 AP0049 POLYETHYLENE HIGH DENSITY 0.833 RT367A SURLYN MULTI REFLECTION SURFACE TRANSMI

0.831 RT338A DEFOAMER, DEAIREX 8042 SOLID, SMEARED ON 0.815 RT261A SYNTHETIC WAX EX CRODA, CHLOROFORM EXTRA 0.813 RT337A DEFOAMER FOAMASTER SOLID, SMEARED ON DISC 0.779 RT297A LDPE NCPE 7530 EX NESTE (TALC FILLED), THIN 0.774 AP0038 POLYAMIDE RESIN MELTING PT 95DEG C 0.770 AP0056 POLYETHYLENE, OXIDIZED ACID NUMBER 15 MG KOH/G 0.766 RT227A HDPE SCLAIR 68A EX DUPONT -THIN FILM 0.765 RT082A HC WAX 181-185 - HOT MELT ADHESIVE COMPON 0.754 RT296A HDPE 7541 NCPE 7541 EX NESTE , T-610626, T 0.743 RT419A WAX EX GULLOTINE M3, CHLOROFORM EXTRACT 0.742 RT362A FOAMASTER DF215 ANTIFOAM EX PETRIE 0.730 RT270A F2 WET END DEFOAMER, CHLOROFORM EXTRACT 0.727 RT257A PARAFFIN WAX EX CRODA, CHLOROFORM EXTRACT 0.721 RT061A RIEGEL SUPER BARRIER LINER 0.718 RT036A POLYETHYLENE-TRANSMISSION FILM 0.717 RT186A PARACOL WAX, THIN FILM 0.716 RT250A LLDPE DOWLEX 3010 EX DOW CHEM. -THIN FILM 0.715 RT190A ALKATHENE -SPECACARD A RING -2 FOILS 0.711 RT119A E220 FILM EX EXTHENE, TRANSMISSION 0.708 F74752 OCTADECYLAMINE 0.707 RT191A NEWS 2617T -SPECACARD -A RING,2 FOILS 0.700 RT120A X03 (4202) EX EXTHENE, TRANSMISSION


0.635 A10000 0.614 RT448A ALUM ALUMINATES, KBR GRIND 0.603 F49790 L(-)-GLYCERALDEHYDE UNNATURAL FORM 0.599 F87495 TETRAHYDROTHIOPHENE 1-OXIDE 0.599 RT406A M4 STARCH - KBR GRIND 0.593 RT405A M4 CAT STARCH - KBR GRIND 0.592 RT404A M3 STARCH, KBR GRIND 0.561 RT178A COLLOID 15/40 0.559 RT125A MG BL. BAG KRAFT (AUTOCLAVING) : MIR(CMIR 0.555 RT579A XEROX FUSER LUBRICANT DOCUTECH (POLYDIMETHYSILOXANE) 0.548 F13170 BENZYL ALCOHOL 0.542 F09326 3-AMINOPROPYLTRIMETHOXYSILANE, 0.542 F56760 3-(2-IMIDAZOLIN-1-YL)PROPYLTRIETHOXYSILANE 0.537 RT037A TALC GROUND WITH KBR 0.531 RT438A SILICA AR GRADE SIO2.XH2O KBR GRIND 0.520 RT280A MIR OF LINER WITH COLOURCOAT WHITE C88/35 0.515 AP0053 POLYETHYLENE, CHLORINATED 48% CHLORINE 0.513 F14880 BIS(2-HYDROXYETHYL)AMINO-TRIS(HYDROXYMETHYL)METHANE 0.510 RT359A COATES GVM6180 - GRAVURE APPLIED NITROCE 0.504 F65540 METHYL ALCOHOL 0.501 RT063A POLYPROPYLENE (TALC FILLED) EX B&D FAN HO 0.494 RT187A TALC PLUS ENSO EXTRACTIVES (ADDED SPECTR 0.492 RT043A ALUM EX F3 : DRIED ON GLASS & GROUND WITH 0.489 RT179A MANILDRA SIZE PRESS STARCH AHF 180 -KBR 0.488 RT363A ALUMINIUM HYDROXIDE 0.482 F28707 B-CYCLODEXTRIN 0.476 RT278A ACF 129 STANDARD BLACK EX PACIFIC INKS, 0.469 RT274A STARCH EX M/V #3 22/4/88 POWDER KBR GRIND

prevalence of microplastics in the seal colony of seal rocks victoria australia

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